CN113180873A - Fixing device and method for application of fish brain control technology - Google Patents
Fixing device and method for application of fish brain control technology Download PDFInfo
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- CN113180873A CN113180873A CN202110402245.7A CN202110402245A CN113180873A CN 113180873 A CN113180873 A CN 113180873A CN 202110402245 A CN202110402245 A CN 202110402245A CN 113180873 A CN113180873 A CN 113180873A
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- 241000251468 Actinopterygii Species 0.000 title claims abstract description 125
- 210000004556 brain Anatomy 0.000 title claims abstract description 51
- 238000000034 method Methods 0.000 title claims abstract description 12
- 238000000429 assembly Methods 0.000 claims abstract description 21
- 230000000712 assembly Effects 0.000 claims abstract description 21
- 238000002513 implantation Methods 0.000 claims abstract description 16
- 210000003625 skull Anatomy 0.000 claims description 15
- 241000252233 Cyprinus carpio Species 0.000 claims description 14
- 210000005013 brain tissue Anatomy 0.000 claims description 8
- 238000002474 experimental method Methods 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 229920000742 Cotton Polymers 0.000 claims description 7
- RRAFCDWBNXTKKO-UHFFFAOYSA-N eugenol Chemical compound COC1=CC(CC=C)=CC=C1O RRAFCDWBNXTKKO-UHFFFAOYSA-N 0.000 claims description 6
- 230000000638 stimulation Effects 0.000 claims description 6
- 238000007428 craniotomy Methods 0.000 claims description 4
- 206010002091 Anaesthesia Diseases 0.000 claims description 3
- NPBVQXIMTZKSBA-UHFFFAOYSA-N Chavibetol Natural products COC1=CC=C(CC=C)C=C1O NPBVQXIMTZKSBA-UHFFFAOYSA-N 0.000 claims description 3
- 239000005770 Eugenol Substances 0.000 claims description 3
- UVMRYBDEERADNV-UHFFFAOYSA-N Pseudoeugenol Natural products COC1=CC(C(C)=C)=CC=C1O UVMRYBDEERADNV-UHFFFAOYSA-N 0.000 claims description 3
- 230000037005 anaesthesia Effects 0.000 claims description 3
- 210000000988 bone and bone Anatomy 0.000 claims description 3
- 239000003479 dental cement Substances 0.000 claims description 3
- 229960002217 eugenol Drugs 0.000 claims description 3
- 238000003801 milling Methods 0.000 claims description 3
- 230000006378 damage Effects 0.000 abstract description 5
- 208000027418 Wounds and injury Diseases 0.000 abstract description 4
- 208000014674 injury Diseases 0.000 abstract description 4
- 210000004369 blood Anatomy 0.000 abstract description 2
- 239000008280 blood Substances 0.000 abstract description 2
- 230000010412 perfusion Effects 0.000 abstract description 2
- 210000003484 anatomy Anatomy 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 4
- 230000001537 neural effect Effects 0.000 description 2
- 230000000149 penetrating effect Effects 0.000 description 2
- 241000252210 Cyprinidae Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010241 blood sampling Methods 0.000 description 1
- 230000036461 convulsion Effects 0.000 description 1
- 238000002224 dissection Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D3/00—Appliances for supporting or fettering animals for operative purposes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D7/00—Devices or methods for introducing solid, liquid, or gaseous remedies or other materials into or onto the bodies of animals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
- A61N1/00—Electrotherapy; Circuits therefor
- A61N1/02—Details
- A61N1/04—Electrodes
- A61N1/05—Electrodes for implantation or insertion into the body, e.g. heart electrode
- A61N1/0526—Head electrodes
- A61N1/0529—Electrodes for brain stimulation
- A61N1/0539—Anchoring of brain electrode systems, e.g. within burr hole
Abstract
The invention relates to a fixing device and a method thereof for fish brain control technology application, wherein the device comprises a box body, a fish head fixing assembly, a fish body clamping assembly, a clamping height adjusting assembly, a clamping tightness adjusting assembly and an electrode clamping assembly; the fish head fixing component is arranged at the front side inside the box body; the fish body clamping assemblies are symmetrically arranged on the left side and the right side of the interior of the box body; the clamping height adjusting assemblies are symmetrically arranged on the left side and the right side of the box body and correspond to the fish body clamping assemblies one by one; the clamping tightness adjusting assembly is respectively connected with the fish body clamping assembly and the clamping height adjusting assembly and is arranged on the left side and the right side of the outer part of the box body; the electrode clamping assembly is arranged on the outer side of the front end of the box body. The invention can solve the problems of brain fixation during brain electrode implantation operation, fretting injury generated during brain electrode implantation process and the like, and can be effectively applied to the aspects of fish operation, anatomy, blood collection, heart perfusion, brain science, biological control and the like.
Description
Technical Field
The invention relates to the technical field of biological control, in particular to a fixing device and a method for application of a fish brain control technology.
Background
The control of various animal robots is realized internationally by applying a brain control technology, and is mainly realized by applying an analog electrophysiological signal through brain electrodes implanted in a brain moving area. However, in the process of implanting the electrode into the brain tissue of the fish, if the head of the fish is not firmly fixed, the fish itself has twitch reaction and the electrode is slightly moved when being fixed, the implantation of the brain electrode is inaccurate and the brain tissue is locally damaged.
Yangylin discloses a research fin motion laboratory bench (CN204724203U), including fixation clamp, fixed station, fixed bolster, spliced pole, fixation nut, fish mouth fixing device, the device is only the fixed part fish body, unset fin for observe each fin motion, the fish head inserts the fish mouth dead lever on the fish mouth fixing device and realizes fixing with the fish mouth, fin motion can directly be observed to such fixed mode, but can not carry out upper and lower, left and right, preceding, all-round fixed after to the fish head, can not solve the problem that the brain electrode produced the fretting damage when implanting. And bin, etc. disclose a live fish experimental apparatus (CN111265327A), including fixed station, fluid bath, fixation clamp, this device can firmly fix the fish body, mainly used for blood sampling and medicine injection of live fish, but the fixation clamp that this device was used for fixed fish head is the same with fixed fish body fixation clamp, is unfavorable for fish head operation.
Although there is a fixing device for fish in the prior art, it is mainly for fixing the fish body, and it can not meet the brain fixing requirement when implanting brain electrode operation, and the application range is relatively limited, so the prior art means has a space for further improvement and perfection.
Disclosure of Invention
In view of the above problems, an object of the present invention is to provide a fixing device and a method thereof for fish brain control technology application, which can solve the problems of brain fixation during brain electrode implantation operation and micro-motion injury during brain electrode implantation, and can be applied to fish operations, dissection, blood collection, heart perfusion, brain science, biological control, and the like.
The technical scheme adopted by the invention is as follows:
the invention provides a fixing device for fish brain control technology application, which comprises a box body, a fish head fixing assembly, a fish body clamping assembly, a clamping height adjusting assembly, a clamping tightness adjusting assembly and an electrode clamping assembly, wherein the fish head fixing assembly is arranged on the box body; the fish head fixing assembly is connected to the front side of the box body in a sliding manner, the fish body clamping assemblies are symmetrically arranged on the left side and the right side of the interior of the box body, the clamping height adjusting assemblies are respectively arranged on the outer sides of the middle parts of the fish body clamping assemblies, the clamping height adjusting assemblies are connected with the side walls of the box body in a sliding manner, and the clamping tightness adjusting assemblies are arranged at the outer ends of the clamping height adjusting assemblies; the electrode clamping assembly is arranged on the outer side of the front end of the box body;
the box body comprises a box frame, an inner sliding chute, a tail chute, a sliding chute I, an outer sliding chute, a sliding chute II, a fixed seat, a fixed hole, a fixed screw hole, a sliding chute III, an arc-shaped sliding chute and a water leakage hole; the box frame comprises box frames, wherein the box frames are arranged on the box frames, the inner sliding grooves are symmetrically and uniformly distributed on two sides in the long edge of each box frame, two through grooves, namely a sliding groove I and a sliding groove II, are arranged in each inner sliding groove, an outer sliding groove is arranged on the outer side of each inner sliding groove corresponding to each box frame, a tail groove is arranged at the middle upper part of the short edge on one side of each box frame, an arc-shaped sliding groove is arranged at the bottom of the short edge on the other side of each box frame, protruding parts are symmetrically arranged on the upper parts of;
furthermore, the fish head fixing assembly comprises a support rod, a support table, a screw, a sliding rod, a nut, a wing nut and an arc-shaped fixing arm; the support rod penetrates through the fixed seat, the support rod is arranged to be a step cylinder, one end with a small diameter is connected with the support table, one end with a large diameter can slide in the fixed hole to adjust the position, the screw is arranged in a fixed screw hole in the side face of the fixed seat, the arc-shaped fixed arms are symmetrically arranged on the left side and the right side of the front end of the box frame and used for pressing the fish head, the radian of the arc-shaped fixed arms is consistent with that of a fish skull, the arc-shaped fixed arms are in threaded connection with the inner ends of the slide rods through nuts, the slide rods are arranged in slide grooves III on the two sides of the box frame, and the wing-shaped nuts are arranged at the outer ends of the joints of the slide rods and the slide grooves III; the inner side surfaces of the support platform and the arc-shaped fixed arm are both provided with cotton layers.
Further, the fish body clamping assembly comprises a clamping plate and a connecting seat, wherein a cotton layer is attached to the inner side of the clamping plate; the connecting seat is fixed on the lower part of the outer side of the clamping plate, and a threaded hole is formed in the outer end face of the connecting seat.
Further, the clamping height adjusting assembly comprises a sliding block, a bolt and a fixing groove; the slider is installed in interior spout department, be provided with a through-hole and a screw hole on the slider, the outer spout department in the box body is installed to the slide bar fixed slot, bolt one end is connected in the threaded hole of slider after passing slide bar fixed slot and box body in proper order from the box body outside.
Furthermore, the middle part of the fixing groove is provided with a sliding groove, the inner end of the fixing groove is provided with a baffle corresponding to the sliding block, the baffle is installed at the outer sliding groove, and the baffle is provided with a bolt hole and a sliding hole.
Further, the clamping tightness adjusting assembly comprises an L-shaped sliding rod and a fixing nut; the L-shaped sliding rod is installed on the fixing groove, one end of the L-shaped sliding rod penetrates through the sliding hole in the baffle and the through hole in the sliding block and then is fixedly connected with the connecting seat, the other end of the L-shaped sliding rod is slidably connected with the sliding groove in the middle of the fixing groove, and the outer side of the connecting position is locked through the fixing nut.
Further, the electrode clamping assembly comprises a bolt, a wing nut, a rotating rod, a clamping bottom plate, a clamping plate, a spring and a special-shaped U-shaped clamp; the special-shaped U-shaped clamp is characterized in that the bolt sequentially penetrates through the arc-shaped sliding groove and the bottom of the rotating rod, the bolt is fixed to the bottom of the rotating rod through a wing nut, the top of the rotating rod is connected with the special-shaped U-shaped clamp in a sliding mode, the opening end of the special-shaped U-shaped clamp is locked through the wing nut, the special-shaped U-shaped clamp can rotate around the rotating rod, a rod with threads is arranged on one side of the special-shaped U-shaped clamp, the rod penetrates through the clamping bottom plate and is fixed through the nut, the clamping bottom plate can rotate around the rod of the special-shaped U-shaped clamp, the lower portion of the clamping bottom plate.
A fixing method for application of a fish brain control technology comprises the following steps:
step S1: selecting a healthy adult carp, and placing the carp in a eugenol solution for medicated bath anesthesia;
step S2: placing the anesthetized carp into the box body, placing the fish head on a supporting table of the fish head fixing component, placing the fish body into the fish body clamping component, and adjusting the heights of the supporting table and the clamping height adjusting component to reach proper positions so that the skull surface of the carp is parallel to the horizontal plane; screwing and fixing the screw of the fish head fixing component and the bolt of the clamping height adjusting component; attaching the arc-shaped fixing arm to the skull of the fish head, and screwing down a nut and a wing nut of the fish head fixing assembly for fixing; according to the size of the fish body, a fixing nut for clamping the elastic component is screwed up and fixed to clamp and fix the fish body;
step S3: craniotomy is performed by using a bone milling drill to expose brain tissues;
step S4: placing the brain electrode between the clamping bottom plate and the clamping plate, screwing the wing nut on the clamping plate, adjusting a proper brain electrode implantation angle according to the implantation site of brain tissue, screwing the wing nut on the lower part of the rotating rod, slowly pushing the special-shaped U-shaped clamp downwards after fixation, and screwing the wing nut on the special-shaped U-shaped clamp until the brain electrode is implanted completely;
step S5: smearing dental cement between the skull and the brain electrode to fix the brain electrode;
step S6: after the fixing is finished, all the fixing and clamping components are loosened, and the carp can be subjected to an off-water electrical stimulation experiment and an underwater electrical stimulation control experiment.
Compared with the prior art, the invention has the following beneficial effects:
the fish fixing device can be used for fixing fishes in an up-down, left-right, front-back and all-around manner, is convenient for experimental operation, and improves the safety and the stability; the surface of the skull of the fish head can be adjusted to be parallel to the horizontal plane, so that the brain electrode can be conveniently implanted, and the implantation precision is improved; but also can reduce the fretting injury generated in the brain electrode implantation process; meanwhile, the utility model can prevent the stains from splashing and has higher practical application value.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic top view of the overall structure of the present invention;
FIG. 3 is a right side view of the overall structure of the present invention;
FIG. 4 is a schematic structural view of the cartridge body;
FIG. 5 is a schematic cross-sectional view of the cartridge;
FIG. 6 is a schematic structural view of the fish head fixing assembly;
FIG. 7 is a schematic view of the fish body clamp assembly;
FIG. 8 is a schematic view of the clamp height adjustment assembly;
fig. 9 is a structural view of a slide bar fixing groove;
FIG. 10 is a schematic view of the clamping slack adjuster assembly;
FIG. 11 is a schematic structural view of an electrode clamping assembly;
fig. 12 is a front view of the electrode clamping assembly.
Wherein, the reference numbers: a box body 1; a box frame 1-1; an inner chute 1-2; 1-3 of a tail groove; 1-4 of a chute; 1-5 of an outer chute; a chute II 1-6; 1-7 of a chute III; 1-8 arc chutes; 1-9 of a fixing hole; fixing screw holes 1-10; fixing seats 1-11; 1-12 water leakage holes; a fish head fixing component 2; 2-1 of a support rod; a support table 2-2; 2-3 parts of a screw; 2-4 of a sliding rod; 2-5 of a nut; 2-6 parts of wing nut; an arc-shaped fixed arm 2-7; a fish body clamping component 3; a connecting seat 3-1; a splint 3-2 with a cotton layer on the inner side; a clamping height adjustment assembly 4; 4-1 of a slide block; 4-2 of a fixed groove; 4-2-1 of a slide hole; a chute 4-2-2; bolt holes 4-2-3; 4-3 of bolts; clamping the tightness adjusting assembly 5; an L-shaped sliding rod 5-1; 5-2 of a fixed nut; an electrode clamping assembly 6; 6-1 of a bolt; wing nuts 6-2; rotating the rod 6-3; 6-4 of a special-shaped U-shaped clamp; 6-5 of a clamping bottom plate; 6-6 of a rod; 6-7 of a clamping plate; and 6-8 of springs.
Detailed Description
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
It should be noted that in the description of the present invention, the terms "upper", "lower", "top", "bottom", "one side", "the other side", "left", "right", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of describing the present invention and simplifying the description, but do not mean that a device or an element must have a specific orientation, be configured and operated in a specific orientation.
Referring to fig. 1 to 4, a specific structure of an embodiment of a fixing device applied to a fish brain control technology is shown. The device comprises a device box body 1, a fish head fixing component 2, a fish body clamping component 3, a clamping height adjusting component 4, a clamping tightness adjusting component 5 and an electrode clamping component 6; the fish head fixing assembly 2 is connected to the front side of the box body 1 in a sliding mode, the fish body clamping assemblies 3 are symmetrically arranged on the left side and the right side of the interior of the box body, the clamping height adjusting assemblies 4 are respectively arranged on the outer sides of the middle portions of the fish body clamping assemblies 3, the clamping height adjusting assemblies 4 are connected with the side walls of the box body 1 in a sliding mode, and the clamping tightness adjusting assemblies 5 are arranged at the outer ends of the clamping height adjusting assemblies 4; the electrode clamping assembly 6 is arranged on the outer side of the front end of the box body 1; in this embodiment, the box body 1 is a rectangular parallelepiped structure; the fish head fixing component 2 can adjust the height of the fish head and then fix the fish head in all directions according to different types and sizes of fish; the fish body clamping assembly 3 is used for clamping a fish body; the clamping height adjusting component 4 can adjust the height of the fish body clamping component 3 and fix the fish body clamping component; the clamping tightness adjusting component 5 can adjust the tightness of the fish body clamping component 3 and fix the fish body clamping component; the fish head fixing component 2, the fish body clamping component 3, the clamping height adjusting component 4 and the clamping tightness adjusting component 5 are used in a matched mode, the surface of a skull of a fish head can be adjusted to be parallel to a horizontal plane, implantation of electrodes is facilitated, and experiment precision is improved; the electrode clamping component 6 can solve the problem of fretting injury caused by brain electrode implantation, and can also be used for clamping neural prostheses and the like.
The box body 1 comprises a box frame 1-1, an inner chute 1-2, a tail chute 1-3, a chute I1-4, an outer chute 1-5, a chute II1-6, a chute III 1-7, an arc chute 1-8, a fixing seat hole 1-9, a fixing screw hole 1-10, a fixing seat 1-11 and a water leakage hole 1-12; the box frame 1-1 can effectively prevent stains from flying in a mess way in the craniotomy process; in the embodiment, the inner chutes 1-2 are symmetrically and uniformly distributed on two sides in the long side of the box frame 1-1, each side is three, two through grooves are arranged in each inner chute 1-2, namely, a chute I1-4 and a chute II 1-5, each inner chute 1-2 is provided with an outer chute 1-5 corresponding to the outer side of the box frame 1-1, the middle upper part of the rear short side of the box frame 1-1 is provided with a tail chute 1-3, the bottom of the front short side is provided with an arc chute 1-8, the upper parts of the left and right sides of the front end of the box frame 1-1 are symmetrically provided with a bulge, each bulge is provided with a chute extending to the middle part of the side surface of the box frame 1-1, namely, a chute III 1-7, the bottom of the front side in the box frame 1-1 is provided with a fixed seat 1-11, the top of the fixed seat 1-11 is provided with a fixed hole 1-9, the side surface of the fixed seat 1-11 is provided with a fixed screw hole 1-10, and the bottom of the box frame 1-1 is equidistantly distributed with three water leakage holes 1-12. During the use with each subassembly installation on box body 1, will wait to experiment the fish and place fish head fixed component 2 and fish body centre gripping subassembly 3 in and fix.
The fish head fixing component 2 comprises a support rod 2-1, a support table 2-2, screws 2-3, a sliding rod 2-4, nuts 2-5, wing nuts 2-6 and arc-shaped fixing arms 2-7; the support rod 2-1 penetrates through the fixed seat 1-11, the support rod 2-1 is arranged to be a step cylinder, one end of the support rod 2-1 with a small diameter is connected with the support table 2-2, one end of the support rod 2-1 with a large diameter can slide in the fixed seat 1-11, after the support rod is adjusted to a proper position, the screw 2-3 is screwed through the fixed screw hole 1-10 on the side surface of the fixed seat 1-11, the arc-shaped fixed arm 2-7 is used for pressing the fish head, the left side and the right side of the box body 1 are respectively provided with one arc-shaped fixed arm 2-7, the arc-shaped fixed arm 2-7 is designed to be small and exquisite, the radian is consistent with that of the fish skull, the fish head can be fixed in all directions, the skull surface required by brain control technology research can be exposed outside, the arc-shaped fixed arm 2-7 is in threaded connection with the inner end of the slide rod 2-4 through the nut 2-5, the sliding rods 2-4 are arranged in sliding grooves III 1-7 on two sides of the box body 1 and are locked by wing nuts 2-6, so that the fish head can be adjusted to a proper position to be fixed; in this embodiment, the inner side surfaces of the support platform 2-2 and the arc-shaped fixing arm 2-7 are both provided with cotton layers.
The fish body clamping assembly 3 comprises a connecting seat 3-1 and a clamping plate 3-2 with a cotton layer attached to the inner side, the clamping plate 3-2 is set to be in an arc-shaped outline attached to the side face of the fish body, the inner side of the clamping plate is a sponge layer, the middle of the outer side of the clamping plate 3-2 is fixedly connected with the connecting seat 3-1, and a threaded hole is formed in the outer end face of the connecting seat 3-1. When in use, the clamping plates 3-2 at the two sides of the box body are mutually matched in pairs for clamping the fish body.
The clamping height adjusting assembly comprises a sliding block 4-1, a fixing groove 4-2 and a bolt 4-3, the sliding block 4-1 is correspondingly connected with an inner sliding groove 1-2 in a sliding mode, a through hole and a threaded hole are formed in the surface of the sliding block 4-1, the fixing groove 4-2 is correspondingly connected with an outer sliding groove 1-5 in a sliding mode, the bolt 4-3 penetrates through the fixing groove 4-2 and the sliding groove II1-6 from the outer side of the box body 1 in sequence and then is connected into the threaded hole of the sliding block 4-1, the bolt 4-3 is loosened, the sliding block 4-1 and the fixing groove 4-2 can slide, and the bolt 4-3 is screwed down to fix the sliding block 4-1 and the fixing groove 4-2.
The inner end of the fixed groove 4-2 is fixedly connected with a baffle corresponding to the sliding block 4-1 and is in sliding connection with the outer sliding groove 1-5 through the baffle, two sides of the surface of the baffle are respectively provided with a sliding hole 4-2-1 and a bolt hole 4-2-3, the middle part of the fixed groove 4-2 is provided with a sliding groove 4-2-2, a hole tightly attached to the baffle is used for penetrating through a sliding rod, namely the sliding rod hole 4-2-1, the other hole is used for penetrating through a bolt, namely the bolt hole 4-2-3, and the sliding groove is used for sliding of the sliding rod.
The clamping tightness adjusting assembly 5 comprises an L-shaped sliding rod 5-1 and a fixing nut 5-2, the L-shaped sliding rod 5-1 is installed on the fixing groove 4-2, one end of the L-shaped sliding rod passes through a sliding hole 4-2-1 and a through hole in a sliding block and then is connected with a threaded hole in the outer end face of the connecting seat 3-1, the other end of the L-shaped sliding rod is connected with the sliding groove 4-2-2 in a sliding mode, the connecting position is locked through the fixing nut 5-2, the fixing nut 5-2 is loosened, clamping force of the L-shaped sliding rod 5-1 can be adjusted in a sliding mode, and the fixing nut 5-2 is tightened to fix the L-shaped sliding rod 5-.
The electrode clamping assembly 6 comprises a bolt 6-1, a wing nut 6-2, a rotating rod 6-3, a special-shaped U-shaped clamp 6-4, a clamping bottom plate 6-5, a special-shaped U-shaped clamping rod 6-6, a clamping plate 6-7 and a spring 6-8; the rotating rod 6-3 is arranged on the outer side of the front end of the box body 1, one end of the bolt 6-1 sequentially penetrates through the arc-shaped sliding groove 1-8 and the bottom of the rotating rod 6-3 and then is fixed through the wing nut 6-2, the top of the rotating rod 6-3 penetrates through the special-shaped U-shaped clamp 6-4, the special-shaped U-shaped clamp 6-4 can slide up and down along the rotating rod 6-3 and can rotate around the sliding rod 6-3, the opening end of the special-shaped U-shaped clamp 6-4 is locked through the wing nut 6-2, one side of the special-shaped U-shaped clamp 6-4 is provided with the threaded rod 6-6, the rod 6-6 penetrates through the clamping bottom plate 6-5 and then is fixed through the wing nut 6-2, and the clamping bottom plate 6-5 can rotate around the rod 6-6, the outer side of the clamping bottom plate 6-5 is fixedly connected with the upper end of the clamping plate 6-7 through a wing nut 6-2, two springs 6-8 are arranged between the clamping plate 6-7 and the clamping bottom plate 6-5, and the electrode clamping assembly 6 can realize multi-angle clamping and fixing of experimental devices such as electrodes, neural prostheses and the like.
The invention provides a fixing method for application of a fish brain control technology, which takes carps as an example and comprises the following specific steps:
step S1: selecting healthy adult carp with tail weight of 1.05kg and body length of 34.50cm, placing in eugenol solution, and performing medicated bath anesthesia;
step S2: placing the anesthetized carp into the box body 1, placing the fish head on a support table 2-2 of the fish head fixing component 2, placing the fish body into the fish body clamping component 3, and adjusting the heights of the support table 2-2 and the clamping height adjusting component 4 to reach proper positions so that the skull surface of the carp is parallel to the horizontal plane; screwing and fixing a bolt 2-3 of the fish head fixing component 2 and a bolt 4-3 of the clamping height adjusting component 4; attaching the arc-shaped fixing arm 2-7 to the skull of the fish head, and screwing down the nut 2-5 and the wing nut 2-6 of the fish head fixing component 2 for fixing; according to the size of the fish body, a fixing nut 5-2 for clamping the tightness adjusting assembly 5 is screwed up and fixed to clamp and fix the fish body;
step S3: craniotomy is performed by using a bone milling drill to expose brain tissues;
step S4: placing the brain electrode between the clamping bottom plate 6-5 and the clamping plate 6-7, screwing the wing nut 6-2 on the clamping plate 6-7, adjusting an appropriate brain electrode implantation angle according to the implantation site of brain tissue, screwing the wing nut 6-2 at the bottom of the rotating rod 6-3, slowly pushing the special-shaped U-shaped clamp 6-4 downwards after fixation, and screwing the wing nut 6-2 of the special-shaped U-shaped clamp 6-4 until the brain electrode implantation is finished;
step S5: smearing dental cement between the skull and the brain electrode to fix the brain electrode;
step S6: after the fixing is finished, all the fixing components are loosened, and the carp can be subjected to an off-water electrical stimulation experiment and an underwater electrical stimulation control experiment.
The above-mentioned embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solution of the present invention by those skilled in the art should fall within the protection scope defined by the claims of the present invention without departing from the spirit of the present invention.
Claims (8)
1. A fixing device for fish brain control technology application is characterized in that: the device comprises a box body, a fish head fixing assembly, a fish body clamping assembly, a clamping height adjusting assembly, a clamping tightness adjusting assembly and an electrode clamping assembly; the fish head fixing assembly is connected to the front side of the box body in a sliding manner, the fish body clamping assemblies are symmetrically arranged on the left side and the right side of the interior of the box body, the clamping height adjusting assemblies are respectively arranged on the outer sides of the middle parts of the fish body clamping assemblies, the clamping height adjusting assemblies are connected with the side walls of the box body in a sliding manner, and the clamping tightness adjusting assemblies are arranged at the outer ends of the clamping height adjusting assemblies; the electrode clamping assembly is arranged on the outer side of the front end of the box body;
the box body comprises a box frame, an inner sliding chute, a tail chute, a sliding chute I, an outer sliding chute, a sliding chute II, a fixed seat, a fixed hole, a fixed screw hole, a sliding chute III, an arc-shaped sliding chute and a water leakage hole; interior spout symmetry equipartition is provided with two logical grooves in the both sides in the long limit of box rack in every, spout I and spout II promptly, and every interior spout corresponds the box rack outside and all is provided with outer spout, the well upper portion of box rack one side minor face is provided with the tail groove, and the bottom of opposite side minor face is provided with circular-arc spout, the upper portion symmetry of box rack front end left and right sides is provided with the bellying, be provided with spout III on the bellying, front end bottom is provided with the fixing base in the box rack, the top of fixing base is provided with the fixed orifices, the side of fixing base is provided with fixed screw, the hole equidistance that leaks distributes in the box rack bottom.
2. The fixing device applied to the fish brain control technology in claim 1, wherein: the fish head fixing component comprises a support rod, a support table, a screw, a sliding rod, a nut, a wing nut and an arc-shaped fixing arm; the support rod penetrates through the fixed seat, the support rod is arranged to be a step cylinder, one end with a small diameter is connected with the support table, one end with a large diameter can slide in the fixed hole to adjust the position, the screw is arranged in a fixed screw hole in the side face of the fixed seat, the arc-shaped fixed arms are symmetrically arranged on the left side and the right side of the front end of the box frame and used for pressing the fish head, the radian of the arc-shaped fixed arms is consistent with that of a fish skull, the arc-shaped fixed arms are in threaded connection with the inner ends of the slide rods through nuts, the slide rods are arranged in slide grooves III on the two sides of the box frame, and the wing-shaped nuts are arranged at the outer ends of the joints of the slide rods and the slide grooves III; the inner side surfaces of the support platform and the arc-shaped fixed arm are both provided with cotton layers.
3. The fixing device applied to the fish brain control technology in claim 1, wherein: the fish body clamping assembly comprises a clamping plate and a connecting seat, wherein a cotton layer is attached to the inner side of the clamping plate; the connecting seat is fixed on the lower part of the outer side of the clamping plate, and a threaded hole is formed in the outer end face of the connecting seat.
4. The fixing device applied to the fish brain control technology according to claim 3, wherein: the clamping height adjusting assembly comprises a sliding block, a bolt and a fixing groove; the slider is installed in interior spout department, be provided with a through-hole and a screw hole on the slider, the outer spout department in the box body is installed to the slide bar fixed slot, bolt one end is connected in the threaded hole of slider after passing slide bar fixed slot and box body in proper order from the box body outside.
5. The fixing device applied to the fish brain control technology according to claim 4, wherein: the middle part of the fixing groove is provided with a sliding groove, the inner end of the fixing groove is provided with a baffle corresponding to the sliding block, the baffle is installed at the outer sliding groove, and the baffle is provided with a bolt hole and a sliding hole.
6. The fixing device applied to the fish brain control technology according to claim 5, wherein: the clamping tightness adjusting assembly comprises an L-shaped sliding rod and a fixing nut; the L-shaped sliding rod is installed on the fixing groove, one end of the L-shaped sliding rod penetrates through the sliding hole in the baffle and the through hole in the sliding block and then is fixedly connected with the connecting seat, the other end of the L-shaped sliding rod is slidably connected with the sliding groove in the middle of the fixing groove, and the outer side of the connecting position is locked through the fixing nut.
7. The fixing device applied to the fish brain control technology in claim 1, wherein: the electrode clamping assembly comprises a bolt, a wing nut, a rotating rod, a clamping bottom plate, a clamping plate, a spring and a special-shaped U-shaped clamp; the special-shaped U-shaped clamp is characterized in that the bolt sequentially penetrates through the arc-shaped sliding groove and the bottom of the rotating rod, the bolt is fixed to the bottom of the rotating rod through a wing nut, the top of the rotating rod is connected with the special-shaped U-shaped clamp in a sliding mode, the opening end of the special-shaped U-shaped clamp is locked through the wing nut, the special-shaped U-shaped clamp can rotate around the rotating rod, a rod with threads is arranged on one side of the special-shaped U-shaped clamp, the rod penetrates through the clamping bottom plate and is fixed through the nut, the clamping bottom plate can rotate around the rod of the special-shaped U-shaped clamp, the lower portion of the clamping bottom plate is fixedly connected with the clamping plate through the wing nut, and the spring is connected between the clamping plate and the clamping bottom plate respectively.
8. A fixing method for application of a fish brain control technology is characterized by comprising the following steps:
step S1: selecting a healthy adult carp, and placing the carp in a eugenol solution for medicated bath anesthesia;
step S2: placing the anesthetized carp into the box body, placing the fish head on a supporting table of the fish head fixing component, placing the fish body into the fish body clamping component, and adjusting the heights of the supporting table and the clamping height adjusting component to reach proper positions so that the skull surface of the carp is parallel to the horizontal plane; screwing and fixing the screw of the fish head fixing component and the bolt of the clamping height adjusting component; attaching the arc-shaped fixing arm to the skull of the fish head, and screwing down a nut and a wing nut of the fish head fixing assembly for fixing; according to the size of the fish body, a fixing nut for clamping the elastic component is screwed up and fixed to clamp and fix the fish body;
step S3: craniotomy is performed by using a bone milling drill to expose brain tissues;
step S4: placing the brain electrode between the clamping bottom plate and the clamping plate, screwing the wing nut on the clamping plate, adjusting a proper brain electrode implantation angle according to the implantation site of brain tissue, screwing the wing nut on the lower part of the rotating rod, slowly pushing the special-shaped U-shaped clamp downwards after fixation, and screwing the wing nut on the special-shaped U-shaped clamp until the brain electrode is implanted completely;
step S5: smearing dental cement between the skull and the brain electrode to fix the brain electrode;
step S6: after the fixing is finished, all the fixing and clamping components are loosened, and the carp can be subjected to an off-water electrical stimulation experiment and an underwater electrical stimulation control experiment.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202110402245.7A CN113180873A (en) | 2021-04-14 | 2021-04-14 | Fixing device and method for application of fish brain control technology |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202110402245.7A CN113180873A (en) | 2021-04-14 | 2021-04-14 | Fixing device and method for application of fish brain control technology |
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| CN211796934U (en) * | 2020-01-16 | 2020-10-30 | 山东大学齐鲁医院(青岛) | Neurosurgery nursing restraint device |
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| CN107951047A (en) * | 2017-12-18 | 2018-04-24 | 合肥市脍炙人家家居用品有限责任公司 | A kind of fruit cleaning device for peeling |
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