CN113229783A - Image acquisition system, method and device for fluorescence imaging - Google Patents
Image acquisition system, method and device for fluorescence imaging Download PDFInfo
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0033—Features or image-related aspects of imaging apparatus classified in A61B5/00, e.g. for MRI, optical tomography or impedance tomography apparatus; arrangements of imaging apparatus in a room
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0033—Features or image-related aspects of imaging apparatus classified in A61B5/00, e.g. for MRI, optical tomography or impedance tomography apparatus; arrangements of imaging apparatus in a room
- A61B5/0036—Features or image-related aspects of imaging apparatus classified in A61B5/00, e.g. for MRI, optical tomography or impedance tomography apparatus; arrangements of imaging apparatus in a room including treatment, e.g., using an implantable medical device, ablating, ventilating
Abstract
The invention discloses an image acquisition system, method and device for fluorescence imaging, wherein the image processing method comprises the following steps: at the first time, by acquiring a first image captured when the reference light irradiates the subject, the first image can display the entire clear image and the approximate position and boundary of the tumor in the entire clear image. At the second moment, the object to be shot is irradiated by acquiring exciting light, so that a second image shot when the object to be shot generates fluorescence is generated, the second image can display the accurate position and the boundary of a tumor in the whole blurred image, and finally the fluorescence parts in the first image and the second image are synthesized to generate a first synthesized image, wherein the first synthesized image can display the whole sharp image and can display the accurate position and the boundary of the tumor in the whole sharp image, so that the tumor can be completely resected, and the problem of local tumor recurrence is avoided.
Description
Technical Field
The invention relates to the technical field of image processing, in particular to an image acquisition system, method and device for fluorescence imaging.
Background
The medical imaging greatly improves the accuracy of operators on the reasons, the positions, the disease development stages and the operation treatment methods of the focuses. Existing imaging techniques include X-ray imaging, magnetic resonance imaging, nuclear medicine imaging, and ultrasound imaging, among others. During the operation, the operator can visually judge the position and the boundary of the tumor mainly through the existing imaging technology. For tumors with the same color, the same texture and the same hardness as those of cell tissues, the existing imaging technology can only display the approximate position and the boundary of the tumor in the whole picture, so that the tumor cannot be completely resected, and the problem of local tumor recurrence is easily caused.
Disclosure of Invention
The invention mainly aims to provide an image acquisition system, a method and a device for fluorescence imaging, and aims to solve the technical problem that the imaging technology in the prior art can only display the approximate position and the boundary of a tumor in the whole picture, so that the tumor cannot be completely resected, and local tumor recurrence is easily caused.
In a first aspect, the present application provides an acquisition system for fluorescence imaging, comprising:
a light source assembly comprising a first light source that emits reference light and a second light source that emits excitation light;
the camera shooting device comprises a camera shooting main body and an optical filter movably arranged on the camera shooting main body, wherein at a first moment, the optical filter is in a retracted state so that visible light emitted by the first light source and the second light source respectively irradiates on an object to be shot, and the camera shooting main body is used for shooting the object to be shot under the action of the visible light to obtain a first image; at a second moment, the optical filter is in a use state, so that narrow-band light which is emitted by the first light source and emitted by the second light source and meets conditions is irradiated on an object to be photographed, the object to be photographed generates fluorescence, and the photographing main body is used for photographing the object to be photographed under the action of the narrow-band light to obtain a second image; and
a processor for synthesizing the fluorescent portions in the first and second images to generate a first composite image.
In a second aspect, the present application provides an acquisition system for fluorescence imaging, comprising:
a light source assembly comprising a first light source emitting reference light and a plurality of second light sources emitting excitation light of different wavelengths;
the camera shooting device comprises a camera shooting main body and an optical filter movably arranged on the camera shooting main body, wherein at a first moment, the optical filter is in a retracted state so that visible light emitted by the first light source and the second light source respectively irradiates on an object to be shot, and the camera shooting main body is used for shooting the object to be shot under the action of the visible light to obtain a first image; at a second moment, the optical filter is in a use state, so that narrow-band light which is emitted by the second light sources and meets conditions and has different wavelengths is irradiated on the object to be shot, the object to be shot generates fluorescence with different colors, the shooting main body is used for shooting the object to be shot under the action of the narrow-band light with different wavelengths, and a plurality of second images are obtained, wherein the fluorescence parts in the second images are different colors; and
a processor for combining the fluorescent portions of different colors in the first image and the plurality of second images to generate a first combined image.
In a third aspect, the present application provides an image processing method for fluorescence imaging, comprising:
at a first moment, acquiring a first image shot when a reference light irradiates an object to be shot;
at a second moment, acquiring exciting light to irradiate the object to be shot so as to enable the object to be shot to generate a second image shot when fluorescence is generated;
and synthesizing the fluorescent parts in the first image and the second image to generate a first synthesized image.
Preferably, the image processing method further includes:
at a third moment, acquiring a third image shot when the reference light irradiates the object to be shot;
at a fourth moment, acquiring exciting light to irradiate the object to be shot so as to enable the object to be shot to generate a fourth image shot when fluorescence is generated;
synthesizing the fluorescent parts in the third image and the fourth image to generate a second synthesized image;
and so on;
at the Nth moment, acquiring an Nth image shot when the reference light irradiates the object to be shot;
at the Nth moment, acquiring exciting light to irradiate the object to be shot so as to enable the object to be shot to generate an Nth image shot when fluorescence is generated;
and synthesizing the fluorescence parts in the Nth image and the Nth image to generate an N/2 th synthesized image.
Preferably, the second image is processed to obtain a processed second image.
Preferably, processing the second image comprises:
and adjusting the parameters of the fluorescent part in the second image so that the fluorescent part in the second image meets the requirements.
Preferably, the contrast and brightness of the fluorescent part in the second image are adjusted so that the contrast and brightness of the fluorescent part in the second image meet the requirements.
Preferably, the reference light is white light and the excitation light is narrow band light.
In a fourth aspect, the present application provides an image processing method for fluorescence imaging, comprising:
at a first moment, acquiring a first image shot when a reference light irradiates an object to be shot;
at a second moment, acquiring a plurality of excitation lights with different wavelengths to alternately irradiate the object to be shot so as to enable the object to be shot to generate a plurality of second images shot when fluorescence with different colors is generated, wherein fluorescence parts in the plurality of second images are different colors;
and synthesizing the fluorescent parts with different colors in the first image and the plurality of second images to generate a first synthesized image.
In a fifth aspect, the present application provides an image processing apparatus for fluorescence imaging, comprising:
the first image acquisition module is used for acquiring a first image shot when the reference light irradiates the object to be shot at a first moment;
the second image acquisition module is used for acquiring exciting light to irradiate the object to be shot at a second moment so as to enable the object to be shot to generate a second image shot when fluorescence is generated;
and the first synthetic image module is used for synthesizing the fluorescence parts in the first image and the second image to generate a first synthetic image.
Compared with the prior art, the invention has the following beneficial effects:
at the first time, by acquiring a first image captured when the reference light irradiates the subject, the first image can display the entire clear image and the approximate position and boundary of the tumor in the entire clear image. At the second moment, the object to be shot is irradiated by acquiring exciting light, so that a second image shot when the object to be shot generates fluorescence is generated, the second image can display the accurate position and the boundary of a tumor in the whole blurred image, and finally the fluorescence parts in the first image and the second image are synthesized to generate a first synthesized image, wherein the first synthesized image can display the whole sharp image and can display the accurate position and the boundary of the tumor in the whole sharp image, so that the tumor can be completely resected, and the problem of local tumor recurrence is avoided.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the structures shown in the drawings without creative efforts.
FIG. 1 is a block diagram of an acquisition system for fluorescence imaging according to an embodiment of the present invention;
FIG. 2 is a schematic view of an acquisition device for fluorescence imaging according to an embodiment of the present invention;
FIG. 3 is a flow diagram of an image processing method for fluorescence imaging according to one embodiment of the invention;
FIG. 4 is a flow diagram of an image processing method for fluorescence imaging according to another embodiment of the invention;
fig. 5 is a block diagram of the configuration of an image processing apparatus for fluorescence imaging according to an embodiment of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It should be noted that all directional indicators (such as up, down, left, right, front, and back) in the embodiments of the present invention are only used to explain the relative position relationship between the components, the motion situation, and the like in a specific posture (as shown in the drawings), and if the specific posture is changed, the directional indicator is changed accordingly.
In addition, the descriptions related to "first", "second", etc. in the present invention are for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In addition, "and/or" in the whole text includes three schemes, taking a and/or B as an example, including a technical scheme, and a technical scheme that a and B meet simultaneously; in addition, technical solutions between various embodiments may be combined with each other, but must be realized by a person skilled in the art, and when the technical solutions are contradictory or cannot be realized, such a combination should not be considered to exist, and is not within the protection scope of the present invention.
This application adopts fluorescence imaging technique, through fluorescence reagent mark tumour, makes the position and the border that the art person can pinpoint the tumour in the operation process to thoroughly excise the tumour, reduce patient's secondary injury.
As shown in fig. 1-2, in one embodiment, an acquisition system for fluorescence imaging is provided, the acquisition system comprising a light source assembly 1, an image pickup device 2, and a processor 3, the light source assembly 1 comprising a first light source 11 and a second light source 12, the first light source 11 emitting reference light, the second light source 12 emitting excitation light; the image pickup device 2 comprises an image pickup main body 21 and an optical filter 22 movably arranged on the image pickup main body 21, at a first moment, the optical filter 22 is in a retracted state, so that visible light emitted by the first light source 11 and the second light source 12 respectively irradiates on an object to be photographed, and the image pickup main body 21 is used for photographing the object to be photographed under the action of the visible light to obtain a first image; at a second time, the optical filter 22 is in a use state, so that narrow-band light which is emitted by the first light source 11 and the second light source 12 respectively and meets the conditions is irradiated on an object to be photographed, so that the object to be photographed generates fluorescence, and the photographing main body 21 is used for photographing the object to be photographed under the action of the narrow-band light to obtain a second image; the processor 3 is configured to synthesize the fluorescent portions in the first image and the second image to generate a first synthesized image.
At a first moment, the optical filter 22 is retracted, so that the visible light emitted by the first light source 11 and the visible light emitted by the second light source 12 are irradiated on the object to be photographed, and the main imaging body 21 can capture the object to be photographed under the action of the visible light to obtain a first image, wherein the first image can display the whole clear image and the approximate position and the boundary of the tumor in the whole clear image. At the second moment, the optical filter 22 is used to enable narrow-band light emitted by the first light source 11 and the second light source 12 respectively to irradiate on the object to be photographed, so that the object to be photographed generates fluorescence, the main camera body 21 can shoot the object to be photographed under the action of the narrow-band light to obtain a second image, the second image can display the accurate position and the boundary of the tumor in the whole blurred image, and finally the fluorescence parts in the first image and the second image are synthesized to generate a first synthesized image, the first synthesized image can display the whole blurred image and the accurate position and the boundary of the tumor in the whole blurred image, so that the tumor can be completely cut off, and the problem of local tumor recurrence is avoided.
The main camera body 21 of the present embodiment adopts a single CMOS for image acquisition, and compared with a dual CMOS, the single CMOS has the advantages of higher accuracy, smaller volume, smaller heat generation amount, and the like.
The imaging device 2 of the present embodiment can be used for non-fluorescence surgery, and when performing non-fluorescence surgery, only the optical filter 22 needs to be retracted, and the imaging device 2 does not need to be replaced, thereby improving the versatility of the imaging device 2.
In the present embodiment, the first light source 11 and the second light source 12 simultaneously irradiate the object to be photographed. It will be appreciated that in alternative embodiments, the first light source 11 and the second light source 12 are staggered to illuminate the object to be photographed.
In one embodiment, an acquisition system for fluorescence imaging is provided, the acquisition system includes a light source assembly 1, an image pickup device 2 and a processor 3, the light source assembly 1 includes a first light source 11 and a plurality of second light sources 12, the first light source 11 emits reference light, and the second light sources 12 emit excitation light with different wavelengths; the image pickup device 2 comprises an image pickup main body 21 and an optical filter 22 movably arranged on the image pickup main body 21, at a first moment, the optical filter 22 is in a retracted state, so that visible light emitted by the first light source 11 and the second light source 12 respectively irradiates on an object to be photographed, and the image pickup main body 21 is used for photographing the object to be photographed under the action of the visible light to obtain a first image; at a second time, the optical filter 22 is in a use state, so that the narrow-band light with different wavelengths, which is emitted by the second light sources 12 alternately, is irradiated on the object to be photographed, so that the object to be photographed generates fluorescence with different colors, and the image pickup main body 21 is configured to capture the object to be photographed under the effect of the narrow-band light with different wavelengths, so as to obtain a plurality of second images, where fluorescence parts in the second images are different colors; and the processor 3 is configured to combine the fluorescent portions of different colors in the first image and the plurality of second images to generate a first combined image.
For example, if the first light source 11 emits white light, the number of the second light sources 12 is three, where the first and second light sources 12 emit excitation light with a wavelength of 525nm, the second and second light sources 12 emit excitation light with a wavelength of 575nm, and the third and second light sources 12 emit excitation light with a wavelength of 610nm, at a first time, the optical filter 22 is in a retracted state, so that visible light emitted by the first and second light sources 11 and 12, respectively, is irradiated on an object to be photographed, and the imaging main body 21 is configured to capture the object to be photographed under the effect of the visible light, so as to obtain a first image. At the second time, when the optical filter 22 is in use, the first second light source 12 emits 525nm narrow-band light to irradiate on the object to be photographed, so that the object to be photographed generates green fluorescence, and the image pickup main body 21 is configured to photograph the object to be photographed under the action of the 525nm narrow-band light, so as to obtain a first second image, where a fluorescence portion in the first second image is green. Then, the first second light source 12 is turned off, the second light source 12 emits 575nm narrow-band light to irradiate on the object to be photographed, so that the object to be photographed generates orange fluorescence, and the main camera body 21 is used for shooting the object to be photographed under the action of the 575nm narrow-band light to obtain a second image, wherein a fluorescence part in the second image is orange. And finally, the second light source 12 is turned off, the third second light source 12 emits 610nm narrow-band light to irradiate on the object to be photographed, so that the object to be photographed generates red fluorescence, and the camera main body 21 is used for correspondingly shooting the object to be photographed under the action of the 610nm narrow-band light to obtain a corresponding third second image, wherein the fluorescence part in the third second image is red. The duration of the second time is three times that of the first time, and the processor 3 is configured to combine the first image, the green fluorescence portion in the first image, the orange fluorescence portion in the second image, and the red fluorescence portion in the third image to generate a first combined image. And the rest is repeated to obtain a plurality of composite images.
As shown in fig. 3, in one embodiment, there is provided an image processing method for fluorescence imaging, the image processing method comprising the steps of:
s100, acquiring a first image shot when the reference light irradiates the object to be shot at a first moment.
The reference light is white light emitted by the first light source, and the first image is shot by the camera device.
And S200, acquiring exciting light to irradiate the object to be shot at a second moment so as to enable the object to be shot to generate a second image shot when fluorescence is generated.
Wherein the exciting light is narrow-band light emitted by the second light source, and the second image is shot by the camera device.
In one embodiment, the second image is processed to obtain a processed second image.
Specifically, the parameters of the fluorescent part in the second image are adjusted so that the fluorescent part in the second image meets the requirements.
Further, the contrast and the brightness of the fluorescent part in the second image are adjusted to meet the requirements.
S300, synthesizing the fluorescent parts in the first image and the second image to generate a first synthesized image.
At the first time, by acquiring a first image captured when the reference light irradiates the subject, the first image can display the entire clear image and the approximate position and boundary of the tumor in the entire clear image. At the second moment, the object to be shot is irradiated by acquiring exciting light, so that a second image shot when the object to be shot generates fluorescence is generated, the second image can display the accurate position and the boundary of a tumor in the whole blurred image, and finally the fluorescence parts in the first image and the second image are synthesized to generate a first synthesized image, wherein the first synthesized image can display the whole sharp image and can display the accurate position and the boundary of the tumor in the whole sharp image, so that the tumor can be completely resected, and the problem of local tumor recurrence is avoided.
In one embodiment, the image processing method further comprises the steps of:
at a third moment, acquiring a third image shot when the reference light irradiates the object to be shot;
at a fourth moment, acquiring exciting light to irradiate the object to be shot so as to enable the object to be shot to generate a fourth image shot when fluorescence is generated;
synthesizing the fluorescent parts in the third image and the fourth image to generate a second synthesized image;
and so on;
at the Nth moment, acquiring an Nth image shot when the reference light irradiates the object to be shot;
at the Nth moment, acquiring exciting light to irradiate the object to be shot so as to enable the object to be shot to generate an Nth image shot when fluorescence is generated;
and synthesizing the fluorescence parts in the Nth image and the Nth image to generate an N/2 th synthesized image.
As shown in fig. 4, in one embodiment, there is provided an image processing method for fluorescence imaging, the image processing method comprising the steps of:
s10, acquiring a first image shot when the reference light irradiates the object to be shot at a first moment;
s20, at a second moment, acquiring a plurality of excitation lights with different wavelengths to alternately irradiate the object to be photographed so as to enable the object to be photographed to generate a plurality of second images photographed when fluorescence with different colors is generated, wherein fluorescence parts in the plurality of second images are different colors;
s30, combining the fluorescent parts with different colors in the first image and the plurality of second images to generate a first combined image.
As shown in fig. 5, in one embodiment, an image processing apparatus 10 for fluorescence imaging is provided, the image processing apparatus 10 including a first image acquisition module 01, a second image acquisition module 02, and a first composite image module 03.
The first image acquiring module 01 is configured to acquire a first image captured when the reference light irradiates the object to be photographed at a first time.
And the second image acquisition module 02 is configured to acquire a second image captured when the object to be photographed is irradiated by the excitation light at a second time so that the object to be photographed generates fluorescence.
A first image combining module 03, configured to combine the fluorescence portions in the first image and the second image to generate a first combined image.
It should be noted that, a specific implementation process of the image processing apparatus according to the embodiment of the present invention is the same as that of the image processing method, and reference may be made to the method embodiment specifically, and details are not described here again.
In an embodiment, a computer-readable storage medium is provided, comprising program code for causing an electronic device to perform the image processing method of any of the above embodiments, when the program product is run on the electronic device.
In one embodiment, an electronic device is provided, comprising a processor and a memory, wherein the memory stores program code that, when executed by the processor, causes the processor to perform the image processing method of any of the above embodiments.
The integrated module, if implemented in the form of a software functional module and sold or used as a separate product, may be stored in a computer readable storage medium. Based on such understanding, the technical solution of the present invention may be embodied in the form of a software product, which is stored in a storage medium and includes instructions for causing a computer device (which may be a personal computer, a server, or a network device) to execute all or part of the steps of the method according to the embodiments of the present invention. And the aforementioned storage medium includes: a U-disk, a removable hard disk, a Read-Only Memory (ROM), a Random Access Memory (RAM), a magnetic disk or an optical disk, and other various media capable of storing program codes.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the scope of the present invention, and all modifications and equivalents of the present invention, which are made by the contents of the present specification and the accompanying drawings, or directly/indirectly applied to other related technical fields, are included in the scope of the present invention.
Claims (10)
1. An image acquisition system for fluorescence imaging, comprising:
a light source assembly comprising a first light source that emits reference light and a second light source that emits excitation light;
the camera shooting device comprises a camera shooting main body and an optical filter movably arranged on the camera shooting main body, wherein at a first moment, the optical filter is in a retracted state so that visible light emitted by the first light source and the second light source respectively irradiates on an object to be shot, and the camera shooting main body is used for shooting the object to be shot under the action of the visible light to obtain a first image; at a second moment, the optical filter is in a use state, so that narrow-band light which is emitted by the first light source and emitted by the second light source and meets conditions is irradiated on an object to be photographed, the object to be photographed generates fluorescence, and the photographing main body is used for photographing the object to be photographed under the action of the narrow-band light to obtain a second image; and
a processor for synthesizing the fluorescent portions in the first and second images to generate a first composite image.
2. An image acquisition system for fluorescence imaging, comprising:
a light source assembly comprising a first light source emitting reference light and a plurality of second light sources emitting excitation light of different wavelengths;
the camera shooting device comprises a camera shooting main body and an optical filter movably arranged on the camera shooting main body, wherein at a first moment, the optical filter is in a retracted state so that visible light emitted by the first light source and the second light source respectively irradiates on an object to be shot, and the camera shooting main body is used for shooting the object to be shot under the action of the visible light to obtain a first image; at a second moment, the optical filter is in a use state, so that narrow-band light which is emitted by the second light sources and meets conditions and has different wavelengths is irradiated on the object to be shot, the object to be shot generates fluorescence with different colors, the shooting main body is used for shooting the object to be shot under the action of the narrow-band light with different wavelengths, and a plurality of second images are obtained, wherein the fluorescence parts in the second images are different colors; and
a processor for combining the fluorescent portions of different colors in the first image and the plurality of second images to generate a first combined image.
3. An image processing method for fluorescence imaging, comprising:
at a first moment, acquiring a first image shot when a reference light irradiates an object to be shot;
at a second moment, acquiring exciting light to irradiate the object to be shot so as to enable the object to be shot to generate a second image shot when fluorescence is generated;
and synthesizing the fluorescent parts in the first image and the second image to generate a first synthesized image.
4. The image processing method according to claim 3, characterized in that the image processing method further comprises:
at a third moment, acquiring a third image shot when the reference light irradiates the object to be shot;
at a fourth moment, acquiring exciting light to irradiate the object to be shot so as to enable the object to be shot to generate a fourth image shot when fluorescence is generated;
synthesizing the fluorescent parts in the third image and the fourth image to generate a second synthesized image;
and so on;
at the Nth moment, acquiring an Nth image shot when the reference light irradiates the object to be shot;
at the Nth moment, acquiring exciting light to irradiate the object to be shot so as to enable the object to be shot to generate an Nth image shot when fluorescence is generated;
and synthesizing the fluorescence parts in the Nth image and the Nth image to generate an N/2 th synthesized image.
5. The image processing method according to claim 3, wherein the second image is processed to obtain a processed second image.
6. The image processing method of claim 5, wherein processing the second image comprises:
and adjusting the parameters of the fluorescent part in the second image so that the fluorescent part in the second image meets the requirements.
7. The image processing method according to claim 6, wherein the contrast and the brightness of the fluorescent portion in the second image are adjusted so that the contrast and the brightness of the fluorescent portion in the second image satisfy requirements.
8. The image processing method according to claim 3, wherein the reference light is white light and the excitation light is narrow-band light.
9. An image processing method for fluorescence imaging, comprising:
at a first moment, acquiring a first image shot when a reference light irradiates an object to be shot;
at a second moment, acquiring a plurality of excitation lights with different wavelengths to alternately irradiate the object to be shot so as to enable the object to be shot to generate a plurality of second images shot when fluorescence with different colors is generated, wherein fluorescence parts in the plurality of second images are different colors;
and synthesizing the fluorescent parts with different colors in the first image and the plurality of second images to generate a first synthesized image.
10. An image processing apparatus for fluorescence imaging, comprising:
the first image acquisition module is used for acquiring a first image shot when the reference light irradiates the object to be shot at a first moment;
the second image acquisition module is used for acquiring exciting light to irradiate the object to be shot at a second moment so as to enable the object to be shot to generate a second image shot when fluorescence is generated;
and the first synthetic image module is used for synthesizing the fluorescence parts in the first image and the second image to generate a first synthetic image.
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