CN113171812B - Method for quickly sterilizing superclean workbench - Google Patents
Method for quickly sterilizing superclean workbench Download PDFInfo
- Publication number
- CN113171812B CN113171812B CN202110437176.3A CN202110437176A CN113171812B CN 113171812 B CN113171812 B CN 113171812B CN 202110437176 A CN202110437176 A CN 202110437176A CN 113171812 B CN113171812 B CN 113171812B
- Authority
- CN
- China
- Prior art keywords
- light source
- photosensitizer
- sterilization
- infrared light
- znpc
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L9/00—Supporting devices; Holding devices
- B01L9/02—Laboratory benches or tables; Fittings therefor
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/08—Radiation
- A61L2/085—Infrared radiation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/08—Radiation
- A61L2/10—Ultra-violet radiation
Abstract
The invention relates to a method for quickly sterilizing a superclean benchThe method comprises the following steps: preparation of ZnPc4Photosensitizer, loading infrared light source on super clean bench, and mixing ZnPc4The photosensitizer is sprayed or smeared on an ultra-clean working table, and an ultraviolet light source and an infrared light source are turned on to perform sterilization. Infrared sterilization depends on photosensitizer ZnPc4According to the photodynamic antibacterial principle, the photosensitizer can excite ground state oxygen to generate singlet oxygen under the irradiation of red light, and the singlet oxygen can generate obvious phototoxicity killing effect on bacteria, viruses, harmful cells and the like. Before only needing degerming at every turn, evenly spraying the photosensitizer on superclean bench surface with the watering can, opening ultraviolet and infrared light source simultaneously, under the supplementary degerming effect of photosensitizer, can show the degerming speed that accelerates superclean bench to for experiment save time, improve superclean bench's utilization ratio, this degerming method has good application prospect.
Description
Technical Field
The invention relates to the technical field of sterilization, in particular to a method for quickly sterilizing a superclean workbench.
Background
A clean bench (also called clean bench) is widely used in the fields of modernization industry, photoelectric industry, bio-pharmaceuticals, scientific research and experiment, etc. The principle of the superclean bench is that in a specific space, indoor air is primarily filtered through a prefilter, is pressed into a static pressure box through a small centrifugal fan, is secondarily filtered through an air high-efficiency filter, and clean airflow blown out from an air outlet surface of the air high-efficiency filter has certain and uniform section air speed, so that original air in a working area can be removed, dust particles and biological particles are taken away, and a sterile high-cleanness working environment is formed. In the repeated use process of the clean bench, the front and back operators are easy to cross-pollute. Therefore, the ultra-clean workbench needs about 30 minutes of ultraviolet lamp irradiation for disinfection before working each time, so that the relatively clean and sterile experiment table surface can be ensured, and the accuracy of the experiment is ensured. At present, most superclean bench all adopts the ultraviolet lamp to shine the principle of disinfecting and carries out the degerming, and the effect of degerming relies on the luminous power and the illumination time of ultraviolet lamp, often needs the longer time just can guarantee abundant degerming.
The prior art has the following disadvantages: the existing ultra-clean workbench mainly depends on ultraviolet lamp irradiation for sterilization, the sterilization mode is single, and due to the limited efficiency of ultraviolet sterilization, the sterilization effect can be achieved only by enough light power and dosage, and the sterilization effect on some high-activity bacteria, infectious bacteria and the like is limited.
Disclosure of Invention
The object of the present invention is to provide a new method for rapid sterilization of superclean benches to improve the above background art problems. Therefore, on the basis of ultraviolet sterilization, the infrared sterilization is added to realize efficient and comprehensive sterilization effect.
In order to achieve the above purpose, the present invention provides the following technical solution, a method for rapid sterilization of a clean bench, the method comprising the following steps:
s1, preparation of ZnPc4A photosensitizer;
s2, loading an infrared light source on the superclean workbench;
s3, mixing ZnPc4The photosensitizer is sprayed or smeared on an ultra-clean working table, and an ultraviolet light source and an infrared light source are turned on to perform sterilization.
Infrared sterilization depends on photosensitizer ZnPc4(Zinc phthalocyanine, molecular formula: C)32H16N8Zn), the photosensitizer can excite ground state oxygen to generate singlet oxygen under the irradiation of red light, and the singlet oxygen can generate obvious phototoxicity killing effect on bacteria, viruses, harmful cells and the like. Before only needing degerming at every turn, evenly spraying the photosensitizer on superclean bench surface with the watering can, opening ultraviolet and infrared light source simultaneously, under the supplementary degerming effect of photosensitizer, can show the degerming speed that accelerates superclean bench to for experiment save time, improve superclean bench's utilization ratio, this degerming method has good application prospect.
As a further improvement of the method for rapid sterilization of the superclean bench of the invention, ZnPc is prepared4The solvent used for the photosensitizer is 5% ethanol water solution.
ZnPc prepared as a further improvement of the method for rapid sterilization of superclean benches of the present invention4The concentration of the photosensitizer is 30-70 nM/mL (nanomoles per milliliter).
ZnPc prepared as a further improvement of the method for rapid sterilization of superclean benches of the present invention4The concentration of photosensitizer was 50 nM/mL.
As a further improvement of the method for rapid sterilization of the clean bench of the invention, in step S1, the prepared ZnPc is used4The photosensitizer was transferred to a brown spray bottle for storage.
As a further improvement of the method for rapid sterilization of an ultra clean bench according to the present invention, in step S2, the infrared light source is an LED infrared light source.
As a further improvement of the method for quickly sterilizing the superclean bench, in step S2, the maximum emission wavelength of the infrared light source is 650-680 nm.
As a further improvement of the method for rapid sterilization of clean bench of the present invention, in step S2, the maximum emission wavelength of the infrared light source is 660nm, which can maximize the antibacterial effect of the excited photosensitizer.
As a further improvement of the method for quickly sterilizing the superclean bench, in the step S3, the ultraviolet light source and the infrared light source are turned on for sterilizing for 1-10 min.
As a further improvement of the method for rapid sterilization by using an ultra-clean bench, in step S3, the sterilization time is 5min by using an ultraviolet light source and an infrared light source.
Compared with the prior art, the invention has the beneficial effects that: adopts a mode of combining ultraviolet sterilization and infrared sterilization, utilizes the sterilization effect of the ultraviolet sterilization on common bacteria and viruses within the wavelength range of 240-280 nm, and utilizes the photosensitizer ZnPc4The two modes simultaneously carry out sterilization, can obviously accelerate the sterilization rate of the superclean bench, thereby saving time for experiments, improving the utilization rate of the superclean bench and having good application prospect.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be clearly and completely described below, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.
The method and results for rapid sterilization of superclean benches of the present invention are summarized as follows:
(1) preparation of ZnPc4The photosensitizer is adjusted to be 30-70 nM/mL (nanomole per mL, solvent is 5% ethanol water solution), and is transferred into a brown spray bottle.
(2) And (3) loading an LED infrared light source on the clean bench, wherein the maximum emission wavelength of the LED infrared light source is 650-680 nm.
(3) Before sterilization, ZnPc is firstly used4The photosensitizer is uniformly sprayed on the ultra-clean working table, and ultraviolet and infrared light sources are turned on simultaneously to perform sterilization.
(4) After the degerming, carry out antibiotic effect evaluation, the contrast is found that this scheme is used for ultraviolet, the infrared sterilization technique of jointly of superclean bench degerming can show and improve degerming effect, shorten degerming time.
In the method, infrared sterilization depends on a photosensitizer ZnPc4(Zinc phthalocyanine, molecular formula: C)32H16N8Zn), the photosensitizer can excite ground state oxygen to generate singlet oxygen under the irradiation of red light, and the singlet oxygen can generate obvious phototoxicity killing effect on bacteria, viruses, harmful cells and the like. Before only needing degerming at every turn, evenly spraying the photosensitizer on superclean bench surface with the watering can, opening ultraviolet and infrared light source simultaneously, under the supplementary degerming effect of photosensitizer, can show the degerming speed that accelerates superclean bench to for experiment save time, improve superclean bench's utilization ratio, this degerming method has good application prospect.
Example 1
(1) Preparation of ZnPc4The photosensitizer, adjusted to a concentration of 50nM/mL (nanomoles per mL, solvent 5% aqueous ethanol), was transferred to a brown spray bottle.
(2) An LED infrared light source is loaded on the clean bench, and the maximum emission wavelength of the LED infrared light source is 660 nm.
(3) Before sterilizationFirstly ZnPc4And uniformly spraying the photosensitizer on an ultra-clean working table, simultaneously turning on ultraviolet and infrared light sources for sterilization, and counting the killing rate of staphylococcus aureus by taking the staphylococcus aureus as a test object.
(4) After the degerming, carry out antibiotic effect evaluation, the contrast is found that this scheme is used for ultraviolet, the infrared combined sterilization technique of superclean bench degerming can show and improve degerming effect, shorten degerming time, and the result statistics is in table 1.
Example 2
(1) Preparation of ZnPc4The photosensitizer, adjusted to a concentration of 30nM/mL (nanomoles per mL, solvent 5% aqueous ethanol), was transferred to a brown spray bottle.
(2) An LED infrared light source is loaded on the clean bench, and the maximum emission wavelength of the LED infrared light source is 650 nm.
(3) Before sterilization, ZnPc is firstly used4And uniformly spraying the photosensitizer on an ultra-clean working table, simultaneously turning on ultraviolet and infrared light sources for sterilization, and counting the killing rate of staphylococcus aureus by taking the staphylococcus aureus as a test object.
(4) After the degerming, carry out antibiotic effect evaluation, the contrast is found that this scheme is used for ultraviolet, the infrared combined sterilization technique of superclean bench degerming can show and improve degerming effect, shorten degerming time, and the result statistics is in table 1.
Example 3
(1) Preparation of ZnPc4The photosensitizer, adjusted to a concentration of 70nM/mL (nanomoles per mL, solvent 5% aqueous ethanol), was transferred to a brown spray bottle.
(2) An LED infrared light source is loaded on the clean bench, and the maximum emission wavelength of the LED infrared light source is 680 nm.
(3) Before sterilization, ZnPc is firstly used4And uniformly spraying the photosensitizer on an ultra-clean workbench surface, simultaneously turning on ultraviolet and infrared light sources for sterilization, and counting the killing rate of staphylococcus aureus by taking the staphylococcus aureus as a test object.
(4) After the degerming, carry out antibiotic effect evaluation, the contrast is found that this scheme is used for ultraviolet, the infrared combined sterilization technique of superclean bench degerming can show and improve degerming effect, shorten degerming time, and the result statistics is in table 1.
Comparative example 1
And (3) turning on an ultraviolet light source, sterilizing the ultra-clean working table, and counting the killing rate of the staphylococcus aureus by taking the staphylococcus aureus as a test object.
After the degerming, carry out antibiotic effect evaluation, the contrast finds that this scheme is used for the ultraviolet degerming technique degerming effect of superclean bench degerming not as good as embodiment 1 ~ 3, and its degerming time is longer, and the result statistics is in table 1.
TABLE 1 comparison of the sterilizing effects of examples 1 to 3 and comparative example 1
As can be seen from Table 1, the sterilization effect of the infrared and ultraviolet sterilization modes of examples 1-3 is obviously better than that of the sterilization mode of comparative example 1 which uses only ultraviolet, wherein the sterilization effect of example 1 is the best.
The method combines ultraviolet and infrared sterilization, realizes multi-mode quick sterilization of the superclean bench, obviously shortens sterilization time, and improves the utilization rate of the superclean bench.
The method introduces photodynamic sterilization on the basis of ultraviolet sterilization, and realizes efficient and comprehensive sterilization by means of the efficient antibacterial action of the photosensitizer ZnPc4 on various bacteria.
The above description is only a part of examples and test examples of the present invention, not all examples and test examples, and the detailed description of the examples and test examples of the present invention is not intended to limit the scope of the claimed invention, but merely represents selected examples and test examples of the present invention. All other embodiments and test examples obtained by a person skilled in the art without any inventive work are within the scope of the present invention.
Claims (7)
1. A method for rapid sterilization of a clean bench is characterized by comprising the following steps:
s1, preparation of ZnPc4A photosensitizer; preparation of ZnPc4The concentration of the photosensitizer is 30-70 nM/mL;
s2, loading an infrared light source on the superclean workbench; the maximum emission wavelength of the infrared light source is 650-680 nm;
s3, mixing ZnPc4Spraying or coating a photosensitizer on an ultra-clean working table, and turning on an ultraviolet light source and an infrared light source to perform sterilization; opening an ultraviolet light source and an infrared light source for sterilization for 1-10 min; the pathogen removed was staphylococcus aureus.
2. The method for rapid sterilization of clean bench according to claim 1, characterized in that ZnPc is prepared4The solvent used for the photosensitizer is 5% ethanol water solution.
3. The method for the rapid sterilization of superclean benches as claimed in claim 2, characterized in that ZnPc is prepared4The concentration of photosensitizer was 50 nM/mL.
4. The method for rapid sterilization of clean bench as claimed in claim 1, wherein in step S1, the prepared ZnPc is used4The photosensitizer was transferred to a brown spray bottle for storage.
5. The method for rapid sterilization of clean bench according to claim 1, wherein in step S2, the infrared light source is LED infrared light source.
6. The method for rapid sterilization of clean bench according to claim 1, wherein in step S2, the maximum emission wavelength of the infrared light source is 660 nm.
7. The method for rapid sterilization of clean bench according to claim 1, wherein the sterilization using the ultraviolet light source and the infrared light source is performed for 5min in step S3.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110437176.3A CN113171812B (en) | 2021-04-22 | 2021-04-22 | Method for quickly sterilizing superclean workbench |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110437176.3A CN113171812B (en) | 2021-04-22 | 2021-04-22 | Method for quickly sterilizing superclean workbench |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113171812A CN113171812A (en) | 2021-07-27 |
| CN113171812B true CN113171812B (en) | 2022-05-10 |
Family
ID=76924672
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110437176.3A Active CN113171812B (en) | 2021-04-22 | 2021-04-22 | Method for quickly sterilizing superclean workbench |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113171812B (en) |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104127906A (en) * | 2014-08-07 | 2014-11-05 | 青岛阳光动力生物医药技术有限公司 | Photodynamic antibacterial unit equipment for air purification |
| CN106604728A (en) * | 2014-09-09 | 2017-04-26 | 普尔伯格有限公司 | Compositions for photodynamic control of infection |
| US11039616B2 (en) * | 2016-05-10 | 2021-06-22 | North Carolina State University | Photodynamic compositions, methods of making, and uses thereof |
| CN208810122U (en) * | 2018-09-26 | 2019-05-03 | 南京林业大学 | Superclean bench |
| CN210097703U (en) * | 2019-05-23 | 2020-02-21 | 太原工业学院 | Superclean bench of high-efficient sterilization |
| CN112452683A (en) * | 2020-10-19 | 2021-03-09 | 赵梓俨 | Infrared antibacterial coating and preparation method thereof |
-
2021
- 2021-04-22 CN CN202110437176.3A patent/CN113171812B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN113171812A (en) | 2021-07-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| TW200514613A (en) | Air treatment device | |
| CN111869976A (en) | Gauze mask degassing unit is used in gauze mask production | |
| CN113171812B (en) | Method for quickly sterilizing superclean workbench | |
| CN201052279Y (en) | Hand-hold grape disease germicidal lamp | |
| CN111632334A (en) | Disinfectant, and preparation method and application thereof | |
| CN204593640U (en) | A kind of environmental protection and energy saving negative ion air-cleaner | |
| CN107233923B (en) | Material for decomposing formaldehyde and organic gas through photodynamic catalysis and preparation method thereof | |
| CN212777806U (en) | Large-flow ultraviolet air sterilizer | |
| CN104654729A (en) | Multifunctional illuminating system for refrigerator | |
| CN204460919U (en) | A kind of multifunctional refrigerator illuminator | |
| CN113041370A (en) | Tunnel conveying type ultraviolet new coronavirus sterilizing and disinfecting equipment | |
| CN208809151U (en) | A kind of files book disinfection cabinet with circulated air | |
| RU2316366C2 (en) | Photodynamic method for inactivating bacteria | |
| CN211781644U (en) | Indoor air purification and disinfection machine | |
| CN108410307A (en) | A kind of water-and acrylate nano controlled-release insecticidal and antibacterial coating | |
| CN110917838B (en) | Light/oxygen catalyst purifying agent and preparation method and application thereof | |
| CN111282432B (en) | Preparation method of long-acting antibacterial aldehyde-removing odor-removing treatment liquid and product thereof | |
| CN212204875U (en) | Air purifier with sterilizing and formaldehyde removing functions | |
| CN210097703U (en) | Superclean bench of high-efficient sterilization | |
| CN104549271B (en) | Mineral wool board and preparation method thereof | |
| CN213159890U (en) | Comprehensive environment-friendly air circulation disinfection equipment | |
| CN114887667A (en) | Preparation method of novel photocatalytic air purification material | |
| CN101480500B (en) | Intensified safety air machine for disinfection with omnidirectional reflexion of ultraviolet ray | |
| CN206786883U (en) | A kind of online high-pressure ion dynamic bactericidal purifying exhaust blower | |
| CN209679085U (en) | A kind of antibacterial lamp of ultraviolet sterilization |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |