CN113214963A - Escherichia coli activation fermentation fire fighting equipment - Google Patents
Escherichia coli activation fermentation fire fighting equipment Download PDFInfo
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- CN113214963A CN113214963A CN202110536827.4A CN202110536827A CN113214963A CN 113214963 A CN113214963 A CN 113214963A CN 202110536827 A CN202110536827 A CN 202110536827A CN 113214963 A CN113214963 A CN 113214963A
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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- C12M29/20—Degassing; Venting; Bubble traps
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/02—Means for regulation, monitoring, measurement or control, e.g. flow regulation of foam
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Abstract
The invention relates to the technical field of fermentation, and discloses an escherichia coli activating, fermenting and defoaming device which comprises a fermentation tank, wherein a discharge hole is formed in the bottom of the fermentation tank, a feed inlet is formed in one side, away from the discharge hole, of the fermentation tank, a piston plate is connected inside the fermentation tank in a sliding mode, the piston plate is fixedly connected with a sliding rod, one end, close to the discharge hole, of the sliding rod is connected with a sliding sleeve in a sliding mode, a fixed seat is arranged at the end part of the sliding sleeve, one side, away from the sliding sleeve, of the fixed seat is connected with a filter plate, one side, close to the sliding sleeve, of the piston plate is rotatably connected with one end of a first supporting rod, the other end of the first supporting rod is rotatably connected with a connecting seat, one side, close to the inner wall of the fermentation tank, of the connecting seat is rotatably connected with one end of a second supporting rod, and the other end of the second supporting rod is rotatably connected with the inside of the fermentation tank. The invention is suitable for an escherichia coli activation fermentation defoaming device, and the filter plate can move upwards at a higher speed, so that bubbles in the filter plate are discharged to realize defoaming treatment.
Description
Technical Field
The invention relates to the technical field of fermentation, in particular to an escherichia coli activation fermentation defoaming device.
Background
Escherichia coli, also called Escherichia coli, is a conditional pathogen, and can cause gastrointestinal tract infection or urinary tract infection of various local tissues and organs of human and various animals under certain conditions.
Fermentation engineering is an important component of biotechnology and an important link of biotechnology industrialization. The fermentation technology has a long history, and the microbial fermentation industry as a modern scientific concept combines modern new technologies of genetic engineering, cell engineering and the like on the basis of the traditional fermentation technology and is often accompanied with the expression of protein in the fermentation process. In the microbial fermentation process, strain activation is a prerequisite for ensuring the fermentation stability and also influences the expression of target protein, and is a crucial link. Amino acid decarboxylase, when it is catalyzed, can decompose amino acid and decarboxylate it to produce amine and carbon dioxide, thereby producing foam.
During the process of activating and fermenting the escherichia coli, aerobic respiration and anaerobic respiration can be carried out. In general, the cultivation of Escherichia coli requires aeration for oxygen supply. Air bubbles are generated when culturing escherichia coli in a fermenter which is aerated and supplied with oxygen, and after the cultivation of escherichia coli is finished, the treatment of escherichia coli bacterial liquid is required to be continued, and air bubbles or foams may be generated in the escherichia coli bacterial liquid. Therefore, a device is needed to remove the bubbles or foams.
Disclosure of Invention
The invention provides an escherichia coli activation fermentation defoaming device, which solves the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme:
an escherichia coli activating, fermenting and defoaming device comprises a fermentation tank, a discharge outlet is arranged at the bottom of the fermentation tank, a feed inlet is arranged on one side of the fermentation tank far away from the discharge outlet, a piston plate is connected inside the fermentation tank in a sliding manner, the piston plate is fixedly connected with a sliding rod, one end of the sliding rod close to the discharge outlet is connected with a sliding sleeve in a sliding manner, a fixed seat is arranged at the end part of the sliding sleeve, one side of the fixed seat far away from the sliding sleeve is connected with a filter plate, one side of the piston plate close to the sliding sleeve is rotatably connected with one end of a first supporting rod, the other end of the first supporting rod is rotatably connected with a connecting seat, one side of the connecting seat close to the inner wall of the fermentation tank is rotatably connected with one end of a second supporting rod, the other end of the second supporting rod is rotatably connected with the inside of the fermentation tank, one side of the connecting seat close to the sliding rod is rotatably connected with one end of a third supporting rod, and the other end of the third supporting rod is rotatably connected with a sliding sleeve.
As a preferable technical solution of the present invention, a first spring is provided between the sliding rod and the sliding sleeve.
As a preferable technical scheme of the invention, one side of the sliding rod, which is far away from the sliding sleeve, is connected with the end part of the fermentation tank in a sliding way.
As a preferable technical scheme of the invention, one end of the sliding rod, which is far away from the sliding sleeve, is connected with a fixed rod, and the end part of the fixed rod is connected with a piston rod of the air cylinder.
As a preferred technical scheme of the invention, a piston rod of the air cylinder is provided with a connector, the connector is fixedly connected with the end part of the fixed rod, and the connector is L-shaped.
As a preferable technical scheme of the invention, one side of the fixed rod, which is far away from the air cylinder, is rotatably connected with one end of a fourth supporting rod, the other end of the fourth supporting rod is rotatably connected with a sliding block, the sliding block is slidably connected with a guide rod, one side of the guide rod, which is close to the fermentation tank, is fixedly connected with a cover plate, and one end of the cover plate is rotatably connected with an air outlet formed in the side wall of the fermentation tank.
As a preferable technical scheme of the invention, the end part of the guide rod is provided with a limiting block, and a second spring is arranged between the limiting block and the sliding block.
As a preferred technical scheme of the invention, a sealing ring is arranged between the piston plate and the inner wall of the fermentation tank.
As a preferred technical scheme of the invention, the bottom outer side of the fermentation tank is provided with a supporting leg, and the end part of the supporting leg is provided with a base plate.
As a preferred technical scheme of the invention, the bottom of the fermentation tank is provided with a guide plate which is conical.
The invention has the following advantages:
the invention is suitable for an escherichia coli activating, fermenting and defoaming device, and through the mutual rotation of the first support rod, the second support rod and the third support rod, so that the filter plate can move downward at a faster speed when the slide bar moves downward, and in the same way, the filter plate can move upward at a faster speed when the slide bar moves upward, thereby discharging bubbles in the filter plate to realize defoaming treatment, and the air pressure in the fermentation tank can be automatically adjusted due to the up-and-down movement of the piston plate, in the defoaming process, the air pressure is reduced, which is beneficial to the gas to form bubbles to be discharged, and through the action of the fourth support rod, when the piston plate descends to carry out fermentation treatment, the exhaust port can be blocked by the cover plate, the sealing effect of the device is improved, when the piston plate rises and needs to be exhausted, the cover plate is opened, so that the automation degree of the device is higher.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic structural diagram of an apparatus for activating, fermenting and defoaming Escherichia coli.
FIG. 2 is a front view of an apparatus for activating fermentation and defoaming Escherichia coli.
FIG. 3 is a sectional view of a fermentation tank in an apparatus for activating, fermenting and defoaming Escherichia coli.
Fig. 4 is a front view of fig. 3.
Fig. 5 is a partially enlarged view of a in fig. 3.
FIG. 6 is a schematic view of the structure of the connection between the sliding sleeve and the sliding rod in the defoaming device for Escherichia coli activating fermentation.
In the figure: 1. a base plate; 2. supporting legs; 3. a fermentation tank; 4. a feed inlet; 5. an exhaust port; 6. a cylinder; 7. a connector; 8. fixing the rod; 9. a slide bar; 10. a discharge outlet; 11. a piston plate; 12. a seal ring; 13. a first support bar; 14. a connecting seat; 15. a second support bar; 16. a third support bar; 17. a first spring; 18. a sliding sleeve; 19. a fixed seat; 20. a filter plate; 21. a through hole; 22. a fourth support bar; 23. a cover plate; 24. a guide bar; 25. a slider; 26. a second spring; 27. a limiting block; 28. a baffle.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In one embodiment, referring to fig. 1-6, an apparatus for activating, fermenting and defoaming escherichia coli comprises a fermentation tank 3, wherein a support leg 2 is arranged at the outer side of the bottom of the fermentation tank 3, and a pad 1 is arranged at the end of the support leg 2. The bottom of fermentation cylinder 3 is equipped with bin outlet 10, one side that fermentation cylinder 3 kept away from bin outlet 10 is equipped with feed inlet 4, 3 inside sliding connection piston plates 11 of fermentation cylinder, be equipped with sealing washer 12 between piston plate 11 and the 3 inner walls of fermentation cylinder. The air tightness between the piston plate 11 and the fermentation tank 3 is better by arranging the sealing ring 12, and the subsequent pressurizing operation is guaranteed. The piston plate 11 is fixedly connected with a sliding rod 9, one end of the sliding rod 9 close to the discharge opening 10 is slidably connected with a sliding sleeve 18, and a first spring 17 is arranged between the sliding rod 9 and the sliding sleeve 18. By arranging the first spring 17, the sliding sleeve 18 is connected with the sliding rod 9 more stably without shaking. The side of the sliding rod 9 far away from the sliding sleeve 18 is connected with the end part of the fermentation tank 3 in a sliding way.
In one aspect of this embodiment, referring to fig. 3-5, the end of the sliding sleeve 18 is provided with a fixing base 19, a side of the fixing base 19 away from the sliding sleeve 18 is connected to a filter plate 20, the filter plate 20 is provided with a through hole 21 inside, during the up and down movement of the filter plate 20, the large volume of bubbles is separated from the raw material, thereby pushing the bubbles upwards to be discharged out of the device, one side of the piston plate 11 close to the sliding sleeve 18 is rotatably connected with one end of a first supporting rod 13, the other end of the first supporting rod 13 is rotatably connected with a connecting seat 14, one side of the connecting seat 14 close to the inner wall of the fermentation tank 3 is rotatably connected with one end of a second supporting rod 15, the other end of the second supporting rod 15 is rotatably connected with the inside of the fermentation tank 3, one side of the connecting seat 14 close to the sliding rod 9 is rotatably connected with one end of a third supporting rod 16, and the other end of the third supporting rod 16 is rotatably connected with a sliding sleeve 18.
In one aspect of this embodiment, referring to fig. 3, an end of the sliding rod 9 away from the sliding sleeve 18 is connected to the fixing rod 8, and an end of the fixing rod 8 is connected to a piston rod of the cylinder 6. The piston rod of cylinder 6 is equipped with connector 7, and the tip of connector 7 fixed connection dead lever 8, connector 7 are the L shape. Can drive dead lever 8 through setting up cylinder 6 and reciprocate to because the interval between dead lever 8 and the fermentation cylinder 3 is less, consequently the connector 7 through L shape is connected the piston rod of cylinder 6 with dead lever 8, has realized that cylinder 6 drives the effect that dead lever 8 reciprocated.
In one aspect of this embodiment, referring to fig. 5, one side of the fixing rod 8 away from the cylinder 6 is rotatably connected to one end of a fourth supporting rod 22, the other end of the fourth supporting rod 22 is rotatably connected to a sliding block 25, the sliding block 25 is slidably connected to a guiding rod 24, one side of the guiding rod 24 close to the fermentation tank 3 is fixedly connected to a cover plate 23, and one end of the cover plate 23 is rotatably connected to an exhaust port 5 disposed on a side wall of the fermentation tank 3. The end of the guide rod 24 is provided with a limit block 27, and a second spring 26 is arranged between the limit block 27 and the sliding block 25.
In one aspect of this embodiment, referring to fig. 4, a baffle 28 is disposed at the bottom of the fermentation tank 3, and the baffle 28 is conical. The discharge opening 10 is arranged at the center of the guide plate 28, so that the discharge processing is facilitated. In order to facilitate the discharge of the materials inside the fermentation tank 3, the bottom of the fermentation tank 3 can be provided with a conical guide plate 28, and the materials flow on the conical surface, so that the materials inside the fermentation tank 3 can be discharged, and the accumulation at the bottom of the fermentation tank 3 is avoided.
In the implementation process of the embodiment, when the device is used, the air cylinder 6 is firstly started, the piston rod of the air cylinder 6 extends out, so that the fixed rod 8 moves upwards, the piston plate 11 moves upwards at the moment, when the piston plate 11 moves to the topmost part of the device, the feed inlet 4 is opened at the moment, the feed inlet 4 is connected with the conveying device of raw materials, or corresponding raw materials for activating and fermenting escherichia coli are directly added into the fermentation tank 3 through the feed inlet 4, after the addition is completed, the air cylinder 6 can be reversely started, the piston rod of the air cylinder 6 contracts, so that the fixed rod 8 moves downwards, the fixed rod 8 pushes the cover plate 23 to rotate clockwise through the fourth support rod 22, so that the exhaust port 5 is closed, the fixed rod 8 pushes the piston plate 11 to move downwards through the sliding rod 9, when the piston plate 11 is lower than the feed inlet 4, the device is in a closed state at the moment, and as the piston plate 11 moves downwards, the first support rod 13 and the second support rod 15 rotate with each other, so that the connecting seat 14 moves towards the direction of the sliding rod 9, and the third support rod 16 pushes the sliding sleeve 18 to move downwards, so that the filter plate 20 moves downwards, and after the filter plate 20 moves to the bottommost part of the device, the device is in a ready state, and escherichia coli can perform activation fermentation.
After the activated fermentation is carried out for a period of time, a certain amount of gas is generated by respiration, the cylinder 6 can be started reversely at the moment, the piston rod of the cylinder 6 extends out, so that the piston plate 11 moves upwards, the pressure in the fermentation tank 3 is gradually reduced at the moment, the generated gas forms bubbles to slowly move upwards, meanwhile, the second support rod 15 and the first support rod 13 rotate reversely, so that the connecting seat 14 moves towards the direction far away from the sliding rod 9, so that the sliding sleeve 18 moves upwards relative to the sliding rod 9, so that the filter plate 20 pushes the generated bubbles upwards, in the moving process, the fixing rod 8 pulls the cover plate 23 to rotate anticlockwise through the fourth support rod 22, so that the exhaust port 5 is opened, after the piston plate 11 moves to the upper part of the exhaust port 5, the air pressure in the fermentation tank 3 rapidly drops, and the generated gas forms bubbles rapidly, and moves to the upper part of the apparatus by its own buoyancy and the filter plate 20 and is discharged through the exhaust port 5, realizing a defoaming treatment.
The invention is suitable for a colibacillus activation fermentation defoaming device, through setting up the mutual rotation of the first brace rod 13, the second brace rod 15 and the third brace rod 16, make when the slide bar 9 moves downwards, the filter plate 20 can move downwards with the faster speed, when the slide bar 9 moves upwards similarly, the filter plate 20 can move upwards with the faster speed, thus discharge the bubble in the filter plate 20 and realize the treatment of defoaming, and because the up-and-down movement of the piston plate 11, make the air pressure in the fermentation cylinder 3 regulate by oneself, in the course of defoaming, reduce the air pressure, help the gas to form the bubble to discharge, and also through the function of the fourth brace rod 22, make the piston plate 11 descend and ferment and process, the cover plate 23 can block the exhaust port 5, has improved the sealed effect of the device, when the piston plate 11 rises and needs to exhaust, the cover 23 is opened, so that the automation of the device is higher.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Claims (10)
1. An escherichia coli activating, fermenting and defoaming device comprises a fermentation tank, a discharge hole is arranged at the bottom of the fermentation tank, a feed inlet is arranged at one side of the fermentation tank far away from the discharge hole, it is characterized in that the interior of the fermentation tank is connected with a piston plate in a sliding way, the piston plate is fixedly connected with a sliding rod, one end of the sliding rod close to the discharge port is connected with a sliding sleeve in a sliding way, the end part of the sliding sleeve is provided with a fixed seat, one side of the fixed seat far away from the sliding sleeve is connected with a filter plate, one side of the piston plate close to the sliding sleeve is rotatably connected with one end of a first supporting rod, the other end of the first supporting rod is rotatably connected with a connecting seat, one side of the connecting seat close to the inner wall of the fermentation tank is rotatably connected with one end of a second supporting rod, the other end of the second supporting rod is rotatably connected with the inside of the fermentation tank, one side of the connecting seat close to the sliding rod is rotatably connected with one end of a third supporting rod, and the other end of the third supporting rod is rotatably connected with a sliding sleeve.
2. The device for activating, fermenting and defoaming escherichia coli as recited in claim 1, wherein a first spring is arranged between the sliding rod and the sliding sleeve.
3. The apparatus for activating, fermenting and defoaming Escherichia coli as claimed in claim 1, wherein the side of said sliding rod away from the sliding sleeve is slidably connected to the end of the fermentation tank.
4. The escherichia coli activation, fermentation and defoaming device as claimed in claim 1, wherein one end of the sliding rod, which is far away from the sliding sleeve, is connected with a fixed rod, and the end of the fixed rod is connected with a piston rod of a cylinder.
5. The Escherichia coli activating, fermenting and defoaming device as claimed in claim 4, wherein the piston rod of the cylinder is provided with a connector, the connector is fixedly connected with the end of the fixing rod, and the connector is L-shaped.
6. The device for activating, fermenting and defoaming escherichia coli as claimed in claim 4, wherein one side of the fixing rod away from the cylinder is rotatably connected with one end of a fourth supporting rod, the other end of the fourth supporting rod is rotatably connected with a sliding block, the sliding block is slidably connected with a guide rod, one side of the guide rod close to the fermentation tank is fixedly connected with a cover plate, and one end of the cover plate is rotatably connected with an exhaust port arranged on the side wall of the fermentation tank.
7. The escherichia coli activation, fermentation and defoaming device as claimed in claim 6, wherein a limiting block is arranged at an end of the guide rod, and a second spring is arranged between the limiting block and the sliding block.
8. The device for activating, fermenting and defoaming escherichia coli as claimed in claim 1, wherein a sealing ring is arranged between the piston plate and the inner wall of the fermentation tank.
9. The Escherichia coli activating, fermenting and defoaming device as claimed in claim 1, wherein the bottom of the fermentation tank is provided with support legs at the outer side, and the ends of the support legs are provided with backing plates.
10. The escherichia coli activation, fermentation and defoaming device as claimed in claim 1, wherein a guide plate is arranged at the bottom of the fermentation tank, and the guide plate is conical.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110536827.4A CN113214963B (en) | 2021-05-17 | 2021-05-17 | Escherichia coli activation fermentation fire fighting equipment |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110536827.4A CN113214963B (en) | 2021-05-17 | 2021-05-17 | Escherichia coli activation fermentation fire fighting equipment |
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| CN113214963A true CN113214963A (en) | 2021-08-06 |
| CN113214963B CN113214963B (en) | 2022-01-11 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN118126813A (en) * | 2024-05-08 | 2024-06-04 | 淄博市动物疫病预防与控制中心 | Escherichia coli activation fermentation treatment equipment |
| CN118374340A (en) * | 2024-06-20 | 2024-07-23 | 青岛市动物疫病预防控制中心 | Escherichia coli fermentation equipment |
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| KR20160056105A (en) * | 2014-11-11 | 2016-05-19 | 주식회사 월드바이오텍 | Fermenter having part for removing bubbles |
| CN205295329U (en) * | 2016-01-11 | 2016-06-08 | 山东鲁抗舍里乐药业有限公司 | Biological fermentation defoaming and feed supplement device |
| CN208701058U (en) * | 2018-08-17 | 2019-04-05 | 云南亚联科技有限公司 | Fermentor with defoaming structure |
| CN208776724U (en) * | 2018-08-24 | 2019-04-23 | 厦门华厦学院 | Microbial fermentation Quick defoaming device |
| CN210394363U (en) * | 2019-07-01 | 2020-04-24 | 安徽科宝生物工程有限公司 | Escherichia coli high density fermentation device |
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2021
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20160056105A (en) * | 2014-11-11 | 2016-05-19 | 주식회사 월드바이오텍 | Fermenter having part for removing bubbles |
| CN205295329U (en) * | 2016-01-11 | 2016-06-08 | 山东鲁抗舍里乐药业有限公司 | Biological fermentation defoaming and feed supplement device |
| CN208701058U (en) * | 2018-08-17 | 2019-04-05 | 云南亚联科技有限公司 | Fermentor with defoaming structure |
| CN208776724U (en) * | 2018-08-24 | 2019-04-23 | 厦门华厦学院 | Microbial fermentation Quick defoaming device |
| CN210394363U (en) * | 2019-07-01 | 2020-04-24 | 安徽科宝生物工程有限公司 | Escherichia coli high density fermentation device |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN118126813A (en) * | 2024-05-08 | 2024-06-04 | 淄博市动物疫病预防与控制中心 | Escherichia coli activation fermentation treatment equipment |
| CN118374340A (en) * | 2024-06-20 | 2024-07-23 | 青岛市动物疫病预防控制中心 | Escherichia coli fermentation equipment |
| CN118374340B (en) * | 2024-06-20 | 2024-08-20 | 青岛市动物疫病预防控制中心 | Escherichia coli fermentation equipment |
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| CN113214963B (en) | 2022-01-11 |
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