CN113229930B

CN113229930B - Electrode needle, ablation equipment, ablation method, device and storage medium

Info

Publication number: CN113229930B
Application number: CN202110680764.XA
Authority: CN
Inventors: 汪龙; 衷兴华; 严韩平; 杨克
Original assignee: Hangzhou Vena Anke Medical Technology Co ltd
Current assignee: Hangzhou Vena Anke Medical Technology Co ltd
Priority date: 2021-06-18
Filing date: 2021-06-18
Publication date: 2023-09-22
Anticipated expiration: 2041-06-18
Also published as: CN113229930A

Abstract

The embodiment of the application provides an electrode needle, ablation equipment, an ablation method, an ablation device and a storage medium. The electrode needle includes: an information processing unit for communication connection with the pulse generator; the conductive needle tube is electrically connected with the pulse generator and outputs an irreversible electroporation ablation pulse sequence to the target tissue; the biological tissue information sensor is at least partially sleeved in the conductive needle tube in an insulating way, is in communication connection with the information processing unit, and is used for collecting biological tissue information of target tissues and sending the biological tissue information to the information processing unit. The embodiment of the application realizes the adaptive adjustment of the irreversible electroporation ablation pulse sequence aiming at the tumor microenvironment, thereby improving the ablation effect.

Description

Electrode needle, ablation equipment, ablation method, device and storage medium

Technical Field

The application relates to the technical field of medical equipment, in particular to an electrode needle, ablation equipment, an ablation method, an ablation device and a storage medium.

Background

Irreversible electroporation ablation is a new tumor ablation technique that uses high voltage short pulse discharge to cause nanoscale perforation of cell membranes, resulting in apoptosis, and is therefore considered a "molecular ablation". From experience of clinical feedback of medicine, the technique is a non-thermal ablation technique, and has the advantages of clear ablation zone limit, capability of reserving nerves of an ablated zone, important tissue structures such as a large blood vessel, a ureter, a bronchus, a large bile duct, a gastrointestinal wall and the like, no influence of heat or cold absorption of blood flow, short ablation time and the like.

Research shows that the effect of electroporation ablation is related to the tumor microenvironment, but the existing irreversible electroporation ablation cannot be adaptively adjusted aiming at the tumor microenvironment, and the ablation effect is limited.

Disclosure of Invention

Aiming at the defects of the existing mode, the application provides an electrode needle, ablation equipment, an ablation method, an ablation device and a storage medium, which are used for solving the technical problems that irreversible electroporation ablation cannot be adaptively adjusted to the tumor microenvironment and the ablation effect is limited in the prior art.

In a first aspect, an embodiment of the present application provides an electrode needle, including:

an information processing unit for communication connection with the pulse generator;

the conductive needle tube is electrically connected with the pulse generator and outputs an irreversible electroporation ablation pulse sequence to the target tissue;

the biological tissue information sensor is at least partially sleeved in the conductive needle tube in an insulating way, is in communication connection with the information processing unit, and is used for collecting biological tissue information of target tissues and sending the biological tissue information to the information processing unit.

Optionally, the biological tissue information sensor includes: at least one of a pH sensor assembly, a conductivity sensor assembly and a temperature sensor assembly;

The pH sensor assembly includes: the pH value sensing probe is at least partially sleeved in the conductive needle tube in an insulating way, and the pH value converter is in communication connection with the information processing unit; the pH value sensing probe is used for collecting the pH value of the target tissue and transmitting the pH value to the pH value converter, and the pH value converter is used for converting the pH value into pH value information and transmitting the pH value information to the information processing unit;

the conductivity sensing assembly includes: the conductivity sensing probe and the conductivity converter are connected; at least part of the conductivity sensing probe is sleeved in the conductive needle tube in an insulating way, and the conductivity converter is in communication connection with the information processing unit; the conductivity sensing probe is used for acquiring the conductivity of the target tissue and transmitting the conductivity to the conductivity converter, and the conductivity converter is used for converting the conductivity into conductivity information and transmitting the conductivity information to the information processing unit;

the temperature sensing assembly includes: the temperature sensing probe and the temperature converter are connected; at least part of the temperature sensing probe is sleeved in the conductive needle tube in an insulating way, and the temperature converter is in communication connection with the information processing unit; the temperature sensing probe is used for acquiring the temperature of the target tissue and transmitting the temperature to the temperature converter, and the temperature converter is used for converting the temperature into temperature information and transmitting the temperature information to the information processing unit.

Optionally, the electrode needle further comprises: a handle;

one part of the conductive needle tube is sleeved in the handle in an insulating way and is used for being electrically connected with the pulse generator, and the other part of the conductive needle tube is positioned outside the handle and is used for extending into target tissues and outputting irreversible electroporation ablation pulse sequences; and/or at least one of the pH value converter, the conductivity converter, the temperature converter and the information processing unit is arranged in the handle.

Optionally, the resistance value of the conductive needle tube is not more than 1 ohm; and/or the material of the conductive needle tube is nickel-titanium alloy or stainless steel.

In a second aspect, embodiments of the present application provide an ablation device comprising: a pulse generator, and an electrode needle as provided in the first aspect above;

the conductive needle tube in the electrode needle is electrically connected with the pulse generator;

the information processing unit in the electrode needle is in communication connection with the pulse generator.

In a third aspect, an embodiment of the present application provides an ablation method, including:

acquiring biological tissue information of a target tissue;

determining an ablation scheme according to the biological tissue information;

according to the ablation protocol, an irreversible electroporation ablation pulse sequence is output to the target tissue.

Optionally, acquiring biological tissue information of the target tissue includes:

At least one of pH value information, conductivity information and temperature information of a target tissue is acquired.

Optionally, determining the ablation plan according to the biological tissue information includes: and determining an ablation scheme according to one or two of pH value information, conductivity information and temperature information.

Optionally, after outputting the irreversible electroporation ablation pulse sequence to the target tissue according to the ablation scheme, further comprising:

adjusting an ablation scheme according to the other two or the other one of the pH value information, the conductivity information and the temperature information;

and outputting an irreversible electroporation ablation pulse sequence with the adjustment parameters to the target tissue according to the adjusted ablation scheme.

In a fourth aspect, embodiments of the present application provide an ablation device comprising:

the information acquisition module is used for acquiring biological tissue information of the target tissue;

the information processing module is used for determining an ablation scheme according to the biological tissue information;

and the pulse output module is used for outputting an irreversible electroporation ablation pulse sequence to the target tissue according to the ablation scheme.

In a fifth aspect, embodiments of the present application provide a computer readable storage medium having a computer program stored thereon, which when executed by a processor, implements the ablation method as provided in the third aspect.

The electrode needle and the ablation equipment provided by the embodiment of the application have the beneficial technical effects that: the biological tissue information sensor can collect biological tissue information of target tissues in real time and send the biological tissue information to the information processing unit, the information processing unit can adjust an ablation scheme according to the real-time biological tissue information and control the conductive needle tube to output a corresponding irreversible electroporation ablation pulse sequence to the target tissues, so that the irreversible electroporation ablation pulse sequence can be adaptively adjusted aiming at tumor microenvironment, and the ablation effect is improved.

The ablation method, the ablation device and the storage medium provided by the embodiment of the application have the beneficial technical effects that: in the ablation process, an ablation scheme is determined according to biological tissue information of target tissues acquired in real time, irreversible electroporation ablation is carried out on the target tissues, and therefore an irreversible electroporation ablation pulse sequence can be adjusted adaptively according to tumor microenvironments, and the ablation effect is improved.

Additional aspects and advantages of the application will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the application.

Drawings

The foregoing and/or additional aspects and advantages of the application will become apparent and readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings, in which:

Fig. 1 is a schematic view of a structural frame of an electrode needle according to an embodiment of the present application;

FIG. 2 is a schematic view of a structural frame of another electrode needle according to an embodiment of the present application;

FIG. 3 is a schematic diagram of a structural framework of an information processing unit according to an embodiment of the present application;

fig. 4 is a schematic structural view of an electrode needle according to an embodiment of the present application;

FIG. 5 is a schematic cross-sectional view of the A-A plane of FIG. 4;

FIG. 6 is a schematic cross-sectional view of the B-B plane of FIG. 4;

fig. 7 is a schematic view of a structural frame of an ablation device according to an embodiment of the present application;

FIG. 8 is a schematic flow chart of an ablation method according to an embodiment of the present application;

FIG. 9 is a schematic flow chart of an ablation method according to an embodiment of the present application;

fig. 10 is a schematic structural frame diagram of an ablation device according to an embodiment of the present application.

In the figure:

10-an ablation device;

100-electrode needle;

110-an information processing unit; a 111-processor; 112-bus; 113-a memory; 114-a transceiver; 115-an input unit; 116-an output unit;

120-conductive needle tube;

130-biological tissue information sensor;

131-ph sensor assembly; 1311-ph sensing probe; 1312-ph converter;

132-a conductivity sensing assembly; 1321-conductivity sensing probe; 1322-conductivity converter;

133-a temperature sensing assembly; 1331—temperature sensing probe; 1332-temperature converter;

140-handle;

200-pulse generator;

20-an ablation device; 21-an information acquisition module; 22-an information processing module; 23-pulse output module.

Detailed Description

The present application is described in detail below, examples of embodiments of the application are illustrated in the accompanying drawings, wherein the same or similar reference numerals refer to the same or similar components or components having the same or similar functions throughout. Further, if detailed description of the known technology is not necessary for the illustrated features of the present application, it will be omitted. The embodiments described below by referring to the drawings are illustrative only and are not to be construed as limiting the application.

It will be understood by those skilled in the art that all terms (including technical and scientific terms) used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs unless defined otherwise. It will be further understood that terms, such as those defined in commonly used dictionaries, should be interpreted as having a meaning that is consistent with their meaning in the context of the prior art and will not be interpreted in an idealized or overly formal sense unless expressly so defined herein.

As used herein, the singular forms "a", "an", "the" and "the" are intended to include the plural forms as well, unless expressly stated otherwise, as understood by those skilled in the art. It will be further understood that the terms "comprises" and/or "comprising," when used in this specification, specify the presence of stated features, integers, steps, operations, elements, and/or components, but do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, and/or groups thereof. It will be understood that when an element is referred to as being "connected" or "coupled" to another element, it can be directly connected or coupled to the other element or intervening elements may also be present. Further, "connected" or "coupled" as used herein may include wirelessly connected or wirelessly coupled. The term "and/or" as used herein includes all or any element and all combination of one or more of the associated listed items.

The inventor of the application researches and discovers that the effect of irreversible electroporation ablation is related to the tumor microenvironment, but the existing electrode needle generally only has the function of applying an irreversible electroporation electric field to the tumor and does not have the capability of sensing the tumor microenvironment, so that the existing irreversible electroporation ablation cannot be adaptively adjusted to the tumor microenvironment, and the ablation effect is limited.

The application provides an electrode needle, an ablation device, an ablation method, an ablation device and a storage medium, and aims to solve the technical problems in the prior art.

The following describes the technical scheme of the present application and how the technical scheme of the present application solves the above technical problems in detail with specific embodiments.

Based on the same inventive concept, an embodiment of the present application provides an electrode needle 100, and a schematic structural frame of the electrode needle 100 is shown in fig. 1, including but not limited to: an information processing unit 110, a conductive needle cannula 120 and a biological tissue information sensor 130.

The information processing unit 110 is adapted to be communicatively connected to the pulse generator 200.

Conductive needle cannula 120 is configured to electrically connect to pulse generator 200 and output an irreversible electroporation ablation pulse sequence to the target tissue.

The biological tissue information sensor 130 is at least partially sleeved in the conductive needle tube 120 in an insulating manner, is in communication connection with the information processing unit 110, and is used for acquiring biological tissue information of the target tissue and sending the biological tissue information to the information processing unit 110.

In this embodiment, the biological tissue information sensor 130 may collect biological tissue information of the target tissue in real time and send the biological tissue information to the information processing unit 110, and the information processing unit 110 may adjust an ablation scheme according to the real-time biological tissue information and control the conductive needle tube 120 to output a corresponding irreversible electroporation ablation pulse sequence to the target tissue, that is, the irreversible electroporation ablation pulse sequence can be adaptively adjusted according to the tumor microenvironment, thereby improving the ablation effect.

During the output of the irreversible electroporation ablation pulse sequence by the electrode needle 100 to the target tissue: permanent nanopores are generated on the cell membrane of target tissue (i.e., tissue in focal zone), the leakage of intracellular substances is excessive or the closing of the cell membrane is excessively slow, irreversible damage is caused to cells, and cells death is caused by perturbing the cell homeostasis, but not necrosis caused by other ablation systems through heat energy or radiation.

Optionally, conductive needle cannula 120 has a resistance value of no greater than 1 ohm. Thus, the conductive needle tube 120 can have good conductivity and is beneficial to outputting irreversible electroporation ablation pulse sequences.

Optionally, conductive needle cannula 120 is nickel titanium alloy or stainless steel. Conductive needle cannula 120 may have good flexibility to facilitate penetration of biological organs or tissues. For example: 304 stainless steel or 316L stainless steel.

The present application provides, in an alternative embodiment, an information processing unit 110, the information processing unit 110 shown in fig. 3 including: a processor 111 and a memory 113. Wherein the processor 111 and the memory 113 are electrically connected, such as via a bus 112.

The processor 111 may be a CPU (Central Processing Unit ), general purpose processor, DSP (Digital Signal Processor, data signal processor), ASIC (Application Specific Integrated Circuit ), FPGA (Field-Programmable Gate Array, field programmable gate array) or other programmable logic device, transistor logic device, hardware component, or any combination thereof. Which may implement or perform the various exemplary logic blocks, modules and circuits described in connection with this disclosure. The processor 111 may also be a combination that implements computing functionality, e.g., comprising one or more microprocessor combinations, a combination of a DSP and a microprocessor, etc.

Bus 112 may include a path to transfer information between the aforementioned components. Bus 112 may be a PCI (Peripheral Component Interconnect, peripheral component interconnect Standard) bus or an EISA (Extended Industry Standard Architecture ) bus, or the like. The bus 112 may be classified as an address bus, a data bus, a control bus, or the like. For ease of illustration, only one thick line is shown in fig. 3, but not only one bus or one type of bus.

The Memory 113 may be, but is not limited to, ROM (Read-Only Memory) or other type of static storage device that can store static information and instructions, RAM (random access Memory ) or other type of dynamic storage device that can store information and instructions, EEPROM (Electrically Erasable Programmable Read Only Memory, electrically erasable programmable Read-Only Memory), CD-ROM (Compact Disc Read-Only Memory) or other optical disk storage, optical disk storage (including compact disc, laser disc, optical disc, digital versatile disc, blu-ray disc, etc.), magnetic disk storage media or other magnetic storage devices, or any other medium that can be used to carry or store desired program code in the form of instructions or data structures and that can be accessed by a computer.

Optionally, the information processing unit 110 may also include a transceiver 114. The transceiver 114 may be used for both reception and transmission of signals. The transceiver 114 may allow the information processing unit 110 to communicate wirelessly or by wire with other devices to exchange data. It should be noted that, in practical application, the transceiver 114 is not limited to one.

Alternatively, the information processing unit 110 may further include an input unit 115. The input unit 115 may be used to receive input digital, character, image, and/or sound information, or to generate key signal inputs related to user settings and function control of the information processing unit 110. The input unit 115 may include, but is not limited to, one or more of a touch screen, a physical keyboard, function keys (such as volume control keys, switch keys, etc.), a track ball, a mouse, a joystick, a camera, a microphone, etc.

Optionally, the information processing unit 110 may further include an output unit 116. The output unit 116 may be used to output or present information processed by the processor 111. The output unit 116 may include, but is not limited to, one or more of a display device, a speaker, a vibration device, and the like.

While fig. 3 shows information processing unit 110 with various devices, it is to be understood that not all illustrated devices are required to be implemented or provided. More or fewer devices may be implemented or provided instead.

The biological tissue information may include at least one of ph information, conductivity information, and temperature information of the target tissue microenvironment.

Optionally, as shown in fig. 2, the biological tissue information sensor 130 according to the embodiment of the present application includes: at least one of a ph sensor assembly 131, a conductivity sensor assembly 132, and a temperature sensor assembly 133.

The inventor of the present application considers that the effect of irreversible electroporation ablation is related to the tumor microenvironment PH (PH value), but the existing electrode needle 100 generally only has the function of applying an electroporation electric field to a tumor and does not have the capability of sensing the tumor microenvironment PH, so that the existing irreversible electroporation ablation cannot be adaptively adjusted to the tumor microenvironment PH, and the ablation effect is limited. To this end, the present application provides one possible implementation of the biological tissue information sensor 130 as follows:

as shown in fig. 2, the biological tissue information sensor 130 according to the embodiment of the present application includes, but is not limited to: and a ph sensor assembly 130.

The ph sensor assembly 130 includes, but is not limited to: a pH sensor probe 1311 and a pH converter 1312 are connected. As shown in fig. 5 and 6, the ph sensing probe 1311 is at least partially sleeved in the conductive needle tube 120, and the ph converter 1312 is communicatively connected to the information processing unit 110. The ph sensing probe 1311 is used to collect the ph of the target tissue and transmit it to the ph converter 1312, where the ph converter 1312 is used to convert the ph into ph information and send it to the information processing unit 110.

In this embodiment, the PH sensing probe 1311 in the PH sensor assembly 130 can collect the PH of the target tissue in real time and transmit the collected PH to the PH converter 1312, the PH converter 1312 in the PH sensor assembly 130 can convert the PH into PH information and send the information to the information processing unit 110, the information processing unit 110 can adjust the ablation scheme according to the real-time PH information and control the conductive needle tube 120 to output a corresponding irreversible electroporation ablation pulse sequence to the target tissue, that is, can realize PH adaptive adjustment of the irreversible electroporation ablation pulse sequence for the tumor microenvironment, thereby improving the ablation effect.

Alternatively, the ph sensing probe 1311 may employ: PH sensing probes of the OAKTON model in U.S.A.

Alternatively, pH sensing probe 1311 may be partially exposed to conductive needle cannula 120 or may be located at a port of conductive needle cannula 120.

Alternatively, the ph converter 1312 may employ a PCB (printed circuit board) that converts ph (analog signal) into ph information (digital signal).

The inventor of the present application considers that the effect of irreversible electroporation ablation is also related to the tumor microenvironment conductivity, but the existing electrode needle 100 generally only has the function of applying an electroporation electric field to a tumor and does not have the capability of sensing the tumor microenvironment conductivity, so that the existing irreversible electroporation ablation cannot be adaptively adjusted to the tumor microenvironment conductivity, and the ablation effect is limited. To this end, the present application provides one possible implementation of the biological tissue information sensor 130 as follows: as shown in fig. 2, the biological tissue information sensor 130 according to the embodiment of the present application includes, but is not limited to: a conductivity sensing assembly 132.

Conductivity sensing assembly 132 includes, but is not limited to: connected conductivity sense probe 1321 and conductivity transducer 1322. As shown in fig. 5 and 6, the conductivity sensing probe 1321 is at least partially and electrically sleeved in the conductive needle cannula 120, and the conductivity converter 1322 is communicatively connected to the information processing unit 110. The conductivity sensing probe 1321 is used to collect the conductivity of the target tissue and transmit to the conductivity converter 1322, and the conductivity converter 1322 is used to convert the conductivity into conductivity information and send it to the information processing unit 110.

In this embodiment, the conductivity sensing probe 1321 in the conductivity sensing component 132 can collect the conductivity of the target tissue in real time and transmit the conductivity to the conductivity converter 1322, the conductivity converter 1322 in the conductivity sensing component 132 can convert the conductivity into conductivity information and send the conductivity information to the information processing unit 110, the information processing unit 110 can adjust the ablation scheme according to the real-time conductivity information and control the conductive needle tube 120 to output a corresponding irreversible electroporation ablation pulse sequence to the target tissue, that is, the irreversible electroporation ablation pulse sequence can be adaptively adjusted according to the conductivity of the tumor microenvironment, and the ablation effect is improved.

It can be understood that the information processing unit 110 can comprehensively adjust the ablation scheme according to the real-time ph information and the real-time conductivity information at the same time, that is, comprehensively consider the ph and the conductivity of the tumor microenvironment, adaptively adjust the irreversible electroporation ablation pulse sequence, and further improve the ablation effect.

Alternatively, the conductivity sensing probe 1321 may employ: three qi electrons.

Alternatively, conductivity sensing probe 1321 may be partially exposed to conductive needle cannula 120 or may be located at a port of conductive needle cannula 120.

Alternatively, the conductivity converter 1322 may employ a PCB (printed circuit board) that converts conductivity (analog signals) into conductivity information (digital signals).

The inventor of the present application considers that the effect of irreversible electroporation ablation is also related to the tumor microenvironment temperature, but the existing electrode needle 100 generally only has the function of applying an electroporation electric field to the tumor and does not have the capability of sensing the tumor microenvironment temperature, so that the existing irreversible electroporation ablation cannot be adaptively adjusted to the tumor microenvironment temperature, and the ablation effect is limited. To this end, the present application provides one possible implementation of the biological tissue information sensor 130 as follows:

As shown in fig. 2, the biological tissue information sensor 130 according to the embodiment of the present application includes, but is not limited to: a temperature sensing assembly 133.

Temperature sensing assembly 133 includes, but is not limited to: a temperature sensing probe 1331 and a temperature transducer 1332 are connected. As shown in fig. 5 and 6, temperature sensing probe 1331 is at least partially and electrically sleeved within conductive needle cannula 120, and temperature transducer 1332 is communicatively coupled to information processing unit 110. The temperature sensing probe 1331 is used for acquiring the temperature of the target tissue in real time and transmitting the temperature to the temperature converter 1332, and the temperature converter 1332 is used for converting the temperature into temperature information and transmitting the temperature information to the information processing unit 110.

In this embodiment, the temperature sensing probe 1331 in the temperature sensing component 133 can collect the temperature of the target tissue in real time and transmit the temperature to the temperature converter 1332, the temperature converter 1332 in the temperature sensing component 133 can convert the temperature into temperature information and send the temperature information to the information processing unit 110, the information processing unit 110 can adjust the ablation scheme according to the real-time temperature information and control the conductive needle tube 120 to output a corresponding irreversible electroporation ablation pulse sequence to the target tissue, that is, the irreversible electroporation ablation pulse sequence can be adjusted adaptively according to the temperature of the tumor microenvironment, and the ablation effect is improved.

It can be understood that the information processing unit 110 can comprehensively adjust the ablation scheme according to the real-time ph information and the real-time temperature information at the same time, that is, comprehensively consider the ph and the temperature of the tumor microenvironment, adaptively adjust the irreversible electroporation ablation pulse sequence, and further improve the ablation effect. The information processing unit 110 can also comprehensively adjust the ablation scheme according to the real-time pH value information, the conductivity information and the real-time temperature information at the same time, so that comprehensive consideration of the pH value, the conductivity and the temperature of the tumor microenvironment can be realized, the irreversible electroporation ablation pulse sequence can be adaptively adjusted, and the ablation effect can be further improved.

Alternatively, the temperature sensing probe 1331 may employ: TT-K-40 model temperature sensing probe.

Alternatively, temperature sensing probe 1331 may partially expose conductive needle cannula 120 or may be located at a port of conductive needle cannula 120.

Alternatively, the temperature converter 1332 may employ a PCB (printed circuit board) that converts temperature (analog signal) into temperature information (digital signal).

The inventors of the present application considered that the electrode needle 100 needs to be easy to hold in order to facilitate the operation such as puncturing by the operator. To this end, the present application provides the electrode needle 100 with one possible implementation as follows:

As shown in fig. 4, the electrode needle 100 of the embodiment of the present application further includes, but is not limited to: a handle 140.

A portion of the conductive needle cannula 120 is disposed within the handle 140 in an insulated manner and is adapted to be electrically connected to the pulse generator 200, and another portion of the conductive needle cannula 120 is disposed outside the handle 140 for extending into the target tissue and outputting an irreversible electroporation ablation pulse sequence.

In the present embodiment, the handle 140 can be easily held by an operator, so that the operator can more easily control the electrode needle 100 when performing operations such as puncturing, and the operability of the electrode needle 100 can be improved. Also, the interior space of the handle 140 provides a place for the electrical connection between the electrode needle 100 and the pulse generator 200.

Optionally, the handle 140 is made of an insulating material.

Optionally, at least one of the ph converter 131, the conductivity converter 1322, the temperature converter 1332, and the information processing unit 110 is disposed within the handle 140.

An embodiment of the present application provides an ablation device 10, a schematic structural frame of which ablation device 10 is shown in fig. 7, including but not limited to: a pulse generator 200, and any of the electrode pins 100 provided by the previous embodiments.

Conductive needle cannula 120 in electrode needle 100 is electrically connected to pulser 200.

The information processing unit 110 in the electrode needle 100 is communicatively connected to the pulse generator 200.

In this embodiment, the pulse generator 200 outputs an electrical pulse sequence to the conductive needle tube 120 in the electrode needle 100, so that the conductive needle tube 120 can generate permanent nanopores on the cell membrane of the target tissue (i.e. the tissue in the focal zone), the leakage of the intracellular substances is too serious or the closing of the cell membrane is too slow, which causes irreversible damage to the cells, and the cells die by disturbing the cell homeostasis, but not necrosis caused by heat energy or radiation by other ablation systems.

In some possible embodiments, the information processing unit 110 in the electrode needle 100 is in communication connection with an upper computer, and at this time, the upper computer can implement program update or data backup of the information processing unit 110 in the electrode needle 100, and also can implement remote control of the electrode needle 100, so as to facilitate function expansion of the ablation device 10.

Optionally, the upper computer is communicatively connected to the information processing unit 110 in the electrode needle 100 through WIFI (Wireless Fidelity, wireless fidelity, also called mobile hotspot).

Optionally, the upper computer is communicatively connected to the information processing unit 110 in the electrode needle 100 through the cloud.

In some possible embodiments, the information processing unit 110 in the electrode needle 100 is communicatively connected to an information display device, at which point the information display device may effect display to an operator: at least one of biological tissue information of the target tissue, an ablation protocol, and specific parameters of the output irreversible electroporation ablation pulse sequence. The method is beneficial to the timely grasp of the operator on the melting state.

Based on the same inventive concept, an embodiment of the present application provides an ablation method, a flow chart of which is shown in fig. 8, including but not limited to steps S101-S103:

s101: biological tissue information of a target tissue is acquired.

Alternatively, the information processing unit 110 in the electrode needle 100 provided by the foregoing embodiment of the present application may be employed to acquire biological tissue information of the target tissue. The biological tissue information may be obtained from biological tissue information of the target tissue acquired in real time by the biological tissue information sensor 130.

Alternatively, the acquiring biological tissue information of the target tissue in the present step S101 may include: at least one of pH value information, conductivity information and temperature information of a target tissue is acquired.

S102: and determining an ablation scheme according to the biological tissue information.

Alternatively, the information processing unit 110 in the electrode needle 100 provided by the foregoing embodiment of the present application may be employed to determine an ablation scheme based on biological tissue information.

Optionally, the ablation protocol includes, but is not limited to, specific types and/or specific parameters of irreversible electroporation ablation pulse sequences to be outputted (or formulated).

S103: according to the ablation protocol, an irreversible electroporation ablation pulse sequence is output to the target tissue.

Alternatively, the information processing unit 110 in the electrode needle 100 provided by the foregoing embodiment of the present application may control the pulse generator 200 to generate a corresponding irreversible electroporation ablation pulse sequence according to an ablation scheme, and the irreversible electroporation ablation pulse sequence is output to the target tissue by the conductive needle tube 120.

According to the ablation method provided by the embodiment, an ablation scheme can be determined according to biological tissue information of target tissues acquired in real time in an ablation process, and then irreversible electroporation ablation is performed on the target tissues according to the ablation scheme, so that an irreversible electroporation ablation pulse sequence can be adaptively adjusted according to tumor microenvironments, and the ablation effect is improved.

Based on the same inventive concept, an embodiment of the present application provides a deployment method of an ablation method, a flow chart of which is shown in fig. 9, and the method includes, but is not limited to, steps S201-S205:

S201: and acquiring pH value information, conductivity information and temperature information of the target tissue.

Alternatively, the information processing unit 110 in the electrode needle 100 provided in the foregoing embodiment of the present application may be used to obtain ph information, conductivity information, and temperature information of the target tissue.

The ph information may be obtained by converting the ph of the target tissue collected in real time by the ph converter 132 according to the ph sensing probe 1311; conductivity information may be obtained by conductivity converter 1322 by collecting conductivity conversion of the target tissue in real time from conductivity sensing probe 1321; the temperature information may be obtained by the temperature converter 1332 by real-time acquisition of temperature conversions of the target tissue based on the temperature sensing probe 1331.

S202: and determining an ablation scheme according to one or two of pH value information, conductivity information and temperature information.

Alternatively, the information processing unit 110 in the electrode needle 100 provided in the foregoing embodiment of the present application may determine the ablation scheme according to one or two of ph information, conductivity information, and temperature information.

In one example, an ablation protocol is determined based on the ph information.

In one example, an ablation protocol is determined based on the ph information and the conductivity information.

In one example, an ablation protocol is determined based on the ph information and the temperature information.

In one example, an ablation protocol is determined based on the conductivity information and the temperature information.

S203: according to the ablation protocol, an irreversible electroporation ablation pulse sequence is output to the target tissue.

S204: and adjusting an ablation scheme according to the other two or another of the pH value information, the conductivity information and the temperature information.

Alternatively, the information processing unit 110 in the electrode needle 100 provided in the foregoing embodiment of the present application may be used to adjust the ablation scheme according to another two or another one of ph information, conductivity information, and temperature information.

In one example, an adjusted ablation protocol is determined based on the conductivity information and the temperature information.

In one example, an adjustment ablation protocol is determined based on temperature information.

In one example, an adjustment ablation protocol is determined based on the conductivity information.

In one example, an adjustment ablation protocol is determined based on the ph information.

S205: and outputting an irreversible electroporation ablation pulse sequence with the adjustment parameters to the target tissue according to the adjusted ablation scheme.

Alternatively, the information processing unit 110 in the electrode needle 100 provided by the foregoing embodiment of the present application may control the pulse generator 200 to generate the irreversible electroporation ablation pulse sequence corresponding to the adjustment parameter according to the adjusted ablation scheme, and the irreversible electroporation ablation pulse sequence of the adjustment parameter is output to the target tissue by the conductive needle tube 120.

In this embodiment, the ablation scheme may be determined and implemented according to one or two of ph information, conductivity information and temperature information of the target tissue microenvironment in real time, and then the ablation scheme may be adjusted and implemented according to the other two or other information of the target tissue microenvironment in real time in the implementation process, so as to effectively improve the adaptability between the irreversible electroporation ablation pulse sequence and the target tissue microenvironment, and further improve the ablation effect.

Based on the same inventive concept, an ablation device 20 is provided according to an embodiment of the present application, and a schematic structural frame of the device is shown in fig. 10, including but not limited to: an information acquisition module 21, an information processing module 22 and a pulse output module 23.

The information acquisition module 21 is used for acquiring biological tissue information of the target tissue.

The information processing module 22 is used for determining an ablation scheme according to the biological tissue information.

The pulse output module 23 is configured to output an irreversible electroporation ablation pulse sequence to the target tissue according to an ablation protocol.

According to the ablation device 20 provided by the embodiment, an ablation scheme can be determined according to biological tissue information of target tissues acquired in real time in an ablation process, irreversible electroporation ablation is performed on the target tissues, so that an irreversible electroporation ablation pulse sequence can be adaptively adjusted aiming at tumor microenvironments, and the ablation effect is improved.

In some possible embodiments, the information acquisition module 21 is configured to acquire biological tissue information of the target tissue, specifically: at least one of pH value information, conductivity information and temperature information of a target tissue is acquired.

In some possible embodiments, the information processing module 22 is configured to, when determining an ablation scheme according to biological tissue information, specifically: and determining an ablation scheme according to one or two of pH value information, conductivity information and temperature information.

In some possible embodiments, after the pulse output module 23 is configured to output the irreversible electroporation ablation pulse sequence to the target tissue according to the ablation protocol, the information processing module 22 is configured to adjust the ablation protocol according to another two or another of the ph information, the conductivity information, and the temperature information. Next, the pulse output module 23 is further configured to: and outputting an irreversible electroporation ablation pulse sequence with the adjustment parameters to the target tissue according to the adjusted ablation scheme.

Based on the same inventive concept, embodiments of the present application provide a computer-readable storage medium having a computer program stored thereon, which when executed by a processor, implements various alternative implementations of the ablation method provided by the embodiments of the present application.

It will be appreciated by those skilled in the art that the computer readable storage media provided by the present embodiments can be any available media that can be accessed by an electronic device, including but not limited to volatile and nonvolatile media, removable or non-removable media. Computer readable storage media includes, but is not limited to, any type of disk including, but not limited to, floppy disks, hard disks, optical disks, CD-ROMs, and magneto-optical disks, ROM, RAM, EPROM (Erasable Programmable Read-Only Memory ), EEPROMs (Electrically Erasable Programmable Read Only Memory, electrically erasable programmable read-Only Memory), flash Memory, magnetic cards, or optical cards. That is, computer-readable storage media includes, but is not limited to, any medium that stores or transmits information in a form readable by a device (e.g., a computer).

The embodiment of the present application provides various optional implementations of a computer readable storage medium suitable for any of the above ablation methods, which are not described herein.

By applying the embodiment of the application, at least the following beneficial effects can be realized:

1. the electrode needle and the ablation equipment provided by the embodiment of the application have the beneficial technical effects that: the biological tissue information sensor 130 can collect biological tissue information of the target tissue in real time and send the biological tissue information to the information processing unit 110, the information processing unit 110 can adjust an ablation scheme according to the real-time biological tissue information and control the conductive needle tube 120 to output a corresponding irreversible electroporation ablation pulse sequence to the target tissue, so that the irreversible electroporation ablation pulse sequence can be adaptively adjusted according to the tumor microenvironment, and the ablation effect is improved.

2. The ph sensor assembly 131, the conductivity sensor assembly 132 and the temperature sensor assembly 133 can respectively collect ph information, conductivity information and temperature information in real time, and the information processing unit 110 can simultaneously adjust an ablation scheme comprehensively according to at least one of the real-time ph information, conductivity information and temperature information and in combination with the real-time pressure information, and adaptively adjust an irreversible electroporation ablation pulse sequence, thereby improving an ablation effect.

3. The ablation method, the ablation device and the storage medium provided by the embodiment of the application have the beneficial technical effects that: in the ablation process, an ablation scheme is determined according to biological tissue information of target tissues acquired in real time, irreversible electroporation ablation is carried out on the target tissues, and therefore an irreversible electroporation ablation pulse sequence can be adjusted adaptively according to tumor microenvironments, and the ablation effect is improved.

Those of skill in the art will appreciate that the various operations, methods, steps in the flow, acts, schemes, and alternatives discussed in the present application may be alternated, altered, combined, or eliminated. Further, other steps, means, or steps in a process having various operations, methods, or procedures discussed herein may be alternated, altered, rearranged, disassembled, combined, or eliminated. Further, steps, measures, schemes in the prior art with various operations, methods, flows disclosed in the present application may also be alternated, altered, rearranged, decomposed, combined, or deleted.

In the description of the present application, it should be understood that the terms "center," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like indicate orientations or positional relationships based on the orientation or positional relationships shown in the drawings, merely to facilitate describing the present application and simplify the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present application.

The terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more such feature. In the description of the present application, unless otherwise indicated, the meaning of "a plurality" is two or more.

In the description of the present application, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present application will be understood in specific cases by those of ordinary skill in the art.

In the description of the present specification, a particular feature, structure, material, or characteristic may be combined in any suitable manner in one or more embodiments or examples.

It should be understood that, although the steps in the flowcharts of the figures are shown in order as indicated by the arrows, these steps are not necessarily performed in order as indicated by the arrows. The steps are not strictly limited in order and may be performed in other orders, unless explicitly stated herein. Moreover, at least some of the steps in the flowcharts of the figures may include a plurality of sub-steps or stages that are not necessarily performed at the same time, but may be performed at different times, the order of their execution not necessarily being sequential, but may be performed in turn or alternately with other steps or at least a portion of the other steps or stages.

The foregoing is only a partial embodiment of the present application, and it should be noted that it will be apparent to those skilled in the art that modifications and adaptations can be made without departing from the principles of the present application, and such modifications and adaptations are intended to be comprehended within the scope of the present application.

Claims

1. An electrode needle, comprising: the device comprises a biological tissue information sensor, an information processing unit and a conductive needle tube;

the biological tissue information sensor is at least partially sleeved in the conductive needle tube in an insulating way and is in communication connection with the information processing unit; the biological tissue information sensor is configured to acquire biological tissue information of a target tissue and transmit the biological tissue information to the information processing unit; the biological tissue information sensor is configured to acquire ph information and temperature information of a target tissue, or acquire conductivity information and temperature information of the target tissue, or acquire ph information, conductivity information and temperature information of the target tissue;

the information processing unit is configured to be in communication connection with a pulse generator, determine an ablation scheme according to one or two of the pH value information and the conductivity information, and drive the pulse generator to generate an irreversible electroporation ablation pulse sequence according to the ablation scheme;

The conductive needle cannula is configured for electrical connection with the pulse generator and outputting the irreversible electroporation ablation pulse sequence to a target tissue;

the information processing unit is further configured to adjust the ablation scheme according to the temperature information after the conductive needle tube outputs an irreversible electroporation ablation pulse sequence to a target tissue; driving the pulse generator to generate an irreversible electroporation ablation pulse sequence with adjustment parameters according to the adjusted ablation scheme;

the conductive needle cannula is further configured for outputting an irreversible electroporation ablation pulse sequence of the tuning parameter to the target tissue.

2. The electrode needle of claim 1, wherein the biological tissue information sensor comprises: at least one of a pH sensor assembly, a conductivity sensor assembly and a temperature sensor assembly;

the pH sensor assembly includes: the pH value sensing probe is at least partially sleeved in the conductive needle tube in an insulating way, and the pH value converter is in communication connection with the information processing unit; the pH value sensing probe is used for collecting the pH value of target tissues and transmitting the pH value to the pH value converter, and the pH value converter is used for converting the pH value into pH value information and transmitting the pH value information to the information processing unit;

the temperature sensing assembly includes: the temperature sensing probe and the temperature converter are connected; at least part of the temperature sensing probe is sleeved in the conductive needle tube in an insulating way, and the temperature converter is in communication connection with the information processing unit; the temperature sensing probe is used for acquiring the temperature of target tissue and transmitting the temperature to the temperature converter, and the temperature converter is used for converting the temperature into temperature information and transmitting the temperature information to the information processing unit.

3. The electrode needle of claim 2, wherein the electrode needle further comprises: a handle;

one part of the conductive needle tube is sleeved in the handle in an insulating way and is used for being electrically connected with the pulse generator, and the other part of the conductive needle tube is positioned outside the handle and is used for extending into the target tissue and outputting an irreversible electroporation ablation pulse sequence; and/or at least one of the pH value converter, the conductivity converter, the temperature converter and the information processing unit is arranged in the handle.

4. An electrode needle according to any one of claims 1-3, wherein the conductive needle cannula has a resistance value of no more than 1 ohm;

and/or the material of the conductive needle tube is nickel-titanium alloy or stainless steel.

5. An ablation device, comprising: a pulse generator, and an electrode needle as claimed in any one of the preceding claims 1-4;