CN113215109A

CN113215109A - Construction of African swine fever polygene combined deletion attenuated strain and application of attenuated strain as vaccine

Info

Publication number: CN113215109A
Application number: CN202110450549.0A
Authority: CN
Inventors: 郑海学; 李丹; 党文; 张克山; 刘华南; 申超超; 冯涛; 李攀; 齐晓兰; 张敬; 刘迎琦; �田宏; 茹毅; 杨帆; 曹伟军; 何继军; 刘志杰; 张伟; 郭建宏; 殷宏
Original assignee: Lanzhou Veterinary Research Institute of CAAS
Current assignee: Lanzhou Veterinary Research Institute of CAAS
Priority date: 2020-07-10
Filing date: 2021-04-25
Publication date: 2021-08-06
Anticipated expiration: 2041-04-25
Also published as: CN112063592A; CN113215109B

Abstract

The invention belongs to the technical field of biological engineering, and particularly relates to an attenuated African swine fever virus with a deleted immune suppressor gene and a vaccine. The invention firstly discovers that ASFV MGF-110-9L gene and ASFV MGF-505-7R gene have stronger immune suppression effect, and the gene is knocked out on African swine fever virus to weaken the virulence of the African swine fever virus, thus being a new target gene for preparing attenuated swine fever vaccine; the function of the ASFV G-ACD-00190 gene, the ASFV MGF-110-9L gene, the ASFV G-ACD-00210 gene and the ASFV MGF-505-7R gene coding protein is jointly deleted in the African swine fever parent strain ASFV CN/GS/2018 by adopting a genetic engineering means, so that the toxicity of the parent strain is reduced, and a strain is obtainedAttenuated African swine fever candidate vaccine strains with high strain safety; after the attenuated African swine fever candidate vaccine strain is used for immunizing pigs, the pigs are completely weakened and the immunized pigs are healthy, and the attenuated African swine fever candidate vaccine strain can challenge the ASFV CN/GS/2018 virulent strain (100 HAD)₅₀Dosage) provides 100 percent of immune protection, can be used as a safe and effective candidate vaccine for preventing and controlling the epidemic situation of the African swine fever, and has great social value.

Description

Construction of African swine fever polygene combined deletion attenuated strain and application of attenuated strain as vaccine

The present application claims priority from a prior application entitled "construction of a multiple gene-linked deletion attenuated strain for African swine fever and use as a vaccine" filed on 10/7/2020 and having application number CN 2020106621078, the entire contents of which are incorporated herein by reference.

Technical Field

The invention belongs to the technical field of bioengineering, and particularly relates to construction of an African swine fever polygene combined deletion attenuated strain and application of the attenuated strain as a vaccine.

Background

African Swine Fever (ASF) is an acute virulent infectious disease characterized by Fever of pigs and organ bleeding of the whole body of pigs caused by African Swine Fever Virus (ASFV), and the death rate of domestic pigs is as high as 100%.

The current preparation of vaccines for african swine fever is mainly done in three ways: firstly, directly inactivating original African swine fever viruses to obtain inactivated vaccines; secondly, screening out virus proteins which effectively induce immune response, and further preparing subunit vaccines and live vector vaccines; thirdly, a gene deletion means is adopted to knock out virulence genes to obtain the recombinant virus vaccine. The first method is the most common and direct method for preparing virus vaccine, but because the African swine fever virus codes proteins such as immunosuppression, immune tolerance and Antibody Dependence Enhancement (ADE), the inactivated vaccine cannot provide effective immune protection in the immunized pig; in the second aspect, the structure of the African swine fever virus is complex, the protein inducing immune response is not clear at present, and the obtained antigen is not enough to induce strong immune protection. The vaccine which is most expected to break through at present is a gene knockout vaccine, and a vaccine candidate strain which has immunogenicity, can reduce pathogenicity and has an immune protection effect on an immune pig is obtained by knocking out virulence related genes such as immunosuppression, tolerance, ADE and the like. For example, chinese patent CN110551695A provides a four-gene-deleted low virulent strain of african swine fever virus, which is a four-gene-deleted low virulent strain of SY18 isolate of african swine fever virus, and which lacks functional proteins of the following genes: CD2v gene coding products and three polygene family genes (MGF-360-12L, MGF-360-13L, MGF-360-14L) coding products, after the piglets are inoculated for 28 days, the piglets are immunized and then subjected to a virus attack test by using ASFV original virus, and as a result, the immunized pigs are completely protected; for example, Chinese patent CN110093324B discloses a gene-II African swine fever virus MGF-360-505R deletion and CD2V and MGF-360-505R combined deletion gene deletion virus, both strains can provide 100% immune protection for African swine fever Chinese epidemic virulent strains, and the two strains can provide sufficient virulent attack protection 21 days after the immunization of the gene deletion virus.

In summary, the knockout of the virulence gene of classical swine fever virus usually needs to consider immune response and protection to pigs, and also needs to consider pathogenicity and safety. However, the hog cholera virus virulence gene knockout vaccines of the prior art also consider the following factors: (1) whether the knocked-out virulence genes reduce the toxicity and the toxin, whether the knocked-out virulence genes bring tissue damage, influence the production performance and the like is an important index of biological safety and is also an important index of continuous improvement of the safety of gene-deletion vaccines; (2) different strains lack the same gene to produce different effects, lack and have toxicity and pathogenicity to remain the question; and the virus titer caused by polygene deletion is low, so that the risk of reducing immunogenicity or protecting effect and the like of attenuated strains is also caused; (3) although the whole genome sequencing work of the African swine fever is already completed, the number of the regulatory genes and the structural genes which form the African swine fever virus is more than 160, and the engineering is huge, but the functions of the regulatory genes and the structural genes are important for the pathogenesis and the vaccine development of the African swine fever virus.

Therefore, in the preparation process of the attenuated vaccine for African swine fever, the gene function and the influence of the gene function on pathogenicity and immune response are identified, a basis is provided for the selection of a knockout target gene and the construction of gene knockout, the screening of a new target gene is very important, and the safety and the immune effectiveness of a vaccine candidate strain are determined. The above-mentioned patent knockout target genes are all from published literature. The invention discovers for the first time that ASFV MGF-110-9L and ASFV MGF-505-7R genes have stronger immunosuppressive action in the research of gene function and regulation mechanism thereof, and are newly discovered new target genes; according to the invention, through the loss of functions of newly identified target genes ASF V MGF-110-9L gene and ASFV MGF-505-7R gene encoding protein and the loss of functions of ASFV G-ACD-00190 gene and ASFV G-ACD-00210 gene encoding protein which are repeated at two ends of ASFV MGF-110-9L gene, the pathogenicity of the virus is further reduced, the safety of the virus is improved, and a virus strain which is completely weakened to pigs is obtained; after the African swine fever attenuated vaccine is used for immunizing pigs, pigs living with the immunized pigs are not horizontally infected, the immunized pigs are anatomically observed, various tissues and organs are healthy, and further nucleic acid is detected to be negative, and data shows that the attenuated African swine fever virus strain with good safety is obtained through the combined deletion. The virus attacking experiment shows that the attenuated African swine fever virus can have 100% immune protection on the strong strain virus attack separated from ASFV CN/GS/2018, after the virus attack by the ASFV CN/GS/2018 separated strain, the death rate of the attenuated strain immune pig is 0, the immune pig tissue is healthy and has no damage after the virus attack by dissection and nucleic acid detection, so the attenuated African swine fever virus can be used as a safe and effective vaccine for preventing and controlling the epidemic situation of the Chinese African swine fever, and has great social value.

Disclosure of Invention

Aiming at the technical problems, the invention provides a strategy for constructing an African swine fever attenuated strain, firstly, the invention discovers that the gene A SFV MGF-110-9L and the gene ASFV MGF-505-7R have stronger immunosuppressive action and are newly discovered target genes for weakening the African swine fever virus. Based on the above, the invention aims to provide a strategy for constructing an African swine fever attenuated strain by jointly deleting the functions of the ASFV MGF-110-9L gene and the ASFV MGF-505-7R gene in the African swine fever virus. Because the ASFV G-ACD-00190 gene and the ASFV G-ACD-00210 gene are positioned at both ends of the ASFV MGF-110-9L gene and partially overlap with the ASFV MGF-110-9L gene (a partial sequence and a promoter sequence of CDS), the loss of the function of the encoded protein can be caused by the deletion of the whole nucleotide sequence of the ASFV G-ACD-00190 gene, the ASFV G-ACD-00210 gene and the ASFV MGF-110-9L gene, or the loss of the whole nucleotide sequence of the ASFV G-110-9L gene and the deletion of the partial sequence of the ASFV G-ACD-00190 gene and the ASFV G-ACD-00210 gene, the loss of the function of the encoded protein can be caused by the deletion of the whole nucleotide sequence of the ASFV G-ACD-00190 gene, the ASFV MGF-110-9L gene, The proteins coded by the ASFV G-ACD-00210 gene and the ASFV MG F-505-7R gene can not be normally expressed, so that the functions of the coded proteins are lost. According to the common knowledge of those skilled in the art, in addition to the above-mentioned gene editing means, other gene editing means may be used to simultaneously lose the functions of the proteins encoded by the ASFV MGF-505-7R gene, the ASFV G-ACD-00190 gene, the ASFV G-ACD-00210 gene, and the ASFV MGF-110-9L gene, for example: frame shift mutations, point mutations, frame shift deletions, insertion of nucleotide sequences, and the like. The strategy is realized by the following technical scheme:

in a first aspect, the invention provides an application of preparing an attenuated African swine fever virus strain or an African swine fever vaccine by jointly losing functions of proteins encoded by an ASFV G-ACD-00190 gene, an ASFV MGF-110-9L gene, an ASFV G-ACD-00210 gene and an ASFV MGF-505-7R gene in a genotype II African swine fever virus.

Preferably, the genotype II African swine fever virus comprises ASFV CN/GS/2018 isolate, China/2018/Anhui XCGQ, Pig/CN/HLJ/2018, Georgia2007, ASFV-SY18, ASFV/POL/2015/Podlaskie, ASFV/POL17_05838_ C220, ASFV/POL16_29413-o23, ASFV/POL16_20538_ o9, ASFV/POL17_04461_ C210, ASFV/POL16_20186_ o7, any one of ASFV/Pol17_03029_ C201, ASFV/Pol16_20540_ o10, ASFV Cz echRepubic 2017/1, ASFV Moldova2017/1, ASFV Belgium 2018/1, ASFV Germany 2020/1, ASFV Arm/07/CBM/C2, ASFV Ken.riei 1, ASFV Wuhan 2019-2, ASFV _ HU _2018, ASF V/pig/China/CAS 19-01/2019; the whole gene sequence of the ASFV CN/GS/2018 isolate is shown in SE Q ID NO. 25.

Preferably, the amino acid sequence of the ASFV MGF-110-9L gene coding protein is shown in SEQ ID NO.2, the amino acid sequence of the ASFV MGF-505-7R gene coding protein is shown in SEQ ID NO.4, the amino acid sequence of the ASFV G-AC D-00190 gene coding protein is shown in SEQ ID NO.6, and the amino acid sequence of the ASFV G-ACD-00210 gene coding protein is shown in SEQ ID NO. 8.

Preferably, the nucleotide sequence of the ASFV MGF-110-9L gene is shown in SEQ ID NO.1, the nucleotide sequence of the ASFV MGF-505-7R gene is shown in SEQ ID NO.3, the nucleotide sequence of the ASFV G-ACD-00190 gene is shown in SEQ ID NO.5, and the nucleotide sequence of the ASFV G-ACD-00210 gene is shown in SEQ ID N O.7.

Preferably, the method for losing the functions of the proteins coded by the ASFV G-ACD-00190 gene, the ASFV MGF-110-9L gene, the ASFV G-ACD-00210 gene and the ASFV MGF-505-7R gene comprises a gene deletion technology, a gene mutation technology and a gene insertion technology.

In a second aspect, the invention provides an application of preparing an attenuated African swine fever virus strain or an African swine fever vaccine by jointly deleting a gene fragment in the genotype II African swine fever virus, wherein the gene fragment comprises all or part of a nucleotide sequence of an ASFV G-ACD-00190 gene, all or part of a nucleotide sequence of an ASFV MGF-110-9L gene, all or part of a nucleotide sequence of an ASFV G-ACD-00210 gene and all or part of a nucleotide sequence of an ASFV MGF-505-7R gene.

Preferably, the deleted gene segments are the 11627-12499 th site and 40752-42335 th site of the full-length sequence of the ASFV CN/GS/2018 isolate.

In a third aspect, the invention provides a gene deletion attenuated African swine fever virus strain, which is a gene type II African swine virus with a gene fragment deletion, wherein the gene fragment comprises all or part of a nucleotide sequence of an ASFV G-ACD-00190 gene, all or part of a nucleotide sequence of an ASFV MGF-110-9L gene, all or part of a nucleotide sequence of an ASFV G-ACD-00210 gene and all or part of a nucleotide sequence of an ASFV MGF-505-7R gene.

In a fourth aspect, the present invention provides an attenuated african swine fever vaccine comprising the attenuated african swine fever virus strain having the gene deletion according to the third aspect.

In a fifth aspect, the present invention provides a method for attenuating an African swine fever virus strain with a deleted gene according to the third aspect, wherein the method comprises deleting all or part of the nucleotide sequence of the ASFV G-ACD-00190 gene, all or part of the nucleotide sequence of the ASFV MGF-110-9L gene, all or part of the nucleotide sequence of the ASFV G-AC D-00210 gene and all or part of the nucleotide sequence of the ASFV MGF-505-7R gene in the original gene type II African swine fever virus by genetic engineering means.

Preferably, the method comprises the steps of:

(1) designing 1.5kb of upstream and downstream sequences of ASFV MGF-505-7R gene as left and right homologous recombination arms, cloning the left and right homologous recombination arm genes and eGFP gene screening expression cassette gene fragment p72-eGFP-SV40polyA to a pU C57 vector at the same time, and obtaining a recombinant plasmid p 53-eGFP;

(2) designing 1.5kb of upstream and downstream sequences of ASFV MGF-110-9L gene respectively as left and right homologous recombination arms, and cloning the left and right homologous recombination arms and mCherry gene screening expression cassette gene fragment p72-mCherry-NOSpolyA to a pU C57 vector at the same time to obtain recombinant plasmid p 20-mCherry;

(3) transfecting recombinant plasmids p53-eGFP and p20-mCherry to BMDM cells infected with ASFV original strains, and purifying and screening by adopting a plaque cloning method to obtain an attenuated African swine fever virus delta 20/53 with ASFV G-ACD-00190 gene, ASFV MGF-110-9L gene, ASFV G-ACD-00210 gene and ASFV MGF-505-7R gene combined deletion.

In a sixth aspect, the present invention provides an attenuated african swine fever virus produced by the method of the fifth aspect.

The invention has the beneficial effects that: the attenuated African swine fever virus strain is obtained by jointly losing the functions of ASFV G-ACD-00190 gene, ASFV MGF-110-9L gene, ASFV G-ACD-00210 gene and ASFV MGF-505-7R gene encoding protein, and has obvious attenuated virulence and good safety performance. By losing the functions of the ASFV G-ACD-00190 gene, the ASFV MGF-110-9L gene, the ASFV G-AC D-00210 gene and the ASFV MGF-505-7R gene encoding protein in parent strains, the toxicity of the parent strains is further reduced, the obtained attenuated African swine fever virus can provide complete immune protection effect on the attack of the ASFV parent strains, has high safety and is suitable for serving as a vaccine candidate strain for preventing African swine fever.

Drawings

FIG. 1 is a schematic diagram showing the construction of an ASFV MGF-505-7R gene deletion vector (express green fluorescence);

FIG. 2 is a schematic diagram showing the construction of ASFV MGF-110-9L gene deletion vector (express red fluorescence);

FIG. 3 shows that the African swine fever virus with gene deletion expresses red and green double-color fluorescent protein;

FIG. 4 shows the result of the purification and identification of African swine fever virus with gene deletion, wherein Δ 20/53 is African swine fever virus with deletion of gene; wherein 20 represents ASFV G-ACD-00190 gene, ASFV MGF-110-9L gene and ASFV G-ACD-00210 gene; 53 represents ASFV MGF-505-7R gene;

FIG. 5 shows the result of protein expression detection of African swine fever virus with gene deletion, wherein Δ 20/53 is African swine fever virus with gene deletion; wherein 20 represents ASFV G-ACD-00190 gene, ASFV MGF-110-9L gene and ASFV G-ACD-00210 gene; 53 represents ASFV MGF-505-7R gene;

FIG. 6 is a graph showing the body temperature changes of animals after the immunization of African swine fever virus strain delta 20/53 with gene deletion and after the challenge of ASFV CN/GS/2018 isolate, wherein the left side is the immunization part, the right side is the challenge part, each curve represents one animal, the abscissa is the immunization time and the challenge time, respectively, and the ordinate is the body temperature;

FIG. 7 is a graph showing the results of the virus-carrying in the blood of the animals immunized with the African swine fever virus strain Δ 20/53 and the live animals and the animals challenged after immunization, wherein the left side is the immunization part and the right side is the challenged part, each curve represents one animal, the abscissa represents the immunization time and the challenged time, and the ordinate represents the virus copy;

FIG. 8 shows the survival rate of experimental pigs immunized with the African swine fever virus strain Δ 20/53 or challenged with the ASFV CN/GS/2018 isolate, wherein the left side is the immunization part and the right side is the challenge part, each curve represents an animal, the abscissa represents the immunization time and challenge time, and the ordinate represents the survival rate;

FIG. 9 shows the result of IFN- β expression detection after infecting the immunized animals with the African swine fever virus strain Δ 20/53 and the parental strain with gene deletion.

Detailed Description

In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments. The scope of the invention is not limited to the examples described below.

The experiments described in the following examples obtain biosafety permits and african swine fever laboratory activity permits:

according to the related requirements of biosafety of a Lanzhou veterinary research institute of the national academy of agricultural sciences, a biological safety 3-level laboratory (BSL-3) and related biological safety of African swine fever, the Lanzhou veterinary research institute biological safety committee, the Experimental animal ethics committee, the Chinese academy of agricultural sciences biological safety committee, the Lanzhou veterinary research institute experimental animal ethics committee and the biological safety committee of the Lanzhou veterinary research institute report step by step, and the permission of developing highly pathogenic ASFV pathogeny and animal research is obtained by the agricultural department, and is already filed by the agricultural rural department and meets the requirements of the national biological safety level.

Experimental cell, viral and plasmid sources described in the following examples:

primary Porcine Alveolar Macrophages (PAM) and primary bone marrow macrophages (BMDM) were taken from healthy SPF Bama minipigs aged 2-4 months, aseptically collected, lysed with red blood cell lysate (purchased from Biosharp), red blood cells were removed, centrifuged at low speed, the supernatant was discarded, and the cell pellet was resuspended in RPMI 1640 complete medium (purchased from Gibco) containing 10% FBS (purchased from PAN), placed at 37 ℃ and 5% CO₂Culturing in an incubator. BMDM cell culture was supplemented with additional 10ng/mL final concentration of recombinant porcine GM-CSF (purchased from R) in RPMI 1640 complete medium&D Systems Co.), at 37 ℃ with 5% CO₂Inducing in an incubator, washing once every 2-3 days, centrifuging the nonadherent cells, adding into a new cell dish again, changing the liquid for continuous induction, and freezing for 3-7 days or using. ASFV amplification Using PAM cellsAnd titrating the virus content, and using the BMDM cell for plasmid transfection and virus recombination experiments.

The ASFV CN/GS/2018 isolate is from the national African swine fever regional laboratory (Lanzhou), belongs to genotype II, and can be obtained by entrustment letter approved by veterinary Bureau of Ministry of agriculture; the virus titer is 5 × 10⁷TCID₅₀and/mL, which is the 4 th generation virus after PAM cell propagation, and is subpackaged and stored at-80 ℃ for later use.

The peGFP-N1 vector and the pUC57 vector are purchased from Riborley Biotechnology, Inc. of Lanzhou; endotoxin-free plasmid extraction kit, purchased from OMEGA.

The procedures in the experiments are those known in the art unless otherwise specified.

Definition of

The term "loss of function of a protein" refers to the loss of function of a protein encoded by a gene by knocking out, mutating or inserting a part of the gene in a gene segment encoding the protein, so that the protein encoded by the gene is subjected to frame shift mutation.

The term "gene deletion" refers to the phenomenon that a segment of a chromosome and a gene carried by the segment are lost together to cause mutation.

The term "gene mutation" refers to a gene mutation, which means that the base pair composition or arrangement sequence of the gene is changed in structure, i.e., a new gene suddenly appears at a site, which replaces the original gene, and the gene is called a mutant gene, and the new trait never existed in the expression of the offspring suddenly appears due to the gene mutation. The gene deletion method generally refers to gene knock-out, and is an exogenous DNA introduction technique in which a DNA fragment containing a certain known sequence is subjected to homologous recombination with a gene having the same or similar sequence in the genome of a receptor cell, integrated into the genome of the receptor cell, and expressed. Methods of gene knockout generally include: homologous recombination technology, random insertion mutation technology and RNA interference technology; wherein, the homologous recombination technique is also called gene targeting, which means that the recombination occurs between the homologous sequence on the exogenous DNA and the chromosome DNA of the receptor cell, and the homologous sequence is integrated to a predetermined position, thereby changing some genetic characteristics, and the recombination aims at knocking out a certain gene; random insertion mutation technology is that certain viruses, bacteria or other gene vectors capable of randomly inserting gene sequences are utilized to carry out random insertion mutation on a cell bank in a target cell genome, and then corresponding gene knockout cells are obtained by screening through corresponding markers; the RN A interference technology refers to a reverse genetics technology which leads to the expression silencing of a target gene by the target mRNA degradation specifically initiated by double-stranded RNA homologous with the target gene mRNA endogenous to an organism.

The term "vaccine" refers to a biological agent capable of providing a protective response in an animal, wherein the vaccine has been delivered and is not capable of causing serious disease.

In the invention, by taking the gene II African swine fever virus strain ASFV CN/GS/2018 isolate AS an example, the ASFV G-ACD-00190 gene, the ASFV MGF-110-9L gene, the ASFV G-ACD-00210 gene and the ASFV MGF-505-7R gene are knocked out by the homologous recombination technology, but the ASFV G-ACD-00190 gene, the ASFV MGF-110-9L gene, the ASFV G-ACD-00210 gene and the AS FV MGF-505-7R gene can also be knocked out by the random insertion mutation technology and the RNA interference technology. However, the present invention is not limited to the ASFV CN/GS/2018 isolate. Those skilled in the art know that: after deletion of the relevant genes in the different ASFV genomes, the observed phenotypic differences correlate with the genetic makeup; and the existing epidemic gene II type African swine fever virus (including China/2018/AnhuiXCGQ, Pig/CN/HLJ/2018, Georgia2007, ASFV-SY18, ASFV/POL/2015/Podlaskie, ASFV/POL17_05838_ C220, A SFV/POL16_29413-o23, ASFV/POL16_20538_ o9, ASFV/POL17_04461_ C210, ASFV/POL16_20186_ o7, ASFV/POL17_03029_ C201, ASFV/POL16_20540_ o10, ASFV Czechubblic 2017/1, A SFV Moldova2017/1, ASFV Belgium 2018/1, ASFV 2020/1, ASFV/ARFV/M/70/CAS 2018, ASFV/CAS 2018/CAS 468, and ASFV/CAS 2018-WO 468. Therefore, on the basis of the same genetic composition of the African swine fever virus type II, the person skilled in the art can reasonably conclude that other African swine fever viruses of type II (including but not limited to China/2018/AnhuiXCGQ, Pig/CN/HLJ/2018, Georgia2007, ASFV-SY18, ASFV/POL/2015/Podlaskie, ASFV/POL17_ 05838C 220, ASFV/POL16_29413-o23, ASFV/POL16_20538_ o9, ASFV/POL17_04461_ C210, ASFV/POL16_20186_ o7, ASFV/POL 48 _03029_ C201, ASFV/Pol16_ zeo 10, ASFV/Cfv 2052 _ 2017/1, ASFV/CBfv _ 2017/1, ASFV/CBlvy 638/ASFV 638, and ASFV-Cl 638, ASFV Ken.rie1, ASFV Wuhan 2019-2, A SFV _ HU _2018, ASFV/pig/China/CAS19-01/2019) as starting strains can also successfully construct and obtain related attenuated African swine fever virus strains and vaccines.

Example 1 construction and purification identification of recombinant Virus Δ 20/53

1. Screening expression cassette construction

For ease of screening, expression cassettes for the selectable marker genes were constructed.

Construction of enhanced Green fluorescent protein (eGFP) gene screening expression cassette: reference is made to the literature (O' Donnell V, Holinka LG, Krug PW, Gladue DP, Carlson J, Sanford B, Alfano M, Kramer E, Lu Z, Arzt J, Reese B, Carrilo C, Risatti GR, BorcaMV. African Swine Feve Virus Georgia2007 with a Deletion of video-Associated Gene 9GL (B119L), where A dministered Low gases, lead to video amplification in Swine and industries impact P detection acquisition in Home Change JV. 2015; 89(16):8556-66) for PCR amplification of P72 promoter (sequence from P72 upstream to the alternative P72 +17 + SEQ ID NO); the amplification primers are as follows: a forward primer 5'-TTATAA AACATATGTTCATAAAAAGGGTCGCCGGAGGAAAAGTC-3' (shown in SEQ ID NO. 9) and a reverse primer 5'-CTCCTCGCCCTTGCTCACCATATATAATGTTATAAAAATAATT-3' (shown in SEQ ID NO. 10); and (3) amplifying the eGFP gene by using a peGFP-N1 vector as a template for later use, wherein amplification primers are as follows: a forward primer 5'-ATGG TGAGCAAGGGCGAGGAG-3' (shown in SEQ ID NO. 11) and a reverse primer 5'-ACCACAACTAGAATG CAGTG-3' (shown in SEQ ID NO. 12); according to the literature (Borca MV, Holinka LG, Berggren KA, Gladu DP. CRISPR-Cas9, a tool to effect amplification of the expression vector of recombinant African swine viruses rep.2018; 8(1):3154.), the two genes of the p72 promoter and the eGFP obtained by the amplification of the steps are connected by a fusion PCR method to obtain an eGFP screening expression cassette gene fragment which is named as p72-eGFP-SV40polyA and contains an SV 40A termination sequence.

Construction of a screening expression cassette of a red fluorescent protein gene mCherry: a pcDNA 3-mChery vector is used as a template to amplify a red fluorescent protein mChery gene, a forward primer 5'-ATGGTGAGCAAGGGCGAGGAG-3' (shown in SEQ ID NO. 13) and a reverse primer 5'-TTACTTGTACAGCTCGTCCATGC-3' (shown in SEQ ID NO. 14), and meanwhile, a synthetic Nopaline synthase (NOS) termination sequence (Genbank accession number is AB697057.1), namely 1870-2192nt is used as a transcription termination sequence of a p72 promoter and is named as an NOSpolyA sequence, a p72 promoter, the mChery gene sequence and the NOSpolyA sequence are connected through fusion PCR to obtain an mChery screening expression cassette gene segment which is named as p 72-mChery-NOSpolyA.

2. Construction of homologous recombination transfer vector

A pUC57 vector is used as a skeleton vector to construct an ASFV G-ACD-00190 gene, an ASFV MGF-110-9L gene, an ASFV G-ACD-00210 gene and an ASFV MGF-505-7R gene knockout homologous recombination transfer vector, wherein the nucleotide sequence of the ASFV MGF-110-9L gene is shown in SEQ ID NO.1, the nucleotide sequence of the ASFV MGF-505-7R gene is shown in SEQ ID NO.3, the nucleotide sequence of the ASFV G-ACD-00190 gene is shown in SEQ ID NO.5, and the nucleotide sequence of the ASFV G-ACD-00210 gene is shown in SEQ ID NO. 7.

The deleted gene sequences are respectively positioned at the 11627-12499 site and 40752-42335 site of the ASFV CN/GS/2018 isolate whole gene sequence, and the construction strategies are shown in figure 1 and figure 2.

The method comprises the following specific steps: because the ASFV G-ACD-00190 gene and the ASFV G-ACD-00210 gene are positioned at two ends of the ASFV M GF-110-9L gene and are partially overlapped with the ASFV MGF-110-9L gene (a partial sequence and a promoter sequence of CDS), an upstream sequence 1.5kb of an ASFV MGF-110-9L gene fragment is designed to be used as a homologous recombination Left arm (Left ar M, shown by SEQ ID NO. 15) and a downstream sequence 1.5kb is designed to be used as a homologous recombination Right arm (Right arm, shown by SEQ ID NO. 16), and the upstream sequence and the downstream sequences are respectively cloned into a pUC57 vector to obtain a recombination transfer vector of the ASFV MGF-110-9L genome fragment; inserting mCherry screening expression cassette gene fragment p72-mCherry-NOSpolyA in the middle of the gene sequences of the left arm and the right arm of a recombinant transfer vector of an ASFV MGF-110-9L genome fragment; after the sequencing is correct, the homologous recombination transfer vector is named as p 20-mCherry; extracting DNA with endotoxin-free plasmid extraction kit, measuring concentration, and storing at-20 deg.C;

designing upstream and downstream sequences of ASFV MGF-505-7R gene each with 1.5kb as homologous recombination Left arm (Left arm shown in SEQ ID NO. 17) and Right arm (Right arm shown in SEQ ID NO. 18), and cloning into pUC57 vector respectively to obtain recombination transfer vector of ASFV MGF-505-7R gene; inserting eGFP screening expression box gene fragment p72-eGFP-SV40polyA in the middle of the gene sequences of the left arm and the right arm of a recombinant transfer vector of ASFV MGF-505-7R gene; after the sequencing is correct, the homologous recombination transfer vector is named as p 53-eGFP; extracting DNA with endotoxin-free plasmid extraction kit, measuring concentration, and storing at-20 deg.C.

3. Cell transfection and recombinant virus screening

Homologous recombination transfer vectors P53-eGFP and

and (3) sufficiently and uniformly mixing the Macrophage DNA transfection reagent, co-transfecting to well-conditioned bone marrow Macrophage (BMDM) cells of healthy SPF Bama miniature pigs of 2-4 months old, and directly infecting the 1 MOI ASFV CN/GS/2018 purified virus strain to obtain the ASFV recombinant virus with MGF-505-7R gene deletion. Then p20-mCherry is transfected into BMDM cells, ASFV recombinant virus with MGF-505-7R gene deletion is infected, and the virus strain is purified to obtain the gene deletion virus.

The observation result under the microscope is shown in fig. 3, and the result shows that the transfected and purified gene-deleted virus expresses red-green double-color fluorescence when infecting BMD M cells, which indicates that the recombinant virus is successfully constructed.

And (3) detecting the purity: selecting an MGF-110-9L-check-F/R primer pair (MGF-110-9L-check-F: ACATACACC GGTCATGCCAC (shown in SEQ ID NO. 19) and MGF-110-9L-check-R: TACCAATGACCCGGAG ACCT (shown in SEQ ID NO. 20)); the 53-check-F/R primer pair (53-check-F: TTTGGGAAAATCCC GCGGAAAGAA (shown in SEQ ID NO. 21) and 53-check-R: TCCTGTAGGGAGAACATTTTCTC T (shown in SEQ ID NO. 22)) was subjected to purity detection. Meanwhile, p72 in the virus genome is selected as an internal reference gene to ensure the quality of the detected virus genome, and primers for p72 detection are p 72-F: CGGGGGTTTTAATCGCATT GC (shown in SEQ ID NO. 23) and p 72-R: CGGGGGTTTTAATCGCATTGC (shown in SEQ ID NO. 24).

The results are shown in figure 4, and the primers in endogenous ASFV MGF-110-9L gene and ASFV MGF-505-7R gene are used for detecting whether the gene deletion attenuated African swine fever virus is successfully constructed, wherein wt is ASFV CN/GS/2018 isolate, and delta 20/53 represents the gene deletion attenuated African swine fever virus. The result shows that ASFV MGF-110-9L gene and ASFV MGF-505-7R gene can not be detected in the genome of the African swine fever virus with gene deletion and attenuation, which indicates that the ASFV MGF-110-9L gene and the ASFV MGF-505-7R gene are successfully knocked out; the above results indicate that the gene deletion attenuated African swine fever virus has been successfully constructed and has been purified and named delta 20/53.

The results of protein expression detection after infection of cells with the gene-deleted virus strain Δ 20/53 and the parental strain are shown in FIG. 5, in which 53 represents the protein encoded by the ASFV MGF-505-7R gene, and the results indicate that the expression of the protein encoded by the ASFV MGF-110-9L gene and the ASFV MGF-505-7R gene is not detected after infection of cells with the gene-deleted virus strain Δ 20/53, that is, the protein encoded by the AS FV MGF-110-9L gene and the ASFV MGF-505-7R gene is functionally lost. Because the promoter sequences of the AS FV G-ACD-00190 gene and the ASFV G-ACD-00210 gene are deleted, the proteins coded by the ASFV G-ACD-00190 gene and the ASFV G-ACD-00210 gene cannot be normally expressed, and the function of the proteins coded by the ASFV G-ACD-00190 gene and the ASFV G-ACD-00210 gene is lost.

Example 2 determination of viral titre

The titer of African swine fever virus adopts half of blood cell adsorption amount (50% haemadsorption, HAD)₅₀) Denotes, HAD₅₀The concrete operation of the experiment is shown in the literature(Borca MV, Ramirez-Medina E, Silva E, Vuono E, Rai A, Pruitt S, Holinka LG, Velazquez-Salinas L, Zhu J, Gladeu DP. development of a high effective active area wind turbine vitamin b. duration of the I177L gene responses in stereo immunity against the current therapeutic European strain. JVirol.2020.pii: JVit I.02017-19) with appropriate adjustments: at about 1X10 in 96-well plates⁵Inoculating primary PAM cells to the cells in each hole, continuously diluting the recombinant virus to be detected by 10-fold gradient for 7 dilutions, adding the diluted virus to PAM in a 96-hole plate according to 100 mu L/hole, immediately adding red blood cells, and repeating for three times. The virus infection can be determined according to rosette formed by erythrocyte aggregation around infected cell, continuously observing for 6 days, counting number of positive holes, and calculating half number of blood cell adsorption amount (HAD)₅₀). And (4) determining the titer to be qualified, and evaluating the pathogenicity.

Example 3 evaluation of virulence of Gene deleted Strain Δ 20/53

In order to detect the toxicity of the gene deletion strain delta 20/53, 10 is used in the experiment⁴HAD₅₀The dose was evaluated for its virulence by intramuscular injection to piglets.

The 12 African swine fever antigen antibody negative healthy long and white piglets used in the experiment are divided into 2 groups in total, wherein 6 groups of gene deletion strain delta 20/53 immune groups and 6 groups of co-living groups are adopted. Body temperature changes were measured daily following immunization, peripheral blood and saliva collected, reference (King DP, Reid SM, Hutchings GH, Grierson SS, Wilkinson PJ, Dixon LK, bases A D, drive TW.2003.development of a TaqMan PCR assay with internal amplification control alcohol for the detection of aircraft shock virus. J Virus Methods 107:53-61), and ASFV virus content in blood was determined by fluorescent quantitative PCR method and was terminated by 17 days. The lethality of the animals in the immune group and the animals in the same living group is counted, the result of the change of the body temperature is shown in figure 6 (immune part) and the survival rate is shown in figure 8 (immune part), after the gene deletion strain delta 20/53 is injected into the muscle, the body temperature of the pigs in the immune group and the pigs in the same living group are normal, continuous high temperature does not occur, death does not occur, and the survival rate is 100%.

The results of detection of the content of ASFV virus in blood are shown in fig. 7 (immune part), and after injection of gene deletion strain Δ 20/53, the immunized group of pigs detected a small amount of virus in blood 7 days after immunization, but no blood virus was detected by 17 days after immunization; in addition, no virus-carrying condition was detected in blood of the pigs in the same dwelling group, and virus detection results in organs of the pigs in the same dwelling group were negative 17 days after immunization (the results are shown in table 1). The experimental results show that after the ASFV G-ACD-00190 gene, the ASFV MGF-110-9L gene ASFV G-ACD-00210 gene and the ASFV MG F-505-7R gene coding protein function is lost in a parent ASFV CN/GS/2018 isolate, the obtained gene deletion strain delta 20/53 has completely weakened virulence, no virus elimination and better safety.

TABLE 1 toxicity of the tissues of the same dwelling animals

Note: n indicates that the detection result is negative

Example 4 measurement of expression of serum IFN-. beta.after immunization of animals with deleted Virus. DELTA. 20/53

The inventor firstly finds that proteins coded by ASFV MGF-110-9L gene and ASFV MGF-505-7R gene have immune suppression effect and can reduce the expression level of IFN-beta. Therefore, this example examined whether or not the immunosuppressive functions of the proteins encoded by the ASFV MGF-110-9L gene and the ASFV MGF-505-7R gene were lost by the following experiment.

Are respectively used 10⁴HAD₅₀After the immune animal is infected by the gene deletion virus strain delta 20/53 and the parent strain in the dosage, the expression level of IFN-beta in serum of the immune animal is detected by using an ELISA kit, and the result is shown in figure 9, wherein Mock is a healthy pig, and the result shows that the expression level of IFN-beta in serum is remarkably reduced after the immune pig is infected by the parent strain compared with the healthy pig, while the serum of the immune pig is infected by the gene deletion virus strain delta 20/53 compared with the parent strainThe expression level of IFN- β in (a) is significantly increased. The above results indicate that the function of the proteins encoded by ASFV MGF-110-9L gene and ASFV MGF-505-7R gene in gene-deleted virus strain Δ 20/53 is lost, and the immunosuppressive effects of ASFV MGF-110-9L gene and ASFV MGF-505-7R gene are released.

Example 5 evaluation of immunoprotective Effect of deleted Virus.DELTA. 20/53

In order to test the immunoprotection effect of the gene deletion strain delta 20/53, 10 is used in the experiment²HAD₅₀Dose of parent ASFV CN/GS/2018 isolate A challenge experiment was performed on delta 20/53 immunized pigs (6 pigs) and non-immunized control pigs (6 pigs) from example 3.

After challenge, the temperature change was measured daily, peripheral blood was collected and observed to end in 13 days. The survival rate results are shown in fig. 8 (toxin attacking part), wherein the abscissa is the toxin attacking time, the ordinate is the survival rate, after the parent ASFV CN/GS/2018 isolate is attacked, the survival rate of the immunized pigs is 100%, and the control pigs all die from the toxin attacking to the 5 th day. The results of body temperature changes are shown in fig. 6 (challenge section), and the immune group pigs did not show typical symptoms of fever, while the control group pigs had a sharp rise in body temperature after challenge and all died by day 5. The results show that after the parent ASFV CN/GS/2018 isolate is detoxified, the body temperature of the pig in the gene deletion strain delta 20/53 immune group is normal, the survival rate is 100 percent, namely the gene deletion strain delta 20/53 has complete immune protection effect on the parent ASFV CN/GS/2018 isolate.

The present experiment was carried out by measuring the ASFV virus content in blood by a fluorescent quantitative PCR method and detecting the blood-borne toxicity of post-immune and non-immune challenge pigs, according to the literature (King DP, Reid SM, Hutchings GH, Grierson SS, Wilkinson PJ, Dixo n LK, bases AD, drive TW.2003.development of a TaqMan PCR assay with internal a amplification control for the detection of the infection of the animal swine virus 107: 53-61). The blood toxicity of the immunized group and the non-immunized group is shown in fig. 7 (toxicity attacking part), wherein the abscissa is the toxicity attacking time, the ordinate is the ASFV virus content, and the control group pigs all died from the toxicity attacking to day 5. Experimental results show that only 1 pig blood in an immune group has a small amount of virus within 1-5 days after the ASFV CN/GS/2018 isolate is detoxified, and the virus in the blood of animals in the immune group is basically not detected after 5 days.

In order to detect the toxic condition in each tissue of the immunized pigs, each tissue sample of the experimental animals was taken 13 days after the immunized pigs were attacked. The virus content in the tissues is detected, except that the heart tissue of the No.1 pig and the lung tissue of the No.2 pig are suspicious, the detection results are shown in the table 2, and the virus separation in each organ of the immunized pig is negative; since all the control pigs died from the challenge to day 5, the visceral organs of the control pigs were not counted. The results further indicate that the gene deletion strain ASFV delta 20/53 has complete immune protection effect on the parent ASFV CN/GS/2018 isolate.

TABLE 2 tissue toxicity after challenge of ASFV delta 20/53 strain immunized animals

Animal numbering

Heart with heart-shaped

Liver disease

Spleen

Lung (lung)

Kidney (Kidney)

Submaxillary lymph

Mesenteric lymph

1

Suspicious (34.0)

N

2

N

Suspicious (34.2)

N

3

N

4

N

5

N

6

N

Note: n indicates that the detection result is negative (CT is more than 35.0) and suspicious (CT is more than 34.0 and less than 35.0)

In conclusion, on the basis of successfully constructing the attenuated African swine fever virus with the ASFV G-ACD-00190 gene, the ASFV MGF-110-9L gene, the ASFV G-ACD-00210 gene and the ASFV MGF-505-7R gene encoding protein function loss by the homologous recombination technology, researchers can simultaneously make the ASFV G-ACD-00190 gene, the ASFV MGF-110-9L gene, the ASFV G-ACD-00210 gene, the ASFVF-505-7R gene and one or more of the disclosed virulence genes (CD2V gene, MGF-360-12L gene, MGF-360-13L gene, UK gene, MGF-360-14L gene or MGF-360-505-R gene and the like) encoding protein function loss, finally, the safer and more effective African swine fever vaccine is prepared.

Sequence listing

<110> Lanzhou veterinary research institute of Chinese academy of agricultural sciences

Construction of <120> African swine fever polygene combined deletion attenuated strain and application of attenuated strain as vaccine

<150> CN202010662107.8

<151> 2020-07-10

<160> 25

<170> SIPOSequenceListing 1.0

<210> 1

<211> 873

<212> DNA

<213> African swine fever virus (African swine fever virus)

<400> 1

atgaaggtga ttgtgttcct tttggtactg gcggtcatgc agccggtcat tcaaagccaa 60

tcttttccag gaacaggaga gttaccaatg acccggagac ctccaaagag agagcttgag 120

tattggtgca cctatgcaaa atcgtgtgac ttctgctgga attgtcgaca cggggtttgt 180

aaaaataagg tttttgaaaa acaccctctc atcaaaaaaa atgattacat acaaatatgt 240

agggtttctc gctataatga aagatgtagc tactttacag actctaggat acgccgcttt 300

cacatcatga gctgtacaaa tcccacatat tatgattggt ttgatgagtt aatgcaaata 360

aaggaggata gggtcattga cactgagaat atcaaacata cttgtctttg tatgatagct 420

accattgctc tcataagcta tgttcgcaaa caatactcac gaatgcgaat gcaagccgct 480

acaagactgc ttatctttct tggcttctat gttcttttag gaattttgat gacgaacata 540

ataatgaacc tacctctttc cacagataat ccgatgcaaa tgcgtaggcc tcctgaaagg 600

gatctcaagt tctggtgcac ctatgcaaaa cactgtgact tctgctggac ctgtaaagat 660

ggaatgtgta aaaataaagt gtttagtgac caccctatta ttacgcaaaa tgattatatt 720

gttaattgta cagtttcccg gtggcatgac cggtgtatgt atgaagctca ctttaggata 780

cactatcaac ataacatgaa ttgttcacaa cccaaagatt tagaatggtt cattgagtta 840

aaacgacatg tgattaatca agatgatttg taa 873

<210> 2

<211> 290

<212> PRT

<213> African swine fever virus (African swine fever virus)

<400> 2

Met Lys Val Ile Val Phe Leu Leu Val Leu Ala Val Met Gln Pro Val

1 5 10 15

Ile Gln Ser Gln Ser Phe Pro Gly Thr Gly Glu Leu Pro Met Thr Arg

20 25 30

Arg Pro Pro Lys Arg Glu Leu Glu Tyr Trp Cys Thr Tyr Ala Lys Ser

35 40 45

Cys Asp Phe Cys Trp Asn Cys Arg His Gly Val Cys Lys Asn Lys Val

50 55 60

Phe Glu Lys His Pro Leu Ile Lys Lys Asn Asp Tyr Ile Gln Ile Cys

65 70 75 80

Arg Val Ser Arg Tyr Asn Glu Arg Cys Ser Tyr Phe Thr Asp Ser Arg

85 90 95

Ile Arg Arg Phe His Ile Met Ser Cys Thr Asn Pro Thr Tyr Tyr Asp

100 105 110

Trp Phe Asp Glu Leu Met Gln Ile Lys Glu Asp Arg Val Ile Asp Thr

115 120 125

Glu Asn Ile Lys His Thr Cys Leu Cys Met Ile Ala Thr Ile Ala Leu

130 135 140

Ile Ser Tyr Val Arg Lys Gln Tyr Ser Arg Met Arg Met Gln Ala Ala

145 150 155 160

Thr Arg Leu Leu Ile Phe Leu Gly Phe Tyr Val Leu Leu Gly Ile Leu

165 170 175

Met Thr Asn Ile Ile Met Asn Leu Pro Leu Ser Thr Asp Asn Pro Met

180 185 190

Gln Met Arg Arg Pro Pro Glu Arg Asp Leu Lys Phe Trp Cys Thr Tyr

195 200 205

Ala Lys His Cys Asp Phe Cys Trp Thr Cys Lys Asp Gly Met Cys Lys

210 215 220

Asn Lys Val Phe Ser Asp His Pro Ile Ile Thr Gln Asn Asp Tyr Ile

225 230 235 240

Val Asn Cys Thr Val Ser Arg Trp His Asp Arg Cys Met Tyr Glu Ala

245 250 255

His Phe Arg Ile His Tyr Gln His Asn Met Asn Cys Ser Gln Pro Lys

260 265 270

Asp Leu Glu Trp Phe Ile Glu Leu Lys Arg His Val Ile Asn Gln Asp

275 280 285

Asp Leu

290

<210> 3

<211> 1584

<212> DNA

<213> African swine fever virus (African swine fever virus)

<400> 3

atgttctccc ttcaggacct ctgtcggaag aacaccttct tccttccaag tgattttagc 60

aagcataccc tgcatttgct ggggttatac tggaaggggc atggatctat ccaaaggata 120

aagaatgatg gtgtgcttat agagcatgat cttactcttt ccatcaatga agccttaatt 180

cttgcaggag aagagggaaa caatgaagta gtaaagctct tgttactatg ggaaggaaat 240

cttcattatg ccatcatagg agctttgagg actgagaact ataacctagt atgtgagtac 300

catagtcaaa ttcaggactg gcatgttctc ctccctttga ttcaagatcc agaaacattc 360

gaaaaatgtc atgatttaag ccttgaatgt gatctttcat gccttctcca acatgctgta 420

aaatataaca tgctttcgat tcttgttaaa tataaagagg atctactaaa tgtactattt 480

aggcaacaaa ttcaaggact atttatttta gcatgtgaaa atcggaagct tgagattctt 540

acgtggatgg gtcaaaatct gccaattcct gatcctgagc ctatttttag cattgctgtt 600

gtcacaaaag atttagaaat gttttcctta gggtacaaga ttgtttttga atacatggaa 660

aaccaaggac ttcatttaac ccaggtagtt cgtatggtta tgctaaatca tcactttggc 720

atggtaataa ataaaggact tttacccttt gtgctggaaa ttttaaatta tggtgggaat 780

gtaaatagag ccttatctta tgctgtcaca caaaataaaa gaaagatttt agaccatgtt 840

gttcgccaaa agaatatacc ccataaaacc attgaaagaa tgttgcatct ggctgtaaaa 900

aagcatgctc ccaggaaaac tctgaacttg ttactatctt acataaatta caaggtgaaa 960

aatgttaaaa agttgttaga acatgtagtg aaatacaact ctactcttgt gataagactc 1020

ttgttagaaa aaaagaaaaa cctgctggat gctactttga caagatatgt caaagattct 1080

acatactttc aggtgaaaga atttatgcaa gacttctcca tcagcccaga aaaattcatt 1140

aaaatagctg tgcgggaaaa gagaaatgtg ttgatcaagg gtatttctga agatatttgg 1200

gaaaatcccg cggaaagaat caggaatctt aagcagatag tgtgtaccat aaaatatgaa 1260

agtggaagac aattcctgat aaatatcatt cacaccattt accagagtta ttctttgaaa 1320

cctgaagaaa ttcttaaatt ggcaacattt tatgtcaaac acaatgcaac cacccatttt 1380

aaagatctct gcaaatatct ttggctgaac agaagaacag aaagtaagaa actgttttta 1440

gagtgcttgg aaattgctga taagaaggag tttcctgata ttaaaagtat tgtgagtgaa 1500

tacattaact atttgtttac tgcaggagct attaccaagg aagaaatcat gcaagcctat 1560

gctttggagt atgccatgta ttaa 1584

<210> 4

<211> 527

<212> PRT

<213> African swine fever virus (African swine fever virus)

<400> 4

Met Phe Ser Leu Gln Asp Leu Cys Arg Lys Asn Thr Phe Phe Leu Pro

1 5 10 15

Ser Asp Phe Ser Lys His Thr Leu His Leu Leu Gly Leu Tyr Trp Lys

20 25 30

Gly His Gly Ser Ile Gln Arg Ile Lys Asn Asp Gly Val Leu Ile Glu

35 40 45

His Asp Leu Thr Leu Ser Ile Asn Glu Ala Leu Ile Leu Ala Gly Glu

50 55 60

Glu Gly Asn Asn Glu Val Val Lys Leu Leu Leu Leu Trp Glu Gly Asn

65 70 75 80

Leu His Tyr Ala Ile Ile Gly Ala Leu Arg Thr Glu Asn Tyr Asn Leu

85 90 95

Val Cys Glu Tyr His Ser Gln Ile Gln Asp Trp His Val Leu Leu Pro

100 105 110

Leu Ile Gln Asp Pro Glu Thr Phe Glu Lys Cys His Asp Leu Ser Leu

115 120 125

Glu Cys Asp Leu Ser Cys Leu Leu Gln His Ala Val Lys Tyr Asn Met

130 135 140

Leu Ser Ile Leu Val Lys Tyr Lys Glu Asp Leu Leu Asn Val Leu Phe

145 150 155 160

Arg Gln Gln Ile Gln Gly Leu Phe Ile Leu Ala Cys Glu Asn Arg Lys

165 170 175

Leu Glu Ile Leu Thr Trp Met Gly Gln Asn Leu Pro Ile Pro Asp Pro

180 185 190

Glu Pro Ile Phe Ser Ile Ala Val Val Thr Lys Asp Leu Glu Met Phe

195 200 205

Ser Leu Gly Tyr Lys Ile Val Phe Glu Tyr Met Glu Asn Gln Gly Leu

210 215 220

His Leu Thr Gln Val Val Arg Met Val Met Leu Asn His His Phe Gly

225 230 235 240

Met Val Ile Asn Lys Gly Leu Leu Pro Phe Val Leu Glu Ile Leu Asn

245 250 255

Tyr Gly Gly Asn Val Asn Arg Ala Leu Ser Tyr Ala Val Thr Gln Asn

260 265 270

Lys Arg Lys Ile Leu Asp His Val Val Arg Gln Lys Asn Ile Pro His

275 280 285

Lys Thr Ile Glu Arg Met Leu His Leu Ala Val Lys Lys His Ala Pro

290 295 300

Arg Lys Thr Leu Asn Leu Leu Leu Ser Tyr Ile Asn Tyr Lys Val Lys

305 310 315 320

Asn Val Lys Lys Leu Leu Glu His Val Val Lys Tyr Asn Ser Thr Leu

325 330 335

Val Ile Arg Leu Leu Leu Glu Lys Lys Lys Asn Leu Leu Asp Ala Thr

340 345 350

Leu Thr Arg Tyr Val Lys Asp Ser Thr Tyr Phe Gln Val Lys Glu Phe

355 360 365

Met Gln Asp Phe Ser Ile Ser Pro Glu Lys Phe Ile Lys Ile Ala Val

370 375 380

Arg Glu Lys Arg Asn Val Leu Ile Lys Gly Ile Ser Glu Asp Ile Trp

385 390 395 400

Glu Asn Pro Ala Glu Arg Ile Arg Asn Leu Lys Gln Ile Val Cys Thr

405 410 415

Ile Lys Tyr Glu Ser Gly Arg Gln Phe Leu Ile Asn Ile Ile His Thr

420 425 430

Ile Tyr Gln Ser Tyr Ser Leu Lys Pro Glu Glu Ile Leu Lys Leu Ala

435 440 445

Thr Phe Tyr Val Lys His Asn Ala Thr Thr His Phe Lys Asp Leu Cys

450 455 460

Lys Tyr Leu Trp Leu Asn Arg Arg Thr Glu Ser Lys Lys Leu Phe Leu

465 470 475 480

Glu Cys Leu Glu Ile Ala Asp Lys Lys Glu Phe Pro Asp Ile Lys Ser

485 490 495

Ile Val Ser Glu Tyr Ile Asn Tyr Leu Phe Thr Ala Gly Ala Ile Thr

500 505 510

Lys Glu Glu Ile Met Gln Ala Tyr Ala Leu Glu Tyr Ala Met Tyr

515 520 525

<210> 5

<211> 144

<212> DNA

<213> African swine fever virus (African swine fever virus)

<400> 5

atgatttgta actataagtt tgtttttttt tcttattttg caacgggtaa tattaaatgg 60

tggcctgttc atttatttaa caaaaaaata ttaacaaatt tgttattaag cctgtcgctg 120

acatatcctg taaccattct ataa 144

<210> 6

<211> 47

<212> PRT

<213> African swine fever virus (African swine fever virus)

<400> 6

Met Ile Cys Asn Tyr Lys Phe Val Phe Phe Ser Tyr Phe Ala Thr Gly

1 5 10 15

Asn Ile Lys Trp Trp Pro Val His Leu Phe Asn Lys Lys Ile Leu Thr

20 25 30

Asn Leu Leu Leu Ser Leu Ser Leu Thr Tyr Pro Val Thr Ile Leu

35 40 45

<210> 7

<211> 192

<212> DNA

<213> African swine fever virus (African swine fever virus)

<400> 7

atggctgcaa cttataagtt gcaacttatg ggttgcaata ctgcaacgta taggttgcac 60

cttatagatc gcgactcaaa aggtatgaaa accttaccct caatacagaa tttaagtttt 120

aatcctgata atgtatctgt ttatgaaaaa aaattttttt tactcatgta tgaattctta 180

tacgaatcat aa 192

<210> 8

<211> 63

<212> PRT

<213> African swine fever virus (African swine fever virus)

<400> 8

Met Ala Ala Thr Tyr Lys Leu Gln Leu Met Gly Cys Asn Thr Ala Thr

1 5 10 15

Tyr Arg Leu His Leu Ile Asp Arg Asp Ser Lys Gly Met Lys Thr Leu

20 25 30

Pro Ser Ile Gln Asn Leu Ser Phe Asn Pro Asp Asn Val Ser Val Tyr

35 40 45

Glu Lys Lys Phe Phe Leu Leu Met Tyr Glu Phe Leu Tyr Glu Ser

50 55 60

<210> 9

<211> 44

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 9

ttataaaaca tatgttcata aaaagggtcg ccggaggaaa agtc 44

<210> 10

<211> 43

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 10

ctcctcgccc ttgctcacca tatataatgt tataaaaata att 43

<210> 11

<211> 21

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 11

atggtgagca agggcgagga g 21

<210> 12

<211> 20

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 12

accacaacta gaatgcagtg 20

<210> 13

<211> 21

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 13

atggtgagca agggcgagga g 21

<210> 14

<211> 23

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 14

ttacttgtac agctcgtcca tgc 23

<210> 15

<211> 1500

<212> DNA

<213> African swine fever virus (African swine fever virus)

<400> 15

aacagcatga aatacaagag tgcctgttac acgaataagt tctctcaaac cggggatggt 60

catactcaca tctatgaaat cctggtctag gagattcatt tgatgcatga tggccgcacc 120

cacacttatg agacactgaa gaactaaagg gtttaatttt gatctgaatg gtactatata 180

ggatgatggc aatccatatc aagattagag caatcaaaat cacctcctca agaagcatga 240

tgtagcctta aatcttagac tgctttaaac cttaggccct cactatcttt aatgaaggag 300

tttaaatttt gatccctttt tcaagaccca tttagaagaa aaaaataaag tttatatcaa 360

tctaattcat aagtcatctc tttcataaat cttcatgtat tctctatgtg gataagtatg 420

ggatgttgga tttgcgcagt ccatttgatg atctgtatgg tttttgggtc cttcataata 480

actacatata ccattccagc gggaaaccgt gcaatttata atccagtcat tttgatgaat 540

aactggccaa tctgtttgaa tcctgtttcg gcagataccg tggacgcatt cccagcaaaa 600

gtcacattgg tttgcgtaag tgcaccaata aactagctca tgttcaggag gataacgggt 660

tggtagtaaa tcttctaatt tacgtatagg agcggcttga aggacaacca cccccagtag 720

tactagaatc agtaccttta tagtggccac cctacactag acctctaagt tgaagacaaa 780

gaactaaaat ttagagccgt ttaattacta ctaataatta tattttttat tgtctacaat 840

aggattctat taaaaaataa tgatttttac caagaaatat ttttataaaa aattaatata 900

ttttgtaata aactttattt ccaatgactg ttaaaataag gaaactatcc ttagttagtc 960

gaggaagatg gttaggttat ttcgcaatcc gataaaatgt ttattttatc gtaggtctcg 1020

taaaatccag gaaaaaaaat tacggaagag tttaaaaaag ctaaattttt accaccctcc 1080

agaagattgt tgtcaaatat atcgtttgct agaaaatgtt cctggaggaa cttactttat 1140

tacagaaaat atgacgaatg atttaattat ggtcgtaaag gattcggtgg ataaaaaaat 1200

taaaagcatt aaattatatc ttcatggaag ttatattaag attcatcagc actattatat 1260

taatatttat atgtatctta tgagatatac ccaaatttat aaatatccct taatttgttt 1320

taacaaatat tataacatct aagtaaatat tcttggaatg gattttctta tagaatggtt 1380

acaggatatg tcagcgacag gcttaataac aaatttgtta atattttttt gttaaataaa 1440

tgaacaggcc accatttaat attacccgtt gcaaaataag aaaaaaaaac aaacttatag 1500

<210> 16

<211> 1500

<212> DNA

<213> African swine fever virus (African swine fever virus)

<400> 16

ggctgcaact tataagttgc aacttatggg ttgcaatact gcaacgtata ggttgcacct 60

tatagatcgc gactcaaaag gtatgaaaac cttaccctca atacagaatt taagttttaa 120

tcctgataat gtatctgttt atgaaaaaaa atttttttta ctcatgtatg aattcttata 180

cgaatcataa tatgtaggct gagaataata attcatatac ggtgttgcgg gctcaataaa 240

aattttgtta ccacaaaaaa taaatgctgg atttttaaga tatatatcta ttaatgacta 300

aaccctttat acgctgtagg ctgaaaacaa tccatataat gaatatacgg tgatttgggt 360

ttaataaaat acatacaacg gtcaaaatag cgggcaatac tacattgact aatataatca 420

ttttgtttaa taagaggcat atcatcccac actttatttt tacaaatacc gttcctacat 480

tcccagcaga aatcacagtg ttttccatac gtgcaccagt attcaagctc tcttatagga 540

ggcgtataag tccttggtaa attttgtttc atataaaaga tggaaagggg tcgatttaaa 600

cccggctgag atagccaaat caaaatacat aaaagagcaa gtagtttcat agtggtattt 660

agatgtaaat ttttatagta tgcaaataca atgtaaccta caaatacaat actaaataca 720

aggtaaaaac aacaatgtct tataatgatt ggccaataat cacccccccc ccccccattt 780

ttccatgaat atttcatttc ctgtataggg tctaggatgt gaacactcca tgttatgatg 840

attaggcatt ttaactgata tttcataaaa acacccccag gaattgcgat taactataca 900

gtttacaatc gaattcatcg aattagactc atttgttatc ttatttttac aaatgccatt 960

ttgacaatcc cagcagaagt cacaattctt tacatacgta caccaatatg gaagctcctc 1020

cttaggagga tgctgggttc ttggtaattc tggtaattca tgtgcaagaa tgaggactga 1080

gtagcccaac aaaagtccta gaaccttcat gttgtgtcca aatggcacct gtcattttaa 1140

aaaagattta aattttgcta ccgcaaaaaa aaatccagta tgtatttttt taatacatat 1200

aattattgaa gtcttataag ataaagccga gaacactata ttttgtatag atgatgtatc 1260

cggtattcaa actctcttat aagtacatgt aggaaatggt caattattca agattggctg 1320

agataacaac aaaaccaaaa tactcaaaag cataagtaat ttcatggttg tactcagtcg 1380

tagatttttg cagatcgcaa atgcaacgca accagcaaat acaaagctaa atacaaggta 1440

aaaacaataa taccttataa tgattggcca attcttatcc ctccattttt ccatgaacat 1500

<210> 17

<211> 1500

<212> DNA

<213> African swine fever virus (African swine fever virus)

<400> 17

gtagtacagc tcttgttatt atgggaggga aatctgcatt atgccatcat aggagccttg 60

aagactgaaa aatataacct aatatgtgag tatcatagcc aaattcagga ctggcatatt 120

ctcctaccca tgattcaaga tccagaaaca ttcgaaaaat gtcatgattt aagccttgga 180

tgtgacttta tttgccttct ccaacatgct gtaaaataca acatgctttc tattcttgtc 240

aaatataagg aggatctact aaatgcaagg attaggcatc gtatccaatc cctgtttgtt 300

ttggcatgcg aaaatcggag aattgaaatt attgattgga taggccaaaa tctgccaatt 360

cctgaacctg atgccatttt tagcattgct gttgctacaa gagatttaga actgttttcc 420

ttagggtaca agattatttt tgattacatg caaagacagg gaatcattca attaaccaat 480

ggagttcgca tggttgtgct aaatcgtcac attagcatgg caatagataa tggtctttta 540

ccttttgttc tggaaacttt aaaacatggt gggaatatac atagagcctt atcttatgca 600

gtaacacaca atagaagaaa aattctggat tatcttattc gccagaaaaa tatagcccct 660

aatacaattg aaagactttt atatctggcc gtgaaaaatc aatcttccag gaaaactttg 720

aacttgttgc tatcttacat aaattacaag gtgaaaaatg ttaaaaagct ggtagagcat 780

gtagtaaatg agaaatccac tcttgtgtta aaaattttat tagaaaaaaa ggaaaatcta 840

gtggatgctg ttttaacaag acttgtaaaa cattctacat atttccaggt gagagaattt 900

atccaggagt tttccatcag cccagaaaaa ttcattaaaa tagctgtgcg ggaaaagaaa 960

aatgtgttaa tcgaggctat ttctgaagat atttgggaaa atcccacaga aagaattact 1020

tatctcaaac agatagtgca caccataaaa tatgaaagtg gaaggcgatt tttggtagac 1080

atcattcaca gcatttacca aagttactca ctaaaacacg aagatattct taaactggca 1140

acattttatg tcaaacacaa tgcaatcacc cattttaaag acctctgcaa atatctttgg 1200

ctgaacagag gaacagaaag taagaaactg tttttagagt gtttagaaat tgctgatgag 1260

aaggagtttc ctgatattaa aagtattgtg agtgaatata ttaactactt gtttactgca 1320

ggagctatta ccaaggaaga aatcatgcaa gcctatgatg ctttagagta gccatgtatt 1380

aacattctga aagtagaata aaatatacta tatactaaaa accaaattag ccatttttaa 1440

ctatcttctt cttaaaaact ctggataaaa atttattttt ttttaatttg ggtagggaaa 1500

<210> 18

<211> 1500

<212> DNA

<213> African swine fever virus (African swine fever virus)

<400> 18

atttctgaat cagtaagcaa tagatagatt ttagaatatg ctgtattaag ttagtttctg 60

aataagtaat taatagatag attttagttt atgtaaaaat gttaacattt gttcataagt 120

tttagatacc attttagagt tactttttta gatattacta ttttagccat tattatctta 180

aataatcact attttagata ggtccccgta ttaaaaacca aattaaccat tatctatgtt 240

tttaataata ctttttaaaa accctccata aaaatttatt tttttttcat aaaagtagag 300

aaaatgttct ccctacagga tctctgtcgg aagaaccttt ttcttccact tgagccctta 360

ggcaagcatg tggttcaacg gctgggatta tactgggaag gccatggttc agttaaacga 420

gtgggtgatt gctttatatg tgtagaccag atttggatgc tatcaatcca taaggctata 480

caaattgcag cctcggaagg aaatgagaac attgtcaagc ttttcttact atggaagggg 540

agtctacaat atgccatcat aggagcctta gagggcaggc aatatgatct gattcaaaaa 600

tattacaacc aaattgggga ctgccatcag attctaccac tgattcaaga tccagaaatt 660

tacgaaagat gtcatgaatt aaatgttaca tgtacctttc aatgcttatt tcaacatgct 720

ataagagata acatgctgcc cattttccaa aaatatggag aagatctgaa tggaaacagg 780

agaatggttc aacttctgta tgagatggca tgccgattac aaaattatga tatcatcaaa 840

tggataggat ctaacctgca tgtttataac ttggaagcca tttttagcat tgcttttgtt 900

agaaaggatt taactttgta ttctttaggc tacatgcttc ttctgggtag aatgagtact 960

gaagatagaa actttatctc aatcataaca cgccatcttg aatacgcatc aaaaaaggga 1020

ctttttgact ttgtactaga atctttgaaa tatggaggtc aagtggatac agtgttgttt 1080

caggctgtaa aatacaacca taggaaaatt ttggcccatt ttattcatga aattccccgt 1140

gaaacggttg aaaagctgat actccatgct gtggagtcac gggcctccag aaaaacattc 1200

aacctgcttt tatcttccat aaactactgt gtgaaccctt ttgtcaaaaa actactgcac 1260

gctgtggtgg aacacaagta catgcttatc ataaagcttt tgctcgagcg gcccaaaaag 1320

aagataaacc tggtagatgc tgctctattc aaacttgtaa aatactctac ttatacagaa 1380

atagtaaaat acatgggtga gttttctgtg gacccaaaaa gggtggtcaa aatggcagca 1440

cgactcatga gagtggacct gattaaaaag atttctaatg atgcatggga agataaacta 1500

<210> 19

<211> 20

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 19

acatacaccg gtcatgccac 20

<210> 20

<211> 20

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 20

taccaatgac ccggagacct 20

<210> 21

<211> 24

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 21

tttgggaaaa tcccgcggaa agaa 24

<210> 22

<211> 24

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 22

tcctgtaggg agaacatttt ctct 24

<210> 23

<211> 21

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 23

cgggggtttt aatcgcattg c 21

<210> 24

<211> 21

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 24

cgggggtttt aatcgcattg c 21

<210> 25

<211> 189393

<212> DNA

<213> African swine fever virus (African swine fever virus)

<400> 25

atataccata ttattgctat tgccatcaat gagaatgcca cgtaggcata ggtcatccta 60

tggccggacc aatccatggc tgcacttaaa aatatcaaaa aaagtttaag ttttgggccg 120

gcgttaaaat ttaaaccttt tctggttgat ctttagccat gtatagctgc gatgtttggt 180

gccttatcta catgctattg gcattcctga tattcgcact aaagtgctat gttacaaccg 240

tcttatgcgt gatttttatc caccttattg gccgaagggc cgccttgtat ttcctgttag 300

gtggtttggc cgtattctac tggtggcaag cagctatcaa taaaatttaa tggctctcac 360

ttaagatcct tgctgtaagc gggcgtttac atactttgat caagaaaaaa aattattttt 420

ggaccccccc ccatgtttta tacaaaaatc atataataaa gtggcgacaa tcaacatatt 480

aatcaaccac agcattttat gatgtgttaa tcaacatata ccatattaat caaccacagc 540

attttatgat gcgtcaatca acatattatt acggagagcg tcaatcaata taatattgag 600

aacagcgact tgataccgtg tatggtggtg gcggcggcat gttgtttgta acagcatttt 660

tcatcattcg aagcttacaa aagatatgta taagatagca tattaatgtt attaacagta 720

atatcaataa ggcgtagcta tagatcttca ctttggtaga ccaataatcc atggttgcgc 780

ttaaaaatac caaaaaaaca ttaagttttg gagggtaaga ttggtttttc accattggta 840

aagattatta ttctaaatgt ttaccccata gatgtgaaac aatgattctt catatattaa 900

catatttttt gacttatact tttcttcatc tagtaaggcg ttaatttttt ccggatctgt 960

cgtttttatt gataaaagag aagagtctgg actgtaattt ttaaataata agatatttat 1020

taatatccaa tttattcgtt tggctcgcta tttccatgct ctcttcgaaa gcatcagctc 1080

ctaaatctat acaaaggaat aagttacctt cacaaaaatt cattaccgag gtaatcattg 1140

cccgattaat gtcagccccc aacataaaac aataatatat agttgtataa ttacaatcat 1200

acatacaggc caactgcatc atttcatcaa tgtctatatt tgtcttctct ttgttataaa 1260

tttcatgaag gtcaaagacg ttgttataag caaccccaca tattaaccgc caatctttaa 1320

aatgactata tcgttgataa aaatattgga tggcttcagt aagcttatat agtatcgcca 1380

tactatacca atacctagtt agcatttcgt tgaatgaaat attatccaat gtaaagttaa 1440

ttgataatgt atctagttca ccaaaaattc ttaatttcag ttgagcatta tttaggaaaa 1500

ggggattatc agataataat tcatggcata gaataatatt actgctagtt ttaacatact 1560

gtacattata aaatatttct aaaattttat tttcactcaa agctttcctc gcacctaact 1620

tttggcatag gtcctggtgc actccatatt gacagtaacc aacccaaagc tgatgtctgc 1680

accccattcg gtaaacagct ctattaaacc atgattgttt tcctgtacag ccttcattaa 1740

tgcaacattt aatgttaaac catgtttaaa acttgctgtt tttattaata tttgttcatc 1800

tatacaagta tgataaatcg taattggggc ttcatgccac cacaaaccac aacgctctaa 1860

aatacaataa tcatctttta acacaggctg tgtagctagt acttttttag taagtgcttg 1920

taaagtagat ggcatcttct atctgcaaaa taattatttc cgaaaaaaaa atcaaattaa 1980

aatactaaat tctatttttt ttttaataaa gcctgtaaat tatataataa atctcgccca 2040

ccgtattatt tccggacaca actttttata cctcattata tttttagatc tatagttttt 2100

taacaaggca ttaatttttt ctggatctgt cgtttttaaa gataaaagag agacgtttga 2160

actataataa tctttaaatg ataatatttc tactaatata tcatgattct tttgttttgc 2220

taattctaag ctctcttcga aagcattagc tcctaaatct atacaaaaga acaagttatt 2280

catataaaag ttttttaccg aggtaaccat tgcccgattg atgtcagccc ccaatacaaa 2340

acaatagtaa atggttaaaa aattgctatc tctcatacag gccgatatat catttcatca 2400

atattcatat caaccttttt tatatgatac atttcatgaa gatcagacac gttattaaaa 2460

gaaagcccac atattagccg ccaatcttta aaatgactat atcgttgata aaaatattgg 2520

atggcttcag taagcttaca tagtatcgct atactatacc aatatctagt tagcatttcg 2580

ttgaatgtta tttcattcaa tataaagttg atcgatatct tctctagaaa acaacaaatt 2640

attactttta attcctctat attctggaaa aggggattat tagataacaa tttatggcat 2700

aaaataatat tactactagt tttaatacga tgtattttat aaaatatttg tacaatatcc 2760

atttcattca aaatttttgc gcctaactcc cggcagaaat tccaagtatg ctccgtattg 2820

acagtgacta agctagagtt gatgtctgca ccccattcag taaacaactc tattagatca 2880

tagttgtttt cctgcacagt tttcattaat gcgagattta actctaaacc atctttaaaa 2940

attgctgatt ttatcatcaa ttgattatcc tcattagtag aaagcataat tggagctcca 3000

tgccaccaca aaccacaata tttcaaaata aagtagtgtt ctttagatat gtgctgtgtg 3060

gccagtattt ttttagcaag agcctgcaga gaaattggag tagacatatt tttttttgca 3120

aaatggttta agtttttcaa gaatacagat tggataaatt aggttgttga cttagttaca 3180

ggaggtatta aatattatgt agacataaaa atgagatcct ccaaaaaaat aaacaacaaa 3240

aaaaaatatg tttaatatta aaatgacaat ttctacattg cttattgctc ttattatact 3300

acttattatt attttagtag tgtttttata ctataagaaa caacaaccac cgaaaaaggt 3360

ctgtaaagta gataaagatt gtggtagtgg agagcattgt gttcgtggat catgtagctc 3420

attgagctgc ttagatgccg taaaaatgga caaacgaaat attaagatag attctaagat 3480

ttcctcatgc gaattcactc ccaattttta ccgttttacg gatactgctg ctgatgagca 3540

gcaagaattt ggaaaaacac ggcatcctat aaaaataact ccatctccaa gtgaatccca 3600

tagcccccaa gaggtgtgtg aaaaatattg ttcatgggga accgatgact gtacaggttg 3660

ggaatatgtt ggtgatgaaa aggagggaac atgttatgta tataataatc cacatcaccc 3720

ggttcttaaa tatggtaagg atcacatcat agccttacct agaaatcata aacatgcata 3780

aataaataca ttaggctcat cgtatctttt taaaatccat aaatattcgt ttgatatatg 3840

ctgaaatttt tataaaaaaa aataactatt tcctataaat catctagaaa tagtcctcgt 3900

tttgatcggt ttatatctta taatattgtg catcgatgca caactgcttt ttttggtcct 3960

tctggaacat cattatattt tctttcatta atataccatt cagatgtaaa cgttgaataa 4020

tttttatggc aacaatctac cattgaatta tatttagtaa catctaatac atcgtttgtt 4080

ttatcaggct cagctctata atcttgataa tttttgttat cagcttctaa agctccatca 4140

ttatttttca aagaagtatc cataattatg tttggtaaaa atactttaag ttttaatgtg 4200

atatttaaaa tggttgttat ataaatttac cgcttacagg taatctttat tcagtgtcat 4260

aaactatact tttgatgatt cagtattttg tgaatcagta catttattat cattaatatt 4320

tttaggctgt ttttccaatg ttttattgtt gcaatgagcc tgctcctcct ttgacgagga 4380

agtgtctgtt ggagtcatct gtttaggaag agtatcatcc atatctatta tgaagaaaat 4440

atataaatat tgatatacaa tcaaaaatat ttttgatcac gtctttgtta tctatcgata 4500

ttgttgataa cgtcttgaat aacctacatc atttttttac ataaaaaaat agatataatt 4560

tttattatat ctcaattatt ttaaagataa ttatcaatac agcaaatatc ataagctaac 4620

atatttttcg aataatagtt ttttagtaaa gtattaatct tttcaggatt ggtttctttt 4680

gataataaga taggattcgc tttataaatt tttaaagata atatattcac aatgatagaa 4740

taaccgtata tatctgctaa tgtcttactg tgttcaataa cattagcccc taaatccata 4800

caaaagaaca tattttcaat acaaaagttt tttaccgaga ttaacattgc tcgattagcg 4860

ttggctccca atgcaaaaca gtagtaaatg gtcaaaaaat tattatcgcg catacaggcc 4920

agctccatca ttttattaat actcatatga attttcgttg tgttacatat ttcatgaagg 4980

tcaaacacat tgttgaaaga aagtgcacaa attaatcgcc attcatcaaa atgcctgtat 5040

tcttgacaaa aatattgaat agcttcttta agattatatt ttaccgctat gccataccaa 5100

tatttggtta gcatctcact aaatgagatc tcatttaaca tagaatttgt tgttaaatcc 5160

ttcaactccc aataaatgat catccttaaa tccaccatgt ttacattttg taaaaaaggg 5220

ttattagaaa ataattcatg acacaaaatg acattactac ttgttatttt acactttgtt 5280

tcaaagaaaa atcgtaaaat ttcacttgtc tcaagctctt ctttagctcc caattttcgg 5340

cataggtttc gagtatgctc gttattaata aaaagtaacc cataattaat atttgcaccc 5400

cattcagtaa acaacatgat tagatcatca ttgttttcct taactgccaa taccaatgca 5460

gtattaagcc ttataccctc tttaaagcat aatgtcctta tcattatttg attatcatca 5520

tctatataca ttgagatagg agcttcatgc caccataaac cataacgctc taaaatataa 5580

taatcatctt tagatacgtg ttgcgtggcc aatgcccttt tagcaagtgc ttgtaaagtc 5640

gatggctgca tgtttattct gttaaaaaaa aatcaaatta tcgggtaaac ataaggatca 5700

acccgtagtt aatatttgca gtagtatttt ttaacaatga attataataa aaaaataatt 5760

cattactatc tattataaaa cccatcttta actttaaaga agaactagat catctttttt 5820

ttgttgtgtc agaacttctt caatttatta cccacatttt atctaaaaaa aataaaaact 5880

acatcatatc ttgtttcttc atcaaattat cataccattt atagggtgta ggttgggaac 5940

attccatcat gtggtaatca gggtatttat atattttttg atagtaacat ctatttggca 6000

gatgtattgt ccaacaatca tgtctaataa aatcattttc acctatgggg gaatcatctt 6060

aaaaacctta ttcctacaga ttccattttg acagtcctag caaaagtcac aatattttcc 6120

atgagtacac caatgttcaa gctctctttc gggaggaatg ctgccaattt tatgtttttt 6180

agcttctaac tctctgtaca acatcagttg ggaaagcaga aagaagatta ccaggagaac 6240

cattaaatat ataatagtct gcaaactacg tttgcgaatg taatttgcaa ctaaaacaca 6300

acccacaagg taaaatccat aagttaataa cttttgccat tttcgtatga cagcctcgtg 6360

ccattcatgg ttgtgttgtg ggcattctgt tcggtaaact tcatgaggct ttatagaagt 6420

tacatagtag gtacagaatt cattgtgacg aaaaacactg cagttagcta tgtagtcatt 6480

ttcaagaatg ggagaatggt tttcaaagac cttattctta cagatgccat cttgacagtc 6540

ccaacagaac ctacaatgat ttgcataggt gcaccagtat tcaagctcct tttcaggagg 6600

ggttcttgtt agatccagga gctctagctc atatgtataa agaagagttg gaatggatag 6660

taaagtaaat atttgcagac caagcatggc tacttgtgaa caagtggctg ctcgtcaaca 6720

aatagctgtt tatcagcaaa tagctgttta tcagcaacaa ctaattatca gcaaatgctg 6780

cttgtgggta agccaataaa taggccatac ccttgaaagg agaattcagt ttgataaaaa 6840

aaataacgag ttttctaata acccggtcaa gcatttaata aatgaatagc atcacacgtc 6900

tgcatcgtgc attctgcctg gaaaatgggc ccatctctaa tatatttaca ctgacggtga 6960

atcatacagt gttccatggg atagctatgc tcctgtacag gaggcatatc ttttagaact 7020

ttattcttac aaagaccatc ttgacaagcc cagcaaaacc gacaattttt cacatattga 7080

caccagtatc taagctcctc ttccagggga ttgtcggtcg aaaacccctg tagactagct 7140

aggccagcta gcagcaagcc gaggtaacta aagaacctca ttgtagtgtt atattacgaa 7200

aaaacatgtt aaaatttgga aaaaaaagcc ctttttatag atctggaaaa aaattttcac 7260

aaatctaatt aaaagcctta cagatcatcc ttttcataaa ttttcattaa caattggtgg 7320

gggcggttgt gaggtactgg atcagaacaa tccataacat ggtaatgtcc atttccttca 7380

ccatatgtac actggttata ccagcgagaa acctcacaag atgtcaaata actgttctca 7440

acaatcaatg gcatgctctt attcaccttg ttcttgcaaa ttccatgtgc acattcccag 7500

caaaacttgc agttttccat gtaagtacac cagtatccaa gttcttcttg tggaggatta 7560

tccgttgaac gaagatgccc tcctgcctga gtaggtagtc ctaagacctg attggccagc 7620

aggccaagaa tttccaagaa gatcaccaac attgctacgg ctggctgaac agctggcaga 7680

tagctagcta attagcaaac caagtgactc gccctctcta ctcttaatat gagaatttaa 7740

gattcggtcc ggcttttttc ccatgtttta cagggaaaag gtatttttag cctatgaatg 7800

tacatggttc cgcacattaa aaaaaaataa aagaaattat ttaatattgg ctgttatttt 7860

ctttcaacta gcaacaagcc aggtaactaa agaacttcat tgtagtttta tattacggaa 7920

aaggttaaat tttggacaaa aaaaatcata tctaattaaa aatcctcaca gatctttctt 7980

ttcataaatt ttcattaaca attggtaggg gcggttgtga ggtactggat cagaacaatc 8040

cataacatgg taatgcccat ttccttcacc atatgtacac tggttatacc agcgagaaac 8100

ctcacatgtt gtcaagtagc tgttttcaat aatcaatggc atgctattat tcaccttgtt 8160

cttgcaaatt ccatgtgcac attcccagca aaacttgcac ctttccatgt aagtgcacca 8220

gtatccaagt tcttcttgtg gaggattatc cgttgaacga agatgccctc ctgcctgagt 8280

aggtagtcct acgacctgat tggccagcag gccaagaatt cccaagaaga ctaccaacat 8340

tgctacggct ggctgaacag ctggcagata gctagctaat tagcaaacca agtgactcac 8400

cctctctact cttaatatga gaatttaaga tccggtccga catttttccg atattttaca 8460

agaaaaagat atttttagct acaaatacac ttcatatatc cctaaaaaaa acaaaaattt 8520

atttaatttt aactattatt ttctttccac tctctcttta agattttgta aggattccag 8580

ggctttggtt cagaacaggc cattacatgg tgaatcccct gtcctagatc atacatacat 8640

ttatttagcc agcgggaaac tatacatgat tgcacatact cattttcaag aattgttgta 8700

ttctccaatt tgccctcaca aaggccattt tgacaattcc agcaaaactt gcagttttct 8760

gtataagtgc accagtattc aagttcttct tgtggaggat tatccgttgg atgaagttgt 8820

ccagctggtt gattaggtag ccctaagacc tggttgcaat tcatggtatg gtagataccc 8880

ttatctaaat catacataca tttatccagc caacgggaaa ccagacatga tttcacatac 8940

tcattcttgt aaattactga cccatctatt ttgtttatac aagtgccgtc ttggcagtcc 9000

cagcaaaatt ggcaactttc catgtaggca caccagtatt cgagttcttc ctctggaggc 9060

tcctctgttg gacgaagttg tccaacgagc tgacttgaaa cctggctggc cagaaggcca 9120

agaattccca agaagatcac caacattgct acggctggct gaacagctga ctgaatagct 9180

agccaattag caatccactg tacttttcat aagatcattt aagattcggt cggcattttt 9240

tcaatagttt gctaggaaaa aatttttaat tttatagatt cacactactt cattctcatg 9300

cttaggaaaa aaacaaacta aatcttacaa tgtatctgga tctaatgaga agctagaatt 9360

catctttttt caaatccttt ctgggatgtt cattcttttt ccactccttc cttgcaattt 9420

tataaggatt ccagggcttt gggtcagaac agttcatgct atggtaaatg tgctcctcca 9480

catcatatct acataggtca ccccagcggg aaacctcaca atattttaca tagtcattct 9540

caataatact tgtggagttg tttccccaaa ccctgctggt acaaatccca tcttcacaat 9600

cccagcagaa ccgacagctt tccacataag tgcaccagta tccaagttca ttctctgggg 9660

gttcaaatgt tagaggaaga tgtccaccta cccgagtaga agtggaggat gaaaccaggt 9720

tgctactggc agcaggccaa taattcccag gataatcacc agcattgtgc tcaaccagca 9780

acggctagca acgactagca actgactagc aatagctaga aatggctagc aatcagtagt 9840

agctaacgct ctactcttta taagaaaatt taaaattcga tcagattttt ttagaattga 9900

gaatgagtaa aacgcttata ttctttttct agctagaaaa aataagctag tttaagatag 9960

gatttccctt actaacggtt taatttttag caaaggtata ggtaaaatac acttgtactt 10020

agctgcaaaa aaataagctt atggcgtata agccgccata agtttattta attaaaatgt 10080

taaactctgt gataagactg gaatcttagg caggtttgat gtggagaaca gcatgaaata 10140

caagagtgcc tgttacacga ataagttctc tcaaaccggg gatggtcata ctcacatcta 10200

tgaaatcctg gtctaggaga ttcatttgat gcatgatggc cgcacccaca cttatgagac 10260

actgaagaac taaagggttt aattttgatc tgaatggtac tatataggat gatggcaatc 10320

catatcaaga ttagagcaat caaaatcacc tcctcaagaa gcatgatgta gccttaaatc 10380

ttagactgct ttaaacctta ggccctcact atctttaatg aaggagttta aattttgatc 10440

cctttttcaa gacccattta gaagaaaaaa ataaagttta tatcaatcta attcataagt 10500

catctctttc ataaatcttc atgtattctc tatgtggata agtatgggat gttggatttg 10560

cgcagtccat ttgatgatct gtatggtttt tgggtccttc ataataacta catataccat 10620

tccagcggga aaccgtgcaa tttataatcc agtcattttg atgaataact ggccaatctg 10680

tttgaatcct gtttcggcag ataccgtgga cgcattccca gcaaaagtca cattggtttg 10740

cgtaagtgca ccaataaact agctcatgtt caggaggata acgggttggt agtaaatctt 10800

ctaatttacg tataggagcg gcttgaagga caaccacccc cagtagtact agaatcagta 10860

cctttatagt ggccacccta cactagacct ctaagttgaa gacaaagaac taaaatttag 10920

agccgtttaa ttactactaa taattatatt ttttattgtc tacaatagga ttctattaaa 10980

aaataatgat ttttaccaag aaatattttt ataaaaaatt aatatatttt gtaataaact 11040

ttatttccaa tgactgttaa aataaggaaa ctatccttag ttagtcgagg aagatggtta 11100

ggttatttcg caatccgata aaatgtttat tttatcgtag gtctcgtaaa atccaggaaa 11160

aaaaattacg gaagagttta aaaaagctaa atttttacca ccctccagaa gattgttgtc 11220

aaatatatcg tttgctagaa aatgttcctg gaggaactta ctttattaca gaaaatatga 11280

cgaatgattt aattatggtc gtaaaggatt cggtggataa aaaaattaaa agcattaaat 11340

tatatcttca tggaagttat attaagattc atcagcacta ttatattaat atttatatgt 11400

atcttatgag atatacccaa atttataaat atcccttaat ttgttttaac aaatattata 11460

acatctaagt aaatattctt ggaatggatt ttcttataga atggttacag gatatgtcag 11520

cgacaggctt aataacaaat ttgttaatat ttttttgtta aataaatgaa caggccacca 11580

tttaatatta cccgttgcaa aataagaaaa aaaaacaaac ttatagttac aaatcatctt 11640

gattaatcac atgtcgtttt aactcaatga accattctaa atctttgggt tgtgaacaat 11700

tcatgttatg ttgatagtgt atcctaaagt gagcttcata catacaccgg tcatgccacc 11760

gggaaactgt acaattaaca atataatcat tttgcgtaat aatagggtgg tcactaaaca 11820

ctttattttt acacattcca tctttacagg tccagcagaa gtcacagtgt tttgcatagg 11880

tgcaccagaa cttgagatcc ctttcaggag gcctacgcat ttgcatcgga ttatctgtgg 11940

aaagaggtag gttcattatt atgttcgtca tcaaaattcc taaaagaaca tagaagccaa 12000

gaaagataag cagtcttgta gcggcttgca ttcgcattcg tgagtattgt ttgcgaacat 12060

agcttatgag agcaatggta gctatcatac aaagacaagt atgtttgata ttctcagtgt 12120

caatgaccct atcctccttt atttgcatta actcatcaaa ccaatcataa tatgtgggat 12180

ttgtacagct catgatgtga aagcggcgta tcctagagtc tgtaaagtag ctacatcttt 12240

cattatagcg agaaacccta catatttgta tgtaatcatt ttttttgatg agagggtgtt 12300

tttcaaaaac cttattttta caaaccccgt gtcgacaatt ccagcagaag tcacacgatt 12360

ttgcataggt gcaccaatac tcaagctctc tctttggagg tctccgggtc attggtaact 12420

ctcctgttcc tggaaaagat tggctttgaa tgaccggctg catgaccgcc agtaccaaaa 12480

ggaacacaat caccttcatg gctgcaactt ataagttgca acttatgggt tgcaatactg 12540

caacgtatag gttgcacctt atagatcgcg actcaaaagg tatgaaaacc ttaccctcaa 12600

tacagaattt aagttttaat cctgataatg tatctgttta tgaaaaaaaa ttttttttac 12660

tcatgtatga attcttatac gaatcataat atgtaggctg agaataataa ttcatatacg 12720

gtgttgcggg ctcaataaaa attttgttac cacaaaaaat aaatgctgga tttttaagat 12780

atatatctat taatgactaa accctttata cgctgtaggc tgaaaacaat ccatataatg 12840

aatatacggt gatttgggtt taataaaata catacaacgg tcaaaatagc gggcaatact 12900

acattgacta atataatcat tttgtttaat aagaggcata tcatcccaca ctttattttt 12960

acaaataccg ttcctacatt cccagcagaa atcacagtgt tttccatacg tgcaccagta 13020

ttcaagctct cttataggag gcgtataagt ccttggtaaa ttttgtttca tataaaagat 13080

ggaaaggggt cgatttaaac ccggctgaga tagccaaatc aaaatacata aaagagcaag 13140

tagtttcata gtggtattta gatgtaaatt tttatagtat gcaaatacaa tgtaacctac 13200

aaatacaata ctaaatacaa ggtaaaaaca acaatgtctt ataatgattg gccaataatc 13260

accccccccc cccccatttt tccatgaata tttcatttcc tgtatagggt ctaggatgtg 13320

aacactccat gttatgatga ttaggcattt taactgatat ttcataaaaa cacccccagg 13380

aattgcgatt aactatacag tttacaatcg aattcatcga attagactca tttgttatct 13440

tatttttaca aatgccattt tgacaatccc agcagaagtc acaattcttt acatacgtac 13500

accaatatgg aagctcctcc ttaggaggat gctgggttct tggtaattct ggtaattcat 13560

gtgcaagaat gaggactgag tagcccaaca aaagtcctag aaccttcatg ttgtgtccaa 13620

atggcacctg tcattttaaa aaagatttaa attttgctac cgcaaaaaaa aatccagtat 13680

gtattttttt aatacatata attattgaag tcttataaga taaagccgag aacactatat 13740

tttgtataga tgatgtatcc ggtattcaaa ctctcttata agtacatgta ggaaatggtc 13800

aattattcaa gattggctga gataacaaca aaaccaaaat actcaaaagc ataagtaatt 13860

tcatggttgt actcagtcgt agatttttgc agatcgcaaa tgcaacgcaa ccagcaaata 13920

caaagctaaa tacaaggtaa aaacaataat accttataat gattggccaa ttcttatccc 13980

tccatttttc catgaacatt tcatgttcat aaagtctagg atacgaacaa catttcatgc 14040

tatgatgatt aggtatttta agtgatattt cataaaaaca ccacggggtt gttggtgatt 14100

gataggtaag aataaggatg gttgaataac ctagtaaaag tcctagaaaa accttcatat 14160

tgcgttcata ccacagatgt tatttaaaaa aaatataaat tttacagtat gtgatataca 14220

cataccacaa aaatgttctt atattaacta aaatatgtgg gcagagagca attcatataa 14280

tgaatatatg gtattttagg ctcaataaag tacatacaac gatcaataaa acgggtaata 14340

ctacatttac tgatgtaatc attttgaaca ataagaggca tatcatccaa aaccttattt 14400

ttacaaatac cattcttaca atcccagcag aaatcacagt gttttccata cgtacaccaa 14460

tattcaagtt ctctcatagg aggcgtatag gtccttggta aaatttgttt cgtataaaag 14520

atggaaaggg gtcgatttaa aactggctgt gctaaccaaa ccaaaatact caaaagaacg 14580

aaaagtttca tggttgtact cagacgcaga ttcttacaaa gcgcacatac aaagcagcct 14640

gtatatgcaa taccaatgat gaaatagaga cagtattgct ttatagataa ttgttgatgg 14700

tcaccccccc ccccccccca tgtttgcatg aatatttcat ttcctgtata gggtctagga 14760

tgtaaacatt ccatgctaaa gtgattaggc attttagatg aaatttcata taaacaggat 14820

tgagtcttgg aatcacggaa aactctacag tttacaatag aatgattgga gtcaatgaaa 14880

cgagattccg ttatcttatt tttgcaaatg ccatcttgac agtcccaaca gaaatcgcat 14940

tgtggtacat acgtacacca atatgaaagc tcactcttgg gaggatgctg ggttcttggt 15000

aagtctggta attcatgtgc gagaatgagg actgagtagc ccaacaaaag tcccagaaga 15060

accttcatgt tgcgtctaaa tgacacctgc acttacaaaa aaaaatttaa attttgaata 15120

taacacaaaa aaaccacctt aaaatttctt atattatttc ttggatctgc cccgacgtca 15180

tacaatgtat taaaattata gaccaatcat ctttttgtat ataggctaat catctttata 15240

tatagatttt agatgtttgc ttgttgtatc aacttaactg ctagcgaaga aaatggataa 15300

aaactttctg tatttttata ggttgaaatc attttatgca catcgctagg atctaatatt 15360

ttattttgaa gaaccgaatg tgggcttaaa atttttttct tagaaaaaag tagaatcata 15420

atattgctat gtttttgttt aatgatttct tgtatctttt ttgtatacgg gttggcaccc 15480

aaacctatac aaaaatatac attactcaaa taactacctt ctatacataa tcttttttcc 15540

ccacgtattt tcctatttat ttccctattt atggaattaa aggatatcaa tctctctaag 15600

gcacggtcaa ggtctgcgcc taaggcaaaa caataatata tacctaattt attcccaggg 15660

cgtgcacagg caagaaacat catgacgttt agccctaaac gtatattttc ctgaaaatac 15720

gcatgatgaa cttcatcaat attacctaag tatatggccg tttgtaaacg ccaaagatct 15780

aaatgaggaa attttttact aagataatga ataggttttg tgagattaaa atctatggcg 15840

aacttatacc aaaattttaa tacaagtgta tttctcgtca tttcttcttc tttttcatct 15900

aaatataaga taaaacgatt gtaaacaaag tctatcaata ggtgaaaatc attgctatta 15960

aagctgtcga gaatcaaaat attgtcataa taaatttcga tcgccagtaa aacctttttt 16020

cgtttgacga gataaacaaa catattatac aaccctacat ctaaaaattc tggattggct 16080

cctagttgga tacacaggtc tttagtctgc ttcgttttgg cacacatgat gccaaaatta 16140

atatcagcac cccataaaac aaataacttg attagatcag tctggttttc cttcacagct 16200

tttactaagg ctctgtcaag ctcatagctg tcgacatcag agcatgacat agagccaccg 16260

gttaccattt tacattgctt acaaaaacct atgggtccgt tttcccacca tagtccaagc 16320

tgttgtagaa taaaaatatc atcctcatga taatttgaaa aagccttggt ttctatcaag 16380

actttttttg taagaacctg taaagagttc atcgtattat tatgaataac aggagtaaac 16440

gtaatcaatt ataaaagtga ttttttcgaa aaaaacttta gatggttgaa aatgataatg 16500

tacatgttca tacaaaaaat agatgcagtg atgtctaaaa tcaaaattta attttctatg 16560

taaaaagtac agacttactt atttgggtta aattgtttat tttaaacttt aattaaccgt 16620

ttgagttagc gatgtttgat ttatcttcca tactcatccg gggggggtcc ttatagctct 16680

gacattattg tggattattg aatataatga atacttcata gatgctaaac attttaatag 16740

tagttctgag gcttaattgt actctataaa tttataaaaa ctttttgatc aaaatttaat 16800

ttcttataaa aagagtacag acgtcgcttg tttaagcttc atcatgtttc attcattact 16860

ttctacaatt acgggggggg ggagtcccct catagcttta gtattgctat ggtttactaa 16920

ttattatgta gaatttatag aagcatatgt acctgaaagt atacctactc tataaaatta 16980

aataatttca gtatattttt tttatgaata gaacggaaat gatataaaaa taatttaata 17040

ttgcaaaaaa aattcataat gttggtatgt attataaaca taatagcatg tgtaatttat 17100

aaactgactc ctctatataa ttattagatg aggtaccaac ctacttatga tatgccgatg 17160

atagatattg tatactataa aacaaaatta ttttaaatgt attcatggat acattataac 17220

atttttaccg caaattgtct ctcagcgaag aaaatgaatg aaacgtttct gtatattcat 17280

aggttgaaat tattttacgc acttcactag gttctaatat tttcttatga agtattgaat 17340

gggggcttaa aagtcctttc ttaaaaagaa gtttcatcat aacattcttt tcttgtctaa 17400

gaagagtttc ttgtattttt tttgtataag gattggcacc caaacttata caaaaatgta 17460

cattactcca aataccataa tttgaaaaga aagttatttc cctatttact tcatgattaa 17520

tgaaacctat caacgtctct aaggccgtat tgatatttgc gcctaaggca aaacaatagt 17580

atatacccaa tttattttga gggtacatac aagcaagcga catcatgtca tttggatcta 17640

aacgtatatt ttcctgaaaa tatgcatgat ggatttcatc aacattacct aagtatacag 17700

ccgtttttaa acgccaataa tctaggtgag gaaatttctt actaagaaaa cgaataggtt 17760

ttataagatt aaactctatg gcgatcttaa accaaaattt taatacatat gtatttttta 17820

tcattttttc tttttcatct aaatttaaga taaaacgatt gtaaataaag tctatcaaca 17880

cgtaaaaatc atggctatca aaactgtcga gaatcgaaat attgtcataa taaatatcta 17940

tagctaataa gaccttttgt tgtttaatta gatcaacaaa catattatac aaccctacat 18000

ctaaaaattt tggatcagct cctagttgaa tacacagaac tttcgtcctt tccgtcttgg 18060

cacatatgat gccataatta atgttggcac cccataaaac aaataacttg attagatcag 18120

tctggttttt cttcacagcc ctcaccaagg ctctgtcaag ctcatagctg tcaacatcag 18180

aacatgacat agagccactg gttaccattt tacattgttt acaaaaacct atgggtccgt 18240

tttcccacca taatccaagc tgctgtaaaa taaaaatatc atcctcatga taatttgaaa 18300

aagccttgtt ttctatcaag actttttttg taagaacctg taaagaattc atcgtattat 18360

catgaatgaa agcagtaaat gtaatcaatt ataaaattga cttattgaag agaaatgtta 18420

aatgagtgaa atcggtgttt atgatgatgt acatgatcat acgaagaaac acgttcactg 18480

gtgtccatga tcaaaattta atgttttacg taaaaagtac agatgttaac tgtttagttt 18540

aaacataaat ttaaccttta gtttaaaccc tagttaatga tgtttaatat ttcttctata 18600

ctcattcagg gaagtgtaat gattctaata ctgttgttat ggattattaa tgaaaacttt 18660

acagatgctg gagggaataa ttttaatcat actgttttaa tgtagctata taagctttca 18720

tcaaaattta attttttttt ataaaaatac acgaattaaa ctaaagtcta aactttagtt 18780

tgactatttg agttaatgat gcttaactta tcttccatgc ttatcaaggg ggggtcctaa 18840

tagttttgat actattgttg tggattgttg aatataataa atactttata gatgctgaaa 18900

tgtttgaaaa taatagtaca tcaatgttgt aagtttgatc aaaatttaat ttctcataaa 18960

aaaggtacac atcaacattg ctcatttaag tttcatgatg tttgattcat tacttcctac 19020

aattactggg ggggggtctt taatagcttt agcattgtta tggtttgctg actattatgt 19080

agaattcata gaagcacgtt tagatagtaa tatcactgca gtgtagatta tgaaatacat 19140

actaaactaa tttcagtata ttttttttgt tcatataagt taaggtacaa aaatgattaa 19200

acattgcaaa aaaagaaaat cacaatgcta ttatacatag tgatcatagt ggcttgtatc 19260

atttctaaac tagttccaaa tgaatattgg gcaatacatc tattttttat cattatgatt 19320

tttatggtat atatgtatga aaagttagat atacatcaaa aatctcagtt ctggaattat 19380

accatgtcag gcttatctgg acataacgta caggtaacat gtaagtgtta ctaaatacta 19440

tgaagtatct attttttttt gttgtaaaaa aaagaacttg atagtatttt ttaaaaaata 19500

aaataattaa ttgtacgtca acttccttat tttattcttt aaaaataact cgtaagtatt 19560

atttatctat tttttgaaaa aatagatgta atcggtttca tcatttaggt gtgtatttct 19620

ttttagcatc tatcaagaat tcattgttta gtgatatgaa aacaatgaat gatcattatc 19680

ttctatttaa caaccaccta aataaatgaa cgtctttttc atcttaactg attaccaaaa 19740

gttattttgc gaaaaggcat acatatgatc aatatcagac ctacaatgaa tatttccata 19800

atatcccttt attgtaataa ttctattttt gcattccgat atctcatcat ctgtgctatt 19860

atatgtttcc ataactgttt catcatcaaa cataaatcct gttaaatagg caaaagactt 19920

taatcccgga tagattttta ccattttcct gagagccgtg tatagcttgt aataaatggc 19980

caaaaatatg caataaagcg tagaaagaga gtaatttttg gcataaaaga ttttgaaggt 20040

ttgatgaatg gctaaatcgc atataatata agatacgatt ttaaagcgca cctgttcacg 20100

cagatttgtt gaaaaattcg tggaaagatt taacaaataa aaggttatta atagttgctc 20160

atcattcccc ttatacgaca tcgtcagacg ctctaatatt ttactactag gcacatctgc 20220

cacatgttga acatttaaag cctgttcttc ttctgtgtta cggcaaaaga gccgtgcgta 20280

ttcaggtgaa gctccccagg ataacaacgt ccttgctacg gctaaatttt ttttgacgat 20340

gacttttatc agaaataagt ctttattttt gcattgatca ctatgcgaat ttgtatagtt 20400

gacgccgttg cattgagtac attgatataa tgttttacaa ttccagcgta gccctaaatg 20460

gtataaaaga actgtatttt cgacataagc atgctgatta acgatgtttt tgagacaaca 20520

cgtcgttaag gacaccatat tgtctccaat ttgttagata aaagtcttta ctaaaaaaat 20580

agatttttag ttttaacaat cgagatttta ttatttggat gcatcatcaa aaagatttat 20640

aagtataaga ggttgtataa gaaaaaaaat gatgttatac tatttatgtt aaaatttaat 20700

ttatcatata aaaagtacag atttaatcag ttggttaaac tatttagtta attaaactaa 20760

atagtttaac catttagtca gactacttgg ttagcaatgt ttgagctttc ttccattctt 20820

atccgggggg gggggtccta atcgttctaa tactattgtg gatagttgaa tataatgaag 20880

actttataga tgctataatg atgaattcta gtatgcctgt ataaaataat taaccttttt 20940

gatcaaaatt taattttttt ataaaaagct acagagtagt gttttattaa acgtggctta 21000

tttaaaagtt acacaatgtt aaaatctcta cttactttaa ttctttgtgg ggttttatta 21060

actttatcca tattatggct tactacttac catgtagaac ttatagaggc aatagatgat 21120

ttctacgact gaaatataga atagtccatt ttctatttgt aaaataatga tttatattct 21180

ttcctaaaaa tgatacttta tatggtttga aaacaaatat taacaacttg attttttttt 21240

ctataaataa actataaatg aaaatagtaa aactcataga gtcttataag tgaacatctt 21300

cataatgtta ctcaaacgtt ggactattaa aaaatattcc gtgtgcatta ttgcttttaa 21360

tcagtatgat tactttatac gaagccgcta ttaaaacgct tatcacacac cgaaaacaaa 21420

ttttaaaaca ccccgatagc cgtgaaattt tactagcttt ggggttgtac tgggataaaa 21480

ctcatattct tgttaaatgt cgtgaatgtg ggaatatgag tcttaccgga aaacacagta 21540

caaaatgtat taacattaat tgtctactta ttcttgccat aaaaaaaaag aataagcgta 21600

ttgttgatac cttgatagga atgggcgcgg atgtaacata tatacatctt ttaaagaata 21660

agataaaact gtcatacaac cagctgtcta tgcttaaaag caactcgcag atttcattga 21720

aggagcttca tgctatatgc tatcttttat atggtcggct tcccaaaaaa attaaacaag 21780

ggatgcgact gtgtaaaaca atggcgggac tatgtggtga acttttatgt gcatttttag 21840

ctccgtaaat gataatatgt atttaaaaca aacagatatt accaaaatat attctatgta 21900

cataatatct gggaaattat ttttttttct cataccctta aatataaaaa tattgggttt 21960

cttcactaaa ctttagaggt aaaaattttt ctttgttttg caccatcatg tatgggttta 22020

ggctgtccca gggattgttt atttgaatat ttcctaaata ggaacacaac gccatgatca 22080

tatatctttc attctggtaa gctttttgat acatcttcaa agatgccgta cctccgagtg 22140

tgtaacagca aacaaacgtc cgtacttttc catgggtcgc agcccattcc attccgtagc 22200

tcagcatctt ttgctgtatt tttttattcg ctttataaaa aaagtttttc atccattcca 22260

cgttctcata aaaacaggca cttaaaaaga gcactagggg tagtgtagtc ttattataga 22320

atgtaggaat gtatgtttta gttatttttt tcaacgcgtg ttccatacta tgttttaccg 22380

ccataaaaat acaaaaccaa taccaacttt ttctataaaa ggttttgctg tacacatata 22440

aacgagcaaa atatatttca aactctatat tctttttata aaaaaactcg agacagtcgt 22500

ttatgttacg actttttcta aatacctcaa aaacagtaat taattcactg tcgctgtgga 22560

aatgttcgta agctaactgt ttaatgtctt taggggtcaa ttcttttttt gggagcagtg 22620

gtttgagatt cggcaaaggt cgtctaaagt agtgagcgaa cttttcattc gctccccaac 22680

acaaaagccg ataagccagc atgtagttat cacgttttac cgcgtaaata agcaaatagt 22740

ttatattgat acatgtacca tgttgctgcc cgtttggaca tatgttgccg cattctgaac 22800

acttatgaat gagatcatag ttcttacaac ataaccccaa acgggttagt acttctttgt 22860

cacgttttaa aaactcgaca tgattcttta atgttaatgc tttgagcgca atgttaaata 22920

aactctgcat tttattaaaa tgaggttagt atcatgtttt agtataaaat ttagcggctg 22980

tttacataat gctaaataaa cttaacgttc ctactaaacc aaaaaaaatc aaattgacta 23040

agtcatagag aatttgacga tgttggtagg taatttttta acatggtata tattttttta 23100

gggtcggtta tattaggtaa taaaagagga cgtgccgtta aagtattttg cttaagatcc 23160

tttagatcct tacaaaaata tagattgttc gtctgatgat gccactgtgt tgcagtgatg 23220

gcttgatcaa tatcacctcc caagacaaaa cagtagtata tcgttaaaaa gttgtaatct 23280

ttcatacaag ccaactgcat cattttatcg atgtccatat gaacgatctt ttgctcgtat 23340

atttcatgaa ggtcaaatac attgttgaag taaatggcgc acatgagtcg ccacatacta 23400

aggtgcccat atgtttgata gaaaaaggag atagctcttt taagcttata ttttactgct 23460

atggcatagc agtatttaac gaatacgttc atgggtacat tatctaagat ataaaatatg 23520

aaaaacttta actctcgatg aatctcttcc cccatttcct gtacatttag agcttccaac 23580

ataggatttt tatcaaatat ttcatgacat aaaataatgt tattgctcgt tttatgacgc 23640

attaaaccgg tgaaaatttc cttattattt aaactatctt tagctcctaa ctttcgacac 23700

agctcctgag tttgttccgt cctagcacag gtcagcccat aataaatgtt tgctccccac 23760

tcggtgaaca gccttattac gtcatagtta ttttctttta tggccatgat taatgccaca 23820

tcaagatgaa gaagttcccc cttaaagggg gttgagctta aaataacgta attacagtag 23880

tgacataagc taatgggctt gttttgccac cataagccac aatattttaa aatataatga 23940

tactcctcag gcacgctctg tttggccaca gcctttttgg ccagggtttg caaggagagc 24000

atgataactt cttgaaaaaa aaactcaaat taagttccta cttttttaaa atattagtat 24060

ggacagatct accatcatat gaaggaattc tttcatcgtt aaacactgaa gagataatac 24120

tttcatcgta tagagaatat catgtcaatc catatattga atgttatata tcattaaacc 24180

catcattaat atagtgttta tgtgctatgg acaggttttt tgaatgataa tcttttaaca 24240

tacgttttat aacttcggga tcagtttctt ttaaagataa agaatcattc atgttataac 24300

aatttaatga taacatgctg gcaatgaacg agttgtcttt ttgatgcgct agagtctttc 24360

cctcctcaaa ggcattggcg cctaagtcta tacaaaagaa tatgtttccg atattataga 24420

actgaataga atgaaacatg gcctgattga tatcagcccc taagacgacg caacagtaat 24480

aaatcgttaa atagttatag ttcttgcgac aggcccactt tagcatttca ttcatgtcta 24540

tgcgaatcct ctccttttcg tacacttcgt gaagttcaaa cacattattg taaaaaaggg 24600

cgcacataag ccgccaccga tgtagatgag catatctctg ataaaaatag caaatcgcct 24660

ccttaaggtt acattctatt gccatcgcgt accaatattt agtaaacatc tcgcttaata 24720

tatcggtttc taccattaat ccctccagtt gttcataaat cattcccttt acttcaaaac 24780

gatttatggt atctaaaatg ggattattag aaaatacctc atggcagaaa atgatgttac 24840

tgctagttag atcacgtttc aatgtgtaaa aaaatcgtaa aatttcctgg tcatttaact 24900

gttctttggc acctagctgc ctgcacaggt ctcgggtgtg ctccgtgttg acagaaagca 24960

aaccgtagtt gatgtttgca ccccactcgg tgaacaattc tattagatcg tgattgtttt 25020

cctccacagc tttcaccaag gccgcgttaa gatttgtgcc gttcttaaaa tacggcgtcc 25080

atattttctt ttgatgatac atgatagggc cattatgcca ccatagaccg cagcacttca 25140

aaaaatgagg atggcatttg gccggatact ggctggccag cacctttttg gtgagagtct 25200

gcagagagag gaccatattt cttttttttg aaaaaatcaa attaaaaaaa tcatgcttgt 25260

ttagcataca tgtaatattg ttataattac gttataatta cgttataatt acgttataac 25320

tatattataa caatggtata acaatggtat aacaatgtta taacaatgtt ataacgatgt 25380

atcattgatg tcatcattca actaggccaa catacttttt aatttatagt tttttaatag 25440

atgatatatt ttgctaggat ctgcttcttt taacgttaat agcgaggagt ctgcactata 25500

aatgtctaat gataaatgat gagatatcaa atagtaattc cgttgctctg ctagggcctt 25560

tgcctcttca aaggcgtcgg ctcccagatc tatacaaaag aacaagttat ccatattata 25620

aaatcgtacg caggcaagca tagctgaatt aatattagct cctaagagaa aacaataata 25680

tatggttaaa aaattgttat cttttgtgca ggccatccgc atcatttcat ccacgtccat 25740

gcggatcttt tccttttcat acaaattatg taggtcaaac agcttattaa aacaaagagc 25800

acagattaac caccacgtat ttagatactt aaaatgttgg taaacataag aaatggcctc 25860

cctaagatta tcctgcaatg ccactataaa acagtatatc gttaacatat caccatccga 25920

catattactt aatatgtcgg tgtcttctac taaccttttc aacttccaat atatggatga 25980

ccttatttcc cttataatga cataggctgg aaagggatta tcattaaaaa gtttaagaca 26040

taagataata ttactgctag tagtgccagg gtgtattaat ttaaagaaca tgtgcataat 26100

cttcttttta tccacgcggt acttggctcc taattcccag caaaattctc gaacaggcgg 26160

cgtattggcg caaattaacc catagttgat gtctgcgccc cattctgtaa acagttttat 26220

taactgatag ttgttttcct ttgtagccaa cattagtgcc gtattaaggt ccaagccgtc 26280

tgcaaagctt ggcagcttta tcagcatatg tttgcaatca agggaaattg gggccttata 26340

ccaccatagt ccgcagcgtt ctaagataac atggtactca atagatactt gctgtctggc 26400

tagtaccttt ttggcgaagg attgtaagga aggaaacatc ctgtttcttt tttttttaaa 26460

aatcaattat ctttgttcat aatcaagaaa aatccccata tttattgagt gataattttt 26520

taacatgcaa tttatttttt cagggtccgt aacgatcgac aacagagaaa taaccggatt 26580

gtaatgcttt aatgataagg catgggctat cagataattt tccttttgtt ctgccaaagc 26640

tttgccctcc tcaaaggcat cggcacccag gtctatacaa aagaacaggt ttccaagatt 26700

atagttttgt atggaaacaa gcatggcttg attgatgttg gctcccatga taaaacagta 26760

gtaaatggcc gaatagctat aatcttggat gcaggctatg tgcatcattt catcaatatc 26820

catgcggacc ctttctattt cgtacagctc gtgaaggtcg aacacgttgt tgtaaaaaag 26880

ggcgcacatg agccgccacc tatgtagacg cgggtatttc tggtaaaagt agcggatagc 26940

atctttgagg tcatagtcca ccgctatcgc gtaccagtat ttggttaaaa cagtgctaaa 27000

gctatcatca tggtccagca tgaaggttat ctccatgagc cctcttaact cccacatgat 27060

ttcccccctc agatccagat tatctataat ccttaaattg gggttattgg aaaacacctc 27120

gtggcaaaag ataatattgc tactggtttt atcgcgcgtt gtatcaaaga aaatttttaa 27180

aatatactct ctttctaaat attctttggc tcccagctct ttgcacagat cacgggtatt 27240

ttccgtgaga gcacaaatca ttccatagtt aatatctgca ccccattcag taaacagctt 27300

tatcaagtca tgattattct ccttcacggc tttcatcagt cctatgttta actcgatacc 27360

ttgactaaaa caggttgacc ttataaataa tttattgcgt cgaatatgaa gcataatggg 27420

gccattatgc caccacaggc cacaacactt caggacatga tattgatcta ccggtataca 27480

ctgcccggcc agtactttct tcgtgaggga ttgcagggaa ggcaacatgc ctttccatcc 27540

tttgacggaa atcaaattat ctactaataa ctatcagtgt ttatattaag tatttagata 27600

ttatcccggg ctggatacgt agtatcgcta ttcacatgta cttccaactc tagccggagc 27660

ctgcagggtc atttattttt aatattgatt cttttttgta tttaatcatt tagagaaggt 27720

catcatagga gccagatgtt ctctctccag aacttatgtc gaaaaacatt acctaaccgt 27780

aaacttcctg aattttttga cgaatatata ttacaactgc tgggattata ctgggaaaac 27840

catggaacta ttcaacgagc aggaaacaac tgtgtgctta tacagcaaca taccctcatt 27900

cccgtaaatg aagccctgag aacagcagca tctgaagaaa attatgagat cgtgagcctt 27960

ttattagcgt gggaggggaa cctttactat gctattatag gggctctaga gggcaaccgc 28020

cacgacttaa ttcgtaaata tgatgaccaa atcaaggacc atcatgaaat tctgccattc 28080

attgacgatc cagtcatatt tcacaaatgc catatcatgc ggcaatgctt ttttgattgt 28140

attttatatc aagctgtaaa atatagtaag tttcgcgttc ttctttactt taaacataga 28200

ttagaggatg atttgccctt cactcattta cttattgaaa aggcatgtaa agatcataat 28260

tatgaagtta ttaaatggat atatgaaaac ctacatatct acaatatgat agataccttt 28320

gaatgtgcta ttgcccataa ggatctacat ctatattgtt tggggtatag atttatatat 28380

aacagaatcg tacccgataa gtatcatcat ttagatattc gcatgctttc aagcctacaa 28440

ctcctacata aggtggcagc caaaggatac ttagatttta tcctagaaac cttaaagtat 28500

gatcataata aagataatat aaatattatt ctaacacaag ctgcaaccta taaccataga 28560

aaaattttaa tctatttcat tcctcaatca acccacgcac agatagaaca atgtttacta 28620

gtggcgataa aagcaaaatc ttccaggaaa accttgaact tactactgtc tcacctaaac 28680

ctttccatca acctcatcaa aaaaataagc cattatgttg ccacttacaa ttcaacaaat 28740

ataataggca ttctgagtat gcggcggaaa aagaagatat atttagatat catattgaca 28800

aaatttgtaa aaaaagctat ttttaataag tttgtcgttc gatgtatgga tacattttct 28860

ataaacccgg aaagaatcct taaaatagcc gcgcgaataa ataggatgat gttagtgaaa 28920

aaaatatctg aacatgtttg gaaaaatcat gcggttagac ttaaatacct taaacatgcg 28980

gtacacacga tgaagcataa agatgggaaa aatagactca tgaactttat ctatgatcgc 29040

tgttattacc atatgcaagg ggaagaaatc tttagcctcg caagatttta tgcaatccat 29100

catgcaccaa agttgtttga cgttttttat gattgttgta tcctagatac gatacgattc 29160

aaaagccttc ttttagattg ttcacatatc ataggtaaaa acgctcatga tgctaccaat 29220

atcaacatcg tgaacaagta tatcggcaac ctgtttgtta tgggagttct tagcaaaaaa 29280

gaaatcttac aggactatcc atccatttat tctaaacaat acatgcctta gtttattttt 29340

tttgcggccg aaacattatt cttaccctag aaaacgctta tagtcatctt aaatcatagg 29400

taaggaagat catcatattt tttgaaacgt aattttttaa cgcatgatct atgatttcag 29460

ggtccgtgct tttaggcaac ggggtggtgg ccggactata aatctttagg gataaaatgt 29520

tctttataag ctcataccct tcccctaaag ctgtagtacc ctcttcgaaa acatcagccc 29580

ccagatctat acaaaagaac atgttttcta tattatagta ctgtattgag ctaagcatgg 29640

cttgattgat gttggcgccc aggacatagc agtagtacat ggttgaaagg ttgtggtctt 29700

tgatgcaggc gatccgcatc atctcttcta tgtccatatg gatcttgtcc ttttcatacg 29760

cctcatgaag gtcaaacaca ttattaaaac aaagagcaca tgttaaccgc cacgtattca 29820

ggtgtgtata tttttggtaa aaatactgta tggcctcttt caggttatag cgtatggcta 29880

tagcgtacca gtatttgagt agtaatgtac tgagcgaaaa ctcattattt agcagatcgg 29940

tttttactat taactccctt aactcccaga aaatttctat cctcattttt atattattta 30000

ctttttgtaa tatcggattg ttggaaaaca cctcatggca taaaataatg ttactactag 30060

ttttatgaaa ctttagatct ataaaaattt gtaaaatttc ttcttcattc aaggtttcct 30120

tggcacctag ctctcgacag aggtcccagg tgtgctccgt gttgacagat accagcccgt 30180

agttgatgtc cgccccccac tctgcaaaca gttttataag gttgtagttg ttttccctta 30240

cagccttcac taacgccgta tttaggttta agccctcttt aatacctgct gattttatga 30300

gccttaggtt atgatcaaac gtgatcggag catcatgcca ccataggtca taacacttta 30360

aaagataatg ttggttcgtg ggcacgcatt gtccagccaa cacctttttg gtcagagatt 30420

gcagggaagg caacatgtct cttcatcttt taaaaaaaaa tcaaattaat tagccgaata 30480

aatttttctt tcgagggctt tttaaaagag ctctttaaga gctctttaag agctttttaa 30540

gagattaaaa aattattctt gctggcattc tgccaagtat gcggcattcc tatcatctat 30600

agtatattat gagaatattc ccaaatgatg gataagtttt ttgatttata atcttttaat 30660

aaactgctta tttcttcggg gtcctttaag tttagtggca aggaagcatc tgagctgtaa 30720

atatccaaag ccaaactatg gctcagaaaa ttataacctt tttgttccgc tatggcacga 30780

ccctcttcaa aggcattacc acccaaatct atacagaaaa atatattacc gatgttataa 30840

tattgtactg aagtaagcat agcttggttg atgttgcccc ccagcgcgta acagtaatat 30900

attgttaatg gattgttatc cttggtagaa gccagacata tcatgtcatg gacgtctatt 30960

tggatgtttt ccttgtggta catctcatga agctcatata ttttgttata atacaggaga 31020

cattttaatc gccattcatt aagatccgta tatttctcat ctagaaaaca aatggcgtcc 31080

ttacaatcgt attgtactgc tttggcgtac caatacttca ctagtaaacc atttaactcg 31140

tccgtttctt ttatttctat gagcccccat agtcttttat aaattaagcc ccttaattgt 31200

ataacaaatt tgttttctaa aataggatta ttcataaaaa tttcatggca caaaataata 31260

ctgccgctgg ttttattgtg cattatcctg gtaaaaatac ggaaaatatc gttgtcctct 31320

agagtttctt tggcgcctag ctgtctacac aactctcgga tgtgcttcgt attgatagaa 31380

agcaaaccat agttgatatt tgcgccccac tctgtaaaga gctttatcag actatagttg 31440

ttttccttaa cagctattat taatgccaca cgaaggtcta tatcttctcc taaaaatcct 31500

gattttattt gtattcggcc acgatccata caaagcttga gaggagcatc atgccaccat 31560

aggccacaat atttcaaaat gcagtgttca tctattgaca aacactggct ggctatcgtc 31620

tttttgacga gggtctgcag agagagcggc aacgacatgt ttctttttca ccaaaaaaaa 31680

atcaaatgtt ctcgtcttta aaggttaatt catgttctta aaatgttcat ttcatgatag 31740

tgattaataa tatggtttaa taacgctaga aggcttgttt ataagacagt cataagcagt 31800

ctataagaca gtctataagc agtctataag acagtctatg acttagtcta taactataat 31860

ttctggatgg gctgtaagat actcttcggc tcgtttcaga ttttttgaag tatatgtctt 31920

tagcatatca tatatttcct ggggttcggt tacatctaat accaaggtca catcacggct 31980

gaaaagctgc tttactaaga aaatgttgct caagttatac atataagctt tgtgcgcaat 32040

gagttgtgcc ctatcaaaat cggcagcccc caaatcaata cagaaaaaca tgtttaaagt 32100

attattgtta tagatagaaa gattcatgcc ataatcgaga ctagccccca acctatgaca 32160

gtaataaatg gccgcgtaat ttttttcccg caagcaagca aatttcatca tcagattagg 32220

gctgatgcaa atctcttttt cacgacacaa ctcgtgtatg tcaaaaatgt tattaaaata 32280

aaggctacaa gctacccgcc aatagaggtg atttttatgc cttttataga aatagtgaat 32340

agcctttgta aaattatgtc gtaatgccag ggcaaaccaa aactttgtta ataggtggtg 32400

cgccgtatcc cccgtcaacg gaatgtttga acaggtgtac gtaactgtgt ctaaagtggt 32460

tctagttacg gtttccaaga gtggattatg acaaaacatg tcataaccca gcagaactcc 32520

tgcacaggat tttagcctgg ccacttcttt taaaatttcc agaagacggg gttcggatac 32580

aggcgttaag cctcccagtt ccgcacacag ccgctttaga tacacggcag gaacacgtat 32640

aagcccatat tcaggatttg cgccccaatc cacaaataaa cgtataagtt caagattatc 32700

gctcttcacg gcctttacta gcgccgcttc gagacaaaga tcatcctcag aaaaacactg 32760

taaatgttta tacgaaaaaa cttgcttaca attgttacat aggtgaatag gacctaaatc 32820

ccaccacaaa ccaaaacgct gcaacgtata atcatagtca cttgaaagat aattgcatgc 32880

cacaactttt ttggccaacg tttgtaaaga caacatacta agtttaaaac atcttaaatc 32940

taagctagct aactttcaag aaaaccctct atccctaaga atatatctta taactagact 33000

tatagcagta aaaatcaact ttggttattc tttttaatat aaaacgtcta attacttgca 33060

aaggactata aagcccattt tcctcagcta gaatttttat tttttaatga agtaggggga 33120

tatgttttcc cttcaagacc tttgccgaaa gcatcttttt attcttcccg atgtttttgg 33180

cgagcatgta ctacaacgat taggactgta ttggagatgt cacggctccc ttcaacgcat 33240

aggagacgac cacatactca tacgacggga tctcatcctt tccaccaacg aggccttaag 33300

aatggcggga gaggaaggaa acaatgaagt agtaaagctc ttgttactgt ggaagggaaa 33360

tcttcattac gccgtcatag gagccttgca gggtgatcaa tatgacctga tccataagta 33420

tgaaaaccaa atcggcgact ttcattttat cttaccattg attcaagacg cgaatacgtt 33480

tgaaaaatgc cacgctttag aacgtttttg tggtgtttca tgtctgctaa aacatgctac 33540

aaaatacaac atgctcccta ttctccaaaa ataccaagaa gagctgtcta tgagagcgta 33600

tcttcacgaa accctatttg aactagcatg cctatggcag aggtatgatg tccttaaatg 33660

gatagagcaa accatacatg tttacgacct aaagattatg tttaatattg ccatctccaa 33720

gagggatctg actatgtact ccttaggata tattttcctt tttgatagag ggaacaccga 33780

agctacgttg ctaacgcaac atctcaagaa gacagcggcc aaagggctcc tccactttgt 33840

gctagaaacg ttaaaatacg gcggcaacat agataccgtc ctgacccaag ccgtaaagta 33900

caatcataga aaacttttag attattttct gcgtcaacta cctcgtaaac atattgaaaa 33960

acttttgttg ctggccgtgc aggaaaaggc ttctaaaaaa acattgaact tactgttgtc 34020

acatttaaac tactccgtga aacgcatcaa aaaactaccg cgctatgtga tagagtacga 34080

gtccaccttg gtgataaaga ttttattaaa aaaaagagtg aacctgatag atgccatgtt 34140

ggaaaagatg gtaagatatt tttctgcgac gaaagtgagg acgatcatgg atgagctttc 34200

gattagtccg gaaagagtca ttaagatggc tatacagaaa atgagaacgg atatcgtaat 34260

ccatacttct tatgtttggg aggatgatct agaacgtctt actcgtctta aaaatatggt 34320

atacaccata aagtacgaac atgggaaaaa aatgttaatt aaagtcatgc acggcatata 34380

caaaaactta ttatacggcg aaagggaaaa agtcatgttt tatttagcca agctctatgt 34440

tgctcaaaac gcggccaccc aattcagaga catttgtaag gactgttaca aactggatgt 34500

ggcacggttt aaaccgcggt ttaagcaact aatattagac tgtttagaaa ttattactaa 34560

aaaatcttgc tatagtatcc tggaaatctt agaaaaacat attatttccc tgtttactat 34620

gaaagttatg actgaagaag aaaaaaacct atgtttagaa atattatata aagtaattca 34680

ttataaaaca atacaatgtt aaaattcaat agatatccat cattaatatt gattatattt 34740

tcgaatatta tcttctatgg tgcaagataa tcatctagcg cgtgaaacat gtcctcttct 34800

cttcaggaac tttgtcgaaa aaagctgcct gactgcatac ttccagagtt ttttgacgac 34860

tatgtattgc aactgttagg actgcactgg caagatcatg gttcccttca gcgtatcgag 34920

aagaaccaga tacttgttca acaggaaccc atccatatca atgaagcact caaagtagca 34980

gcatcggaag ggaactatga aatcgtagag ctgttgttgt catgggaggc agatccccgc 35040

tacgccgtcg taggagccct agaaagcaaa tactatgacc tggtttacaa atactatgac 35100

caagttaaag actgccatga tatcttgccg ctgattcaaa atccggaaac attcgaaaga 35160

tgtcatgagt taaacagcac ctgttcactg aaatgcttat tcaagcatgc tgtgataaat 35220

gacatgctgc cgattcttca aaaatataca gactatctgg ataggtggga gtattgcagc 35280

cagatgctgt tcgaactggc atgtagtaaa aaaaaatatg agatggttgt gtggatagag 35340

ggagttctag gcgtcggcaa agttacatct cttttcacca ttgcgattag caacagagac 35400

ctacagctgt attctctggg ctactcaatt atccttgaga atttgtactc ctgtggacag 35460

gaccccaagt ttttactaaa tcatttcctg cgagacgttt caataaaagg gcttctaccc 35520

tttgtaatca aaaccataga atatggtgga agcaaggaga tagccataac tctggctaaa 35580

aaatatcagc ataaacatat tttgaaatac ttcgaaacct gggaaagcta ggttcagtat 35640

ggtgtactca ctattgtagt gaatcgtatc ctgtaaattt tgtaaaaaag cttaaacttt 35700

tgaccacatc atattgtttt agaaatctca aaccagtgaa caacagtctt atcatacatt 35760

aaaattccag taaaatttat attttttttg gtaaacaaat gttttctctt caagacatct 35820

gtcggaaaca tctttttcaa cttcctgacg cttttgatga atatatatta caagcgctag 35880

gactatactg ggaaaaacac ggatctcttc aacgaataag aaaggacgct gtgtttgtac 35940

agcgaaacat cgtcctttct accaatgagg ccctgagaat cgcagcctca gagggaaacg 36000

aaagggtaat aaaacttctg ttatcatggg agggaaattt tcattatgtg atcataggag 36060

ctctagaggg tgaccaatat gacctaattc ataagtatga tagtcaaatt aaagactacc 36120

acatgatttt atcattgatc caaaatgcaa atacctttga aaagtgtcat cagttatcca 36180

atagtaatat gtggtgtctt atacagaatg ctataaaata taatatgctc cctattctcc 36240

aaaaacacag aaatattctg acacatgagg gagagaatca ggaattgttt gagatggcat 36300

gtgaggaaca gaaatatgac atagttttat ggataggaca aaccctaatg ttaaatgagc 36360

cggagtttat ttttgatatc gccttcgaac ggatagattt ttctttatta acaatgggtt 36420

atagccttct ttttgataac aagatgagta gtatagacat tcatgatgaa gaagatctta 36480

cttcattacc aacagaacac ctcgaaaaag cagccactaa gggatgtttc ttctttatgc 36540

tagaaacttt aaaacatggt ggaaatgtaa atatggcagt cttatctaaa gctgttgagt 36600

ataatcatag aaaaatttta gaccatttta ttcggcggca aaaatgttta tcacgtgaag 36660

agattgaaaa cctattatta accgccataa ccaattgtgc atccataaaa acgttaaact 36720

tactcttgtc ttacctaaac tattccgtaa aaaatatcat tggaaaaata gtacaacatg 36780

tcataaaaga tggtgattat accatcatat tacttttaaa aaaaaagaaa ataaacctag 36840

tggaacctgt tttaacaggt tttatagatt attactatag ctattgtttt ataaaacatt 36900

ttatccaaga gtttgctatt cgtccggaaa aactgattaa aatggccgcg cgaaaaggta 36960

aactaaatat gattatcgaa ttccttaacg aaaaatatgt tcataaagat gatcttggaa 37020

ctatatttaa atatctcaaa accctagtat gtaccatgaa acataaaaaa ggaaaagaga 37080

cattaattgt tcttattcat aaaatatatc aagatattca tctggagact aaagaaaaat 37140

ttaaattatt aagattttat gtcatgcatg atgcaactat ccaatttcta tctatgtgca 37200

aagactgttt taatttagcc ggttttaaac catttgtttt agaatgtttg gatattgcta 37260

ttaaaaaaaa ttaccctgat atgatacaat atatagaaat tctatcgaaa tctgagtaaa 37320

atttattttt ttgatcagag taagaaaatg ttctccctcc aggagatctg tcgaaagaac 37380

atctactttc tacctgactg gctcggtgag catgtgattc agcgactagg tctgtactgg 37440

gaaaaacatg gttctcttca gcgaatcgga gacaactatg tacttataca acaggacctc 37500

atcatcccca tcaatgaagc cctaagaatg gcaggggagg aggggaatga tgaggtggta 37560

caactcctat tactatggga gggaaacatt cattatgcca tcataggagc tttggagagt 37620

gaccattata gcctaatacg taagctctat gaccaaatcg aagactgtca cgacatcctt 37680

cccttgattc aagacccaaa actctttgaa aaatgccatg aattagataa atcttgtaac 37740

attttatgtc tcgtattaca cgccgtaaaa aacgatatgc tttgcattct tcaagagtat 37800

aaaatgcatc taagtggaga ggatattcaa gtggtgtttg aaacagcatg ccgttcacaa 37860

aaaaacgata ttgtgtcatg gatgggacaa aatattgcaa tatacaaccc cgaagttatt 37920

tttgatattg cctttgataa gatgaatgtg tccttattat ctatagggta tacgcttctt 37980

ttcaatcatc atataaataa tacgaacgaa aatattaatt ctttattgac acaacatctt 38040

gaatgggctg ccggcatggg ccttcttcat tttatgctgg aaactttaaa gtatggcggg 38100

gatgtaacga taatagtctt gtctgaggcc gtaaaatatg accacagaaa gattttagat 38160

tattttctcc gtcgaaaaaa cttgtaccaa gaagatcttg aagaactatt attgttggcg 38220

atacgtgcag attgttctaa aaagacctta aacttgttat tatcttactt aaactattcc 38280

ataaacaata tccgtaaaaa aatattacaa tgtgtaaaag aatatgaaac gaccgttatt 38340

ataaaaattt tacggaaaag aaagataaat ctgatagagc ccattttggc agactttata 38400

ggatatcata gctataccta tatggtagat tttatgcgtg agttttccat ccatccggaa 38460

aaaatgatca aaatggctgc acgagaatcg agggaggact tgatcataaa attttccaaa 38520

aaagtttgca aagagcctaa agatagactt cactatctca aaagcttagt gtatactatg 38580

cgacataaag aaggcaaaca actgttaatt tatacaatcc ataacttata caaagcttgt 38640

catctagaga gtaaagaaat gtttaatttg gcacgatttt atgcacggca taatgcagtg 38700

atccagttca aatcgatttg ccacgatctc tccaagctca atattaatat caaaaacttg 38760

ttgttagaat gtttaggtat tgctattaaa aaaaattact ttcaacttat caaaacaata 38820

gaaacggata tgcgttatga gtaacatttt tagatgaggg aagattctac caaactaact 38880

aagacctttc gctagaatgt atcttattgt taatatagat gagatatgtc attgtgaaaa 38940

aatagattag gtaggttgtg aaaaacagat taaacttaaa attatgtgta ttatgtaaaa 39000

ttttagaaat aaaaatttat ttttttttat tgagggtacg gaaaatgttc tccctacagg 39060

acctctgtcg gaagaacatt ttcttccttc caaatgattt tagcaagcat accctacaat 39120

ggctgggatt atattggaaa gagcatggat ccgtccatcg agcagaaaaa gacagcataa 39180

tgatacagaa tgaattggtt ctttctatca atgatgcttt acagcttgca ggagaggagg 39240

gggacacaga tgtagtacag ctcttgttat tatgggaggg aaatctgcat tatgccatca 39300

taggagcctt gaagactgaa aaatataacc taatatgtga gtatcatagc caaattcagg 39360

actggcatat tctcctaccc atgattcaag atccagaaac attcgaaaaa tgtcatgatt 39420

taagccttgg atgtgacttt atttgccttc tccaacatgc tgtaaaatac aacatgcttt 39480

ctattcttgt caaatataag gaggatctac taaatgcaag gattaggcat cgtatccaat 39540

ccctgtttgt tttggcatgc gaaaatcgga gaattgaaat tattgattgg ataggccaaa 39600

atctgccaat tcctgaacct gatgccattt ttagcattgc tgttgctaca agagatttag 39660

aactgttttc cttagggtac aagattattt ttgattacat gcaaagacag ggaatcattc 39720

aattaaccaa tggagttcgc atggttgtgc taaatcgtca cattagcatg gcaatagata 39780

atggtctttt accttttgtt ctggaaactt taaaacatgg tgggaatata catagagcct 39840

tatcttatgc agtaacacac aatagaagaa aaattctgga ttatcttatt cgccagaaaa 39900

atatagcccc taatacaatt gaaagacttt tatatctggc cgtgaaaaat caatcttcca 39960

ggaaaacttt gaacttgttg ctatcttaca taaattacaa ggtgaaaaat gttaaaaagc 40020

tggtagagca tgtagtaaat gagaaatcca ctcttgtgtt aaaaatttta ttagaaaaaa 40080

aggaaaatct agtggatgct gttttaacaa gacttgtaaa acattctaca tatttccagg 40140

tgagagaatt tatccaggag ttttccatca gcccagaaaa attcattaaa atagctgtgc 40200

gggaaaagaa aaatgtgtta atcgaggcta tttctgaaga tatttgggaa aatcccacag 40260

aaagaattac ttatctcaaa cagatagtgc acaccataaa atatgaaagt ggaaggcgat 40320

ttttggtaga catcattcac agcatttacc aaagttactc actaaaacac gaagatattc 40380

ttaaactggc aacattttat gtcaaacaca atgcaatcac ccattttaaa gacctctgca 40440

aatatctttg gctgaacaga ggaacagaaa gtaagaaact gtttttagag tgtttagaaa 40500

ttgctgatga gaaggagttt cctgatatta aaagtattgt gagtgaatat attaactact 40560

tgtttactgc aggagctatt accaaggaag aaatcatgca agcctatgat gctttagagt 40620

agccatgtat taacattctg aaagtagaat aaaatatact atatactaaa aaccaaatta 40680

gccattttta actatcttct tcttaaaaac tctggataaa aatttatttt tttttaattt 40740

gggtagggaa aatgttctcc cttcaggacc tctgtcggaa gaacaccttc ttccttccaa 40800

gtgattttag caagcatacc ctgcatttgc tggggttata ctggaagggg catggatcta 40860

tccaaaggat aaagaatgat ggtgtgctta tagagcatga tcttactctt tccatcaatg 40920

aagccttaat tcttgcagga gaagagggaa acaatgaagt agtaaagctc ttgttactat 40980

gggaaggaaa tcttcattat gccatcatag gagctttgag gactgagaac tataacctag 41040

tatgtgagta ccatagtcaa attcaggact ggcatgttct cctccctttg attcaagatc 41100

cagaaacatt cgaaaaatgt catgatttaa gccttgaatg tgatctttca tgccttctcc 41160

aacatgctgt aaaatataac atgctttcga ttcttgttaa atataaagag gatctactaa 41220

atgtactatt taggcaacaa attcaaggac tatttatttt agcatgtgaa aatcggaagc 41280

ttgagattct tacgtggatg ggtcaaaatc tgccaattcc tgatcctgag cctattttta 41340

gcattgctgt tgtcacaaaa gatttagaaa tgttttcctt agggtacaag attgtttttg 41400

aatacatgga aaaccaagga cttcatttaa cccaggtagt tcgtatggtt atgctaaatc 41460

atcactttgg catggtaata aataaaggac ttttaccctt tgtgctggaa attttaaatt 41520

atggtgggaa tgtaaataga gccttatctt atgctgtcac acaaaataaa agaaagattt 41580

tagaccatgt tgttcgccaa aagaatatac cccataaaac cattgaaaga atgttgcatc 41640

tggctgtaaa aaagcatgct cccaggaaaa ctctgaactt gttactatct tacataaatt 41700

acaaggtgaa aaatgttaaa aagttgttag aacatgtagt gaaatacaac tctactcttg 41760

tgataagact cttgttagaa aaaaagaaaa acctgctgga tgctactttg acaagatatg 41820

tcaaagattc tacatacttt caggtgaaag aatttatgca agacttctcc atcagcccag 41880

aaaaattcat taaaatagct gtgcgggaaa agagaaatgt gttgatcaag ggtatttctg 41940

aagatatttg ggaaaatccc gcggaaagaa tcaggaatct taagcagata gtgtgtacca 42000

taaaatatga aagtggaaga caattcctga taaatatcat tcacaccatt taccagagtt 42060

attctttgaa acctgaagaa attcttaaat tggcaacatt ttatgtcaaa cacaatgcaa 42120

ccacccattt taaagatctc tgcaaatatc tttggctgaa cagaagaaca gaaagtaaga 42180

aactgttttt agagtgcttg gaaattgctg ataagaagga gtttcctgat attaaaagta 42240

ttgtgagtga atacattaac tatttgttta ctgcaggagc tattaccaag gaagaaatca 42300

tgcaagccta tgctttggag tatgccatgt attaaatttc tgaatcagta agcaatagat 42360

agattttaga atatgctgta ttaagttagt ttctgaataa gtaattaata gatagatttt 42420

agtttatgta aaaatgttaa catttgttca taagttttag ataccatttt agagttactt 42480

ttttagatat tactatttta gccattatta tcttaaataa tcactatttt agataggtcc 42540

ccgtattaaa aaccaaatta accattatct atgtttttaa taatactttt taaaaaccct 42600

ccataaaaat ttattttttt ttcataaaag tagagaaaat gttctcccta caggatctct 42660

gtcggaagaa cctttttctt ccacttgagc ccttaggcaa gcatgtggtt caacggctgg 42720

gattatactg ggaaggccat ggttcagtta aacgagtggg tgattgcttt atatgtgtag 42780

accagatttg gatgctatca atccataagg ctatacaaat tgcagcctcg gaaggaaatg 42840

agaacattgt caagcttttc ttactatgga aggggagtct acaatatgcc atcataggag 42900

ccttagaggg caggcaatat gatctgattc aaaaatatta caaccaaatt ggggactgcc 42960

atcagattct accactgatt caagatccag aaatttacga aagatgtcat gaattaaatg 43020

ttacatgtac ctttcaatgc ttatttcaac atgctataag agataacatg ctgcccattt 43080

tccaaaaata tggagaagat ctgaatggaa acaggagaat ggttcaactt ctgtatgaga 43140

tggcatgccg attacaaaat tatgatatca tcaaatggat aggatctaac ctgcatgttt 43200

ataacttgga agccattttt agcattgctt ttgttagaaa ggatttaact ttgtattctt 43260

taggctacat gcttcttctg ggtagaatga gtactgaaga tagaaacttt atctcaatca 43320

taacacgcca tcttgaatac gcatcaaaaa agggactttt tgactttgta ctagaatctt 43380

tgaaatatgg aggtcaagtg gatacagtgt tgtttcaggc tgtaaaatac aaccatagga 43440

aaattttggc ccattttatt catgaaattc cccgtgaaac ggttgaaaag ctgatactcc 43500

atgctgtgga gtcacgggcc tccagaaaaa cattcaacct gcttttatct tccataaact 43560

actgtgtgaa cccttttgtc aaaaaactac tgcacgctgt ggtggaacac aagtacatgc 43620

ttatcataaa gcttttgctc gagcggccca aaaagaagat aaacctggta gatgctgctc 43680

tattcaaact tgtaaaatac tctacttata cagaaatagt aaaatacatg ggtgagtttt 43740

ctgtggaccc aaaaagggtg gtcaaaatgg cagcacgact catgagagtg gacctgatta 43800

aaaagatttc taatgatgca tgggaagata aactagagag aatcaagcac cttaaacaga 43860

tggtaaatac catgaaccac agaaatggaa aaaatctatt gatgtacaat attcacaata 43920

ttactggata tacctatctg aacaccaaag aagcatttaa cttaacaaga ttttatgctg 43980

tccacaatgc aacatgtttg tttaaagaaa tgtgtaaaag ctgttttgta catgataaaa 44040

tacagctcag agaattgctt gaagattgtt tacatattgc taataggcat gattatatcc 44100

agattgcaga aaccgcagat gaatgtatca aatatataga tcttattaca tttaagtaaa 44160

ccatgtatat atcaagtaaa tccagattaa atcaggctaa ttgtaaatag ttgtagatac 44220

catataatga atgttttatt aggatagtag ttcagttaag atagtagttt agttaagata 44280

gtagtttagt taagatagta gttatgttaa gatagtagtt ctgttaagat aatagtttag 44340

ttaaaactag ttcatgttaa gttaatagtt ttgttaagac aatagttcat ttaagtcaat 44400

agttcagtta agtcaatagt tttgttaagt caatagttta gttaagtcaa tagtttagtt 44460

aagtcaatag tttagttaag tcaatagtta tattaagaca ttagttctgc taatacatta 44520

gttttgttaa gataataaaa atttattttt ttttcatcag ggtagagaaa atgttctccc 44580

tacaggagct ctgccggaag aacatttaca ttcttcctta ccccttggct aagcatgtac 44640

ttcaacaact agggctgtac tggaagggac atggatctct tcaacgaatc ggagatgacc 44700

atgtactctt acagcaggac ctgatctttt ccatcaacga ggccttaaga atggcaggag 44760

aggaaggaaa caatgaagta gtaaagctct tgttactatg ggagggaaac cttcattatg 44820

ccatcatagg agctttagag ggcgaccgat atgaccttat ccataaatat tatgatcaaa 44880

ttggggactg ccacaagatt cttcctttaa tccaagaccc gcaaatcttt gaaaaatgcc 44940

atgaattgag taactcctgt aatattcgat gccttttaga acatgcagta aaacacgaca 45000

tgctttctat tcttcaaaaa cacaaggagc aaataagatt acacatggca ttaacccaaa 45060

tactatttga attggcgtgt catgaacgta aaaatgacat cattagatgg atcggttatt 45120

ccctgcacat acaccatcta gagactattt ttgatgttgc attcgcccat aaaaatttat 45180

ccttatacgt tttagggtat gaacttctca tgcacaaagt aaatacagag gctgcatata 45240

tagaattacc caatttgcta tcatatcacc ttcgaactgc ggcggcagga ggtcttctta 45300

actttatgtt agaaacaata aagcatggtg gatatctgga taaaacggtt ttatccgcgg 45360

ctatcaggta caagcatagg aaaattgtgg ctcattttat tcatcaggtt ccccgtaaaa 45420

ccgttaaaaa actgttactc tatgctgtgc aggctcgggc ccccaaaaaa acactgaacc 45480

tacttttatc ttccttaaac tactccgtgc acaccatcac caaacaactc gtacacaatg 45540

tcgtcatcta cagttccacg cttatcgtaa agcttttact catgcggcga aaaaacaagt 45600

taaacctagt agatgccgtt ttagccagac ttgtaaaata ttccacctat acagacattg 45660

tacaattcat gggtgagttt tctgtgagcc cagaaagggt gatcaaaatg gctgcacggg 45720

aatccaggac ctttctgatt gaaatgatct ccaaagctgc ttggggaaat cacccacaga 45780

cgttgattca tcatctcaaa caactaacca ataccatgaa gcctcaatct ggaaaagacc 45840

acatcatata taccatccac tatatttatc taaactctaa tatgctggta gcggaggagg 45900

aaaaaaatat ttttaaatta gcaaaatttt atgcgaatca taatgcggta aacaggttta 45960

aacaaatttg tgaagactat tatatattag atgcacgatt taaaacactt attttagaat 46020

gttttgaaat tgccgtccag aaaaactatc ctagaattgc aaatattgtg gatgactata 46080

ttcgattcct tttttacagg ggaaatataa ccgaggaaga aattcgtgaa gcctattctt 46140

taaaagatgc tgaggtttat gtagatttaa aatggttaca acaaggagaa atggtttaaa 46200

ccaaatccgg tttaaactaa atccaattta aactacattt ggtttatcat tagtcattga 46260

aaccatcgaa aaaaaagcta tttgtttatc cccataaact catctttttt ttgtctcaaa 46320

gtttgacact aaaattcagt gttttatagt gtttataatt aagtgttttg catgcattgc 46380

agaaattttc atctttttta attggttcaa taccacatgt catacaatat gttgtttgat 46440

tatcaagatt aactttatga aaggaaagta agtgagccgc aaatttaaaa gtaaaatatc 46500

tttcatttaa aatgatctta tgaatgtatt ttcgataagg aggaatgaaa gcatttgcca 46560

aaataaatcg cataaaaggc ttggaaaaac ccatatcttc taatcttttg tgggtataaa 46620

ccctattttg gtgttttaca aaaacttcat tgttataata gtcgttatag ctatcaatca 46680

tttttttaag tcctataatg cccaaggttg cacgcataaa gccacagttt ctgctccaaa 46740

aagcatgcac ctgtaaaggg tgcttttcat ataaccaatt acaaaatttc attccgcaac 46800

agtagcatgt tatttcagtg ggggatgtat agaataatcc ggcattcgaa aatttttcat 46860

aattttttat gtcatggatt gcgaagcttt gatttcgtgc atctatggag ctatagccta 46920

catatttagg ttttacttca aataatcgca aagagatgta tggatctatc gtatttattt 46980

taggaaacat ttcataattt taaattctta tatataatat aaaaaaaatt acaaacattt 47040

gtaatgatca tcctcaattg aaggctgagt tgtaggcttt atttttctaa ttatacgaag 47100

aaggtaggtt ctcataaagc cttcaagatg actattgatg tttccaatac attttctcaa 47160

tgagttcata aacccagaca ttttgctaat ggcttggcaa agtgccaaca agttgtccac 47220

aaagtactgg tagattgcca ctagctatag ctagctatag tgagccaacc tctctgtatg 47280

tattttatat atttcatttt ttaatagatt taatattttt ataaaaaaat atttagtttt 47340

ttatacaaga atgtcgacaa aaaaaaagcc cacaattacc aagcaagagc tttactcctt 47400

agtagcggca gatacccagt taaataaagc attgattgaa agaatcttta caagtcagca 47460

aaaaataata caaaatgctt taaagcacaa tcaagaagtt attataccac ccggaatcaa 47520

gttcaccgtc gttacggtga aagctaaacc tgctcgccag ggccataatc ccgccacagg 47580

agagcctatt caaattaaag ctaaacctga acataaagcc gtaaagatac gagcattgaa 47640

acctgtccat gatatgttaa actaaactat aaagtcatat tcttctttat cgttattatc 47700

ttcaatatat ttttgccaat cgaaatcgaa taaattcaga tcctggacat ttaaatactt 47760

atcatcgtac attttaatat aatttaaaca tgagttgttg tcaaaaactt ttagcgtttt 47820

tgttaaaatt atcatatgaa taatttcctt attaagagtt gccggaataa tacaaaacct 47880

atttttaggt acatcatcca tgataatagt aaaattagta aaaattgttt cttgtttttc 47940

ttttgtttca aataaacgtt gtaaggttaa aggtttctcg ttcaatggtt tctttgaaga 48000

taaaaagaat gtataatctg gtttaaaggt atttttggtt tcaatcgtga ttccatctgc 48060

ttgagcatat actaaaccag accaaatata acggtccact attacaatat aatttagctt 48120

aagtagcact gcaatttctg cgataaattc actacgatgt tttgtaaata atttatgtaa 48180

ttgttccgat gacatttcta tggttttatt taacacctgc aatataagat caccggtggt 48240

cgtgtctgga ttaggaaaat gtatacatat agcattataa tccatgcatt ccaatgtttc 48300

ttttaatttc attgcctgtg tgctttttcc cacaccattg attccctcga tggcaatgag 48360

tattccacgc atgattaata aaaggaaaaa aagaattcag tttttaacat ttcttacaaa 48420

tcttttttta tacaacattg tacaacactg cattagcggt atatgatgtt atagcttcat 48480

taaatatttg cttttatata atctttacca acctatattt ggtagatcac tgcagatggt 48540

cataaatagg ccataactaa gataaaaatt atttcagacg ctactacggt agtattatta 48600

aaatcatgtg tggcaatgta tgacgtctta atagataaaa catttaagga aaacaaattt 48660

gaataaaaaa aataattgtt atgatggcgt tgttacacaa agaaaagctt atagagtgca 48720

tctatcatga gctagaaaat ggcgggacaa tattgcttct aacaaaaaat attgttgtgt 48780

cagaaatttc atacattggc aatacttata aatattttac ctttaatgac aatcatgatc 48840

tgataagcaa agaagatctt aaaggagcaa catccaaaaa cattgctaaa atgatttata 48900

attggattat aaaaaatcct caaaataata agatttggag tggtgagccg cgtactcaaa 48960

tttattttga aaatgattta tatcatacaa attacaatca taaatgtata aaagattttt 49020

ggaatgtttc aacttcagtc ggtcctcata tctttaatga tcgtagcatt tggtgtacta 49080

aatgcacatc cttttaccca tttaccaaca ttatgtcgcc caatatattc caataaatta 49140

gatatctttg ctattaaaat agttaaaaac cttataggat aattaggtac tttattacga 49200

taaattatga tattttataa ttagttactt tattataatt aatctcttta ttaatgaatt 49260

atcataagat aactaattat ttttttccat atatcagata ataaatctga tatgggctaa 49320

aagtatgttt caaactattt acaatagaat ttctgttaag aaaacataca taatttgaat 49380

aaaatttttt taaatatcac cgaaacaatc aacatggtgt taatagagtt tttaacaggt 49440

ttcttctatt tatatggaaa gagactgttt tccattagta aagtcatgga catgatatgt 49500

ctagactatt ataccattat tcctgctcct ctggcgatga tgttagcggc aagactaaaa 49560

aactatgacc tcatgaaacg actgcacgaa tgggaaatct ctattgacta cgctctactt 49620

gtagtagatg atgtgccgtc tattgactat tgcttaagtc ttggcgctag atccccgact 49680

agagcacaaa aaagagaact gctgagggac aacacgttta atcccgtgta taagtatctt 49740

atgaactgtt ccggcttccc aacaaagaga gaaaaaaaca ttccttgtga tgttcaatgc 49800

gaaagactgc aaaaaaacat tataaaagaa ctggtattta actgctctgt actgcttgaa 49860

atggtactgc acacagaaag agaatatgca tacgccctac actgtgctgc aaaacataac 49920

caattgccca tcctcatgta ttgttggcaa caatccacag acgcggaatc tattttgttg 49980

aaaacctgct gttctgataa gaacatcaat tgttttaact attgtattct atatggcggc 50040

gcccaaaatt tggatgctgc aatggtggaa gcggcaaagc acgatgcccg gatgctgata 50100

aactactgtg tcatgcttgg tggaagatcc ttaaacgaag caaaagaaac ggctgccatg 50160

tttggacaca ttgaatgcgc acaacactgt tttaaactgc agtcttacgt cgtggacaca 50220

tcgaatacag acgacactga ttaaagcgac aatcttacgt catgaacgac tgtcttttga 50280

gtatctatac ttacattata tttttttatg aaaaaaatat aaaggttgta tacaaacctt 50340

tgtatacaag aaatttggat cattaaacaa taattaattt ggacacagga aacgatctag 50400

atcgatcaaa aagctatttt ttttgcacac agaacattta gataattgag agattacttt 50460

ccatacttgt taagcttttt tacacacagg aactttggat tctgttcagg aagtttttca 50520

tagacattat gtttacagcc agtaataata attttgggct ttttcttaaa ccaccggtgg 50580

aaaacatcca gcttgtaaag agggaaatgc atgtagagag gttttggtag tcatggttaa 50640

gagatttgac taactccatg tttcctgtaa agactgccca gtcccaagca gtaaaacctc 50700

tatgatagtc tttttgagtc ggatctgctc caaattttat gagagaaagc atatttaaag 50760

aacggccccg tattgcggcc ttcatcacag gagtcatccc attaaaattc ggtaaacaaa 50820

ttctggtccc attttttccg aaatagccca acaccccttc caggattaaa tgattttttt 50880

tctcagctaa ataatgtaaa gcagagtttc catctttatc cctcctatga gggttaatta 50940

tttctccagg ataagattct tgttcaaaaa gaaattttaa aaagtctata cgtccgtaga 51000

tgcatatcca catgaatacc gaggatccat ttttatcgca tctattgaca atccacggat 51060

ctgttttaaa aaattcctca aatagtgtaa gattcccatt tctaatatgt tttttaatcc 51120

atttaacaaa caagttttct atctcccttt ctggaaacat gtgttccatt ttgaatgtcg 51180

cccctactcc actatatgat tttactcctt taatttttaa tgtccttttt tttcggactt 51240

ctttggataa gctgtttatt accatcttta aatgccttat agcggggagg agccaggccc 51300

ttttcccata tgtgcggtaa ttcttggtgt ttatgcttgc ctttggcata accaggccag 51360

tatttttcga tatattcagg gtttgttttt acgtattctt taaaggtccg ataggcttct 51420

tgaatacagg taggctcacc ggtataattt ccatgttcat cttcctttaa aaagccatta 51480

accctgtcct ttctccactt aagattgtgc tttccaaaaa tgcgatcaag atcttgcgcc 51540

tgctggggtg gaatcataaa tcccttttta ggtcgaagct ttttattttt tccatagctt 51600

cggccatcgc gttgcgaaac agtggttagg acgcctgata gtctttccat gggcgtcgca 51660

tctaatccta tccatccacc ctgatgaata tcaatggcaa caagctctcc tttattttgg 51720

gcaagccaag tttccaagaa tgccatgctt tcttcccagg gataaggccc gccaacacca 51780

cgggttgtcc aatcttgcaa ggactccagg tccgacacct ggtaaggctc taaagaagac 51840

ggttccttgt ttttgtactg caaataagat ttaatgaccc atttatacca tgtgtcgaac 51900

cgcagcgtgg cgcctccaaa gtgaaagccg tcgttgattt taggatatct gcaacatatt 51960

tcaaccgtac gtttgagttc tgcaaaagcg gccttccaag gaagtctttc gctgcgggta 52020

agacggtcta ttttgccctg cgtgccatag cgtatggcat gtcgtgccaa ttgcaacaat 52080

tctgacaccg atccgtgggc cccgatccag tttatcggat aggcaacctc cgaagggttt 52140

aaaagatgct cgtaaaagcg tggatcttca gatgccaagg cgtctgcaaa ggggataatg 52200

ctagaaaacc tgtctagaca tacgttttct gtgtttactt ctaaaggtag aaaaatggtt 52260

gcgtgaggct tttgaacctg cttgttcagc ggtctgcata tgctttgaat aatgtctcta 52320

ggactatgtc gcggcgctgc aaaaaatacc gcgtttagtt ctggaacctc tacgccctct 52380

tgaaagagtc gacagtttaa taaaataacg ggttcctttg aggaacaaaa ttctgtaaat 52440

gttttgagga taacctgtcg cggcagggtt gagtgagcta tcagggcata gaccccttgg 52500

tctaccaacg ccgcgtatag ctccttggcc tgtttaatat cacgggtaaa taccagcatt 52560

ttaggagccg gtatattggt ttttaaatag gctaaggcca ttataatttg ctttactatg 52620

atctgtttcg tggtctcctc tttggtactc ggttggtggg ccaatttagg cgcggctacc 52680

atctgcaatt caaaatcatt tacatagccg gcctctatgc cttctcgcag atagtagcga 52740

aaggcaacgc cgccaaaaag ttcacgattt ttcatggaaa gcggggtgtc gtacctgggc 52800

gttgccgtta aaaaaagtcg gtgccctttt ttaaagttga gcaacacgtg ggtaaagggc 52860

cgtgtctccc attcgccgca aatccggtga cattcatcgc taataataag atcgaaatca 52920

tccaccagta gcgtggagga ttggtaggtg gcaatcacaa gaagagaagg ggcctcccgt 52980

atccgttttg caataaagac aggattggtg gtcatttcta tattgtcgtg atttagcaca 53040

atgcgggtct ggtcagaccc cacaagcaaa acgttcttca aagaaattcc atactgatag 53100

agtttttcca gagtctgccg tagtagggac aggcccggca ccaggtacaa aacttttcct 53160

tgaagataat tggagaggat aagataggcg acgcgagttt tgccgcatcg gcaggccatc 53220

tgcagaatgg ccctcccact tcgccgcagc tcctgatagc ccatattggc cgcctccttc 53280

tgataaagtc gatcctcgat tgcagtccgt gtctcatctg tagaaaaaaa taatacgtca 53340

tctgcgaaat gttcatcttc cacaggagtt atcaccaggt gtctcagttt ctccttgctt 53400

atcagcggat cagagggcaa agatggctca accactatcg tggaatcatt catctcatag 53460

gcgggagaat cacacaaagt atagcttatg tccagacagt ttgcaacatc ctcagccaat 53520

tgttttattt tttcgggtaa aagacatacg agttctttgt ttttgacgcg aaaaaactgt 53580

gcacaatata acacccctgc ttcaattttt tgcgcatcct tctttgtaga tgtttccaat 53640

gtgaaacaat acttccattc atccgtaaaa caggttgtat aagatccatc atgaagccta 53700

gcggccaagt ttcctgtgtg cccaacttta tgtaaggatt gggcctccag ccagggatga 53760

accgccacgt aaaatcctgc gcacatgcta tatcaaattg cagtttctta ataactgtac 53820

acaggatctg aaaaacatgt gattacaaaa tttagataag aaatatttaa tattaaaaat 53880

cacagaatac atgtcactgt gtagagagaa agccaaaaac tcctcttgac cgccgtggga 53940

aatcatccag ggtagtaggt tgtgtttcat aaagttgtat gccgtagtga tcaccgtgga 54000

ctccagatgg ttattggcat ctttgcaata ctttgccatc ttggcagaaa agacgataaa 54060

tccacaaatt ctaccccagt tgataagatc cttaaacagc tcagtcacaa ccccagtaaa 54120

ctgggtttta atttcttgaa cactcgtaag agaaaaggta attgtaacct gtttgttcaa 54180

acactcatca taataggtta aaattttttt tatttgttgt tgatatgggc taagctcatg 54240

ctctgaaata tcattaatgt aatatttaat atatcccact agtatttcat taatgatatt 54300

atgatatatt aactcttctc cctccatagc ggcaccctat atttttttat ttaggtttca 54360

atgttatcac aattgcgata caattgtgat acaattgtga cacaactgtg ttgtatacaa 54420

caaatgttag gccacgtata gcaacctata tgttaagaaa tatttttatc ccaacattag 54480

ttggaaacga gcagccgcaa agaagtcatt taaaataagc catttaaaga tttagaattt 54540

atatgtatac aactgtacaa tggaagcagt tcttaccaaa ctcgaccagg aggaaaaaaa 54600

ggctctccaa aattttcatc gttgtgcttg ggaagaaact aaaaatatta taaacgattt 54660

tcttgaaatc cctgaggaac gatgcaccta taaattcaac tcatacacaa aaaaaatgga 54720

gcttttattt acccctgaat tccacaccgc ctggcatgaa gttcctgagt gcagagagtt 54780

catattaaac tttttgagac tcatttcggg acatcgagtg gtattaaaag gccctacatt 54840

tgtttttaca aaagagatca agaatctggg cattcctagt accatcaatg ttgactttca 54900

ggccaacatt gaaaatatgg atgatctaca gaagggaaat ctcatcggca agatgaatat 54960

caaagaaggc taaataaaac aactaacatc aaaaaacatt aaaggctatg ttgtggacga 55020

tgcctttgtc tcaatagttt cgaggtcatc caataactca tgtaacgtaa aaaagttggt 55080

ccattttttt gaaaacatta aaagacgttc gtcttcataa ataaaaaagt cattcgaagg 55140

aaaaatgata tactcaatac catagtcttg taatattttt tttaggtctc tcagggtcca 55200

gggatttacc aggcttctac gcgaagtgag catcataaaa atatctaata ttttttgcgc 55260

cataagccag cgcggattct cattggccca caaatcaaca ataattctct tatcaaccgt 55320

gagcattcct acttgattcg aagaaatgat tagatgccca gcagtccacc ccatgagtag 55380

ataacgcagc gttgtagaaa tgtcacatat ggaaggcatt cctccacaac atgaacccaa 55440

attaggatgc gtgtgaaaca caaacatagc aggcttgttg gccaccctgc tataaatatc 55500

agcaggcatc atagcctcgc tgccaaaata aatgttctct cctgccctat aggggcttgg 55560

aatgatttcc actatctcgg gtacaccgtt tatcatatta atgcggccgc accattcacg 55620

gtcatcgtcc aaaaattttt tgatggcacc ccgaacattg tcccagttaa gcaacagagt 55680

attcacaatc tcattacgct ccgcccagta ttccttaaaa cttcttttag acttgctgag 55740

ctgttcccag gattcgaact cagtccaatg ttttttttct tttggggaag acttcccttt 55800

tgaaacattt tttgcggctc caccatctac actatgattt tccaaaataa tctccttcat 55860

cgtttgagtt atatgggcat tgctaagcac cttagtggta acctgtttac ctatgtgatt 55920

tagcagaaaa ccaagtttgt ccatttgtgt ctcaaccatt tattcttaac aaaacaaaaa 55980

aaaattaaaa atcatcgtcg tttaaaaaga gtttgaaggc aaacgcatca tccttaacac 56040

agttctgata ctgcgtaggt cttaactcga aaaagttggt tttttctact tcattaagaa 56100

agaatttagt catctgagga aaagggtttc ccaccttata aatgcttttg cactgcatca 56160

tgaagcacaa attatctgta aagtagcgta tatattgaaa tagcatttct tttgaaaaac 56220

cgggaactct tcctcttgcc ttgtcaaagg catagttaat aaactcatcc accaactcca 56280

cagcctcctt caaaattttg tgaatgatct tttcctcggg aatgttatac acgtaatttg 56340

agataagaaa acacgcaaaa ctacagtgca tcccttcatc acgtgagata aactcattat 56400

agcttacaag ccccggcata atattctgtt ccttaagaaa ctggatcgcc acaaagtggt 56460

tttgaaataa aatgccttct acggcggcga agcccaccag ccgctcacct agagtgttcc 56520

tgtcggggtc catccactgc cgcacccact gcgccatttt ttttatgata gggtgttttt 56580

caatgccgct aaagatgcgc tgttgttcct tctcatccgg gatcagcgtt tttacctgta 56640

ttgagtaggc ttcgctatga acgcactctt gggcagcctg cattgtataa aagtataaca 56700

cttcctttac tttaatttcg cgcataaaat tggttaaaag gttttcgata acaatttcgt 56760

cggcaacaac aaagaaggct aaaatttgtt tataaaattc gcgctgtggc tttggcatgg 56820

cttcccaatc atcaatgtcc ttacacatgt ccacctcctg cgccgtccac gtcaaacttt 56880

ctaatttttt ataccagttc caacattcgg ggtgctgaat aggaaaaata gtgaaacgtt 56940

gggaattttc aattagtaat tcctccatat ttgaaataaa tattaacatc ttcaaattta 57000

ttggctgcca tggagacgtt ttttattgag acgttggcat ctgatgtgta tggaaaggcg 57060

ttaaatgttg atttagatag actatcgcag gcgcaggtta aatataccct tcaagagctt 57120

atttcctact gcagcgctct aaccatttta cattatgact attcaaccct tgcggcgcgt 57180

ctttcggtgt accagctgca ccagtcaacg gcctcctcct tctcaaaggc ggtgaggctg 57240

caggccgcac aatcctgctc acgcctgtcc ccccagtttg tggacgtcgt ttacaagtac 57300

aaagccattt ttgacagcta cattgactat agcagagatt acaagctgtc cctcctgggg 57360

atagaaacca tgaaaaattc ttatttgtta aaaaataaag atggggtcat catggaacgc 57420

ccgcaggatg cttatatgcg ggttgccatc atgatctatg ggatgggaag agtggtcaat 57480

atgaaaatga ttctgctaac ctatgacctg ctttcccagc acgtcatcac acacgcgtcg 57540

cccaccatgt tcaatgcagg caccaaaaag ccacaactct ccagctgttt cctgctaaat 57600

gtaaatgata atttagaaaa tttatatgat atggtcaaaa cggccggcat catttcaggc 57660

ggcggcggtg gaatagggct gtgcttgtca ggaatacggg caaagaatag ttttatttct 57720

ggtagtggtc ttaaaagtaa cggcatacag aattatattg tgctgcaaaa tgcttcacaa 57780

tgctacgcga accagggagg cctacgtccc ggagcctacg ccgtctactt agagctgtgg 57840

caccaagaca tctttacatt tttacaaatg cctcgcctaa aaggacaaat ggctgaacaa 57900

cggcttaatg cccctaatct caagtacggc ctatgggtcc ccgacctatt catggaaata 57960

cttgaagacc aaatacacaa cagaggcgac ggcaaatggt acctcttttc gccggatcag 58020

gcccccaatc tacataaggt ctttgatttg gaacggtcgc agcacgaaaa cgcacaccgc 58080

gaatttaaaa agctttacta tcagtatgtt gctgaaaaaa ggtacaccgg cgtcacaacg 58140

gccaaagaga ttatcaaaga gtggttcaaa acagttgttc aagtagggaa tccctatatc 58200

gggtttaaag atgccataaa tcgtaaaagt aatctttcac atgtaggcac tatcacgaac 58260

tccaatcttt gtattgaagt cacaatcccc tgctgggagg gtgataaggc tgaacaaggt 58320

gtttgtaatc tggccgcagt aaatctagcc gcctttatac gtgaaaatgg ctacgactac 58380

cgtgggctca tagaagcatc aggcaatgtc acagaaaatt tagataatat tatagataat 58440

ggctactacc ccacagaagc cacgcggaga agcaatatgc gtcaccgacc tattggcatc 58500

ggggtctttg gcctagccga cgtgtttgcg tctttaaaaa tgaaatttgg ttcacccgag 58560

gccattgcca tggatgaggc catccatgcg gccctatact acggggccat gcgacgatcc 58620

atagaacttg caaaagaaaa aggaagtcat cccagctttc cggggtctgc ggcctcaaag 58680

ggtctactgc agcccgacct atgggttcgc tgtggtgatt tagtttcctc ctgggaagaa 58740

cgcgtggcac agacgacgca gggtgtgttg acgccgaaaa ggtggtcgca gctacgcctg 58800

gcggctatgc agggacttcg aaatggatat gtcacagctc ttatgcccac cgcaacctcc 58860

tcaaattcta caggaaaaaa cgaatgtttt gagcccttta catccaatct atatacacgt 58920

agaacgttaa gcggggagtt tattgtttta aataagtatt taatagacga tttaaaagaa 58980

attaatcttt ggacagaagc cattcaacag cagctactaa atgcgggagg tagcattcag 59040

cacattttgg atataccggc cgagatccgc gatcggtata aaacctccag ggaaatgaat 59100

caaaaaattt taacaaaaca cgcggccgca cgaaacccct ttgtatccca aagtatgtcc 59160

ttgaactatt acttttatga acctgaacta agccaggtac ttacagtgct cgtcctaggc 59220

tggaaaaaag gtttaactac cggttcctat tactgtcatt ttagccctgg agcgggtacc 59280

caaaaaaaga ttataagaaa ctctgagaaa gcgtgtaatg cggactgcga ggcgtgtctt 59340

ctgtaggtgt ctcgcggtaa aagagcagcg gggaccatat ggtaaacccc aacaagagga 59400

taatgaataa aaaaagtaaa caggcatcca ttagttccat attaaatttt tttttcttct 59460

atataatgga atattttgtt gcggtagaca atgaaacctc cttgggggtt tttacttcta 59520

tagagcaatg tgaagaaacg atgaaacaat accccggcct ccattatgtc gtttttaagt 59580

atatgtgtcc ggcggatgca gaaaatacag atgttgtata tttaataccc tcgttaacct 59640

tgcatacccc catgtttgta gaccactgtc caaatcgtac caaacaagca cgacacgtat 59700

tgaaaaaaat aaacttagtg ttcgaggaag agtctattga aaattggaag gtttcagtaa 59760

atactgtgtt cccccatgtt cacaacagat tatctgcgcc gaaactttcc atcgacgagg 59820

ctaatgaagc cgtagaaaag tttttgatac aagcaggacg actcatgtct ctgtaaatgt 59880

ctcttccttt atgggtgacg tctcttcctt tgccgaggaa gtctctgtta tgggcaagag 59940

gtttgaaaca acgcaaggac tctgcttaat ctgctgtctc acaaagggaa tcaaactacc 60000

tgctttcgta tttttaatgt agtaattacc cttgttgtga tgaattttaa gaccatagcg 60060

tagtcccagt actttattaa tgaattttaa aattgtttga gggtccgttt tattgggctt 60120

tttaagctta aactcaaagc tgatcgcgct taaatcatac tgaacaaatt catcaacgag 60180

tttcgtcatt aattgttcat tggtcaatat attagggtcc tgaacgcatt taaagccgca 60240

cttagttaat agcataatag cgtacatatg agattgaaaa ctataattaa attgtagatc 60300

atgatgctct gcgtgttgca tggcccattg atgaaagttt aattcctgag tttgtaacat 60360

agtgagcgac tcgtatactg tctttccgcg gcttatttgg acacggccag tatagttctg 60420

ttttgtcata aaactattgt attgttcaac aaatttggga gtaattttat gaccgtgcca 60480

tgcataaaat tcgagtagtt tatacttttc atacgcaaat aggtcttgct ggtctactgt 60540

gatgccttcc tttaagtttt gtttaatttg taaagcttta ttggcatcaa tggtttcagc 60600

cgaggcaatg tttacatagt cctggtgttt aatttccatt ttaatgcttg tatattgttt 60660

gactgtctcc agcttttcac ccgtcagtat aaacacctta gcgccggtgt cggcgatctg 60720

gttaataaat cgggttataa agtgattttt tgatagatgt tgtatccgca ttgtttcgag 60780

ccatagatgg tagtatggag ttttataata tatcggccta cctgtttcct tactatacgt 60840

gaaggaaagc tggtgattgc ttatggtctg aaaaagggtg tcacgttttt gtaacgtaaa 60900

catttcaatg tcttcgatgg tttctggata gtaattttgt ttcccctgta agcagatttt 60960

ataacactta ctttttaatt cacgcacgcg gcccaacatt tggcaacatg tttctacgtc 61020

acacgacata ttgttaaaaa agccgtataa aacatcaaat ctcttatctt cgtatgaaac 61080

acccgctgaa atcgtgggcg tatagataag gatatcaacg agcccccaat aatacgatac 61140

attattaaaa tgggattccc gttcatgagc agtgctttta gaactataaa acccaatttt 61200

tttttccgga aacttttttt ggataaatga ttgcaacagc cgggcctcca ttaatgaatt 61260

tgtagggata acaatttttt tgtcttctag caaatccttt aaaaggttat ttaaccaagt 61320

ttctcgtgaa gaggtaaaat aatacgtgtc atgctgggcc cttttatatt gattccagtg 61380

aaagaagata gggacatccc cgcgaaaacg ctgtagaata ttatacgttc gatttcctag 61440

gtttgcgtcc aagcatataa cataatttgc cgtttcgagc atccacatga aaatggcaaa 61500

agagggagca aagtatttgt gcaggccgct attgaattga ttaaaaatcg attctacctc 61560

atccaaaata agtaggtcta caggctcggc tgtggaggtt agccggaaaa gtgattctac 61620

ctgaatgatg actctttcgt agctgtccaa atctccagtt acttcgctgt acaatgtgaa 61680

attcggtagc cgggattgta tattttttga gaagatctgt cgaaacgtca caaaccgtat 61740

ggtttgttgt tttgaaatag aattattgcc gtagtatttt tgcaaatagt tgcgcagttg 61800

gacggtttta cctattttca tttgagcctt tacaacaagc gtagggactc gttcatattc 61860

tcgcatacta ctttcatcat agatgtgttt ttgagtatca ggcagttctt caaagagaat 61920

ggactcatga acctctatgc tctttgtcat cacttggtcc acatatgttt ccacaaaatt 61980

atttgtgccg gaaaggctgc ccatgagaag gctatgttta ttgtcatggc gacagtgttg 62040

atacactttg tttcccgtga ctcttaaaat tagggtattg tccttatcat gcatacgctt 62100

acatatttcg cagtaacttg gacttgtacg tttaaacaat actaaatttt tatgaacacg 62160

gaggaagcaa tgatttttac atagtgttcc tgcaaatttt aatacctctt caagttcact 62220

ttgttggata gtatcgcagg aactcggtgt tgtttctttt acatttgtga agatacaagg 62280

taaacacgtc gtttcaaagg gggttgctat aagggtatca ctctttttcg tggttgtact 62340

ggtctcaaac acctctgcaa gctcctcatt aaacatttta acacgcatgc tacctttttt 62400

atgagaccct atgatgcgaa aattttgaat acttttgttg acctgggggt caacaaaagg 62460

ataaacgtgt ttgggaagat tttctaacac tttggatgta aagactttgg cctcattatt 62520

gtttaatact gagtatgtat aaagtatgat atgaaaggag tatttaagtt ctcgcttttt 62580

atttaatccg atagaatctg ttagcaaaat ttgttcacgc gttagattga tgttataagg 62640

taaagaatat gtctcgtaaa atacatccat gatgacgtta attatcatgt caaggatgtc 62700

atagacattg tcttcgacat tatcattgtc atcaacattg tcatcagagt atgacttatt 62760

taccggaaag tcgatgtcaa attttaagcg ctgaggcaaa aacccaaata ccacttcgtg 62820

gaaacacttc tgctcaaagg gctgagccgc ctcccactcc caaaagtcat cacgacttga 62880

aaaaactcta aaaagattat tatattcatc tcgcaccacg aagtgattct ttaaggtttc 62940

gagagaatat ttatcctcta cggcttctcc ttgggagtta cagcgaagaa acttgaatgt 63000

ttcttgcatt ttgatattta aaattaaatc aattatgatg cggccgctaa tgcggcggtt 63060

gacgcggccg cgccgctgac gcagccatca tacataaagc ggcatggccg ttttataacg 63120

actagtcggc cgttatatga cgaactatat aaaaatgaat tcttttaatt agagttaagt 63180

attgttgatt gtataatcca tcatggttga gccacgcgaa cagttttttc aagatctgct 63240

ttcagcagtg gatcaacaaa tggacactgt aaaaaatgac ataaaagaca ttatgaaaga 63300

aaaaacgtct tttatggtat cattcgaaaa ctttatagaa cgttacgata ccatggaaaa 63360

aaatattcaa gaccttcaga ataagtacga agaaatggcg gccaacctta tgaccgtcat 63420

gacggataca aaaattcagc ttggagccat tatcgcccaa cttgagattc taatgataaa 63480

tggcactcca cttccggcaa aaaagacaac aattaaggag gctatgccct taccttcatc 63540

aaacacgaat aatgaacaaa cgagtcctcc cgcctcaggc aaaacaagtg aaacacctaa 63600

aaaaaatccc acgaatgcga tgttcttcac gcgtagcgaa tgggcatcct cgaatacttt 63660

tcgagaaaag tttttaacac cagaaattca agccatattg gatgagcagt ttgcaaacaa 63720

gaccgggatc gaaagattgc atgccgaggg tctttacatg tggagaaccc aattctctga 63780

cgaacagaag aaaatggtca aagagatgat gaagaagtaa tatttttggt aaaaatattt 63840

ttatcaaaat ttttttacca aataataaaa aatatttttt actttttttt cttcataata 63900

tacatagaat gcctacaaaa gctggcacaa aaagtaccgc aaataaaaaa acaacgaagg 63960

gctcctccaa atctggttct tccagaggcc acaccggcaa aacccatgct tcttcgtcca 64020

tgcattccgg gatgctctat aaagatatgg taaatattgc tagatctaga ggcattccga 64080

tttaccagaa tggatcgcgt cttactaaaa gtgaattgga gaaaaaaatt aaacggtcaa 64140

aatgaatata atcaggaaac ttaagcctgg aacaattagc cttgtgctgg gacccatgtt 64200

tgccggcaaa actacgtttc ttattcattg catttacatg ctcgaacgtt tggaaaaaaa 64260

agtagtcttc ataaaatcta ccaaaaacac ccgagacaaa actattaaaa cacactccgg 64320

tatacagcta cgacccaaac aatgtaaaat catagaaagc acacagttat ctgacgtggg 64380

ttctctcacc gatatccatg cagttgtcgt agatgaagcg catttttttg acgatttaat 64440

cacatgccgc acttgggcag aggaagaaaa aattattatt cttgcgggac tcaatgcttc 64500

cttcgagcag aaaatgtttc cgcccatcgt tcgtattttt ccttactgca gctgggttaa 64560

gtatattggc cgcacctgta tgaaatgtaa ccaacataat gcatgcttta atgtgcgtaa 64620

gaacgcagac aagacgctta tccttgcggg aggaagtgaa ctgtacgtaa catgttgtaa 64680

caactgtcta aaaaatacat ttattaagca gttgcaacct attaaatatt aaaaatctta 64740

tacaataatg gatcattatc ttaaaaaatt acaagatatt tatacgaagc tcgagggtca 64800

tccctttctt tttagcccgt cgaaaaccaa tgaaaaagag tttattactc tgctaaacca 64860

ggccttggcc tcaacgcagc tttaccgcag catacaacag ctgtttttaa cgatgtataa 64920

gctagatccc attgggttta ttaactatat taaaacgagt aaacaagagt atttatgcct 64980

gttaattaat cctaaactcg ttactaagtt tttaaaaata acgagcttta aaatttacat 65040

taatttcagg ctgaaaactt tttatataag tcctaataag tataataatt tttacaccgc 65100

tccctctgaa gaaaagacta accatcttct aaaagaagaa aaaacttggg caaagattgt 65160

tgaagaagga ggagaagaat cctaagtcgc ttacattttt ttttgctatt tttatagaat 65220

gtacacgcat gttgatgttg tcggaatagc tgaagcctca gcggccctct acgtgcaaaa 65280

agatagggat cgctacttag acgtgctaac aaccattgaa aactttattt accaacacaa 65340

atgcatcata acaggggaaa gcgcccacct actcttttta aaaaaaaata tttatcttta 65400

cgaattttac tccaacaatg tggcggagca cagcaaggct ttggcgaccc tgctttataa 65460

acttgatccg gaatacctca ctcgttacac agtactcatt accaaaattc ccaaccattg 65520

gtatgtgatt aacgtagatc agcgagaatt tgtgcgccta tatgccatcc cggcagttaa 65580

acaacactta ccgattccca ttttaccctt ctattgcacc agcgcactca cccagcaaga 65640

attgttttgt ttaggacctg aactgcagtt aatacaaata tattccaagc tctgtaaccc 65700

caactttgtc gaggaatggc ctacgttgct cgactacgaa aaaagcatgc ggatgttatt 65760

tttagaacag tttccgcaaa gattggaaat gacgggcggg aagaaggagg agaaggaaaa 65820

gcatgaaagt atcattaaaa aaataatact agaaatggtc tctacccgtc agcgaatcgt 65880

tgttgggggt tacatacaaa aaaacctgta caaccatgta ctcaagaata gaaatcgttt 65940

acagcttatt acgagcttaa atatttatga agaaaaagat atcatccagc aattttgtga 66000

ttcaaatgga ctgaagatca aaatacgtat caacaatccg ctcttgccta caaatccgga 66060

attacggcgt ttgactattt attttaatca taataatgat gatgatcagt catatctaat 66120

agtagatatg tacaacacgg gaagctatga gctagtgcct acaaatcaga taaacacgct 66180

tgatggcagc tttttaatag gaacaccctt cgtgcaagcg cgatttttgt tggtagagat 66240

ctgggtgctt atgcttattg cgcagcaaac taaaaaggac accaaaaaaa taatacaatt 66300

ttttataaat caatatgaaa tgcttatgaa tagtccttgg cccagtatgg aggccctttt 66360

tccctcaagc agtaaaagat atttaggcaa ctatgtagac cctaacgcgc tcataaagtg 66420

ggcacaactc aaattaaaaa gaataccgcc tttttatcct ggaaagccgg atgaagaatc 66480

atgttaagcc gattaaaaaa tcatgttaag ctggttgaaa aatcatgtta agctggttga 66540

aaaactcttg gtgaaagcac ggatgtaata ttaacattgg ccgctcgcat ttcgtgttga 66600

aatacgatgg aagagcgacg gctatctacc atgccgatat cggcctggac atcacagttc 66660

atgcacttgt agatgggatg actcgcgtta tagatggcag gctcgccaca gtttctacag 66720

atgtaggaga tgcagccatc cgagtcgtcg tgcgattttt ctatgatggt ttgcatggcg 66780

ccctgcgccg taagcaccca atgctccatt tctcccagac gaagacctcc gtgcgatcgt 66840

ttgccgtcca acggctggcc tgtgagggca tccgtgggcc catagcttgc aacggcgtat 66900

cggtcatcca gcacaaattt ttgcaggcgc tggtgatagg tcggtcctat gaagatggcc 66960

gcatcaaagt actcgccggt ctggccgttg aacatttttt ggcatccatt gaagcgtaga 67020

ccttcttgcg ccagtctttc tgaaagaagc tgcacattaa taggcaggaa tgcggtgccg 67080

tctgttacca ccccctgtag ggcatttgct agaccaaccg tggtttctat catttgaccg 67140

ttggtcattc gggagggatg tgagtggggg tttacaatga ggtcgggctg caatccgtcc 67200

tctgtgaagg gcatgtctga agtgggcagg gccagcgccg caatgccctt gttcccgctg 67260

cgagaactca ttttgtcgcc tatattgaga tttctttcat agcgcaggcg catgaggcca 67320

aagatctcgt cattaggccc atggggacgc atcacagcat ccacgacggc cggctcatcg 67380

aagccgtaca tgacagaccg gtcgatgtat ttgttgagtt cgtctttttc gccccgtatt 67440

ttggccactt ttcctataat gatgtcgccc tttttgacca ccgttcctac gggcacgaat 67500

ccatctacaa gcttttcgta attagcacca ggcttaagat ttttggtgat taaagggtcg 67560

ggcttcccaa acgactctat atcgctttct aattctactt tttcttctcg gtagaaggtg 67620

ccggcaaagc cgcccctgtc aataaaggac tgcgacacga tcacagagtc ctcctgattg 67680

tagccgccgt agatcatata agccacaatg gtattaagcc cgttgggtat gacatagtta 67740

tgtgctatgg tctttacaag cggcatttca ttgtaaaact ggaagaagcg gttcatgtcg 67800

acacgatatg gccagctaaa gcaataccag ccccccgttt gccggccttg gtttgtttca 67860

taggtaacac gcgcaggttg ggtacagttt gcgtaggggg acactagggc ggcaaggccc 67920

aaaatagctt ggggcacgtc cacgtgtgtg aaacgacgcg ttacatcatg tttatgtttg 67980

cgtagctcga tgatggagaa ggcaacaaga cagttttccg cctcctcggg ggtaatgaac 68040

tcacagatgc cctgtgctac gagatcttca agtgtaagcg ttccggctaa aatgtctttt 68100

gccatttgag gcgtaaatcg cgtattttga atgaaaggga ttttatgttt ttcccagtct 68160

ttatcgcctt tttttctggc ctctgcggcc ttgtagcagg cttgattgta tttttcaata 68220

ttattatcta caatgagtag ggggcgggtc agcctaccga cgtccaacca aaattctact 68280

tcgtctacca tgctatccca gtagatggtg gtatggggat gcacaacctt gccctcacgg 68340

cgaagcattc tataccgctg agcaagctca aaggcattgg tgcagcagcc gatccattct 68400

ccgttgataa atacgcgcgc taggcccttt cgtacaatgt ccttgttgga aacatcggct 68460

aactgttgaa tggccggatc tgatagaagg cgttgtttta acgaaagtac ttctccggcg 68520

gtgcagacat tggcagtgat ggctaactgt ttagacatgc ctactttttc accagtatcg 68580

gctgactggg ctacgcagat gtatccagga taggatgcgt gcacgcgacg catcatgtca 68640

gccctttctg tttgtttgga tgcgttggtg gtgttatgag tatttaccgt acgcaatgct 68700

gaaatggtat ttaataaatt ttttctttcc aaactttgag tagatactct gtttacaatg 68760

gggcgctgtc gcaccatgat ggttttattt cctgaaatga tagactgttc catactgcga 68820

ttaagatcgg aggcggtatt ttttgataaa gcggcagaaa atgcctcgat aatgtttcgc 68880

tgagtaagct cctcaaaggc tgtttgttta agaagttctt tgaacccatt gatgatgggt 68940

gctatcacgg aagtattaaa aatagcctta aaggccttgg cgagtgagac ccctgagccg 69000

tgcacccgct tggtgcggta gctatcacgg tccgtgggtg gaaacacatt cataatgaca 69060

agaagtattt tatgaataag caggcctaaa aagcgcagct ttcgtacacg tgtatctgcg 69120

gtttggccca tgtgtggcag caatattttg tctaaaatag taagttgtct ttcatttaag 69180

tattgtaccg cattttcatc gcttttgtaa gcagatgggt ttgagacaaa tttggaaacc 69240

ttctcggata aaaactggat aattttttct cggttcagct cgtgttggac cggttgaaat 69300

atggggtcta aaacatgaat ggatttttcc agaatttcta tcatgaaggt attcacaagg 69360

gagttggatt ctagatcaaa taccacttgc tcaatgatgc tgtcatcgcc tgtcattcca 69420

aacatgcgaa agatgagata ccaaggtatg cgaagttttg agaacttggt gctattgatt 69480

tcaatggtaa tggcgccggt ggtcatgtag cgtataataa tttgagagct attttcgaag 69540

gcacctcccg gttgggagat aaactcgccg cgaatgattt cattattccc ttgttgcatg 69600

gtatggtaat ggatgtgaag cgtgttaaag cggatgtttt ctaagaggtc tacgacccat 69660

tccccgcctc gggctataaa gtagccgccg ggttcattag ggtcttctcc tatttctttt 69720

tttgcggttt ttgataggtg atgagtgtgg cagcggttgc tgccccgcat gatgggaaat 69780

gtagatacct gaaaaggagg aatacttgct cgttttacct cctgccgacc attgctgtag 69840

tgcgccgtta aaataacctc ggcggctaga ttaaccgggc ccgaatagga aaggccacac 69900

aggcgtgcct tattgggtag taaatttatc ttgtttccct gtgaatagtt tcgatgttgc 69960

gggcgttcaa tgttcacatc tgtaaagtta aattggatct gaactgattc ccgaagctta 70020

tctatttcag tatggtcgcg ttggtcttta taagtaatat ccacgttaaa catttgtttt 70080

acaatttgcg gaattccatt gtccataaga tcgtcgaagc ttttgatgtt ataccctatc 70140

aatcctgtag agtttactgc agcggagata aagctcagca tatcagcctc tgtaagctcc 70200

tcattatcca cggtttcaat ggggccgtag gttatttgcg gccgcaaggg ttccatgatt 70260

atgaagtact acattaatat tcagttattc tttaaaataa atctttattt ataaatctta 70320

tttataatat aagaatgcct tatgcaagag acatcacaaa gtttattacg gcaacggaac 70380

cagaggtggg tcttcccctg ttggcgctgc agcgctccaa atccatcata ggggttattc 70440

ttcttgtaat aagtttgtta tttattttca ttggcattat tatattatca gtgagtagtg 70500

gtcataccac agcagcctct atatttatcg tattgagtct tatcctaggt ggcggtggtt 70560

tttttcttat ttataaagat aattcttaac ccacataaaa tttgaaaaaa tatagagtaa 70620

gaaaatgtcc aattactatt attactatgg cggggggaga tatgattggt taaaaacagt 70680

agaacccact aattttttaa aaatcgggtt gccttaccag gcacacccat tacatcttca 70740

acatcaggca actactcccc catctatctt agaaaaattt aaacgagcag acattcttct 70800

taatgaggtg aaggccgaaa tggacccact catgttacaa ccagaaaccg aaaaaaaact 70860

attccagata ttgagtagta ttgatatgtt caaaggtctg cgaaaaaaag tagaattcac 70920

gtacaatgct caaattgtta cgaatgcttg gcttaaaatg tatgagctgc taaataccat 70980

gaattttaat aatacatctc aggcattttg caattgtgag cttccaggag ggtttataag 71040

tgcaattaac cattttaatt atacaatgat gcattaccct acttttaact gggtagcttc 71100

ctccctttac cccagttcgg aaacagatgc cctggaagat cactatggtc tttatcagtg 71160

caatccggat aactggttga tgcaatctcc tttactgaaa aaaaatatag attataataa 71220

cggggacgta accatcgcta gcaatgtaaa aaacctagcg cttagagcca cacaaaggct 71280

gacgcccatc catctatata cggctgatgg gggtattaat gtaggacatg actacaataa 71340

acaggaagaa ttaaatctta agcttcactt tggtcaagcc cttacgggtt tgttgagtct 71400

tagcaaaggc ggaaacatga tactcaaaca ctatacctta aatcatgcat ttactctttc 71460

tttaatatgt gtattttctc acttttttga ggaactatac attaccaaac ctacctcctc 71520

tcggcccaca aactctgaaa cctatattgt gggtaaaaac agattacgct tatttacccc 71580

caaggaagaa caagtccttc taaaacggct agaatttttt aatgatacgc ccctcgtaga 71640

cctaagtctt taccaaaatt tacttgaaag cgtttacttt gccgtagaaa caatacatct 71700

aaaacaacaa atagaatttc taaacttcgg aatgaaatgt tatcgacatt tttataacaa 71760

gattaaacta cttaacgatt atttagctcc gaaaaaaaag atttttcagg ataggtggcg 71820

tgtgcttaat aagctttatg ttcttgaaaa aaagcataaa cttaagcttt gtgcctccta 71880

gggatctgtt gcttaattta acagatgcaa tcttaacaga tgtaaactaa aaagtgtgtt 71940

catacaagga ttgtatttat gaatatttat taacatataa ggttgtgatg taacactgta 72000

taacctatat aactacacta tgaagcacgg cgtataataa tttatattga acacgatgtt 72060

gactcattta tttgcaaaca aatatttgtt tgcaagacgt ttgcatgcat ttactaatat 72120

gttgttgact agtttatttg caaactagat gtttgattgc aaactagatg tttgcacgta 72180

tttatttgaa ctaatataca ctccttgttt tatttgttat atacacagca tacataagtg 72240

tatattgttt acacttatgt ttataactcg acgtaataac attttacacg cttttttttt 72300

gcaaatctta ataatattgt atgataaatc aaacaatgtc ttatatatgt ggtttattat 72360

tttaggcgcc gcaagatgta ctccattctc attgcatgct tggtgttatt actctgtcta 72420

gttatatatg tcggtcatcg tgccgatcat gcacgaaaat atttagaagg aatgtggcat 72480

ggagatccgg tttttctaaa acagtcgggg ctacaatcct tttatctcta catacaacct 72540

gaccatacat gtttttttag cattgtgaat aaaaatggtg aaaagctgat ggaaaccaaa 72600

ataccttgta cgataacaaa taaaatatat atgtttttta aacctatttt tgaatttcat 72660

gttgtgatgg aagacataca tagctacttc cctaagcagt ttaactttct gttagatagt 72720

acagaaggta aacttatttt agaaaacaat cacgttattt atgctgtatt gtataaggat 72780

aatttcgcca ccgcactagg aaaaacggtt gaaaaatata taacacaaaa ttaatcatgt 72840

tttctaacaa aaagtacatc ggtcttatca ataagaagga gggtttgaaa aaaaaaatag 72900

atgattatag tatattaata attggaatat taattggaac taacatctta agccttatta 72960

taaatataat aggagagatt aataaaccaa tatgttacca aaatgatgat aagatatttt 73020

attgccctaa agattgggtt ggatataata atgtttgtta ttattttggc aatgaagaaa 73080

aaaattataa taatgcaagt aattattgta agcaattaaa tagtacgctt actaataata 73140

atactatttt agtaaatctt actaaaacat taaatcttac taaaacatat aatcacgaat 73200

ctaattattg ggttaattat tctttaatta aaaatgagtc agtactatta cgtgatagtg 73260

gatattacaa aaaacaaaaa catgtaagtt tattatatat ttgtagtaaa taatattttt 73320

aattacttaa aatttttata tataagtttt tgatactata ttataaaaca tatgttcata 73380

aaatgataat acttattttt ttaatatttt ctaacatagt tttaagtatt gattattggg 73440

ttagttttaa taaaacaata attttagata gtaatattac taatgataat aatgatataa 73500

atggagtatc atggaatttt tttaataatt cttttaatac actagctaca tgtggaaaag 73560

caggtaactt ttgtgaatgt tctaattata gtacatcaat atataatata acaaataatt 73620

gtagcttaac tatttttcct cataatgatg tatttgatac aacatatcaa gtagtatgga 73680

atcaaataat taattataca ataaaattat taacacctgc tactccccca aatatcacat 73740

ataattgtac taatttttta ataacatgta aaaaaaataa tggaacaaac actaatatat 73800

atttaaatat aaatgatact tttgttaaat atactaatga aagtatactt gaatataact 73860

ggaataatag taacattaac aattttacag ctacatgtat aattaataat acaattagta 73920

catctaatga aacaacactt ataaattgta cttatttaac attgtcatct aactattttt 73980

atactttttt taaattatat tatattccat taagcatcat aattgggata acaataagta 74040

ttcttcttat atccatcata acttttttat ctttacgaaa aagaaaaaaa catgttgaag 74100

aaatagaaag tccaccacct gaatctaatg aagaagaaca atgtcagcat gatgacacca 74160

cttccataca tgaaccatct cccagagaac cattacttcc taagccttac agtcgttatc 74220

agtataatac acctatttac tacatgcgtc cctcaacaca accactcaac ccatttccct 74280

tacctaaacc gtgtcctcca cccaaaccat gtccgccacc caaaccatgt cctccaccta 74340

aaccatgtcc ttcagctgaa tcctattctc cacccaaacc actacctagt atcccgctac 74400

tacccaatat cccgccatta tctacccaaa atatttcgct tattcacgta gatagaatta 74460

tttaatatgt actatatatt aattatttaa cctttcaagc tggtcttcat ttaaatttaa 74520

aatccactaa taaaatgtat tttctagtag cagatcatcg agaacatcat gtgattcctt 74580

ttcttaaaac cgatttccat cacatgcatc aaaatcctat acaaaaaaat caagctctcc 74640

tagaaatcaa acagcttttt actggagatt atctcatctg caaaagccct tctaccattc 74700

tggcctgtat tgaacgaaaa acctacaaag actttgcggc ttctttgaaa gatggacgtt 74760

ataaaaatcg ccaaaaaatg ctgtcgctgc gagaacaaac caactgtcaa ctttattttt 74820

ttgtagaagg cccggcattt cctaaccctc aaaaaaaaat taatcacgtt gcctatgcaa 74880

gcattattac tgctatgacg catcttatgg ttagagatca tatttttgtc attcaaacga 74940

aaaatgaggc ccacagttcc caaaagcttg tgcagctttt ttatgccttt tctaaggaaa 75000

tggtgtgcgt cgttcccacc tccctcaccc ccacggatga agagctatgc atcaagctat 75060

ggtcttctct ttctggtatt tcaggcgtga taggtaaaat cttggcaaac acttgttccg 75120

tagctcattt ggttcatgga aagctttcat cgcagaatat tgatcagtta aaaactccct 75180

ccaaccgacc attccccaaa aaagtaaaac gtatgcttat aagcattagc aaaggaaata 75240

aggagttaga aataaaattg ctctcggggg ttcccaatat cgggaaaaaa ttagctgccg 75300

aaattttaaa agatcatgcg cttctttttt ttctaaatca gcccgtagaa tgcttggcaa 75360

atatacaaat cgttcaaaaa acccgtacga ttaagttggg aatgaagcga gccgaagcga 75420

ttcattattt tttaaactgg tgtggctctg cccatgtaac cgatgatagc caaaatatca 75480

cagaggcgtc gcggtccaca atgcaggtcg cgacgcagtc cgccgcaata cagcccgctg 75540

caacgcagcc attgcacgaa gtatcagatg atgcatcatc agatgcttca tcacccgtag 75600

ggtatcaaac attatctaaa gaaatgttat tgaacacagc ctgatgttaa taattcacta 75660

catctaaaga aatgttaacc tcgatactaa aaagtcattg aacacaacta ctggggcgct 75720

aagttgtcca acacatctaa agaaatgtca acatcctcga tgctaaaagg gtcatcgagc 75780

cggtcaataa tgtcttcccc aaaaagtccg ggagaactgt aggccgagat gtcgtccatg 75840

gagctatctt ccccagagca cacaaagtcc tctccaaaaa tcataaagtt aaatgcaccg 75900

ggcttactta acagcttttc gctttgaata atagtgttga gttctgtcag cgcaaactct 75960

ctcacaatat tcacaaccca ggagggctct ttaatttcat acagcgttaa gaaacttata 76020

cataaaaatt ctatagagta aagcaaggcg ctggcaggat ctgttacccg taggtgttta 76080

aatgtagtgt gatattcatt cacaacgtta ggcagcacct tttccaaatc ctccttttcc 76140

tcgtacgaca ggtgctttac aagcctttca acatgtatag gaggcttgtt aaatgtacta 76200

acgtgccgca aacagttata attatataag aaaatacgta cggcagagtc gaccgccatg 76260

agccttggat catccattga ggtaggtggt ggcggggcac cctggccttc cctgatgtct 76320

gcgtaggagc gcccctccat ggcccctatg gcctctatca cagcaggact gatatccaaa 76380

atcttggccg tcttgattat ttttccgtaa tcgaaagtcc atggctcctg tggaggcttg 76440

ggttgtgttt cggtggaggg cgtggtcata tctttcttta tttgaataga acggatcgac 76500

atcttttcct tatcgtactg gtctttataa ttattataat agtcatgaac taattcgggt 76560

tgagaaagat gatcgtatat aatataggta aaaagtccgc acttgacaca ttttttatcc 76620

tggaagtcgt gtaatcctcc cttggggcag cgtgactcgt agaaggcata aaaggtgtta 76680

aattctaagc tcgcctttag ggctgtttgg acctttttta tgtttaattg ccccacctca 76740

tgttgtagca cgtggcatac agaacagcgt agatcggcaa gtgcataatg gttgtcaatt 76800

ttttttatga cgtctttgcg tgttacttca atctcggcgg gtttctgcga actgtctacg 76860

gccttgtaaa cgtaaatggt ccacttatga ggaagccccc tttcatcgta tagggttgaa 76920

atgggaagcc ttttatactc aaacagccga gtccgttggt cggctcttcc tgtgttagga 76980

tcaaatatgt tataaaatcc ttgctgagca agcagggcct tttgctcgcc ataagcattt 77040

tcgtacgttt tgaattctgc aagttcggag ttaaaattag gtgcattttg taaatactta 77100

agaaataatt cataggctct aaggtaaatg agagttgagg ttttttcctc atcccgtcct 77160

ccccaccaca cccgcaggct ttcttcttga aaatagatgt cattcagacg cgtcaactgc 77220

gtaaaatcag gccgatattt agaggtataa attttatcat aaaattcttt ttgcgataat 77280

agctcggccg gggtacgtcc tatcacggtt ttaaactcat attcagcctc cttgggagtc 77340

cgtggtttgt gcatagggat gctgccgtca atacgggcca ctgtggcagc ataatcatac 77400

atggggtcca gcagaatctc tgtcaaaagt accttggtgt cgtcctgcac gctaagccct 77460

tgtagcccat tttggtggat aatttttttg aaagcctccc gaaaattatt agcaatccac 77520

tgatccgtaa tctcagatag ctgatttatt ataccgctat attgctgcat cattttctcc 77580

aaaagaaagg tcacgtatgc attcaaagag ctatccgcct tcattccatg aatggtaatc 77640

gtaagaaatt ctttattttt ttgcgagcta taaatgagat tcaaaatata ggcatagatg 77700

tagatcacag catacagctg cgttaaagga tcgtaatcct cttccttttt aatattttcg 77760

atgctataca cgagcggcag gcagacattt acggctatat tggcaaactg tttcacgtct 77820

acaagctttc caaagtggat aaacgtgcag gccttcatgg tttcctgcca aataaaaaca 77880

cggagcttac tattaagatc gccgatgatg cccacatctg ccgtacgatc ctcttgaata 77940

aaatgggcca gctcttcgcc acaaattttg caaaagtagg agtaaataag cccctggttg 78000

ttttctttct ccttgtttat tcctgaaaat ttcattagct tggttcgcat ggtgtcgtag 78060

gacgcttctg ccgcttgaag ctgtataagc atgtccacat ggggacaaag cagcttaaac 78120

ccgcaggctt tgcatagatt ccaattggtg gtattgtttt tttccttgta gagtacacga 78180

atactttcta atacttttaa taactccgcg tattgaagac ccgaacgcaa ctgttttacc 78240

agcttgagat gagcacatgc atttttttct tggagttccc actgtttttt aatgtttagg 78300

tattctgttg taataagttc tgcctcctgt ttcccacagg ctttaatgac ttcttgaagg 78360

atgctgttag ggtcatccac tttaccctcc attgtaagaa tttcacgtat agcatccgac 78420

tgcaccctac ctattttttc ttccataatt ttaaaatact gtctcgcctg ggtaatgacc 78480

tctgtgagct tcatgtccac ctgctgcaga atcatttgct ccttttcacg ctgttcagca 78540

tgttgtaaaa acttttgttc tacagggttc caaagcacct ccaaatagcc tgctctatat 78600

aggtcataaa gcaagggcat gtatcccgat gtaaaaaccg gggacaccga gtacatcgta 78660

gacaactctt ttaaaaaaaa tatcacgcgc ttaatgttct cctccggttc aatctcctcg 78720

gtttcaacga tattagatat atgactgccc tgatcctcac ggtctagctt tcggtgtacc 78780

atctcctctg ctagccgatt aatgagccag ctatgcccgc cgctccgcaa aaacttataa 78840

agttcgatat actggtgcgt aaactggatg atgttttcct tggtggttac gacaacccct 78900

tctccgtttt ttttccaggt ttcttgatcc acgcatttca taaatactcg aataaaattg 78960

gtcaaattgg ctcctgaggc gacgtagccc aaggtttcag gcgagaagga gcctatctca 79020

gccatacgca taaaacactg cggggaaaaa gtttttagcc gcaacttaag tccatagatt 79080

tcaatggggg cttctgcggg aacggccagg tgcgtcccat taattaaaaa aatttctttg 79140

cgtgtgctag ggcgaacacg taattccttt tttttttcac tcacgatggg gaccacatcg 79200

gggtctacca gcagttgacg tatgtaggcc tctatgggca tggatagatc gggcagcttt 79260

gactgctcgg cgcgaacatg gttcacaaaa tcttttagag tgaaaagaaa gtctattaaa 79320

cgtatgtttt ttatatcatt agacccttta agggtagagt agatttcatc cactagtgcc 79380

tcgatttcct cattattgag cgataagata tctgtgccac ggtggactat ttgcgcgatc 79440

gtaattactt cctccattag atagaaactg aatattatat ttaaaataaa tacaaaatgt 79500

caaatgaaag ttttcccgaa acgttggaaa acttactttc aatgttacag accaaacagc 79560

aaaacgcaat tcagtcagag gtgattgaat ggctgcacag cttttgtgaa acctttcact 79620

taaaaataca ctgccataaa cagtttattc ctagcgggga aaaaaaacga gctaaaatac 79680

ccgctcaaga aacacaggga aacacgcagc cctcccacca tgtgtaccgg gttgttctct 79740

ccagagcaca gccagtcaaa gcacaggaat ctctgctaac aaccatgtgc aacggactgg 79800

tgctagatgc aaacacatgg acatgcctag ccattcctcc gcctgcgccc tttcaacagg 79860

cgacccgcca ggtccaacac ttttaccgta acaatttcta cgaagtggtt cccatccagg 79920

atggcaccct tctcacaatc taccactggg atgaccctga atatggcccc tcctggtgcc 79980

tagcaagtac ccacggatat gatgtgagta actactgttg gataggcgac aaaaccttcg 80040

ccgagcttgt atacgaattg ctgcagcagc actctacctg cgacgtcacc ctggaaaaaa 80100

ataaaacgcg gggaacgcgt cttttctttg ataacttaaa tcccgattac tgctatacga 80160

ttggaatccg gcaccataat ttacagccgc tcatctatga ccctcaaaat atttgggcga 80220

ttcaatctac aaacctaaaa acgcttaaaa cggtatatcc agaatactac ggctatatag 80280

gcattccagg aattcagagt caagttcctg agcttcccca gtatgattta ccttatctaa 80340

tacgatctta taaaactgct atgaatcaag ccaaaaatgc tataaaaaat ggcaaaaaag 80400

acaagggata ctttaattat ggctatttac tcatttcgcg agcgcctgcc attactaaaa 80460

gtacttctaa tgttttgtta aaatcgcctc tgctggtatt tttacaaaaa agtgtgtacc 80520

agaaaaaaca caatatctct aacagccagc gactagaatt tattatactg caaaactact 80580

tgatgcagca ttttcgagat catttcattg ctctatttcc gcagtacata tcctattata 80640

cgaaatacca aaacatgttg aatatgatta tccatagtat tgcaactaaa gataaagatc 80700

atccctttgc aggagccgtg gtaaaaaaag tgttggaaga tattgaaaac gccgaaaaca 80760

ttattgatca tacaaccatt caaaactatg cccatcaaag caagtacgcc atgctttact 80820

tgtcaattat ttcccatttt taatctaata cggccaaagc cgcgggtttt ttaataaact 80880

aacatttaaa aaaactgttt tattaaaaat tataatactt ttattatata tggaacatcc 80940

atctacaaac tatactcccg aacagcaaca cgaaaaatta aaacattatg ttttaatccc 81000

taaacacctt tggtcttata ttaaatacgg aacgcatgtc cggtactaca ccacacaaaa 81060

tgttttccga gtcggtggct ttgtgcttca aaatccctac gaagccgtta taaaaaatga 81120

ggtaaaaaca gcaataagac tgcaaaatag ttttaacaca aaagcgaaag ggcatgtaac 81180

gtgggccgtc ccatatgata atattagcaa gctatatgcc aaaccagatg caattatgct 81240

taccatacaa gaaaatgttg aaaaagctct tcatgcttta aaccaaaacg tactgacgct 81300

cgcatcaaaa atacgttaaa tataattttt gtagaggata aaaagctatt ttagctaaaa 81360

aataattcat atacgtttat gcagaggaag aacggtggct ttcaaattca gattgcatcc 81420

acgtagaccg tagcgttttt tttgcttctg gtttatatcg taaaccgtaa taaacatcat 81480

catttgtatc cgttggatct ttttcccact ccggataaaa aatcggtttt cttttttttt 81540

ggtcgttttt tgcagtaagc tgtaaattaa gggaatatag cttatcgaaa agttgttcct 81600

gatccatata aatagcagca tatattaaaa aaaaataaaa aaagacgctt caacgagtca 81660

gtaccactgc ttgccaacga tttacgttgg ttggtgcatt atggtgatat agtaatgagt 81720

gcctgcacaa gtgcttgcac aagtgcctgc acaagtgctt gcacaagtgc ttgcacaagt 81780

gcttacacaa gtgcttgcac aagtgcctgt acacattact gcatcgccaa agcacctgca 81840

atgcctactt cctcaacaga gtacgataac taaatgcttt taagcaccgc ttgcgtcgat 81900

gtgtccttcg gggcaatcgg gttcaattgg atccaatatt attagtcata attacctaat 81960

acttattcaa ttttatcttt tttaccttgt aagatttaaa cagcgtttta gcttgtttaa 82020

agcaacgttt aaaacaagct aaaatgctgt ttaaaacaac gttttaaaca agttaaaaca 82080

aataagctta taaatatacc atgacaaaat tagcccaatg gatgtttgag cagtatgtca 82140

aagatttaaa cctaaaaaat cgagggtccc cctcgttccg caaatggctc acattgcaac 82200

cctcactgct gcgctattcg ggtgtgatgc gtgctaacgc ctttgacatc ctaaaatatg 82260

gctatcctat gcagcagtca ggttatacgg ttgctacgct tgaaatccac tttaaaaata 82320

ttaggtcttc ctttgccaac atttactgga accgtgatag cgaggagcct gagtacgtct 82380

gctgttgtgc cacctatcaa tcgcacgatg gcgaataccg gtatcgattt gtttggtacc 82440

aacccttcat agaggcttat aatgccatag aggcggccct ggatcccctg gaaaccatta 82500

tcctgaacct cattgcggca cgagatctag acttcgttgt tcacatattt ccttataata 82560

agggccatga agactatttg gcctccacgc aacttattct caaaatcttt attgcgacgc 82620

ttttaatgga cattttaaga attaaagaca acacgttgga cgttcactta aattccgact 82680

atattattgt gatggagcgg ctttggcctc acataaagga tgccatagaa cacttttttg 82740

aagcccataa ggacttacta gggtacttaa ttgcctttcg caatgggggg aactttgcag 82800

gaagtcttag accctcctgt gggcaaaaga ttgttcccct aacgattcga gaggtcctac 82860

aaatgaatga tattaattta gccgtatggc gggaggtgtt tattatgcag gaatgttccg 82920

acttagtcat caatgggata gcgccctgtt tccccatttt taacacgtgg acgtatttgc 82980

aaggtattaa ccagattttt tttgaaaaca cgtctttgca ggagaaattt aaaaaagatt 83040

ttattgcccg agagctttcc aaagaaatta tcaagggcca aaaaacgttg aatgacaagg 83100

agtttaaaaa gttaagccta catcaaatcc agtacatgga atcctttcta cttatgtcgg 83160

atgttgccat tatgattacc acagagtatg ttggctatac ccttcaatcc ctgccgggta 83220

ttatttcgcg atccagctat ttatccccca tcgtgaaaaa cattttgatg gacaaagact 83280

cttttatgtc cctactattt gacctatgct atggcgccta cgtgttgcat aaaaaagaaa 83340

atgtgattca cgcggatttg cacctgaata acatgaccta ctaccatttc aacccaacca 83400

gttttacaga tcgcaacaaa ccaggaaaat acaccttaaa ggtcaagaat cctgtgattg 83460

cctttataac cgggcccaaa gtcgaaaccg aaacgtacgt gttcaagcac atagatgggt 83520

tcggctgcat cattgacttt agcagagcca ttatggggcc aaaccatgca atcaagcttg 83580

agcggcagta cggcctcgct tttgtaaaca ccttttaccg caatcaaagt gagcatattt 83640

taaaggtatt acggtactat tttcctgaaa tgctaaccaa tcgcgaaaac gaaatacagg 83700

gggtgatttt atcaaacttt aatttctttt tcaatagcat tactgccatt gatttttacg 83760

ccattgctag aaacctacgt agtatgcttt ctttggacta tttacacacc tctgaggtga 83820

aacgaaacgt agaaatttcg caaacatttt tggatacatg tcaatttttg gaggaaaagg 83880

ccgtggaatt tttgtttaaa aatcttcata ctgtcttatc tggcaagccg gtcgaaaaaa 83940

cggccgggga tgtgctttta cccatcgtat ttaaaaaatt tttataccca aatattccta 84000

aaaatatatt acggtctttt accgtaatag atgtatacaa ttataataat ataaagcgtt 84060

attctgggaa agctatacaa acgtttccac cctgggctca aaccaaagaa atcttgacgc 84120

acgccgaggg tcgtacattt gaagatattt ttcctagagg agaattagtt tttaaaaagg 84180

cttacgcaga aaacaaccat ttggacaaaa ttttacagcg tattcgtgag cagcttgcta 84240

atgaaaattt gtaaggcttg cagttcttgt atggtcagaa cctatgtcga tggaaacatt 84300

atttttcgct gcagctgcgg cgaaagcgtt caaggggata gtcagaactt gctcgtctct 84360

agcaaggtgt accacaccgg ggaaatggaa gataagtaca agatttttat taaaaatgca 84420

ccctttgacc ccacgaattg ccaaataaaa aaggattgcc caaattgtca tttagactat 84480

ttgacacaaa tctgtattgg aagccaaaaa atcattatat tggtgtgccg ctgtggctat 84540

atgagcaaca gaggataaac catatcatcc caccgaatta tgacattcct ttaaaaccgt 84600

ccgcctaaat agttttcaca cctttggtgg cagactattt tataaaaagt aatgttggtt 84660

catgaagata aagtgtgcca aagaaacttt tataaacaaa tgattaatgt aggtgctagt 84720

cgtgtgtact taaacagggt attctatagc caagtatttt ctatagccaa gtattttcta 84780

tagccagtat tagtcaagta tttagatgtc agggtatttt tatagccagt atttttctat 84840

atgtacaaac tattccagta aacatatgtg tgttctttat tgagcagcat catggcatta 84900

acaagtttat taaactgctc taatgggcat taaatgacaa ctcggtgctt agcaaaagtg 84960

cctatacctt ttaacaatta gggccgggag gcattcccag cttttttcta taatcagcca 85020

tacagtaccc ctgagcctca tacacgggaa taaggtcctt ccattccttg ttgggatcgg 85080

cgggccagct ctcaaatgag gtgtgaatgt aagggtcctg ttctttttcc ttaatgaagc 85140

gtttaatctc catttgatgt tgtttacttt tttgtttgcg gcggagcgtg ttccgcacca 85200

atacgtaaaa aataccaaga atcacacata aaagaattat taaaaaaaat atcatcatcg 85260

cggggtttaa aaaacgatcc catgcaacag gaatcgttct taaaaccttg tctggcaggg 85320

ctgtaaacat gaagtctcct cctataatcg gggtgggact gtagcctaac agttcaaggt 85380

cctgtcgttc tagatactta ttggcgaact gcccaccctt tgcccccgtt tttttattaa 85440

tcaagcagcg ctgcattttc caccattcta aatcttcagg agaaagctca atgccatata 85500

tcaactttaa cgttattgca tctttttcaa tatccttatc aatttggctg agcttttgag 85560

ctttaagcgg gtctagtgtg tacttccatt taaacttagt gtcctgtagt ttggctacat 85620

gaaatacgga acatttcggc ggggcctttg tgacgccctt acactgcgga agtttatcat 85680

taggacaggc gcatagatga gactgcgcca cagcatcgcg aactacatcg cagacggagt 85740

acattttcct cctatgttaa acaataaatt tttttcatag ctgaaatttg tgggcctatc 85800

ttttcccttg cccggataat aattataagg gagtgttgaa acatctggga gagaattgct 85860

taaaaaatgg gtttttggga ggggtaactg cgactgttgt acgtcgttgg ccagggagat 85920

tctatatgcc gggctaaagg tgcaacgttc ctgtgaacaa cttagtacgc gcgttgttaa 85980

tacaaatgga ctggtattag caaacctcgt aaactcttcc ggacttgttt gtttttgtat 86040

gatgtttagc agggagtctg ccttttcgag aatccaaagc gtcgcattgt agtaaaataa 86100

aaatagcgac ttatcggcag gcgttgcaaa agcgccgtat agaaaataaa gcagtaagta 86160

ctggggagac accacaataa ggttatcttg aatgatagat atcgctagct ctttaaacat 86220

agtgctaaaa aaatgtatgt cgttcgtctt gaatataggg ggactatagt ccatgtaggg 86280

ctcacatatc tcagtcaggt gaaggcccat ttcttttatg acttcttccg ggttgtacgt 86340

cgctaacacc agcgcgggat aggctttggg catatccacg gtaagtgtta tgtttttatc 86400

attcttatgg taggagtaag atggttgtgg aaattctgtt ttccactccg ggactttgca 86460

ggtaattctc agctcattta gagtctggta caggagggcg tatgccgcaa agccgtgtat 86520

ggccacttgt ttaaagggaa ttgaaaacgt tttactttcg tatgtcgact tcacaggaac 86580

aacgggaatg gggtaatatt tttctatgag gttataccgc tgcaaatcct ttttaaacct 86640

gctaaaaaca tcttcccttg gtgggttatc aaaaggaaag caaaatgcta ggtgtagccc 86700

ggcccgctgg taatcggggt gaatgatttt aaggttttta tacgttaatg tgggtatggt 86760

gttaaagata ttggggggca tatatgaaag atcagcaacc cacacaaagt ccgtgcgcac 86820

ccgcatggtc tgcacatgga tggcgcgcac cgtgcccacc tgcttgaagc ccttttcata 86880

caaaatgtca gcaagttcgt aggcgtcctc aacgtggttg ggggaaaaca tatcaaagtc 86940

gggtctttct ccctcgggat aaattgagct gcctttaaga tgcagggcat aatcaatggc 87000

aatccccccg tacaaaataa gctttttctt tatgataaat tcgcggacca cctccaaagc 87060

cgcctcaatc tccacggcat ttgcctcacg tttttgagca atgagccggt acttagaaac 87120

attaaaatca gtctttagta aagacgtcat aaatagtgtt taatatatat taaaggtttg 87180

aataaaatac taaatagtaa aaatggatgc cctattaaag gaaatagaaa agttatcgca 87240

gccatccttg cagaaagaaa acaatgatgt atgcgatctc tgttttatgc aaatgaaaaa 87300

aatttctaac tatcagcttt tatgcgaaga gtgcggtcag ctgaaggact ggtttgaacc 87360

tgaatataat gaaaaattca cggtatattc tcgtctaaag atcgtgggtg ccaatagttc 87420

ctatcaccag cgcgatttgg acaaggccaa ctcaagtgac tatagctcct tgcaatttca 87480

tcacatttta gaggagctca aatccctaaa tgttaagtat atggatgcgg ggcaaaagcc 87540

ctttcctatt caggtgttaa aagaaactgc tcacagttat aaccaagtac aacaacatcg 87600

ggtcatacgc agcattacaa agcttcagat cttagccagt attctacgta gcatttgttt 87660

aaaattaaac attgcttgta cggtggcaga cgccgcgagg tttactcaac ttaataccaa 87720

agggatctca aggggcatgg atcttctgcg ctccctattt gtagacaata aaattacttt 87780

aaacgttgat ttaaacccta tagacagctt tattaatagt acctacagtg ccttacaaat 87840

taaacaaatc caccaagaac tgcaggagga aaatgtttat aatttaaaag aaattgttaa 87900

gagctttata ttatacgcgg atgagaagaa catcggcgtc gatcttaaca ggagaaccgt 87960

tgtgattgct acgatgtata atgttttacg ccgtgcctac taccccatag aaattgatac 88020

ggtggtgtat caatgtaaaa tacgaaaaaa tacaattaca cgtgctctta aaatgtatga 88080

ggattactac tcccacttta agtctcttta tgagcagtat catttaaacg cggcaaaaaa 88140

attaatttaa actaaacgtt taaactaaat gtttaaacta aacgttaaaa ctaaacattt 88200

cgactaaagt ttaaaaccta gtctaacagc gggatgccca tttccctggg gttccatatt 88260

tcaacaattt tttgaccttc gggtgttacc ttgatgcagc gcatgacgag cagtggaatt 88320

ttcctattaa agagttcttg cttagctata tcaataggac tgctatattt ttttttaagc 88380

attgtagatc cattaattgc caattgttgc gctctaacgg cgaccaacct tgtggcctca 88440

aaggtggtta aaacgttgga ggtaatgcgc tcgttatcgg gtataatgac caatgtttgc 88500

gacgaggcct gcacaaagcc ctcgcagatg gacggagact ccacgatctc gtccttgtcc 88560

tcggactcct cctcactgtc gacgaggttc tcctcttccg tttccacata ttcctccacg 88620

aggtcatcca tgataagatc ctcgttgtca ttatcagcca tattacactg ttatcaaatg 88680

tactgtttaa tacgcaaatg gatttactac gttttaattg tatgtcttca tgtgcaggct 88740

ctagtggaaa gtaattttct cacaattttt ggcaccgtta cacttgtgcc cacaaaaacc 88800

cgcgattttt ttattttata ttacttttgg aagtacgagt ttaaccagtc gctttcaaac 88860

cttatgcgtc tatctcgcca aaaaacgctc acagcggtgt tggatattac ctttaaaaaa 88920

ataacattaa tttttaccac agagggcgta ttgcgtatgg attctacgaa taagccaggc 88980

gtgccactcg atatagaccc ccagttcatt gaccttgata gtattttaat ggaactggat 89040

cattaggacc tctcccgccc atttaaattt ttagtttcta caataataaa atgcgcgagg 89100

aatcatggga agaccacgat accattcagc tcaccgctca gcgcaaatac ctcgccgagg 89160

tgcaagctct agagaccctt ttgactcgag agctttcagt ctttctcaca gagccaggca 89220

gcaaaaaaac aaatattatt aatagaatca caggaaaaac ctacgcactt cccagcacag 89280

agctactaag actctacgag catctcgagc aatgtcgcaa gcaaggcgcc ctcatgtatt 89340

ttttggaaag acaggggacc tactcgggtc tcatgttgga ctatgacctt aaactcaata 89400

caaatgctgt tcccccgctg gaaccccccg cgctatcacg gctttgccat cgaatatttg 89460

tgcatataaa aaacagcagt gtgctgcctg agggcagcca taaaatccac ttctttttta 89520

cattaaaacc tgaagtggtt cagggcaaat atgggttcca tgtgctcatt cctggtctca 89580

agctggcggc ttctaccaaa aaaagcatta taggatccct acagcacgat gccaccgtac 89640

aaaaaattct acacgagcag ggcgttacaa atcctgagtc ctgtctggac ccccactccg 89700

cctccgttcc ctcgctcctc tacggctcct ccaaactaaa ccacaagccc taccaactga 89760

aaaccggctt tgagttagtc tttgatagct ctgatcccga ctacattccc attcatcaaa 89820

taaaaaattt agaatcttat aatttagttt ctgagttgag ccttacgaat gaacagggaa 89880

gccttgtaag acctgtctat tgcgcggcag acattgccgc tgagaaggag gaagagatcc 89940

cgaccgagga tcactcgctc tccatattaa tgctacatga tcccgaagcc cggtatttac 90000

ataaaatttt aaatctgctt cctccggagt attatgtaga gtacccccta tggagcaacg 90060

tcgtattcgc tttggccaat acatccgcta actatcggcc cctcgccgaa tggttttcgc 90120

aaaaatgccc tgaaaaatgg aatacgggag gaaaagagaa actagaaaaa ctttggaatg 90180

atgcctcgca ccacactgaa aagaaaatca ccaagcggtc cattatgtac tgggcccaca 90240

aacatgcccc ccagcaatac aaagaaattg tagaacaagg ctacttttcc attctcgctg 90300

aatatgtgta tagctataac ggcatgcttg agcactacat gatcgccaaa gtcatctatg 90360

ctatgatggg caacaagttt gtagtggacg tggattcaaa cgggaagtac gtttggttcg 90420

aatttgtgct accgggccag ccaatgaatc agggagaaat atggaagtgg cgcaaggagg 90480

taaacccgga tgagctgcac atctatattt ccgaaaactt ttcaagggtg atggaccgaa 90540

tcacggagca catcaaatac cacctcagtc aaccccatga aagcaatatt ttaaattatt 90600

ataaaaaact attaaaagcc tttgaacgct ctaaaagtaa aatctttaat gacagcttta 90660

aaaagggagt tatcaggcaa gctgagtttt tatttcgcca aagaagcttt attcaaactc 90720

tggataccaa tccccaccta ctgggggttg gcaacggggt tctctccatt gagaccatcc 90780

cggctaagct cattaatcat tttcacgagc atcccattca tcagtacaca cacatatgtt 90840

atgtgccctt taatcccgaa aacccctgga caaaactatt attgaatgca ctccaagaca 90900

tcatcccaga acttgatgct aggctgtgga tcatgttcta cctaagcacg gccatatttc 90960

gcggcctgaa ggaggctctg atgcttttgt ggcttggagg cggctgcaat ggaaaaactt 91020

ttctaatgcg acttgtggcc atggtattgg gcgatcacta tgcctccaag ctcaacatca 91080

gccttcttac aagctgcaga gaaaccgcgg aaaaacccaa cagtgccttt atgcggctta 91140

aggggcgggg atatgggtac tttgaggaaa ccaacaaaag cgaggttcta aatacgtcgc 91200

ggctgaagga aatggtaaat ccgggcgatg tcaccgctcg agagcttaat caaaaacagg 91260

aaagctttca gatgacggcc accatggtcg ccgcgtccaa ctataacttc atcattgaca 91320

cgacggacca cggcacatgg agaagactgc ggcattatcg gtcaaaggtg aaattctgcc 91380

ataaccccga ccccagtaac ccctacgaga aaaaggaaga tcctcgcttt attcacgagt 91440

acatcatgga tccagactgc caaaacgcat tcttcagcat actcgtctat ttttgggaga 91500

agctacagaa ggaatacaac gggcagatta aaaaagtgtt ttgtcccacc attgagagcg 91560

aaacggaggc gtacagaaag tcacaagata cgctacatag gtttatcaca gaaagagtcg 91620

tggagtcgcc ctccgcagaa actgtgtaca acctatccga ggtcgtgacg gcctacgcgg 91680

aatggtacaa caccaacatt aacgtaaagc gccatattgc cctcgagcta tcccaggagt 91740

tagaaaactc tgtgctagaa aaataccttc agtggtctcc caacaaaacg cgaattctaa 91800

agggttgccg tattttgcat aaatttgaaa cgctgcagcc cggcgaatcc tacattgggg 91860

tgtccacggc cggcacactc ctaaacacac ccatatgcga gccaaaaaat aaatggtggg 91920

aatggtcccc taatccctct gcccctcctg agaaagaagc gtctgcacca actccttagg 91980

gaatatcctt agaagcatgt ctttcggcag agccattacc ggtagcaaaa aagcaacatt 92040

gagtatatta tatgccttag cctgctcata agcgtccttt tttttcatgg tattttatgt 92100

ttttaaatat ttttaattat tttttaaata cgatgaacag ttcgtgctcc gaaggctgtt 92160

tactaaaaat cggtgtgaat ccgcattctt taaatatggt ttcccattcg gggatggtat 92220

ggaaatccat gtctctacga atagtatggt gcccaagtgc gtcctgcagg ctgtgaagcc 92280

agaaggcctc ctgaccttga tgaaggtcgt acatgataag aaaaccatca ggtttcaaca 92340

gatggtaaag cttgttaaaa tcgtttatcg taagatgatg cgccgccata ggtaacccta 92400

tgagctccac agagttttca tgctggacat cgtccatatc ggtataaaac gtttcacagt 92460

aaatgagacg cttaaacgag tatcgatgac aaacatttat ttccaagtag gtttgcacta 92520

cgtttttagg tatatcggga atcatgttga ttaaggttgt ttcgggaaac ttaatcatct 92580

gactaggctt cattttcaac tctttaaagg atttcccgga gaagtgaaaa tgggtcttta 92640

cgtatttatg taaaaatacc tgaatgggca gagggggctc ctcctcttcg ttctcgacgc 92700

ctcccaaaat atttggaatt tcctgacgtg gcaaaagaaa gtttatgtcc acgtttacga 92760

atccatcgag gacggacaca aagcttggct ctaatctcca ttccatatac tgtttagaaa 92820

cgggagatag cataatccta ggcgtcacaa tgcacgaagg gtttttaatc accgcatcgt 92880

ggtaagaaaa gtgtattcca tttcttccag tataaagaag cctatgttcg tcgtagcaga 92940

aacaattaag gcggtatgcc tcatacatac actgtttcaa agtacaaaca cgttttaaaa 93000

aggtttctgc attggcggag gccaagcggt tttgccattg gtggaagggg ttcaatccta 93060

caatggccag ctcgtttaaa atatcttcgc ggcgcgctaa aatctgcacc atagaagaat 93120

actttagcat ttttttttcg caccattcgc gaagatgttt agctacatta ttaaccttat 93180

tattgataaa gtatacgatg gcatgttgga agccttcaaa aataaagagc ccctccaaaa 93240

gatcatctgc caatagaaga tggatgttgg tgtaagcatt gtcaatattt tgtagaaacg 93300

gcggaatgcc tgccaaaacc gcttcagcaa gcatagctcc gttccgttgt ttactgtcca 93360

atagattcgt aagttttttg tccgcaacag acacgacggc taggatggtt gcaatgtcag 93420

aaatggcggc ttgccagaaa taacccgaaa agcacatgcg cgcttcttct atagataaaa 93480

acgaaaagcg agaggcaatg tctccgagct gcgtgagttg aagacctttt tctcctctgg 93540

ttaaaaggcc tgccacaatg gcccgctcaa tggctgatgc cagcgcatcc gtggggggag 93600

gatccagcat atcaatctcc tctgccttaa acacgccttc cttatttttt ttaatcgttt 93660

ctacgacaat gctaagaaaa atggccccag ggccttccgt aatgatttca ggatactgct 93720

gcactggtat ttgctcaaag acgtgttttg tgtaaagcgg gtaaaagtgc ccaggaaata 93780

ctctccctac acgccccttt ctttgctcga tacggctttg agccgcgggg cgcgtaataa 93840

gccctcccgc ccattcggga tagtaggttt caatgcttct gttccacccg ggatctatga 93900

cgtacttcag cgtttcaatg gtaaggcccg tttccgcaac aaccgtggaa acaatgaccc 93960

ttcttaaagg tttttccact ttagcggtta agggattttt cacccacaga ttcttaattt 94020

ccgctttcag gccaaggtag gcctcatttt cctgcgcaat cgcctcacta tcgatcggca 94080

aaatcaacat taacggcagc ttttctttgg caaggtccat atttgcatta ttcagcaaca 94140

tcgaaaggaa gcgtatttca gccataccgg gcatgaaaat taaaatatct gcttccgtgg 94200

gacgatcatg aatgttttct ttatgaatag tgagagccgt ttcgcaggcg gtcttaatgt 94260

agttgttggt gttatacagc ggccagtggg tttccacacc gtactgtcgt ccttccacca 94320

aaataatgtt ttcttttccg ataccaaaat aggttgagta tttatgggta tcaatggtgg 94380

cggaggttaa aattacaaag ggaatacgca gcgcccctat gcttcctctt tgcaacatgc 94440

gctgaagcat acttttaata tacatgagca taaggtcgat gcctagggct cgctcatggg 94500

cctcatctat aatcataaag gcatagcggg aagctatctc atcatccgtc attgtatgta 94560

gctgcgccaa cagaaccccc gcggttgcat aaataaggcc ccgattgggt ttttccgtca 94620

gaggcttcgt ttggtagccc actgtttggc ctaatatcat gtcggggtag tgggttgagg 94680

cgccgatgtc tttggcgagg gtcaccgcgg ttaggactct tggctgggta caaataaccg 94740

agcgtcccaa gtatttttgg aaagaatgcg tgttttcatt tctcagaatt ctgaacacgt 94800

gtacgggtaa ggccgtggat tttccggaac cagtgcgtga ctttataatg agcacccggt 94860

ctgcgaggga ggttggaatg gcccctccaa actccgggag acgttgtttt atccaagtga 94920

tgatgtaatg aataggaaca tcattcttgt gctcagcggg cacgttatag agatgaccag 94980

gctccaataa agtcggtttt cccatattct attgttttaa ggattgattg ttcataaata 95040

tttttatact ctgaccaaga aattattttt ttattaagcc ggttatttac gttgttatgg 95100

aacgcgaagg tccagtactg aaagtcctcc gagttgttta atgtcaaggg attttttgta 95160

agatacgaaa aggcgtggtg ctggcacctg gtgcatggca gagactcgat aaagttcagt 95220

atccattgga tggcttcata tttttctttc cagctaggag cgtctgaaaa aaagatagca 95280

tatagatgca aggatcgcca gtatttaggt ccccaatgca acatttataa ccttttgaaa 95340

aatctcattc catatagagg taaatatttt ttttccatgg agaatttttt tgcactcttg 95400

aagggattgc gccacatcgt caaatgtttt ttgttttcca tgtattttgg cgtaattcca 95460

gccagtatct gtgtcatggt ccttaatgtc atccgctaac tgaaaggcat gtccaaaaca 95520

atgggcagcc ctttcaatca tcccaatgtc ttcaacggat ccagttccta aaacccagcc 95580

cataataaac gcgatcttaa aaaagggaat ggttttttct ggagtgtcta ctaactgacc 95640

ggaacccgcg ctgtttagag agtggcttac aaaggtacac agcagcgctc ccagttggtt 95700

gggatccgga aaccttggac agtgttcctt aatccagtcg atttgccggc aaatattttg 95760

aaatccttgc atggttagcg ccagagcgct catctgcgcc ttggctacgc caaagcgggc 95820

ccacactgta tctttatttc gccgcttcac atcgttgtca aaggagggca tatcatcgat 95880

aatcaaagaa gctacgtgaa agtactccgc tgctagggcg gcctctgccg gataaatagg 95940

cgccccaaag gaatgttgca actgacaggc ccgaacaatt tccatcagga taatgggacg 96000

gatatacttc ccacctctta gagcgtaaga gcaaggctct gttagttgtc ccttaaagtc 96060

cccatcttca atagcattat ttaagatggt ctcaaactct tcactaaagg ttttataatt 96120

tttaggattc agtggatgta ttccatgaaa aagcgcgaca ctacgcggtg ctgtgattct 96180

aaaatactta ggtttgcgcg tataggatat taaaataata ataagaacta caatgatgga 96240

gatatagatg agatgcaaca tgctgagttg tctccccgca gggaatggtc cttttccgcg 96300

cttgttaacg gtaccgagga ggcgttgaaa tctttaggaa aggtgctgtc tagtttggaa 96360

tctccaattc ctcccgtata tttaggtata taattattgt gtctagaaat tgtttgcttt 96420

gaggtatcaa aatattcagc ctgaccgcta tttcttttag aataattcgg tatagggctt 96480

gagtagttgg caatactctt aaaccggggc accaaggtaa caatattttc catataatgg 96540

gtttgatacg ctttgtttaa aaatgggctt accggcttta tgcttgttag ttgtgcattg 96600

agtaccggta tgtcttctag gatttgtggc tttatagaat gattagcaaa cacagaatgt 96660

agtatattag atacttgtag catatgtcta tttgcggaaa attcctggta ttctctgccg 96720

tgttgcgaat ctttgggcgg aaggggacca agcatcggca cgtccgtgta ggtactggtg 96780

gattttatga gttcctgctc tatgttcggt ttgacatgtg gatttcctaa aggaatacct 96840

ctacctgcaa tccctttttc taccgacgca ggtagattgt gcgctaaaca caaaatattg 96900

tacacgtctt tgtgcggaat atatccgtta tagtgctggc ccggcatctg atcgccaagg 96960

tgctgctcat gcttaatggt accctttgtt ctgagtttag gaagatcctc gtacgaaaaa 97020

aattttgtgt gctcgctgaa cctcgtagaa ggaaccgaac tattttttgg gttttttaag 97080

gaaggcaatg aggaaggctg ggtcagacaa tttttctgtg tgccctttaa gctagccacc 97140

tgcggaaatg tttttttttc cgtacgaaca acattgcgcc taattaggtt ttccgtatgg 97200

gttgaaaaag caggacgatg atttttaaaa tgattaaaaa gtttattttt tggaatggag 97260

ctgtacggct ccagatcttg cgcatcgccg taaccaatgt ttttgtgctg agggttcagc 97320

ataaaagaaa agttacgtag atcactgagt tgcaatccct tttcagcctt ttcaggacta 97380

ttagtgtatt cattgtatac aggcgcagct ccatttttgt tgccgcagta ccgggaattt 97440

agtatattat cagaataccg gttatgacgc ggcaaatcgc tttcccaaag aggtggatct 97500

gacctataat cggctaacag ctttgaagca taatcatgat acattgtata taaaagttaa 97560

ttattatatt gagaaggcat aattacttct tgtaggggta caagaggctt tgaatcaggc 97620

aaactgacgg gttttgaatc ggccggcttt ggaccggcag gtatcttttt aggttgatct 97680

tcttctagct cattagacac ggatggggga gaaataggag gaataatttc atctccgccc 97740

ttatatttgt catggataga agaaacaatt acatccatgt ttgatttatt ataaatgtcg 97800

tttaactggt gatttaaaac ataataatgc aaaaataata gggctacaat gcatatatat 97860

acgtaaatag ccgtcttcgt ttttcgtttt ttatccaccg gcggattaca aattgcaaaa 97920

aatacaacta ataccaccgc tgtaatgatt aaggccacaa tgaaaggatt ttgaaaggat 97980

gttttgaacg gttcgcacgt ataaattttt tctcctaaat tattgatacc cgcaataaaa 98040

tctacattca ttttatatat ttataaatta tgaaaaattt agagttacat ctccgccgga 98100

ccaatcattg ctaaaatttg aagattcttc aaaaaggccc gactggttga atgtcttctg 98160

ctcaggtttc caaaaatttt ccaagaatgg attttgaaca ataggctcat cttgattttc 98220

ttcttcaagg atattttctt tgatatcaag aacagcttct ttaaactcag gtgtatcttg 98280

attaaactca ggtttatcct gatcaatcgc aaaaatatta tcttcttcag atatatcctg 98340

tttaatcgca agaatagttt cttcctcagg tttatcctga tcaatcgcaa gaatattttc 98400

ttcttcaggt ttatcctgac caaactcaac aatatctttc tcgctaaatc cgtttttagt 98460

gtgaagctct tggttttgaa gagaattatc aaaatctatt ttagttgttg tcctagaccg 98520

tggcacggga tagttatcta atggtttact tactatagtc ctcgaatgtg gcacgggata 98580

attgtttggt gacttgctgg ttagctcttg gcttgttaat agttcttgtt ttctcaataa 98640

ttccatctct actacttctt tttgatccgc tggtgtctct ttttggtatt cttcattaga 98700

aaaatgttca gagggtaatg tttcaataaa ctttgtgagt ggatagctgc tctttgatgt 98760

agaagagcgt tgaatttgct gataaaggag ttgaacaagt cgccggtatt cactctgtct 98820

tttttcatat tttttacgta gcgtggagag atctgctaag agcgacttgt tttcagatgt 98880

taattcttca atttgatgaa gaaggctgcg attgtatgaa ctaagtcttg catacgtttc 98940

ttctaattct gtctccggct ccacataggc ctgttttcgc agaaatttat tgtatagttc 99000

cattcttttt ttgagcagaa aggtaagact ataatcttgc atttctttcg taactttatg 99060

gtagttttct ttccggtttt tgataataaa gggcagcatt ttttctgttg tgataaaggt 99120

gcccagattg ctaatgtagt cgcacagtag caattccaag atagattctt tcttttcaag 99180

gcttatagat tggctgtatt ctttaggtat gaaagaatca acaatcgttg ttacgaagtt 99240

tgaaaagttt aatgttttgc tgttaatttg ggtaatgtta caaaaatatt tgtaaaaact 99300

atctagcatt ttttcataaa gttttttatt ttgtttaacc cctaaaatat agccctttac 99360

ttgatactga tattccgtaa caatggaatg ttttttgtat agtgcatttt tgtataaaaa 99420

gttataaaaa atgttgataa aatacgcacc aagggtttca aaaatactta taacgtggga 99480

ttcttcctga tccattatat catatgtaat attattttaa taaaaaatta ctgacgaata 99540

acatgcaaaa aaaatatgtt taaacttatt ttaagctagc acttatttaa aagtgtttta 99600

aacacgtttt aaattgtatg ttaatacact taaaaattaa gccgaaattt gctccaataa 99660

ggattacttt tatcaatgac cacctcttta ctataaacgg ctttacataa ttttaataat 99720

gctttagagc caaagctgaa ggcagtggga agcggcactg tactatggta aaaatgttgc 99780

cgatgttcat cctcgcggat gtacacaagt ttcctatatc ctttaaacac aatatggcta 99840

atttcttcca catactcctt atcctgtttg gaatagcggt tgctttgacg ggaaaaattc 99900

gacatacaaa tagaggcatt tgtaaaaatg gaaacaaatg cgtttttacg aagattggcg 99960

ggtaaatcgg tatcatcttg gcagcaaata atcatcgaaa taaaacagtg acgattttgg 100020

taaaaaaact ttttaaaaat ttcttttgta aataatgggt gcagttcggc cgcgcagtcg 100080

tctaatatta aaagtaaacg aggattaaga ttgatatagt ttaacgtaaa cttttcatcc 100140

tctgtaaggc ataagttttt atacatatga atgttctgta taataatttt ttttaaaagt 100200

tgctgataaa gcgatgtaat cttttcttct tttttttggt ccgtttgttc agcctttaag 100260

cactccactt ttgcaatatt tttgttttcc ttttgctgta tatcgatcgg aagtttatga 100320

tacaatgttt ttagcatatc gatgttgttt actcgactgt agatggagga catcatagtt 100380

tgccgctgcc agatggcctc caaaaagcgt tcagcgccct tgttgtcatt ttttttttgc 100440

ttatcggcga gccacaagcg gtagtgtatt agagttggat gtacaaaacc ctcatatgaa 100500

cgatttgagg gttccgaggg ggcaaccact aaaatttgtt caatatgggg ttgcaggatt 100560

ttcataatat gtttaacgta cacggttttg cctgtttttg aggggccata tagcacagtt 100620

gttttatcta taaaatgatg tgctttgaac tgtagttcag gaattagctt ccctgaatgg 100680

gtcgttaggg ccatctctat attattacaa ttctgctttt gtatataaaa tttctttttc 100740

gagtttatta ttattgttga cccacatatc tacccgtatc gtatcatcag gcacattgag 100800

catttcaagc gcattatcta actgtttttt tgtttttatc agctcgcttt cttcatcggg 100860

ggttaaattt tctttactaa gcagttgctt aattttttct tcgcagtcgt ctataaaatc 100920

atactctcga gcttttttga tatttccaga tgctttttct aggtttttta gctccttaaa 100980

ggaaagcagt cccttaatcc cgctatccgt gtgaaaggtt gaattataga tggagagccc 101040

cggagcatcc gggccagttt cttgtatatt ttttgctttt ttgtggtaaa tagtatttcg 101100

taaaatctct tttcctatct ttaggtcttc ctcatgacgg tccaaaatcc gttttattat 101160

ttcattattt tgattaaaat aattgtagcg ctctctgttg gccttaaagc ttcccaggag 101220

tgtccagttg cctaattgaa tggatgaaac ctctgagaaa atctggtctt tatatttata 101280

ataaaattca tcaacctttt gttggttgct gctatccacc acatcataaa taatgaaggc 101340

aaactctagg tcgggttttt ctgggtagat gctttccgta gcggcccgca actcttcgta 101400

attatcctca atgtaataat tccacttata aaaagtatcc tgaggtggaa tatgctgcga 101460

aagatatcta gtaatttttg tgttaaagag aatgggttta aacgccctcg gattttcaag 101520

catatgttta atgctttggt gaagttctat attttgtaat atgtgggctg ctgccctata 101580

gccctgtggg gtttgggtga ttgcatcaat atcggcctga agctcattag gcacatttaa 101640

tgttttttgc atgatgtgta aagggatgcg ctcaggatct gctaaatcgg tgtattctgt 101700

gcttgtacaa gtgcttgcac aggtatctac attggtatct gcacacatgc ttgcacaggt 101760

gtctacattg gtatctgcac acatgcttgc acaagtgtct acattggtat ctgcacaagt 101820

atacgcactt tgagcatgaa gattaggatc aaacacaaaa tgttctcgta aaaagctatc 101880

gatcgttgtt ttagcttcct tgcttttctg cgtctgggtt ttgcagctat ctgctataga 101940

taaaattgta tttactaccg attcagaggg aacatcatta gtttcctgtt tcaaagtatc 102000

aactaacgtt attagctcac tgagaagagt tttggtcgtg tgggtaggtt ttgaatagga 102060

aggcatccat tcctgcagag ctttgaagac atatccaata aagctagtca ttataagacg 102120

tcgaatatac tgctcccgca aatttgtaaa agagcaaaag gccaccctgc tatcattttt 102180

gaactgtttg taagggttcg tcctttggta aagctgttta agcgtttctt cggatatttc 102240

agtagaggga tcctccaata cgtttttgag aagctcatca atattaaatt ctgccatatc 102300

ttagagttta ttatatacat attaaagctt taatataagg gggggtataa caatggacga 102360

aatcatcaat aaataccaag ctgttgaaaa actttttaag gaaattcagc aaggattggc 102420

cgcgtatgat caatacaaga ccttaattag tgaaatgatg cactataata atcatatcaa 102480

gcaggagtat tttaactttt taatgattat ttcaccttat cttattaggg cgcatagcgg 102540

agaaacgctg cgaaacaaag taaataatga aattaaacgt cttattttgg ttgaaaatat 102600

caataccaaa atatctaaaa cgctggtaag tgttaatttt ttactacaga aaaaactttc 102660

aacggacggg gtgaaaacga aaaacatgtg gtgcaccaat aatcccatgc tgcaggtaaa 102720

aacagcccac aaccttttta agcaactatg cgacacacag tccaaaactc aatgggtaca 102780

aactttaaaa tataaggaat gcaagtattg tcataccgac atggtgttta acaccacgca 102840

gtttgggctg caatgtccta actgcggttg tattcaagaa ttgatgggaa ccatttttga 102900

tgaaacacat ttttacaacc atgatgggca gaaagcaaag tcaggtatct ttaaccctaa 102960

ccgtcactat cggttttgga tagaacatat tcttggtaga aatccagaac aagagttggg 103020

gaccaaacaa gatccctgcg gaaccaaggt gttgcaacaa ctaaaaaaaa ttattaagcg 103080

cgataataaa tgcatcgcgc ttttgacggt cgaaaatatt cgaaaaatgt taaaagagat 103140

aaaccgcaca gacttaaata attgtgtttc tcttatattg cgtaaactta ccggagtagg 103200

gccgcctcaa atatcagagt cgattttact acgaggcgaa tacatattta cagaggcaat 103260

taagatacgg gaaaaagtgt gtaaaaaagg gcgtattaat aggaattatt atccgtatta 103320

tatatataaa atttttgacg ccattttgcc tccaaatgat accacgaatc gacgcatttt 103380

acaatatatt catttgcaag gaaatgatac gctagctaat aatgatagtg agtgggaatc 103440

tatctgtatg gagctccctg aaataaaatg gaagcccaca gatcgaaccc attgtgttca 103500

ttttttttaa agatgaagat tttttagatg atttttttta gttttttaaa agacgaaaaa 103560

attttttaaa agatgaatat tcttaaaccc cgcaaattac ttttttttag gtactgtaac 103620

gcagcacagc tgaaccgttc tgaagaagaa gaaagttaat agcagatgcc gataccacaa 103680

gatcagccgt agtgatagac cccacgtaat ccgtgtccca actaatataa aattctcttg 103740

ctctggatac gttaatatga ccactgggtt ggtattcctc ccgtggcttc aaagcaaagg 103800

taatcatcat cgcacccgga tcatcggggg ttttaatcgc attgcctccg tagtggaagg 103860

gtatgtaaga gctgcagaac tttgatggaa atttatcgat aagattgata ccatgagcag 103920

ttacggaaat gtttttaata ataggtaatg tgatcggata cgtaacgggg ctaatatcag 103980

atatagatga acatgcgtct ggaagagctg tatctctatc ctgaaagctt atctctgcgt 104040

ggtgagtggg ctgcataatg gcgttaacaa catgtccgaa cttgtgccaa tctcggtgtt 104100

gatgaggatt ttgatcggag atgttccagg taggttttaa tcctataaac atatattcaa 104160

tgggccattt aagagcagac attagttttt catcgtggtg gttattgttg gtgtgggtca 104220

cctgcgtttt atggacacgt atcagcgaaa agcgaacgcg ttttacaaaa aggttgtgta 104280

tttcaggggt tacaaacagg ttattgatgt aaagttcatt attcgtgagc gagatttcat 104340

taatgactcc tgggataaac catggtttaa agcgtatatt gcgtctactg gggcgtccag 104400

ctataaaacg tgactggcgt acaaaaagtc caggaaattc attcaccaaa tccttttgcg 104460

atgcaagctt tatggtgata aagcgctcgc cgaagggaat ggatactgag ggaatagcaa 104520

ggttcacgtt ctcattaaac caaaagcgca acttaatcca gagcgcaaga gggggctgat 104580

agtatttagg ggtttgaggt ccattacagc tgtaatgaac attacgtctt atgtccagat 104640

acgttgcgtc cgtgatagga gtaatatctt gtttacctgc tgtttggata ttgtgagagt 104700

tctcgggaaa atgctgtgaa agaaatttcg ggttggtatg gctacacgtt cgctgcgtat 104760

cattttcatc ggtaagaata ggtttgcttt ggtgcggctt gtgcaaatca tgaatgttgc 104820

ataggagagg gccactggtt ccctccaccg atacctcctg gccaaccaag tgcttatatc 104880

cagtcatttt atcccctggg atgcaaaatt tgcgcacaag cgttgtgaca tccgaactat 104940

attcgtctag ggaatttcca tttacatcga atcttacgtt ttcataaagt cgttctccgg 105000

ggtattcgca gtagtaaacc aagtttcggt acgcattctt tgtgccgggt acaatgggtc 105060

ttccaaaagg atctacaagc gtgtaaacgg cgccctctaa gggtgtttgg ttgtcccagt 105120

catatccgtt gcgaggaaac gtttgaagct gcccatgggc ccccatctgg gacgtgccct 105180

gaatcggagc atcctgccag gatgaatgac atgcacccaa tatatgatgg cccaccatat 105240

catggaaaaa gtctccgtac tggggaatac caaaggtaag cttgtttccc aaggtggggg 105300

tacccgtatg cgggcgtact ttattgtatt caaaccctac tggaacataa ggcttaaaat 105360

gcgcattaaa atgcaccaaa tgtgtttctt cgatttgact caaagtgggt tcgggatcgg 105420

gtttcccata acttttgttc acatttttaa tgttagagat cctgctattc agcaagtctt 105480

gggccaatat aatcttgtcg gccttcccat cgttagcaat aagacaaaaa gctcctcctg 105540

atgccatata taatgttata aaaataattt attgttttta ttaaatatgg cggtttatgc 105600

gaaggatctt gataataaca aagagttaaa ccaaaaatta attaacgatc agcttaaaat 105660

tattgacacg ctcttgctgg cagaaaaaaa aaactttttg gtgtatgaac tacctgcccc 105720

ttttgacttt tcctccggcg accctttggc cagtcagcgc gacatatact atgccatcat 105780

aaaaagcctc gaggagcgcg ggtttactgt caaaatatgt atgaaagggg atcgtgccct 105840

ccttttcatc acctggaaaa aaatacaatc cattgagata aacaaaaaag aagaatatct 105900

gcgcatgcac ttcatacaag acgaagagaa agcattttat tgtaaatttt tagagtctag 105960

atgagctttt acgcaatgtt gtacagtgtt gtatatatgt cttgtaagca tttgttgtag 106020

agtaataagt aaaagataaa taaaaatgac tattaaaata aagcccaaac cattaaaaat 106080

atttttatct gttagattta atttaataaa tggctcatgg aatgtgtggt gcgccgctgc 106140

atgaggtgtg gccgcatggg atgtggtcgc ataagatgta gctacatggg atgtggcatt 106200

tgcttgcatg taaggatcat gatgtgttgg gtcttcatcc cagcaataat cgccatcttt 106260

atctagctga attgtatacc ccattatata tcacttatta ttttttttta atgtttcatg 106320

aatttcatta taggcggtga aagggtcctc aggccccttc tgtaaaagat tatagagatc 106380

ttcggacgct ttatgtttcg tgcgaattaa ggcgggatat aacaaaagag agggccccag 106440

ttccaaacaa attttactta gcgggctcat attttgcacc aagtttccca ctacttgcga 106500

tgtttcataa cgcattttaa agagctttat cataaaagtg ttatgcaggc cggtgtagtc 106560

tggcctatag ttaaggaagg ggatttctct ggtaccgtca aacacgatct caagtcctct 106620

agcaagcccg atcaaaattt cttcagcaat ggatgagtat ctaattccta cattacgaag 106680

cgtaagcatt tctataacat catctatttc ctgcatagag gaatctattg taggaatttt 106740

aatatcatct gtgctgattt gttcattccc aagataggta agcagcatat taattttttc 106800

tagctttact agcttagtct tacgctcata atcatgatct tttttataaa aagagttggg 106860

atcaccgttg gaccgtagat gattaataag gcggtctact tgctttgtac taggtttaat 106920

acttttttca ctatactcgc tttcagcata gtggttttta cgatctcttt tagaaatagc 106980

tgttttttga gatgcctcag actctgcata tttttttcta tgcgtagaaa gagaataacc 107040

gcggtcatta cgtgaactac tgttgcatgc aaggcctcgg cgcgtcttac cgctgcgcac 107100

actgccattg cgtatactgc catcgcgcac actgccgctg cgtatactgc cattgcgtat 107160

actgccgctg cgtatgctgc cgctgcgtat gctgccgcta catacactat cactacatat 107220

gctgtcagta catacgctat cgcggcgtat gccgccgtgt accttatcgc cgcccctacc 107280

cgagggtttt ttagatataa tactgtgtgg ggagtcaagc gaaaattcag ggtcattaaa 107340

gttaatgccc aatgactttg ccaatccatt aagctcttca tcaaaatgat cggtaggaaa 107400

actttgttgc ttgcccatga cctgtttttc aagttcctcc aaattggctt gctcatttat 107460

atggagatta ttcataagcg tcgtaattcc agcaagattt gctccttcta aaaatgtggt 107520

gtcctccatc ggatatacta tactatttaa aagcttttaa ataaaaatgt gtttggaaga 107580

aatgctctct tcaagcgtgt gtagctcaga tataaatgcc tcctcagaaa gctttccacc 107640

atactccttt ctcatcgtat aggagggcgc cggtttaatg taggaaatcc actgggaggt 107700

aaaaaaccgg tacaacatat ttagcagctc gcgggcctcc caccttttgg gctccgtata 107760

gtgcacatca acataagagg cggcgcatga aaagctgcaa aagttgccga gaacgcccat 107820

ctcaatctct cctcgctcat tttcacgcat ataggtgggc acgaattttg ggacagtctt 107880

gaaatagaga tgacatgtcc agcatttaaa gctagaatgg gtaacccatt tggaaacagt 107940

ggtgaatacg gagggtagct ttttttcgac ctcggcttca tcgtcattcg tatttaacgt 108000

atcggtggca gtttttttgg attgcaagca ttcttcaatg gtaatcccgg ataagtataa 108060

aatattagga caattagttt ccataatttt gatagttatt tttatacaac atggatttaa 108120

ttaaagataa atggaggacg aaacggaact gtgttttcgg tcaaacaagg tgacgaggct 108180

tgaaatgttt gtctgcacat acgggggaaa aattaccagc cttgcatgtt cgcatatgga 108240

gttaattaaa atgttgcaaa ttgctgagcc ggtgaaggca ttgaactgca actttggcca 108300

ccagtgccta ccgggctacg aatctttaat aaagactccg aaaaaaacta aaaacatgtt 108360

gcgccgtccg cgcaaaacag aaggcgatgg gacttgcttc aatagtgcca ttgaagcctc 108420

cattttgttt aaggacaaga tgtataaatt aaaatgtttt cctagtaccg gggaaattca 108480

ggtcccgggc gtcatttttc cggattttga agacggaaaa aacattatac agcagtgggt 108540

agacttcttg caacatcaac ccattgaaaa aaaaatccag attattgaat ttaaaacgat 108600

tatgattaat tttaagtttc aaataaaccc agtgtctccc cgcgtcatca ttcatttaaa 108660

aaaatttgca gctttgttgg aacacatccc tactccatat cccatacgtg aaataaagcc 108720

tccattagaa gactcaaaag tatccgcaaa atttatggtc agtccgggaa aaaaagtacg 108780

cattaatgtt tttcttaaag gtaagataaa tattttaggc tgcaacacaa aggaatccgc 108840

ggagaccatt tatacgtttt tgaaagatct tatcagcgta cattggcaag aaattttgtg 108900

cgtgttaccg gtacccgatt aaagaatgtt ttcattaata aggtaatcga ctatgctaaa 108960

aagaataaca agaaaaatac cttgaagaac tataccaaag taggtaggtt ttctgcatgt 109020

cacggcatgg ttaaaattgc taataatgta gtccacaaaa gcattgctca atacgactaa 109080

aaatagtaaa aaaaggataa gtgctctttt tatatccata tactttaaaa cttatttttt 109140

acactaataa tttcctgcgg ccgcaatata aactgtaggt catctataac gcccagacct 109200

gttaaaagta gagtactatg ttttaaggga tttaaaatat ccgccgcaag aatgtgaata 109260

taattttcaa agtggtttac aggaatgcgt aagcgttttt ttttgcactg cggttggttt 109320

agggtcgaat actggcagga ggtatatata ttaataagac cgcggtcgat ggtttcaata 109380

tcttcataga attcaatgcg cggcgtcaaa agttttttaa gatgttgaca taactcatca 109440

tacgtgtagg actggagggg ggaaagaagg gtgtagtcaa agttaaaaat gtttttttga 109500

agaaccttta aagcatgttc cgcgtccgtg gtttccaaaa tatgttttat ggtatgaatg 109560

tcatttaaat ctacaaagtc tgacagcttt gtgtagaact cggtgacgga ggttattttc 109620

tggaaatcgg ttttttgaaa aagattttca atgtgtttgc gggttgagtt gctttgcagt 109680

ccatacaaga catcaaaaaa ttcaatcagc aaaaacttat acaaatggtt aatataaaaa 109740

gctttgttgg ccttattctg ctgaggatat ggttcctcta ggggatatag aatggcttgg 109800

tctatatccc taggatcaat agtcaatgtt gcgatgggaa gcttttccag cgtagcggga 109860

agagtttggg ttggagcgta gtaaaagtat agcccggttt ttccctctga aagaaagccc 109920

acaaattctt tttttatatt ttgcagcacc gctgagggta cgatttcgta ctgtttatac 109980

tgtttgttga aaagggtaat aaatttccag gtttcttcaa agcttgcaat ctgggtgggc 110040

cgcagatcaa agtcgatggg aatgtcgtca tgaatgtagg atgatagtct tataggaaaa 110100

taaatagggc gatcggtgtc tgaatcgata agtaaagcat aacaaaagtt atgcctgttg 110160

ataagttttt taccaaccgt gtagccggga atgtttttca cgtcatggat atcccaccag 110220

ttatccttgc acataaactc gctcatagac tggatgacct ccatcacagg gtcatcttcg 110280

gtaaaaatat actgggcctc actgtttttc agaaatcttt tttgctgggt gatggccatt 110340

gggtagatcc cttcgtccgt gtcaaagata atggctatct tcttcgatgg gctaagaatt 110400

ttttgtattg tgctggggga cacctcaaac ccgatgtcgc cctgtttatc tttaaaaaag 110460

acacagtgaa ggtcgtagca tatggcaaca aggtccagaa agatgtcctg ccatgtggtg 110520

tcccattgaa gcagttggtt tttttgttca acaaaggttt gtaagataag gtttgccagc 110580

tccgcgccgc tggaaaacat gttgccggcc ccattcccca aaatatagta ctgcggtgtg 110640

ttggccgcct ttgcaatttc aatggcaagg gccttggggg caagatccaa aattcgagca 110700

agggaataaa aaagcccggc attgctaatt ccaagcatgg tttgctccac ccccacaatg 110760

caaaaaatgt cgggctcttt tatcgtattt aaaaacagtt catctgctat ctggtggggt 110820

agaaaggcaa tccggttcac cggtattttt tttccatagg acaaggtatg acgcgatgtt 110880

tgtgtattaa gatcctccag gtcttgttct acaaacgtgt gcttggtgag gcaggtattg 110940

ttaatataga accgctttgt gcccagcagg gccttcgtct tttggcagca cggcagacag 111000

taatttaggg ggtggcggcc ttctagtagg cttagatgag ggtagtcagg atgcgggcag 111060

ctatagtagg caggtacccc ctccgtgaaa ttccaatact ttactagctc cttgcgcttg 111120

gctggcggca tggacttcac ctcggcctct gagtaaatga cgggtggccg tgggtgctgg 111180

cataggacgg agtaaaccgt tgcctgcgtg tcgtacttgc gcaggtcata caggtcgggg 111240

tcctgttctt gaagcgcacg tagctgagag gctccctttc cttgttgttt atcgtgcagt 111300

tgagagagtt tattaaccaa aattttgtca ggcccggtga tcaagttatc taaaaacaca 111360

aataggtaaa cccaaagata gttaaactct tcctgggtaa tgttaaacat ttctattttg 111420

atatctgtaa ccctatggta gatgcgaatg ttgcggccgc cgtagattgt ttcccaccgg 111480

gccgcaacat ttgtgtcaaa gaggtacgca tacgtgtttt ggagcaacgc aacattgatg 111540

tccattttgc gccccggacc ggaggaaata atgatcatcc gttcgatttc gtggggatca 111600

tacgaataaa tccccttttt aaataaaaaa ttgtagaccc cggtttgctg gaggccccgc 111660

acggaaataa tccctgcttg ctcgtattcc cgccaacgac ttttgagctc ggtaaatccc 111720

ttgctagaaa gcgtataggg ccaaaaggtg gacaccgaca tggagctgat agaaatttgg 111780

atgtcctcgt tggagggaag gggcagactc cctccacgag gaaacgcggc aggccccata 111840

tcattaattg tatgaataat aggatttatg aaattattta gggtggacac cacggagtta 111900

aagtcgtggc gctcgttttc tgaccaattg ctttcgataa agtagtgccc attattttgt 111960

atggtaagaa taaaggcctt tttattgata aagcgtatta aaataatagt gggtacacgg 112020

aatgttttat tgctgaattt ttcaggctcc gtggaagtta tgtggtgttt ggaaaccacg 112080

gtgggacctg ttttactata aaagaacacc accagctgag gaatatcggg agtagctgga 112140

aataggtcga aaacattgcg cacattaatt tgaatattta cgaggggtga aattttaatc 112200

attgccgagg tgacggccaa cgtgccgcgt gttagtctat tcccctcgta cttggcaatg 112260

acttgttgtg ctctggcata cgtaaagttt attagttttt gctctaggag aagcctcttt 112320

ttaagactgg tcaaggatgg agaaagagca ggatactgtt tttccatttg taagggagat 112380

tgtaccaata gtttaaaggc atcgggggaa agaagaggcc aatacttcat aataaggccg 112440

taatagagta agtcaaattg gtaattatcc tctatggcaa tggagatttg gcgccgcatg 112500

ggggccacta gcgtgttgag gtctgctaca aagatgtgat gaatgttttt tatgagctgg 112560

aagctgtcga gcgcttccac atagagctca tctttttgac tttccataga tgcgtcgatg 112620

ttcaccccac ccacctgttg aaactccttt ttgtagtcgc gaatgtctaa cgccaccccg 112680

ctaccgctta acaataggcg atacgttacc tgaagcgcat tgttttgaaa aaagaaaatg 112740

tgttgtctat aaggggggat ccctgtggca acgtaaattt tttctcgaat gtctttaaaa 112800

gtgtcttcag ggaaaatact atactcgcta tacatcgtct caatttctgg catcatcacg 112860

tttgtctcct cgccacgatc ctccacaaaa agtttttcaa actcatctaa atcatcgcta 112920

tctccaccca ccacgtattg ggaaagcttt ttctcccaat cctcgccgta aaaattttgt 112980

aaaatttctt tgtccttagg ggttcgctgc aggtctttgc ggcaggcctg taacacgttt 113040

gcaggaacgg atcccaaaaa aataaacgtc ttcgtgtact cattttccac aggattataa 113100

agagtaactc gtagaggatt tgttaaaaag tcattttgga aatccattat acccggtata 113160

gaaaataaaa tttaaaataa aaaacggatg atatctatca tggaccgttc tgagattgtt 113220

gcacgggaga acccggtgat tacccaacga gttacaaatc tcctacaaac caatgctcct 113280

ctactattca tgcccattga tatccatgaa gtacgatatg gagcctacac acttttcatg 113340

tatggttccc tcgaaaacgg ttacaaagca gaagtaagga ttgaaaacat cccagttttc 113400

tttgacgtac agattgagtt caatgataca aaccagcttt ttttaaagtc gctactgacg 113460

gctgaaaata ttgtgtatga acggctggag acgctcaccc agcgtcctgt aatggggtac 113520

cgcgagaagg aaaaagagtt tgcaccatac attcgaatat tttttaaaag cctgtatgag 113580

cgacgaaaag ccattactta cttaaataat atgggctaca acacggccgc ggacgacaca 113640

acctgttatt accgaatggt ttcccgagaa ttaaaactac ctcttacaag ttggatacag 113700

cttcagcact attcctacga gcctcgcggc ttggtacaca ggttttccgt aacccccgag 113760

gatcttgttt cctatcagaa tgatggcccc acagaccaca gcatcgttat ggcctacgat 113820

atagagacct atagccctgt taagggaacc gttccggacc caaatcaggc aaacgacgtg 113880

gtgttcatga tatgcatgcg cattttttgg attcactcca cagagcctct agcgagcacg 113940

tgcatcacca tggcaccctg caaaaagtcc tcagagtgga ccaccattct atgctcctct 114000

gaaaaaaatt tgttgttaag ctttgctgaa cagtttagcc gctgggctcc tgatatatgc 114060

acagggttca atgattctcg gtacgactgg ccctttatcg ttgaaaaatc tatgcagcac 114120

ggtattctag aagaaatctt taacaaaatg agccttttct ggcaccaaaa gctggatacc 114180

attctaaaat gctattacgt aaaggaaaag agagtcaaaa tctcggccga aaaatcgatc 114240

atttcctcct ttttgcatac ccctggatgc ctacccattg atgtccgcaa catgtgtatg 114300

cagctttacc ctaaagccga aaaaacaagc ttgaaagcgt ttttagaaaa ttgtgggtta 114360

gattcgaagg tagacctgcc gtaccatctc atgtggaagt attatgaaac acgagacagc 114420

gaaaaaatag ccgacgtggc ctattactgc attatagatg cccagcgctg tcaggacctt 114480

ctggtgcgcc acaatgttat ccccgatcgc agagaggtag gaattctgtc atacacctcg 114540

ctgtatgact gtatctacta cgcgggagga cacaaggtat gcaatatgct cattgcctat 114600

gccatccatg atgaatacgg ccgtattgct tgcagtacca ttgcccgagg taagcgggaa 114660

cacggaaaat atcccggcgc ctttgtgata gaccccgtta aagggcttga acaggataaa 114720

cccaccacag gtctcgactt tgcgtcgctg tacccctcac tcatcatggc ctacaacttt 114780

tcgccagaaa aatttgtagc ctctcgggat gaggcaaata gcctcatggc caagggtgaa 114840

tctcttcact acgtctcctt tcactttaac aatcgtctcg tggaaggatg gtttgtgcgg 114900

cataataacg ttcctgataa aatgggattg tacccaaaag tactcatcga tctacttaac 114960

aaacggaccg cccttaaaca agagcttaaa aaactaggtg agaaaaaaga atgtatccat 115020

gaatcccatc ctgggtttaa ggaactacag tttcgccatg ccatggtaga cgcgaagcaa 115080

aaggcgttga aaattttcat gaacacgttt tacggcgagg caggtaacaa tttgtcgccc 115140

ttctttctgc ttcctctagc cggaggagtc accagttcgg gtcaatataa tcttaaactt 115200

gtctataact ttgttatcaa taaaggttac ggcatcaagt acggtgacac cgactcatta 115260

tacattacat gcccagatag tctttataca gaggtaacag acgcatattt aaacagccaa 115320

aaaacgataa aacattatga gcaactctgc cacgaaaaag tgcttctgtc tatgaaagcc 115380

atgtctacac tatgcgccga ggtgaatgaa tacctgcgac aagataatgg caccagttac 115440

ctacgtatgg cctacgagga agtactcttt cctgtgtgct ttacaggcaa gaaaaagtat 115500

tatggtattg ctcatgtaaa cacacccaat tttaatacaa aagaattatt catccgcgga 115560

atagatatca ttaagcaggg tcaaacaaaa ctcaccaaaa cgataggaac gcgaattatg 115620

gaagaatcca tgaaactacg ccgccctgag gaccatcgcc cccctcttat tgaaatcgtt 115680

aaaacggttt tgaaggatgc tgtggttaac atgaagcagt ggaattttga agacttcatc 115740

caaacagatg cgtggagacc ggacaaagac aacaaagcag tccaaatctt tatgtctcgc 115800

atgcacgctc ggcgtgagca actaaaaaaa cacggcgctg cagcatcgca atttgctgag 115860

cccgagccgg gagaacgctt ctcctacgtt atcgtggaaa aacaggtaca gtttgatatc 115920

cagggccacc gcacagattc ctccagaaag ggggacaaga tggaatacgt ctctgaagca 115980

aaggctaaaa atcttcctat tgatatattg ttttatatca acaactatgt tctaggcttg 116040

tgcgcgagat tcattaatga aaatgaagaa tttcaacccc ctgacaacgt cagcaataag 116100

gatgaatacg ctcagcgccg agctaaatcc tacctacaaa aattcgtgca atccattcac 116160

cctaaagaca agtctgtcat taagcaaggc aatgttcatc gacagtgcta caaatacatt 116220

caccaagaaa ttaaaaaaaa aataggcatc tttgccgacc tttataagga attttttaac 116280

aacaccacaa accccatcga aagctttatt caaagcactc agtttatgat acaatacttt 116340

gatggagaac aaaaagtaaa ccattctatg aaaaaaatgg ttgaacagca tgctacggct 116400

agtaatcgag ctggtaagcc cgctggtaat ccagccggca atgcgctgat gcgggctata 116460

tttacgcagc tgattacgga agaaaaaaaa attgtacaag ccttatacaa taagggggat 116520

gcaatacacg atcttctcac ctatatcatt aacaatataa attacaaaat tgccacgttt 116580

cagacgaaac agatgttgac gttcgagttt tccagtactc atgtagaact gctattaaag 116640

ctgaataaaa cgtggcttat tttggctgga attcatgtgg caaaaaaaca tctgcaagct 116700

tttttggatt catataacaa tgaatcgccg tctagaacat tcattcagca ggctatagag 116760

gaagaatgtg gcagtattaa accatcttgc tacgacttta tttcctaata cttcttaaga 116820

aactctttaa acaaggactt cgcatggtca aaggttctaa acccatggcc cttatgattc 116880

gccaaaaaag cggtttcatc aagattttct aaccctttca cggatgaaga aataaggtgt 116940

tcggcctcgt ttgcccattt tctatgattt tttttcacct cgggttctag atctgttttc 117000

tccatatact cattgtggtc atattttttt ttgggaggag gcgtgggtgg aggaatgggt 117060

ggaggaagta cacccgactt tcccgcttca accgttttat aaaaaaatag aagcataata 117120

caaagaataa ggactatcgc aaatatgata accagtgtcc cagtcgaggg cattttgtta 117180

tataagtaac gttttttttt attttttata attcgaatga agaaccatgt tgaatagtct 117240

tctactcaaa gacattttgt tatacggtaa atgagaattt ataaaatccg aatatcacta 117300

tcatactgtt tatctgagaa ggtctcactg ggtcctgtga tggagaaccc atactctgta 117360

atgctggggt ttataatgtg gtcaggactg acaagcacat ttctgaactg cgagagttct 117420

aggtttagac gcagtcgtaa tagtcgctgt atatttgtaa taaatattag attgcgtatg 117480

aggcgagtgt caaagcgatc ctttccaatt tgtactaagg tgggcttttg tattccaact 117540

cccacttgtt taacgatgga ccagggtcct tcttcccgat tttgttccgt gatataggtc 117600

agcacactat tttctgtata tgaggtatga tgtcgcatat taatacctgg tgccattcca 117660

actggcggtt gtgcaattcg ggctgtaccg ggacccaacc atcgtggagt tttataaaca 117720

tatcgttcta gcgtatttaa aaattcctta aggttattta cgagtagcat gaagggtgct 117780

attaaaacag gtggatggtt tataaccatt gtcataaacc attgcattgc ttcaatatca 117840

ttttgtaatg cttgacgggg aggcggggca ggtaatccac gtatgttgaa taaagcggtt 117900

aattgtgcac cggctgtttg gggcgtaata ttttgtatta aatttatcat cgaattggct 117960

tgcccggcat ttcctataag atcgattaaa ttggttattt gacctcgata ttgttgtacc 118020

cagttttgaa tggcagcgat gatctcaggg gttggattgt tttgaatttc aggtgtttgt 118080

attagattat tcacttctct tcgtgtatct tcaagctgag tcctaaatgc atttaactca 118140

cctataattt ggtttctatc aataacattt cttaaacctc gaactgtttc agccaatcgt 118200

atagtacgca caatttcatg taaggcctgg tttatgtata ttgacatggg atggccccac 118260

cgctcacgtc cacgttgaat acctgcggcc aaactaggac ctgcctcgtc ataatcaaat 118320

tgtgtaggat aaaggcttcc aaatagcact ttattgaaaa tttggtcaga aagaaattta 118380

gggcggccca tatttagcgc gttgtcccct ctaaagatgc gtgacatgta tccggcgttg 118440

cctttggata gtaactcatt cccatattga gtaatagaga ccgagacata ggggtttata 118500

agaagtttta gcataaattc tcgagtattt atggggggac gattcggaat gtttaatacc 118560

tctgcaacat ctggttgagg agccgtggtg tccagagatc gtactttttc agccgaaatg 118620

ccgtacataa gacaagcaat ttcttcaaaa ctatagtcat agttgtaaat attggcaagt 118680

ggtatagatc gcatcagcgc atttacattg ataggtataa tattcatatc aaacaagtta 118740

aatatgcgct cgcgctctct attagagcca agagtgcgtg tttgaccttt cggcgacact 118800

attttgtgaa tatgattgat ttgctcctct tggtaagagc tttccacgaa ggaaattacg 118860

tcttgcaatg ttttacgaag cgaatacact gcattcatcc ctattcccgc tgttataatg 118920

ggtttatcgt ctctgttctc gctaataaga ttaactccac caaaagtatt ttcattgtac 118980

atcatcactg ttttaaaact acggatattt atgataaatc ggagagcctg aatggcgtgg 119040

gtataaaagt gttcaaatcg cgtgggagta atttgttcgc gagcaactac cgtttcatta 119100

tagtttttca tgataagctg tactccgggc atatctgaga gctgtaccgg atcatttccc 119160

agtaattttc ttgtgccgta tagtagttta aactcggggg agccgctttc aaggttcggg 119220

taaagaagag gatcatatac ctcattattt tctattctta ggtcatgtaa ataatagagc 119280

gaaagtgaaa atggcataag aggctcctta ttgtaccggg acatatagtt ttgaatgaag 119340

tgttcttctg tttcaagata gatgggatga tcggtaagct cgtgcaggac ctccatggca 119400

gaatctgcca gagtgtgaga gcctctaatg atcccgtcga tcactgcgac cagtcgcttt 119460

cgcacaacat cgctcgtatt attttgtgcg tctcctaggg gcataagcgt aacattggga 119520

cgaaatacgc cgccaattcc ccgcagggcc gcctgaccga cggatagtcc tgtcgcagga 119580

acattgttat tattataata aataacggaa tcattattgg ctcccaagag tgccgtcaga 119640

ttagggcgag ctagttggac atttgtgtat tgtataaatt gttttagaag ctctccctgg 119700

ctaataagaa tattaaacat tttgttaaat agtggaagat tggctctata attttcttta 119760

aggtaaatgg gaatttctgt taaagtagaa ataagatgct gactcaggcc ctggcgattg 119820

gtatccttaa taagccgctg aagtataagt cccaaagaca gaagaagcac cgactgctct 119880

gtggggtcgc ctctatgacc aaagacgttg ttattgcgtg ctaagtcagg gtgagcatat 119940

cccatctcca tcactgcttg gctaaagttc ccattagcga atgcattaat aagatttaga 120000

tatatttttc cgctgggagc atcataaaat cgggtaatat atgaagctat gagctggtta 120060

aacaccatca tcatactacg attattttga ataccatagt ctgatccgta taggcgataa 120120

cgtcgaaggt tgtttgcggc atcattgaca ttggcatagg ttctgagcgc tatgttgtcc 120180

cagtagctaa gagtattttc ctcctgggcg ttgttggtac gaataagatt ggagagtcta 120240

aagtctccta gtgccacctg ctctacacga agtccagagt tattctccaa agcatcgtaa 120300

aatacgagtc tactgaatac tcttccgtat tgttcaaagc gttcagagga ttggggattg 120360

ttatttattt gaatattagc cgcgtccctt ctttgcgccc cacctcgaag ttgcagtaca 120420

ttataaggct ttgtaagcaa ggtgtaggtt ttattaatga tttggttaac cccctccagg 120480

cccaattcac cgccaggaag cggccttcct ccggcatcgg taggtggttt aataagtttg 120540

tcaattaaat gttcttccaa ccagtaaaat gagccaggat tagatctatt ttcatagtat 120600

tgaataatgt ttttatcaat atgcgggcgt agaagatcaa gaaaatactt cgtgtcggcc 120660

atcaaagaat caattaagga aataagacct gtaaaatcta aatgcacttg agcggtgctg 120720

gtttcaggga agcgaacttg aaccattttg ttaaaactgg aggtcatttc gaagatattg 120780

gtcaacagga gctgcatgat tcgctgatta tctactaaat accttgcggc caactcttgc 120840

tccggacgaa ctcctccacc agcaggaata cccacatatg gtacaatcca agcaaaaaga 120900

gtttctgtgg ttaaatttcg gtcttgggct gctgcagccg cttcggtagt gggatcaggg 120960

tacaccatag aaagccgcat attgatttct ttaatgacta atcctggatt tctaatctca 121020

gagatggccc cgtgttttct tccgagccag tcaataagat tggcgcggtt cacgttggca 121080

gcttgtgtct ctcgtaacca ttcgataatg cttttttgaa tcgtatctag gtctaaacct 121140

ttaatgttat tacgaaagtt attaagaagt acgtaaatag cactcaataa gttaagacct 121200

gtaataacgg tttcatgaaa cagaaatatt ttgttaacat ctgtatctgc cagtgactca 121260

gagccttgaa taagttttga aacgatttga attttatcgg tatgctcctt tttgagttca 121320

ttgatagcct ggcgaatgag ttcttggtag gaaattttgc ccaattcttg ttgcagactg 121380

ggatcttcaa acatctcact aagctgtttc ctaaattttt gtaccaaatc ccactgggag 121440

ttgggctgca gcattcctgt ttggacatcc acagagtcta tattgtatag tgccgggcgc 121500

cacttggggg taggctgggt tgaaggacta ataaacctat cggagggaag taattgtgag 121560

gattgtgtat agccatcctc atcaggaaga atggagtagt tggtttgatt catcattcca 121620

aaatcattca tagttcgcgc ttcctgaaca atgcgttgaa atttttccca ttcggtgcgt 121680

gtaatgacac cgaatctgcg gtttatttca tttacaaaat ggataagcgc ttttttggtt 121740

gcttcttgtt caccatactc taagttaaag tgttggtaaa tgacgtttat ttctttgata 121800

agctgacgaa tttcggtttc tgagtagtca ccaatgttaa taagctcaat aggacgcata 121860

aagataatgc gaataagtcc tgagaagatt ccttccagct caggaagcat cgagatctgt 121920

acattttcat ctctaaagga aaacaacttt tgataaaatt cggcgaggcg gggaaggcgg 121980

aagtaaagct ctgctgcctc gggaattacc tcgggctcta gctcatcggc accccccaat 122040

atcatacgcg tgggtataag tttgtacacg ggctcaggcc gttcaaacat gtcgtaaatc 122100

cctaatacaa taaaaatctt ggcggccata cttttcagca tgaaggtgaa gaagacgtcc 122160

tcggtttccc agcgggttga tagggcgtcg ttaactctca cagtagagag gtagacccgc 122220

tgagccgctt cctcggcagt ctgtgcaagc gccatccttt gtcctccaat ttctgattga 122280

tttagatttt taagtcccac ggaaagcgca gaatgttgaa gatattcaag caaggtttta 122340

tagatttgca ggggcgacat gggcaccatt tgccgcagct cctctccccc aagcatgtcc 122400

ccaatccggg caaaggcatt gatgatattt ttaagcgcct gaaagttaga aagagagcgc 122460

ccgataaggt cgcgaatgtt tttagcctgg cttgctctga cgggacggag ggtaccaacg 122520

cttcggcctt gttggatttc agccgcaact ttttcgtagt agtggcccgc aggagcatta 122580

tccgtaaaga cgttggagtc gttgcctgtg gaggtgggaa aactttcaaa gacttgtgca 122640

agcgtgtccc ctgttgtctc ggtgaaccat cgtcctataa tgcgcacgcc atccagcatc 122700

tgttggactg tttgaataga atctatgttg tttacaaacg ttttggtaat gtttttaaga 122760

taaagatcta gcccttccag agctcgatag aatcggcgtt ttacatcata ctccagctcg 122820

atggcgctta cggttgcctt ccagtctact tcctgggcac ctccaggatt tgggcccacg 122880

tgtcctctgg caagatctac agccggagaa ttaatgcgcg catttttttc cgtatccaac 122940

tgcatgaggc gtcccgcaat agcatctccg agaatagtgg catagttttc ctcgtaggat 123000

tgaaactcct gtttgttatg cgttaaattg gagtaaatct gggccacata atagtaatac 123060

ataaaggtgt taattgcctg gttgaggtca acctgcgatc gcgcggcctt gctgagccca 123120

agctcttcaa ctgttagggc agcaccgcct acccttgtac actcgcagtc ctcctcgcct 123180

ccatactttt tttgcacaat atcggtataa aaatcaataa tctgtagcaa gcgagagcag 123240

gagtcataaa gatttttaaa attagggtcg gttttagata tctcctccaa aacattttta 123300

acaagcgtaa gctgtgttaa gaaggtttcg cgttcttctc gtgcggccgc attggtgtaa 123360

aagccgataa gacttagatc aagtgcgatg gtgcccatat cattaatgcg cgaaagagca 123420

tctcgaagcc tcgttatgtt cggcgtcaag gcaatttctt taacaagttt gatgcctatt 123480

tttttcacat tttccaaaaa gtcgttatag gcttgtgtgc ttttattcaa aaattccatg 123540

aggatgtgct ttctatccag tctttgcgct tcaatcctcc tatctagtgg cgttttctcc 123600

tcatcgcccc cctttttggc acaactgttc tcaaggattt tgtggcgttc attaaaggtc 123660

tgtcgcaaca ggttcacggc tttttcaaac tcagcaatgt tttctgcgga gacaagacca 123720

ctaaaccttt tgaggtcaag ctccttgtca aactccgccc agtttttgct ttgaaggtac 123780

tgttcaacct tgagtcctac tttctggaga gccttattaa ttttattcgc aacagacgca 123840

gcaataccta gattacaaag tgtgtacgaa agtacttttc caaaattttt ggttcccaag 123900

acactatttg tatcatttaa aagtttaata atatccacct catccgtctg cagtttatca 123960

agttcctttt gggtgggagt taaaatattg tcaataaaat tcgttaaaat gttgatttgc 124020

aggttttgtt catttaaaag tcgacgatat actgcttcaa tcatggtgac tgcattaatg 124080

acttcctcat tgggggctgc tttggttacc tccgtcacca tgcgctcgtg aagttgctta 124140

atggcgtcgt ttaacagctt gatattttca agtgtatttt ctatactgcc gtgtacatca 124200

agatactctg cgcgcagtcc atgagttagg gagttaatgt acagaactat ttgtcgacat 124260

atactggcgg ccccttcggt ggtatctata agcttatcct gacctaaatc aataaattcc 124320

tggttaatgg cgtctgcaat cattttacag acggtctcct gtttttccgc attttttaca 124380

aaggtggaac cggctcgagg atcgggcagt tgttttttga tatctttaag aatatcttcg 124440

atgggctgct ttgtgtctac tttgaaccct attttggcaa tcgccctgat aattccttct 124500

ataatccgca gctttgcttt actcgatacg gagtctatgt gataatcttt aatgtgttgt 124560

acaggatttt tgtccccccc gccattaaaa tatcctcccc ctgaaaaagg acgagtttgt 124620

ctttgtatat gatcctgtaa cttcgcatat atatttgctt ctgatgaagg cagtggtcta 124680

ctagaggttg aagatccacg gttacccatt ataataaaaa aaaataaaga tttaaaacta 124740

caaatatttt gctgtttata aacccaatca tataagacta actaaaacat taaatgtagg 124800

tgagataaaa gcttattttt tttttaaaag tttaataacc atgagtctta ccacctcttt 124860

ttcttcttcc tttagagggg ttccataaat ggtttgaata aaattatgtg ctctaataac 124920

cttgttaaaa tcaggtgcct ttccatattg ttcaatatgt tgcacagtct tttgtgcaag 124980

catatacagc ttggagtctt taggtacctc cgatgagggc tcttgctcaa acaacgtttc 125040

aaaggaggat gtgcattcat tggtttcatt atcatttttt tcatgaatgt tctccgaaga 125100

tgctgaggat tccgtctcct cttcaaacag cacatgcaga atcatattcc attcttcttg 125160

agcctgatgt tcagtatacc cttgccctgc atatatacga gcagatttca caatatcata 125220

cttaacagta ctaagcaatg tttttatagc ggtcgtaaca attctaccgc tattgataat 125280

ctcaacagaa aaccaattat acaggctacc cgcatgaaac acaacttgtg aagatgatct 125340

taaatccgtt ttgaagatga cctccatttt catggatata tttaaaataa aatccattca 125400

attttaaaat tataaaataa taagaagatg ccctctaata tgaaacagtt ttgcaagatt 125460

tctgtatggc tacagcagca cgatccagat ttattagaaa ttatcaacaa cttatgtatg 125520

cttggcaatt tatccgcggc aaagtacaaa cacggagtta ccttcattta ccccaaacag 125580

gcaaagatcc gcgatgaaat aaaaaaacat gcctactcca atgacccttc acaagccata 125640

aagaccttag aatcactcat ccttccattt tacattccca ctccagcgga gttcaccggg 125700

gaaatcggct cctacaccgg agtgaaatta gaggttgaaa aaacggaggc gaataaagtt 125760

attttaaaaa atggagaagc ggtcctagta ccggcggccg attttaagcc ctttcctgat 125820

cgccgactag cggtctggat catggagtca ggctctatgc ccctggaggg tcccccctat 125880

aagcggaaaa aggagggtgg ggggaatgac ccgccggttc ctaagcatat ctcgccgtat 125940

actccgcgca cgcgtattgc cattgaggtg gaaaaggcct ttgatgactg tatgcgtcaa 126000

aactggtgta gtgtcaataa tccctatctt gccaagtcgg tctccttgct gtctttcttg 126060

tcgctcaacc atcccaccga gtttattaag gtactgccgc ttatagactt tgaccccttg 126120

gtgacctttt atctacttct tgagccctat aaaacgcatg gggatgactt tttaattccg 126180

gaaaccattt tattcggccc taccggatgg aatggtacag atctgtatca aagtgccatg 126240

ctggagttta aaaagttttt tacccagatt actcgccaaa cctttatgga catagccgat 126300

tcggctacta aggaggtaga tgttcccata tgttactcgg atcccgaaac cgtacattcc 126360

tatgccaatc acgtgcgtac tgaaattttg catcacaatg ccgtcaataa ggttacaaca 126420

cctaacctcg tcgtgcaggc ctataatgag ctcgagcaaa ccaataccat acgacattac 126480

ggccctattt tcccggaaag taccatcaac gcactgcgtt tttggaaaaa gctgtggcag 126540

gatgaacagc gatttgttat ccacggcctg caccgcacgt tgatggatca acccacctat 126600

gaaacctctg agtttgcaga gatcgttaga aatttacggt tttcgcgtcc cggcaataac 126660

tatataaacg agcttaatat tacaagtccc gctatgtacg gcgacaagca taccaccgga 126720

gatattgcgc ccaatgatag atttgccatg ttggtggcct ttatcaacag tactgacttt 126780

ttatacaccg cgattcccga ggaaaaggta ggggggaatg aaacccaaac cagtagcctt 126840

acagacctag ttccaacacg gctacactct tttttaaatc ataatctaag caaacttaaa 126900

atcttaaacc gcgcgcagca aacggttaga aatattcttt caaatgattg tcttaatcaa 126960

ctgaaacatt atgttaaaca cacgggaaaa aatgaaatac taaagttact tcaagaataa 127020

gtatgttgat acctgtggtg tgttttacct gtgggtttcc tattggaacc tacgcggcaa 127080

tttttgacaa ggctcgtacc gagtatatta aaaccaaaat gggcggaaca ttgccgcaaa 127140

atatcccatt agatgcttct ctccagattg agttaaaaga cctcattaca gctctgggaa 127200

tcccaatgcg ggtgtgttgt cgcactcatt taattactac gttggattat cgtaaatatt 127260

attaatatct aaaattgaaa aaatattttt aatgttacta gtaaaaatga ctacacacat 127320

ctttcacgca gatgatctcc tacaagcatt gcaacaagca aaagcagaaa aaaatttttc 127380

atctgtattt tctttagatt gggataaatt acgcacagcg aagcgtaata caacggttaa 127440

atatgttacg gtcaatgtca tagtaaaagg caaaaaagct ccgctaatgt ttaactttca 127500

aaatgaaaaa catgtaggaa ccattcctcc cagtaccgat gaagaggtta tacggatgaa 127560

tgctgaaaat ccaaagtttt tggtgaaaaa acgtgacagg gatccctgtt tgcagttcaa 127620

caaatacaaa atctcgccgc cattggaaga tgatggtctc actgttaaaa agaatgagca 127680

gggtgaagaa atataccccg gcgacgaaga aaaatctaag ttgtttcaaa ttattgaact 127740

gttagaagaa gcctttgaag acgctgtgca aaaaggtcct gaagccatga aaacgaaaca 127800

tgttataaaa ttaattcaaa gaaaaatttc taatagcgcg gttaaaaacg cagacaaacc 127860

tttgccgaat cctatcgcac gcattcgtat taaaatcaat cccgctacaa gtatactaac 127920

accaatattg cttgataaaa ataagcccat tactttacag aatggtaaaa caagctttga 127980

agagttaaaa gatgaagacg gcgttaaggc caatccggat aatattcata agcttataga 128040

atcgcattct atacatgatg gcatcattaa tgctagatct atttgcatca gcaatatggg 128100

catttcattt ccgctttgct tggaaatggg agttgtaaaa gtttttgaaa aaaataatgg 128160

gattgatgtg aactccattt atggctcaga cgatatttca actcttgtta atcagattgc 128220

tattgcttaa acaatttgct caaaacaagc ttataaacgt ttcttaggta tgcgatacgt 128280

aaatcctaat tctttaataa gttctttttc agtagtgatt tttagaggta ctaaagtttg 128340

atttttaaat aatccatact gatttagctt ataattcttt ttttttaacg cagctcgaat 128400

tcttattaaa taagaaacgg gacccgtaaa atgaagtact gcgtatggct tttcctcggc 128460

taaggccgta aaaagatcaa gttgatatgt gttttttttc cattcaataa aaagtacaca 128520

ctttcgttct ccgcagactt ttacagaaaa agaaagatcc tttatgcgaa tgttgggcag 128580

gacgtgtttt aaaagttttt tttctggaac aataataaga agatccacgt cattaagcat 128640

tttctcttcg cgtcttaagc taccaacagc aacgatgttt tttgataaaa tttttataag 128700

ttgtccatta tattcaaacg caagtcggga gcgtaagtca tttacaattt tttttccttg 128760

aataagcgtt aacattttat atttaatatt aaaatctttt cattttatat attatatacg 128820

caaaatggca cttgatggtt caagtggtgg aggctctaat gtagaaacat tacttatagt 128880

agcaatcatt gtggttatta tggcaatcat gctttactat ttttggtgga tgccccgcca 128940

gcaaaaaaaa tgtagcaagg ctgaagaatg cacatgtaat aacggaagct gttccctaaa 129000

aacaagttaa aacatgcaat tatatgcatg catataaacg catgcatata aacgcataca 129060

tataaaatgc gtaaatacta tataaaaaac tataacatat caatcaagga atcaacactt 129120

ttataatttt ccgtaatata tttttcatcc ataatgatgt cagagtacat ggtccctatg 129180

cgaggaacag agcccataag ggtaggcgcg gcaataccgt aaatgggatt cacggcggag 129240

tcaaccgcag catctgtcaa gacctggact ggagacgaca aggccattcg caacaacacg 129300

ttggaaggct ctcttgcatt aagccctgcc ttttctagag aggtaacctg tcccgttctt 129360

gtcatgagat ctgcgtacat gagtaaatga cgatggttgg gacccttgtc ccccataacc 129420

gttctaattt cactaataat tttttgccgt gccgcttcta tgccgtaaag ctccatggtg 129480

tctcctatag aggacgatac gatggtgtat gggtcgatgt tatcatcaag cattgcgcca 129540

aaaatattag tcccgtttgt tttgatggcg tagatattgt ctagtcttac cagtttcccc 129600

tgggcatcca cacggtggcg cataagctta acaacattcg catttttgat gcctggtatt 129660

cctctaatcg tgctatttaa tagtttatcc accacattta cggcaatttt ttcatccgta 129720

gccattcggg tattggtact gcgtctaaag gcgctttccc gtaggtatat gcgaataatg 129780

atgggaatcc ctgaggccgt gttttccaca gaatgcatga tgtaggtgtt ggggtgttta 129840

gctcttagac tattaataat actttctaga ctaatgcttt ttaatatcat ggttgttttg 129900

tttaattcca agcggataca ccagtttgca atatcctctg ggggctgtag tagaggatgg 129960

ttttccagaa aatccgtcat ccattccaca tcacttgcaa aatcggggta catcacattt 130020

ttttttgtgc ttgaatacgt ttcgtacaat aggtgccact gcaatatcaa ccgttcgaac 130080

gttataagct ctatgctgtt agcaatttct tgcgcatatg ttttatttgt ttccacttcc 130140

gggttcttta gacgtaaaag catttcagag gattgttcag cctctacggg cttcgcgcta 130200

aagatctcct ggggccgcac aattcccgac ttgttggttc ccccggccac ggaccggtgg 130260

tgggagtcca gcatatattg tgtcaagggc tctgatacgg actgcgccgc caggattccc 130320

actgcctcac cgtagttaat aagactttga gtatattgta gccttatgag gtccaggatg 130380

gcactcatct gctcgcaggt aatgtttaat gttttaacgg ttgccagttc gatgcgaata 130440

agcatgcgca tcagagaggc agcccgttta agataaacgg gtatgggcgt ttgtagtcgt 130500

tcctgaatgt tgttaataaa cacgtatgga agatttttgc aaaacgtttt gaccatcgcg 130560

tatttttgta gaatactttt ttcgtcgaag ggaagcacgc cactggtgga gctcagtaga 130620

atgtttttta cgatgctggc cacgtttacc ggcacctgtc taacatctgt aagcagctga 130680

ctgaaattaa aattttcgac gtttaggaag atctgtcgat atttatctct atccttttta 130740

aggcgtgaaa attcttcttc aaacaagggc gattgtatcc cggtgtactt gaatttgtct 130800

tcaagttcct ggtccgacag catgatggtt tcaaaccgta cggtttcaag ctggcgcgca 130860

tcaaggccgt cctctccgta caactgctgc acaagacgcg tatcgatgga aacccgtcgg 130920

taataatcca caatacagga ttgaaggcca aagatggctt tacggttggc atagcctgtg 130980

gatgatgtcg ataatgcttt gttgatcaag tcgaatcttc cattcatttc cccaaagata 131040

aattcagggg aggtaaggcc cgcaatatag ctgttgcaga tgaacccgta ggcctgcgcc 131100

tccagggcaa acctggggta gtacaccagg gtcctaccga aggaaaactg gggttgaatg 131160

cgttgtgtat taatttcaat ttggccgatg cccgccatga tgtgaatcat attggggttt 131220

gagcccttgg cgccagtggc caccatctga aaaagcccat tggtttccgg attaatggaa 131280

ttcataatcg gctttaaaat tctatcggga aatttaagcg cattcagctg caatttttcg 131340

tagaagtcat gcgttgtcag gcctataggc ggcatgatgt ctccatgaag cagccggttg 131400

tttatttcct ccgactcaag cagcagttca ttgataattt cttggacctc ctgatgtgcc 131460

tccggggtta ggagcatgtc ggccgtggac actgtgaatc cggcgttgcg cacgtagttt 131520

agggcgagct gctgggtcgc aaatatcatt ttcaaggcct gctgcggccc atacctacgc 131580

gaaataaggt gatagattcc accggaggaa cccgctccga cggccttttt gtcaaggacg 131640

ccttcaatga gttcgccgtt gcgtatttgt gtagagatgt cctgcttgtt ataatgcatg 131700

tagggtgcat acacttctga gtaccatgtg ggggctcgtt gataattgat gggggtctgc 131760

ctcagtagca tagatacaac cgatttgcca tccagcaggt cagttgggga gtagttggca 131820

aaacaaggtg ggtcggtttg ggttgtttga aacaacccca tggcgtgcag cttgttcatc 131880

acatttttcc ccatgggggt gttcgtgcgt gtaagcaaaa agcttcccac cgtggagtcc 131940

tgcacctgcc cattaacggg acccgagctc tttgtggaaa tgaaccagtt tcgcacagaa 132000

caaagtagtt cggcctcaac gcggctcatg acgctccagg gaacccagag attcatctga 132060

tccccgtcaa agtccgcatt ataccaggca catgcgctga cattcatttg aaacgtagaa 132120

atttttgggt tttcaagaac gacaatccgg tgaaccccta tgctgcttcg ttcgagagaa 132180

ggctggcgat taaaaaacgc gacgtcgcca gtgacgacgt cacggtaaag gatgtctcct 132240

acctccagcc taaagtcttg tttgagaccc tcaatgtcgt gaacggattg tgttatttgc 132300

ttatacactc ttgaacaacc agggtactgg cgctttccat ttaaaaaata gggcattaat 132360

ctattaatat tataatgttg cactgtttcc gcaacttgca gcgttcgtgc aaaggaaatg 132420

ggatagccaa cctcgtccag gtgaaggtct gagttcccgc agatggtgga ccggctgatc 132480

gaccatacct ggctgcccag tagggattta cgaattcttc cctccttgcg aggaagtctt 132540

cgcatgatgg agggagcagg gcgtgccccc atgacgatcc cacgctttcc cgtgcctccc 132600

tgggttgcgg tggtggaaac ggaatccaac aaaaagttat agtaaagttg ctgtatggtt 132660

tgcaaattgc ggtcaatatt taaaggtatt ttttggccgc gcacgatttg taggtccttc 132720

gggatcagca gattctttcg aaccagatac tgaatcacgt tgttaatgtc gtgaaagctt 132780

tgggggcctg acccgattcc caatctgatg ccaggtcgta tgctgatggg ggggatctga 132840

atggccttaa gcacaagttt ttcgggatgg gagtttttac ttcgccccag ttttacaacg 132900

gtgtcgtagg ttacgcgcga aaaaatctct ctgatgatct gcgggtacag tttgtcaatc 132960

ttgccctgct gatccgccca aaaggtaaaa taatcttccg agtccttaac aattttgggg 133020

tgtactgcct tacagacgta gcactgcttt ccttcggttt ggcttgaagc cgcttcaata 133080

agacgcttag gcctaataag gtgctcgtac ctctttaggt caacgatggg agccccgcag 133140

ttgagacata taacccttaa ccatcgtcgt atttcggcga tgaagagcgg ctgaagcacc 133200

ggagcatgca tctgcagtat cccagggtgt cccatacatt gcttgcgctg gtgtgagcaa 133260

gtgatgcatt tataatggtg atcggtggtt cccattcgcg catcatagat acccccttcg 133320

gcgggaaggg tgccctcaaa taaattagaa atggtaacct ccataacgcc ttgcctctta 133380

tgatcattgt caccggcaat attgaactga acggcggcta tttcggcata tccagcctcc 133440

atatttttgc taaatacata ataaaacttc aaatgttaaa aaaaaataac atcggttggc 133500

atattttttt gttaaaacca agtgttaaat gatttctaaa acacttatcg gttcacgaaa 133560

acctaccgca cgggcctgaa gaggaatgcc agttttgggg gaaagctcgg catattccac 133620

ggtaagctct tttccataaa gatgtttttt aaataaggcg ggcgtgagtt tttgaaaaag 133680

agcataacga tccgcgtacg tcaaatgctt aggagtgact acaaaccgct ttttgtttgg 133740

caattcgcaa acccataaaa tggcgcctaa gtcctttccc ttttttccct gagtatagtc 133800

cactaaaata aattcagcgt ctagcagcgg tttcagcttg gcaagatgcg ctgagtggta 133860

gttgttgtat cccggctcat agggcccatt ggcattgcgt acgatggctc cctcgtagcc 133920

ctccttaata aactgcgcct taagcctaag ggcctcatcc acattcttca cgctaaaatt 133980

ttcaacttgg tggataaagg taagatcttc cttctgttta aaaatatttg ttaatagctg 134040

ttgtctcttg ttggaaggca tttgaagctg atcactccaa aaacagtcaa acacgtaaaa 134100

gtgcagctcg gaggaatctg tcttcgcatt cgcctgcccc gcgatccatt gcagaggttt 134160

gcggtgtaaa taaagctcac catccaaata tactctcacg tctataaata aataaagctg 134220

tttgagctct tttttaatat tgtcaagacc taaaaattcc tttttcgtgc gcgaatacaa 134280

gagaatgcta ccatcgccct gctggcaggc cacagctcga acgccattac gcttgcgctg 134340

cacgatggga tctgtttctt cttcaaaaaa tgtcttagga attatattaa aatattttac 134400

cagcataggg gggataattc ctctatttgt gtgggctccc cgcttttgtc tggcatggcg 134460

attatattta ctaagggcgt ccttgaatgc ctgatggact accgttgtgg catttttttt 134520

acccaagttt tttccctcgg taacacgtgt catttttgat atccgcaccg ccccttcttc 134580

cacaaaaaat tttgtgaaaa tttcagcaac ggcgtctttt acatctgtgg aaaacatctc 134640

atctgtgatg ggaatgatcg tgttgtgctg caccacttgc acacaaataa tccatgaggc 134700

cttttttccg cttttcgttt cagactcaat cggaggaaaa caaaaaatgt tgtttgaata 134760

ttgcccagga aattgattta gcatggtttt aacaataaaa taagcctatc aattttttta 134820

taatttgaat agttattcca aattcaatat ggcttcttta gataatttag tggcacgata 134880

tcagaggtgc tttaatgacc agtctcttaa aaatagtact attgaacttg aaatacgttt 134940

tcaacagata aattttttat tattcaaaac cgtatatgag gcacttgtgg cacaagagat 135000

ccctagcacc atctcccaca gcatccgctg catcaaaaaa gttcaccatg aaaaccactg 135060

ccgggaaaaa attttgccgt cggaaaatct ttacttcaaa aaacagcctc tcatgttttt 135120

taagttttca gagcctgcat ctctgggctg taaggtctcg ctggccatcg agcagcccat 135180

tcgtaaattt atcttggact cctccattct cgttcggctc aaaaatcgta cgacctttcg 135240

ggtatctgaa ctttggaaaa tagagcttac cattgtaaag cagctgatgg gaagcgaggt 135300

ctctgcaaaa cttgccgctt tcaaaacgct tctgtttgac accccagagc aacaaacgac 135360

aaaaaatatg atgacgttaa taaacccaga tgacgaatat ctttacgaaa tagaaataga 135420

gtatacagga aagcccgaat ccctaacggc ggcagatgtt ataaaaatta aaaacacggt 135480

gttgacactt atttctccaa accatttaat gctaacagcc taccaccagg ccattgaatt 135540

cattgcctcc catatactgt cctcagaaat ccttcttgct cgtattaaga gcgggaagtg 135600

ggggcttaaa cgcctcctcc cccaggtgaa atccatgacc aaagcggatt acatgaaatt 135660

ttatccgccc gttggctact atgtaacgga caaagcagat ggaattagag gcatcgccgt 135720

cattcaggac acgcaaattt atgtggttgc agaccagtta tacagcctag gtaccaccgg 135780

cattgaaccc cttaaaccaa ccattttgga cggtgaattt atgcctgaaa aaaaagaatt 135840

ttatgggttt gacgtcatca tgtatgaggg caatctattg acgcaacagg ggtttgaaac 135900

aagaattgag tctttaagca agggcattaa agtcttacaa gcgtttaaca taaaagcaga 135960

aatgaagccc tttatttcgc taacaagtgc agatcccaac gtgctcctca aaaactttga 136020

aagcattttt aagaaaaaaa ctcgcccata ttctattgat ggcatcattt tagtagaacc 136080

tggcaattct tatctaaata caaacacctt taagtggaag cccacctggg ataacacatt 136140

agactttttg gtgcgaaaat gtccggagag tttaaacgta ccagagtacg cgcccaaaaa 136200

agggttttcc ctgcatctac tatttgtagg catctccgga gagcttttta aaaaattagc 136260

gctaaattgg tgtccaggat atacgaaact attccccgtt acacagcgca accaaaacta 136320

ctttccagta cagttccagc catcggattt tccattggca tttctttatt accacccaga 136380

tacctcgtca ttttctaata tagatggaaa ggtccttgaa atgcgttgtc ttaagagaga 136440

aatcaatcac gtcagctggg aaattgtaaa aatccgggag gataggcagc aggatcttaa 136500

aaccggcggg tattttggca atgatttcaa aacagccgaa ctcacatggc ttaactatat 136560

ggatcccttt tcctttgagg agctggcaaa gggcccttct ggaatgtact tcgccggtgc 136620

caaaaccggc atataccgcg ctcaaacagc acttatttcc tttattaaac aagaaatcat 136680

ccaaaaaata agtcaccaat cctgggttat cgatcttgga ataggaaaag ggcaggacct 136740

aggacgttac ctggacgcag ggataaggca tcttgttggg atcgataagg atcaaaccgc 136800

gcttgcggag cttgtttatc gaaaattttc gcatgctacg acccgacagc acaagcacgc 136860

taccaacatt tacgtgttgc atcaagacct cgcagagcct gcgaaagaaa tcagcgaaaa 136920

ggtacaccaa atttacgggt ttcccaagga gggagcttct tccattgtta gcaacctgtt 136980

tattcactat cttatgaaaa acacgcagca ggtggaaaac ctggccgttc tgtgccataa 137040

gcttcttcag ccggggggaa tggtgtggtt taccaccatg ttgggagaac aggtcttaga 137100

attacttcat gaaaatagaa tagagctcaa tgaagtatgg gaggctcgtg aaaacgaagt 137160

ggtcaaattt gctattaaac gtctctttaa agaggatata ttacaggaaa ctgggcaaga 137220

aattggagtc ctgttaccct tcagcaatgg cgacttctac aatgaatatc ttgtgaacac 137280

agcgttttta attaaaatat ttaaacatca cggcttttcc ctagttcaaa agcagtcctt 137340

taaggactgg attccagaat ttcaaaactt tagtaaaagt ttgtataaaa ttcttacaga 137400

agccgataaa acttggacaa gcctttttgg gtttatttgt ctgcgcaaaa attaaatatt 137460

ttttcataag aagtactacc caggttttaa agaaatagct aaaaatatca tatggatact 137520

gccatgcagc ttaaaacgtc tattggttta attacatgtc gtatgaacac ccaaaataac 137580

caaatagaaa ctattctggt tcaaaaacgt tacagccttg ctttttcaga atttattcat 137640

tgtcattact ctataaatgc taatcaaggt catctgatta aaatgtttaa taacatgaca 137700

attaatgaac gactgcttgt caaaacactg gattttgacc gcatgtggta tcatatttgg 137760

attgaaactc cagtctacga actataccac aaaaaatacc aaaaatttag gaaaaattgg 137820

cttctcccgg ataatgggaa aaagcttatt tcattaatca accaagcaaa gggctcagga 137880

acacttctat gggaaatccc taagggtaag ccgaaggaag acgagtcgga ccttacctgt 137940

gccatacggg agtttgaaga agaaaccggg attacccgcg aatattacca gattctccca 138000

gagtttaaaa aatctatgtc atactttgac ggtaaaacag aatataagca tatctacttc 138060

cttgcaatgt tatgtaagtc gttggaggaa cccaatatga atctttcttt acaatacgaa 138120

aaccgaattg ccgaaatttc taaaatttct tggcaaaata tggaggctgt acgttttatt 138180

agcaaacgcc agtcattaaa cctggagcct atcatcgggc ctgcatttaa ttttattaaa 138240

aactatttac gatacaagca ctaggatgcc gcattaaaat gccacataag gtaatacact 138300

aggaatgtcg cacacgcaca agaatacaac gtcgccggag atttattatc tagtacacgt 138360

tttatgtatg tacaatccgc cttcatttaa tatattgagc ggatgtacta tgtatttatt 138420

ttaacaaaaa acattatttt tttttaatct tcatcatctg tttttataaa ctcagtaata 138480

tcaaaagtag cttgtggggt ttcagagggt tcaccttggt tatcctccgt gaggataaca 138540

tgttcttcag gttcgtcgtc actggagaac ccatcattta attcctcttc actcaacatc 138600

tgtaaaaaat cttccaagct ttcgctatcg ttaaaatcct catcatccat aagaataatg 138660

gtaccttcct catcgtttcc tccttgtttc gtgtctaaat aggcctgcat ggcatttgca 138720

aaagtatcaa aataggctga gtcagattgc tgttccaaaa tatggccttg cgtattaaat 138780

gtggttgcat cgttgttaaa tgcttgcaaa tacagtaagg gatttatatc cattattatt 138840

aagcaaaaaa aatttaaatt atttttcgac cgatgttagg taaaattaaa caattgctat 138900

aggtgttaag caatgtttat tgattttaag tactcaacaa ccatgatgta aatactatac 138960

agcacttttg gatttttaat caaatccaga ttaatactaa cttcttttgt gatacagttc 139020

gtaataatag tatcctgctc atcgttttgt aagatttctt ttaatatatt tttttttacc 139080

gggatactaa gcaattgatt attttctttt aaaaactcct tttgatattc aatcgtctta 139140

ttcattgaat atttgtatat aactataatt acaaatgttc aatgaattgt tattcatgtc 139200

gggagatggc tatttaaaaa tcatgtccta tttttctttg ctcaataagc atccaaatat 139260

tttcatggcg ttttattaat tgttcattat tgaacgtatc acaaagatca tttataaatt 139320

gcagatagtt tattatttct ttcaagagag taacaaacat tacttcagca gaacatataa 139380

taggtaattc agtggcgtta aaagaatttt gatcttgttg atacgccaat ggcgaggact 139440

taaggagatt tgggggtctt gcccaaaacc ctaggctgct gttcttgttt tttagggcgt 139500

cataaagaaa tgaaagcaca ttgcaaggct taagccgcga catctccttc cccttgggcc 139560

ctttccatat ttttagatct aagatctcat ccgagcttat agagtaggta tagtaaagtt 139620

tttcaaaaaa gcatatctgc ttgaagtctt ttttagaacg actttcaaga agcatttcta 139680

taatgttaac aagttttgtt aggtttaagg cctgttcctg tgtaagctcc tcttgcacgt 139740

gatagactga aaaagtgtgc ttaggaatga aaatactccc cgtggcactg gcctgttgtc 139800

tgccaggtat atagtacacg ctgctgttag caagctgtac cggcacaatt tgccccactt 139860

ctgcaacatt attttgcgat tcggacgagg gtatgacaat agttacgggt tcagtcaata 139920

ggctttcgcc gagaataata ttactgtcat ttttaataat tttaacggcc gctattaaat 139980

caaaggcatt taagtaagaa acaacagcag aaaatcttac atgcatatat cctcttccgc 140040

tattattcgt acgcataata aaacaagggg agcgttgtat aacgccagta atattaagaa 140100

taaaactgtt tttgaaacac ttacccacat aaatgttttc aagctccttc aaaagatgag 140160

cctccacatt tgtacaaaaa ttggtaggat catcaatatt caacgttgtc tcaaaaattt 140220

tttggtcgat catatctata atatattctg tctatttcaa tttaaataat atacgaataa 140280

ataacgagat tattttatta aataagcaat ggtgtataca ctttgtattt actttgagat 140340

atactttgtg tatcacaacg tgccctaaga tgtgtgcaca agtgacggca ttttgtcgtt 140400

aaaaaggtaa aaccagcgga ttccatcctg cattccattt ggttgattac gagcctccat 140460

ttctttttgc aaaaggttat tgcgaatgag taagcagagc ttgatggcac taatctttgt 140520

aaggtttaaa cttatgccca attggtcagc aattttttgt tgctcctccc gtccgcgtgt 140580

ttcgcatacg gctccccggt ttagcatgcg aatatcagta atctcattct tttttaaaac 140640

ctggataggt gggcggattt taaatttaag ggcctttccc ttgctttcca tatagcctat 140700

gacgatgtcg ttttcttttc gtttaacatt aatattaagc atataaagcg gaatttcatg 140760

ccaggtttta tcttctcgcg aggtaataag tcgcacggag tcctccgtgg catagcccac 140820

tagagtgttg tcatccccag gcacgtggct tataatttta aaaatgtccg gaaatggctg 140880

aatatctttt tttgaaaaag cgatgaaaaa ctttttataa acctcgacaa gggcccccat 140940

acctgcaaga ttatctataa taagtgcttc tagcatcgta tagtgaaatg aagcggggta 141000

gtggatgagt acctgctcca ttggctcatc ctgaaaatcc ttctgaaact tttcatacaa 141060

tacttgaaag ggttctttgg tctgcgagtg ttcgaggtat ttggtaatac ggatgctgtg 141120

catcgcggga ggctgaaaat cccgaatata tgtttcaata tctaataccg gttccttttt 141180

atggttaagc accgcagcga cgtacaaatg ctcaggcttt gccggcacat gcataatggt 141240

gcaaagacga ttctgtatcc ataattcctt gcactggttt tttgagtagc atagagaaat 141300

gagcgccagc gcgaagttgt cctctgagaa gagtttatta tcgatggtaa ttccctgtat 141360

gagcttggga gtggaaacag ccttccatag ctcggagtac gtccacacgg ggcgtgccat 141420

aaacaaagat ataataatat tagaaattgt ttttacctct tgctccccgt atccataggc 141480

ctcaaaggta ttgaggacgg tggctccgac gtttgccggc gtgatggatg gactaagggg 141540

cagactttcc aacataggct tatcaatctt aatctggttg gtgaacccat caatggcgtg 141600

ctttcgcagc gccttatccc cctcctgtat taaaatgtat tcttttaatt tttgtgcgta 141660

cttagcgagc tctggccctc catcgggtgt tgtcgatacg tacaaataaa ttgtcacgtt 141720

gcgctcactg ggggggagct ccatgtgtga attttttcgc accaccctcc caaatacctg 141780

aataagccgg ggaatatcaa ggggcaatga cataatcatc tcgtaccgca cggcctgaaa 141840

gttcaaaccc tccacaatca ccttggaccc gatgagaata cgcagctggt ggccttccag 141900

gttggacgag gcgttaaaaa gagccaggct tcgttcgcgt acagcgggct ctatttcgct 141960

gtgcagaatg gtgaaccgta ctggaataaa ctgatggtcg ctatgtgtgt gctcatcgcg 142020

aatcgcggcg cagatggagc agcgggtcgt tcccacaggg gacgaaactt catttaaaat 142080

gccattactt tgtaaaattt cttgcaagat aagaaccccc gacatgcgga cccgattgtg 142140

gtaaattaaa attttccccc ggccttgccg aataatggaa agaatgtctt tcatcatttg 142200

agtgtatttt ccgctataaa aggccaatcc cgagatgtgc gttggtggct gcagcgacaa 142260

aaagctgcca ctcacattaa agggggctct acgcgaaggc tcaataatct gtaccccgtt 142320

ttccagaagc cagtctgtgc ttgccataga aagggcggtg ggggtttccg tcgagttaaa 142380

caggccgtaa gccttgggtt ccgtttgttt tgaaaatttt gggttgggaa acaccatgtc 142440

ataaatgctg tacgcattac tcgagatttt agggtcaggg cccagctgtt taagcgtttc 142500

aagctgatac tcagacatgg ggcattcgat gaaatgtaag tacggcaatg tttcgtcttt 142560

ataggacaac atctttccgg caaatattct ttcggggtaa aaattggtgt tggtatccaa 142620

caaaaaagat acccttccgg tgctcagtct ttccacaaga gctagggcgt cctttttcca 142680

tttaacggaa tgcccactgc tgtcaaacag ttgctggcgc tggaggggct ggccgttggg 142740

cagctcatgc cgcggaacca aaaggtttaa caggtcgacg tattccatga cactcccggt 142800

tacgggcgtt gccgacatga agacggccct gggggcctgg tgaggtggaa aggcatccag 142860

gacatactgt aaagcgatgc cataattatt tcgttcctgg atattgtaca cgttgtgtat 142920

ttcatccgca atgagcagtc ctcccctaag ttgctccatg attttttgat tcacccggat 142980

gaggccgttt gtctcggcct cgctaatttt ttgcacgaac tgagatatat cgttctcatt 143040

caatgtatct tctgcttcgt cagaacgatg aaacagagaa agcacatcaa agtttttctc 143100

ttcaccctta ctcgtaatat tgaaaagctt ggatgcaaat tccttatagc cgtaaaactg 143160

aaaaaagcct ccgcggtttc tatcggttaa acggcgcttt aacgtactaa cgaacccatt 143220

tagatgccgt gattcgaccg acgtggtgct gccagactgc tttgcaatgt gaagaagccg 143280

gtgtagctca gcgacctcct tgtaagaaac aaatcccagc tcaggacgtc ttagcatttc 143340

tgtttgaatg atggcgcgtg taaagcctac cacaaaaatc cagggcgcat tttcaataaa 143400

attcatgtag tggttcataa attgacgcgc gatggcaatc gcggcaatgc tttttcccgt 143460

cccggtctgc cagtttaata aaagacgcga gtagggcgtg ttgggatttt gaaagttttg 143520

gacgaaaagc tgggcattat gcaattggag acccttgatg gaaggaaagg gcgacgcgta 143580

ggggtcacac ggaaaaaacg ctcgcccccc cttctcgcag ccaggcccac cgatctggac 143640

aaaatgagcc cgcagatcac gaatgagctc tttttggtcg acaggagggg aaatcaacga 143700

tttaaactcc tttcttcgcg ccaactgctg caaaaagtct gcggcatcca attcgggata 143760

cgccatatta tcataaaaaa aataaacctt tttatgaaaa cttttatgtg attctgtatt 143820

gcaattgttt tttatgaata ctgtaaataa gcgtatcaac ttgtttttct aacgaagagg 143880

cgttattctt tttttctgga tataaaataa taataagtat aataattaag actaaacagc 143940

aggcaatcac tatcaaactc atattatact tactttttta taaaaagtat tatatcttat 144000

gaatgcgcaa gttcagctaa ttgttcgtcg cttggaatgt gggactgcag ggaggtggag 144060

tttttccttt ttctaaagaa taccgggaaa tggtggtgag gctcaggttg ttgtacatag 144120

tagctaggag gaggtttagg tatgctcgac ttgcagtcaa tagtccggtt atagtaaacg 144180

atggcaacga tgataagaat aataatgagc aaaatcaaaa tgcccaggag aatcgcagtt 144240

gttccgggat atttggcgat tgtatgggct aaaaggcctt gggtgctttg tttaattccc 144300

tcgcgggttg acaggttatg agaaagcagt ggagacgttt cagtgtccat ttattacaat 144360

tgaacagtta tattaatctc aaataaaata taacacaaaa ttaattatgg ccatgcaaaa 144420

gttatttacg tatatttacg agtttattga atatcgtaag atggtgctgt tggaagaaaa 144480

ggtaccatat gataagtttg ttcaaatggt acttaataca ggattttttc gtattaacgc 144540

ggagacgctg aatcacggaa tcgtatccgt gtttatcttt ggagcaaatg gcaagtacgt 144600

tcaccacgga ggcgacatga gaacgctttt aacgaatacg cttaatgaaa aaaaacatta 144660

tgaagaatta attttaatcg ttgataagcc cgttttaagc aaaaaaaata ttttagatat 144720

aatcgtcgag cagcgcgctg caaatcccac gattgtaata aacatatatc cctaccacct 144780

gttctgcatt aacattccca aggtgagtgc cattcctaaa cataaactaa ttactcagga 144840

ggaggcgcag gagtttttag gtcgcgaata tctgcaaccg caggacctca tgcaaattag 144900

cgcgtcagac cccccggtgg tctggctggg aggaagaccg ggagactttg tgcaaattga 144960

gcggccctca gagacagcta tgcacgctgt tgttatccgc tttatcacca agtccaaaat 145020

ttgagtcccg tgtttaaaga tgacagacag ctaagtaagc atatctgtaa aattgtcgat 145080

gtcctctgtg gatagagcgc tttcctctga gcagcaaatt ttttcataca tctccatggg 145140

ggatggcgag gctttaatag tatgtaggtc acgtaagaac tgttgtatga tgggatattt 145200

gtcttttaaa aactggggat gtttcataac tggaattatt tgaaagataa agaccttcca 145260

tccaaagtag ccaaccacat ttggcatttc gggacacgcg gtttcataag gcatagaata 145320

gtgaatagtg tactgatctt tttgatacag cgtttcaagt agttggcgaa atgtttccgc 145380

gtcgagcgtg ccaaaatctt gaggagcctc ggtgtgctcc tgtgtagagc agatcgtgat 145440

gattccccag gcaagcggga gcatggactc tggagggtgg atatccgtat tggtctcatt 145500

attcgatccc agctgatgaa tgccgcacac gcgaaacatg gcctcgacgt agatgcccat 145560

agagataggc ggcgaaaggg caagaccgga ttgtatttgc ggcatatagt aggagggcac 145620

cgagtttttt atttttcggt tgaatgggga ctttatttct accagcacgg ggatgcgttt 145680

cgtggcctca tagcgtacgt tgttaaaaat tgttttgatt tcccaggact gttgagtgta 145740

tcccagcgtt aggtgacaaa acccatcggg gctattacta tgtccggggt atcccaaata 145800

ggtcccatca atatgaatat tgtcacctat gacggtggtt tggcagaaca actcaagcag 145860

atctttacta acacgctcaa aaagggttcc ccagctacaa gcagcgcggt tcaaattctt 145920

cttaaaaaga tttgcttttt ccgccaaggt tatataatag cttttgtaag ggtttaaacc 145980

taaaacgctg gcaaggtcag agccacccac ctgagtgcga cgaatagcat gccaggcatc 146040

ggagcgctgc tgaggagagt ctttaaacag gcgtacaaag gtttccatta tacttgtttt 146100

aacaggaatt caatataaaa agtcaacaca gtttgcaatt tttccaatct caagatatag 146160

ccatacattt ttttttccaa ttggcgaata tgtttaagct catgtgtttc aatattagca 146220

tccggaaatt taaatgcata aagatgttca aaggcctgat ttatacacgt atcaaaggat 146280

ctgtggtatg ttattagctt cagcatgtgt gccagatctt caagatggtc taaatttata 146340

cggttttcca cgtggtggat catgtctgcc acatcttgag cccccatcca ggggatcaca 146400

aggtactccc ccttaaagat gattcgtcgt ttttttaaaa aatcatgaaa acgttttaaa 146460

gcttcaagaa aggggcagtt gggctttgac cccaaaatgc tgacgacgat atcctcgggc 146520

atgatgtatt cgcagtgagg atagtagttt acggactcta attcagcggc ccgccgtttt 146580

atttcgtatc ttgcccagtt attcagagag tactccacgc ctccgaccac aacagacatc 146640

ctatctatta aaaaataaca ataaaaacct tatgaaatct atgtatagtg gccgctaaaa 146700

tgtctatatt agaaaaaatt acgtcaagtc cctctgaatg cgcagagcat cttacaaaca 146760

aagatagctg tttaagtaaa aaaatacaaa aagagctcac ctcttttttg gaaaaaaaag 146820

agacactcgg ttgcgattcg gagtcctgcg taattaccca ccccgccgtg aaggcctatg 146880

cgcaacaaaa gggactggac ctctccaaag aactggagac tcggtttaaa gcgccaggac 146940

ccagaaacaa cacgggtctt cttacaaact tcaatattga tgaaacgctg cagaggtggg 147000

ccataaaata caccaagttt ttcaactgtc ctttttccat gatggacttt gagagggtcc 147060

attataaatt taatcaagtg gatatggtaa aggtatataa gggagaagag ctacaatatg 147120

tagaaggcaa agtggtcaag cgtccttgta acaccttcgg atgcgtttta aacacggact 147180

tttcaacggg cactggaaaa cactgggtag ccatctttgt ggatatgcgg ggcgactgct 147240

ggagcatcga atattttaat tcgacgggaa attctcctcc aggtcccgtt attcgttgga 147300

tggaacgggt caaacagcag ctattaaaaa tacaccacac cgtgaaaacg cttgcagtta 147360

ccaacattcg tcaccaacgg tcgcagaccg agtgcggccc ctacagcctg ttttacatca 147420

gggcacgcct cgacaacgtg tcatacgccc attttatatc cgctaggatt accgacgaag 147480

acatgtataa gtttagaacc catctgtttc gcatcgcata aactaataaa gtttgaattc 147540

tttataggaa taaaaatgga agcgtttgaa atcagcgatt tcaaagagca tgcgaagaaa 147600

aaaagcatgt gggctggcgc cctcaacaaa gtcactattt cgggtcttat gggggtcttt 147660

accgaagatg aggaccttat ggcgttaccc attcacagag accactgccc cgctttgtta 147720

aaaatttttg acgagatcat cgtaaatgcc acggatcatg aaagagcttg ccataacaaa 147780

acaaaaaagg taacttacat taaaatttcg tttgataaag gtgtgttttc ttgcgaaaac 147840

gatggcccgg gaatccccat tgcaaagcat gagcaagcca gtcttatcgc caagcgcgat 147900

gtgtatgttc ccgaggtggc ttcatgtcac tttttagccg gaacgaacat caataaggcc 147960

aaggactgta tcaagggggg aaccaacggc gtcgggctga agctcgccat ggtgcattcg 148020

cagtgggcca ttcttaccac cgccgacggc gcgcaaaagt atgttcaaca tatcaaccaa 148080

cgcctagata tcattgagcc tcctaccatt acaccctcca gggaaatgtt tacacgtatc 148140

gagctcatgc ccgtatacca ggaactaggg tacgcggagc ctctgtctga aacagagcag 148200

gcggatcttt ccgcctggat ttaccttcgc gcctgccaat gcgcggccta cgtgggaaaa 148260

ggcaccacca tttattacaa tgataagcct tgccgcacgg gctctgtgat ggcgctagcc 148320

aaaatgtaca ccctgttgag cgcgcctaat agcacgatac atacggcgac cattaaggcc 148380

gacgcaaagc cctatagcct gcaccccctg caggttgcgg cggtcgtgtc ccccaagttt 148440

aaaaaatttg aacacgtgtc cgttatcaac ggggtaaatt gcgtaaaagg agaacatgtc 148500

acctttttga aaaagactat taatgaaatg gtcgttaaaa aatttcaaca aacgattaaa 148560

gataaaaacc gcaaaacaac attacgagac agctgttcaa acatctttat cgttatagtg 148620

ggttccattc caggaataga atggaccggc cagcggaagg atgaacttag catcgcggaa 148680

aatgttttta aaacgcatta ctccattcct tctagttttt taacaagtat gacaaagtct 148740

atcgtggata ttcttctgca atccatttct aaaaaagata accataaaca ggtcgacgta 148800

gacaaatata cgcgtgcccg caatgcggga ggaaaaaggg cgcaggactg catgctactc 148860

gcggcggaag gggatagcgc actttccctg ctgcgcacgg gactaaccct gggaaagtcc 148920

aacccaagcg ggccctcctt tgacttctgc ggcatgatct ccctgggagg agtcatcatg 148980

aatgcctgca aaaaggtgac aaacattaca acggactctg gagaaaccat tatggtgcgc 149040

aacgaacagc ttaccaataa taaagtgttg cagggaatcg tgcaggtatt gggtctagac 149100

ttcaactgcc attacaaaac acaggaagag cgagcaaagc tgagatacgg ctgcattgtt 149160

gcgtgcgttg atcaagatct ggatgggtgt ggaaaaatcc ttggactgct gctggcctac 149220

tttcacctgt tttggcctca gcttattatc catggtttcg taaaacgact gcttaccccg 149280

ctgatacgtg tgtatgaaaa gggtaagacc atgcccgtgg aattttacta tgaacaagag 149340

tttgatgcct gggcaaaaaa gcagaccagc ttagccaacc ataccgtaaa atattacaag 149400

ggattggcgg cgcatgacac ccatgaagta aaaagcatgt tcaaacattt tgacaacatg 149460

gtgtacacgt ttaccctgga tgactcagca aaggagttgt ttcatattta ttttggcggg 149520

gagtcggagt tgcgaaaaag agagctttgc accggcgtgg tgccgctcac cgaaacccag 149580

acgcagtcca ttcatagtgt ccgacgaatt ccttgcagcc tgcatctgca agtagatacc 149640

aaggcttaca agctggatgc catcgagcgg cagattccca acttcttaga cgggatgacg 149700

cgggcgcggc gcaaaatttt agccgggggg gtgaaatgct tcgcctccaa caaccgtgaa 149760

cgaaaggttt ttcagttcgg gggctacgtt gcagatcaca tgttttatca ccatggcgac 149820

atgtcgttaa acacaagtat tataaaagcc gcccagtatt acccaggctc ctcccacctc 149880

tatccggtat tcataggcat aggaagtttt ggctccaggc acctgggagg aaaggatgca 149940

ggatccccaa gatacatcag tgtgcagctt gcgtctgaat ttattaaaac aatgttcccc 150000

gcggaggact catggcttct cccctacgtc tttgaggacg gccagcgggc ggaaccagag 150060

tactacgtgc ctgtgttgcc gcttgctatt atggagtacg gcgccaaccc atcggagggc 150120

tggaagtaca ccacttgggc ccggcaactg gaagacattt tggccttggt gagggcctac 150180

gtcgacaaag acaacccaaa acacgagcta ctgcactatg caataaaaca taagattact 150240

atactcccgc tgcggccctc caattacaat ttcaagggcc atttgaagcg gtttggccaa 150300

tactactaca gctacggcac gtacgtcatc tcagagcagc gaaatataat tactattacg 150360

gagcttcctc tgcgtgttcc tacggttgca tacatcgaaa gtataaaaaa atcgagtaac 150420

cgcatgacat ttattgaaga aatcatcgac tacagtagtt cagaaactat tgaaattctg 150480

gtgaaattaa agccaaatag tcttaaccgt atcgtggaag aatttaagga gactgaagag 150540

caagattcca tagaaaattt tctgcgcctg cgcaattgtt tacattcaca tctaaacttt 150600

gtaaaaccta aaggtggcat tatcgagttt aacacgtatt atgaaatttt gtatgcgtgg 150660

ctaccttaca ggcgtgagct ttaccaaaag cgtcttatgc gtgagcacgc ggtgcttaag 150720

ctgcgcatta tcatggaaac tgctattgta cgctacatca atgagtctgc agagctaaat 150780

ctttcccatt atgaggatga aaaggaggca agccgcattc taagcgagca tggatttccc 150840

ccgctgaacc acacgctgat catttcccct gagtttgcct ctatagagga actcaatcaa 150900

aaagcactgc agggctgtta tacctatata ctatctttgc aggctcgaga attgcttatc 150960

gcagccaaaa ctcgtcgggt ggaaaaaata aaaaaaatgc aagctcgtct tgataaggtt 151020

gagcagcttt tgcaagagtc tccctttccc ggcgccagcg tatggctgga ggaaattgat 151080

gcggtggaaa aggctattat aaaaggaaga aatactcagt ggaaatttca ttaaacgcta 151140

ccggttttat gatgtccaat aggtgttaag caatcagttc atcaacattt ttttcaagaa 151200

tttgaaaagt ttggataatg ttctgaatac ttttttctaa aagagttatc aaatcttctt 151260

gtgaggcctt atgaataatt gttaatacca tttcttgctt atggggaaca cactgatacc 151320

ccacaaagct aatatcagga atcatttcat aaatatatgt ttttagcaga tttccgatgg 151380

tatgggtttc atcttttatc gtgataatgg cctttgtttt ttcctcatcc atggaaaaca 151440

gcacaagttc cggctgcggc tcttcaaagt tttcataaat tttttgaatg ctttggattc 151500

ggccaataat gatccggcag gcgtttttta aatacgtgcg aacggcctgg ttgatatgtg 151560

gcagcggcac cgctggaaag caaagcccca ggcggtggtg acgcgggtct gaggtcatag 151620

agctttgctt gtaaccgcta agcgccatat attctttttt atccgttggg tactgttcaa 151680

tgtcaaggtg ggaaaaatgt gttttaacgg caagattaaa ggcggcatgc tttcgtccta 151740

tgcccttttt aatatagata tcctctataa tcaacgattt tccgggttgt aggaagccaa 151800

tctcaaaggt aggattaaaa atcgggtatt taagcttagg gcctgccacc tggatgagat 151860

cgcggctata gatggtttta acctcacagc tattgtttaa actccgcaga gcaaatacca 151920

gtgtctcgtt tttcgcataa atcggaatga aattaatgcg gtttctaata aattgttccg 151980

tcataaacag gtccgtggaa tcctcgatct tatacccacc gggcttaata tctagcatat 152040

aattgggaat ttcatcttgc aagacccgcg acaggccgtg gaccgcggct ctgctaatgc 152100

ccttaaagtc cataacaaca ttgaccggga cgaggggcaa ctgctcctcg agctgaaata 152160

gttttttggc cgcattttta ataaagaggt tggaaaagtc tatcaaaaac ggtttgattt 152220

ccacgttttg gaaaattttt tccatttgta ttataaatat atctatatat attcaaatta 152280

tggtagttta tgacttgctc gtttctttaa gtaaggaatc catagatgtg ctacggtttg 152340

tagaggcaaa ccttgcggcg tttaaccagc agtatatttt tttcaatatc caaagaaaaa 152400

actcgatcac gacacccctt ctcattacgc cgcagcagga aaaaatttcg caaattgttg 152460

agtttttaat ggatgaatat aataagaaca atagaaggcc ctccgggccg ccgcgtgagc 152520

agcccatgca cccattattg ccgtatcaac aatcctcgga cgaacagccc atgatgccgt 152580

atcaacagcc cccggggaat gatgatcagc catatgagca aatataccat aaaaaacacg 152640

cgtcgcagca agtaaatact gaactgaacg attattatca acatattctt gcattaggcg 152700

atgaagacaa aggtatggac agcatgttaa aacttccaga aaaggcaaaa agggatagcg 152760

atgatgagga cgacatgttt tctataaaaa actaacgacg taacaattaa acaaaaaata 152820

aaaatcatta taaaatgaat cttgaatacg tccaagttgt tcaaaaattt aatcaagtac 152880

tcctagaact taccaaaaaa gtatgtaccg ttgtgggcgg gagcaaaccc acctattggt 152940

atcaccacat tagaagggtt tgctcagaat gtccatccat gccgatgagt atgataggtc 153000

cgtatctgaa tgtctataaa gcccaaattc taacaaggga caagaatttt tttatgaatt 153060

tcgatcccgc gcataatgag tacaccttta tcattcaaaa actaaaagaa gcagcccgaa 153120

atatgccgga agacgaatta gaacagtact gggtaaaact tttattttta cttaaaagct 153180

acataaaatg taagcccttt attaattaaa gaattgatgc ataactaata aatggccggt 153240

cgtgttaaaa taaaacagaa agagctcata gactctactg taaaaaacaa aaatgtgatg 153300

aatctgttcc atgaaattat aggctcaaaa ggcaatatta attttagcgt tgtctggccc 153360

aagtttaaaa aaatcaaaca gagcgtttat gactacattt ccactctttc tgtgctggaa 153420

aaagcaaacg ttatgcaaaa ctttgaagct gataagaaac tgttggaact ttttgtacaa 153480

aagctgtggg ctgcctatga aggctatttc aaatatcccg agattgaaaa atatgaggtg 153540

gaaggccagg taaatttcaa tctcgtacct cagtgcgtcc tcgaaaagtt tagccagttg 153600

tataggataa gaatcaattc agagcttgtc acactcatcc taaacagctg tgcctttatg 153660

agtaaatata acgattatat tctcaaaaaa gatccctaca tactaaccat aacccccggc 153720

ctatgctttt cccccattcc caacttcgag gacctaaatt ttaaacatct ttacaacagt 153780

gataaaaatt ctcagcatga caaagagttt atcatgttta tattatataa gctttatacg 153840

gctgccctag gagtgtacaa tgccatctcg attccagaca tcgacgtaga agaccttgaa 153900

aatatcatcc tatcctcggt gagccagatt aaaaaacaaa ttccgcgctg caaagacgcc 153960

ttcaacaaaa ttgaatcttc ggtacacctg ttgcgcaaaa attttaacac atattacagt 154020

gactatgtgg gctcaggcta caacccaacc atcattatgg aacagtacat taaagacata 154080

tcacaggatt ccaagaacat atcaccacgc atttcctacc agtttagaac catcatcaag 154140

tattaccgcg acatgattgc caccaggcat caaacgatgg acccccaggt attaaacctc 154200

gtaaagcacg tcgaaaagaa attagatatg cttgatagag aaaaaaatta gtatatatag 154260

ttatggtgaa tctttttcct gtttttacct taattgtgat tattacaatt ttaattacga 154320

ctcgagaact atccaccacg atgcttattg tttctcttgt aacagattat attattatta 154380

atacacagta tacggaacag cagcatgaaa acaatacatt tttcatgccg caaaaaaatt 154440

cttttaacga atcttataat aaagacaaaa aatctaatat acatattccc taccagtggc 154500

tggcgcctga actgaaggaa gctgagagca agtactggtg gggcaattat gatcctcata 154560

gcgagcccgt tctcgctggc gcatcttgaa tatcttcata cgtggcacgt caccatcaaa 154620

aacattgccc aacagcacgg gcttgatata aaggtggcca ttgtggtctc aacatcgcat 154680

ttaaataatt ttttgccaat ttccggggcg cttaacatcg aatgtataac cttccccagt 154740

tgcggcatca aggagataga cctcctatgg gcgcgcatta aactatttca acattactgc 154800

gccatcggtg cccgtctttt atggctggta agtgctgaca tcaggccccc tgtttcagcg 154860

tggccagcca tcgccgacag tctaaaaaag ggagcagatg cggtcgttat tccctacccc 154920

tcccgatgga acaatcttat acctaccgtc atcaaagaaa tagttgtcca ccaaaaaaaa 154980

tgccttgtgg cggtggatgc acgccacctt gatacagata cccagattgt aggggccggg 155040

atgggctgca tcgtcctaac cctaaaggcc cttatggtgc gcctaagtat tggcaaacag 155100

cccgttaaga tactgtggcc cgaccttcac ggcactgccg agggcattcc tctggagggg 155160

gtggaggttg gctggttttt aaacgcttat gcgcataaat taaatatacg ctgcctaggg 155220

gctgatcata ttgcgcagca cttaacttaa ttctttattt aaaaagtcca cgcatccagt 155280

ggcggcctac attaagggcc tacgcacata aatatacact ggctagaagt acgccttcat 155340

ttaaaccatt gaattattta tataatggct gcaaacatta ttgcaacaag agccgtgcca 155400

aagatggcca gcaaaaaaga gcatcaatac tgtctgctag actcccagga aaagcgtcat 155460

gggcattatc ccttttcatt tgaattaaag ccttatgggc aaacaggcgc aaatatcata 155520

ggagtacagg gctcacttac ccatgttatc aaaatgacag tatttccatt tatgattcct 155580

tttcctttac aaaaaactca tatagatgat tttattggtg gacgcattta tttatttttt 155640

aaggaactgg acatgcaagc agtttctgat gtaaatggaa tgcaatacca cttcgagttc 155700

aaggttgttc ctgtaagccc caaccaagta gagcttcttc ctgtgaataa taaatataaa 155760

tttacatatg ctataccggt agtgcaatac cttaccccaa tcttttatga tctttcggga 155820

ccgctagatt tcccattaga tactctttcg gtccatgtgg atatcctctc caatcatata 155880

cagcttccta tccaaaacca taacctaaca acgggtgatc gtgtttttat ttctggatat 155940

aaacacctgc aaacgattga attatgtaaa aataacaaga tttttatcaa aaatataccg 156000

ccgctttcat ccgaaaaaat aaaactatat atactaaaaa atcgaatcag aattccgcta 156060

tactttaaat ctttaaaaac gtctaagtaa taacattttt atagtctact cctagttccg 156120

aaataggctg aatttctttt ttaagtcctt taaaccaagg atgtgataca agactcttaa 156180

aggaaagccg cttattttca ttaattgtta aacattccgt gataaactgt tttcccgtct 156240

ctgaaatgtt ctcgggaata taattttccc gtttcaggat atcatttaaa taaaaatttt 156300

ctgcacgaaa tctaaaaaga ttaaccgcga ccatacctat cgtccacacg gttaaaggaa 156360

gctggtagta ataaccataa taataaaatt ctggacacac gtattcccat gttccaaaca 156420

tattatattg gggacgggtt tcgtctaatc taacagcgct tccaaagtca atgaccttaa 156480

tgatcttttg atttatgtct ataataaggt tctcatcctt aatatcccca tggataaagc 156540

ccttctcata aatgttttgt ataataagaa taagctggaa tattattttt ttggcttcgg 156600

tttcctcaag ttttttaaag taatgataat gaagtagatc aacactattt ggaatatatt 156660

ctatgattag tatatgatac atagcatttt cggtatattc gataagctta ataacaccgg 156720

gagtatcttg cagggctttc aacacgatga cttcatttcc tggaatttct tttttagaaa 156780

cgtacttaaa tataatgggt tgccctactt gatgacccaa aaagacgtta tttctgccac 156840

cctcaaacat gggtctcgtc gcaatgaaat acatgtgctg cgttgtggag atcctttcca 156900

cctttgctgt aggataaaac gcatattgtg cctggggatt ttttaacatt tttttaagct 156960

gttgttccgg cctggacatg ttttattagc tttatatata aagggttaga aggtttaatt 157020

tcaatatatg ccttaatgat gggattatat tcgtaaaagg tatagcctaa tcctacgtct 157080

ttgttttttt ggtaaaaaaa ctgtttgccc tcgtaggata tgctataggc ttttacttcg 157140

gcttttacaa gcggttggca gggattgggc aaacgtaaat cgcgttcaaa gttttcatga 157200

aaaagcaaag catttgtggg ctgacacatc agacagccgc tttcgccatt gaaggcacat 157260

tcaatggccg ccctttttag taaatcgcgg aaagcagaat taagatggct cttttcaagc 157320

cccctttcgt gaaaacgctc atcaatcgtt ttttgttcct gactgccttc gggaatacta 157380

taaaacattt tttgattagc caccgcgatg tacaaaaaag gctgtacggt tttctcctcg 157440

ggcggtagcg catcgtggct accaatgcgt ataatgcgcg ccttcacttg atcctctcgg 157500

gccttatccc agtacggctc taggatatga acctgccgcc cgtatttgag atccaatccc 157560

tcagctcctg ttttagagac gagtaaaatt ttaataacct ctccgtgtat attcagcggc 157620

gaattccaaa gctgctggat catgtcgcgc tctttagata aaattttccc tgtaataagc 157680

gtaaatcgtg ttattttgga ggacaggact aacgtatggg tcggcccatc ttccgcaaag 157740

tttttcacca taagatcttt cccatcctta tgaaggagga tggtgttgtg cccttcttcc 157800

aatactttta ggggctgaag gcactggtag ccctctattt ctaaaaagcg ggccacgacg 157860

tgaaggccca attccacaaa ctgtgagtaa atgagcacag ggcccggaga cgttttaata 157920

ttttttagca tgcgtactat tttgggacta gaattttctg tgaaggcctc tttgggcagc 157980

tgctgaacag cctctgataa tttttcatcc tcctttactg ttagcatttc ggacgcgaag 158040

atgctgatca tacgggaacg cacatagtag gaggagcctg actcttgctc cgatcctggc 158100

aggcagaggg cggcggcatt tattttttca tacattcctg agctggcgtg cttttccgcg 158160

ttttcaacgt ctcgggccag cagatattgc ctatactgct cgggtgacat ttcaaccttt 158220

tctataataa gaggaagctc tgtggggaat agcttgttga gctcattctg gtttccagcg 158280

tagcttatca tacccactag gcggtttagt agtttgtccg cgtttaaagg gctattcgtt 158340

gttttattga cataagcggt gtagaatctt tcatagtgaa gaggtaataa gattcgcccg 158400

cttagcatat taaaacaggg caccatttca aaggggtcct tcgaacacgg ggtgcctgtt 158460

aaaaacagaa tacgaatatt tttagcttgc ataatattat tgtacagctg gcgggcattt 158520

gttttatcat tggcgctatt gataattcct ctaaagaggt tgtgtgcctc gtcaacgatg 158580

agcaggcatc catttaggga ccctcccgcc tttatgatct gctgccccat gttgtaagcg 158640

tctagggaca caaacctgaa gcgccgcgag attttttgta gctctttgga gtgatccgtc 158700

gtttccggat ataaaagttt aataagcttt aacaaagact gttggaagtt tgagtgcaac 158760

gacttgggtg cgatcagaat cgggttgtaa atatgtgaaa gtgagatggc aagcgacagg 158820

ctcaaaatgg ttttccccat gcccatctgg tgatagatga ggaggccccg tgtgttttcc 158880

ccctggccta tcccaaattt aggatccgaa aaggcggtgt aaattaaaaa ctggtagtat 158940

ttcagggctc gtgcaaagcg ggcagtgagt gaggtgtctt tgctttcctg aagctcttta 159000

tatttttcat atacctcttt taggtatgct tctatttgga cggggaagga ggtgttgttg 159060

tgcacgcaag acatgactcg ttataaggat cccatattaa aacttcatta gaagaatagg 159120

gctgctgata gctagcgctg cacttaaaaa tggggtagcc ctttttcttg taaatccggt 159180

gcctgtcgta gacctggcta gaaagcgggc ttagtgtatc tttaatgtcc acaacgatgc 159240

gtaccttttt ttcatccgat ccctgccggg taatacgtcc caagatttgc tccatgttgt 159300

ttctgcgggg cgttgccatg atgatcgatg tcatatgctt gaaggaaatg cctctacgcc 159360

cgtagccata ggtcagcaag ataatggaag cgctgtgtgc ctgagaaaga gcggtatttg 159420

aaaccccgcc gcataggagc gccacctccg gaacgataat ttgaacatct ttgaattctt 159480

tggaaagcgc ctgataaaaa atttctaaaa gtttgcgaaa ttccacgaaa atgatgatgc 159540

catacggctc atcggtcccc catttgtgag gctcagcggt atgcagggag taaagccgct 159600

ttgcctcatt tacgacaagt tgtatacgcg aaggatcttg aagtagttta tcaatggtgg 159660

caatggccga taccttttca ttaatataca cagggctaac gaagtcagga tgtccctgat 159720

attcgatttc cctcacgtac ccggaaaagg ttgtggtggg acttacagtc ctctggggct 159780

gtcctagatg gtgaataata atcttgtcca taccatcggg ccggtccagg ggtgtagcgg 159840

acagtcctaa tatccgacta agttgtattt tccaaaaaat tttgtaattc tccggcgagt 159900

gtaattcatg tgcctcatct aacacgacta gaccaaaggg ctcaaagaac tgctcaggct 159960

tcttgcgcag ggtattaatg attcccacga tgacgtcgta ctctttgctc gtcatgtcct 160020

ttttcttgca cgctgcatta ttgtaagcag ctacacgtag gtggggcagg agcaatgtta 160080

gctcgtcgat ccactgtatt tgaatcgcct tggtgggcac gatgaccagg gtagggtaca 160140

aaagtttttg aataatgctg atcgcaatac gcgttttccc caaaccggta tttagatgta 160200

ggtaaaagcg cccatagggg gacaggagct ttttatgaat cttatcgacc atttcttgct 160260

ggtagttaaa tagtggaaat tctgtttcaa cgcatgggag ggcccgcagc gacacggggc 160320

gcgtcgtgta aaccatgtta aacatttcaa actgcttttg cagcaatatg ggaaaataaa 160380

tgtattcccc ctgcagcgtg aaggcagttt cctgtcttat ggctatgtgc tttggctgcc 160440

cgggtaatgc ccgcgccgta acggtgagcg ccttaagaac gcgcccgaaa tcatgttgta 160500

atttactttg tagcttctta taatttattc ctattccagc aaaggatata atggcctcca 160560

ttctcacgct ggacgggtta tatgcagagg ttccaaaatt cttaccagag gcgttacgag 160620

agggctgtgc tggcaagaat cctctaagct tttatattca acaaatttta aatttaatgg 160680

gatgtgacgg taacgagtac catgttcttt ttaccagcag ctccgaggaa gcaaatactc 160740

atatgatcat ggccgccgtg cgtcgccatt tgctgcggac gcagcaaagg cctcatgtca 160800

ttatcggagc agccgagccc cctagcgtca ccgaatgtgt gaaggcattg gcgcaggaaa 160860

aacgctgcgt atacaccatc atccccctaa aaaattttga aatagatcct gttgcggtat 160920

acgatgccat acaaagcaat acctgcttag cgtgcatttc aggcactaat gctgttgtca 160980

aaacgttcaa caaactccag gacatcagca acgtgttaaa aggtattccc ctgcactcag 161040

aagtgagtga tcttgtttat caaggatgta ttcaacaaaa tccgcccgct gatagttttt 161100

caataaatag tctctacggc ttcctgggag tcggtgtttt gggaatgaag aaaaaggtca 161160

tgcaaggatt ggggccgctc atttttggag gagggctgag aggcggaagc cctaatatac 161220

ccggaattca tgccatgtat aaaacgctaa cccagcaaag gccttctatg aaaaaaataa 161280

atacaataca tacgctgttc atgaaaactt taaaaaaaca tcagcatgta tatctaccca 161340

tagggggcgt gtctgcagag gacacgtctg cagaaaacat atctacaaaa gacatgcctg 161400

ttgaaggccc gaagggactc ccgggctata ttttatttag cgttggccgt cgcgccgagg 161460

agctacaaaa aaaaattttc actaaattta atataaaggt tggccgtgtt gttgacttac 161520

aagagatact gtttcgtatc aaaatacccc aaaaatactg ggagacatta ttgttcatcc 161580

aattaagaga taatttgacc aaagaggaca taaaaagagt tatggttgtt ttgatgcatt 161640

tagataccat cactcctcgt ggctctcttc ctcctccgag ccactcttct tctttttctt 161700

aatcgttttt gtttgttcta taataaggga aaagaactcc gtgggatctt gttccccgta 161760

caggttatct gcgaccataa ggatgcttag aatggtaaac aggtgagaat acataagggt 161820

ttgcgtttta agaaaaccct gacgttgaat cataattgaa aacaccttgc aaagccgact 161880

catcagttgt tctgtaatgg cgttaagcat tttctggaat ttttcttggt tttcgggtgt 161940

gattttatat tcatgtagaa agtgtttcac acctgaggag aagaatcttt cctccttcga 162000

gagcccatct ttgatgatgg gaagttcctt gatcagggca aaccattcct cctcttgggc 162060

ttgcggattc tgaagatact gatggcagat atggtttaga atggtgcaca cgtagctaat 162120

aagctctgag ctgattcttt ggttggtttt caaatgttgg cgaaagtagt ttttcaccga 162180

agtgcatgta ataaacgtct tcattttctt ataatataca acagtatgtt gagtctttaa 162240

tttaaaatta caaggagttt tctaggtctt tatgcgtata ggtgtttctt tgtcgtaaat 162300

tttcaatagc cgacattgtt tgtgaagcag tgttctgagt agtgactgtc gtgtaaggct 162360

cagccggatg agcaggagca ctcgcggccg caggtgcggc cgccggcccg ccagttgcca 162420

tgactagtct gtccgtaact gggttgtccg taactggttt gtttgttgct ggtctgtttg 162480

ttgccggtct gcccgtgact ggcttgccta cacttgctgt agtcgctcca gctggtttag 162540

aggtacctgg ttgtggagtg acttctaccc actgctgatc ttgataagga tttataaact 162600

gtatatcttc ctcctcaata gcagcagctt ttttctttct tgaagagaat agatagatta 162660

gaacgatgat aatgatgact aagaccacga tagcaatgag aatagtatac atatgtgtgg 162720

agaagaagct tggtgtagtg actggtgaca aacactcacc ataatgccgc ggataaaccg 162780

gttgaaaaaa ttcagaatcc atttaagata ctattataaa taatatataa aaatgttgtg 162840

gcgcaatgaa attacagaat ttatggacca actttccaag tattctcaag aaatcttaaa 162900

aacgtttaag caattgcgtc ctagtgaata taaacaatac aatgaatttt taacacaagt 162960

tacaccgttg ctgcaaaaaa cccctgaaaa aattccagag ttggttgacc atatattcaa 163020

ttacctagac aacgttgaaa aaatttgtga gctcctcgtg aatgctagct caattattat 163080

tagttcaaaa atacgagaac aagtaaaaca cggaatgagc ttcagctata aagccgacct 163140

cgactccttg gcggacattc tctctcaaaa acagtacgtg cttatgcatc tttcaaaaaa 163200

tattgcggcc gagtatttta atacgtgttt aaaccaaggg aaatccaagt tagatctcaa 163260

agctgcctct gtattttata gtagtcgttc ccgaacggca agctcagcag aactctatag 163320

aaaaatgcta tacgcctatg gttcaccgca ggaaattaat tattatactg aaaaagcccg 163380

aaataagacg ttggatgtgg aggagagcga cagcatggcc atcatcgaac gaacggcccg 163440

acacaacctt tcccttatgc acccgctaga agccatgggg cttacctttg gggcaaccaa 163500

cacggacgcc gacccggagg atctgaagga caaaacggtg ataaatttaa cgctcccgca 163560

ggcaacagaa agcatcacct accatcttaa atccctaatg cagctaaaaa aagtaagtac 163620

ggcttcagga ctaaatacaa acattttgaa agcatttgat aatattattt ccacccctgt 163680

gaaaaaaaat aaaatggcct ccaagttggc gcccgggatg gatgtcgtgt tcactagcga 163740

taacggaaaa acatttttta ctaaaaacat tttaagcaaa aacatgctag cggggcccaa 163800

agagcgggtg tttgcatata ataatctcat tagtaattta aataactcct gtttcataca 163860

aaatcacaac gattttttaa gacagcagga ctcttggccc ttctatgacg cgcacaattt 163920

taccaacaag tttttaatgc agcctatttt ttcggggcag acccgtcctc ggcttcaggg 163980

agccatggag gcggcgcatg tggaaacgca tctcacggca tttttacaaa gtattcagcc 164040

ctctaggcca caagatccct ctgttttggc ttcccccaag ttatctgctc taatcttgaa 164100

ctaaaaacag cctttcttgg acttaaatga tggtctacca gtttttgaaa taacttagag 164160

aactatgaag attttcatga aatttaaatt agagatttgc aaaggttact tgcggtcatt 164220

ttctgttgaa ttaaataatt attcgaatag tataatgtct gaagatattc gtcgtggtcc 164280

tggcagaccg ccaaagaaaa gggttgttcc caactttgag cgcaagggca ttctggaaaa 164340

accagttcgg ccacaaagcc gtctcgagtt ttcctatgat aacccgctga tatttaaaaa 164400

tctttttatt tactttaaaa accttaaaag taaaaatatt ttggtgcgat gtacccccac 164460

cgagattacc tttttttcac gtgaccagtc gcaggcaagc tttgttattg ccaccatcga 164520

cggaaaaaac gtgaaccatt attacgccag tgatgtcttt tggctaggca tcaacagaga 164580

gctcgttgaa aaaatgttta acagcattga tcgctctttt ttaaaaatta ccatcgttca 164640

ccgctatgac aagcctgaaa ccctgttttt tatctttacg gattttgaca ttgacaagga 164700

gtgcacgtat cagattacgg tctcggagcc cgagctcgat atggacctta tcgaaatgga 164760

aaaaagcatc agtgaagaaa gactcaagaa ctatcctctg cgctgggagt ttacctccaa 164820

gcagctcaag aaaacattta gcgacttatc aaactacacc gagctcgtga ccattgaaaa 164880

actcggcggc gatacgccgc tgcacctgta tttccaaaag tttaactcca tctcatacca 164940

cgagatgtat aaatcttcca acaagatcaa cctgacctcg accattccta agtcgcaggt 165000

gttccagata aatgttaaaa ttgctcacat caagtcgctg gcctcggcta tggtcaccga 165060

caagatccgc attctgtgcg aagaaaatgg gaacctaatc tttcaatcgg aaatggatgc 165120

ccttatgtta aatacgatta ccttgaacac cacgatatag ttcggtaaca ttagatgttc 165180

taatatttag catctaaata atacgctgta gtccggtcag ggttgcgtca cagttttccc 165240

atttttttgc ctcgtcggcg gtggccaccg ttgccctatc atttacgccc ggtaagacaa 165300

agctaaaggc gttcagcggg gcttggcaat gcccgcccag cgtgaaggag ctcggaggat 165360

tttgcgcatc ccgaaatccc ttagccatgt tgtttaacac ttcggttacg tcaatcgagt 165420

gaagggatcc cttgggatcc gtgaatgtaa agacgcagtt tctaaagcgc atgtatgcga 165480

tggacgattc atcgggggtt ttgaaggtaa cagtgttccc cttgctgtac ttaaaggggg 165540

accatccggt aaaattatac caaatgaaag caataataat taaaataacc aacacaatag 165600

ttatagacaa cacaaagtct gtagtgccgc ccattattaa ataaaaatat tttagaccgc 165660

cggcttaaaa tttacttatt gctcatagct taagtctatt ttattcatag cttaagttta 165720

ttgctcatgg cttaagtcta ttgcttatag cttaagtcta ttttattcat agcttaagtc 165780

tattgttcat ggcttaagtt tgttgctcat agcttaactc cattactgat agcttactga 165840

tcatgactta aataaaaata ttttgcccgc ttaaaaattg tttaggtttg aaaaaataag 165900

agatggaggg ggcaacttat cgtcattgtg tttaccccca ctggaagaca tcaaacggta 165960

aataattata agaatcaaaa tgattaatat aagggttaaa aaaggatgat tcatcacatt 166020

aattaaaaac gtatttataa cgctgttgca gttgaaattt tggtataggt cggaaatatt 166080

gcccgagcct ccgtattctg caatgttctg acatatggtg agtccggagg ggcactgctt 166140

gttggtcaaa atatttcttt gctccgttgt tttataggca tttttatttc cattacacgg 166200

agcaaacgca cattcagccc atagggtgcc ggagttcaca caggcacaat actggctata 166260

cgcatactca tcctttgagc acaatccctg tttatcgcat atgctcccaa taatattgtc 166320

atcctccgcc gtttgttgat ttgtatgcga gcgtaaaata gcggcccagg ccttgggctc 166380

ctttttttgc agctcggaaa tcgaagggcc tgtacagcta aagtcgaccc aaatatcatt 166440

gcatttcgtg gaaactggca tgcaagacat aattgaaata attaataagt atatatcatg 166500

gcaacaaatt tttttattca acctatcacc gaagaagctg aagcatacta cccaccttcc 166560

gtgataacga ataaacggaa ggacctgggg gtagacgtat actgttgctc cgacctagtg 166620

cttcaacctg gactaaatat tgttcgcctg catattaaag tagcatgcga acacatgggc 166680

aaaaaatgcg gttttaaaat catggcgaga agcagtatgt gcacccatga acggctgctc 166740

atccttgcaa acggaattgg tttaatagac ccgggttatg tgggcgagct catgctcaag 166800

atcattaatc ttggcgacac cccggtccaa atatgggcca aagaatgttt ggtgcagttg 166860

gtggcccaag gtgaccatgt gcctgaccat atcaacatcc taaaaagaaa ccaaatattt 166920

ccgctgtttg cgcctacccc aagaggcgag ggtagatttg ggagcacggg cgaggccggg 166980

attatgagaa cttaatttta ttttttttct taacataatg ggaggctcta caagcaaaaa 167040

ttcctttaaa aatacgacca acattatcag caattccatt ttcaatcaga tgcaaagttg 167100

tatttccatg ttggatggca aaaattacat aggcgtattc ggtgatggaa atattttaaa 167160

ccacgttttc caggatttaa acttatcatt aaacacaagt tgcgtgcaaa agcacgtaaa 167220

cgaggaaaat ttcattacaa atctttcgaa ccaaattact caaaatttaa aagaccaaga 167280

agttgcgtta acccaatgga tggacgcagg aactcacgat cagaaaacgg atatagaaga 167340

aaatataaag gtaaacttaa caaccacact tattcaaaac tgcgtttcat ccctgtcggg 167400

tatgaacgtg ctggtggtga aggggaatgg caacattgtt gaaaacgcaa ctcagaagca 167460

gtcgcagcaa atcatctcta actgcttgca ggggagcaag caggccatag acaccacaac 167520

cggcatcact aacacggtaa atcagtactc acactacacc tcaaaaaact tttttgactt 167580

cattgcagac gcaatttcgg ctgtttttaa aaacatcatg gtcgcggctg tagttatcgt 167640

tctaatcatc gtagggttta tagccgtctt ttactttttg cattcacggc accgccatga 167700

ggaggaagaa gaagctgaac cactcataag caacaaggta ttaaaaaatg ctgccgtttc 167760

gtaataattt aattaaaagt aaaaaaaaaa ggtattgtta tagtgatggc agattttaat 167820

tctccaatcc agtatttgaa agaagattcg agggaccgga cctctatagg ttctctagaa 167880

tacgatgaaa atgccgacac gatgataccg agcttcgcag caggcttgga agagtttgaa 167940

cccattcccg actatgaccc taccacatca acttccctgt attcacaatt gacccacaac 168000

atggaaaaaa tcgcagagga agaggatagt aattttctac acgatactag ggagtttact 168060

tcactggtcc ccgatgaggc agacaataaa ccggaagatg acgaagaaag cggtgcaaaa 168120

cctaaaaaga aaaaacattt gtttccaaaa ttaagctcgc ataaatcgaa gtaaaaattg 168180

aagcgaaaaa aagtagaaaa aaaatgtttg gagcttttgt aagccaccgt ttgtggtcag 168240

atagtggttg tacgaccacc tgcatcacaa acagcattgc taattatgta gccttcggcg 168300

aacaaattgg atttcccttt aaatcagctc aggtatttat tgccggccct agaaaggctg 168360

tgataaatat tcaggaagat gataaagttg agcttttaaa gatgattgtt aagcacaatc 168420

tttgggttgt tgctcatgga acctacttag atgtgccctg gtcccgtaag agtgcgtttg 168480

ttacacattt tatacaacaa gaactactta tatgcaagga agtcggtatt aaagggttag 168540

ttttacacct aggcgctgtg gagcctgaac ttattatgga aggactaaaa aaaattaagc 168600

cggttgaggg ggttgtcatt tacctggaaa ccccgcataa caaacatcat acatataaat 168660

acagtacaat tgagcagatc aaagaattgt ttttacggat acgaaatacc aggttgaaac 168720

agattggttt atgcattgat acggctcaca tctggtcttc cggtgtcaac atctccagct 168780

ataatgacgc ggggcaatgg ctgcgctcgc tggaaaacat tcattccgtg atcccaccaa 168840

gccacattat gttccaccta aatgatgccg ccacagaatg cggaagcggt atagaccgac 168900

atgcaagtct ttttgaagga atgatttgga aatcatatag ccataaaata aagcaaagcg 168960

gtttatattg ttttgttgaa tacgttacgc gacaccagtg tccggctata ttggagagaa 169020

acctcgggtc ttccatgcaa ttacaaaccg ctttaaccgc agaatttact acattaaaat 169080

cgttattaaa ataaggatga gttttagcga atgtccctta gttattagtg catgcaaaaa 169140

atttctacaa aagcgtatta caatagagaa tgaagcactt ataaatgcct taataaccgc 169200

tttagcgcag accagcacgt tgaatgatct ttgtttatta cctattcaaa cctatttgct 169260

tagttataaa aatgcttttg agtggataca cttcgtatgt attgcaatca ccactatttt 169320

ggataataag tataactgga aggactgtac ggtagatatt aattatattt ttctccatgt 169380

aacctatatt tacaatatta aaaccaagga atacctagac tactgttctt aaactttatt 169440

ttttctatat ttacgccaaa gagaatattt aaagtttttt ttgaaaaaaa ataatatatg 169500

tagataaaat tcagttacat gatatatgtg taaacatgtg tggtaaacaa catatggtta 169560

tgctttataa gataaatgcg cataatatat gtaaacaaaa tatggttatg tgttaaatgc 169620

atataaatgt attttaacgt atatcttgtg ataatggata tatgcattta ttaaaagagg 169680

ctgtatttat tataaatctt gctaaggatg ccattgtcaa catatatccc atgttggaca 169740

aattgcgttg cgatccagtt cttttttttt tgattttgtt taatgctatc ctttttgaag 169800

ggatggttgt ccaccatatt tattcgatgt tcaatgaata ggtctgcttt ttcgtaaggc 169860

agtgaaggtc gttccaagac tccttgaacg aaggacgtgt tttcttggat ccacttaaaa 169920

agcacgtggc attcaaaaac aggacagtga ttggatcctt ggatatgctt tggacagcca 169980

atgcttgaag agatgtagtc ccttttcttt aggacaagct tctccacgct ggggcaacag 170040

agatcgttca agttctggac ggtcgcattt ggaatgttga aacttcgtat ccattcaccc 170100

tcgggtcctc ccttatgaag aaggagtatt tgctcatggt ccttagtaat cttaaccaaa 170160

tgttggaaga tcattttttt acctgcttta aaggcctgaa gggtgtcagt tggcaaagct 170220

attgaattcg ggagtgggct ttcatcaagc gtgaaatggt gaatgtgacg cgactggaaa 170280

gaaaacgacc gttgatttat tttttcaaag attgggtcga ttccgccatg aaagaacagc 170340

tgcaagattt tagaaggcgt atttttttcc caataaaaaa tgaccacttc tcgtgggatt 170400

aaaatcgtct gtgtcccatt ttcattatat aattggccca taaagccatc aacgtcaatc 170460

aacaccaaaa gcatggtata gagagctttt agaaccggag ttcgttaaaa aaatacaaag 170520

ttcgtttaaa acgtgtaatg ttactaaaaa aatgtaatgt ttaaatgata atgataccac 170580

atgcattaat gaaaaaaact tttaaatttt tgttttaata tttgcatgaa aatggaaaca 170640

tttttagtct gtttatttca caatgcagat ggtttacatc aacagattca ggaaattttg 170700

tatttattgc ggatgcatat ttacgaaaca aatctttact taaagcagga actatcacgg 170760

cttatatatc caaataggca actttctttt gtgttactta tgcccctttc ccttctaaga 170820

aactgggatg acattgaata tttaacggac gttgtagatg ataagcagac tctacattac 170880

gcggcaaatt tgctgacaaa ctacgttcta catctatcca tgtttcaaaa gctgacaaaa 170940

ccatacttcc ttttagcggt caagcgggtc agcgaaaaac tcaacaaaaa gcagcgacat 171000

tcattttacg aggtattggt aacctccgaa accttgaata attatgaaaa cctatctaaa 171060

aacattttaa atacgttgat gtttgccgtg cgctacgtat ttaaacctac gccgaactat 171120

tcagaaattc tcgcagagtt ggaaaaaaaa aataaaattc accatattat ttttaatatg 171180

gtaattacgg attttgcgca aatccgtgaa caacaaatgg ataaacatct gtgtgaaaca 171240

aataatgagc ttcgtcagga atgtaaagaa actatttttg atttaaaggt ggtaggaaat 171300

gtttagccaa taaactcatg cccgcatttt ttacaggtac aaaatatcgt ggatggctca 171360

tcgagggcgc gtgtttgtac ttctctgtag gtacacatac gctgcttgca gttgggacac 171420

ttataaagtt gtgacgtctt ttcggcgacc ttttgctgcg aacgtagagt aatttctgtc 171480

ttctccttta aggcggcaga ggggcaaagc tcggcgaacg tcatgctacc aattgcctcc 171540

ggttttagct cgccagaaat tagcttatta agggcatcgt tatcctgttg ttggtgactt 171600

tttttttcgc agttaataat atgattgatc gtcccacaac gggttgaata ttcttctaaa 171660

aaggtttttt cttgttgctg gtacgtataa tgataacacg aggcctcgat tttttgcgcg 171720

tattcggtgc ataaatcagt atgttcctta aaaaacatat gtttttgaag cgttctaaaa 171780

aacatcattt ggatgatatc acgcatttcc aaaataatat agggttctag tcttttggaa 171840

tctttcataa ctagatcggt ggtaatattc ttagtcatac aatttattaa aaatggttta 171900

atatattgta aatatttttt aggcgtgtca gcctgtaaaa aacattcttg ttcaatctta 171960

tttgtaagga tagtattttg caaatactta tttagcaaaa atacgataga atcgcgggct 172020

atatgcattt tcatataatt ttttttttaa aatttaatac aaaaaaaaga agtatagact 172080

cttcttctag tccggttagt tcgttggttg cctcaacatg gagactcaga agttgatttc 172140

catggttaag gaagccttag aaaaatatca ataccctctt actgctaaaa atattaaagt 172200

agtgatacaa aaagagcaca atgtcgtctt acctacagga tctataaata gcatactgta 172260

cagtaactca gaactttttg agaagattga taagacaaat accatttatc ccccgctttg 172320

gatacggaaa aactaattgt aaccagtagt acatttaagg atagtttaag cagtaaatgt 172380

agaataacac agttaagcaa taaataacaa gtatatagga atatatagga atatatagga 172440

atatatagaa atatatagaa atagctaagc ttaatactaa ttcagctttt tttttaacta 172500

aaacctgaat agatgcgaag tagcggacat atacatacta aaataagcca tacatttact 172560

ttcttcttga acatgaaacc tttttttctt ctgttgttgg tatataaaca ataggactgt 172620

ttgctgaggt tgtatgatct tctacaactg ctgtctcagg atgacgatgt ttttttaaac 172680

taaaagtgta ggatggaatg agtggaatat agttatggct cgacttatcc tgtttcgtac 172740

aggaatattt tttacaaata gaacgcaaca agcatatgaa taaaaacaga aatgatatac 172800

aggagcataa aatagatatg aacactaagg ggtagcagct tttataacgt tccgtatttt 172860

tcttagctat caattgattt accgtaatat ttatctcggg aaactttgtt ctacaatatt 172920

ttgtttggta ttccagaaac tcatgtcctg gcttattccc gcagcttaaa aaatgataca 172980

aaaatgtgtt attgttacta aaattaattc ttcttaagaa aaactgcgga agacgcttta 173040

ggtacgtctg ttcctgtttt agtaggaagt agtataaggg acaatttctt tttccacaca 173100

ttagattatt gtaatatagg taggttgggg tgttggagcg aataagtttt ctgagtatgt 173160

tataatctat gacttgtaaa tcgttatacc ttaggtccaa aaacttgagt tctttaccaa 173220

agccacctgc aatttcagaa atatttttca tcccgcagcg gataatacgg atgtcctgaa 173280

acgtctttaa aatacttgta ttgtagtgaa tacttatgtt atttttttgt aaataatcta 173340

tgtcatgaca agtgcatgaa atgccagcag cattgcttgg tatagtatta tatgcaggaa 173400

gaactatact actattgaga atagtcacat tgtacttata ccatgtatta ttttctgata 173460

taaagtattt gcaggtgacc tgtggtttaa tcctacctgt taagccactt cctaaaaaaa 173520

caaaaaatat gaaaaccctt agcatcctgt atatactatt aaaaatttat aaaattttct 173580

gtttaaattt catttagaca aaaaaaataa tatatataca tcagcaagaa attatataca 173640

gattatataa ttttctgatt tttttttgcc acaataagca tcattatatg cattaaaatc 173700

tcaatactaa acactaaaat ctaaattcta agcattaaat tctaagcatt aaattctatg 173760

cactaaactg taagcactaa aatctaagta actaaaatca acactaaatg tatgcaacct 173820

aaaatgtaaa gcattactca tcatcctcct cttcttcatc ctcatcatca taggttaaga 173880

tatatgtgtc atcctccatt tcttcacatt catcttcata agcatcactg ggtattggtg 173940

gaacattgga tgcagcattt ttaaaatatt ctatgtcttc tggtgaacac tcatctaatg 174000

attttttgac agtcctttta acttccatgg gatatgattc caaatcctct ttatataaga 174060

gtttacggta gcttttagct gcatccacat ttgctggaga atctggattt ggctcattga 174120

gcagtgaaat tacactaaga agaatggtat caatcttttg agccggagac caagtcattc 174180

cctgttcttc agcattgtct ccgtgtaaga tagagataca tagttttcca tcagagtaaa 174240

tattaggatg ccacatttca gaggtgaatg ttaatctggg tggtgcatat gggtattctg 174300

gaggaaaggc gatttttgcc ttgaataagc ctccctcata aaaagtgtca ggtgggcccc 174360

ttaagatcac atcccattca gtcatatcct tctcattcac cgaaattttg aaattctcag 174420

agggattctc tatcaggtgt ctgtactctg ctattaaaaa cctggaaacc atggttattt 174480

aatattaatt aaattccctg gtttattcct ccttaaaagt agatgaacct cttttgtttt 174540

ttattgggtt catttttact aaatttatga actggaaaaa actttaacgg cataattatc 174600

aaatgcgaag ggggatccgt ataaaatcct agcttgccgg taatggctat taagttaaat 174660

ttggtaccag taacactaat atttaaaaag ccctgatcat taactttcca cattaaaaga 174720

ttattatatt cgaatgtttg tccaatatgg acaactttgt caccagatgt tacatttgat 174780

ttggttgtta gtggctgaag cttggcacaa tcaaaaataa gcccattaac actaagatat 174840

agaggagtgg gttgatctat tttctcatag tttaatattc catctttcca cgtaatagct 174900

tgataattat ccgcagcaat gagttgaaat tttataaata gtacaggggt tttagttgtc 174960

gttatacatt taaagggtgt tttataaaaa taaaaaataa taattgttaa aagtatgata 175020

ataatcgcca aaataatttc atacattttt tataagaatt atacatagta tggtatttaa 175080

aatattagct aaatttaaaa aaacttcatg atttttaaaa cagggaaaaa ggggattagg 175140

ttgaataaaa aaggtaagca cttgtctata tatttttttt acaatgttgc cttgagtcgc 175200

atttttaact ggctggggag tatcagagtg gaatatcact gtagtaggtc tataaggtct 175260

tgttaaaata tgatcggtca ttgttttcgt actagtgtca tttagggtcg acctgatagc 175320

tcgatataaa gttatagggg ataacctatc aaatacagtc ttatctgtgc tgaaatgtat 175380

atcgtcttct ttatcactaa taatattagg aatggctgtc attaaataat tactacttgt 175440

tgttgtgggt gaaatagttg tactggtatt attggaaatg gctgtcatta aataattact 175500

acttgttgtt gtgggtgaaa tagttgtact agtattatta gaaatggctg tcgttaaata 175560

attactacct attacaagta aactaatgct aactacattt ttaacctcaa taaacctaaa 175620

aagccatact aaatacctaa acaacatcct gttataatat gagcagaaaa aaaaaataag 175680

tataattagg gaattattct tattcgctta ctattaagaa taattcagaa tcttatttag 175740

ttagaaacta tcataaagtg aataggactc atcgtcggat gaagattccg tttcagagat 175800

agtttctttt tcttcctcag aataatctgt tcctacaata gaatcggtgt catcctcaga 175860

aagagaagta tttaaatatg gactatctat agcaatatcc tcttctatct cgcaatcctc 175920

ctcctccatt tccatagtgt gtaggagaat atttttatca tcatgctcac ttcttttttt 175980

gttgaaagat gaaccgtcct caatacggtt catgttaagt tccttcatct tatgtataat 176040

ttccgtaatc cgtgatgttt ttgacatgta agatggtttt aaggttatat ccacaataac 176100

aggagaatct ctatcatttt catttgataa actttgatct ttgatttctt cgtctaaaat 176160

tcttgtcttt ttttgggtac tagatgaaat agaggaattc atattctgaa acgatatatc 176220

aaggggagct ggacgctttt ttccaattaa accgtttttc gagatactat gattagatga 176280

atgatcttta gccaagctgt ccttggatat actatagtta gatattttac ctttaaataa 176340

tattcttcta tacaagttat tcttaggtaa agaattagta tggattccta tatttttatc 176400

tgaaggagtg tccatatcgg agaacgtcct cttacgaata ttttgaccac gagccatttc 176460

atccactata ggcagtattt tggctggcta tggttctttg ttgtgacaat tctatgagat 176520

ttgattgcaa atcaattttt agttttaaat atattggtac ctaggacaaa gaaagtatat 176580

atagccaata attattccac taaattgatt tccagactga tgggtatgga gccatgttgt 176640

ctctgcagac gatcgcaaaa atggccgtag caacaaacac ctactccaag tatcactatc 176700

caatactgaa ggtctttggg ctgtggtgga aaaacaatac gctaaatggc cctattaaaa 176760

tatgtaacca ttgcaacaac ataatggtag gagaatatcc tatgtgttac aatcatggaa 176820

tgagtctgga tatagctttg attcgggcag taaaggagcg taatatatcc ttagtccagc 176880

ttttcaccga atggggggga aatattgact atggggcact ttgtgctaac actccatcta 176940

tgcaaagatt atgtaaaagt ttgggagcca aaccaccaaa gggccgaatg tatatggatg 177000

ctcttataca tctttcagat accttgaatg ataatgatct gattaggggg tatgagattt 177060

ttgatgataa tagcgtgttg gattgtgtca atctcatacg actcaaaata atgcttacct 177120

tgaaggcccg tatacctctc atggaacaac tagaccaaat tgccttaaaa caacttctgc 177180

agcgatactg gtatgccatg gctgtacaac acaacttaac aatcgctatc cactattttg 177240

ataatcatat tcctaatata aagccattta gtctgcgctg tgctttgtat tttaatgatc 177300

cctttaaaat ccatgatgct tgcagaactg taaatatgga tcctaatgag atgatgaaca 177360

ttgcttgtca acaggattta aactttcaaa gcatttacta ttgttatctt ttaggggctg 177420

atattaatca ggctatgcta atgtctttaa agtatggtca tctttctaat atgtggtttt 177480

gcatagattt gggggcggat gcctttaaag aggcaggggc gcttgctgag aaaaaaaata 177540

aaagagtgtt acaacacata ttaggtctta atatctttaa gcgagagttg attcccccct 177600

gtaaagatcc tgatccttat caaatccaaa ttctgttaaa aaactacatt ctaaaaaatg 177660

tctcaactgt ttttacatat tattgccagt agccattgtt tatatcagaa aataacccat 177720

ttgtttatct ttttttgtgg ggcaaccatt aagacccgac gcaaaaaaag attaatcttt 177780

tatcagatac ctaaaacgtt ctataaggga gtctatgaga tggatcatat tttgatggtc 177840

atagtaagaa gcaagctttt tggcgaaaac aacggagtta aagaatttaa cccgctcatg 177900

tttggatagg acttttaaca gcgagccaaa acagtattta aaaatttggc aatagttttt 177960

ttgggatgca ataaacaaac acttgatcag tgcccgcttc actttctgat cagacatgtt 178020

tgccgcataa caggcctttt taaacttagt aatataatta tgttccgcaa gcaccattaa 178080

caagggaacg atgggaagct gcttttcttg gtgaaattta cgtaaatatt cgatggccac 178140

cgcttggacg actgtgtaat ttactaagtt agaaatgata gctttcatgg ttgtaaaaat 178200

atacatagga ttttcttttt ctgtatacag tttgaaaagc ttatgattac gtgaaatgat 178260

ggccattttt aatacaagat ggtatagtgt atctttaggt aaaaatgcct tgcaagccgc 178320

gatgatgtcg atgttgtctc catgaacagc gatagaaact aatgtttcca atctaaatgt 178380

ttttatctgc attaatagaa gaatgcagtc aatgttatta tacttaataa tactgtaata 178440

caccgaatca atgaccgtca tctgagaatc aagctgactt attagtaaat ttaacgtttt 178500

tttggaggca tgacctttga tcgcggcact aagtgcacac agtatagcaa aattgttaaa 178560

tacattttga tttaggagaa ggagtaatat tttccttcgg ttatagtacg cagcatctgt 178620

gatgattatt ggccgataaa tgttaaaatg tgttaacagc tttttaaaaa aacggaagta 178680

atttttttgg atcgctgttt gcatcatcga aataatgaga taatcagggt atataatggg 178740

taggtcacat gctacctcta acaaagaata gtcgcccaat ctaaaggctg tgttgaaaag 178800

cgtactatca tcatacgtat cgagtacccc tgctgttaca aaccaagcga taagatgaat 178860

gtgccgttcc ttgcaagcta tcgcaaatag ggagtttcct atggaatgtc gaataatgta 178920

ctccctattt ttttccaaaa tgtttggaaa attgtatagc gttgcggcat acagtagaca 178980

ctccattctg gcgttataat ttttactttt acatatgaat aggtggaaga actcgaataa 179040

ttcttgagaa cttgttaaat gcataatatg gtgatatttt ggtgtcgtta aatggtatga 179100

gaaaatgcat tctaatacat cttttcggtt atgctttagc gcctgagcta aggcatattc 179160

aggctcgacc cataggacta gtgtttctat aattgagata ttcgcctgct ttgccagggc 179220

atactttaag acgctccggt tagaaaaaat gttgttatga agatggataa ccgtatccat 179280

ttttacgatg ggaccattcc agtatagtcc taaatgctgt agcagatctt ttgttagttg 179340

tgaagcgttc tcgggtgtca tataaatatg ttgcagggct tttttctgta aggagaacat 179400

ttcgtcgtaa tcgtacaaaa aaaaattaaa atttgggcat ggatgattca aacataacaa 179460

aatcaagatt ttataacagt ttgcattaac ctatacatat atgcaagtaa atgagatatt 179520

atctatcata acgaatcaag ggatatttgt atatatcagg agtttctgaa ataaagatat 179580

gaagattatc atagtagtat ccatcaatca caatgcaact tcctttaagg cataatttag 179640

taaactcagc actcccatct tctggatgct ttacaactaa cattaaaaac tcctcagtca 179700

tattatctgt aataaaataa gatcctcctg gagccatttg tagcatgtct cttattccta 179760

caaaatcttt tttgggatgg taaaaactca gcagtttcaa actctttttt agtttttttt 179820

cctggtattt aagccatttg ttataaaaca gttttcttat gaaaatgcat ttgaaaatat 179880

tgggaatgtt taaccatgct tcttccgagc acatctccag atacttactt tctttgtttc 179940

ccatgtctaa tttattgctc actaagttag taatgaatct attttaataa tctactttac 180000

taatctatct taataaccta tcttataatc tatcttaata acctaattat aacctattta 180060

taattggcta atgctgccgg catttcatgc ctatctaaac aactcctact aagcaatcta 180120

ctattacata tatagattca ctttttatat ttgtaaatca tgagaattat aaaatcatta 180180

ctcattttta ttgtaaatta gtgggtattt gtaaaaatct tcaaacgttt taagatagtt 180240

ttctagagag aagtaatctt tgccatcaat atataatgct tttcctttaa actccagttt 180300

tgctatgttt agtgagccgt ttctagatct ttttgggcaa taaatagatt ttcattggtt 180360

gcatcgtccg taagcagaaa ggtaccacta ggcacgttaa aaaacatacg ttctatttca 180420

tggtcggatt tttgagaata gaaaaaatct aattttttaa tccgcgttaa ctctttttta 180480

tcaatctttc cagactgttt tatatatact ttattgcaaa tcttacaatc ctctatggct 180540

tcattatact tattttgctt atcctctatt gacatgtccg tatttgatag gtaacttccg 180600

ttaaggcggt tccccatggt tttagataga tttttaattc agttgtatac ttttattatg 180660

aggctaaaat atagaagttt gatcctaaaa aaataaaaag attttgtaca tttatttatg 180720

gtttatagcg gtatagaggc cgataaaagg tatccgggta gtctcctatg atatcgtcaa 180780

ttttggtata ataacagttg ttatggtagt attgtccaaa ccgagtatgt atgcgccggt 180840

gaagcgtccg cccgctaatg gtacagttcc aggttaagac aatcatatca cacccaaaaa 180900

gagaggaaac agcataggtg cccaaaggtt cattatataa catacgccgc atatatttta 180960

gttttttttc tccatggtaa taatcacagg ttttcatgtc ctgcttaata ggatgattcc 181020

ccatgtatga taatatataa taaatttagt ttttagcttt ttcaaaaaat tgggcgctcg 181080

aaactaaatt ttccttatca cagcgtttgg agaaagcgta tttaaagata tatcttcttc 181140

taacaagact gcaaaaaaaa tcttacccct tatttttata atgttcatca tagcgtttga 181200

agatatcaga aggtgccagg ttttataaaa atatccttta ggatttataa cgatacaagg 181260

gtctataaaa tatatgcggg tataatctta taaaatcatc gattttttca taatattctc 181320

cgtttataca ataaagatca taacagatat tgatgcgtag atgcattatt cgcgtgttcg 181380

ttgggcagct aaaggatatc acaacgtagt tttttttaag aaaagacgaa actacataag 181440

tccctaaggg ttcattgaat agtaaacgcc atatttgttt taaattttgt tgttcaccat 181500

agtagtattc gcactttttc aagtcttttt taataagcct attccccatg tatgcttata 181560

aataaaaatt tagaaatgtg ctatattatt tgttgatgaa tcatgaacac gtcttatatg 181620

ttgatatgtt actttaaaaa catttgtatt ttcaacagac gcgttctatt cttattaaga 181680

atgatgccgt ctttatttta aaccttggtt taaaatttaa agaagtattt ataaactata 181740

atcatgggaa ctttttcagt aactgcctct gcaaaaagtg acgatgctgt ttgtaagtat 181800

ttagaagaac caatagatga aaattacaga aacatattaa gaaatgagca tgttaaaaaa 181860

aatttaaatg aggctctgaa tcgacatatt actacctata atccagtagt tgattggtgt 181920

aataactatt caacattttc atctcaggat ttcgatgaat ataaaattta tatacatagc 181980

gatcttatgg atggacgacc tcgtccaaaa aaaacatggt gtgtcatcat gtaatgtttg 182040

ttagttttat ataaacgcaa aaatattctt ctaggagatg ttgatatact acctattgaa 182100

ttcaatatat taaagtacat ttctggctat tcccattacg gtattattat tactattttt 182160

aagagctaga tgtggattta agtaataata acattctccc gttcctccta gagacacctc 182220

atcaaattcc catcctatgc aacctttatg ttgtaaacat aatgattgac agcattcatc 182280

ttcttttgac caagtcgtcc aaatcctacc aagatctata cgtgtttttc caaatggaga 182340

ttgaagatca gcagtagtgg cattaaacct ataaaaacca ggtgcataat cacatgaacg 182400

gatcgtagga tctaatttaa tatcttttat atcttgtttt actgcttcta gacaactttt 182460

atcagtacat gttccacgta cacagtggtg tcctttatcc ttacaatccg tatctgtctt 182520

acattttttt ttcggcggtt tatgtttcag atggtaaaaa cccagtatta aaataatcac 182580

aagaataatt cctataagta cttgaacaac aggataaaac attttaatat taaatatatt 182640

ttttaattaa atgaatagat ttaatccaag tagtattaaa attttttaga aatagtgttc 182700

tacaaataat gaaatgaatg gtccaaaaaa aataaggtgt acaataatgt aatatattgt 182760

taggctaagt aaatttaata ttttaaagta tttggaaaaa tattttttaa catatgatgt 182820

ctaggaatat tttttagaca tttaaaacca tatagttact ttatttatta cactgaactt 182880

gaaaagactt attacctaaa atattaatag atgaagtaat attgtgtaat tgagtccata 182940

acatgggtgg gaaacaaaaa tctcgtaata tgaaaaataa acatcctaaa aagagtgcaa 183000

ttgttataag tttatgtaac tttattttaa agtaagaata taaaaatatg agtacaagag 183060

gaataggggc cattactaac attggctcca acatcctgtt gtctacaaaa aaaaatattt 183120

tttttagcaa aaaaaaatcc atggaaggat attaatacac ataattattt gacatcacat 183180

tagtgtactt accaaatagt aatatacaac catcctaata ttcaccttta tgaaatgatc 183240

ccaacctata cggtaaaata gtataggttt taataaagaa aaaagatatt ctgtggtttt 183300

tatttttgta tagtgtgtga atacaaaata aaatcccaaa ttttaacctt ttcttttttt 183360

ttctatacag gatgttagaa atagtattgg caacgctgct aggcgacctg cagcggctcc 183420

gggttcttac ccctcagcag cgggcagttg ccttctttcg agccaatact aaggagctag 183480

aggacttctt atgctcagat gggcagtctg aggaggtact gtctggcccc cttcttaacc 183540

gtctactaga accctcaggc cctcttgata ttttaaccgg atatcaccta tttcgtcaga 183600

atcccaaggc aggtcagttg cgcggccttg aggtcaagat gcttgaacgg ttatacgatg 183660

ctaatattta caatatactg tctcggctgc ggcctgaaaa agttcgcaac aaggctattg 183720

agctatactg ggttttccga gctatccata tttgtcatgc tcctttagtt ttagatattg 183780

tacgatatga ggaaccggac tttgctgaac tggcctttat ttgtgctgct tactttggtg 183840

aacctcaggt aatgtatttg ctctacaaat atatgcctct gacccgcgca gttcttacgg 183900

atgccatccg gataagtctt gagagcaaca accaggtagg gatttgctat gcttacttga 183960

tgggaggcag cctcaaggga ctagtctccg ccccactgcg taaacgtctg cgcgccaaac 184020

tacgctcgca gcgcaaaaag aaggacgttc tttcacccca cgacttctta ctgctgctcc 184080

agtagctttt tttgccgcag gagcaccgcg gataggagct cctccacgct cgcgatccgg 184140

cgctggaagc ggaaccgatc gaccgccacc tgctcccagg gacccttgcg ctcgatgtcg 184200

tcggcttccc acacctcgac ggctgtggca aaatggacat gcttcgcgtc gttcgtccgt 184260

tttttgcgcc gcctccccat tattcttcct gtaagattag tgtttaatac ctataataac 184320

ataattttaa gatttaatat accaaaactt aaactatttt tgtatagtaa ctattagcat 184380

gtctacacat gattgttctc taaaagagaa accggttgat atgaacgata tatctgagaa 184440

atcagttgtc gtggataatg cacccgagaa accagctgga gcgaatcata tacctgagaa 184500

gtcggcccgc gaaatgacat catcagaatg gattgctgaa tattggaaag gtataaaacg 184560

tggaaatgac gtgccatgtt gttgtccaag aaaaatgacc agtgcagaca aaaagttttc 184620

agtatttggt aagggatccc taatgcgctc catccagaag aataattaaa aaaaatattt 184680

tttttagcaa gtttttaaac tatttaaata aatgtggtaa aaaaattcac ataataatta 184740

aagtgaacgt gttagaatta atattttttt ataatcggat ataatatcca ttaaatcaat 184800

aaatgatagt gttgctacca cactaaacaa taacaaacag aaacgcacga tacctttcct 184860

catgatttat aatagcgtgt tatctaaaga tttttttgaa aaaaatatta aattttagtt 184920

gattattttt ttcagttaca acattgcttt agaaaaaata cctaattact acatagcaaa 184980

taaagcgagc gcattgttac aaacaacatt tttttgcgcc tggatactcc tatatatgag 185040

aactataata cggtatatta atcctattac caacattgtc aataatagta tgtaggcaat 185100

gacatacttt aaataccaaa tatccatggt tatttctaaa aatcttgaaa aaacgttaaa 185160

ttttagatcg gtcacctacg acagtaatac taattttaat aattgatgac tgaaatcata 185220

atataatgcc gtgcgaaaaa taattatttt tcggttaaag ataccattac ataaaaaata 185280

tgccatctac tctacaagtg cttgctaaaa aggtattggc cttaggggag cataaagaaa 185340

atgaacatat atctagagaa tattattatc atatattaaa gtgttgcggt ttatggtggc 185400

atgaagctcc gattatactt tgttatgatg ggagtgagca aatgatgata aagactccaa 185460

tctttgaaga aggcatatta cttaatactg cattaatgaa agctgtacag gagaataatt 185520

atgaattaat aaagttgttt actgaatggg gagcaaacat caattatgga ttaatttcca 185580

ttaataccga gcatgcccgg gatctatgtc gaaaattagg agctaaagaa atgcttgaag 185640

gaaatgaatt tatacaaatt atattcaaaa cattagatga taccaccagt agtaatataa 185700

ttttatgtca tgaattattc accaacaatc ctcttttaga gaatgtaaat atgggggaaa 185760

tgaggatgat aatttattgg aggatgaaaa atttaacgaa cctattatta aataatgact 185820

ctattagtga aatattaact aaattctggt atggtatagc agtaaaatat aatcttaagg 185880

atgcgatcca atatttttac cagagattca tggacttcaa cgagtggcga gtaacatgtg 185940

ctctttcttt taataatgtg aatgatcttc ataagatgta tataacagag aaggttcata 186000

tgaataatga cgaaatgatg aatctagcct gcagcattca agacagaaat ttatcaacca 186060

tttactattg ttttctattg gggggctaac atcaatcaag caatgttaac ctcagtatta 186120

aattataata tttttaactt attcttttgt atagacttag gggctgatgc ctttgaagag 186180

ggtaagaccc tggcgaaaca aaaggggtat aatgaaatag tggaaatctt atcattagat 186240

atcatttata gtccaaatac tgacttctca tcaaaaatag aacctgaaca tattagttct 186300

ttgttaaaaa acttttatcc aaaaaatctg ttcgcttttg atcgttgcaa ccccggttta 186360

tattattctt agaggaccgc tacaaaaatt attttttttt cttgatcaaa gctccaaaat 186420

aattattaga ttaaagtcgc ctatagcagc agcccactcc aaaaaaagta ttttatagta 186480

caaaaaacac gaaaaatagt ttgcggccgg cggcaaacta tttgttgttg tctaaaactt 186540

aatgtttttt taatattttt aaatgcaacc atggattgtt ggactatcag ggagaagaac 186600

tatagctaca tcatattgtc aatactggta atactattaa tatggtatct tatacttaac 186660

tattgtcgat cgaaaaaaaa tgcagttaca aacaacatgc cgccaccata cacggtgtca 186720

agtagctgtt ctcaataata gggttgattg acgctcttcg taataatatg ttgattgacg 186780

catcataaaa tgctgtggtt gattaatatg ttgattgtcg cctactttat tatataagta 186840

atgatttttg tataaaatac gggtttgtga gggctttatt ttttcttatt agaacaaagc 186900

atgcaattta aggcctacag caagagtaat ttaacaccta caacagtaat tttaaggtca 186960

gtaataatgt ttaattaagg cctgaccact aaaacttaaa cgattttgta aaaaaaaatg 187020

tctactccac tttctctaca gactcttgtt aaaaaagtgc tggccacaca gcacatatct 187080

aaagaacact actttatttt gaaatattgt ggtttatggt ggcatgaagc gccgattacg 187140

atttgcattg atgaggatag ccaaatattg ataaaatcgg caagcttcaa agaaggctta 187200

tctttagata tcgcattaat gaaagtcgtg caagaaaata accatgattt aatagagttg 187260

tttaccaagt ggggtgcaga tatcaactct agcttagtta ctgttaatac ggagtatacc 187320

cggaaccttt gtcagaaatt aggcgcaaag gaagctttga atgaaaggga tattttacaa 187380

atattttata aaacacgtca tcttaaaact agcagtaata ttattttata taatgaattg 187440

ttttctaata atctcctttt ccaaaatata gagagattga gtttaatagt ttataggggc 187500

ttgaaaaact tatcaatcaa ctttatattg gatgatattt catttagcga aatgttaact 187560

agatactggt atagtatggc gatattatat aaccttactg aagccatcca atatttttat 187620

caacgatata ggcattttaa agattggcgg cttatatgtg ggctttcttt taacaatttg 187680

tctgaccttc atgaagtata taacttagag aagacggata tagacattga tgaaatgatg 187740

aagttgacct gtagtacgta tgatggtaat tattcgacta tttattattg ttttatgttg 187800

ggggctgaca tcaatcgggc aatgttaacc tcggtaataa actttcatat tggtaacttg 187860

ttcctttgta tagatttagg agctgatgct ttcgaagaca gcatggaact agcaaaacaa 187920

aagaataata atatattagt agaaatatta tcatttaaaa attattatag ttcaaatacc 187980

tctcttttat caataaaaac gacagatccg gaaaaaatta atgccttatt agatgaagaa 188040

aagtatgagt caaaaaatat gttaatgtat gaagaattat ctcattgata caaaattatt 188100

ttttataaca gaactctctg atggtgacaa atctccgata ggaatatatg acgtaacata 188160

attatttttt tcgcccagaa aaaaattata aatgttatta ttgccagcac ttttatcaac 188220

tatacgtaca aaaaggtgtt gaccaaaaaa ataatttttt ttcttgatca aagtatgtaa 188280

acgcccgctt acagcaagga tcttaagtga gagccattaa attttattga tagctgcttg 188340

ccaccagtag aatacggcca aaccacctaa caggaaatac aaggcggccc ttcggccaat 188400

aaggtggata aaaatcacgc ataagacggt tgtaacatag cactttagtg cgaatatcag 188460

gaatgccaat agcatgtaga taaggcacca aacatcgcag ctatacatgg ctaaagatca 188520

accagaaaag gtttaaattt taacgccggc ccaaaactta aacttttttt gatattttta 188580

agtgcagcca tggattggtc cggccatagg atgacctatg cctacgtggc attctcattg 188640

atggcaatag caataatatg gtatattcta cttatctatt gccgatcgaa aaaaaatgtt 188700

gttacaagcg gtaatacgct cgctttagcg ccaatatcgc atatgtgaaa aatgttcgcc 188760

gaaaaaaaca ttaaaattta gaaccgccgc ggcatctcag gggcggcaac attttttttt 188820

atatggatat tgtcacacac cacctcatct atgacgcaat atattactgc taatatcagg 188880

ttccccaata gtatgtagag aaaccacaca agatagatat tcatggcgat ttttgacgaa 188940

aaaacattaa gttttagctt ctttgacgcc tgtgtactaa taatgtttaa cgcctgtagt 189000

ataataattg atacctacag cagtaattga tacctacggc gataatgtct ctctggccgc 189060

cccaaaaaaa agtatttacg gtagggttta ttaccggcgg cgtaacacca gttatggtca 189120

attttgtctg gcccgccgcc cagccgcaaa aaaaaatcaa ttacaaccgc aaaaaaaaat 189180

atttccggcc gcggcgtttc aaaaaataat ctttgcgaaa taattccgca tcttgtgaaa 189240

tgaacgccta cagtaataat tttaatcttt gacacctaca gcagtagtaa taattttaat 189300

ctttaacgcc tgcagcagta ctaatatttt taatctttaa cgcctacagc agtagtaata 189360

attttaatgt ttaacgccta cagcagtagt aat 189393

Claims

1. The application of preparing the attenuated African swine fever virus strain or African swine fever vaccine by jointly losing the functions of the protein coded by the ASFV G-ACD-00190 gene, the ASFV MGF-110-9L gene, the ASFV G-ACD-00210 gene and the ASFV MGF-505-7R gene in the gene II type African swine fever virus.

2. The application of preparing the attenuated African swine fever virus strain or the Zhou swine fever vaccine by jointly deleting the gene fragment in the gene II African swine fever virus is characterized in that the gene fragment comprises all or part of the nucleotide sequence of the ASFV G-ACD-00190 gene, all or part of the nucleotide sequence of the ASFV MGF-110-9L gene, all or part of the nucleotide sequence of the ASFV G-ACD-00210 gene and all or part of the nucleotide sequence of the ASFV MGF-505-7R gene.

3. The use according to claim 1 or 2, the gene II type African swine fever virus comprises any one of ASFV CN/GS/2018 isolates, China/2018/AnhuiXCGQ, Pig/CN/HLJ/2018, Georgia2007, ASFV-SY18, ASFV/POL/2015/Podlaskie, ASFV/POL17_05838_ C220, ASFV/POL16_29413-o23, ASFV/POL16_20538_ o9, ASFV/POL17_04461_ C210, ASFV/POL16_20186_ o7, ASFV/POL17_03029_ C201, ASFV/POL16_20540_ o10, ASFV Czechublic 2017/1, ASFV Molda 2017/1, ASFV Begilum 2018/1, ASFV German 2020/1, ASFV/ARFV/CBFV/2018/CAS 2019, ASFV _ 468/China 468, ASFV/CAS 3, ASFV _ 468 _ CBFV _ 2008 _ GCH 3; the whole gene sequence of the ASFV CN/GS/2018 isolate is shown in SEQ ID NO. 25.

4. The use as claimed in claim 3, wherein the deleted gene fragment is the full-length sequence of the ASFV CN/GS/2018 isolate at positions 11627-12499 and 40752-42335.

5. A gene deletion attenuated African swine fever virus strain is a gene II type African swine virus with a gene fragment deletion, wherein the gene fragment comprises all or part of nucleotide sequence of ASFV G-ACD-00190 gene, all or part of nucleotide sequence of ASFV MGF-110-9L gene, all or part of nucleotide sequence of ASFV G-ACD-00210 gene and all or part of nucleotide sequence of ASFV MGF-505-7R gene.

6. The gene deletion attenuated African swine fever virus strain of claim 5, the gene II type African swine fever virus comprises any one of ASFV CN/GS/2018 isolates, China/2018/AnhuiXCGQ, Pig/CN/HLJ/2018, Georgia2007, ASFV-SY18, ASFV/POL/2015/Podlaskie, ASFV/POL17_05838_ C220, ASFV/POL16_29413-o23, ASFV/POL16_20538_ o9, ASFV/POL17_04461_ C210, ASFV/POL16_20186_ o7, ASFV/POL 2 _03029_ C201, ASFV/POL16_20540_ o10, ASFV Czechubblic 2017/1, ASFV Moldua 2017/1, ASFV Belgium 2018/1, ASFV German/German 6, ASFV/2/ASFV _ 19, ASFV/HSV 358 _ 368, ASFV _ 368 _ CBFV _ 369/CAS 3, ASFV _ 368/ASFV _ 368; the whole gene sequence of the ASFV CN/GS/2018 isolate is shown in SEQ ID NO. 25.

7. The gene deletion attenuated African swine fever virus strain of claim 6, wherein the deleted gene fragment is the 11627-12499 and 40752-42335 position of the full length sequence of the ASFV CN/GS/2018 isolate.

8. An attenuated vaccine against African swine fever comprising the attenuated African swine fever virus strain having the gene deletion of any one of claims 5 to 7.

9. A process for preparing an attenuated African swine fever virus strain with a gene deletion as claimed in any one of claims 5 to 7, which comprises deleting all or part of the nucleotide sequence of the ASFV G-ACD-00190 gene, all or part of the nucleotide sequence of the ASFV MGF-110-9L gene, all or part of the nucleotide sequence of the ASFV G-ACD-00210 gene and all or part of the nucleotide sequence of the ASFV MGF-505-7R gene from the original African swine fever virus type II by genetic engineering means.

10. A method for preparing an attenuated african swine fever virus strain with a gene deletion according to claim 7, comprising the steps of:

(1) designing 1.5kb of upstream and downstream sequences of ASFV MGF-505-7R gene as left and right homologous recombination arms, cloning the left and right homologous recombination arm genes and eGFP gene screening expression cassette gene fragment p72-eGFP-SV40polyA to a pUC57 vector at the same time, and obtaining a recombinant plasmid p 53-eGFP;

(2) designing 1.5kb of upstream and downstream sequences of ASFV MGF-110-9L gene respectively as left and right homologous recombination arms, and cloning the left and right homologous recombination arms and mCherry gene screening expression cassette gene fragment p72-mCherry-NOSpolyA to a pUC57 vector at the same time to obtain recombinant plasmid p 20-mCherry;

(3) transfecting recombinant plasmids p53-eGFP and p20-mCherry to BMDM cells infected with ASFV original strains, and purifying and screening by adopting a plaque cloning method to obtain an attenuated African swine fever virus delta 20/53 with combined deletion of ASFV G-ACD-00190 gene, ASFV MGF-110-9L gene, ASFV G-ACD-00210 gene and ASFV MGF-505-7R gene.