CN113176324A - Borax boric acid buffer solution and application thereof in electrophoresis - Google Patents
Borax boric acid buffer solution and application thereof in electrophoresis Download PDFInfo
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- CN113176324A CN113176324A CN202110503957.8A CN202110503957A CN113176324A CN 113176324 A CN113176324 A CN 113176324A CN 202110503957 A CN202110503957 A CN 202110503957A CN 113176324 A CN113176324 A CN 113176324A
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- 229910021538 borax Inorganic materials 0.000 title claims abstract description 70
- 239000004328 sodium tetraborate Substances 0.000 title claims abstract description 70
- 235000010339 sodium tetraborate Nutrition 0.000 title claims abstract description 70
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 title claims abstract description 64
- 239000004327 boric acid Substances 0.000 title claims abstract description 64
- 239000007853 buffer solution Substances 0.000 title claims abstract description 50
- 238000001962 electrophoresis Methods 0.000 title claims abstract description 35
- 210000002966 serum Anatomy 0.000 claims abstract description 33
- 238000000246 agarose gel electrophoresis Methods 0.000 claims abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- 239000000872 buffer Substances 0.000 claims description 14
- 239000012153 distilled water Substances 0.000 claims description 11
- 239000011543 agarose gel Substances 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 8
- 229920002301 cellulose acetate Polymers 0.000 claims description 7
- 238000005303 weighing Methods 0.000 claims description 5
- 229920000936 Agarose Polymers 0.000 claims description 4
- BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 claims description 4
- 238000002791 soaking Methods 0.000 claims description 3
- 108090001030 Lipoproteins Proteins 0.000 abstract description 13
- 102000004895 Lipoproteins Human genes 0.000 abstract description 13
- 229920006221 acetate fiber Polymers 0.000 abstract description 11
- 239000012528 membrane Substances 0.000 abstract description 11
- 238000002474 experimental method Methods 0.000 abstract description 10
- 230000000694 effects Effects 0.000 abstract description 4
- 239000000126 substance Substances 0.000 abstract description 4
- 238000011160 research Methods 0.000 abstract description 3
- 238000011161 development Methods 0.000 abstract description 2
- 102000004169 proteins and genes Human genes 0.000 abstract description 2
- 108090000623 proteins and genes Proteins 0.000 abstract description 2
- 229940125717 barbiturate Drugs 0.000 description 8
- HNYOPLTXPVRDBG-UHFFFAOYSA-N barbituric acid Chemical compound O=C1CC(=O)NC(=O)N1 HNYOPLTXPVRDBG-UHFFFAOYSA-N 0.000 description 8
- 239000000243 solution Substances 0.000 description 8
- 239000000499 gel Substances 0.000 description 7
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 4
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 4
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 4
- 239000007982 barbital buffer Substances 0.000 description 4
- 239000011521 glass Substances 0.000 description 4
- 239000011734 sodium Substances 0.000 description 4
- 229910052708 sodium Inorganic materials 0.000 description 4
- 102000009027 Albumins Human genes 0.000 description 3
- 108010088751 Albumins Proteins 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 229960000796 barbital sodium Drugs 0.000 description 3
- FTOAOBMCPZCFFF-UHFFFAOYSA-N barbitone sodium Natural products CCC1(CC)C(=O)NC(=O)NC1=O FTOAOBMCPZCFFF-UHFFFAOYSA-N 0.000 description 3
- 238000004043 dyeing Methods 0.000 description 3
- 230000000147 hypnotic effect Effects 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- RGHFKWPGWBFQLN-UHFFFAOYSA-M sodium;5,5-diethylpyrimidin-3-ide-2,4,6-trione Chemical compound [Na+].CCC1(CC)C([O-])=NC(=O)NC1=O RGHFKWPGWBFQLN-UHFFFAOYSA-M 0.000 description 3
- 238000010186 staining Methods 0.000 description 3
- 102000006734 Beta-Globulins Human genes 0.000 description 2
- 108010087504 Beta-Globulins Proteins 0.000 description 2
- 108010017384 Blood Proteins Proteins 0.000 description 2
- 102000004506 Blood Proteins Human genes 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 108010044091 Globulins Proteins 0.000 description 2
- 102000006395 Globulins Human genes 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- YCUVUDODLRLVIC-UHFFFAOYSA-N Sudan black B Chemical compound C1=CC(=C23)NC(C)(C)NC2=CC=CC3=C1N=NC(C1=CC=CC=C11)=CC=C1N=NC1=CC=CC=C1 YCUVUDODLRLVIC-UHFFFAOYSA-N 0.000 description 2
- 229960000583 acetic acid Drugs 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 208000019425 cirrhosis of liver Diseases 0.000 description 2
- 230000005611 electricity Effects 0.000 description 2
- 108010074605 gamma-Globulins Proteins 0.000 description 2
- 239000012362 glacial acetic acid Substances 0.000 description 2
- 229910052742 iron Inorganic materials 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000000932 sedative agent Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
- 206010067484 Adverse reaction Diseases 0.000 description 1
- 108010004103 Chylomicrons Proteins 0.000 description 1
- 108010010234 HDL Lipoproteins Proteins 0.000 description 1
- 102000015779 HDL Lipoproteins Human genes 0.000 description 1
- 102000008100 Human Serum Albumin Human genes 0.000 description 1
- 108091006905 Human Serum Albumin Proteins 0.000 description 1
- 108010007622 LDL Lipoproteins Proteins 0.000 description 1
- 102000007330 LDL Lipoproteins Human genes 0.000 description 1
- 108010052285 Membrane Proteins Proteins 0.000 description 1
- 102000018697 Membrane Proteins Human genes 0.000 description 1
- 229910004835 Na2B4O7 Inorganic materials 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 108010062497 VLDL Lipoproteins Proteins 0.000 description 1
- 230000006838 adverse reaction Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 239000003618 borate buffered saline Substances 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- UQGFMSUEHSUPRD-UHFFFAOYSA-N disodium;3,7-dioxido-2,4,6,8,9-pentaoxa-1,3,5,7-tetraborabicyclo[3.3.1]nonane Chemical compound [Na+].[Na+].O1B([O-])OB2OB([O-])OB1O2 UQGFMSUEHSUPRD-UHFFFAOYSA-N 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 239000003326 hypnotic agent Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000004081 narcotic agent Substances 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 238000007639 printing Methods 0.000 description 1
- 229940001470 psychoactive drug Drugs 0.000 description 1
- 239000004089 psychotropic agent Substances 0.000 description 1
- 230000001624 sedative effect Effects 0.000 description 1
- 239000012192 staining solution Substances 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention relates to a borax boric acid buffer solution and application thereof in electrophoresis, belonging to the technical field of biology. The pH of borax boric acid buffer solution is 8.6, and the ionic strength is 0.05, 0.06 or 0.075, and the borax boric acid buffer solution is applied to electrophoresis. The borax boric acid buffer solution has the advantages that the borax boric acid buffer solution is prepared by using two chemicals of borax and boric acid, and the two chemicals are easy to purchase and have low price. The pH of the buffer solution is easy to adjust, the ionic strength is easy to calculate, and the buffer solution is suitable for carrying out serum acetate fiber membrane electrophoresis and serum lipoprotein electrophoresis experiments. The borax boric acid buffer solution has a very good effect, correct protein bands are distinguished by serum acetate fiber membrane electrophoresis, lipoprotein bands are distinguished by serum lipoprotein agarose gel electrophoresis, the bands are clear, clear and complete, and support is provided for normal development of experimental research and experimental teaching.
Description
Technical Field
The invention belongs to the field of biotechnology, relates to a buffer solution for electrophoresis, and particularly relates to a borax boric acid buffer solution and application thereof in serum acetate fiber membrane and lipoprotein agarose gel electrophoresis.
Background
The serum cellulose acetate membrane electrophoresis uses a barbital sodium/barbital buffer solution with the pH of 8.6 and the ionic strength of 0.06, and uses a barbital sodium/barbital buffer solution with the pH of 8.6 and the ionic strength of 0.05 and 0.075 respectively for serum lipoprotein agarose gel electrophoresis. The serum cellulose acetate membrane electrophoresis experiment is very important in the biochemistry experiment teaching of medical college, and can divide plasma protein into: albumin, alpha1Globulin, alpha2Globulin, beta-globulin, and gamma-globulin; students can master the principle of electrophoresis technology, conditions which the electrophoresis must have, factors which influence the electrophoresis, and other aspects of knowledge. In addition, the method has important significance for mastering the classification and content of serum protein, and helps students to understand the role of inversion of albumin-globulin ratio in diagnosis of liver cirrhosis (normal human albumin/globulin is 1.5-2.5: 1, and liver cirrhosis albumin/globulin is 0.5: 1). Serum agarose gel electrophoresisThe experiment can lead the students to master the classification, the composition and the functions of the serum lipoproteins, and has very important significance for the students to master the metabolism of the serum lipoproteins. For years, the barbiturate sodium/barbiturate buffer solution is used in both experiments, and the barbiturate sodium is a short-term barbiturate hypnotic, so that the central inhibition effect can achieve the sedative and hypnotic effects along with the increase of the dose. Barbiturate has been clinically used as a hypnotic or sedative drug, but has been eliminated because of its weak action, many adverse reactions, insecurity and easy dependence. Because barbiturate sodium and barbiturate are narcotics and psychotropic drugs, they cannot be reused. Without the corresponding buffer solution of pH and ionic strength, the two experiments cannot be set up again for students, and the students lose the two biochemical experiments for verifying theoretical knowledge. It is therefore important to find alternative buffers to the sodium/barbiturate buffer.
Disclosure of Invention
The invention provides a borax boric acid buffer solution and application thereof in electrophoresis, aiming at establishing proper pH and ionic strength by using borax and boric acid to replace a barbital sodium/barbital buffer solution, and using the borax boric acid buffer solution in serum cellulose acetate membranes and serum lipoprotein agarose gel electrophoresis.
The technical scheme adopted by the invention is as follows:
a borax boric acid buffer having a pH of 8.6 and an ionic strength of 0.05, 0.06 or 0.075.
A method for preparing borax boric acid buffer solution with pH 8.6 and ionic strength of 0.06 comprises the following steps: 7.63 g of borax and 3.30 g of boric acid are taken and dissolved by adding distilled water, and the final volume is 1 liter.
A method for preparing borax boric acid buffer solution with pH 8.6 and ionic strength of 0.05 comprises the following steps: 6.36 g of borax and 2.75 g of boric acid are taken and dissolved by adding distilled water, and the final volume is 1 liter.
A method for preparing borax boric acid buffer solution with pH 8.6 and ionic strength of 0.075 comprises the following steps: 9.536 g of borax and 4.123 g of boric acid are taken and dissolved by adding distilled water, and the final volume is 1 liter.
The borax boric acid buffer solution disclosed by the invention is applied to electrophoresis.
The invention soaks the acetate fiber film into borax boric acid buffer solution with pH 8.6 and ionic strength 0.06 for 10-20 minutes.
The invention uses borax boric acid buffer solution with pH 8.6 and ionic strength 0.05 to prepare 0.035% agarose gel, and the steps are: weighing 0.035g agarose, adding 100ml borax boric acid buffer solution, placing in a microwave oven for dissolving, subpackaging in a triangular flask, and storing in a refrigerator.
The borax boric acid buffer solution with the pH value of 8.6 and the ionic strength of 0.075 is used as the buffer solution in the serum agarose gel electrophoresis tank.
The invention has the advantages that: the borax boric acid buffer solution is prepared by using two chemicals of borax and boric acid, and the two chemicals are easy to purchase and low in price. The pH value of the buffer solution is easy to adjust, the ionic strength is easy to calculate, and the buffer solution is suitable for carrying out serum acetate fiber membrane electrophoresis and serum lipoprotein electrophoresis experiments, wherein a borax boric acid buffer solution with 0.06 ionic strength and pH 8.6 is prepared and can be used for the serum acetate fiber membrane electrophoresis experiments, and a borax boric acid buffer solution with 0.05 ionic strength, 0.075 ionic strength and pH 8.6 is prepared and used for serum lipoprotein agarose gel electrophoresis, and can completely replace a barbital buffer solution. In addition, the borax boric acid buffer solution can also be used in other life science experimental researches with different pH values and different ionic strengths according to requirements. The borax boric acid buffer solution has a very good effect, and correct protein bands are distinguished by serum acetate fiber membrane electrophoresis, while lipoprotein bands are distinguished by serum lipoprotein agarose gel electrophoresis. The strip is clear, clear and complete, the expected experimental effect is achieved, and support is provided for normal development of experimental research and experimental teaching.
Drawings
FIG. 1 is a photograph of a serum cellulose acetate membrane electrophoresis using borax boric acid buffer of 0.06 ionic strength and pH 8.6 in accordance with the present invention;
FIG. 2 is a serum agarose gel electrophoresis image using borax boric acid buffer of 0.05 and 0.075 ionic strength and pH 8.6 of the present invention.
Detailed Description
Borax Na2B4O7·10H2O, molecular weight is 381.43, borax volatilizes crystal water and needs to be stored in a bottle with a plug; boric acid H3BO3The molecular weight is 61.84.
Example 1 borax borate buffered saline with pH 8.6, ionic strength 0.06:
dissolving 7.63 g of borax and 3.30 g of boric acid in distilled water to obtain a solution with a final volume of 1 liter, wherein the concentration of the borax is 0.02M and the concentration of the boric acid is 0.0534M;
ionic strength 1/2 × (2 × 0.02 × 1)2+0.02×22)＝0.06
Example 2 Borax Borate buffer of pH 8.6 and Ionic Strength 0.05 was prepared
Dissolving 6.36 g of borax and 2.75 g of boric acid in distilled water to obtain a solution with a final volume of 1 liter, wherein the concentration of the borax is 0.0167M and the concentration of the boric acid is 0.0445M;
ionic strength 1/2 × (2 × 0.0167 × 1)2+0.0167×22)＝0.05
Example 3 Borax Borate buffer of pH 8.6 and Ionic Strength 0.075 was formulated
9.536 g of borax and 4.123 g of boric acid are taken and dissolved by distilled water, the final volume is 1 liter, the concentration of the borax is 0.025M, and the concentration of the boric acid is 0.0667M;
ionic strength 1/2 × (2 × 0.025 × 1)2+0.025×22)＝0.075
Example 4 serum acetate Membrane electrophoresis
Reagents and instrumentation:
1. a cellulose acetate film of 2cm × 8cm or 1.5cm × 8 cm;
2. borax boric acid buffer, pH 8.6, ionic strength 0.06;
3. dyeing liquid: weighing 0.5g of amino black 10B, dissolving in 50ml of methanol, and then adding 10ml of glacial acetic acid and 40ml of distilled water;
4. rinsing liquid: 45ml of 95 percent ethanol, 5ml of glacial acetic acid and 50ml of distilled water are added and mixed evenly;
5. an electrophoresis apparatus and an electrophoresis tank;
and (3) experimental operation:
1. acetate fiber film treatment: soaking the acetate fiber film in borax boric acid buffer solution for 10-20 minutes, taking out the acetate fiber film, and slightly absorbing the redundant buffer solution by using filter paper;
2. sample application: sucking a drop of serum on a glass plate, dipping the serum by using a sample application knife, flatly and straightly printing a steel port at a position 2cm away from one end of an acetate fiber film, and then placing a sample application end at the cathode end of an electrophoresis tank to be bridged by 4 layers of filter paper;
3. electrifying; the voltage is 110V, the electricity is conducted for 45 minutes, after the electricity is cut off, the film is taken out of the electrophoresis tank, and the filter paper is used for slightly absorbing excessive water;
4. staining and elution: soaking the electrophoretic cellulose acetate film in dyeing liquor for 5 min, washing with rinsing liquor for several times to obtain clear 5 color bands of albumin and alpha1Globulin, alpha2Globulin, beta-globulin and gamma-globulin, which can be quantified using a spectrophotometer, see FIG. 1.
Example 5 serum lipoprotein agarose gel electrophoresis
Reagent and apparatus
1. pH of borax boric acid buffer solution is 8.6, the ionic strength for electrophoresis is 0.075, and the ionic strength for agarose gel preparation is 0.05;
2. agarose gel: weighing 0.035g of agarose, adding borax boric acid buffer solution with pH of 8.6, ionic strength of 0.05 and 100ml, dissolving in boiling water bath, subpackaging in test tubes, and storing in refrigerator;
3. dyeing liquid: 0.1g of Sudan black is dissolved in 10m of isopropanol 1;
4. an electrophoresis apparatus and an electrophoresis tank.
Experimental procedures
1. Agarose gel: weighing 0.035g of agarose, adding borax boric acid buffer solution with pH of 8.6, ionic strength of 0.05 and 100ml, dissolving in a microwave oven, subpackaging in a triangular flask, and preserving in a refrigerator;
2. pre-staining of serum: mixing 0.2m1 serum with 0.02ml Sudan black staining solution in a small test tube, placing in a 37 ℃ water bath for staining for 30 minutes, then centrifuging for 5 minutes at 2000rpm, and taking the supernatant for later use;
3. preparation of agarose gel plates: heating and melting the prepared agarose gel in a microwave oven, placing a small iron rod at a position about 2cm away from one end of a gel plate, sucking gel solution by using a suction pipe, pouring the gel solution into glass slides, uniformly pouring each glass slide by about 2.5ml onto the glass slides, standing for about 20 minutes, and then solidifying;
4. spot-wise pre-stained serum: after the gel plate is solidified, sucking out the small iron bar by using a magnet, carefully sucking water in the reserved small groove by using filter paper, sucking about 12 mu 1 of pre-dyed serum by using a microsyringe, and injecting the pre-dyed serum into the small groove of the gel plate;
5. electrophoresis: the gel plate added with serum is parallelly placed in an electrophoresis tank, the sample end is placed at a cathode, two three layers of gauze are soaked in borax boric acid buffer solution with the pH value of 8.6 and the ionic strength of 0.075 and are lightly pasted at the two ends of the gel plate, the lower end of the gauze is immersed in the borax boric acid buffer solution with the ionic strength of 0.075 in the electrophoresis tank, a power supply is switched on, the voltage is 110 volts, the time is 45 minutes, zones separated after power failure can be seen and are respectively alpha-lipoprotein, pre-beta-lipoprotein, beta-lipoprotein and chylomicron remained in a spot sample hole, and the figure 2 shows.
Claims (8)
1. A borax boric acid buffer solution is characterized in that: borax borate buffer pH 8.6, ionic strength 0.05, 0.06 or 0.075.
2. The method for preparing borax boric acid buffer solution according to claim 1, wherein the borax boric acid buffer solution with pH 8.6 and ionic strength 0.06 is prepared by the following steps: 7.63 g of borax and 3.30 g of boric acid are taken and dissolved by adding distilled water, and the final volume is 1 liter.
3. The method for preparing borax boric acid buffer solution according to claim 1, wherein the borax boric acid buffer solution with pH 8.6 and ionic strength 0.05 is prepared by the following steps: 6.36 g of borax and 2.75 g of boric acid are taken and dissolved by adding distilled water, and the final volume is 1 liter.
4. The method for preparing borax boric acid buffer solution according to claim 1, wherein the borax boric acid buffer solution with pH 8.6 and ionic strength 0.075 is prepared by the following steps: 9.536 g of borax and 4.123 g of boric acid are taken and dissolved by adding distilled water, and the final volume is 1 liter.
5. The use of the borax boric acid buffer according to claim 1 in electrophoresis.
6. The use of the borax boric acid buffer according to claim 5 in electrophoresis, wherein: soaking the cellulose acetate film in borax boric acid buffer solution with pH 8.6 and ionic strength of 0.06 for 10-20 min.
7. The use of the borax boric acid buffer according to claim 5 in electrophoresis, wherein: the procedure for preparing a 0.035% agarose gel using borax borate buffer, pH 8.6, ionic strength 0.05, was: weighing 0.035g agarose, adding 100ml borax boric acid buffer solution, placing in a microwave oven for dissolving, subpackaging in a triangular flask, and storing in a refrigerator.
8. The use of the borax boric acid buffer according to claim 5 in electrophoresis, wherein: borax boric acid buffer solution with pH 8.6 and ionic strength of 0.075 is used as the buffer solution in the serum agarose gel electrophoresis tank.
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| CN110007095A (en) * | 2019-05-06 | 2019-07-12 | 江苏硕世生物科技股份有限公司 | A kind of dengue virus NS 1 antigen test strip, kit and preparation method thereof |
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| CN110007095A (en) * | 2019-05-06 | 2019-07-12 | 江苏硕世生物科技股份有限公司 | A kind of dengue virus NS 1 antigen test strip, kit and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| "预染脂旦白琼脂电泳实验方法及其临床上的应用", 广东医学, no. 01, pages 43 - 47 * |
| 唐秋艳 等 主编: "《免疫诊断试剂实用技术》", 31 August 2009, 海洋出版社, pages: 225 * |
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