CN113189316A - Method for detecting water isotope tracer in exhaled air and exhaled air condensate - Google Patents
Method for detecting water isotope tracer in exhaled air and exhaled air condensate Download PDFInfo
- Publication number
- CN113189316A CN113189316A CN202110400280.5A CN202110400280A CN113189316A CN 113189316 A CN113189316 A CN 113189316A CN 202110400280 A CN202110400280 A CN 202110400280A CN 113189316 A CN113189316 A CN 113189316A
- Authority
- CN
- China
- Prior art keywords
- sample
- water
- exhaled breath
- background
- condensate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 102
- 238000000034 method Methods 0.000 title claims abstract description 31
- 239000000700 radioactive tracer Substances 0.000 title claims abstract description 22
- 210000001035 gastrointestinal tract Anatomy 0.000 claims abstract description 17
- 241000894006 Bacteria Species 0.000 claims abstract description 12
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 42
- 239000002253 acid Substances 0.000 claims description 17
- 238000004458 analytical method Methods 0.000 claims description 16
- 238000012360 testing method Methods 0.000 claims description 15
- 238000005194 fractionation Methods 0.000 claims description 13
- 239000008213 purified water Substances 0.000 claims description 12
- 239000001257 hydrogen Substances 0.000 claims description 11
- 229910052739 hydrogen Inorganic materials 0.000 claims description 11
- 150000001720 carbohydrates Chemical class 0.000 claims description 7
- 239000003651 drinking water Substances 0.000 claims description 6
- 235000020188 drinking water Nutrition 0.000 claims description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 5
- 239000008103 glucose Substances 0.000 claims description 5
- QVGXLLKOCUKJST-OUBTZVSYSA-N oxygen-17 atom Chemical class [17O] QVGXLLKOCUKJST-OUBTZVSYSA-N 0.000 claims description 5
- QVGXLLKOCUKJST-NJFSPNSNSA-N oxygen-18 atom Chemical class [18O] QVGXLLKOCUKJST-NJFSPNSNSA-N 0.000 claims description 5
- 208000015181 infectious disease Diseases 0.000 claims description 4
- 230000035622 drinking Effects 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 3
- 230000000155 isotopic effect Effects 0.000 claims description 3
- 229930091371 Fructose Natural products 0.000 claims description 2
- 239000005715 Fructose Substances 0.000 claims description 2
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 claims description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 2
- 229930006000 Sucrose Natural products 0.000 claims description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 2
- 239000005720 sucrose Substances 0.000 claims description 2
- 125000004435 hydrogen atom Chemical class [H]* 0.000 claims 3
- 235000007983 food acid Nutrition 0.000 claims 1
- 235000000346 sugar Nutrition 0.000 claims 1
- 150000008163 sugars Chemical class 0.000 claims 1
- 238000000926 separation method Methods 0.000 abstract description 5
- 239000007789 gas Substances 0.000 description 17
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 15
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 12
- 239000001301 oxygen Substances 0.000 description 12
- 229910052760 oxygen Inorganic materials 0.000 description 12
- 241000590002 Helicobacter pylori Species 0.000 description 10
- 229940037467 helicobacter pylori Drugs 0.000 description 10
- 238000001514 detection method Methods 0.000 description 9
- 210000002784 stomach Anatomy 0.000 description 9
- 239000004202 carbamide Substances 0.000 description 8
- 150000002431 hydrogen Chemical class 0.000 description 8
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 6
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 208000035143 Bacterial infection Diseases 0.000 description 4
- 108010020056 Hydrogenase Proteins 0.000 description 4
- 208000022362 bacterial infectious disease Diseases 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 238000003745 diagnosis Methods 0.000 description 4
- 108010046334 Urease Proteins 0.000 description 3
- 229910002092 carbon dioxide Inorganic materials 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- RGHNJXZEOKUKBD-SQOUGZDYSA-N D-gluconic acid Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C(O)=O RGHNJXZEOKUKBD-SQOUGZDYSA-N 0.000 description 2
- 206010019375 Helicobacter infections Diseases 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000002775 capsule Substances 0.000 description 2
- 239000001569 carbon dioxide Substances 0.000 description 2
- 210000001156 gastric mucosa Anatomy 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 230000029058 respiratory gaseous exchange Effects 0.000 description 2
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 description 1
- RGHNJXZEOKUKBD-UHFFFAOYSA-N D-gluconic acid Natural products OCC(O)C(O)C(O)C(O)C(O)=O RGHNJXZEOKUKBD-UHFFFAOYSA-N 0.000 description 1
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 description 1
- 241000237858 Gastropoda Species 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 230000037358 bacterial metabolism Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 230000009982 effect on human Effects 0.000 description 1
- 230000037149 energy metabolism Effects 0.000 description 1
- 230000002550 fecal effect Effects 0.000 description 1
- 238000002575 gastroscopy Methods 0.000 description 1
- 239000000174 gluconic acid Substances 0.000 description 1
- 235000012208 gluconic acid Nutrition 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 230000010226 intestinal metabolism Effects 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 239000001630 malic acid Substances 0.000 description 1
- 235000011090 malic acid Nutrition 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000011975 tartaric acid Substances 0.000 description 1
- 235000002906 tartaric acid Nutrition 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/42—Detecting, measuring or recording for evaluating the gastrointestinal, the endocrine or the exocrine systems
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Pathology (AREA)
- Animal Behavior & Ethology (AREA)
- Hematology (AREA)
- Medical Informatics (AREA)
- Biophysics (AREA)
- Surgery (AREA)
- Physiology (AREA)
- Gastroenterology & Hepatology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Heart & Thoracic Surgery (AREA)
- Urology & Nephrology (AREA)
- Endocrinology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
The invention relates to a method for detecting a tracer of water isotopes in exhaled breath and exhaled breath condensate, which is characterized in that the water isotopes are used as the tracer to detect the difference of the separation ratios of the water isotopes in the exhaled breath so as to judge whether bacteria exist in the gastrointestinal tract.
Description
Technical Field
The invention belongs to the technical field of exhaled breath detection, and particularly relates to a detection method of a water isotope tracer in exhaled breath and exhaled breath condensate.
Background
Water is the most abundant component in the human body. Water plays an extremely important role in chemical reactions that occur in various metabolisms of the human body. The exhaled breath contains a large amount of water vapor, and the fractionation of water in the digestive tract is related to the respiration process and reflects the respiration processIn the isotopic character of exhaled water vapor. Testing deuterated hydrogen in exhaled breath water vapor or water vapor condensate by high-sensitivity water isotope analyzer
Oxygen 17
Oxygen 18
The isotope fractionation ratio of (1) and the isotope fractionation in water is influenced by a digestive tract liquid having impaired or abnormal water absorption, and thus can be used as a tracer for the occurrence of various digestive tract deformations.
The human intestinal tract is populated with a large number of bacteria which have a very important effect on human health. Hydrogen is a by-product of human intestinal bacterial metabolism, and most of the hydrogen is consumed by other bacteria oxidation, some of which are absorbed or excreted with the fart. Hydrogenases on the other hand are metalloenzymes present in anaerobic microorganisms of nature, which catalyze reversible chemical reactions, either the oxidation of hydrogen or the reduction of protons. Hydrogenase is also present in human body, for example, in the infected person of helicobacter pylori in human gastrointestinal tract, and the hydrogenase can take hydrogen produced by human intestinal metabolism as substrate for energy metabolism, so that the hydrogen participates in human metabolic process.
The hydrogen in the gastrointestinal tract of human body participates in the reaction in the process of catalyzing and metabolizing by hydrogenase enzyme, so that the isotope fractionation ratio in the water is caused to generate an exchange reaction, and the deuterated hydrogen in the water in the exhaled gas steam or steam condensate is tested by a high-sensitivity water isotope analyzer
Oxygen 17
Oxygen 18
The isotope fractionation ratio of (a) can be used to aid in the diagnosis of the risk of bacterial infection in certain humans. Provides reference for the clinical auxiliary diagnosis of the exhaled breath and the exhaled breath condensate water isotope used as a tracer.
Problems in the prior art
Taking the detection of helicobacter pylori infection in the stomach as an example:
invasive examination method
It is used for examining helicobacter pylori by clamping 1-2 pieces of gastric mucosa tissues under a gastroscope at the same time of the gastroscope examination. The method comprises the following steps: fast urease test, gastric mucosa tissue section staining microscopy and helicobacter pylori culture. As helicobacter pylori in the stomach is distributed in a 'focus', the invasive examination method can only reflect the existence of helicobacter pylori at the material-taking part and hardly reflects the general picture of the helicobacter pylori infection of the stomach.
Non-invasive examination method
It is a method which does not depend on gastroscopy, and the patient has no pain, but the condition of the stomach illness cannot be clarified. The method can reflect the infection condition of helicobacter pylori in the whole stomach, and overcome the false negative caused by the 'focal' distribution of bacteria.
The method mainly comprises the following steps: urea breath test, fecal helicobacter pylori antigen detection, and serum helicobacter pylori antibody detection.
The urea breath test comprises: c-13Breath test of urea, C-14Urea breath test.
If helicobacter pylori is present in the stomach, the bacterium secretes urease into the stomach. Oral administration of C-13Or C-14Marked urea capsule, after the urea capsule enters the stomach, the urease in the stomach can decompose the urea into ammonia and C-13Or C-14Labelled CO2(carbon dioxide), carbon dioxide formed by hydrolysis of urea, is absorbed into the blood, enters the lungs with the blood stream and is emitted as a gas. Detecting the presence or absence of labeled C-13Or C-14It is possible to determine whether the patient is infected with helicobacter pylori and to detect C-13Or C-14The number of the pyloric snail is preliminarily judgedThe number of bacilli.
Disclosure of Invention
The invention aims to provide a method for detecting a water isotope tracer in exhaled breath and exhaled breath condensate, which can detect the difference of the separation ratio of water isotopes in exhaled breath by taking the water isotopes as the tracer so as to assist in judging whether the gastrointestinal tract is infected by bacteria.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for detecting a water isotope tracer in exhaled breath and exhaled breath condensate, said method comprising the steps of:
1) selecting drinking water as a background water sample A;
2) collecting an expired air background sample B-1 or an expired air condensate background sample B-2 of a subject in a fasting state;
3) the subject in the step 2) drinks the drinking water sample in the step 1), and the expired air actual sample C-1 or the expired air condensate actual sample C-2 of the subject is collected again after a certain preset time (30-60 min);
4) and (3) carrying out water isotope analysis on the exhaled breath background sample B-1 or the exhaled breath condensate background sample B-2 collected in the step 2) and the exhaled breath actual sample C-1 or the exhaled breath condensate actual sample C-2 collected in the step 3), respectively analyzing isotope fractionation ratios of deuterated hydrogen, oxygen 17 and oxygen 18 of water, and establishing a database for subsequent comparison.
By analyzing the deuterated hydrogen of the exhaled breath of the subject or water in a sample of the exhaled breath before and after drinking
Oxygen 17
Oxygen 18
To assist in determining whether a subject is at risk of bacterial infection of the gastrointestinal tract, and thereby of the bacteriumThe risk provides an auxiliary judgment method, and also can provide reference for the exhaled breath and the exhaled breath condensate water isotope used as a tracer for clinical auxiliary diagnosis.
And meanwhile, a database is established for comparing different subsequent subjects after receiving the detection of the water isotope tracer in the exhaled breath and the exhaled breath condensate, so that a suggestion whether the subsequent subjects need to go to a hospital for detailed examination is given.
The isotope fractionation ratio detection used in the present invention may be a commercially available high-precision water isotope analyzer such as a Picaro isotope water analyzer, or an LGR water isotope analyzer.
As a preferable scheme of the present invention, in step 1), the background water sample a includes purified water or a purified water solution in which an edible acid is dissolved.
The pure water in the technical scheme can be commercial pure water, and when the pure water is taken as a background water sample, a water isotope analysis test is carried out on the background water sample; when the acid solution with pure water as a solvent is used as a background water sample, the water isotope analysis test on the background water sample is not needed.
In a preferred embodiment of the present invention, the amount of the edible acid added to the purified water solution of the edible acid is 45-55mL of purified water per 1g of the edible acid.
For example, 1g of acid is dissolved in 45-55mL of pure water, or 2g of acid is dissolved in 90-110mL, or 3g of acid is dissolved in 135-165mL, or 4g of acid is dissolved in 180-220mL of pure water.
In a preferred embodiment of the present invention, the edible acid comprises citric acid or citric acid containing saccharide.
The edible acid in the present invention is not limited to citric acid, and may be an edible acid capable of dissolving saccharides, such as malic acid, gluconic acid, lactic acid, and tartaric acid.
In a preferred embodiment of the present invention, the citric acid containing saccharide comprises one or more of glucose, fructose and sucrose, and the mass ratio of saccharide to citric acid is 1:1-1: 5.
As a preferred scheme of the invention, when the background water sample A is pure water, the isotope analysis test is carried out on the background water sample A.
As a preferred embodiment of the present invention, the isotope analysis test is: and injecting the background water sample A into a sealed heating container, and extracting a water vapor sample for analysis to serve as background water vapor.
In a preferred embodiment of the present invention, in the step 4), the isotopic fractionation ratios of deuterated hydrogen, oxygen 17 and oxygen 18 of the background water sample a, the background exhaled air sample B-1 in the fasting state and the actual exhaled air sample C-1 after drinking for 30-60min are compared to assist in determining whether the subject is at risk of gastrointestinal tract infection by bacteria; or comparing the deuterated hydrogen of the background water sample A, the expired gas background sample B-2 in the fasting state and the actual expired gas condensate sample C-2 after drinking
Oxygen 17
Oxygen 18
To assist in determining whether a subject is at risk for bacterial infection of the gastrointestinal tract.
In a preferred embodiment of the present invention, in the step 2), the volume of the collected exhaled air sample in the steps 3) and 4) is 200-500mL, and the volume of the collected exhaled air condensate sample is 0.5-1 mL; in the step 3), the preset certain time is 30-60 min.
As a preferable scheme of the invention, in the step 4), the amount of the sample for sample injection analysis is 1-5 μ L.
Compared with the prior art, the invention has the following beneficial effects:
1) the invention detects the difference of the separation ratio of the water isotopes in the exhaled breath by using the water isotopes as the tracer, thereby assisting in judging whether the gastrointestinal tract of the subject is infected by bacteria;
2) the invention can provide an auxiliary judgment method for the infection risk of mesobacteria, and also can provide reference for the use of the exhaled breath and the exhaled breath condensate water isotope as a tracer for clinical auxiliary diagnosis.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
The embodiment provides a detection method of a water isotope tracer in exhaled breath and exhaled breath condensate, which comprises the following steps:
1) selecting purified water as a background water sample A; injecting a background water sample A into a sealed heating container, and extracting a water vapor sample to analyze the water vapor sample to be used as background water vapor;
2) collecting an expired air background sample B-1 or an expired air condensate background sample B-2 of a subject in a fasting state; the volume of the collected exhaled gas sample is 200-500mL, and the volume of the collected exhaled gas condensate sample is 0.5-1 mL;
3) the subject in the step 2) drinks the drinking water sample in the step 1), and the actual exhaled breath sample C-1 or the actual exhaled breath condensate sample C-2 of the subject is collected again after 30-60 min; the volume of the collected exhaled gas sample is 200-500mL, and the volume of the collected exhaled gas condensate sample is 0.5-1 mL;
4) carrying out water isotope analysis on the exhaled breath background sample B-1 or the exhaled breath condensate background sample B-2 collected in the step 2) and the exhaled breath actual sample C-1 or the exhaled breath condensate actual sample C-2 collected in the step 3), and respectively analyzing the deuterated hydrogen of the water
Oxygen 17
Oxygen 18
Isotope fractionation ratio of (a); wherein, the sample dosage for sample injection analysis is 1-5 muL.
Example 2
The embodiment provides a detection method of a water isotope tracer in exhaled breath and exhaled breath condensate, which comprises the following steps:
1) selecting purified water in which citric acid is dissolved as a background water sample A, wherein the dosage of the selected purified water is 200mL, and the dosage of the selected citric acid is 4 g;
2) collecting an expired air background sample B-1 or an expired air condensate background sample B-2 of a subject in a fasting state; the volume of the collected exhaled gas sample is 200-500mL, and the volume of the collected exhaled gas condensate sample is 0.5-1 mL;
3) the subject in the step 2) drinks the drinking water sample in the step 1), and the actual exhaled breath sample C-1 or the actual exhaled breath condensate sample C-2 of the subject is collected again after 30 min; the volume of the collected exhaled gas sample is 200-500mL, and the volume of the collected exhaled gas condensate sample is 0.5-1 mL;
4) the expired gas background sample B-1 or the expired gas condensate background sample B-2 collected in the step 2) and the expired gas condensate background sample B-2 collected in the step 3)
The collected expired gas sample C-1 or the expired gas condensate actual sample C-2 is subjected to water isotope analysis to respectively analyze the deuterated hydrogen of water
Oxygen 17
Oxygen 18
Isotope fractionation ratio of (a); wherein, the sample dosage for sample injection analysis is 1-5 muL.
Example 3
The method is the same as example 2, and the only difference is that citric acid containing glucose is dissolved in purified water as a background water sample A, wherein the mass ratio of the glucose to the citric acid is 1:5, the dosage of the selected purified water is 200mL, and the dosage of the selected citric acid containing glucose is 4 g.
Table 1 shows the data of the tests of different subjects, wherein the subjects were tested by the method of the present invention and then subjected to C13Breath tests and comparisons were made to verify that the method of the invention can be used to assist in determining whether there is a risk of bacterial infection of the gastrointestinal tract.
TABLE 1 test data
Therefore, the invention detects the difference of the separation ratio of the water isotopes in the exhaled breath by using the water isotopes as the tracer, thereby assisting in judging whether the gastrointestinal tract of the subject is infected by the bacteria or not, and enabling the subject to go to a hospital for further examination in the later period to diagnose that the gastrointestinal tract is infected by the bacteria; the invention has higher accuracy and better assistance and inspiration.
The water isotopes of different subjects are used as tracer, data for detecting the difference of the separation ratios of the water isotopes in the exhaled breath are collected, a curve is conveniently generated to form a database, comparison is carried out after subsequent subjects are detected, and the suggestion that whether the subjects need to go to a hospital for detailed examination can be judged from the curve.
While the invention has been described with respect to a preferred embodiment, it will be understood by those skilled in the art that the foregoing and other changes, omissions and deviations in the form and detail thereof may be made without departing from the scope of this invention. Those skilled in the art can make various changes, modifications and equivalent arrangements, which are equivalent to the embodiments of the present invention, without departing from the spirit and scope of the present invention, and which may be made by utilizing the techniques disclosed above; meanwhile, any changes, modifications and variations of the above-described embodiments, which are equivalent to those of the technical spirit of the present invention, are within the scope of the technical solution of the present invention.
Claims (10)
1. A method for detecting a water isotope tracer in exhaled breath and exhaled breath condensate, comprising the steps of:
1) selecting drinking water as a background water sample A;
2) collecting an expired air background sample B-1 or an expired air condensate background sample B-2 of a subject in a fasting state;
3) the subject in the step 2) drinks the drinking water sample in the step 1), and the actual exhaled breath sample C-1 or the actual exhaled breath condensate sample C-2 of the subject is collected again after a certain preset time;
4) and (3) carrying out water isotope analysis on the exhaled breath background sample B-1 or the exhaled breath condensate background sample B-2 collected in the step 2) and the exhaled breath actual sample C-1 or the exhaled breath condensate actual sample C-2 collected in the step 3), respectively analyzing isotope fractionation ratios of deuterated hydrogen, oxygen 17 and oxygen 18 of water, and establishing a database for subsequent comparison.
2. The method according to claim 1, wherein the background water sample A comprises purified water or a purified water solution of dissolved edible acid in step 1).
3. The method of claim 2, wherein the amount of the edible acid added to the purified solution of the edible acid is 45-55mL per 1g of the acid in the purified water solution of the edible acid.
4. A method as claimed in claim 2 or 3, wherein the food acid comprises citric acid or citric acid containing sugars.
5. The method of claim 4, wherein the citric acid comprises a saccharide, the mass ratio of the saccharide to the citric acid is 1:1-1:5, and the saccharide comprises one or more of glucose, fructose or sucrose.
6. The method of claim 1, wherein the isotope analysis test is performed on the background water sample A when the background water sample A is pure water.
7. The method of claim 6, wherein the isotopic analysis test comprises: and injecting the background water sample A into a sealed heating container, and extracting a water vapor sample for analysis to serve as background water vapor.
8. The method for detecting the water isotope tracer in the exhaled breath and the exhaled breath condensate according to claim 1, wherein in the step 4), the isotope fractionation ratios of deuterated hydrogen, oxygen 17 and oxygen 18 in the background water sample A, the exhaled breath background sample B-1 in the fasting state and the actual exhaled breath sample C-1 after drinking are compared to assist in judging whether the subject is at risk of gastrointestinal tract infection by bacteria; or comparing the isotope fractionation ratios of deuterated hydrogen, oxygen 17 and oxygen 18 of the background water sample A, the background sample B-2 of the exhaled breath condensate in the fasting state and the actual sample C-2 of the exhaled breath condensate after drinking to assist in judging whether the gastrointestinal tract of the subject is infected by bacteria.
9. The method for detecting the water isotope tracer in the exhaled breath and the exhaled breath condensate according to claim 1, wherein the volume of the collected exhaled breath sample in the steps 2), 3) and 4) is 200-500mL, and the volume of the collected exhaled breath condensate sample is 0.5-1 mL; in the step 3), the preset certain time is 30-60 min.
10. The method for detecting the water isotope tracer in the exhaled breath and the exhaled breath condensate according to claim 1, wherein the amount of the sample used for sample analysis in step 4) is 1 to 5 μ L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110400280.5A CN113189316A (en) | 2021-04-14 | 2021-04-14 | Method for detecting water isotope tracer in exhaled air and exhaled air condensate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110400280.5A CN113189316A (en) | 2021-04-14 | 2021-04-14 | Method for detecting water isotope tracer in exhaled air and exhaled air condensate |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113189316A true CN113189316A (en) | 2021-07-30 |
Family
ID=76974060
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110400280.5A Pending CN113189316A (en) | 2021-04-14 | 2021-04-14 | Method for detecting water isotope tracer in exhaled air and exhaled air condensate |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113189316A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104822841A (en) * | 2012-10-19 | 2015-08-05 | 艾维萨制药公司 | Methods for detecting bacterial infections |
| CN105954212A (en) * | 2016-04-21 | 2016-09-21 | 长沙三相医疗器械有限公司 | System for diagnosing helicobacter pylori infection of human body based on spectrophotometer and method for detecting content of ammonia gas in expired gas of person |
-
2021
- 2021-04-14 CN CN202110400280.5A patent/CN113189316A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104822841A (en) * | 2012-10-19 | 2015-08-05 | 艾维萨制药公司 | Methods for detecting bacterial infections |
| CN105954212A (en) * | 2016-04-21 | 2016-09-21 | 长沙三相医疗器械有限公司 | System for diagnosing helicobacter pylori infection of human body based on spectrophotometer and method for detecting content of ammonia gas in expired gas of person |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Arola | Diagnosis of hypolactasia and lactose malabsorption | |
| CN107036852B (en) | Expiration testing device | |
| Bond Jr et al. | Factors influencing pulmonary methane excretion in man: an indirect method of studying the in situ metabolism of the methane-producing colonic bacteria | |
| Perman et al. | Fasting breath hydrogen concentration: normal values and clinical application | |
| CN1101546C (en) | Method and kit for detecting helicobacter pylori | |
| US6902719B2 (en) | Reverse isotope dilution assay and lactose intolerance assay | |
| Caspary | Breath tests | |
| King et al. | Breath tests in the diagnosis of small intestine bacterial overgrowth | |
| JP2007192831A (en) | 13c glucose respiration test for diagnosing diabetes symptoms, and for monitoring blood sugar control | |
| Scaldaferri et al. | Intestinal gas production and gastrointestinal symptoms: from pathogenesis to clinical implication. | |
| WO2007054940A2 (en) | Breath test device and method | |
| D'Angelo et al. | Tricks for interpreting and making a good report on hydrogen and 13C breath tests | |
| Pouteau et al. | Production rate of acetate during colonic fermentation of lactulose: a stable-isotope study in humans | |
| JP4988857B2 (en) | Breath test method for detecting pathogenic microorganisms | |
| Pal et al. | Exploring triple-isotopic signatures of water in human exhaled breath, gastric fluid, and drinking water using integrated cavity output spectroscopy | |
| Barr et al. | Breath tests in pediatric gastrointestinal disorders: new diagnostic opportunities | |
| EP1427325A2 (en) | Management of gastro-intestinal disorders | |
| CN113189316A (en) | Method for detecting water isotope tracer in exhaled air and exhaled air condensate | |
| EP0253927A1 (en) | A breath test for measuring urease activity in the stomach using carbon isotope urea | |
| Anania et al. | Breath tests in pediatrics | |
| Berg et al. | Hydrogen concentration in expired air analyzed with a new hydrogen sensor, plasma glucose rise, and symptoms of lactose intolerance after oral administration of 100 gram lactose | |
| Nichols et al. | Overview of breath testing in clinical practice in North America | |
| COZZETTO | Radiocarbon Estimates Of Intestinal Absorption: Studies of Breath Excretion of C14O2 After Ingestion of Labeled Fatty Acids, Glucose, and Lactose | |
| Montes et al. | Breath hydrogen testing as a physiology laboratory exercise for medical students. | |
| Turner | Voc analysis by sift-ms, gc-ms, and electronic nose for diagnosing and monitoring disease |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210730 |
|
| RJ01 | Rejection of invention patent application after publication |