CN113197098A - Inoculation method for shortening rapid propagation growth cycle of phalaenopsis - Google Patents
Inoculation method for shortening rapid propagation growth cycle of phalaenopsis Download PDFInfo
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- CN113197098A CN113197098A CN202110633453.8A CN202110633453A CN113197098A CN 113197098 A CN113197098 A CN 113197098A CN 202110633453 A CN202110633453 A CN 202110633453A CN 113197098 A CN113197098 A CN 113197098A
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- 238000011081 inoculation Methods 0.000 title claims abstract description 56
- 238000000034 method Methods 0.000 title claims abstract description 35
- 241001505935 Phalaenopsis Species 0.000 title claims abstract description 11
- 238000004904 shortening Methods 0.000 title claims abstract description 9
- 238000005520 cutting process Methods 0.000 claims abstract description 47
- 239000001963 growth medium Substances 0.000 claims abstract description 36
- 239000002609 medium Substances 0.000 claims description 17
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 16
- 238000005286 illumination Methods 0.000 claims description 12
- 240000008042 Zea mays Species 0.000 claims description 8
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 8
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 8
- 229960005070 ascorbic acid Drugs 0.000 claims description 8
- 235000010323 ascorbic acid Nutrition 0.000 claims description 8
- 239000011668 ascorbic acid Substances 0.000 claims description 8
- 235000005822 corn Nutrition 0.000 claims description 8
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 8
- 235000013575 mashed potatoes Nutrition 0.000 claims description 8
- 235000018290 Musa x paradisiaca Nutrition 0.000 claims description 6
- 238000012258 culturing Methods 0.000 claims description 5
- 239000006870 ms-medium Substances 0.000 claims description 3
- 240000008790 Musa x paradisiaca Species 0.000 claims 2
- 241000234295 Musa Species 0.000 description 6
- 230000000052 comparative effect Effects 0.000 description 6
- 238000003780 insertion Methods 0.000 description 6
- 230000037431 insertion Effects 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 230000035784 germination Effects 0.000 description 5
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 description 3
- 239000004062 cytokinin Substances 0.000 description 3
- 235000021015 bananas Nutrition 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 244000127818 Phalaenopsis amabilis Species 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses an inoculation method for shortening the rapid propagation and growth cycle of phalaenopsis, the bud body of phalaenopsis contains at least one of strong bud and weak bud, and the strong bud meets the following conditions: the leaf color is dark green, the stem and the leaf in the lateral bud are differentiated, and the weak bud meets the following conditions: the leaves are light green or white, and the stems and leaves in the lateral buds are not differentiated; the stem of the strong bud is thicker than the stem of the weak bud; the method includes at least one of strong bud cutting inoculation and weak bud cutting inoculation; the cutting and inoculation of the strong bud comprises the following steps: removing terminal buds of the strong buds, and inserting 1 strong bud as a unit into a first culture medium for culture; the weak bud cutting inoculation comprises the following steps: preserving the terminal buds of the weak buds, separating the weak buds along bud slits by straight knives, and inserting 2-3 weak buds as units into a second culture medium for culture; wherein the inserting depth of the strong bud and the weak bud in the culture medium is 0.15-0.25 of the height of the bud body; the method can shorten rapid propagation period.
Description
Technical Field
The invention relates to rapid propagation of phalaenopsis, in particular to an inoculation method for shortening the rapid propagation growth cycle of phalaenopsis.
Background
In the process of asexual propagation and rapid propagation of butterfly orchid, the aim of rapid propagation is to obtain enough buds as soon as possible, and the rapid propagation is realized by the division and the propagation of buds at present. The existing method for rapid propagation of buds generally comprises the following steps: removing terminal bud dominance, and culturing in 1-2 units with MS culture medium containing cell division number.
Although the existing rapid propagation method can obtain enough buds, the method has the following defects: 1. a large amount of cytokinin is required in the culture medium, and the high amount of cytokinin can cause variation risk in later growth of buds; 2. the culture period of the method is more than 45 days, the production cost is increased due to overlong period, and the increase of the productivity is also not facilitated.
Disclosure of Invention
The invention aims to provide an inoculation method for shortening the rapid propagation growth period of phalaenopsis, which can shorten the rapid propagation period and effectively reduce the risk of variation of buds in later growth.
In order to achieve the aim, the invention provides an inoculation method for shortening the rapid propagation growth cycle of phalaenopsis, and strong buds meet the following conditions: the leaf color is dark green, the stem and the leaf in the lateral bud are differentiated, and the weak bud meets the following conditions: the leaves are light green or white, and the stems and leaves in the lateral buds are not differentiated; the stem of the strong bud is thicker than the stem of the weak bud; the method includes at least one of strong bud cutting inoculation and weak bud cutting inoculation;
the cutting and inoculation of the strong bud comprises the following steps: removing terminal buds of the strong buds, and inserting 1 strong bud as a unit into a first culture medium for culture; the weak bud cutting inoculation comprises the following steps: preserving the terminal buds of the weak buds, separating the weak buds along bud slits by straight knives, and inserting 2-3 weak buds as units into a second culture medium for culture; wherein the inserting depth of the strong bud and the weak bud in the culture medium is 0.15-0.25 of the height of the bud body.
Preferably, the terminal bud removal of the strong bud comprises: cutting along the intersection of the topmost leaf and stem of the bud.
Preferably, in the cutting inoculation of the strong bud, the culture at least satisfies the following conditions: the illumination is 1500-.
Preferably, in the weak bud cutting inoculation, the culture at least meets the following conditions: the illumination is 1500-.
Preferably, the total time of culture in the strong bud cutting inoculation and the weak bud cutting inoculation is each independently 20 to 25 days.
Preferably, the first medium and the second medium each independently comprise: MS culture medium, mashed potato, mashed banana, ascorbic acid and corn juice.
Preferably, in the first culture medium and the second culture medium, the weight ratio of the MS culture medium, the mashed potato, the mashed banana, the ascorbic acid and the corn juice is 100: 8-12: 3-5: 0.1-0.3: 1-2.5.
In the technical scheme, the inventor carries out cutting inoculation on the strong bud and the weak bud in a distinguishing way, and aiming at the strong bud, the cutting inoculation comprises the following steps: removing terminal buds of the strong buds, and inserting 1 strong bud as a unit into a first culture medium for culture; for weak shoots, cutting inoculation included: the terminal buds of the weak buds are reserved, the weak buds are divided along bud slits and are inserted into a second culture medium for culture by taking 2-3 weak buds as units, and the survival rate of buds can be effectively ensured by taking 2-3 weak buds as units; after cutting and inoculation, enough robust buds can be obtained in a suitable production environment for about 3 weeks, so that the bud rapid propagation is completed. Compared with 45 days, the rapid propagation period is greatly improved, so that the cost is effectively reduced, and the capacity is expanded.
Additional features and advantages of the invention will be set forth in the detailed description which follows.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention. In the drawings:
FIG. 1 is a diagram showing the state of a bud after completion of cutting inoculation of a strong bud in example 1;
FIG. 2 is a diagram showing the state of the shoot body after the completion of the cutting inoculation of the weak shoot in example 2.
Detailed Description
The following describes in detail specific embodiments of the present invention. It should be understood that the detailed description and specific examples, while indicating the present invention, are given by way of illustration and explanation only, not limitation.
The invention provides an inoculation method for shortening the rapid propagation and growth period of phalaenopsis, and strong buds meet the following conditions: the leaf color is dark green, the stem and the leaf in the lateral bud are differentiated, and the weak bud meets the following conditions: the leaves are light green or white, and the stems and leaves in the lateral buds are not differentiated; the stem of the strong bud is thicker than the stem of the weak bud; the method includes at least one of strong bud cutting inoculation and weak bud cutting inoculation;
the cutting and inoculation of the strong bud comprises the following steps: removing terminal buds of the strong buds, and inserting 1 strong bud as a unit into a first culture medium for culture; the weak bud cutting inoculation comprises the following steps: preserving the terminal buds of the weak buds, separating the weak buds along bud slits by straight knives, and inserting 2-3 weak buds as units into a second culture medium for culture; the inserting depth of the strong bud and the weak bud in the culture medium is 0.15-0.25 of the height of the bud body, the inserting depth of the strong bud and the weak bud in the culture medium cannot be too deep, if the inserting depth is too deep, the bud is weak, the color of the bud body is light and white, and if the inserting depth is too light, the bud body can fall off from the culture medium.
In the above weak bud cutting inoculation, the height of the removed terminal bud is not particularly limited, but in order to further enhance the germination effect of the bud body, it is preferable that the removed terminal bud of the strong bud comprises: cutting along the intersection of the topmost leaf and stem of the bud.
In the above-mentioned cutting inoculation of a strong bud, the culture conditions in the cutting inoculation of a strong bud to be removed are not particularly limited, but in order to further improve the germination effect of a bud, it is preferable that in the cutting inoculation of a strong bud, the culture satisfies at least the following conditions: the illumination is 1500-.
In the above weak shoot cutting inoculation, the culture conditions in the weak shoot cutting inoculation to be removed are not particularly limited, but in order to further improve the germination effect of the sprouts, it is preferable that in the weak shoot cutting inoculation, the culture satisfies at least the following conditions: the illumination is 1500-.
In the above inoculation method, the total time of culture in the strong bud cutting inoculation and the weak bud cutting inoculation may be selected within a wide range, but in order to shorten the period and obtain a sufficient number of buds, it is preferable that the total time of culture in the strong bud cutting inoculation and the weak bud cutting inoculation is each independently 20 to 25 days.
In the above inoculation method, the components in the first medium and the second medium may be selected within a wide range, but for a sufficient number of sprouts, it is preferable that the first medium and the second medium each independently contain: MS culture medium, mashed potato, mashed banana, ascorbic acid and corn juice.
In the above embodiment, the contents of the components in the first and second culture media may be selected within a wide range, but for sufficient sprouts, it is preferable that the weight ratio of the MS culture medium, mashed potato, mashed banana, ascorbic acid and corn juice is 100: 8-12: 3-5: 0.1-0.3: 1-2.5.
As can be seen from the above, the first culture medium and the second culture medium do not contain cytokinin, so that the risk of later-stage variation of buds can be effectively reduced.
The present invention will be described in detail below by way of examples.
Example 1
Cutting, inoculating and culturing the strong bud, wherein the strong bud meets the following conditions: the leaves are dark green, the stems and leaves in the lateral buds are differentiated, and the stems are thick;
cutting along the intersection of the topmost leaf and stem of the strong bud, removing the terminal bud of the strong bud, inserting 1 strong bud as a unit into a culture medium, and culturing for 21 days, wherein the insertion depth of the strong bud in the culture medium is 0.20 of the height of a bud body. The culture conditions were: the illumination is 1800lux, the average temperature is 25 ℃, the illumination is 12 hours per day, and the illumination time is 7-19 points; the components and contents of the culture medium are as follows: the weight ratio of the components is 100: 10: 4: 0.2: 1.5 MS medium, mashed potatoes, mashed bananas, ascorbic acid and corn juice.
The culture results are shown in FIG. 1.
Example 2
Cutting weak buds, inoculating and culturing the weak buds, wherein the weak buds meet the following conditions: the leaves are light green or white, and the stems and leaves in the lateral buds are not differentiated;
the terminal buds of the weak buds are reserved, the weak buds are divided along the bud slit, the weak buds are inserted into a culture medium for 21 days by taking 2 weak buds as a unit, and the insertion depth of the weak buds in the culture medium is 0.20 of the height of a bud body. The culture conditions were: the illumination is 1800lux, the temperature is 25 ℃, the illumination is 12 hours per day, and the illumination time is 7-19 points; the components and contents of the culture medium are as follows: the weight ratio of the components is 100: 10: 4: 0.2: 1.5 MS medium, mashed potatoes, mashed bananas, ascorbic acid and corn juice.
The culture results are shown in FIG. 2.
Example 3
The procedure of example 1 was followed, except that the depth of the strong bud inserted into the medium was 0.15 of the height of the bud.
Example 4
The procedure of example 1 was followed, except that the depth of the strong bud inserted into the medium was 0.25 of the height of the bud.
Example 5
The procedure of example 2 was followed, except that 3 of the weak shoots were inserted into the medium in units of 0.15 of the height of the bud.
Example 6
The procedure of example 2 was followed, except that 3 of the weak shoots were inserted into the medium in units of 0.25 of the height of the bud.
Comparative example 1
The procedure of example 1 was followed, except that the depth of the strong bud inserted into the medium was 0.10 of the height of the bud.
Comparative example 2
The procedure of example 1 was followed, except that the depth of the strong bud inserted into the medium was 0.30 of the height of the bud.
Comparative example 3
The procedure of example 2 was followed, except that the insertion depth of the weak shoots in the medium was each 0.15 of the height of the bud.
Comparative example 4
The procedure of example 2 was followed, except that the insertion depth of the weak shoots in the medium was each 0.30 of the height of the bud.
Comparative example 5
The procedure of example 2 was followed, except that 1 of the weak shoots was inserted into the medium for cultivation.
Example of detection
After the culture of the above examples is finished, the germination of the buds is counted, and the details are shown in Table 1.
TABLE 1
As can be seen from the above table and fig. 1-2, the present invention can achieve rapid propagation of buds (culture period of the prior art is more than 45 days) in about 3 weeks, thereby greatly shortening the rapid propagation period, wherein, as can be seen from comparative examples 1-5, the depth of bud insertion into the culture medium, the height of top bud removal and the root tree in the weak bud insertion unit all have significant effects on the germination effect of the buds.
The preferred embodiments of the present invention have been described in detail, however, the present invention is not limited to the specific details of the above embodiments, and various simple modifications may be made to the technical solution of the present invention within the technical idea of the present invention, and these simple modifications are within the protective scope of the present invention.
It should be noted that the various technical features described in the above embodiments can be combined in any suitable manner without contradiction, and the invention is not described in any way for the possible combinations in order to avoid unnecessary repetition.
In addition, any combination of the various embodiments of the present invention is also possible, and the same should be considered as the disclosure of the present invention as long as it does not depart from the spirit of the present invention.
Claims (7)
1. An inoculation method for shortening the rapid propagation growth cycle of phalaenopsis, which is characterized in that the bud body of phalaenopsis contains at least one of strong bud and weak bud, wherein the strong bud meets the following conditions: the leaf color is dark green, the stem and the leaf in the lateral bud are differentiated, and the weak bud meets the following conditions: the leaves are light green or white, and the stems and leaves in the lateral buds are not differentiated; the stem of the strong bud is thicker than the stem of the weak bud; the method includes at least one of strong bud cutting inoculation and weak bud cutting inoculation;
the cutting and inoculation of the strong bud comprises the following steps: removing terminal buds of the strong buds, and inserting 1 strong bud as a unit into a first culture medium for culture; the weak bud cutting inoculation comprises the following steps: preserving the terminal buds of the weak buds, separating the weak buds along bud slits by straight knives, and inserting 2-3 weak buds as units into a second culture medium for culture; wherein the inserting depth of the strong bud and the weak bud in the culture medium is 0.15-0.25 of the height of the bud body.
2. The inoculation method of claim 1, wherein the removal of terminal buds of the strong buds comprises: cutting along the intersection of the topmost leaf and stem of the bud.
3. The inoculation method according to claim 1, wherein in the cutting inoculation of the strong bud, culturing satisfies at least the following conditions: the illumination is 1500-.
4. The inoculation method according to claim 1, wherein in the weak shoot cutting inoculation, at least the following conditions are satisfied: the illumination is 1500-.
5. The inoculation method according to claim 1, wherein the total time of culture in the strong bud-cutting inoculation and the weak bud-cutting inoculation is each independently 20 to 25 days.
6. The method of inoculating as claimed in claim 1, wherein the first and second culture media each independently comprise: MS culture medium, mashed potato, mashed banana, ascorbic acid and corn juice.
7. The inoculation method according to claim 1, wherein in the first medium, the second medium, the weight ratio of MS medium, mashed potato, mashed banana, ascorbic acid and corn juice is 100: 8-12: 3-5: 0.1-0.3: 1-2.5.
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| CN202110633453.8A CN113197098A (en) | 2021-06-07 | 2021-06-07 | Inoculation method for shortening rapid propagation growth cycle of phalaenopsis |
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| CN202110633453.8A CN113197098A (en) | 2021-06-07 | 2021-06-07 | Inoculation method for shortening rapid propagation growth cycle of phalaenopsis |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005168399A (en) * | 2003-12-11 | 2005-06-30 | Sapporo Breweries Ltd | Method for producing phalaenopsis clone seedling |
| CN104604687A (en) * | 2015-01-29 | 2015-05-13 | 赤峰市农牧科学研究院 | Method for inducing clustered shoots to rapidly propagate butterfly orchid by utilizing pedicle stem segments after bud cutting |
| CN105875414A (en) * | 2016-06-13 | 2016-08-24 | 陈春满 | Method for cultivating butterfly orchid by promoting rapid proliferation of butterfly orchid protocorm-like body |
| CN108243944A (en) * | 2018-01-24 | 2018-07-06 | 青州市亚泰农业科技有限公司 | A kind of iris rapid breeding method and fine quality tissue culture propagation |
-
2021
- 2021-06-07 CN CN202110633453.8A patent/CN113197098A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005168399A (en) * | 2003-12-11 | 2005-06-30 | Sapporo Breweries Ltd | Method for producing phalaenopsis clone seedling |
| CN104604687A (en) * | 2015-01-29 | 2015-05-13 | 赤峰市农牧科学研究院 | Method for inducing clustered shoots to rapidly propagate butterfly orchid by utilizing pedicle stem segments after bud cutting |
| CN105875414A (en) * | 2016-06-13 | 2016-08-24 | 陈春满 | Method for cultivating butterfly orchid by promoting rapid proliferation of butterfly orchid protocorm-like body |
| CN108243944A (en) * | 2018-01-24 | 2018-07-06 | 青州市亚泰农业科技有限公司 | A kind of iris rapid breeding method and fine quality tissue culture propagation |
Non-Patent Citations (2)
| Title |
|---|
| 彭娇等: "基于再生不定芽的蝴蝶兰继代扩繁体系建立", 《中国农学通报》 * |
| 王常芸等: "蝴蝶兰组培快繁技术体系研究", 《北方园艺》 * |
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