CN113176360A - Method for separating and detecting seaweed ketose in stingless bee honey - Google Patents
Method for separating and detecting seaweed ketose in stingless bee honey Download PDFInfo
- Publication number
- CN113176360A CN113176360A CN202110731869.3A CN202110731869A CN113176360A CN 113176360 A CN113176360 A CN 113176360A CN 202110731869 A CN202110731869 A CN 202110731869A CN 113176360 A CN113176360 A CN 113176360A
- Authority
- CN
- China
- Prior art keywords
- trehalulose
- stingless bee
- honey
- stingless
- bee honey
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/28—Control of physical parameters of the fluid carrier
- G01N30/30—Control of physical parameters of the fluid carrier of temperature
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/28—Control of physical parameters of the fluid carrier
- G01N30/32—Control of physical parameters of the fluid carrier of pressure or speed
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/28—Control of physical parameters of the fluid carrier
- G01N30/34—Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
- G01N30/74—Optical detectors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/28—Control of physical parameters of the fluid carrier
- G01N30/32—Control of physical parameters of the fluid carrier of pressure or speed
- G01N2030/324—Control of physical parameters of the fluid carrier of pressure or speed speed, flow rate
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Jellies, Jams, And Syrups (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention belongs to the field of detection of effective components in honey, and particularly relates to a method for detecting trehalulose in stingless honey. The method comprises the operation of separating trehalulose in the stingless bee honey to be detected through liquid chromatography; and selecting a Hi-Plex Pb chromatographic column in the liquid chromatographic separation process. The method realizes the separation of the trehalulose in the stingless bee honey for the first time, is simple and quick, and is beneficial to the subsequent large-scale extraction and application of the trehalulose.
Description
Technical Field
The invention belongs to the field of detection of effective components in honey, and particularly relates to a method for detecting trehalulose in stingless honey.
Background
Stingless bees (Stingless bees) belong toFrom the order of hymenoptera (Hymenoptera) Bee family: (Apidae) The Maifeng group (A)Meliponini) Insects, named stingless bees, are mainly living in tropical and subtropical regions due to degeneration of the stinger at the tail part, and are distributed in tropical regions such as Taiwan province, Yunnan province and Hainan province in China. The stingless bee honey is small in size and low in flying capability, is a main pollinator for small plants, can collect juice, plant or insect secretion blooming around a nest, and is stored in a spherical tank made of a mixture of propolis and beeswax, namely the stingless bee honey. Researches find that the stingless bee honey contains rich nutrient components such as polyphenol compounds and the like, has obvious pharmacological activity and medicinal value, such as antioxidation, inflammation diminishing, bacteriostasis, wound healing promotion, diabetes resistance, eye disease treatment, body metabolism regulation, intestinal flora regulation and the like, and therefore can be considered as an important health food and is more and more popular with consumers. The stingless bee honey has sour and sweet taste and high water content, mainly comprises sugar, and accounts for 95-97% of dry weight of carbohydrate.
Trehalulose (alpha-D-glucopyranosyl-1, 1-D-fructose) is a reducing disaccharide, is present in natural common honey in a very small amount and is difficult to synthesize chemically, and only some microorganisms can convert the structure of sucrose (alpha-D-glucopyranosyl-1, 2-D-fructose) to form isomaltulose (isomaltulose/palatinose, alpha-D-glucopyranosyl-1, 6-D-fructofuranose) and Trehalulose[26]. These sucrose isomers have a higher acid stability than sucrose, and may to some extent increase species competitive advantage. Given that sucrose isomers have not been reported to have side effects, these compounds may be ideal alternatives to sucrose. Isomaltulose has a sweetness of 30% of sucrose, is easily crystallized and is used as a solid additive; however, trehalulose has a sweetness of 60% of sucrose, has high water solubility, can be used as a filler, a humectant and a texture enhancer, and is suitable for processing high-dessert products such as jelly, jam and the like. The digestion and absorption of trehalulose are slower than that of sucrose, which reduces the rate of monosaccharide and insulin release into the blood, and can be used in foods and beverages for diabetic patients; and trehalulose is not effective in causing dental cariesThe substrate for the glucosyltransferase of Streptococcus mutans of (1) can inhibit sucrose-dependent adhesion of Streptococcus mutans to a glass surface, significantly reduce dental caries, and prevent dental caries. In addition to its advantages as a food ingredient, the reducibility of trehalulose makes it a good precursor for chemical modification and is also used to make sugar alcohols, as low-calorie sweeteners and biocompatible polymers. The trehalulose has excellent biological functions, but no detection method aiming at the simple operation steps of the trehalulose in the stingless bee honey in China exists at present.
Disclosure of Invention
Aiming at the technical problems in the prior art, the invention provides a simple method for detecting trehalulose in stingless bee honey.
The method comprises the operation of separating trehalulose in the stingless bee honey to be detected by liquid chromatography; and selecting a Hi-Plex Pb chromatographic column in the liquid chromatographic separation process.
The invention finds that the ideal separation of the trehalulose can be realized by selecting the chromatographic column, the separation operation is simple and rapid, the separation effect is good, and a good foundation can be laid for subsequent detection.
The structural formula of the trehalulose is as follows:
preferably, the liquid chromatography separation process uses water as a mobile phase. A Hi-Plex Pb chromatographic column is selected, water is used as a mobile phase, so that the effective separation of the trehalulose from other components can be realized, and the separation effect is poor by using other organic solvents.
Preferably, in the liquid chromatographic separation process, the column temperature is 45-55 ℃.
Preferably, the conditions of the liquid chromatography detection are that the chromatographic column: Hi-Plex Pb, 300X 7.7 mm, 8 μm; mobile phase: pure water; column temperature: 45-55 ℃; pressure: 0.5 to 0.7 kPa. Under the conditions, the rapid and effective separation of the trehalulose from other components can be realized.
Preferably, the stingless bee honey to be detected is pretreated as follows before detection: diluting the stingless bee honey to be detected, and then filtering the stingless bee honey through a nylon filter membrane. By adopting the chromatographic column, the honey does not need to be subjected to complex pretreatment, and the subsequent detection can be realized only by simple dilution and filtration. In general, the membrane is passed through a 0.22 μm nylon filter.
The invention also provides a method for detecting the trehalulose, and the content of the separated trehalulose is preferably detected by a differential refraction method. The invention finds that the differential refraction method has very high accuracy for the detection of trehalulose.
Preferably, the conditions for differential refractometry detection are as follows: the temperature of the differential detector RID-10A is 28-42 ℃; sample introduction amount: 8-15 mul; flow rate: 0.4-0.6 ml/min; single gradient elution time: 27-28 min.
Preferably, the stingless bee honey is honey produced by a yellow vein stingless bee, a top stingless bee or a gore stingless bee.
The invention has the following beneficial effects:
1) the method realizes the separation of the trehalulose in the stingless bee honey for the first time, is simple and quick, and is beneficial to the subsequent large-scale extraction and application of the trehalulose.
2) The invention can accurately detect the trehalulose by combining a differential refraction method.
Drawings
FIG. 1 is a liquid chromatography inspection chart of yellow stingless bee honey of example 1 (sample No. 4 in Table 1);
FIG. 2 is a liquid chromatography image of the top stingless bee honey of example 2 (sample No. 7 in Table 1);
FIG. 3 is a liquid chromatography image of stingless honey of Goldfish of example 3 (sample No. 11 in Table 1);
FIG. 4 is a spectrum of a yellow vein stingless bee honey of example 4 (sample No. 15 in Table 1);
fig. 5 is a spectrum of a yellow vein stingless bee honey of comparative example 1 (sample No. 4 in table 1).
Detailed Description
The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.
Example 1
The embodiment relates to a separation method of stingless bee honey, which comprises the following steps:
1) the method comprises the following steps of (1) carrying out pretreatment on the yellow-streaked stingless bee honey: accurately weighing 0.5 g of sample in a beaker, adding 5 ml of water for dissolving, transferring to a 10 ml volumetric flask, washing the beaker with 3 ml of water, transferring to the same volumetric flask, and fixing the volume with pure water. Taking 1 ml of sample solution by a disposable syringe, and filtering the sample solution by a 0.22 mu m nylon filter membrane into a sample injection vial to prepare sample injection;
2) the conditions for controlling the liquid chromatography detection are as follows: a chromatographic column: Hi-Plex Pb, 300X 7.7 mm, 8 μm; mobile phase: pure water; column temperature: 50 ℃; pressure: 0.6 kPa, and the sample injection detection (the separation result is shown in figure 1).
Example 2
The embodiment relates to a separation method of stingless bee honey, which comprises the following steps:
1) the top stingless bee honey is pretreated as follows: accurately weighing 0.5 g of sample in a beaker, adding 5 ml of water for dissolving, transferring to a 10 ml volumetric flask, washing the beaker with 3 ml of water, transferring to the same volumetric flask, and fixing the volume with pure water. Taking 1 ml of sample solution by a disposable syringe, and filtering the sample solution by a 0.22 mu m nylon filter membrane into a sample injection vial to prepare sample injection;
2) the conditions for controlling the liquid chromatography detection are as follows: a chromatographic column: Hi-Plex Pb, 300X 7.7 mm, 8 μm; mobile phase: pure water; column temperature: 50 ℃; pressure: 0.6 kPa, and the sample injection detection (the separation result is shown in figure 2).
Example 3
The embodiment relates to a separation method of stingless bee honey, which comprises the following steps:
1) the method comprises the following steps of pretreating the Goldia honey: accurately weighing 0.5 g of sample in a beaker, adding 5 ml of water for dissolving, transferring to a 10 ml volumetric flask, washing the beaker with 3 ml of water, transferring to the same volumetric flask, and fixing the volume with pure water. Taking 1 ml of sample solution by a disposable syringe, and filtering the sample solution by a 0.22 mu m nylon filter membrane into a sample injection vial to prepare sample injection;
2) the conditions for controlling the liquid chromatography detection are as follows: a chromatographic column: Hi-Plex Pb, 300X 7.7 mm, 8 μm; mobile phase: pure water; column temperature: 50 ℃; pressure: 0.6 kPa, and the sample injection detection (the separation result is shown in FIG. 3).
Example 4
Compared with the example 1, the method is different only in column temperature and comprises the following steps:
1) the method comprises the following steps of (1) carrying out pretreatment on the yellow-streaked stingless bee honey: accurately weighing 0.5 g of sample in a beaker, adding 5 ml of water for dissolving, transferring to a 10 ml volumetric flask, washing the beaker with 3 ml of water, transferring to the same volumetric flask, and fixing the volume with pure water. Taking 1 ml of sample solution by a disposable syringe, and filtering the sample solution by a 0.22 mu m nylon filter membrane into a sample injection vial to prepare sample injection;
2) the conditions for controlling the liquid chromatography detection are as follows: a chromatographic column: Hi-Plex Ca, 300X 7.7 mm, 8 μm; mobile phase: pure water; flow rate: 0.6 ml/min; column temperature: at 84 ℃; sample introduction amount: 10 μ l. (the separation result is shown in figure 4)
Examples 5 to 7
The embodiment relates to a method for detecting stingless bee honey, which comprises the following steps:
the method comprises the following steps of detecting the trehalulose separated in the embodiment 1-3 by adopting a differential refraction method, wherein the detection conditions of the differential refraction method are as follows: the temperature of the difference detector RID-10A is 40 ℃; sample introduction amount: 10 mu l of the mixture; flow rate: 0.5 ml/min; single gradient elution time: 27.5 min.
Comparative example 1
Compared with the example 1, the selection of the chromatographic column is changed, and Luna 5 mu m NH is adopted2100A, 250X 4.6 mm chromatography column.
The method comprises the following steps: weighing 5 g of stingless bee honey sample to the nearest 0.001 g. Placing the sample into a 50 ml centrifuge tube, adding 30 ml of water, placing the sample into a water bath at the temperature of not more than 60 ℃ after vortex mixing uniformly, completely dissolving the sample, transferring the sample into a 100 ml volumetric flask, washing the centrifuge tube with 10 ml of water for 3 times, transferring the centrifuge tube into the 100 ml volumetric flask, metering the volume to the volume by using acetonitrile, and mixing uniformly. The sample solution was filtered through a 0.22 μm nylon filter into a sample bottle for liquid chromatography.
A chromatographic column: carbohydrate analysis column Luna 5 μm NH 2100A, 250X 4.6 mm; single gradient elution, mobile phase: acetonitrile + water (77+ 23); flow rate: 1.2 mL/min; column temperature: 35 ℃; detector cell temperature: 40 ℃; sample introduction amount: 10 μ L. (the separation result is shown in figure 5)
Examples of the experiments
The experimental results are as follows:
examples 1 to 4 and comparative examples various kinds of honey described below were tested respectively
TABLE 1
The spectra of the detection results in the above tables are not listed, and only representative spectra are given for illustration. As can be seen from fig. 1 to 3, the method of the present invention can ideally separate the trehalulose from other components, as can be seen from fig. 4, separation of the trehalulose from other components can also be achieved after changing the column temperature, but the separation effect is not good, as can be seen from fig. 5, separation of the trehalulose from other components cannot be achieved after changing the chromatography column.
The results of the tests carried out by the methods of examples 5 to 7 are shown in Table 2
TABLE 2
As can be seen from Table 2, after the trehalulose was separated by the methods of examples 1 to 3, the trehalulose was accurately detected by the differential refraction method.
And (3) verifying the accuracy of the separation method:
standard solution:
trehalulose standard solution: diluting the purchased 1 g/ml mother solution of the trehalulose to 150 mg/ml, and injecting a sample to obtain the time of the trehalulose peak emergence.
Accuracy detection
And (3) repeatability experiment: 6 parts of a 50 mg/ml trehalulose standard solution are prepared in parallel, the time of the peak appearance of the 6 parts of standard solution is recorded as 10. mu.l per injection and the Relative Standard Deviation (RSD) is calculated.
And (3) precision experiment: preparing a 50 mg/ml trehalulose standard solution, continuously injecting samples for 6 times, recording the peak emergence time of the standard solution, and calculating the RSD of the peak area.
Stability test: preparing a 50 mg/ml trehalulose standard solution, injecting 10 muL samples in 0 h, 2 h, 4 h, 6 h, 8 h and 10 h respectively, recording the peak appearance time of the standard solution, and calculating the RSD of the peak area.
Recovery rate experiment: the method comprises the steps of adding trehalulose standard substances with different concentrations into high fructose syrup serving as a sample for recovery, accurately weighing 4 parts of 0.5000 g of high fructose syrup, adding 5 ml, 2.5 ml, 1 ml and 0.5 ml of trehalulose standard substances with the concentration of 100 mg/ml respectively, supplementing water to a system with the concentration of 10 ml, obtaining the final trehalulose solution with the concentrations of 50 mg/ml, 25 mg/ml, 10 mg/ml and 5 mg/ml respectively, taking 1 ml of sample solution with a disposable injector, filtering the sample solution with a 0.22 mu m nylon filter membrane into a sample injection vial, injecting 10 mu L of sample, and calculating the standard addition recovery rate of trehalulose. The results of the repeatability, precision, stability experiments are shown in table 3:
TABLE 3
As can be seen in Table 3, after 6 parts of the trehalulose standard solution of 50 mg/ml is injected, the RSD of the peak area is 0.14%, which indicates that the extraction method has good repeatability; after the same trehalulose standard sample is injected, the RSD of the peak area is 0.18 percent, which shows that the precision of the chromatographic method is good; after three portions of the trehalulose standard substance with the same concentration are measured in different time periods, the RSD of the peak area is 0.94%, which shows that the chromatographic method has good stability within 10 h.
The recovery test results are shown in table 4:
TABLE 4
The results in Table 4 show that the standard recovery rate of the trehalulose with different concentrations in the high fructose corn syrup is 93.5-95.71%, and the RSD is less than 0.61%, which indicates that the method has better recovery rate and accurate and reliable results.
Although the invention has been described in detail hereinabove by way of general description, specific embodiments and experiments, it will be apparent to those skilled in the art that many modifications and improvements can be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Claims (5)
1. A method for separating trehalulose from stingless bee honey is characterized by comprising the operation of separating the trehalulose from the stingless bee honey to be detected through liquid chromatography; the conditions of the liquid chromatography detection are that a chromatographic column: Hi-Plex Pb, 300X 7.7 mm, 8 μm; mobile phase: pure water; column temperature: 45-55 ℃; pressure: 0.5 to 0.7 kPa.
2. The separation method according to claim 1, wherein the stingless bee honey to be detected is pretreated before detection as follows: diluting the stingless bee honey to be detected, and then filtering the stingless bee honey through a nylon filter membrane.
3. A method for detecting trehalulose in stingless bee honey, which is characterized in that the content of the trehalulose obtained by the method of claim 1 or 2 is detected by a differential refraction method.
4. The detection method according to claim 3, wherein the conditions for the differential refractometry detection are: the temperature of the differential detector is 38-42 ℃; flow rate: 0.4-0.6 ml/min; single gradient elution time: 27-28 min.
5. The detection method according to claim 3 or 4, wherein the stingless bee honey is a honey produced by a yellow-striped stingless bee, a top stingless bee or a Goldfish stingless bee.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110731869.3A CN113176360A (en) | 2021-06-30 | 2021-06-30 | Method for separating and detecting seaweed ketose in stingless bee honey |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110731869.3A CN113176360A (en) | 2021-06-30 | 2021-06-30 | Method for separating and detecting seaweed ketose in stingless bee honey |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113176360A true CN113176360A (en) | 2021-07-27 |
Family
ID=76927978
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110731869.3A Pending CN113176360A (en) | 2021-06-30 | 2021-06-30 | Method for separating and detecting seaweed ketose in stingless bee honey |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113176360A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104017028A (en) * | 2014-06-23 | 2014-09-03 | 齐鲁工业大学 | Method of separating isomaltulose and trehalulose from isomaltulose mother liquor |
| CN111747999A (en) * | 2020-06-24 | 2020-10-09 | 李宪臻 | Method for separating and preparing trehalulose from sucrose isomerase enzymatic hydrolysate |
-
2021
- 2021-06-30 CN CN202110731869.3A patent/CN113176360A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104017028A (en) * | 2014-06-23 | 2014-09-03 | 齐鲁工业大学 | Method of separating isomaltulose and trehalulose from isomaltulose mother liquor |
| CN111747999A (en) * | 2020-06-24 | 2020-10-09 | 李宪臻 | Method for separating and preparing trehalulose from sucrose isomerase enzymatic hydrolysate |
Non-Patent Citations (2)
| Title |
|---|
| RAJNI KAMBOJ 等: "Sugar profile and rheological behaviour of four different Indian honey varieties", 《J FOOD SCI TECHNOL》 * |
| 覃怀怀 等: "海藻酮糖及其分离方法初探", 《轻工科技》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Zhu et al. | Nutritional and antioxidant properties of non-centrifugal cane sugar derived from membrane clarified juice | |
| CN109125258B (en) | Preparation method of strong loquat syrup | |
| CN103142720B (en) | Black raspberry extract is preparing the application in antidiabetic medicine | |
| CN108938654A (en) | A kind of injection preparation of anemoside B4 | |
| Famojuro | Effect of cashew apple juice (Anacardium occidentale L.) on hematology and spleen of gentamicin induced injury in albino rats | |
| CN104435034B (en) | A kind of arasaponin and preparation method thereof | |
| CN113176360A (en) | Method for separating and detecting seaweed ketose in stingless bee honey | |
| Kliewer | The sugars of grapevines. II. identification and seasonal changes in the concentration of several trace sugars in vitis vinifera | |
| CN104473159B (en) | A kind of silvervine effervescent agent and preparation method thereof and application | |
| CN111227144B (en) | Inonotus obliquus composite beverage and preparation method thereof | |
| CN108771690B (en) | A Balanophora japonica L extract with blood sugar or blood lipid reducing effect, and its preparation method and application | |
| CN103142474B (en) | With the composition and method of making the same that high purity bilobalide B is active component | |
| CN106674299B (en) | Carbene glycosides compound and its preparation method and application | |
| CN106916065A (en) | The method that high-purity chlorogenic acid is prepared from radix bardanae | |
| CN106512018A (en) | Honey refining method suitable for honey boluses | |
| CN103585226B (en) | A kind of preparation method of Herba Elephantopi Mollis extract and application thereof | |
| CN107334775A (en) | THPA is preparing the application in treating atherosclerosis medicine | |
| Chitravel et al. | Antidiabetic potential of Biophytum sensitivum whole plant extracts in STZ induced diabetic rats | |
| CN113845602A (en) | Red ginseng polysaccharide composition and preparation method and application thereof | |
| CN105125482A (en) | Puerarin injection and quality control method thereof | |
| CN116173085B (en) | Acanthopanax senticosus extract and preparation method and application thereof | |
| KR100562525B1 (en) | Composition containing a polysaccharide fraction isolated from cordyceps militaris for preventing and treating diabetes mellitus | |
| CN110664953B (en) | Processing method of radix stemonae | |
| CA3036500C (en) | Plant-soaked solution comprising tagatose, and method for producing same | |
| CN106608899B (en) | The preparation method and medical usage of 20 (R)-protopanaxatriols and derivative |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210727 |
|
| RJ01 | Rejection of invention patent application after publication |