CN113184976B - Method for promoting anaerobic granular sludge to load iron-based conductive material and improving methanogenesis efficiency by utilizing biofilm dissociation agent - Google Patents
Method for promoting anaerobic granular sludge to load iron-based conductive material and improving methanogenesis efficiency by utilizing biofilm dissociation agent Download PDFInfo
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Abstract
The invention discloses a method for promoting anaerobic granular sludge to load an iron-based conductive material and improving methanogenesis efficiency by using a biological membrane dissociation agent, belonging to the technical field of anaerobic biological treatment. The specific method comprises the following steps: adding a bis (3-aminopropyl) amine and a D-tyrosine coupling product into the anaerobic granular sludge mixed liquor to serve as a biological membrane dissociation agent, and adding a carbon source to maintain biological metabolic activity; then adding the iron-based conductive material suspension, supplementing a carbon source, and collecting anaerobic granular sludge after reaction to obtain anaerobic granular sludge loaded with the iron-based conductive material; the methane gas is recovered by using the organic wastewater treated by the method. According to the method, the structural pores of the anaerobic granular sludge are increased by using the biomembrane dissociation agent, the load of the iron-based conductive material in the anaerobic granular sludge is realized, the electron transfer process between microorganism direct inoculation is promoted, the organic pollutant degradation capability and the methane production efficiency of the anaerobic granular sludge are obviously improved, and the resource recovery capability in the field of anaerobic biological treatment can be effectively improved.
Description
Technical Field
The invention belongs to the technical field of anaerobic biological treatment, and particularly relates to a method for promoting anaerobic granular sludge to load an iron-based conductive material and improving methanogenesis efficiency by using a biological membrane dissociation agent.
Background
The anaerobic biological treatment technology is a sewage treatment technology which is characterized in that anaerobic and facultative anaerobic microbial floras convert organic matters in sewage into methane, hydrogen and carbon dioxide under anaerobic conditions and synthesize self cell substances. The technology has the characteristics of high organic load, low energy consumption, small amount of generated excess sludge, capability of generating renewable energy sources such as methane (methane and carbon dioxide) and the like. The anaerobic granular sludge has physical, chemical and biological characteristics and high microbial activity, and is a special microbial aggregate formed by aggregation of anaerobic/facultative microbes with different functions. The anaerobic biological treatment technology has the advantages of good sedimentation performance, high biomass concentration, high metabolic activity and the like, thereby being a high-efficiency anaerobic biological treatment technology.
The whole process of anaerobic digestion can be divided into three stages of hydrolytic acidification, hydrogen production and acetogenesis and methanogenesis, and microorganisms participating in the process are various and mainly comprise four populations of hydrolytic acidification bacteria, hydrogen production and acetogenesis bacteria, homoacetogenesis bacteria and methanogenesis bacteria. The hydrogen is a product of hydrolytic acidification bacteria, can be used as a direct electron donor of hydrogenotrophic methanogens to reduce carbon dioxide, and has a high diffusion rate, so that interspecific hydrogen transfer is an important electron transfer mode in a cooperative process of the hydrolytic acidification bacteria and the methanogens, but a bioelectronic link based on the interspecific hydrogen transfer is fragile, and related microorganisms are easily influenced by environmental conditions, so that the partial pressure of the hydrogen in an anaerobic system is increased, the balance of methanogenesis is damaged, and finally the anaerobic methanation is stopped. Recent research shows that the anaerobic system has a more efficient Interspecies Electron and energy Transfer mechanism, namely Direct Interspecies Electron Transfer (DIET) through bioelectronic linkage or non-biological electrochemically active substances. Researches find that the DIET process can be promoted by adding a conductive material in an anaerobic digestion system, and the methanogenesis efficiency is improved.
The anaerobic granular sludge is composed of a multilayer structure, the outer layer is mainly dominated by acidifying bacteria, and the middle layer is used for producing H 2 Bacteria and consume H 2 The bacteria dominate, and the inner layer is composed of methanogen in acetotroph type, which is the core formed initially by the granules. The flora grows mutually, and is aggregated to form granular, complex and compact flora structure, so that in an anaerobic granular sludge system, a method for directly adding a conductive material is difficult to effectively construct DIET channels among microorganisms.
Researches show that D-type amino acid and bis (3-aminopropyl) amine can control the aggregation growth of microorganisms and have good disintegration effect on bacterial biofilms and microorganism aggregates, but researches on whether the D-type amino acid and the bis (3-aminopropyl) amine can be applied to anaerobic methanogenesis have not been reported. Therefore, the invention provides a method for regulating and controlling the structure of the anaerobic granular sludge by utilizing the biomembrane dissociation agent so as to better introduce a conductive material, strengthen the DIET process and further improve the methane production capability of the anaerobic granular sludge.
Disclosure of Invention
In order to solve the problems, the invention provides a method for improving methanogenesis efficiency by promoting anaerobic granular sludge to load an iron-based conductive material by using a biological membrane dissociation agent, which comprises the following steps:
1) The structure of the anaerobic granular sludge is regulated and controlled by utilizing a biomembrane dissociating agent: adding a biological membrane to the anaerobic granular sludge to dissociate and regulate the anaerobic granular sludge so as to dissociate extracellular polymer amount of the anaerobic granular sludge, thereby increasing structural pores of the anaerobic granular sludge, and adding a carbon source to maintain the biological metabolism activity of the anaerobic granules; wherein, the coupling of the bis (3-aminopropyl) amine and the D-tyrosine is used as a biological membrane dissociation agent;
2) Anaerobic granular sludge loaded iron-based conductive material: adding a freshly prepared iron-based conductive material suspension into the anaerobic granular sludge system treated in the step 1), and supplementing a carbon source to form an anaerobic granular sludge loaded iron-based conductive material reaction system; anaerobic granular sludge is collected after the reaction to obtain anaerobic granular sludge loaded with the iron-based conductive material;
3) The method comprises the following steps of (1) treating organic wastewater by using anaerobic granular sludge loaded with iron-based conductive materials to recover methane gas: adding the anaerobic granular sludge loaded with the iron-based conductive material in the step 2) into an anaerobic reactor, regulating and controlling the nutrient elements of nitrogen and phosphorus in the inlet water to meet the basic growth requirements of anaerobic microorganisms, collecting the generated gas, and measuring the content of methane.
The anaerobic granular sludge is obtained from a sewage treatment plant.
The concentration of the biomembrane dissociation agent in the step 1) is 0.5 mM-5 mM, the molar ratio of the bis (3-aminopropyl) amine to the D-tyrosine is 0.5-1, and the dissociation reaction time is 1-6 h;
the iron-based conductive material in the step 2) comprises nano zero-valent iron ZVI and nano Fe 2 O 3 Nano Fe 3 O 4 One or more of them.
In the step 2), the concentration of the iron-based conductive material suspension is 100-2000 mg/L, and the biomass ratio of Fe/granular sludge is controlled to be (1-200): 100, respectively; based on the content of Fe element, the final load capacity of the iron-based conductive material in the anaerobic granular sludge is 22-200 mg Fe/g VSS.
Adding an inorganic salt culture solution and a carbon source into the anaerobic granular sludge in the step 1) to prepare an anaerobic granular sludge mixed solution; the concentration of the anaerobic granular sludge in the anaerobic granular sludge mixed liquor is 1-8 gMLVSS/L.
The inorganic salt culture solution comprises the following components: NH 4 HCO 3 ＝360mg/L、KH 2 PO 4 ＝60mg/L、NaHCO 3 ＝1000mg/L、CaCl 2 =7.5mg/L, trace element solution 1mL/L; the trace element solution comprises the following components: niSO 4 ·6H 2 O＝500mg/L、MnCl 2 ·4H 2 O＝500mg/L、FeSO 4 ·7H 2 O＝500mg/L、ZnSO 4 ·7H 2 O＝100mg/L、H 3 BO 3 ＝100mg/L、Na 2 MoO 4 ·2H 2 O＝50mg/L、CoCl 2 ·6H 2 O＝50mg/L、CuSO 4 ·5H 2 O＝5mg/L。
The carbon source is one or a combination of more of glucose, sucrose and acetate, and the concentration is 2000-3000 mg/L.
The anaerobic granular sludge needs to be pretreated, and the method comprises the following specific steps: and washing the anaerobic granular sludge by using an inorganic salt culture solution to remove organic pollutants on the surface of the anaerobic granular sludge, and centrifugally collecting the anaerobic granular sludge for later use.
In the step 3), the concentration of anaerobic granular sludge is controlled to be 5-20 g MLVSS/L, the concentration of easily degradable organic matters in inlet water (COD meter) is controlled to be 800-8000 mg/L, and the ratio of C to N to P = 200.
The anaerobic condition is maintained in the reaction processes of the steps 1) to 3), oxygen in a system is removed by blowing nitrogen, and the anaerobic condition is maintained by sealing; the reaction system is placed in a constant temperature shaking table to avoid light and shake reaction, the reaction temperature is 20-40 ℃, and the rotation speed of the shaking table is 100-150 rpm.
The oscillation reaction time in the step 2) is 12-24 h.
The invention has the beneficial effects that:
1. a biomembrane dissociating agent formed by coupling bis (3-aminopropyl) amine and D-tyrosine acts on the anaerobic granular sludge to moderately dissociate Extracellular Polymeric Substance (EPS) quantity, so that the density of extracellular polysaccharide and extracellular protein is reduced, the three-dimensional structure pores of the anaerobic granular sludge are increased, and the internal structure of the anaerobic granular sludge is relatively loose; by adding the iron-based conductive material, the iron-based conductive material can better enter the anaerobic granular sludge from the pores to mediate the iron-based conductive material, so that the direct interspecific electron transfer among hydrogen-producing acetogenic bacteria, hydrolytic zymogens and methanogens in the anaerobic granular sludge is promoted, and the capability of degrading organic pollutants and the efficiency of producing methane in the anaerobic granular sludge are obviously improved.
2. The method makes full use of different types of iron-based conductive materials including nano zero-valent iron (ZVI) and nano Fe 2 O 3 Nano Fe 3 O 4 And the sludge can enter the anaerobic granular sludge to promote the direct inter-inoculation electron transfer process of an anaerobic digestion system.
3. The application utilizes an anaerobic biological treatment technology, and adopts bis (3-aminopropyl) amine and a D-tyrosine coupled biomembrane dissociating agent to act on the aggregation anaerobic granular sludge; anaerobic granular sludge serves as a complex mixed flora aggregate and comprises a plurality of microbial communities such as gram-positive bacteria and gram-negative bacteria, and the combination of the bis (3-aminopropyl) amine and the D-tyrosine plays a role in inhibiting the synthesis and secretion of extracellular matrixes in sludge granules in a complementary mode, so that the complex microbial aggregate is depolymerized.
4. The method for promoting the anaerobic granular sludge to load the iron-based conductive material by using the biofilm dissociation agent is simple to operate and environment-friendly, and can effectively improve the resource recovery capability in the field of anaerobic biological treatment.
Drawings
FIG. 1 is the example 2 supporting nano Fe 2 O 3 An anaerobic granular sludge methane yield map of the iron-based conductive material;
FIG. 2 is the example 3 supporting nano Fe 2 O 3 An anaerobic granular sludge methane yield map of the iron-based conductive material;
FIG. 3 is a graph of the effect of the addition of 1000mg/L of nano zero-valent iron, and the absence and addition of biofilm dissociation agents on the iron loading of anaerobic granular sludge in example 4.
Detailed Description
The invention is described in further detail below with reference to the following figures and specific examples:
example 1
Preparing an inorganic salt culture solution according to the requirements, wherein the inorganic salt culture solution comprises the following components in percentage by weight: NH (NH) 4 HCO 3 ＝360mg/L、KH 2 PO 4 ＝60mg/L、NaHCO 3 ＝1000mg/L、CaCl 2 =7.5mg/L, trace element solution 1mL/L; the trace element solution comprises the following components: niSO 4 ·6H 2 O＝500mg/L、MnCl 2 ·4H 2 O＝500mg/L、FeSO 4 ·7H 2 O＝500mg/L、ZnSO 4 ·7H 2 O＝100mg/L、H 3 BO 3 ＝100mg/L、Na 2 MoO 4 ·2H 2 O＝50mg/L、CoCl 2 ·6H 2 O＝50mg/L、CuSO 4 ·5H 2 O＝5mg/L；
Anaerobic granular sludge of a sewage treatment plant is taken and washed by Phosphate Buffer Solution (PBS), prepared inorganic salt culture solution is added, glucose is added as a carbon source, and the COD of the system is adjusted: n: p =200:5:1; placing the anaerobic granular sludge mixed liquor in a headspace bottle, and preparing the anaerobic granular sludge mixed liquor to ensure that the sludge concentration is 6g MLVSS/L.
The method for promoting anaerobic granular sludge to load the iron-based conductive material to improve the methanogenesis efficiency by utilizing the biomembrane dissociation agent comprises the following steps:
1) Adding 0.5mM of bis (3-aminopropyl) amine and a D-tyrosine coupling biomembrane dissociating agent into the prepared anaerobic granular sludge mixed solution, taking 2000mg/L of glucose as a carbon source, and adjusting the pH value of the system to be 7; sealing after blowing nitrogen for 15min, and culturing in constant temperature shaking table at 35 deg.C and 150rpm for 6h in dark place for dissociation; wherein the molar ratio of the bis (3-aminopropyl) amine to the D-tyrosine is 1;
2) After precipitation, the supernatant is poured out, and 100mg/L of freshly prepared nano Fe is added 3 O 4 And (2) controlling the Fe/biomass ratio to be 1:70; supplementing inorganic salt culture solution and 2000mg/L glucose as carbon source; sealing after nitrogen blowing for 15min, and culturing for 24h in a constant temperature shaking table at 35 ℃ and 150rpm in a dark place to obtain the anaerobic granular sludge loaded with the iron-based conductive material, wherein the iron content in the anaerobic granular sludge per dry weight is 22.34mg Fe/g VSS;
3) Washing the anaerobic granular sludge loaded with the iron-based conductive material for three times, adding the washed anaerobic granular sludge into an anaerobic reactor, enabling the sludge concentration to be 8g MLVSS/L, enabling the inflow water to be 2500mg/L glucose to simulate waste water, regulating and controlling other nutrient elements such as inflow water nitrogen, phosphorus and the like, and keeping C: n: p =200, 1, meeting basic requirements for anaerobic microorganism growth, sealing the reactor to maintain anaerobic conditions, reacting at 35 ℃, collecting produced gas during the reaction, and measuring the methane content in the gas.
Compared with anaerobic granular sludge without loading the iron-based conductive material, the methane cumulative yield of the anaerobic granular sludge directly loaded with the iron-based conductive material without adding the biofilm dissociation agent and loaded with the iron-based conductive material with adding the biofilm dissociation agent is respectively improved by 8.39 percent and 15.76 percent.
Example 2
Taking anaerobic granular sludge of a sewage treatment plant, cleaning the anaerobic granular sludge by Phosphate Buffer Solution (PBS), adding inorganic salt culture solution, taking glucose as a carbon source, and adjusting the COD of the system: n: p =200:5:1; placing the anaerobic granular sludge mixed liquor in a headspace bottle, and preparing the anaerobic granular sludge mixed liquor to ensure that the sludge concentration is 6g MLVSS/L.
The method for improving the methanogenesis efficiency by promoting anaerobic granular sludge to load iron-based conductive materials by utilizing the biological membrane dissociation agent comprises the following steps:
1) Adding 3mM bis (3-aminopropyl) amine and a D-tyrosine coupling biomembrane dissociation agent into the prepared anaerobic granular sludge mixed solution, using 2000mg/L glucose as a carbon source, and adjusting the pH value of the system to be 7; sealing after blowing nitrogen for 15min, and culturing in constant temperature shaking table at 35 deg.C and 150rpm for 6h in dark place; wherein the molar ratio of bis (3-aminopropyl) amine to D-tyrosine is 1;
2) After precipitation, the supernatant is poured out, and 500mg/L of freshly prepared nano Fe is added 2 O 3 And (2) controlling the Fe/biomass ratio to be 1:14; supplementing inorganic salt culture solution, and using 2000mg/L glucose as a carbon source; sealing after nitrogen blowing for 15min, and culturing in a constant temperature shaking table at 35 ℃ and 150rpm in a dark place for 24h to obtain anaerobic granular sludge loaded with the iron-based conductive material, wherein the iron content in the anaerobic granular sludge per unit dry weight is 32.08mg Fe/g VSS;
3) Washing the anaerobic granular sludge loaded with the iron-based conductive material for three times, adding the washed anaerobic granular sludge into an anaerobic reactor to enable the sludge concentration to be 8g MLVSS/L and the inlet water to be 2500mg/L glucose simulation wastewater, regulating and controlling other nutrient elements such as inlet water nitrogen, phosphorus and the like to meet the basic growth requirements of anaerobic microorganisms, sealing the reactor to maintain anaerobic conditions, reacting at the temperature of 35 ℃, collecting generated gas in the reaction process, and measuring the methane content in the gas.
With reference to the above examples, sodium acetate is used as an electron donor, a biofilm dissociation agent is added, and nano-Fe is loaded 2 O 3 Iron-based conductive material and non-loaded nano Fe 2 O 3 A comparison graph of the methane yield of anaerobic granular sludge with iron-based conductive material directly loaded without adding a biofilm dissociation agent is shown in FIG. 1. Compared with anaerobic granular sludge without loading the iron-based conductive material, the methane cumulative yield of the anaerobic granular sludge directly loaded with the iron-based conductive material without adding the biofilm dissociation agent and loaded with the iron-based conductive material with adding the biofilm dissociation agent is respectively improved by 35.97% and 56.14%.
Example 3
Taking anaerobic granular sludge of a sewage treatment plant, cleaning the anaerobic granular sludge by Phosphate Buffer Solution (PBS), adding inorganic salt culture solution, taking glucose as a carbon source, and adjusting the COD of the system: n: p =200:5:1; placing the anaerobic granular sludge mixed liquor in a headspace bottle, and preparing the anaerobic granular sludge mixed liquor to ensure that the sludge concentration is 6g MLVSS/L.
The method for promoting anaerobic granular sludge to load the iron-based conductive material to improve the methanogenesis efficiency by utilizing the biomembrane dissociation agent comprises the following steps:
1) Adding 3mM bis (3-aminopropyl) amine and a D-tyrosine coupling biomembrane dissociating agent into the prepared anaerobic granular sludge mixed solution, taking 2000mg/L glucose as a carbon source, and adjusting the pH value of the system to be 7; sealing after blowing nitrogen for 15min, and culturing for 6h in a constant temperature shaking table at 35 ℃ and 150rpm in the dark; wherein the molar ratio of bis (3-aminopropyl) amine to D-tyrosine is 1;
2) After precipitation, the supernatant is poured out, and 1000mg/L of freshly prepared nano Fe is added 2 O 3 And (2) controlling the Fe/biomass ratio to be 1:6; supplementing inorganic salt culture solution, and using 2000mg/L glucose as a carbon source; blowing nitrogen for 15min, sealing, and culturing in constant temperature shaking table at 35 deg.C and 150rpm for 24 hr in dark place to obtain negativeAnaerobic granular sludge loaded with iron-based conductive materials, wherein the iron content in the anaerobic granular sludge per dry weight is 47.18mg Fe/g VSS;
3) Washing the anaerobic granular sludge loaded with the iron-based conductive material for three times, adding the washed anaerobic granular sludge into an anaerobic reactor to enable the sludge concentration to be 8g MLVSS/L and the inlet water to be 2500mg/L glucose simulation wastewater, regulating and controlling other nutrient elements such as inlet water nitrogen, phosphorus and the like to meet the basic growth requirements of anaerobic microorganisms, sealing the reactor to maintain anaerobic conditions, reacting at the temperature of 35 ℃, collecting generated gas in the reaction process, and measuring the methane content in the gas.
In combination with the above embodiment, sodium acetate is used as an electron donor, a biofilm dissociation agent is added, and nano Fe is loaded 2 O 3 Iron-based conductive material and non-loaded nano Fe 2 O 3 A comparison graph of the methane yield of the anaerobic granular sludge with the iron-based conductive material directly loaded without the addition of the biofilm dissociation agent is shown in FIG. 2. Compared with anaerobic granular sludge without loading the iron-based conductive material, the methane cumulative yield of the anaerobic granular sludge directly loaded with the iron-based conductive material without adding the biofilm dissociation agent and loaded with the iron-based conductive material with adding the biofilm dissociation agent is respectively improved by 9.39% and 28.23%.
Example 4
Taking anaerobic granular sludge of a sewage treatment plant, cleaning the anaerobic granular sludge by using Phosphate Buffer Solution (PBS), adding inorganic salt culture solution, taking glucose as a carbon source, and adjusting the COD: n: p =200:5:1; placing the anaerobic granular sludge mixed liquor in a headspace bottle to prepare anaerobic granular sludge mixed liquor, wherein the sludge concentration is 6g MLVSS/L.
The method for promoting anaerobic granular sludge to load the iron-based conductive material to improve the methanogenesis efficiency by utilizing the biomembrane dissociation agent comprises the following steps:
1) Adding 3mM bis (3-aminopropyl) amine and a D-tyrosine coupling biological membrane dissociation agent into the anaerobic granular sludge mixed liquor; 2000mg/L glucose is used as a carbon source, and the pH value of the system is adjusted to 7; sealing after blowing nitrogen for 15min, and culturing in constant temperature shaking table at 35 deg.C and 150rpm for 6h in dark place;
2) And after precipitation, pouring out supernatant, adding 1000mg/L of freshly prepared nano zero-valent iron (ZVI) iron-based conductive material suspension, and controlling the Fe/biomass ratio to be 1:6; supplementing inorganic salt culture solution, and using 2000mg/L glucose as a carbon source; sealing after nitrogen blowing for 15min, and culturing for 24h in a constant temperature shaking table at 35 ℃ and 150rpm in a dark place to obtain anaerobic granular sludge loaded with the iron-based conductive material;
3) Washing the anaerobic granular sludge loaded with the iron-based conductive material for three times, adding the washed anaerobic granular sludge into an anaerobic reactor to enable the sludge concentration to be 8g MLVSS/L and the inlet water to be 2500mg/L glucose simulation wastewater, regulating and controlling other nutrient elements such as inlet water nitrogen, phosphorus and the like to meet the basic growth requirements of anaerobic microorganisms, sealing the reactor to maintain anaerobic conditions, reacting at the temperature of 35 ℃, collecting generated gas in the reaction process, and measuring the methane content in the gas.
Compared with anaerobic granular sludge without loading the iron-based conductive material, the methane cumulative yield of the anaerobic granular sludge directly loaded with the iron-based conductive material without adding the biofilm dissociation agent and loaded with the iron-based conductive material with adding the biofilm dissociation agent is respectively improved by 15.51 percent and 33.46 percent.
After loading the iron-based conductive material in the step 2), further measuring the iron content in the granular sludge by using an inductively coupled plasma emission spectrometer. The iron contents of the anaerobic granular sludge in which the iron-based conductive material was not supported, the iron-based conductive material was directly supported without addition of the biofilm dissociation agent, and the iron-based conductive material was supported with addition of the biofilm dissociation agent are shown in fig. 3, and it can be seen from the results that the iron contents of the anaerobic granular sludge per dry weight were 15.71mg Fe/g VSS, 41.63mg Fe/g VSS, and 68.76mg Fe/g VSS, respectively. The addition of the biofilm dissociation agent is shown to improve the iron loading of the anaerobic granular sludge.
Claims (8)
1. A method for promoting anaerobic granular sludge to load an iron-based conductive material to improve methanogenesis efficiency by utilizing a biological membrane dissociation agent is characterized by comprising the following steps of: the method comprises the following steps:
1) The structure of the anaerobic granular sludge is regulated and controlled by utilizing a biomembrane dissociating agent: adding a biological membrane dissociation agent into the anaerobic granular sludge mixed liquor to regulate and control the anaerobic granular sludge and dissociate extracellular polymers thereof, so that structural pores of the anaerobic granular sludge are increased, and adding a carbon source to maintain the biological metabolic activity of the anaerobic granules; wherein, the coupling of the bis (3-aminopropyl) amine and the D-tyrosine is used as a biological membrane dissociating agent;
2) Anaerobic granular sludge loaded iron-based conductive material: adding the iron-based conductive material suspension into the anaerobic granular sludge system treated in the step 1), and supplementing a carbon source to form an anaerobic granular sludge loaded iron-based conductive material reaction system; anaerobic granular sludge is collected after reaction, and the anaerobic granular sludge loaded with the iron-based conductive material is obtained; the iron-based conductive material comprises nano zero-valent iron ZVI and nano Fe 2 O 3 One or more of the above;
the concentration of the iron-based conductive material suspension is 100-2000 mg/L, and the biomass ratio of Fe/granular sludge is controlled to be (1-200): 100, the final load capacity of the iron-based conductive material in the anaerobic granular sludge is 22 to 200mg Fe/g VSS;
3) The method comprises the following steps of (1) treating organic wastewater by using anaerobic granular sludge loaded with iron-based conductive materials to recover methane gas: adding the anaerobic granular sludge loaded with the iron-based conductive material in the step 2) into an anaerobic reactor, regulating and controlling the nutrient elements of nitrogen and phosphorus in the inlet water to meet the basic growth requirement of anaerobic microorganisms, collecting the generated gas, and measuring the content of methane;
the concentration of the biofilm dissociation agent in the step 1) is 0.5 to 5mM, the molar ratio of the bis (3-aminopropyl) amine to the D-tyrosine is 0.5 to 1, and the dissociation reaction time is 1 to 6 hours.
2. The method for promoting the anaerobic granular sludge to load the iron-based conductive material to improve the methanogenic efficiency by using the biofilm dissociation agent as claimed in claim 1, wherein the biofilm dissociation agent comprises the following steps: the iron-based conductive material in the step 2) also comprises nano Fe 3 O 4 。
3. The method for promoting the anaerobic granular sludge to load the iron-based conductive material to improve the methanogenic efficiency by using the biofilm dissociation agent as claimed in claim 1, wherein the biofilm dissociation agent comprises the following steps: adding an inorganic salt culture solution and a carbon source into anaerobic granular sludge in the step 1) to prepare an anaerobic granular sludge mixed solution; the concentration of the anaerobic granular sludge in the anaerobic granular sludge mixed liquor is 1 to 8g MLVSS/L.
4. The method for promoting the anaerobic granular sludge to load the iron-based conductive material to improve the methanogenic efficiency by using the biofilm dissociation agent as claimed in claim 3, wherein the biofilm dissociation agent comprises the following steps: the inorganic salt culture solution comprises the following components: NH 4 HCO 3 =360mg/L、 KH 2 PO 4 =60mg/L、NaHCO 3 =1000mg/L、CaCl 2 =7.5mg/L, trace element solution 1mL/L; the trace element solution had the following composition: niSO 4 ·6H 2 O=500mg/L、MnCl 2 ·4H 2 O=500mg/L、FeSO 4 ·7H 2 O=500mg/L、ZnSO 4 ·7H 2 O=100mg/L、H 3 BO 3 =100mg/L 、 Na 2 MoO 4 ·2H 2 O=50mg/L、CoCl 2 ·6H 2 O=50mg/L、CuSO 4 ·5H 2 O=5mg/L。
5. The method for promoting the anaerobic granular sludge to load the iron-based conductive material to improve the methanogenic efficiency by using the biofilm dissociation agent as claimed in claim 3, wherein the biofilm dissociation agent comprises the following steps: the carbon source is one or a combination of more of glucose, sucrose and acetate, and the concentration is 2000 to 3000mg/L.
6. The method for promoting the anaerobic granular sludge to load the iron-based conductive material to improve the methanogenic efficiency by using the biofilm dissociation agent as claimed in claim 1, wherein the biofilm dissociation agent comprises the following steps: controlling the concentration of the anaerobic granular sludge in the step 3) to be 5-20g MLVSS/L; controlling the concentration of easily degradable organic matters in the inlet water to be 800 to 8000mg/L in terms of COD; c: n: p = 200.
7. The method for promoting the anaerobic granular sludge to load the iron-based conductive material to improve the methanogenic efficiency by using the biofilm dissociation agent as claimed in claim 1, wherein the biofilm dissociation agent comprises the following steps: the anaerobic condition is maintained in the reaction processes of the step 1) to the step 3), oxygen in a system is removed by blowing nitrogen, and the anaerobic condition is maintained by sealing; placing the reaction system in a constant-temperature shaking table to carry out vibration reaction in a dark place, wherein the reaction temperature is 20-40 ℃, and the rotation speed of the shaking table is 100-150 rpm.
8. The method for promoting the anaerobic granular sludge to load the iron-based conductive material to improve the methanogenic efficiency by using the biofilm dissociation agent as claimed in claim 7, wherein the biofilm dissociation agent comprises the following steps: the oscillation reaction time in the step 2) is 12 to 24h.
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