CN113181981A - Digital microfluidic multiple detection system for autoimmune antibody - Google Patents
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
- B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
- G01N33/5304—Reaction vessels, e.g. agglutination plates
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/564—Immunoassay; Biospecific binding assay; Materials therefor for pre-existing immune complex or autoimmune disease, i.e. systemic lupus erythematosus, rheumatoid arthritis, multiple sclerosis, rheumatoid factors or complement components C1-C9
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
Abstract
The invention provides a digital microfluidic autoimmune antibody detection system. The method comprises the steps that a single chip comprises a sample for full-flow autoimmune antibody detection, all operations related to the experimental flow, including sample quantitative distribution, reagent quantitative distribution, solution transfer, incubation reaction, antigen cleaning and the like, are automatically completed by a digital microfluidic chip, finally, the detection result is judged through fluorescence detection, and a user can realize full-flow multiple autoimmune antibody detection only by loading a sample on the chip.
Description
Technical Field
The invention belongs to the technical field of digital microfluidic control, and relates to a digital microfluidic multiple detection system for an autoimmune antibody.
Background
Autoimmune antibody detection is one of important detection means of autoimmune diseases, the current domestic self-immune detection technology is still in the starting stage, and the detection process mainly depends on manual operation or a large workstation based on a mechanical arm. The domestic self-immunity detection market is currently mainly occupied by international companies, such as the Saimei fly PhadiaTMThe 250 immunoassay analyzer is a workstation based on a mechanical arm, is suitable for medium-sized laboratories, and can provide 400 to 2000 detection knots per weekAnd (5) fruit.
Because the self-exempting detection process is relatively complicated and consumes a long time and has low detection efficiency, the self-exempting detection is easy to lose due to misoperation in the manual operation process. The large workstation based on the mechanical arm is large in size and high in cost, and is only suitable for large enterprises, central laboratories, hospitals and other large-scale mechanisms. At present, a low-cost miniaturized automatic self-free detection system is lacked in the market. Phadia of Saimei flyTMThe 250 immunoassay analyzer is high in cost, large in size and limited in use scene, and is difficult to meet the huge self-free detection requirement.
Therefore, there is a need to provide a new fully automatic digital microfluidic autoimmune antibody multiplex detection system with high integration, low cost and high reliability.
Disclosure of Invention
In response to the deficiencies and practical needs of the prior art, the present invention provides a digital microfluidic autoimmune antibody detection system. The method comprises the steps that a single chip comprises a sample for full-flow autoimmune antibody detection, all operations related to the experimental flow, including sample quantitative distribution, reagent quantitative distribution, solution transfer, incubation reaction, antigen cleaning and the like, are automatically completed by a digital microfluidic chip, finally, the detection result is judged through fluorescence detection, and a user can realize full-flow multiple autoimmune antibody detection only by loading a sample on the chip.
In order to achieve the purpose, the invention adopts the following technical scheme:
in a first aspect, the present invention provides a digital microfluidic chip for detecting an autoimmune antibody, wherein a sample and a reagent loading position are shown in fig. 1 to 3, and the chip mainly comprises an upper cover 1, a fixed antigen 2, a conductive layer 3, a hydrophobic layer 4, a dielectric layer 5, a substrate 6, an electrode 7, a reagent and sample distribution region 8, a waste liquid region 9, a sample solution 10, a cleaning solution 11, and a secondary antibody solution 12 containing a fluorescent label;
preferably, the sample is whole blood, serum, plasma, or the like;
preferably, the antigen 2 is fixed by injecting the antigen into the lower surface of the upper cover 1 according to a predetermined volume and air-drying, wherein the lower surface is the surface facing the electrode;
preferably, the antigen is injected to the lower surface of the upper cover 1 by a robot arm;
preferably, the fixed antigen 2 can be n, n is a positive integer no less than 1, for example, n can be 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 30, 40, 50, 60, etc.;
preferably, the chip may contain a plurality of differently sized electrodes 7;
preferably, the chip can contain m secondary antibody solutions containing fluorescent labels, wherein m is a positive integer greater than or equal to 1, for example, m can be 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 30, 40, 50, etc.;
preferably, the reagent can be loaded in a manner of injection by a pipette, loading by an independent reagent pack or pre-embedding in a chip.
In a second aspect, the present invention provides a digital microfluidic multiple detection system for autoimmune antibodies, comprising the digital microfluidic detection chip for autoimmune antibodies of the first aspect;
preferably, the digital microfluidic multiple detection system for the autoimmune antibody comprises a fluorescence detection module, and can realize qualitative or quantitative detection;
preferably, the digital microfluidic autoimmune antibody multiplex detection system comprises an electrode design or cascade, and can realize higher detection flux.
In a third aspect, the present invention provides a method for performing digital microfluidic multiple detection of autoimmune antibodies using the chip of the first aspect or the system of the second aspect, the method comprising:
(1) loading a chip on the detection equipment, filling the chip with biocompatible medium oil, and loading a sample and a reagent at a corresponding inlet according to a specified volume;
(2) quantitatively distributing the sample 10 into n sample droplets 13, respectively transferring the sample droplets 13 to the positions of n fixed antigens, carrying out incubation reaction, and transferring the sample droplets 13 to a waste liquid area 9 for discarding after the incubation reaction is finished;
(3) distributing n cleaning solution droplets 14 from the cleaning solution 11, cleaning the immobilized antigen, and transferring the cleaning solution droplets 14 to the waste solution area 9 for discarding after the cleaning is finished;
(4) quantitatively distributing the secondary antibody solution containing the fluorescent marker into n sample liquid drops 15, respectively transferring the sample liquid drops 15 to the positions of n fixed antigens, carrying out incubation reaction, and transferring the sample liquid drops 15 to a waste liquid area 9 for discarding after the incubation reaction is finished;
(5) distributing n cleaning solution droplets 14 from the cleaning solution 11, cleaning the immobilized antigen, and transferring the cleaning solution droplets 14 to the waste solution area 9 for discarding after the cleaning is finished;
(6) performing fluorescence detection on the positions of the n fixed antigens, and performing qualitative or quantitative detection on the content of the antibody in the sample;
preferably, the n immobilized antigens are arranged in m columns, the secondary antibody solution containing the fluorescent label is m, and the first secondary antibody solution containing the fluorescent label, the second secondary antibody solution containing the fluorescent label … … and the m secondary antibody solution containing the fluorescent label are respectively reacted with the first column of immobilized antigens, the second example of immobilized antigens … … and the m column of immobilized antigens;
wherein n is a positive integer of 1 or more, for example, n may be 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 30, 40, 50, 60, etc.; m is a positive integer of 1 or more, for example, m may be 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 30, 40, 50, or the like;
preferably, the fluorescence detection is to realize qualitative or quantitative detection of the antibody in the sample by adopting a fluorescence detection module;
preferably, the temperature of the incubation reaction is 4-45 ℃, preferably 37 ℃; the incubation reaction time is 1-30min, preferably 3-5 min.
Compared with the prior art, the invention has the following beneficial effects:
(1) the digital microfluidic chip provided by the invention replaces manual operation, so that the self-exempting detection efficiency and accuracy are greatly improved, the integration level is high, the detection method is short in time consumption, the cost of the digital microfluidic chip is low, and the reliability is high;
(2) the chip type totally-enclosed detection environment has no pollution risk and high flexibility, is suitable for the self-exempting detection of various samples, and has small equipment volume and low equipment cost far lower than a workstation based on a mechanical arm;
(3) the digital microfluidic multiple detection system for the autoimmune antibody has high expansibility, can realize higher detection flux through electrode design or cascade, has multiple detection capability, and can realize customized experimental processes.
Drawings
Fig. 1 is an antigen distribution diagram of an upper cover of a digital microfluidic autoimmune antibody detection chip, which mainly comprises an upper cover 1 and a plurality of fixed antigens 2. The antigen is injected to the lower surface (i.e. the surface facing the electrode) of the upper cover 1 according to a predetermined volume, and the antigen fixation is realized by air drying, and multiple antigens can be fixed on one upper cover to realize multiple detections.
Fig. 2 is a cross-sectional view of a chip comprising a cover 1, immobilized antigens 2, a conductive layer 3, a hydrophobic layer 4, a dielectric layer 5, a substrate 6 and electrodes 7. .
Fig. 3 is a design diagram of a digital microfluidic chip for detecting autoimmune antibodies, which comprises a plurality of fixed antigens 2 on the lower surface of an upper cover, a plurality of electrodes 7 with different shapes and sizes, a reagent and sample distribution area 8, a waste liquid area 9, a sample solution 10, a cleaning solution 11, and a secondary antibody solution 12 containing a fluorescent label.
FIG. 4 is a schematic diagram of the incubation reaction of a sample solution with an immobilized antigen.
FIG. 5 is a schematic diagram of the first washing of the washing solution to immobilize the antigen.
FIG. 6 is a schematic diagram of the incubation reaction of a secondary antibody with an immobilized antigen.
FIG. 7 is a schematic diagram of the second washing with the washing solution to immobilize the antigen.
FIG. 8 is a schematic view of fluorescence detection.
Fig. 9 is a design diagram of a digital microfluidic chip for detecting an autoimmune antibody capable of simultaneously performing two types of secondary antibodies, which includes a plurality of immobilized antigens 2 on the lower surface of an upper cover, a reagent and sample distribution region 8, a waste liquid region 9, a sample solution 10, a cleaning solution 11, a first type of secondary antibody solution 12 containing a fluorescent label, and a second type of secondary antibody solution 16 containing a fluorescent label.
Detailed Description
To further illustrate the technical means adopted by the present invention and the effects thereof, the present invention is further described below with reference to the embodiments and the accompanying drawings. It is to be understood that the specific embodiments described herein are merely illustrative of the invention and are not limiting of the invention.
The examples do not show the specific techniques or conditions, according to the technical or conditions described in the literature in the field, or according to the product specifications. The reagents or apparatus used are conventional products commercially available from normal sources, not indicated by the manufacturer.
Example 1: self-immune detection of 1 sample 8 antigens and 1 second antibody
(1) Loading the chip on the detection device and filling the chip with biocompatible medium oil, loading the sample and reagent at the corresponding inlet according to fig. 3 according to the specified volume (in this embodiment, 20 microliters of sample is required for a single reaction, and at least 160 microliters of sample needs to be injected into the chip);
(2) as shown in fig. 4, the sample 10 is quantitatively distributed into 8 sample droplets 13 according to the direction of the arrow in the figure and is transferred to the positions of 8 immobilized antigens respectively, an incubation reaction is performed for 3 minutes, the reaction temperature is usually 37 ℃, and the sample droplets 13 are transferred to the waste liquid area 9 and discarded after the incubation reaction is completed;
(3) as shown in fig. 5, 8 drops of the washing liquid 14 are dispensed from the washing liquid 11 in the direction of the arrow in the figure, the immobilized antigen is washed, and after the washing is completed, the washing liquid drops 14 are transferred to the waste liquid area 9 and discarded;
(4) as shown in fig. 6, quantitatively distributing the secondary antibody solution containing the fluorescent label into 8 sample droplets 15 in the direction of the arrow in the figure, transferring the sample droplets 15 to the positions of 8 immobilized antigens respectively, performing an incubation reaction for 3 minutes, wherein the reaction temperature is usually 37 ℃, and transferring the sample droplets 15 to a waste liquid area 9 for discarding after the incubation reaction is completed;
(5) as shown in fig. 7, 8 drops of the washing liquid 14 are dispensed from the washing liquid 11 in the direction of the arrow in the figure, the immobilized antigen is washed, and after the washing is completed, the washing liquid drops 14 are transferred to the waste liquid area 9 and discarded;
(6) as shown in FIG. 8, the fluorescence detection was performed for the positions of 8 immobilized antigens, and the qualitative or quantitative detection of the antibodies in the sample was performed.
Example 2: self-immune detection of 16 antigens and 2 secondary antibodies in 1 sample
Fig. 9 shows a design diagram of a digital microfluidic chip for detecting an autoimmune antibody capable of simultaneously performing two secondary antibodies, which comprises a plurality of immobilized antigens 2 on the lower surface of an upper cover, a reagent and sample distribution region 8, a waste liquid region 9, a sample solution 10, a cleaning solution 11, a first secondary antibody solution 12 containing a fluorescent label, and a second secondary antibody solution 16 containing a fluorescent label.
(1) Loading the chip on the detection device and filling the chip with biocompatible medium oil, loading the sample and reagent at the corresponding inlet according to fig. 9 according to the specified volume (in this embodiment, 20 microliters of sample is required for a single reaction, and at least 320 microliters of sample needs to be injected into the chip);
(2) in FIG. 9, two rows of immobilized antigens 2 are included, and a first secondary antibody solution 12 containing a fluorescent label and a second secondary antibody solution 16 containing a fluorescent label are reacted with each row, respectively, and the basic operation flow is the same as that of step 2 to step 6 in example 1.
The digital microfluidic autoimmune antibody multiple detection system can realize higher detection flux through electrode design or cascade.
In conclusion, the digital microfluidic chip provided by the invention replaces manual operation, so that the self-exempting detection efficiency and accuracy are greatly improved, the integration level is high, the detection method is short in time consumption, and the digital microfluidic chip is low in cost and high in reliability; the chip type totally-enclosed detection environment has no pollution risk and high flexibility, is suitable for the self-free detection of various samples, and has small equipment volume and low equipment cost far lower than a workstation based on a mechanical arm; the digital microfluidic multiple detection system for the autoimmune antibody has high expansibility, can realize higher detection flux through electrode design or cascade, has multiple detection capability, and can realize customized experimental processes.
The applicant states that the present invention is illustrated in detail by the above examples, but the present invention is not limited to the above detailed methods, i.e. it is not meant that the present invention must rely on the above detailed methods for its implementation. It should be understood by those skilled in the art that any modification of the present invention, equivalent substitutions of the raw materials of the product of the present invention, addition of auxiliary components, selection of specific modes, etc., are within the scope and disclosure of the present invention.
Claims (8)
1. A digital microfluidic autoimmune antibody detection chip comprises an upper cover (1), a fixed antigen (2), a conducting layer (3), a hydrophobic layer (4), a dielectric layer (5), a substrate (6), an electrode (7), a reagent and sample distribution area (8), a waste liquid area (9), a sample solution (10), a cleaning solution (11) and a secondary antibody solution (12) containing a fluorescent label;
the fixed antigen (2) is prepared by injecting antigen into the lower surface of the upper cover 1 according to a predetermined volume, and air-drying to fix the antigen, wherein the lower surface is the surface facing the electrode.
2. The digital microfluidic autoimmune antibody detection chip according to claim 1, wherein the number of the immobilized antigens (2) is n, and n is a positive integer not less than 1;
preferably, the chip comprises m secondary antibody solutions containing fluorescent labels, wherein m is a positive integer more than or equal to 1.
3. The digital microfluidic autoimmune antibody detection chip according to claim 2, wherein the chip is loaded on the detection device and filled with biocompatible medium oil;
preferably, the sample is whole blood, serum, plasma;
preferably, the antigen is injected to the lower surface of the upper cover (1) by a mechanical arm;
preferably, the sample and/or the reagent can be loaded in a manner of injection by a pipette, loading by an independent reagent pack or pre-embedding the reagent in the chip;
preferably, the chip may contain a plurality of differently sized electrodes (7).
4. A digital microfluidic autoimmune antibody multiplex detection system, characterized in that the system comprises the digital microfluidic autoimmune antibody detection chip of any one of claims 1 to 3.
5. The digital microfluidic autoimmune antibody multiplex detection system according to claim 4, characterized in that the system further comprises a fluorescence detection module to enable qualitative or quantitative detection;
preferably, the system further comprises an electrode design or a plurality of cascades of the chips to achieve a higher detection flux.
6. A method for performing digital microfluidic multiple detection of autoimmune antibodies using a chip according to any of claims 1 to 3 or a system according to any of claims 4 or 5, comprising:
(1) loading a chip on the detection equipment, filling the chip with biocompatible medium oil, and loading a sample and a reagent at a corresponding inlet according to a specified volume;
(2) quantitatively distributing the sample (10) into n sample liquid drops (13) and respectively transferring the sample liquid drops to the positions of n fixed antigens for incubation reaction, and transferring the sample liquid drops (13) to a waste liquid area (9) for discarding after the incubation reaction is finished;
(3) distributing n drops of cleaning solution droplets (14) from the cleaning solution (11), cleaning the immobilized antigen, and transferring the cleaning solution droplets (14) to a waste solution area (9) for discarding after the cleaning is finished;
(4) quantitatively distributing the secondary antibody solution containing the fluorescent marker into n sample liquid drops (15) and respectively transferring the sample liquid drops to the positions of n fixed antigens for incubation reaction, and transferring the sample liquid drops (15) to a waste liquid area (9) for discarding after the incubation reaction is finished;
(5) distributing n drops of cleaning solution droplets (14) from the cleaning solution (11), cleaning the immobilized antigen, and transferring the cleaning solution droplets (14) to a waste solution area (9) for discarding after the cleaning is finished;
(6) detecting the positions of the n fixed antigens, and performing qualitative or quantitative detection on the content of the antibody in the sample;
the positions of the n fixed antigens are arranged in m columns, the secondary antibody solution containing the fluorescent marker is m, and the first secondary antibody solution containing the fluorescent marker, the second secondary antibody solution containing the fluorescent marker … … and the mth secondary antibody solution containing the fluorescent marker respectively react with the first column of fixed antigens, the second example of fixed antigens … … and the mth column of fixed antigens;
wherein n is a positive integer not less than 1; and m is a positive integer not less than 1.
7. The digital microfluidic autoimmune antibody multiplex detection method according to claim 6, wherein the detection in step (6) is qualitative or quantitative detection of the antibody in the sample by using a fluorescence detection module.
8. The method for the multiplex detection of digital microfluidic autoimmune antibodies according to claim 6, characterized in that the temperature of the incubation reaction is 4-45 ℃, preferably 37 ℃;
preferably, the incubation reaction time is 1-30min, preferably 3-5 min.
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| CN202110460449.6A CN113181981A (en) | 2021-04-27 | 2021-04-27 | Digital microfluidic multiple detection system for autoimmune antibody |
| CN202210829811.7A CN115193495A (en) | 2021-04-27 | 2021-04-27 | Digital microfluidic multiple detection system for autoimmune antibody |
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| CN202110460449.6A CN113181981A (en) | 2021-04-27 | 2021-04-27 | Digital microfluidic multiple detection system for autoimmune antibody |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2771864Y (en) * | 2005-03-22 | 2006-04-12 | 山东省医药生物技术研究中心 | Protein chip for detecting blood cerebrospinal fluid pathogenic antibody |
| CN104360060A (en) * | 2014-11-14 | 2015-02-18 | 国家纳米科学中心 | Detection method for specific antibodies IgM of mycoplasma pneumonia and influenza viruses based on micro-fluidic chip |
| CN109557150A (en) * | 2019-01-14 | 2019-04-02 | 大连大学 | Digital microcurrent-controlled chip and pathogen immunologic detection method based on it |
| CN111398236A (en) * | 2020-05-07 | 2020-07-10 | 成都量蚁科技有限公司 | Micro-fluidic chip detection system, application and antigen-antibody immunodetection method |
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2021
- 2021-04-27 CN CN202210829811.7A patent/CN115193495A/en active Pending
- 2021-04-27 CN CN202110460449.6A patent/CN113181981A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2771864Y (en) * | 2005-03-22 | 2006-04-12 | 山东省医药生物技术研究中心 | Protein chip for detecting blood cerebrospinal fluid pathogenic antibody |
| CN104360060A (en) * | 2014-11-14 | 2015-02-18 | 国家纳米科学中心 | Detection method for specific antibodies IgM of mycoplasma pneumonia and influenza viruses based on micro-fluidic chip |
| CN109557150A (en) * | 2019-01-14 | 2019-04-02 | 大连大学 | Digital microcurrent-controlled chip and pathogen immunologic detection method based on it |
| CN111398236A (en) * | 2020-05-07 | 2020-07-10 | 成都量蚁科技有限公司 | Micro-fluidic chip detection system, application and antigen-antibody immunodetection method |
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Application publication date: 20210730 |