CN113186111A - Deep sea actinomycete rhodococcus erythropolis and application thereof in inhibiting aflatoxin - Google Patents
Deep sea actinomycete rhodococcus erythropolis and application thereof in inhibiting aflatoxin Download PDFInfo
- Publication number
- CN113186111A CN113186111A CN202011473063.0A CN202011473063A CN113186111A CN 113186111 A CN113186111 A CN 113186111A CN 202011473063 A CN202011473063 A CN 202011473063A CN 113186111 A CN113186111 A CN 113186111A
- Authority
- CN
- China
- Prior art keywords
- aflatoxin
- m2af
- rhodococcus erythropolis
- strain
- deep sea
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000187561 Rhodococcus erythropolis Species 0.000 title claims abstract description 22
- 241001446247 uncultured actinomycete Species 0.000 title claims abstract description 9
- 229930195730 Aflatoxin Natural products 0.000 title abstract description 24
- XWIYFDMXXLINPU-UHFFFAOYSA-N Aflatoxin G Chemical compound O=C1OCCC2=C1C(=O)OC1=C2C(OC)=CC2=C1C1C=COC1O2 XWIYFDMXXLINPU-UHFFFAOYSA-N 0.000 title abstract description 24
- 239000005409 aflatoxin Substances 0.000 title abstract description 24
- 230000002401 inhibitory effect Effects 0.000 title abstract description 10
- -1 pplication Species 0.000 title description 3
- 239000013049 sediment Substances 0.000 claims abstract description 5
- 238000004321 preservation Methods 0.000 claims abstract 2
- 244000005700 microbiome Species 0.000 claims description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 2
- 238000000855 fermentation Methods 0.000 abstract description 15
- 230000004151 fermentation Effects 0.000 abstract description 15
- 239000001963 growth medium Substances 0.000 abstract description 11
- 239000006228 supernatant Substances 0.000 abstract description 10
- 230000015572 biosynthetic process Effects 0.000 abstract description 9
- 238000003786 synthesis reaction Methods 0.000 abstract description 9
- 235000013305 food Nutrition 0.000 abstract description 5
- 239000013505 freshwater Substances 0.000 abstract description 4
- 238000003860 storage Methods 0.000 abstract description 4
- 230000000813 microbial effect Effects 0.000 abstract description 3
- 235000013361 beverage Nutrition 0.000 abstract description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 239000000047 product Substances 0.000 abstract 1
- 239000013543 active substance Substances 0.000 description 5
- 241000228230 Aspergillus parasiticus Species 0.000 description 4
- 238000012258 culturing Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 230000005764 inhibitory process Effects 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 229940041514 candida albicans extract Drugs 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 230000002265 prevention Effects 0.000 description 3
- 230000001988 toxicity Effects 0.000 description 3
- 231100000419 toxicity Toxicity 0.000 description 3
- 239000012138 yeast extract Substances 0.000 description 3
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 2
- 241000228197 Aspergillus flavus Species 0.000 description 2
- 241000282414 Homo sapiens Species 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 230000006696 biosynthetic metabolic pathway Effects 0.000 description 2
- 239000013067 intermediate product Substances 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- GRONZTPUWOOUFQ-UHFFFAOYSA-M sodium;methanol;hydroxide Chemical compound [OH-].[Na+].OC GRONZTPUWOOUFQ-UHFFFAOYSA-M 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 description 1
- 108020004465 16S ribosomal RNA Proteins 0.000 description 1
- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 description 1
- 241000228212 Aspergillus Species 0.000 description 1
- 231100000678 Mycotoxin Toxicity 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 description 1
- 235000019270 ammonium chloride Nutrition 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 231100000357 carcinogen Toxicity 0.000 description 1
- 239000003183 carcinogenic agent Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 239000001630 malic acid Substances 0.000 description 1
- 235000011090 malic acid Nutrition 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000002636 mycotoxin Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- LJCNRYVRMXRIQR-OLXYHTOASA-L potassium sodium L-tartrate Chemical compound [Na+].[K+].[O-]C(=O)[C@H](O)[C@@H](O)C([O-])=O LJCNRYVRMXRIQR-OLXYHTOASA-L 0.000 description 1
- 229940074439 potassium sodium tartrate Drugs 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- 235000011006 sodium potassium tartrate Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 1
- 229940038773 trisodium citrate Drugs 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B9/00—Preservation of edible seeds, e.g. cereals
- A23B9/16—Preserving with chemicals
- A23B9/24—Preserving with chemicals in the form of liquids or solids
- A23B9/26—Organic compounds; Microorganisms; Enzymes
- A23B9/28—Microorganisms; Enzymes; Antibiotics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/42—Preservation of non-alcoholic beverages
- A23L2/44—Preservation of non-alcoholic beverages by adding preservatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3571—Microorganisms; Enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Wood Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Ecology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Organic Chemistry (AREA)
- Environmental Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Nutrition Science (AREA)
- Forests & Forestry (AREA)
- General Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Botany (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Toxicology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides a deep sea actinomycete strain capable of inhibiting synthesis of aflatoxin, which is Rhodococcus erythropolis BC14-M2AF-1 strain (Rhodococcus erythropolis BC14-M2AF-1), is separated from sediments in 6105M depth in the Western Pacific ocean, is preserved in Guangdong province collection center of microbial strains, and has the preservation number of GDMCC No. 61239. Fermenting the BC14-M2AF-1 strain in a fresh water M2 culture medium to obtain a fermented cell-free supernatant, wherein the aflatoxin can be inhibited by 100% from the source of an aflatoxin synthesis way; the fermented cell-free supernatant can still inhibit the synthesis of aflatoxin by 100 percent after being treated at the high temperature and the high pressure of 121 ℃ and 103kPa for 30 minutes. The Rhodococcus erythropolis BC14-M2AF-1 strain and the fermentation product thereof can be used for effectively preventing and controlling aflatoxin pollution in field crop cultivation, grain storage, food and beverage processing, feed production and the like.
Description
The technical field is as follows:
the invention relates to the technical field of deep sea biotechnology and biological prevention and control of aflatoxin, in particular to a deep sea actinomycete rhodococcus erythropolis and an effect of the deep sea actinomycete rhodococcus erythropolis in inhibiting aflatoxin.
Background art:
aflatoxin is a mycotoxin classified as a class I carcinogen by the international cancer agency, is mainly produced by aspergillus flavus, aspergillus parasiticus and other aspergillus fungi, can widely pollute grains planted in fields, and also can seriously harm the health of human beings and animals after the human beings and the animals eat foods and feeds polluted by the aflatoxin in the grain storage period and foods and feeds processed by the grains, so that the significant food and feed safety problem to be solved urgently in the world is effectively solved by effectively inhibiting the pollution of the aflatoxin in the grains, the feeds and the like, and the aflatoxin has important practical significance and remarkable economic and social benefits.
Nowadays, land resources are increasingly scarce, all countries in the world shift targets to the ocean, and particularly, the development and utilization of microbial resources in extreme environments such as deep ocean and the like become the focus of attention and competition in the world, and the research and application of the deep ocean microbial resources in the aspect of aflatoxin pollution prevention and control are not common in the world.
The invention content is as follows:
the technical problem to be solved by the invention is to overcome the defects of the prior art, and the adopted technical scheme is as follows: the deep sea actinomycete Rhodococcus erythropolis and the effect of the deep sea actinomycete Rhodococcus erythropolis in inhibiting aflatoxin are characterized by comprising the following steps:
(1) the deep sea actinomycete is Rhodococcus erythropolis BC14-M2AF-1 strain(s) ((R))RhodococcuserythropolisBC14-M2AF-1) and has been deposited at 26.10.2020 at the Guangdong province culture Collection with deposit number GDMCC No. 61239; the storage address is No. 59 building 5 of the Jie No. 100 of the Jie Zhonglu-Jie city, Guangdong province.
(2) The Rhodococcus erythropolis BC14-M2AF-1 strain is separated from sediments in 6105M water depth of the Western Pacific ocean and is obtained by separation through the following method: serially diluting the sediments stored at 4 ℃ by 10 times, coating the sediments on a fresh water M2 culture medium, culturing at a constant temperature of 28 ℃, picking out single colonies in time, and further streaking and purifying to obtain a pure strain BC14-M2 AF-1;
(3) the strain BC14-M2AF-1 is identified as Rhodococcus erythropolis (R) by 16S rRNA gene moleculesRhodococcus erythropolis) A new strain of (a);
(4) the Rhodococcus erythropolis BC14-M2AF-1 strain is inoculated in an M2 culture medium prepared by fresh water, and is subjected to shaking culture for 4-10 days at the temperature of 18-32 ℃, so that an active substance fermentation liquor containing the active substance for efficiently inhibiting the synthesis of the aflatoxin can be obtained, and the cell-free supernatant of the fermentation liquor can inhibit the synthesis of the aflatoxin by 100%.
(5) The active substance in the fermentation liquor of the Rhodococcus erythropolis BC14-M2AF-1 strain has high-temperature high-pressure stability, and the activity is not lost after the treatment for 30 minutes at the high temperature and the high pressure of 121 ℃ and 103 kPa;
(6) the Rhodococcus erythropolis BC14-M2AF-1 strain and the fermentation liquid thereof can be applied to effective prevention and control of aflatoxin pollution in field crop cultivation, grain storage, food and beverage processing, feed production and the like.
Detailed Description
The present invention is further illustrated by the following examples, which are not intended to limit the scope of the invention.
Example 1: fermentation of Rhodococcus erythropolis strain BC14-M2AF-1
Inoculating liquid seeds of Rhodococcus erythropolis BC14-M2AF-1 strain into a liquid culture medium containing 0.5% of sodium acetate, 0.05% of peptone, 0.05% of yeast extract powder, 0.05% of beef extract, 0.05% of glucose, 0.05% of sucrose, 0.05% of soluble starch, 0.005% of trisodium citrate, 0.005% of malic acid, 0.005% of potassium sodium tartrate, 0.1% of ammonium nitrate, 0.02% of ammonium chloride and 100ml of fresh water according to the inoculation amount of 1-8%, culturing for 4-10 days at the temperature of 18-32 ℃ and at the speed of 100-.
Example 2: inhibition effect of cell-free supernatant fermented by Rhodococcus erythropolis BC14-M2AF-1 strain on synthesis of aflatoxin
The supernatant obtained in example 1 was added to the supernatant in a ratio of 2% glucose to 0.5% yeast extract to supplement nutrients, and used as a culture medium for culturing Aspergillus flavus toxin-producing microorganism Aspergillus parasiticus, the control culture medium was the liquid medium obtained in example 1, 2% glucose and 0.5% yeast extract were added to the culture medium, 1% of spore suspension of Aspergillus parasiticus was inoculated to the culture medium, the culture medium was cultured at 28 ℃ for 6 days, mycelia were collected by centrifugation, intermediate products of the aflatoxin biosynthetic pathway in the mycelia were extracted with a methanol-sodium hydroxide solution, and Optical Density (OD) was measured at 560nm560) The toxicity inhibiting rate (%) = (OD of control culture medium) was calculated560OD of cell-free fermentation supernatant560) OD of control culture solution560x 100, and the inhibition rate of cell-free supernatant fermented by the Rhodococcus erythropolis BC14-M2AF-1 strain on the synthesis of aflatoxin reaches 100 percent through determination and calculation.
Example 3: high-temperature high-pressure stability of active substances in fermentation liquor of Rhodococcus erythropolis strain BC14-M2AF-1
The cell-free fermentation supernatant obtained in example 1 was added at 121oC. Treating at 103kPa for 30 min, naturally cooling to room temperature, and comparing the treated fermentation broth, the fermentation broth without temperature treatment, and the controlInoculating 1% spore suspension of Aspergillus parasiticus into the culture solution, culturing at 28 deg.C for 6 days, centrifuging to collect mycelium, extracting intermediate product of aflatoxin biosynthesis pathway in the mycelium with methanol-sodium hydroxide solution, and measuring Optical Density (OD) at 560nm560) The toxicity inhibiting rate (%) = (OD of control culture medium) was calculated560OD of cell-free fermentation supernatant560) OD of control culture solution560x 100, determined and calculated even at 121oC. The fermentation liquor treated at the high temperature and the high pressure of 103kPa for 30 minutes can also have the same inhibition rate of aflatoxin synthesis as the fermentation liquor which is not treated at the temperature, and the inhibition rate of the aflatoxin synthesis reaches 100 percent, which shows that the toxicity inhibiting active substances in the fermentation liquor have high-temperature and high-pressure stability.
Claims (1)
1. A deep sea actinomycete strain separated from sediments in 6105M water depth of the Western Pacific ocean is Rhodococcus erythropolis BC14-M2AF-1 strain (Rhodococcus erythropolis BC14-M2AF-1), which is preserved in Guangdong province collection of microorganisms in 26 months 10 and 2020, and the preservation number is GDMCC number 61239.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011473063.0A CN113186111A (en) | 2020-12-15 | 2020-12-15 | Deep sea actinomycete rhodococcus erythropolis and application thereof in inhibiting aflatoxin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011473063.0A CN113186111A (en) | 2020-12-15 | 2020-12-15 | Deep sea actinomycete rhodococcus erythropolis and application thereof in inhibiting aflatoxin |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113186111A true CN113186111A (en) | 2021-07-30 |
Family
ID=76972874
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011473063.0A Pending CN113186111A (en) | 2020-12-15 | 2020-12-15 | Deep sea actinomycete rhodococcus erythropolis and application thereof in inhibiting aflatoxin |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113186111A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002099142A2 (en) * | 2001-06-01 | 2002-12-12 | Wilhelm Holzapfel | Actinomycetes for breaking down aflatoxin b1, ochratoxin a and/or zearalenone |
| CN104745493A (en) * | 2013-12-27 | 2015-07-01 | 中粮营养健康研究院有限公司 | Separating, culturing and application methods for Rhodococcus erythropolis strain used for degrading aflatoxin B1 |
| CN104738363A (en) * | 2013-12-27 | 2015-07-01 | 中粮营养健康研究院有限公司 | Uses of rhodococcus erythropolis in degradation of aflatoxin B1 in feed or raw materials of the feed |
| CN105039199A (en) * | 2015-06-10 | 2015-11-11 | 哈尔滨工业大学（威海） | Deep-sea Bacillus circulans and application thereof in suppression of aflatoxin |
-
2020
- 2020-12-15 CN CN202011473063.0A patent/CN113186111A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002099142A2 (en) * | 2001-06-01 | 2002-12-12 | Wilhelm Holzapfel | Actinomycetes for breaking down aflatoxin b1, ochratoxin a and/or zearalenone |
| CN104745493A (en) * | 2013-12-27 | 2015-07-01 | 中粮营养健康研究院有限公司 | Separating, culturing and application methods for Rhodococcus erythropolis strain used for degrading aflatoxin B1 |
| CN104738363A (en) * | 2013-12-27 | 2015-07-01 | 中粮营养健康研究院有限公司 | Uses of rhodococcus erythropolis in degradation of aflatoxin B1 in feed or raw materials of the feed |
| CN105039199A (en) * | 2015-06-10 | 2015-11-11 | 哈尔滨工业大学（威海） | Deep-sea Bacillus circulans and application thereof in suppression of aflatoxin |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11242502B2 (en) | Low urea-producing and flavor-producing Wickerhamomyces anomalus strain and use thereof in food production | |
| CN103173397A (en) | Broad-spectrum antibacterial bacillus amyloliquefaciens strain and application thereof | |
| CN108192829B (en) | Streptomyces luteorusand biological control preparation and application thereof | |
| CN108991318B (en) | Seaweed fermented beverage | |
| Dutta et al. | A comparative study of citric acid production from different agro-industrial wastes by Aspergillus niger isolated from mangrove forest soil. | |
| CN102080046A (en) | High-yield laccase strain and method for producing laccase through fermentation | |
| Almousa et al. | Citric acid fermentation by Aspergillus niger | |
| Adedayo et al. | Pectinolytic activity of aspergillus Niger and Aspergillus flavus grown on grapefruit (citrus Parasidis) peel in solid state fermentation | |
| CN113832048B (en) | Bacillus amyloliquefaciens for inhibiting soy sauce from forming white film and application thereof | |
| CN102127515B (en) | Screening and application of L-proline high-producing Brevundimonas sp. (JNPP-1) | |
| CN111471603B (en) | Aroma-producing pichia guilliermondii for producing beta-glucosidase and application thereof | |
| CN110684702B (en) | Bos-Si genus Y4 and application thereof in promoting growth of hypsizigus marmoreus | |
| CN118064296A (en) | Compound microbial agent for inhibiting oyster mushroom bacterial diseases | |
| CN111534453B (en) | Bifidobacterium adolescentis capable of inhibiting filamentous fungi and application thereof | |
| CN109136100B (en) | Aspergillus tubingensis strain and application thereof in fermenting green brick tea | |
| CN116496969A (en) | Method for improving lactic acid tolerance by exogenously adding arginine | |
| CN116574619A (en) | Pichia kudriavzevii and application thereof | |
| CN113186111A (en) | Deep sea actinomycete rhodococcus erythropolis and application thereof in inhibiting aflatoxin | |
| CN113215013A (en) | Difunctional deep sea alzheimer's bacteria for degrading and inhibiting aflatoxin | |
| CN112574915A (en) | Deep sea bacterium eurotium idescens and application thereof in inhibiting aflatoxin | |
| CN112458003B (en) | Diacetyl-producing lactobacillus plantarum and application thereof in pickled vegetables | |
| Abdulameer et al. | Optimum conditions for Inulinase production by Aspergillus niger using solid state fermentation | |
| Siddiqui et al. | Polygalacturonase production from Rhizomucor pusillus isolated from fruit markets of Uttar Pradesh | |
| CN102703479B (en) | 6-geranyl geranyl diphosphate (GGPP) synthase, 8-sesquiterpene synthase and pleuromutilin high-yield strains, and application of strains | |
| CN113322192A (en) | Polygonatum sibiricum endophytic fungi escherichia coli inhibiting agent and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |