CN113216979B - Microbial reinforcement method for fractured rock mass - Google Patents
Microbial reinforcement method for fractured rock mass Download PDFInfo
- Publication number
- CN113216979B CN113216979B CN202110505587.1A CN202110505587A CN113216979B CN 113216979 B CN113216979 B CN 113216979B CN 202110505587 A CN202110505587 A CN 202110505587A CN 113216979 B CN113216979 B CN 113216979B
- Authority
- CN
- China
- Prior art keywords
- nutrient solution
- rock mass
- fractured rock
- bacillus
- consolidation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000011435 rock Substances 0.000 title claims abstract description 62
- 238000000034 method Methods 0.000 title claims abstract description 19
- 230000002787 reinforcement Effects 0.000 title abstract description 19
- 230000000813 microbial effect Effects 0.000 title description 13
- 235000015097 nutrients Nutrition 0.000 claims abstract description 46
- 244000005700 microbiome Species 0.000 claims abstract description 33
- 238000007596 consolidation process Methods 0.000 claims abstract description 26
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 23
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 14
- 239000001397 quillaja saponaria molina bark Substances 0.000 claims abstract description 13
- 229930182490 saponin Natural products 0.000 claims abstract description 13
- 150000007949 saponins Chemical class 0.000 claims abstract description 13
- QXJDESBAUIILLG-UHFFFAOYSA-N 1,2,3,3,4,4,4-heptafluoro-1-iodobut-1-ene Chemical compound FC(I)=C(F)C(F)(F)C(F)(F)F QXJDESBAUIILLG-UHFFFAOYSA-N 0.000 claims abstract description 9
- HOVAGTYPODGVJG-UHFFFAOYSA-N methyl beta-galactoside Natural products COC1OC(CO)C(O)C(O)C1O HOVAGTYPODGVJG-UHFFFAOYSA-N 0.000 claims abstract description 9
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims abstract description 7
- 239000001888 Peptone Substances 0.000 claims abstract description 7
- 108010080698 Peptones Proteins 0.000 claims abstract description 7
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims abstract description 7
- 239000004202 carbamide Substances 0.000 claims abstract description 7
- 235000019319 peptone Nutrition 0.000 claims abstract description 7
- 239000012137 tryptone Substances 0.000 claims abstract description 7
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 6
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 6
- 241000588986 Alcaligenes Species 0.000 claims abstract description 5
- 241000194108 Bacillus licheniformis Species 0.000 claims abstract description 5
- 244000269722 Thea sinensis Species 0.000 claims abstract 2
- 239000000243 solution Substances 0.000 claims description 63
- 239000012141 concentrate Substances 0.000 claims description 14
- 244000068988 Glycine max Species 0.000 claims description 6
- 235000010469 Glycine max Nutrition 0.000 claims description 6
- 239000002904 solvent Substances 0.000 claims description 6
- 238000002347 injection Methods 0.000 claims description 4
- 239000007924 injection Substances 0.000 claims description 4
- 230000002906 microbiologic effect Effects 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 9
- 230000002308 calcification Effects 0.000 abstract description 4
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 abstract description 2
- 241000193395 Sporosarcina pasteurii Species 0.000 abstract description 2
- 238000010521 absorption reaction Methods 0.000 abstract description 2
- 229910001424 calcium ion Inorganic materials 0.000 abstract description 2
- 241001122767 Theaceae Species 0.000 description 11
- 210000004027 cell Anatomy 0.000 description 7
- 230000000052 comparative effect Effects 0.000 description 7
- 239000000463 material Substances 0.000 description 6
- 239000004575 stone Substances 0.000 description 6
- 238000010276 construction Methods 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- 239000004576 sand Substances 0.000 description 5
- 229910000831 Steel Inorganic materials 0.000 description 4
- 230000003014 reinforcing effect Effects 0.000 description 4
- 239000010959 steel Substances 0.000 description 4
- 230000001580 bacterial effect Effects 0.000 description 3
- 230000033558 biomineral tissue development Effects 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 3
- 239000012535 impurity Substances 0.000 description 3
- 238000002791 soaking Methods 0.000 description 3
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000005057 refrigeration Methods 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 238000007664 blowing Methods 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 239000004568 cement Substances 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- QWPPOHNGKGFGJK-UHFFFAOYSA-N hypochlorous acid Chemical compound ClO QWPPOHNGKGFGJK-UHFFFAOYSA-N 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- HOVAGTYPODGVJG-ZFYZTMLRSA-N methyl alpha-D-glucopyranoside Chemical compound CO[C@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O HOVAGTYPODGVJG-ZFYZTMLRSA-N 0.000 description 1
- 239000004570 mortar (masonry) Substances 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- E—FIXED CONSTRUCTIONS
- E21—EARTH OR ROCK DRILLING; MINING
- E21D—SHAFTS; TUNNELS; GALLERIES; LARGE UNDERGROUND CHAMBERS
- E21D9/00—Tunnels or galleries, with or without linings; Methods or apparatus for making thereof; Layout of tunnels or galleries
- E21D9/001—Improving soil or rock, e.g. by freezing; Injections
- E21D9/002—Injection methods characterised by the chemical composition used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
-
- E—FIXED CONSTRUCTIONS
- E02—HYDRAULIC ENGINEERING; FOUNDATIONS; SOIL SHIFTING
- E02D—FOUNDATIONS; EXCAVATIONS; EMBANKMENTS; UNDERGROUND OR UNDERWATER STRUCTURES
- E02D17/00—Excavations; Bordering of excavations; Making embankments
- E02D17/20—Securing of slopes or inclines
-
- E—FIXED CONSTRUCTIONS
- E02—HYDRAULIC ENGINEERING; FOUNDATIONS; SOIL SHIFTING
- E02D—FOUNDATIONS; EXCAVATIONS; EMBANKMENTS; UNDERGROUND OR UNDERWATER STRUCTURES
- E02D3/00—Improving or preserving soil or rock, e.g. preserving permafrost soil
- E02D3/02—Improving by compacting
- E02D3/10—Improving by compacting by watering, draining, de-aerating or blasting, e.g. by installing sand or wick drains
Landscapes
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Mining & Mineral Resources (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- General Life Sciences & Earth Sciences (AREA)
- General Chemical & Material Sciences (AREA)
- Structural Engineering (AREA)
- Paleontology (AREA)
- Environmental & Geological Engineering (AREA)
- Soil Sciences (AREA)
- Civil Engineering (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agronomy & Crop Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Geochemistry & Mineralogy (AREA)
- Geology (AREA)
Abstract
The invention discloses a microorganism reinforcement method for fractured rock mass, and belongs to the technical field of fractured rock mass reinforcement. The process is as follows: firstly, injecting microorganism strain concentrated solution into fractured rock mass, and then sequentially adding DSMZ nutrient solution and consolidation nutrient solution; wherein the microorganism strain is Bacillus pasteurii, and also can be Bacillus alcaligenes, bacillus licheniformis or Bacillus subtilis, and the concretion nutrient solution comprises the following components: tryptone, soyase peptone, anhydrous calcium chloride, urea, methyl-alpha-D-glucopyranoside, tea saponin and water. The consolidation nutrient solution disclosed by the invention can enhance the absorption effect of bacillus on calcium ions and promote calcification, so that an excellent cementation effect of fractured rock mass is realized.
Description
Technical Field
The invention relates to the technical field of fractured rock mass reinforcement, in particular to a microorganism reinforcement method for fractured rock mass.
Background
The development of the river valley slope mountain body fissure rock mass in the plateau area increases with the increase of the elevation. Under natural conditions and construction interference, the local rock blocks of the fractured rock mass can collapse at any time, and on high and steep mountain bodies, falling rocks form a great threat to engineering construction roads, personnel and equipment safety, and the risk is at any time and is ubiquitous in the whole mountain bodies on two sides, so that the fractured rock mass needs to be reinforced and treated.
The traditional method has the defects of extremely large engineering quantity, extremely long construction period, unacceptable manufacturing cost and unsatisfactory reinforcement effect when the broken fractured rock mass spread over the mountain bodies on two sides is reinforced and treated. In order to solve the problem, researchers reinforce the fractured rock mass by adopting a microorganism reinforcement technology, and compared with the traditional fractured rock mass reinforcement mode, the fractured rock mass is cemented by utilizing the microorganism mineralization, so that the integrity and strength of the rock can be enhanced, and meanwhile, the method has the characteristics of strong operability and environmental friendliness, and has extremely high application value.
Although the method has good application prospect in microbial reinforcement of fractured rock mass, the bonding effect of the existing microbial reinforcement method on the fractured rock mass is still not ideal.
Disclosure of Invention
The invention aims to provide a microorganism reinforcement method for fractured rock mass, which solves the problems in the prior art, thereby remarkably improving the bonding effect of microorganism reinforcement on the fractured rock mass.
In order to achieve the above object, the present invention provides the following solutions:
the invention aims at providing a consolidation nutrient solution, which comprises the following components in percentage by weight: 9-11g/L of tryptone, 2-3g/L of soybean peptone, 48-50g/L of anhydrous calcium chloride, 33-35g/L of urea, 8-10g/L of methyl-alpha-D-glucopyranoside and 0.4-0.5g/L of tea saponin, wherein the solvent is water.
The second object of the invention is to provide a microorganism reinforcement method for fractured rock mass, comprising the following steps:
injecting the microorganism strain concentrated solution into the fractured rock mass, and then sequentially adding the DSMZ nutrient solution and the consolidation nutrient solution;
the microorganism strain concentrated solution is any concentrated solution of bacillus barbitarus, bacillus alcaligenes, bacillus licheniformis or bacillus subtilis; the density of the microorganism strain concentrate is 2.0-2.6X10 9 cells/ml；
The consolidation nutrient solution is the consolidation nutrient solution.
Further, the volume ratio of the microorganism strain concentrated solution, the DSMZ nutrient solution and the consolidation nutrient solution is 1:8-10:5-6.
Further, the microorganism strain concentrated solution, the DSMZ nutrient solution and the consolidation nutrient solution are injected into the fractured rock mass in an auxiliary way by adopting an injection pipe.
The invention further aims to provide an application of the consolidated nutrient solution in solidification of fractured rock mass.
Further, the application method comprises the following steps:
injecting the microorganism strain concentrated solution into the fractured rock mass, and then sequentially adding the DSMZ nutrient solution and the consolidation nutrient solution;
the microorganism strain concentrated solution is any concentrated solution of bacillus barbitarus, bacillus alcaligenes, bacillus licheniformis or bacillus subtilis; the density of the microorganism strain concentrate is 2.0-2.6X10 9 cells/ml. The invention discloses the following technical effects:
as the tea saponin has affinity to cell membranes, the sterilization and bacteriostasis effects of the tea saponin are commonly utilized in the prior art. According to the invention, the methyl-alpha-D-glucopyranoside and the tea saponin are added into the nutrient solution, and the fact that under the condition that the methyl-alpha-D-glucopyranoside exists, the trace addition of the tea saponin is controlled can promote the absorption of bacillus to calcium ions and promote calcification, so that the cementation effect is obviously enhanced.
The invention fully utilizes the natural mineralization of microorganisms to realize restoration, does not release toxic and harmful gases, meets the requirements on ecological balance and environmental friendliness, avoids the input of a large amount of cement grouting materials or chemical grouting materials, and has important guiding significance on the green treatment and protection of dangerous rock bodies.
The invention aims at reinforcing the fractured rock mass and can be applied to reinforcing and treating engineering side slopes such as hydropower engineering side slopes, road engineering side slopes, railway side slopes and the like; reinforcing and treating surrounding rocks of a diversion tunnel, a traffic tunnel and an oil reserve tunnel warehouse; treatment of dangerous rock mass in scenic spots; and the fields of port channel engineering construction, sea reclamation, island reef development, desert control, sand storm control and the like.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic illustration of the application of the bio-reinforcement approach of the present invention in engineering;
FIG. 2 is a schematic diagram of the principles of bio-augmentation calcification of the present invention;
FIG. 3 is a fractured rock sample prior to microbial consolidation of example 1;
FIG. 4 is a microbiologically consolidated cemented rock sample of example 1.
Detailed Description
Various exemplary embodiments of the invention will now be described in detail, which should not be considered as limiting the invention, but rather as more detailed descriptions of certain aspects, features and embodiments of the invention.
It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. In addition, for numerical ranges in this disclosure, it is understood that each intermediate value between the upper and lower limits of the ranges is also specifically disclosed. Every smaller range between any stated value or stated range, and any other stated value or intermediate value within the stated range, is also encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.
Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference for the purpose of disclosing and describing the methods and/or materials associated with the documents. In case of conflict with any incorporated document, the present specification will control.
It will be apparent to those skilled in the art that various modifications and variations can be made in the specific embodiments of the invention described herein without departing from the scope or spirit of the invention. Other embodiments will be apparent to those skilled in the art from consideration of the specification of the present invention. The specification and examples of the present invention are exemplary only.
As used herein, the terms "comprising," "including," "having," "containing," and the like are intended to be inclusive and mean an inclusion, but not limited to.
The invention discloses a method for reinforcing fractured rock mass by microorganisms, which comprises the following steps in engineering implementation:
1. and selecting a range of the fractured rock mass to be reinforced according to factors such as engineering importance influence, slope rock mass structural stability and the like.
2. In the selected range, grouting steel flowtube (the diameter is selected to be 25mm, 32mm, 48mm, 60mm and the like according to the crack size) is inserted according to the row spacing and the row spacing of about 2m, and then sand soil and other materials are adopted to fill the cracks of the crack rock body.
3. Preparing a microorganism strain concentrated solution: resuscitating the cryopreserved strain, and preparing into bacterial density of 2.0-2.6X10 with DSMZ culture solution 9 Placing the concentrated solution of the microbial strain in cells/ml in an environment of 4 ℃ for refrigeration for later use;
the strain is one of Bacillus pasteurii, bacillus alcaligenes, bacillus licheniformis or Bacillus subtilis;
wherein, the preparation steps of the bacillus pasteurizer concentrated solution are as follows: firstly, resuscitating and culturing the bacillus pasteurizer strain adsorbed by ceramic beads and stored in a refrigerator at the temperature of minus 80 ℃, then injecting the bacillus pasteurizer strain into a container which is pre-sterilized by high temperature and is filled with DSMZ culture solution, placing the container in a constant temperature shaking table, keeping the temperature of 30 ℃ for 10 hours, and taking out the container. Transferring to high-speed centrifuge for separation (rotation speed of 6000 rpm), pouring off excessive liquid, and blowing with pipettor to obtain Bacillus pasteurizer concentrate and bacterial densityThe degree is 2.0-2.6X10 9 The cells/ml are placed in an environment of 4 ℃ for refrigeration for standby.
4. Preparing a consolidation nutrient solution: the formula of the consolidation nutrient solution is as follows: 9-11g/L of tryptone, 2-3g/L of soybean peptone, 48-50g/L of anhydrous calcium chloride, 33-35g/L of urea, 8-10g/L of methyl-alpha-D-glucopyranoside and 0.4-0.5g/L of tea saponin, wherein the solvent is water. In order to reduce the application cost and facilitate site construction, the water is prepared by using a site water source, and the water source is selected to be clean as much as possible and used after being filtered. Ordinary tap water is not suitable as a water source because residual hypochlorous acid therein has an inhibitory effect on microorganisms.
5. Firstly, filling the microorganism strain concentrate prepared in the step 3 into a steel flower pipe, and then filling DSMZ nutrient solution with the volume of 8-10 times of the microorganism strain concentrate. The main purpose of this step is to activate the biological activities of the bacillus pasteurizer and the indigenous microorganisms of the sand as soon as possible and to introduce the bacillus pasteurizer into the cracks of the sand and rock mass by means of the pressure difference created by the perfused nutrient solution. When the nutrient solution is poured, a principle of a small amount of repeated use is adopted, a certain pouring pressure is ensured, and the adding amount of the DSMZ nutrient solution is gradually reduced each time until the pouring is completed. If the concentration of the bacillus pasteurizer is insufficient, the bacillus pasteurizer can be added in a supplementing way at any time in the later period.
6. Injecting the prepared concretion nutrient solution: after the DSMZ nutrient solution is poured, the concentrated microorganism strain solution is continuously poured into the solidified nutrient solution with the volume of 5-6 times. The injection adopts the principle of a small amount for many times, and the adding amount of the consolidation nutrient solution is gradually reduced each time until the injection is completed. The prepared concretion nutrient solution should be used as soon as possible so as to avoid the influence of the deterioration of the components.
7. Sampling and detecting the filling condition of the microbial mineralization products on the gaps of the side slope of the accumulation body, wherein the filling condition is not ideal (such as sand and rock and fissure are not bonded together or the cementation amount of the fissure is less than half area), and new holes are arranged at the periphery of the gap and are filled again.
8. And (5) drying the solution in the steel floral tube to be poured, pouring concrete mortar, and sealing the grouting steel floral tube.
9. And (5) sampling and checking to be qualified, and finishing the reinforcement of the fractured rock mass.
FIG. 1 is a schematic illustration of the application of the bio-reinforcement approach of the present invention in engineering; FIG. 2 is a schematic diagram of the principles of bio-augmentation calcification of the present invention.
Taking the Meiyan rock as an example, the reinforcement effect of the microorganism fractured rock mass is compared.
In the following examples, metandine fractured rock samples were processed into cylindrical fractured rock samples having a height of 50mm and a diameter of 50mm.
Example 1:
the concretion nutrient solution in the embodiment comprises the following components:
9g/L of tryptone, 2g/L of soybean peptone, 50g/L of anhydrous calcium chloride, 34g/L of urea, 8g/L of methyl-alpha-D-glucopyranoside and 0.5g/L of tea saponin, and the solvent is water.
Microbial consolidation of fractured rock mass:
(1) Soaking the fractured rock sample in distilled water for 24 hours, and removing impurities on the surface of the sample;
(2) After aligning two pieces of the fractured rock sample, pushing the two pieces into a sleeve with the inner diameter of 50mm, filling a permeable stone at the bottom of the sample, keeping the permeable stone level with the bottom of the sleeve, and arranging a transfusion needle and a dropper at the top of the sleeve;
(3) 15mL of Bacillus pasteurisus concentrate (2.6X10 strain concentration) was added to the cartridge at a rate of 0.50mL/min 9 cells/ml)；
(4) After the liquid drop of the bacillus barbituralis concentrate is completed, 150mL of DSMZ nutrient solution is added into the sleeve at the rate of 2mL/min, and the sleeve is left stand for 0.5h after the addition is completed;
(5) After the standing is completed, 90mL of consolidation nutrient solution is added into the sleeve at the rate of 1mL/min, and after the dripping is completed, the standing is carried out for 0.5h, thus completing the microbial reinforcement of the fractured rock mass.
Fig. 3 shows a fractured rock sample before microorganism consolidation in this example, and fig. 4 shows a cemented rock sample after microorganism consolidation.
Example 2
The concretion nutrient solution in the embodiment comprises the following components:
11g/L of tryptone, 3g/L of soybean peptone, 48g/L of anhydrous calcium chloride, 35g/L of urea, 9g/L of methyl-alpha-D-glucopyranoside and 0.4g/L of tea saponin, and the solvent is water.
Microbial consolidation of fractured rock mass:
(1) Soaking the fractured rock sample in distilled water for 24 hours, and removing impurities on the surface of the sample;
(2) After aligning two pieces of the fractured rock sample, pushing the two pieces into a sleeve with the inner diameter of 50mm, filling a permeable stone at the bottom of the sample, keeping the permeable stone level with the bottom of the sleeve, and arranging a transfusion needle and a dropper at the top of the sleeve;
(3) 15mL of Bacillus pasteurisus concentrate (2.0X10 strain concentration) was added to the cartridge at a rate of 0.50mL/min 9 cells/ml)；
(4) After the liquid drop of the bacillus barbituralis concentrate is completed, adding 120mL of DSMZ nutrient solution into the sleeve at the rate of 2mL/min, and standing for 0.5h after the addition is completed;
(5) After the standing is completed, 75mL of consolidation nutrient solution is added into the sleeve at the rate of 1mL/min, and after the dripping is completed, the standing is carried out for 0.5h, thus completing the microbial reinforcement of the fractured rock mass.
Example 3
The concretion nutrient solution in the embodiment comprises the following components:
10g/L of tryptone, 2g/L of soybean peptone, 49g/L of anhydrous calcium chloride, 33g/L of urea, 10g/L of methyl-alpha-D-glucopyranoside, 0.5g/L of tea saponin and water as a solvent.
Microbial consolidation of fractured rock mass:
(1) Soaking the fractured rock sample in distilled water for 24 hours, and removing impurities on the surface of the sample;
(2) After aligning two pieces of the fractured rock sample, pushing the two pieces into a sleeve with the inner diameter of 50mm, filling a permeable stone at the bottom of the sample, keeping the permeable stone level with the bottom of the sleeve, and arranging a transfusion needle and a dropper at the top of the sleeve;
(3) 15mL of the Bacillus subtilis concentrate (bacterial liquid concentration: 2.4X10) was added to the cartridge at a rate of 0.50mL/min 9 cells/ml)；
(4) After the liquid drop of the bacillus barbituralis concentrate is completed, 140mL of DSMZ nutrient solution is added into the sleeve at the rate of 2mL/min, and the sleeve is left stand for 0.5h after the addition is completed;
(5) After the standing is completed, 80mL of consolidation nutrient solution is added into the sleeve at the rate of 1mL/min, and after the dripping is completed, the standing is carried out for 0.5h, thus completing the microbial reinforcement of the fractured rock mass.
Comparative example 1
The difference from example 1 is only that no methyl-alpha-D-glucopyranose is added.
Comparative example 2
The only difference from example 1 is that no tea saponin was added.
Comparative example 3
The difference from example 1 is only that the content of tea saponin is 1g/L.
The data on the properties of the fractured rock mass after strengthening of examples 1-3 and comparative examples 1-3 are shown in Table 1:
TABLE 1
| Uniaxial compressive strength/MPa | Calcium carbonate content/% | Permeability coefficient | |
| Example 1 | 6.8 | 34.29 | (3.2-4.5)×10 -5 cm/s |
| Example 2 | 6.7 | 33.56 | (3.6-4.6)×10 -5 cm/s |
| Example 3 | 6.8 | 33.45 | (3.7-4.7)×10 -5 cm/s |
| Comparative example 1 | 2.1 | 9.21 | (0.3-2.1)×10 -4 cm/s |
| Comparative example 2 | 3.2 | 12.36 | (0.3-1.9)×10 -4 cm/s |
| Comparative example 3 | 2.9 | 10.42 | (0.2-1.8)×10 -4 cm/s |
The above embodiments are only illustrative of the preferred embodiments of the present invention and are not intended to limit the scope of the present invention, and various modifications and improvements made by those skilled in the art to the technical solutions of the present invention should fall within the protection scope defined by the claims of the present invention without departing from the design spirit of the present invention.
Claims (4)
1. The consolidation nutrient solution is characterized by comprising the following components in percentage by weight: 9-11g/L of tryptone, 2-3g/L of soybean peptone, 48-50g/L of anhydrous calcium chloride, 33-35g/L of urea, 8-10g/L of methyl-alpha-D-glucopyranoside and 0.4-0.5g/L of tea saponin, wherein the solvent is water.
2. A method of microbiological consolidation of a fractured rock mass comprising the steps of:
injecting the microorganism strain concentrated solution into the fractured rock mass, and then sequentially adding the DSMZ nutrient solution and the consolidation nutrient solution;
the microorganism strain concentrated solution is any concentrated solution of bacillus barbitarus, bacillus alcaligenes, bacillus licheniformis or bacillus subtilis; the density of the microorganism strain concentrate is 2.0-2.6X10 9 cells/mL；
The consolidated nutrient solution is the consolidated nutrient solution of claim 1.
3. The method of claim 2, wherein the volume ratio of the microorganism strain concentrate, the DSMZ nutrient solution, and the consolidation nutrient solution is 1:8-10:5-6.
4. The method of claim 2, wherein the microorganism strain concentrate, the DSMZ nutrient solution, and the consolidation nutrient solution are injected into the fractured rock mass with the aid of an injection tube.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110505587.1A CN113216979B (en) | 2021-05-10 | 2021-05-10 | Microbial reinforcement method for fractured rock mass |
| NL2031188A NL2031188B1 (en) | 2021-05-10 | 2022-03-08 | Microbial reinforcement method of fractured rock masses |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110505587.1A CN113216979B (en) | 2021-05-10 | 2021-05-10 | Microbial reinforcement method for fractured rock mass |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113216979A CN113216979A (en) | 2021-08-06 |
| CN113216979B true CN113216979B (en) | 2024-03-08 |
Family
ID=77094208
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110505587.1A Active CN113216979B (en) | 2021-05-10 | 2021-05-10 | Microbial reinforcement method for fractured rock mass |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN113216979B (en) |
| NL (1) | NL2031188B1 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113585301B (en) * | 2021-09-02 | 2022-05-17 | 南京大学 | Method for treating rock slope collapse by using microbial film and MICP (micro-emulsion-phase phosphate) technology |
Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0787971A (en) * | 1991-12-10 | 1995-04-04 | Kao Corp | Calboxymethyl cellulase and microorganism producing the enzyme |
| JP2001238673A (en) * | 2000-03-03 | 2001-09-04 | Fuji Chemical Industries Ltd | Preparation method for immobilized glycosidation enzyme and glucoside |
| WO2008002971A2 (en) * | 2006-06-29 | 2008-01-03 | Microbia, Inc. | Methods and compositions for the treatment of gastrointestinal disorders |
| CN102603849A (en) * | 2012-02-10 | 2012-07-25 | 厦门华侨亚热带植物引种园 | Gynostemma pentaphylla secondary saponin, preparation method and applications thereof |
| CN104556896A (en) * | 2014-12-19 | 2015-04-29 | 安徽中龙建材科技有限公司 | High-strength aerocrete building block and preparation method thereof |
| EP2988746A1 (en) * | 2013-04-23 | 2016-03-02 | Merck Sharp & Dohme Corp. | Halo and trifluoromethyl substituted orexin receptor antagonists |
| WO2016159580A1 (en) * | 2015-03-31 | 2016-10-06 | (주)아모레퍼시픽 | Composition containing theasapogenol derivative as active ingredient |
| CN107675675A (en) * | 2017-08-29 | 2018-02-09 | 华能澜沧江水电股份有限公司 | A kind of accumulation body slope microorganism reinforcement means |
| CN107677527A (en) * | 2017-08-29 | 2018-02-09 | 华能澜沧江水电股份有限公司 | A kind of rock triaxial test destroys test micro reinforcement means |
| CN109516713A (en) * | 2018-10-29 | 2019-03-26 | 俞小峰 | A kind of lightweight concrete foaming agent and preparation method thereof |
| AU2020103285A4 (en) * | 2020-11-06 | 2021-01-14 | Shenzhen University | A method for improving the properties of recycled concrete aggregates by using bacillus h4 |
| CN112239672A (en) * | 2020-09-29 | 2021-01-19 | 哈尔滨工大岩土工程技术有限公司 | Microbial reinforcement liquid for slope reinforcement, preparation method and construction method thereof |
| CN112759491A (en) * | 2021-03-05 | 2021-05-07 | 中国水稻研究所 | Preparation method of saline-alkali soil conditioner |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7713942B2 (en) * | 2001-04-04 | 2010-05-11 | Nordic Vaccine Technology A/S | Cage-like microparticle complexes comprising sterols and saponins for delivery of polynucleotides |
| US8182604B2 (en) * | 2004-12-20 | 2012-05-22 | Murdoch University | Microbial biocementation |
| US8183184B2 (en) * | 2006-09-05 | 2012-05-22 | University Of Kansas | Polyelectrolyte complexes for oil and gas applications |
| US9199880B2 (en) * | 2010-04-27 | 2015-12-01 | Biomason, Inc. | Methods for making construction materials using enzyme producing bacteria |
| US20180194697A1 (en) * | 2017-01-12 | 2018-07-12 | Khanh Le | Microbial soil enhancements |
-
2021
- 2021-05-10 CN CN202110505587.1A patent/CN113216979B/en active Active
-
2022
- 2022-03-08 NL NL2031188A patent/NL2031188B1/en active
Patent Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0787971A (en) * | 1991-12-10 | 1995-04-04 | Kao Corp | Calboxymethyl cellulase and microorganism producing the enzyme |
| JP2001238673A (en) * | 2000-03-03 | 2001-09-04 | Fuji Chemical Industries Ltd | Preparation method for immobilized glycosidation enzyme and glucoside |
| WO2008002971A2 (en) * | 2006-06-29 | 2008-01-03 | Microbia, Inc. | Methods and compositions for the treatment of gastrointestinal disorders |
| CN102603849A (en) * | 2012-02-10 | 2012-07-25 | 厦门华侨亚热带植物引种园 | Gynostemma pentaphylla secondary saponin, preparation method and applications thereof |
| EP2988746A1 (en) * | 2013-04-23 | 2016-03-02 | Merck Sharp & Dohme Corp. | Halo and trifluoromethyl substituted orexin receptor antagonists |
| CN104556896A (en) * | 2014-12-19 | 2015-04-29 | 安徽中龙建材科技有限公司 | High-strength aerocrete building block and preparation method thereof |
| WO2016159580A1 (en) * | 2015-03-31 | 2016-10-06 | (주)아모레퍼시픽 | Composition containing theasapogenol derivative as active ingredient |
| CN107675675A (en) * | 2017-08-29 | 2018-02-09 | 华能澜沧江水电股份有限公司 | A kind of accumulation body slope microorganism reinforcement means |
| CN107677527A (en) * | 2017-08-29 | 2018-02-09 | 华能澜沧江水电股份有限公司 | A kind of rock triaxial test destroys test micro reinforcement means |
| CN109516713A (en) * | 2018-10-29 | 2019-03-26 | 俞小峰 | A kind of lightweight concrete foaming agent and preparation method thereof |
| CN112239672A (en) * | 2020-09-29 | 2021-01-19 | 哈尔滨工大岩土工程技术有限公司 | Microbial reinforcement liquid for slope reinforcement, preparation method and construction method thereof |
| AU2020103285A4 (en) * | 2020-11-06 | 2021-01-14 | Shenzhen University | A method for improving the properties of recycled concrete aggregates by using bacillus h4 |
| CN112759491A (en) * | 2021-03-05 | 2021-05-07 | 中国水稻研究所 | Preparation method of saline-alkali soil conditioner |
Non-Patent Citations (3)
| Title |
|---|
| Zhiliang Yu等 ; .Study on the antifungal activity and mechanism of tea saponin from Camellia oleifera cake.European Food Research and Technology.2020,全文. * |
| 季铵盐改性茶皂素的合成与性能研究;马戎;戴玉梅;;日用化学工业(03);全文 * |
| 酶解辅助提取茶皂苷的工艺参数研究;韩敏;;食品科技(04);全文 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113216979A (en) | 2021-08-06 |
| NL2031188B1 (en) | 2023-01-23 |
| NL2031188A (en) | 2022-11-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Sun et al. | Improvement of microbial-induced calcium carbonate precipitation technology for sand solidification | |
| Jiang et al. | Applicability of microbial calcification method for sandy-slope surface erosion control | |
| Zhang et al. | Microbial‑induced carbonate precipitation (MICP) technology: a review on the fundamentals and engineering applications | |
| El Mountassir et al. | Applications of microbial processes in geotechnical engineering | |
| Nemati et al. | Modification of porous media permeability, using calcium carbonate produced enzymatically in situ | |
| Van Paassen | Bio-mediated ground improvement: from laboratory experiment to pilot applications | |
| Shahrokhi-Shahraki et al. | Improving sand with microbial-induced carbonate precipitation | |
| Sharma et al. | Rock-like behavior of biocemented sand treated under non-sterile environment and various treatment conditions | |
| Rong et al. | Influence of molding process on mechanical properties of sandstone cemented by microbe cement | |
| Khan et al. | Coral sand solidification test based on microbially induced carbonate precipitation using ureolytic bacteria | |
| CA2725708C (en) | Biotechnological process for hydrocarbon recovery in low permeability porous media | |
| CN113216979B (en) | Microbial reinforcement method for fractured rock mass | |
| Zhong et al. | A new microbial plugging process and its impact on fracture remediation | |
| KR101380354B1 (en) | Composition for improvement of soft ground and bio-grout method using the same | |
| Gui et al. | Experimental Study on the Fine‐Grained Uranium Tailings Reinforced by MICP | |
| CN105837370A (en) | An environmental friendly soil ecology modifying agent and a preparing method thereof | |
| CN108863212B (en) | Self-purification vegetation type permeable slope protection material and preparation method thereof | |
| Ivanov et al. | Bioclogging and biogrouts | |
| CN214895279U (en) | Test device for simulating earthen site cracks through microorganism grouting and curing | |
| CN212714817U (en) | Artifical lake bottom concrete crack repair system | |
| Soundara et al. | A critical review on soil stabilization using bacteria | |
| Guo et al. | Experimental study on improving hydraulic characteristics of sand via microbially induced calcium carbonate precipitation | |
| CN110340134B (en) | Method for in-situ filling and restoring polluted soil | |
| CN114804732B (en) | Method for preparing roadbed filler by using alkaline residue and microorganism modified tailing sand | |
| Kou et al. | Comparative Study on Strength and Permeability of Siliceous Sand Treated by MICP and Cement Grouting. |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |