CN113184357B

CN113184357B - Kit for activating squamous carcinoma specific immune response

Info

Publication number: CN113184357B
Application number: CN202110295896.0A
Authority: CN
Inventors: 李研; 辛竹
Original assignee: First Affiliated Hospital of Dalian Medical University
Current assignee: First Affiliated Hospital of Dalian Medical University
Priority date: 2021-03-19
Filing date: 2021-03-19
Publication date: 2022-11-04
Anticipated expiration: 2041-03-19
Also published as: CN113184357A

Abstract

The invention discloses a kit for activating squamous cell carcinoma specific immunoreaction, which comprises a reagent box body, a temperature-insulating sleeve, a reagent frame, a clamping mechanism and a refrigerating tube, wherein the temperature-insulating sleeve is sleeved outside the reagent box body, a damping sponge cushion is fixed at the bottom of the reagent box body, the reagent frame is arranged inside the reagent box body above the damping sponge cushion, the outer walls of two sides of the reagent frame are fixedly connected with the inner wall of the reagent box body, positioning jacks are arranged at the corner positions of the top end of the reagent box body, equally-spaced reagent jacks are arranged inside the reagent frame, and the clamping mechanism is arranged inside the reagent jacks.

Description

Kit for activating squamous carcinoma specific immune response

Technical Field

The invention relates to the technical field of kits, in particular to a kit for activating squamous carcinoma specific immunoreaction.

Background

Squamous cell carcinoma is called squamous cell carcinoma for short, also called epidermoid carcinoma, and is a malignant tumor which occurs in epidermis or accessory cells, cancer cells have different degrees of keratinization, are mostly found in parts covered by squamous epithelium, such as skin, oral cavity, lip, esophagus, cervix, vagina and the like, and need to use corresponding reagents in the process of activating squamous cell carcinoma specific immune response, and the reagents are all scattered generally, so the reagents need to be stored by using a kit.

The kit comprises: the box is used for containing chemical reagents for detecting chemical components, drug residues, virus types and the like. Generally used by hospitals and pharmaceutical enterprises. The current RNA extraction kit produced by QIANGEN company has good extraction effect, but has higher selling price, too large expense of single experiment, unnecessary purchasing of experiment with low precision requirement, and other imported kits, but has the problems of high price and long order cycle (if the RNA extraction kit is shipped from abroad when no goods are available in China, the RNA extraction kit can wait for a long time). The kit for ordinary extraction experiments is enough, has low price, is in stock in all regions basically, such as Tiangen (one of the kits produced in China at present and having a wide sale range), has much lower price than imported kits, has good effect and can achieve cost performance.

The existing kits on the market are various in types, can basically meet the use requirements of people, but still have certain defects, and have the following specific problems:

(1) The existing reagent kit is inconvenient to clamp and fix the reagent tube when in use, and the reagent tube is easy to topple, so that inconvenience is brought to people;

(2) When the existing kit is used, the reagent tube in the existing kit is easily influenced by the external environment temperature to cause reagent deterioration, so that certain defects exist;

(3) The existing kit is generally in an open structure when in use, and is easy to fall ash, so that improvement is needed.

Disclosure of Invention

The invention aims to provide a kit for activating a squamous cell carcinoma specific immune response, and aims to solve the problems that a reagent tube is inconvenient to clamp and fix when the kit is used, the reagent tube is easily influenced by the external environment temperature and the ash is easily dropped in an open structure in the background technology.

In order to achieve the purpose, the invention provides the following technical scheme: the utility model provides a kit for activating squamous carcinoma specific immunoreaction, includes reagent box body, separates temperature cover, reagent frame, fixture and refrigeration pipe, the outside cover of reagent box body is equipped with separates the temperature cover, and the bottom of reagent box body is fixed with the shock attenuation foam-rubber cushion, the inside reagent frame that is provided with of reagent box body of shock attenuation foam-rubber cushion top, the outer wall of reagent frame both sides all with reagent box body's inner wall fixed connection, the turning position department on reagent box body top all is provided with the location jack, the inside of reagent frame all is provided with equidistant reagent jack, and reagent jack's inside is provided with fixture, the equal fixedly connected with support column in four corners of bottom, four the equal fixedly connected with slipmat in bottom, the fixed a plurality of spliced poles that are provided with of inside, the equal fixedly connected with protection mat in both sides.

Preferably, be provided with the refrigeration chamber in the lateral wall of reagent box body bottom, and the refrigeration pipe of equidistant all installing in the inside in refrigeration chamber is convenient for reduce the inside temperature of reagent box body, provides the best storage environment of a temperature for the reagent pipe.

Preferably, the top of reagent box body is provided with the shield, and the inside of shield is provided with the cavity, the inside fixed mounting of cavity has a plurality of dust tubes, can strengthen whole reagent box body's sealed, is convenient for seal reagent box body for the dust can not enter into reagent box body's inside.

Preferably, a temperature sensor is installed on the inner wall of the reagent box body above the reagent rack, and a detection probe is installed on the surface of the temperature sensor, so that the temperature inside the reagent box body can be conveniently detected, and the storage of the reagent is prevented from being influenced by overhigh temperature.

Preferably, the top of shield is provided with the handle, the bottom of handle and the top fixed connection of shield, the handheld cover of top fixedly connected with of handle, a plurality of anti-skidding lines have been seted up on the surface of handheld cover, the equal fixedly connected with protection frame in two corners of shield is convenient for carry and is carried the shield, also is difficult for taking place the smooth phenomenon of hand when carrying the shield simultaneously, prevents the unexpected condition.

Preferably, the corner position department of shield bottom all is fixed with the location inserted bar, and the bottom of location inserted bar extends to the inside of location jack, is convenient for fix the shield on the top of reagent box body.

Preferably, the maximum diameter of the positioning insertion rod is smaller than the minimum diameter of the positioning insertion hole, and the maximum length of the positioning insertion rod is smaller than the minimum length of the positioning insertion hole, so that the positioning insertion rod can be completely inserted into the positioning insertion hole.

Preferably, the inside of fixture has set gradually drive chamber, lever, push rod, tray, clamping jaw, actuating lever and supporting spring, and the inside drive chamber that is provided with of reagent frame of reagent jack one side, and the lever is installed to the bottom in drive chamber, and the inside tray that is provided with of reagent jack above the lever, the top swing joint of tray has the clamping jaw to the bottom mounting of tray has the push rod, and the bottom of push rod and one side swing joint on lever top are convenient for carry out the centre gripping fixedly to the reagent pipe.

Preferably, a supporting spring is fixed on one side of the bottom end of the lever, the bottom end of the supporting spring is fixedly connected with the inner wall of the bottom of the driving cavity, a driving rod is movably connected to the top end of the lever at the position of the supporting spring, and the top end of the driving rod extends to the position above the reagent rack, so that elastic supporting force is provided for the lever, and the state of the lever can be changed conveniently.

Compared with the prior art, the invention has the beneficial effects that: the kit for activating the squamous carcinoma specific immunoreaction is convenient for clamping and fixing the reagent tube, so that the reagent is not easily influenced by the external environment, the deterioration of the reagent is reduced, the entering of dust is reduced, the whole kit can be stably placed on a table top, the relative sliding is not easy to generate, and the whole kit also has a protection structure and is not easy to damage;

(1) The reagent tube clamping device is provided with a driving cavity, a lever, a push rod, a tray, clamping jaws, a driving rod and a supporting spring, the driving rod is pressed to move downwards, the driving rod drives the lever to enable the lever to be in an inclined state, the supporting spring is compressed by one end of the lever, meanwhile, the tray inside a reagent jack is lifted by the other end of the lever through the push rod, the tray moves upwards, the clamping jaws at the top end of the tray are opened, then, a reagent tube is inserted into the reagent jack, the driving rod is loosened, the lever is enabled to be restored to a horizontal state under the elastic reset action of the supporting spring, at the moment, the tray drives the clamping jaws to retract into the inside of the reagent jack, and under the limiting action of the reagent jack, the clamping jaws are folded and clamp the reagent tube, so that the reagent tube is clamped and fixed conveniently, and the toppling of the reagent tube is reduced;

(2) The temperature sensor detects the temperature inside the reagent box body through the detection probe, when the temperature inside the reagent box body exceeds the optimal temperature for storing the reagent tube, the cooling tube inside the cooling cavity cools the inside of the reagent box body, and the temperature insulation sleeve sleeved outside the reagent box body separates heat exchange inside and outside the reagent box body, so that the reagent is not easily influenced by the external environment, and the deterioration of the reagent is reduced;

(3) Through being provided with the shield, the handle, the cavity, location inserted bar and location jack, grip the handle and cover the top at reagent box body with the shield, make the location inserted bar card of shield bottom go into inside the location jack on reagent box body top, location inserted bar and location jack cooperation fix the shield, the inside cavity of shield also provides sufficient space for depositing of reagent pipe, the setting up of shield makes the reagent box body be encapsulated situation, thereby the entering of dust has been reduced.

Drawings

FIG. 1 is a schematic front sectional view of the present invention;

FIG. 2 is a schematic top view of the reagent cartridge of the present invention;

FIG. 3 is a schematic bottom view of the dust cap of the present invention;

FIG. 4 is an enlarged schematic view of the structure at A in FIG. 1 according to the present invention;

FIG. 5 is a schematic side wall sectional structure of the kit according to the present invention;

fig. 6 is an enlarged schematic structural view of the clamping mechanism of the present invention.

In the figure: 1. a reagent cartridge body; 2. a thermal insulation sleeve; 3. a shock absorbing sponge cushion; 4. a reagent rack; 5. a dust cover; 6. a handle; 7. a temperature sensor; 8. detecting a probe; 9. a reagent receptacle; 10. a clamping mechanism; 1001. A drive chamber; 1002. a lever; 1003. a push rod; 1004. a tray; 1005. a clamping jaw; 1006. a drive rod; 1007. a support spring; 11. positioning the jack; 12. a cavity; 13. positioning the inserted rod; 14. a refrigeration cavity; 15. a refrigeration pipe; 16. a hand-held sleeve; 17. a protective pad; 18. a support pillar; 19. a protective frame; 20. a dust-proof pipe; 21. connecting columns; 22. provided is a non-slip mat.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-6, an embodiment of the present invention is shown: a kit for activating squamous carcinoma specific immunoreaction comprises a reagent box body 1, a heat insulation sleeve 2, a reagent rack 4, a clamping mechanism 10 and a refrigerating pipe 15, wherein the heat insulation sleeve 2 is sleeved outside the reagent box body 1, and a damping spongy cushion 3 is fixed at the bottom of the reagent box body 1;

a refrigeration cavity 14 is arranged in the side wall of the bottom of the reagent box body 1, and refrigeration pipes 15 with equal intervals are arranged in the refrigeration cavity 14, so that the temperature in the reagent box body 1 can be conveniently reduced, and a storage environment with the optimal temperature is provided for the reagent pipes;

the top end of the reagent box body 1 is provided with a dustproof cover 5, a cavity 12 is arranged inside the dustproof cover 5, and a plurality of dustproof pipes 20 are fixedly arranged inside the cavity 12, so that the reagent box body 1 is convenient to seal, and dust cannot enter the inside of the reagent box body 1;

a lifting handle 6 is arranged at the top end of the dustproof cover 5, and the bottom end of the lifting handle 6 is fixedly connected with the top end of the dustproof cover 5, so that the dustproof cover 5 can be conveniently lifted;

the corner positions of the bottom end of the dustproof cover 5 are all fixed with positioning insertion rods 13, and the bottom ends of the positioning insertion rods 13 extend into the positioning insertion holes 11, so that the dustproof cover 5 is conveniently fixed at the top end of the reagent box body 1;

the maximum diameter of the positioning insertion rod 13 is smaller than the minimum diameter of the positioning insertion hole 11, and the maximum length of the positioning insertion rod 13 is smaller than the minimum length of the positioning insertion hole 11, so that the positioning insertion rod 13 can be completely inserted into the positioning insertion hole 11;

a reagent rack 4 is arranged in the reagent box body 1 above the damping sponge cushion 3, the outer walls of two sides of the reagent rack 4 are fixedly connected with the inner wall of the reagent box body 1, and positioning jacks 11 are arranged at the corner positions of the top end of the reagent box body 1;

reagent jacks 9 are arranged at equal intervals in the reagent rack 4, and clamping mechanisms 10 are arranged in the reagent jacks 9;

a driving cavity 1001, a lever 1002, a push rod 1003, a tray 1004, a clamping jaw 1005, a driving rod 1006 and a supporting spring 1007 are sequentially arranged inside the clamping mechanism 10, the driving cavity 1001 is arranged inside the reagent rack 4 on one side of a reagent jack 9, the lever 1002 is installed at the bottom of the driving cavity 1001, the tray 1004 is arranged inside the reagent jack 9 above the lever 1002, the clamping jaw 1005 is movably connected to the top end of the tray 1004, the push rod 1003 is fixed to the bottom end of the tray 1004, the bottom end of the push rod 1003 is movably connected with one side of the top end of the lever 1002, the supporting spring 1007 is fixed to one side of the bottom end of the lever 1002, the bottom end of the supporting spring 1007 is fixedly connected with the inner wall of the bottom of the driving cavity 1001, the driving rod 1006 is movably connected to the top end of the lever 1002 at the position of the supporting spring 1007, and the top end of the driving rod 1006 extends to the upper side of the reagent rack 4;

pressing the driving rod 1006 to move downwards, driving the lever 1002 to be in an inclined state by the driving rod 1006, compressing the supporting spring 1007 by one end of the lever 1002, lifting the tray 1004 inside the reagent jack 9 by the other end of the lever 1002 through the push rod 1003, at the moment, moving the tray 1004 upwards, opening the clamping jaw 1005 at the top end of the tray 1004, then inserting the reagent tube into the reagent jack 9, loosening the driving rod 1006, and enabling the lever 1002 to recover to a horizontal state under the elastic reset action of the supporting spring 1007, at the moment, driving the clamping jaw 1005 to be retracted into the reagent jack 9 by the tray 1004, and under the limiting action of the reagent jack 9, folding the clamping jaw 1005 and clamping the reagent tube, so that the reagent tube is clamped and fixed conveniently, and the toppling of the reagent tube is reduced;

install temperature sensor 7 on the reagent box body 1 inner wall of reagent frame 4 top, this temperature sensor 7's model can be PT100, and temperature sensor 7's surface mounting has test probe 8, is convenient for detect the inside temperature of reagent box body 1 to prevent the deposit of the too high influence reagent of temperature.

The working principle is as follows: firstly, pressing a driving rod 1006 to move downwards, driving the lever 1002 to be in an inclined state by the driving rod 1006, compressing a supporting spring 1007 by one end of the lever 1002, lifting a tray 1004 inside a reagent jack 9 by the other end of the lever 1002 through a push rod 1003, at the moment, moving the tray 1004 upwards, opening a clamping jaw 1005 at the top end of the tray 1004, then inserting a reagent tube into the reagent jack 9, loosening the driving rod 1006, enabling the lever 1002 to be restored to a horizontal state under the elastic reset action of the supporting spring 1007, at the moment, driving the clamping jaw 1005 to be retracted into the reagent jack 9 by the tray 1004, folding the clamping jaw 1005 to clamp the reagent tube under the limiting action of the reagent jack 9, thereby clamping and fixing the reagent tube, reducing the toppling of the reagent tube, then, holding a handle 6 and covering a dustproof cover 5 at the top end of a reagent box body 1, the positioning inserted bar 13 at the bottom end of the dustproof cover 5 is clamped into the positioning insertion hole 11 at the top end of the reagent box body 1, the positioning inserted bar 13 is matched with the positioning insertion hole 11 to fix the dustproof cover 5, a cavity 12 in the dustproof cover 5 also provides enough space for storing a reagent tube, the reagent box body 1 is in a sealed state due to the arrangement of the dustproof cover 5, thereby reducing the entering of dust, finally, in the using process of the reagent box, the temperature sensor 7 detects the temperature in the reagent box body 1 through the detection probe 8, when the temperature in the reagent box body 1 exceeds the optimal temperature for storing the reagent tube, the refrigerating tube 15 in the refrigerating cavity 14 cools the interior of the reagent box body 1, and the temperature insulation sleeve 2 sleeved outside the reagent box body 1 separates the heat exchange inside and outside the reagent box body 1, thereby preventing the reagent from being influenced by the external environment and further reducing the deterioration of the reagent, the work of the kit for activating a squamous carcinoma-specific immune response was completed.

It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Claims

1. The utility model provides a kit for activating squamous carcinoma specific immunoreaction, includes reagent box body (1), separates temperature cover (2), reagent frame (4), fixture (10) and refrigeration pipe (15), its characterized in that: the reagent box comprises a reagent box body (1), a temperature insulation sleeve (2) is sleeved outside the reagent box body (1), a damping sponge cushion (3) is fixed at the bottom of the reagent box body (1), a reagent rack (4) is arranged inside the reagent box body (1) above the damping sponge cushion (3), outer walls of two sides of the reagent rack (4) are fixedly connected with the inner wall of the reagent box body (1), temperature sensors (7) are installed on the inner wall of the reagent box body (1) above the reagent rack (4), a detection probe (8) is installed on the surface of each temperature sensor (7), positioning jacks (11) are arranged at corner positions of the top end of the reagent box body (1), reagent jacks (9) with equal intervals are arranged inside the reagent rack (4), clamping mechanisms (10) are arranged inside the reagent jacks (9), a driving cavity (1001), a lever (1002), a push rod (1003), a tray (1004), clamping jaws (1005), a driving rod (1006) and a supporting spring (1007) are sequentially arranged inside the reagent rack (4) on one side of the reagent jack (9), the driving cavity (1001), a lever (1002) is arranged above the driving cavity (1002), and a reagent tray (1002) is provided with a reagent jack (1002), the reagent box comprises a tray (1004), a clamping jaw (1005) is movably connected to the top end of the tray (1004), a push rod (1003) is fixed to the bottom end of the tray (1004), the bottom end of the push rod (1003) is movably connected to one side of the top end of a lever (1002), a supporting spring (1007) is fixed to one side of the bottom end of the lever (1002), the bottom end of the supporting spring (1007) is fixedly connected to the inner wall of the bottom of a driving cavity (1001), a driving rod (1006) is movably connected to the top end of the lever (1002) at the position of the supporting spring (1007), the top end of the driving rod (1006) extends to the upper portion of a reagent frame (4), four equal-fixedly-connected supporting columns (18) at the bottom of a temperature insulation sleeve (2), four equal-connected non-slip mats (22) at the bottom of the supporting columns (18), a plurality of connecting columns (21) are fixedly arranged inside the temperature insulation sleeve (2), protective mats (17) are fixedly connected to the two sides of the temperature insulation sleeve (2), a refrigeration cavity (14) is arranged in the side wall of the bottom of the reagent box body (1), equidistant refrigeration tubes (15) are arranged inside the refrigeration tubes (14), a plurality of refrigeration tubes (5) are arranged inside the dustproof covers (12) arranged inside the dustproof cover (5), and a plurality of dustproof cover (12) is arranged inside the dustproof cover (12) arranged inside the dustproof cover (5) arranged inside the cavity (12), the top of shield (5) is provided with handle (6), the bottom of handle (6) and the top fixed connection of shield (5), handheld cover (16) of the top fixedly connected with of handle (6), a plurality of anti-skidding lines have been seted up on the surface of handheld cover (16), two equal fixedly connected with protection frame (19) in corner of shield (5), the corner position department of shield (5) bottom all is fixed with location inserted bar (13), and the bottom of location inserted bar (13) extends to the inside of location jack (11), the biggest diameter of location inserted bar (13) is less than the minimum diameter of location jack (11), and the maximum length of location inserted bar (13) is less than the minimum length of location jack (11).