CN113176131A - Secondary anti-blue dyeing machine - Google Patents
Secondary anti-blue dyeing machine Download PDFInfo
- Publication number
- CN113176131A CN113176131A CN202110453684.0A CN202110453684A CN113176131A CN 113176131 A CN113176131 A CN 113176131A CN 202110453684 A CN202110453684 A CN 202110453684A CN 113176131 A CN113176131 A CN 113176131A
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- China
- Prior art keywords
- water
- dyeing machine
- blue
- signal output
- heating coil
- Prior art date
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- Granted
Links
- 238000004043 dyeing Methods 0.000 title claims abstract description 36
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 92
- 238000010438 heat treatment Methods 0.000 claims abstract description 45
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 34
- 238000007789 sealing Methods 0.000 claims description 11
- 238000001914 filtration Methods 0.000 claims 10
- 238000002347 injection Methods 0.000 claims 2
- 239000007924 injection Substances 0.000 claims 2
- BGOFCVIGEYGEOF-UJPOAAIJSA-N helicin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC=CC=C1C=O BGOFCVIGEYGEOF-UJPOAAIJSA-N 0.000 claims 1
- 241000220317 Rosa Species 0.000 description 17
- 210000000805 cytoplasm Anatomy 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 230000001575 pathological effect Effects 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000007490 hematoxylin and eosin (H&E) staining Methods 0.000 description 2
- 238000009434 installation Methods 0.000 description 2
- 230000003749 cleanliness Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- YQGOJNYOYNNSMM-UHFFFAOYSA-N eosin Chemical compound [Na+].OC(=O)C1=CC=CC=C1C1=C2C=C(Br)C(=O)C(Br)=C2OC2=C(Br)C(O)=C(Br)C=C21 YQGOJNYOYNNSMM-UHFFFAOYSA-N 0.000 description 1
- 239000008236 heating water Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
Abstract
The invention discloses a secondary anti-blue dyeing machine, which comprises a dyeing machine main body, wherein a blue returning reagent cylinder for filling a reagent into a dyeing cylinder is arranged in the dyeing machine main body, the dyeing machine main body also comprises a temperature control device for maintaining the temperature of the blue returning reagent cylinder, the temperature control device comprises a controller, a temperature sensor, a water tank, an electric heater and a spiral heating coil, a water outlet and a water return port are respectively arranged on two sides of the water tank, a water pump is arranged at the water outlet, the electric heater is arranged in the water tank, the heating coil is arranged around the bluing reagent tank, two ends of the heating coil are respectively connected with the water outlet and the water return port, the temperature sensor is arranged in the blue returning reagent cylinder, the signal input end of the controller is connected with the signal output end of the temperature sensor, and a first signal output end of the controller is connected with a signal input end of the water pump, and a second signal output end of the controller is connected with a signal output end of the electric heater.
Description
Technical Field
The invention relates to the technical field of medical detection equipment, in particular to a secondary anti-blue dyeing machine.
Background
The high-quality pathological HE staining piece is the key for pathological result diagnosis of pathologists. How to dye a high-quality dyeing piece, how to make the tissue morphological structure contrast clear, the nucleus clear and the cytoplasm bright is a big problem in pathological dyeing. Conventional HE staining only needs one blue returning operation, and the prepared HE tablet often has the conditions of unobvious cytoplasm, unclear nucleoplasm boundary and eosin superstaining.
Disclosure of Invention
In order to solve the problems in the prior art, the invention provides a secondary anti-blue dyeing machine.
The technical scheme adopted by the invention is as follows:
a secondary anti-blue dyeing machine comprises a dyeing machine main body, wherein a blue returning reagent cylinder for filling a reagent into a dyeing cylinder is arranged in the dyeing machine main body, the secondary anti-blue dyeing machine also comprises a temperature control device for maintaining the temperature of the blue returning reagent cylinder, the temperature control device comprises a controller, a temperature sensor, a water tank, an electric heater and a spiral heating coil, a water outlet and a water return port are respectively arranged on two sides of the water tank, a water pump is arranged at the water outlet, the electric heater is arranged in the water tank, the heating coil is arranged around the bluing reagent tank, two ends of the heating coil are respectively connected with the water outlet and the water return port, the temperature sensor is arranged in the blue returning reagent cylinder, the signal input end of the controller is connected with the signal output end of the temperature sensor, and a first signal output end of the controller is connected with a signal input end of the water pump, and a second signal output end of the controller is connected with a signal output end of the electric heater.
Preferably, a pressure relief valve is arranged at the top of the water tank.
Preferably, the top of the water tank is provided with a water filling port.
Preferably, two heating coils are arranged around the outer circumference of the blue returning reagent cylinder, and the two heating coils are arranged in a staggered and reverse rotating manner; the inlet of one heating coil is positioned above, and the outlet is positioned below; the inlet of the other heating coil is positioned below, and the outlet is positioned below; inlets of the two heating coils are connected in parallel and then connected with a water outlet, and outlets of the two heating coils are connected in parallel and then connected with a water return port.
Preferably, be equipped with level sensor in the water tank, the water filling port is equipped with the solenoid valve, level sensor's signal output part is connected to the second signal input part of controller, the signal input part of solenoid valve is connected to the third signal output part of controller.
Preferably, a filter is arranged between the water return port and the heating coil.
Preferably, the filter includes rose box and filter screen, the both sides wall of rose box communicates with return water mouth and heating coil respectively, the vertical middle part of locating the rose box of filter screen.
Preferably, the filter screen can be dismantled with the rose box and be connected, the periphery of filter screen is equipped with the frame, the top of rose box articulates there is the case lid, be equipped with the hasp between case lid and the rose box, the inside wall of rose box is equipped with the recess respectively corresponding to the both sides limit and the base of filter screen, be equipped with the sealing strip in the recess, the filter screen is located in the recess and sealed through the sealing strip between the frame of filter screen and the recess, the bottom surface of case lid is equipped with the layering that is used for compressing tightly the top frame of filter screen.
Preferably, the filter screen is of an inverted triangular structure, and the groove is of a V-shaped structure matched with two side edges of the filter screen.
Preferably, the two sides of the top frame of the filter screen are respectively provided with a convex strip.
Preferably, be equipped with turbidity sensor in the rose box, turbidity sensor is located the filter screen and is close to the one side of return water mouth, the top surface of case lid is equipped with the display screen, turbidity sensor's signal output part connects the signal input part of display screen.
The invention has the beneficial effects that: the temperature in the bluing reagent cylinder can be maintained at about 50 ℃ by the temperature control device, the HE sheet is bluing twice by utilizing the warm water bluing principle, the dyeing quality of the HE sheet is improved, the HE sheet dyed by the bluing twice has obvious nucleus and cytoplasm, clear nucleus and cytoplasm and distinct contrast, and is beneficial to the judgment of a clinician on the patient result.
Drawings
FIG. 1 is a schematic structural diagram of an embodiment of the present invention;
FIG. 2 is a schematic diagram of a connection structure of a controller according to an embodiment of the present invention;
FIG. 3 is a schematic top view of the case cover according to the embodiment of the present invention;
FIG. 4 is a schematic view of the interior of the filter box according to the embodiment of the present invention;
FIG. 5 is a schematic diagram of the right side view of the inside of the filter box according to the embodiment of the present invention;
reference numerals: 1. dyeing machine main part, 2, return blue reagent jar, 3, controller, 4, temperature sensor, 5, water tank, 6, electric heater, 7, heating coil, 8, water pump, 9, relief valve, 10, water filling port, 11, level sensor, 12, solenoid valve, 13, rose box, 14, case lid, 15, filter screen, 16, recess, 17, sealing strip, 18, layering, 19, sand grip, 20, turbidity sensor, 21, display screen.
Detailed Description
Embodiments of the present invention will be described in detail below with reference to the accompanying drawings.
Examples
As shown in fig. 1-5, a secondary anti-blue dyeing machine comprises a dyeing machine main body 1, a blue returning reagent tank 2 for filling a reagent into the dyeing tank is arranged in the dyeing machine main body 1, and the secondary anti-blue dyeing machine further comprises a temperature control device for maintaining the temperature of the blue returning reagent tank 2, wherein the temperature control device comprises a controller 3, a temperature sensor 4, a water tank 5, an electric heater 6 and a spiral heating coil 7, a water outlet and a water return port are respectively arranged on two sides of the water tank 5, a water pump 8 is arranged at the water outlet, the electric heater 6 is arranged in the water tank 5, the heating coil 7 is arranged around the blue returning reagent tank 2, two ends of the heating coil 7 are respectively connected with the water outlet and the water return port, the temperature sensor 4 is arranged in the blue returning reagent tank 2, a signal input end of the controller 3 is connected with a signal output end of the temperature sensor 4, a first signal output end of the controller 3 is connected with a signal input end of the water pump 8, and a second signal output end of the controller 3 is connected with a signal output end of the electric heater 6.
The invention circularly supplies hot water to the heating coil 7 by heating water in the water tank 5 through the electric heater 6, and the heating coil 7 arranged around the side wall of the blue returning reagent cylinder 2 heats the blue returning reagent cylinder 2 through the circulating hot water, so that the temperature in the blue returning reagent cylinder 2 is maintained at about 50 ℃.
The temperature in the blue returning reagent cylinder 2 is detected by the temperature sensor 4, the detected temperature is sent to the controller 3, and the controller 3 controls the start and stop of the electric heater 6 and the water pump 8 according to the real-time temperature in the blue returning reagent cylinder 2 so as to maintain the temperature in the blue returning reagent cylinder 2 and save the electric quantity.
And a pressure release valve 9 is arranged at the top of the water tank 5.
When the electric heater 6 heats the water in the water tank 5, if the internal pressure of the water tank 5 is too high, the pressure can be released by the relief valve 9.
The top of the water tank 5 is provided with a water filling port 10.
During use, water in the water tank 5 generates steam due to heating, loss is generated when the pressure release valve 9 releases pressure, loss is also generated due to other reasons, and water can be supplemented through the water filling port 10.
Two heating coils 7 are wound outside the blue returning reagent tank 2, and the two heating coils are arranged in a staggered and reverse rotating mode; the inlet of one of the heating coils 7 is located above and the outlet is located below; the inlet of the other heating coil 7 is positioned below, and the outlet is positioned below; inlets of the two heating coils 7 are connected in parallel and then connected with a water outlet, and outlets of the two heating coils 7 are connected in parallel and then connected with a water return port.
The heating of returning blue reagent jar 2 is carried out to hot water through two mutual reverse transport of heating coil 7, and the high temperature end and the low temperature end of one of them heating coil 7 correspond low temperature end and the high temperature end of another heating coil 7 respectively, make to return blue reagent jar 2 and be heated more evenly from top to bottom, return the temperature in the blue reagent jar 2 more balanced.
Be equipped with level sensor 11 in the water tank 5, water filling port 10 is equipped with solenoid valve 12, level sensor 11's signal output part is connected to the second signal input part of controller 3, solenoid valve 12's signal input part is connected to the third signal output part of controller 3.
The level of the water in the water tank 5 is detected by the liquid level sensor 11, when the water level is low, the electromagnetic valve 12 is opened to fill water into the water tank 5, and when the water is filled to a certain level, the electromagnetic valve 12 is closed.
And a filter is arranged between the water return port and the heating coil 7.
The circulating water in the water tank 5 is filtered by the filter, so that high cleanliness of the circulating water is ensured.
The filter includes rose box 13 and filter screen 15, the both sides wall of rose box 13 communicates with return water mouth and heating coil 7 respectively, filter screen 15 is vertical to be located the middle part of rose box 13.
The filter box 13 is matched with the filter screen 15, so that the structure is simple, the installation is convenient, and the cost is lower.
The sealing is formed by matching the groove 16, the sealing strip 17 and the frame of the filter screen 15, so that water flowing through the filter box 13 from the heating coil 7 is prevented from flowing into a water return port without being filtered, and the sealing reliability of the frame at the bottom of the filter screen 15 can be ensured by the arrangement of the pressing strip 18.
The filter screen 15 is of an inverted triangular structure, and the groove 16 is of a V-shaped structure matched with two side edges of the filter screen 15.
The V-shaped groove 16 makes the filter screen 15 more convenient to install, and compared with the grooves 16 with two vertical sides, the friction force between the frame of the filter screen 15 and the sealing strips 17 in the grooves 16 is greatly reduced during installation.
Convex strips 19 are respectively arranged on two sides of the top frame of the filter screen 15.
The provision of the ribs 19 facilitates easy hand-lifting of the filter screen 15 during replacement of the filter screen 15.
Be equipped with turbidity sensor 20 in the rose box 13, turbidity sensor 20 is located the one side that filter screen 15 is close to the return water mouth, the top surface of case lid 14 is equipped with display screen 21, turbidity sensor 20's signal output part connects display screen 21's signal input part.
The above-mentioned embodiments only express the specific embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention.
Claims (10)
1. The utility model provides a secondary anti-blue dyeing machine, includes dyeing machine main part (1), be equipped with in dyeing machine main part (1) and be used for returning blue reagent jar (2) to the in-cylinder filling reagent of dyeing, its characterized in that still includes the temperature control device who is used for maintaining and returns blue reagent jar (2) temperature, temperature control device includes controller (3), temperature sensor (4), water tank (5), electric heater (6) and is spiral helicine heating coil (7), the both sides of water tank (5) are equipped with delivery port and return water mouth respectively, delivery port department is equipped with water pump (8), in water tank (5) are located to electric heater (6), heating coil (7) encircle to return blue reagent jar (2) setting, delivery port and return water mouth are connected respectively to the both ends of heating coil (7), temperature sensor (4) are located and are returned blue reagent jar (2), a first signal input end of the controller (3) is connected with a signal output end of the temperature sensor (4), a first signal output end of the controller (3) is connected with a signal input end of the water pump (8), and a second signal output end of the controller (3) is connected with a signal output end of the electric heater (6); two heating coils (7) are wound on the outer ring of the blue returning reagent cylinder (2), and the two heating coils are arranged in a staggered and reverse rotating mode; the inlet of one heating coil (7) is positioned above, and the outlet is positioned below; the inlet of the other heating coil (7) is positioned below, and the outlet is positioned below; inlets of the two heating coils (7) are connected in parallel and then connected with a water outlet, and outlets of the two heating coils (7) are connected in parallel and then connected with a water return port.
2. A secondary reverse blue dyeing machine according to claim 1, characterized in that the top of said tank (5) is provided with a pressure relief valve (9).
3. A secondary reverse blue dyeing machine according to claim 2, characterized in that the top of said tank (5) is provided with a water injection port (10).
4. A secondary anti-blue dyeing machine according to claim 3, characterized in that said tank (5) is provided with a level sensor (11), said water injection port (10) is provided with a solenoid valve (12), a second signal input of said controller (3) is connected to a signal output of said level sensor (11), and a third signal output of said controller (3) is connected to a signal input of said solenoid valve (12).
5. A secondary anti-blue dyeing machine according to claim 4, characterized in that a filter is provided between said water return port and heating coil (7).
6. A secondary anti-blue dyeing machine according to claim 5, characterized in that said filter comprises a filtering tank (13) and a filtering screen (15), both side walls of said filtering tank (13) are respectively communicated with said water return port and said heating coil (7), said filtering screen (15) is vertically arranged in the middle of said filtering tank (13).
7. The secondary anti-blue dyeing machine according to claim 6, characterized in that the filter screen (15) is detachably connected with the filter box (13), a frame is arranged on the periphery of the filter screen (15), a box cover (14) is hinged to the top of the filter box (13), a lock catch is arranged between the box cover (14) and the filter box (13), grooves (16) are respectively arranged on the inner side wall of the filter box (13) corresponding to the two side edges and the bottom edge of the filter screen (15), sealing strips (17) are arranged in the grooves (16), the filter screen (15) is arranged in the grooves (16), the frame of the filter screen (15) and the grooves (16) are sealed through the sealing strips (17), and a pressing strip (18) for pressing the top frame of the filter screen (15) is arranged on the bottom surface of the box cover (14).
8. A secondary reverse blue dyeing machine according to claim 7, characterized in that said filtering screen (15) presents an inverted triangular structure and said groove (16) presents a V-shaped structure matching with the two sides of the filtering screen (15).
9. A secondary reverse blue dyeing machine according to claim 8, characterized in that said filtering net (15) is provided with raised strips (19) on both sides of its top border.
10. A secondary anti-blue dyeing machine according to claim 9, characterized in that a turbidity sensor (20) is arranged in said filtering box (13), said turbidity sensor (20) is arranged on the side of the filtering net (15) close to the water return port, a display screen (21) is arranged on the top surface of said box cover (14), and the signal output end of said turbidity sensor (20) is connected with the signal input end of the display screen (21).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110453684.0A CN113176131B (en) | 2021-04-26 | 2021-04-26 | Secondary anti-blue dyeing machine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110453684.0A CN113176131B (en) | 2021-04-26 | 2021-04-26 | Secondary anti-blue dyeing machine |
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| Publication Number | Publication Date |
|---|---|
| CN113176131A true CN113176131A (en) | 2021-07-27 |
| CN113176131B CN113176131B (en) | 2024-03-29 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110453684.0A Active CN113176131B (en) | 2021-04-26 | 2021-04-26 | Secondary anti-blue dyeing machine |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113176131B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101087303B1 (en) * | 2011-05-06 | 2011-11-29 | 주식회사 셀앤텍바이오 | Apparatus for staining specimens |
| CN205175793U (en) * | 2015-10-30 | 2016-04-20 | 杨杰 | Pathological tissue dyeing machine |
| WO2018115928A1 (en) * | 2016-12-20 | 2018-06-28 | Diapath S.P.A. | Disposable container for stains or reagents for staining machines of biological material and staining system comprising said container |
| CN212807805U (en) * | 2020-08-04 | 2021-03-26 | 湖北阳光神琦医用科技有限公司 | Heating constant-temperature reagent tank of DNA dyeing machine |
-
2021
- 2021-04-26 CN CN202110453684.0A patent/CN113176131B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101087303B1 (en) * | 2011-05-06 | 2011-11-29 | 주식회사 셀앤텍바이오 | Apparatus for staining specimens |
| CN205175793U (en) * | 2015-10-30 | 2016-04-20 | 杨杰 | Pathological tissue dyeing machine |
| WO2018115928A1 (en) * | 2016-12-20 | 2018-06-28 | Diapath S.P.A. | Disposable container for stains or reagents for staining machines of biological material and staining system comprising said container |
| CN212807805U (en) * | 2020-08-04 | 2021-03-26 | 湖北阳光神琦医用科技有限公司 | Heating constant-temperature reagent tank of DNA dyeing machine |
Non-Patent Citations (1)
| Title |
|---|
| 林永兴: ""返蓝现象"与碘量法滴定时效", 中国造纸, no. 05, 25 May 1993 (1993-05-25), pages 64 * |
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| CN113176131B (en) | 2024-03-29 |
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