CN113201454A - Small-size nucleic acid rapid annealing device using liquid exchange method - Google Patents
Small-size nucleic acid rapid annealing device using liquid exchange method Download PDFInfo
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- CN113201454A CN113201454A CN202110573132.3A CN202110573132A CN113201454A CN 113201454 A CN113201454 A CN 113201454A CN 202110573132 A CN202110573132 A CN 202110573132A CN 113201454 A CN113201454 A CN 113201454A
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Abstract
The invention relates to a small-sized nucleic acid rapid annealing device using a liquid exchange method, belonging to the technical field of nucleic acid detection. The rapid annealing device comprises a shell, a control module, a control knob, a liquid exchange device and a heating tank, wherein the control module is positioned above the shell; the heating tank and the liquid exchange device are positioned in the shell, the shell is provided with a power interface, and the power interface is communicated with the control module; the heating device is characterized in that a test tube rack is arranged in the heating groove, liquid heat transfer media are contained in the heating groove and the liquid exchange device, a heating module is arranged between the test tube rack and a bottom plate of the heating groove, and the heating module and the liquid exchange device are respectively communicated with the control module. The invention can rapidly anneal and cool, uses the liquid exchange device as a cooling device, ensures the volume of the instrument, simultaneously realizes the accurate control of the temperature, has simple operation, does not contact the heat transfer medium with the solution in the test tube, is not easy to cause secondary pollution to the experiment, and ensures the accuracy of the experiment.
Description
Technical Field
The invention relates to a small-sized nucleic acid rapid annealing device using a liquid exchange method, belonging to the technical field of nucleic acid detection.
Background
Nucleic acid detection refers to a technique for detecting whether a sample contains a target nucleic acid sequence using a fluorescent probe. When nucleic acid detection is performed, nucleic acid annealing is required. Nucleic acid annealing refers to the process of DNA molecule denaturation by heat, double strand separation, and slow cooling to a suitable temperature for DNA renaturation.
Two of the most common annealing instruments in laboratories today are water baths and PCR instruments. The water bath pot adopts numerical control display at present, is simple to operate, but has large instrument volume, occupies the space of an operation table, is slow in temperature rise and fall, and cannot be suitable for experiments with quick related chemical reactions and strict temperature requirements. Only nucleic acid annealing is required to be performed using a PCR instrument, and PCR extension and denaturation are not required. The PCR instrument is essentially a temperature control device and can be well controlled among denaturation temperature, renaturation temperature and extension temperature. The PCR instrument has the advantages of accurate temperature control, large range, high speed, and high price and operation difficulty. There are also small temperature control devices that use semiconductor heating modules in keeping with the PCR principle.
Therefore, the water bath and the PCR instrument respectively have the defects of long time consumption, complex operation, high price and the like, and the rapid annealing device suitable for the nucleic acid detection method needs to be researched and developed urgently.
Disclosure of Invention
[ problem ] to
The annealing device of the existing nucleic acid detection method has the disadvantages of overlong cooling time, poor constant temperature, large device volume, complex operation, time and labor consumption, slow cooling in the annealing process and long annealing process.
[ solution ]
The invention provides a small-sized nucleic acid rapid annealing device using a liquid exchange method, which is suitable for the small-sized nucleic acid rapid annealing of a nucleic acid sensor, is convenient and fast to operate, can rapidly anneal and cool, and can accurately control the temperature.
A small-sized nucleic acid rapid annealing device applying a liquid exchange method comprises a shell, a control module, a control knob, a liquid exchange device and a heating tank, wherein the control module is positioned above the shell; the heating tank and the liquid exchange device are positioned in the shell, the shell is provided with a power interface, and the power interface is communicated with the control module; the heating device is characterized in that a test tube rack is arranged in the heating groove, liquid heat transfer media are contained in the heating groove and the liquid exchange device, a heating module is arranged between the test tube rack and a bottom plate of the heating groove, and the heating module and the liquid exchange device are respectively communicated with the control module.
In one embodiment of the present invention, a removable cover plate is disposed above the heating tank.
In one embodiment of the invention, the liquid heat transfer medium is water.
In one embodiment of the invention, the housing side wall is provided with a vent hole.
In one embodiment of the invention, the vent is a louver.
In one embodiment of the present invention, the heating module is a heating wire or a heating sheet.
In one embodiment of the invention, the control module is provided with a prompt alarm lamp.
In one embodiment of the present invention, the liquid exchange device includes a first water tank, a second water tank, a third water tank, a first control valve, a second control valve, a third control valve, and an electric pump, the first control valve is disposed between the first water tank and the second water tank, the electric pump is disposed in the second water tank, the third control valve is disposed between the second water tank and the third water tank, and the second control valve is disposed between the third water tank and the first water tank.
In one embodiment of the present invention, the test tube rack is located in the first water tank.
In one embodiment of the present invention, a control knob is disposed above the control module.
[ advantageous effects ]
The invention discloses a small-sized nucleic acid rapid annealing device using a liquid exchange method, which uses a liquid exchange device to carry out cold-heat conversion of liquid, realizes rapid cooling and simultaneously ensures a relatively stable environment of renaturation. The operation is convenient, and the medium that can rapid annealing cooling use water or other liquid as the heat transfer has realized the accurate control to the temperature when having guaranteed the instrument volume. The heat transfer medium is not contacted with the solution in the test tube, so that the secondary pollution to the experiment is not easy to cause, the accuracy of the experiment is ensured, and the device is more suitable for teaching and use in the class of the general texthouse and meets the requirements of students on entering into relevant laboratory learning practice operation knowledge and the like.
Drawings
FIG. 1 is a perspective view of a rapid annealing apparatus according to the present invention;
FIG. 2 is a schematic view of the construction of the liquid exchange apparatus of the present invention;
in FIGS. 1-2, 1-housing; 2-a control module; 3-a control knob; 4-a removable cover plate; 5-test tube rack; 6-a liquid exchange device; 7-heating the module; 8-a vent hole; 9-heating a groove; 10-a power interface; 11-a first water tank; 12-a second water tank; 13-a third water tank; 14-a first control valve; 15-a second control valve; 16-a third control valve; 17-electric pump.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to specific embodiments and the accompanying drawings.
Example 1
As shown in FIG. 1, a small-sized nucleic acid rapid annealing device using a liquid exchange method comprises a housing 1, a control module 2, a control knob 3, a liquid exchange device 6 and a heating tank 9, wherein the control module 2 is positioned above the housing 1; the heating tank 9 and the liquid exchange device 6 are positioned inside the shell 1, the shell 1 is provided with a power interface 10, and the power interface 10 is communicated with the control module 2; the test tube rack is characterized in that a test tube rack 5 is arranged in the heating groove 9, liquid heat transfer media are contained in the heating groove 9 and the liquid exchange device 6, a heating module 7 is arranged between the test tube rack 5 and a bottom plate of the heating groove 9, and the heating module 7 and the liquid exchange device 6 are respectively communicated with the control module 2.
Preferably, the upper part of the heating groove 9 is also provided with a movable cover plate 4. The liquid heat transfer medium is water. The side wall of the shell 1 is also provided with a vent hole 8 in a shutter mode, and the number of the holes can be increased or decreased according to specific conditions. The heating module 7 is an electric heating wire or an electric heating sheet.
Preferably, the liquid exchanging device 6 includes a first water tank 11, a second water tank 12, a third water tank 13, a first control valve 14, a second control valve 15, a third control valve 16 and an electric pump 17, the first control valve 14 is disposed between the first water tank 11 and the second water tank 12, the electric pump 17 is disposed in the second water tank 12, the third control valve 16 is disposed between the second water tank 12 and the third water tank 13, and the second control valve 15 is disposed between the third water tank 13 and the first water tank 11.
When the small-sized nucleic acid rapid annealing device using the liquid exchange method is in the unwinding stage, the heating process is performed in the first water tank 11, the third control valve 16 is opened, the liquid in the second water tank 12 flows into the third water tank 13 by using the electric pump 17, and the third control valve 16 is closed; when the small-sized nucleic acid rapid annealing device using the liquid exchange method is in the annealing stage, the first water tank 11 is heated, the first control valve 14 is opened to make the hot liquid in the first water tank 11 enter the second water tank 12, and the second control valve 15 is opened to make the cold liquid in the third water tank 13 flow into the first water tank 11, and the proper temperature is set for heating. After the annealing is finished, the first control valve 14 and the second control valve 15 are closed, and the rapid annealing device is returned to the initial state.
Preferably, the test tube rack 5 is located in the first water tank 11.
Preferably, the control knob 3 is controlled by a knob, and an operator can directly rotate the knob to start the set heating program of the device.
Preferably, control module 2 is equipped with the suggestion alarm lamp, has the warning function, and when once annealing operation was accomplished, control knob 3 resets, and control module 2 can send and drip warning sound and warning flashing light.
Preferably, the power interface 10 is located on the housing 1 and can be externally connected with a household 220V power supply.
Preferably, the heating module 7 is arranged below the test tube rack 5, is started by the control module 2 and the control knob 3, is powered by the power interface 10, and indirectly realizes heating through a liquid environment.
Preferably, the liquid exchange device 6 is arranged on the side surface of the heating tank 9, is started by the control module 2 and the control knob 3, is powered by the power interface 10, and realizes the cooling effect through liquid exchange.
When nucleic acid annealing is required, a test tube containing a sample is inserted into the test tube rack, fixed to the test tube rack 5, and the movable cover plate 4 is closed. The control knob 3 is started and then operated, the control knob 3 is reset after the annealing operation is finished, and the prompt tone is transmitted out from the control module 2 to take out a sample.
The invention discloses a small-sized nucleic acid rapid annealing device using a liquid exchange method, which uses a liquid exchange device to carry out cold-heat conversion of liquid, realizes rapid cooling and simultaneously ensures a relatively stable environment of renaturation. The operation is convenient, and the medium that can rapid annealing cooling use water or other liquid as the heat transfer has realized the accurate control to the temperature when having guaranteed the instrument volume. The heat transfer medium is not contacted with the solution in the test tube, so that the secondary pollution to the experiment is not easy to cause, the accuracy of the experiment is ensured, and the device is more suitable for teaching and use in the class of the general texthouse and meets the requirements of students on entering into relevant laboratory learning practice operation knowledge and the like.
Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes in the embodiments and/or modifications of the invention can be made, and equivalents may be substituted for elements thereof without departing from the scope of the invention.
Claims (10)
1. A small-sized nucleic acid rapid annealing device using a liquid exchange method is characterized by comprising a shell (1), a control module (2), a control knob (3), a liquid exchange device (6) and a heating groove (9), wherein the control module (2) is positioned above the shell (1); the heating tank (9) and the liquid exchange device (6) are positioned in the shell (1), the shell (1) is provided with a power interface (10), and the power interface (10) is communicated with the control module (2); be equipped with test-tube rack (5) in heating tank (9), contain liquid heat-transfer medium in heating tank (9) and liquid exchange device (6), be equipped with heating module (7) between the bottom plate of test-tube rack (5) and heating tank (9), heating module (7) and liquid exchange device (6) switch-on with control module (2) respectively.
2. The rapid annealing apparatus for small nucleic acid using liquid exchange method according to claim 1, wherein a removable cover plate (4) is provided above the heating tank (9).
3. The apparatus for rapid annealing of small nucleic acid using liquid exchange method according to claim 2, wherein the liquid heat transfer medium is water.
4. The rapid annealing device for small nucleic acid using liquid exchange method according to claim 3, wherein the side wall of the housing (1) is provided with a vent (8).
5. The rapid annealing device for small nucleic acid using liquid exchange method according to claim 4, wherein said ventilation holes (8) are louvers.
6. The rapid annealing device for small nucleic acid using liquid exchange method according to claim 5, wherein the heating module (7) is a heating wire or a heating plate.
7. The rapid annealing device for small nucleic acid using liquid exchange method according to claim 6, wherein the control module (2) is provided with a warning light.
8. The rapid annealing device for small nucleic acids using liquid exchange method according to claim 7, wherein the liquid exchange device (6) comprises a first water tank (11), a second water tank (12), a third water tank (13), a first control valve (14), a second control valve (15), a third control valve (16) and an electric pump (17), the first control valve (14) is disposed between the first water tank (11) and the second water tank (12), the electric pump (17) is disposed between the second water tank (12), the third control valve (16) is disposed between the second water tank (12) and the third water tank (13), and the second control valve (15) is disposed between the third water tank (13) and the first water tank (11).
9. The rapid annealing device for small nucleic acid using liquid exchange method according to claim 8, wherein the test tube rack (5) is located in the first water tank (11).
10. The rapid annealing device for small nucleic acid using liquid exchange method according to any one of claims 1-9, wherein a control knob (3) is provided above the control module (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110573132.3A CN113201454A (en) | 2021-05-25 | 2021-05-25 | Small-size nucleic acid rapid annealing device using liquid exchange method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110573132.3A CN113201454A (en) | 2021-05-25 | 2021-05-25 | Small-size nucleic acid rapid annealing device using liquid exchange method |
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| CN113201454A true CN113201454A (en) | 2021-08-03 |
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| CN202110573132.3A Pending CN113201454A (en) | 2021-05-25 | 2021-05-25 | Small-size nucleic acid rapid annealing device using liquid exchange method |
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Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19852733A1 (en) * | 1998-11-16 | 2000-05-25 | Max Planck Gesellschaft | Method and device for tempering a liquid bath |
| CN101565672A (en) * | 2008-04-21 | 2009-10-28 | 张永钢 | Liquid circulating gene amplification equipment |
| CN102046291A (en) * | 2008-04-04 | 2011-05-04 | It-Is国际有限公司 | Thermal control system and method for chemical and biochemical reactions |
| CN106669876A (en) * | 2016-12-20 | 2017-05-17 | 芜湖市诺康生物科技有限公司 | Constant-temperature circulating water bath device |
| CN108465495A (en) * | 2018-03-22 | 2018-08-31 | 山西能源学院 | A kind of oil bath heating device of local cooling |
| CN109971617A (en) * | 2019-04-30 | 2019-07-05 | 郭嘉杰 | A kind of cryogenic process system of PCR amplification device |
| CN210656874U (en) * | 2019-05-28 | 2020-06-02 | 中元牧康(武汉)检测技术服务有限公司 | Fluorescent quantitative pcr instrument |
| CN211645193U (en) * | 2019-12-30 | 2020-10-09 | 郑州乐业生物科技有限公司 | PCR gene amplification instrument |
| CN213012873U (en) * | 2020-08-14 | 2021-04-20 | 北京岱美仪器有限公司 | Temperature control device for PCR instrument |
-
2021
- 2021-05-25 CN CN202110573132.3A patent/CN113201454A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19852733A1 (en) * | 1998-11-16 | 2000-05-25 | Max Planck Gesellschaft | Method and device for tempering a liquid bath |
| CN102046291A (en) * | 2008-04-04 | 2011-05-04 | It-Is国际有限公司 | Thermal control system and method for chemical and biochemical reactions |
| CN101565672A (en) * | 2008-04-21 | 2009-10-28 | 张永钢 | Liquid circulating gene amplification equipment |
| CN106669876A (en) * | 2016-12-20 | 2017-05-17 | 芜湖市诺康生物科技有限公司 | Constant-temperature circulating water bath device |
| CN108465495A (en) * | 2018-03-22 | 2018-08-31 | 山西能源学院 | A kind of oil bath heating device of local cooling |
| CN109971617A (en) * | 2019-04-30 | 2019-07-05 | 郭嘉杰 | A kind of cryogenic process system of PCR amplification device |
| CN210656874U (en) * | 2019-05-28 | 2020-06-02 | 中元牧康(武汉)检测技术服务有限公司 | Fluorescent quantitative pcr instrument |
| CN211645193U (en) * | 2019-12-30 | 2020-10-09 | 郑州乐业生物科技有限公司 | PCR gene amplification instrument |
| CN213012873U (en) * | 2020-08-14 | 2021-04-20 | 北京岱美仪器有限公司 | Temperature control device for PCR instrument |
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Application publication date: 20210803 |