CN113156634A - Multi-scene real-time application ultra-portable optical tweezers - Google Patents
Multi-scene real-time application ultra-portable optical tweezers Download PDFInfo
- Publication number
- CN113156634A CN113156634A CN202110372705.6A CN202110372705A CN113156634A CN 113156634 A CN113156634 A CN 113156634A CN 202110372705 A CN202110372705 A CN 202110372705A CN 113156634 A CN113156634 A CN 113156634A
- Authority
- CN
- China
- Prior art keywords
- laser
- lens group
- ultra
- lens
- optical tweezers
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000012576 optical tweezer Methods 0.000 title claims abstract description 40
- 238000005286 illumination Methods 0.000 claims abstract description 43
- 239000002245 particle Substances 0.000 claims abstract description 12
- 230000003287 optical effect Effects 0.000 claims description 38
- 238000003384 imaging method Methods 0.000 claims description 15
- 239000004065 semiconductor Substances 0.000 claims description 8
- 210000001747 pupil Anatomy 0.000 claims description 7
- 241000276498 Pollachius virens Species 0.000 claims description 4
- 239000006059 cover glass Substances 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 4
- 230000001681 protective effect Effects 0.000 claims description 2
- 238000010586 diagram Methods 0.000 description 9
- 238000011160 research Methods 0.000 description 7
- 230000000694 effects Effects 0.000 description 5
- 235000012149 noodles Nutrition 0.000 description 5
- 238000013461 design Methods 0.000 description 4
- 239000004973 liquid crystal related substance Substances 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- -1 polypropylene Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/32—Micromanipulators structurally combined with microscopes
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
- G02B21/08—Condensers
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/361—Optical details, e.g. image relay to the camera or image sensor
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Engineering & Computer Science (AREA)
- Multimedia (AREA)
- Microscoopes, Condenser (AREA)
Abstract
The invention provides a multi-scene real-time application ultra-portable optical tweezers, which comprises a laser, a laser collimation module, a laser beam expansion module, a dichroic reflector, a lens group serving as an objective lens, a miniature electric sample stage, a condenser lens group, an illumination light source, a photographic eyepiece, a CMOS camera, a control panel and a display screen, wherein the control panel is arranged on the control panel; emitting laser light of a single wavelength by a laser; the laser collimation module performs laser collimation; the laser beam expanding module expands laser beams; the dichroic mirror reflects laser light of a desired wavelength while transmitting light beams of other wavelengths; a lens group serving as an objective lens focuses laser to the position of the miniature electric sample stage; the illumination light source and the condenser lens group provide illumination light for the sample cell; the information of the particles is obtained by the photographing ocular lens and the CMOS camera; the display screen observes the target object in the sample pool; the control panel adjusts the miniature electric sample stage. The invention has the characteristics of small volume, integrated structure, convenient carrying and transportation, convenient operation by adopting a customized light path, and multi-scene real-time application due to a totally enclosed operating environment.
Description
Technical Field
The invention relates to the field of optical tweezers, in particular to an ultra-portable optical tweezers with multi-scene real-time application.
Background
The optical tweezers use the gradient force formed by the spatial variation of the light field intensity to stably capture the particles at the strongest position of the light field, namely the focal position of the light beam. When the laser beam moves, the particles can be driven to move together, and the precise control of the particles is realized. The optical tweezers technology is gradually applied to the biological and medical research fields, such as cell, virus and bacteria manipulation, because the optical tweezers have the characteristics of no damage, non-invasiveness and the like to the manipulated particles, the size of the particles captured by the optical tweezers is usually in the micrometer to nanometer order, and the size of biological cells and macromolecules is also usually in the dozen nanometers to dozens of micrometers. The traditional optical tweezers are constructed based on an optical microscope system, and focus laser beams through an optical microscope objective lens, and capture and operate particles by forming an optical trap through a gradient force field near a focusing center. The main structure of the traditional optical tweezers system is a high-precision optical microscope, the instrument is large in size, the system is complex and heavy, the price is high, the operation interface is complex, the requirement on operation skills is high, the degree of freedom of sample movement is small, the capture range of the optical tweezers is limited, and the scene use is limited.
Disclosure of Invention
The invention mainly aims to overcome the defects in the prior art, provides the ultra-portable optical tweezers for multi-scene real-time application, has the characteristics of small volume, integrated structure, convenience in carrying and transportation, convenience in operation by adopting a customized optical path and capability of multi-scene real-time application due to a fully-closed operating environment, and provides an advanced and portable research tool for scientific research in the field of biomedical science.
The invention adopts the following technical scheme:
the ultra-portable optical tweezers for multi-scene real-time application is characterized by comprising a laser, a laser collimation module, a laser beam expansion module, a dichroic reflector, a lens group serving as an objective lens, a miniature electric sample stage, a condenser lens group, an illumination light source, a photographic eyepiece, a CMOS camera, a control panel and a display screen; specifically, a laser of a single wavelength is emitted by a laser; performing laser collimation through a laser collimation module; performing laser beam expansion through a laser beam expansion module; reflecting laser light of a desired wavelength while transmitting light beams of other wavelengths through a dichroic mirror; carrying out laser focusing to a micro electric sample stage through a lens group serving as an objective lens; providing illumination light for the sample cell through an illumination light source and a condenser lens group; acquiring information of the particles through a photographic eyepiece and a CMOS camera; observing the target object in the sample pool through a display screen; the micro electric sample stage is adjusted by the control board to change the sample position until the target object is captured by the optical trap, and then other operations are carried out on the target object.
Specifically, the central wavelength of the semiconductor laser is 675nm, the output power is 150mW, and the semiconductor laser is packaged by TO, and the divergence angle θ | | ═ 7.0 ° in the sagittal direction and the divergence angle θ | ═ 15 ° in the meridional direction.
Specifically, the laser collimation module adopts two vertically-arranged plano-convex cylindrical lenses, the surface type of the cylindrical lenses is an elliptical type, and the laser collimation module collimates laser so that the divergence angle theta | | | in the sagittal direction is 0.1776 °, the divergence angle theta | | in the meridional direction is 0.0745 °, and quasi-parallel light with the diameter of 1.079mm is output.
Specifically, the laser beam expanding module adopts a plano-concave lens and a plano-convex lens and adopts a Galileo structure.
In particular, the dichroic mirror is an optical plate placed at an angle of 45 ° to the optical axis, through which the trapping beam is redirected, exactly coaxial with the main optical path of the system.
Specifically, the lens group serving as an objective lens includes a first single lens, a second single lens, a third single lens, a first cemented doublet group, a second cemented doublet group, a first cemented triplet group, a second cemented triplet group, and a fourth single lens.
Specifically, the illumination light source is an LED light source, the wavelength is 741nm, and the maximum output power is 170 mW.
Specifically, still include the battery, inside passing through of super portable optical tweezers the battery charges, the battery is equipped with Type-C and charges the mouth, and the mouth that charges is equipped with the visor of stirring formula.
Specifically, super portable optical tweezers include the shell, buffer rubber pad is added to the shell bottom, and the shell top is flip design.
The invention provides an ultra-portable optical tweezers for multi-scene real-time application, which comprises a laser, a laser collimation module, a laser beam expansion module, a dichroic reflector, a lens group serving as an objective lens, a miniature electric sample stage, a condenser lens group, an illumination light source, a photographic eyepiece, a CMOS camera, a control panel and a display screen, wherein the laser is arranged on the laser collimation module; specifically, the device comprises a laser focusing light path and an imaging light path, wherein the laser focusing light path comprises: the laser device emits laser, quasi-parallel light is output through the laser collimation module, the diameter of an emergent beam is increased through the laser beam expansion module, the emergent beam is reflected to a lens group rear pupil serving as an objective lens through the dichroic reflector, and the lens group focuses the laser to the area of the miniature electric sample stage; the imaging light path comprises an illumination link and an imaging link, wherein in the illumination link, an illumination light beam generated by an illumination light source irradiates on a target object in the sample cell through a condensing lens group in a Kohler illumination mode. In the imaging link, the light beam scattered from the target object carries relevant information, and the scattered light beam passes through the sample cell liquid, the cover glass, the lens group, the dichroic mirror and the photographing objective lens and then is imaged on a receiving surface of the CMOS camera. Observe through the display screen, realize controlling the target object in the sample cell under the control of control panel.
As can be seen from the above description of the present invention, compared with the prior art, the present invention has the following advantages:
(1) the invention provides a multi-scene real-time application ultra-portable optical tweezers, which comprises a laser, a laser collimation module, a laser beam expansion module, a dichroic reflector, a lens group serving as an objective lens, a miniature electric sample stage, a condenser lens group, an illumination light source, a photographic eyepiece, a CMOS camera, a control panel and a display screen, wherein the control panel is arranged on the control panel; specifically, a laser of a single wavelength is emitted by a laser; performing laser collimation through a laser collimation module; performing laser beam expansion through a laser beam expansion module; reflecting laser light of a desired wavelength while transmitting light beams of other wavelengths through a dichroic mirror; carrying out laser focusing to a micro electric sample stage through a lens group serving as an objective lens; providing illumination light for the sample cell through an illumination light source and a condenser lens group; acquiring information of the particles through a photographic eyepiece and a CMOS camera; observing the target object in the sample pool through a display screen; the micro electric sample stage is adjusted by the control board to change the sample position until the target object is captured by the optical trap, and then other operations are carried out on the target object. The invention has the characteristics of small volume, integrated structure, convenient carrying and transportation, convenient operation by adopting a customized light path and multi-scene real-time application due to a totally enclosed operating environment, and provides an advanced and portable research tool for scientific research in the field of biological medicine.
(2) The laser collimation module adopted by the invention adopts two vertically placed plano-convex cylindrical lenses, the surface of each cylindrical lens is elliptical, and the semiconductor laser has the characteristic of different divergence angles in two directions vertical to and parallel to the junction plane, so that laser is collimated by the laser collimation module, so that the divergence angles in two directions are reduced, and the quasi-parallel light with the diameter of 1.079mm is output.
(3) The laser beam expanding module adopted by the invention adopts a plano-concave lens and a plano-convex lens to achieve the effect of expanding beams, so that the diameter of an emergent light beam reaches 5.2983mm, and the Galileo structure is adopted, so that the ultra-portability is ensured, and the captured light beam is ensured to fill the rear pupil of the lens group, thereby realizing the strong convergent light field required by the optical tweezers.
(4) The dichroic reflector adopted by the invention is an optical flat plate which is arranged at an angle of 45 degrees with an optical axis, a captured light beam is changed in direction by the dichroic reflector and is strictly coaxial with a main optical path of the system, the captured light beam is emitted into a lens group serving as an objective lens to form a strong convergence gradient light field, and meanwhile, illumination light carrying sample information enters a photographic eyepiece through the dichroic reflector to be imaged for observation.
(5) The lens group used as the objective lens comprises a first single lens, a second single lens, a third single lens, a first double cemented lens group, a second double cemented lens group, a first third cemented lens group, a second third cemented lens group and a fourth single lens; the three-dimensional optical trap satisfies the condition of forming the three-dimensional optical trap and has high magnification at the same time.
(6) The shell adopted by the invention is provided with the buffer shock-absorbing rubber pad at the bottom, so that the shock-absorbing and durable effects are achieved, the top of the shell is designed to be capable of being turned over, and the requirements of touch screen operation and image display portability are met by integrally designing the cover and the touch screen type liquid crystal display screen and selecting the rotating shaft which can rotate 180 degrees at the bottom of the cover.
Drawings
Fig. 1 is a schematic diagram of an ultra-portable optical tweezer for multi-scene real-time application according to an embodiment of the present invention;
FIG. 2 is a schematic view of a micro electric sample stage according to an embodiment of the present invention;
FIG. 3 is a schematic diagram of a laser focusing optical path according to an embodiment of the present invention;
fig. 4 is a dot array diagram of an image plane of a laser focusing optical path according to an embodiment of the present invention.
Fig. 5 is a schematic diagram of an optical path of an imaging link according to an embodiment of the present invention;
fig. 6 is a point array diagram of an image plane of an optical path of an imaging link according to an embodiment of the present invention.
The invention is described in further detail below with reference to the figures and specific examples.
Detailed Description
The invention is further described below by means of specific embodiments.
As shown in fig. 1, the ultra-portable optical tweezers for multi-scene real-time application comprises a laser 1, a laser collimation module 2, a laser beam expanding module 3, a dichroic reflector 4, a lens group serving as an objective lens, a micro electric sample stage 5, a condenser lens group 6, an illumination light source 7, a photographic eyepiece, a CMOS camera 8, a control panel and a display screen; specifically, a laser of a single wavelength is emitted by a laser; performing laser collimation through a laser collimation module; performing laser beam expansion through a laser beam expansion module; reflecting laser light of a desired wavelength while transmitting light beams of other wavelengths through a dichroic mirror; carrying out laser focusing to a micro electric sample stage through a lens group serving as an objective lens; providing illumination light for the sample cell through an illumination light source and a condenser lens group; acquiring information of the particles through a photographic eyepiece and a CMOS camera; observing the target object in the sample pool through a display screen; the micro electric sample stage is adjusted by the control board to change the sample position until the target object is captured by the optical trap, and then other operations are carried out on the target object.
Specifically, the central wavelength of the semiconductor laser is 675nm, the output power is 150mW, and the semiconductor laser is packaged by TO, and the divergence angle θ | | ═ 7.0 ° in the sagittal direction and the divergence angle θ | ═ 15 ° in the meridional direction.
Specifically, the laser collimation module adopts two vertically placed plano-convex cylindrical lenses, the surface type of the cylindrical lenses is an elliptical type, specific data of the module are shown in table 1, and since the semiconductor laser has the characteristic that the divergence angles in two directions perpendicular and parallel to the junction plane are different, laser is collimated through the laser collimation module, so that theta | | ═ 0.1776 °, theta | ═ 0.0745 °, divergence angles in two directions are reduced, and quasi-parallel light with the diameter of 1.079mm is output.
TABLE 1 laser alignment Module concrete data sheet
| Noodle numbering | Radius of curvature | Thickness of | Nd | Vd | Coefficient of cone |
| Article surface | Infinity | 1.5000 | |||
| L1 | Infinity | 3.0000 | 1.5168 | 64.1670 | |
| -3.5235 | 2.0000 | -0.3214 | |||
| L2 | Infinity | 3.0000 | 1.5168 | 64.1670 | |
| -6.6549 | 10.0000 | -0.9688 | |||
| Image plane | Infinity | - |
Specifically, the laser beam expanding module adopts a plano-concave lens and a plano-convex lens to achieve the effect of expanding beams, so that the diameter of an emergent light beam reaches 5.2983mm, a galileo structure is adopted, ultra-portability is ensured, and meanwhile, the fact that the rear pupil of the whole lens group is filled with a captured light beam is guaranteed, so that a strong convergent light field required by the optical tweezers is achieved, and specific data of the module are shown in table 2.
Table 2 laser beam expanding module concrete data table
| Noodle numbering | Radius of curvature | Thickness of | Nd | Vd | Coefficient of cone |
| Article surface | Infinity | Infinity | |||
| Infinity | 5.0000 | ||||
| L1 | -4.1440 | 3.0528 | 1.5168 | 64.1670 | |
| Infinity | 31.9792 | ||||
| L2 | Infinity | 2.7883 | 1.5168 | 64.1670 | |
| -28.0100 | 5.0000 | ||||
| Image plane | Infinity | - |
The dichroic reflector is an optical flat plate which is arranged at an angle of 45 degrees with an optical axis, captured light beams change direction through the dichroic reflector and are strictly coaxial with a main optical path of the system, the captured light beams are emitted into a lens group serving as an objective lens to form a strong convergence gradient light field, and meanwhile, illumination light carrying sample information is transmitted through the dichroic reflector, enters a photographic eyepiece and is imaged for observation.
Specifically, the lens group serving as an objective lens includes a first single lens, a second single lens, a third single lens, a first cemented doublet, a second cemented doublet, a first cemented triplet, a second cemented doublet, and a fourth single lens, and aberrations of the lens group are reduced by adding an aspherical surface. The focal length was 4.9086, the posterior pupil diameter was 5.2983 mm. The three-dimensional optical trap satisfies the condition of forming the three-dimensional optical trap and has high magnification at the same time. Specific data of the lens group are shown in Table 3.
Table 3 detailed data table of lens group serving as objective lens
| Noodle numbering | Radius of curvature | Thickness of | Nd | Vd | Coefficient of cone |
| Article surface | Infinity | Infinity | |||
| L1 | Infinity | 4.7935 | 1.6540 | 57.3 | |
| -2.6097 | 0.9168 | ||||
| L2 | -11.4608 | 5.8158 | 1.5170 | 52.4 | |
| -8.7568 | 0.9440 | ||||
| L3 | -14.6836 | 5.9859 | 1.4980 | 82.5 | |
| -10.8227 | 1.2966 | ||||
| L4 | -27.7722 | 5.9200 | 1.5570 | 50.2 | |
| -21.1147 | 6.5152 | ||||
| L6 | 249.8517 | 9.1532 | 1.6230 | 58.7 | |
| L7 | 16.7569 | 4.4786 | 1.4620 | 78.0 | |
| -26.6490 | 13.7688 | ||||
| L8 | 43.7116 | 7.4423 | 1.6140 | 60.5 | |
| L9 | 12.4679 | 6.8209 | 1.4620 | 78.0 | |
| L10 | -19.9275 | 7.5512 | 1.4500 | 67.4 | |
| 1991.7624 | 1.8364 | ||||
| L11 | 15.4060 | 9.9720 | 1.5170 | 52.4 | |
| L12 | -10.6577 | 6.2170 | 1.4500 | 67.4 | |
| L13 | 16.3821 | 8.4368 | 1.8160 | 46.6 | |
| 5.4743 | 1.9531 | 0.0742 | |||
| L14 | -4.8671 | 8.6967 | 1.8160 | 46.6 | |
| -13.5626 | 5.3092 | 2.5766E-04 | |||
| Image plane | Infinity | - |
The miniature electric sample stage is controlled by the single chip microcomputer, and can move in small size in the x, y and z directions, so that accurate operation is realized, and inaccuracy of manual adjustment is avoided. The sample cell is fixed in miniature electronic sample bench to adopt plug-type structure to carry out the sample and place, seal the processing, repeatedly usable keeps test sample's maneuverability for a long time simultaneously, can disinfect to this closed sample cell through modes such as adopting ultraviolet lamp to shine. Fig. 2 is a schematic view of a micro electric sample stage.
Fig. 3 is a schematic diagram of a laser focusing light path, in which a laser is emitted from a laser, quasi-parallel light is output by a laser collimating module, an emergent beam diameter is increased by a laser beam expanding module, the emergent beam is reflected by a dichroic mirror to a rear pupil of a lens group serving as an objective lens, and the lens group with a high numerical aperture focuses the laser to a micro electric sample stage area.
Fig. 4 is a dot array diagram of the laser focusing optical path image plane.
The illuminating light source is an LED light source, the wavelength of the LED light source is 741nm, and the maximum output power is 170 mW. And an illumination light beam generated by the illumination light source is irradiated on the target object in the sample cell through the condenser lens group in a Kohler illumination mode. Specific data of the condenser lens group are shown in table 4.
TABLE 4 detailed data sheet of condenser lens group
| Noodle numbering | Radius of curvature | Thickness of | Nd | Vd | Coefficient of cone |
| Article surface | Infinity | 15.4205 | |||
| -33.3498 | 1.0000 | ||||
| L1 | 3.3565 | 4.0000 | 1.5168 | 64.1670 | |
| 7.2159 | 3.5743 | 22.9342 | |||
| L2 | -82.1654 | 4.0000 | 1.5168 | 64.1670 | |
| 4.0000 | 6.0000 | -95.9003 | |||
| Image plane | Infinity | - |
The scattered light beam of the target from the sample cell passes through the sample cell liquid, the cover glass, the lens group and the dichroic mirror, enters the photographic eye lens, and then is imaged on the detector target surface of the CMOS camera. The details of the eyepiece are shown in table 5.
TABLE 5 detailed data sheet of eyepiece
| Noodle numbering | Radius of curvature | Thickness of | Nd | Vd | Coefficient of cone |
| Article surface | Infinity | Infinity | |||
| L1 | -44.0150 | 7.0926 | 1.5596 | 61.2 | |
| -6.3784 | 0.8646 | ||||
| L2 | 8.6129 | 3.3427 | 1.5596 | 61.2 | |
| 15.9869 | 1.0499 | ||||
| L3 | -3.6391 | 3.3443 | 1.5596 | 61.2 | |
| L4 | -2.3551 | 3.6167 | 1.6889 | 31.1 | |
| -3.2062 | 1.7309 | ||||
| L5 | 1.3270 | 3.5331 | 1.6471 | 33.9 | |
| Image plane | Infinity | - |
The imaging light path comprises an illumination link and an imaging link, wherein in the illumination link, an illumination light beam generated by an illumination light source irradiates on a target object in the sample cell through a condensing lens group in a Kohler illumination mode.
Fig. 5 is a schematic diagram of an optical path of an imaging segment, wherein the imaging segment is that a light beam scattered from a target object carries relevant information, and the scattered light beam is imaged on a receiving surface of a CMOS camera after passing through a sample cell liquid, a cover glass, a lens group, a dichroic mirror and a photographing objective lens. Observe through the display screen, realize controlling the target object in the sample cell under the control of control panel.
Fig. 6 is a dot array diagram of the image plane of the optical path of the imaging element.
The traditional optical tweezers industrial grade CMOS camera is connected with a PC (personal computer) end and displays images, a single chip microcomputer is used for controlling screen display, a circuit is designed to enable the CMOS camera to display images on a liquid crystal display screen, and image data are transmitted to a control panel through a USB (universal serial bus) interface of the CMOS camera.
The inside of the ultra-portable optical tweezers for multi-scene real-time application provided by the embodiment of the invention is powered by the battery, and the battery is provided with the Type-C charging port, so that the external power supply can be timely carried out through the charging port when needed. The charging port is provided with a toggle protective cover which can be protected when the charging port is not used.
According to the ultra-portable optical tweezers for the multi-scene real-time application, the shell is made of the polypropylene composite material, and the ultra-portable optical tweezers have the characteristics of high strength, high toughness, bearing, pressure resistance and wear resistance. The bottom of the box is added with a buffer and shock-absorbing rubber pad, so that the box is shock-absorbing and durable. The top of the shell is designed to be capable of being turned over, and the touch screen operation and the image display portable requirement are realized through the integrated design of the cover and the touch screen type liquid crystal display screen and the selection of the rotating shaft which can rotate 180 degrees at the bottom of the cover. The rubber handle is horizontally arranged at the center of the top of the shell, and the lower surface of the rubber handle is in a waveform matched with the shape of the finger belly, so that the rubber handle is in accordance with human engineering, labor-saving and durable.
The invention provides a multi-scene real-time application ultra-portable optical tweezers, which comprises a laser, a laser collimation module, a laser beam expansion module, a dichroic reflector, a lens group serving as an objective lens, a miniature electric sample stage, a condenser lens group, an illumination light source, a photographic eyepiece, a CMOS camera, a control panel and a display screen, wherein the control panel is arranged on the control panel; specifically, a laser of a single wavelength is emitted by a laser; performing laser collimation through a laser collimation module; performing laser beam expansion through a laser beam expansion module; reflecting laser light of a desired wavelength while transmitting light beams of other wavelengths through a dichroic mirror; carrying out laser focusing to a micro electric sample stage through a lens group serving as an objective lens; providing illumination light for the sample cell through an illumination light source and a condenser lens group; acquiring information of the particles through a photographic eyepiece and a CMOS camera; observing the target object in the sample pool through a display screen; the micro electric sample stage is adjusted by the control board to change the sample position until the target object is captured by the optical trap, and then other operations are carried out on the target object. The utility model has the characteristics of small, structure integration, portable and transportation, adopt the customization light path be convenient for operate, because thereby totally enclosed operational environment can multi-scene use in real time, provide advanced portable research tool for the scientific research of biomedical field.
The laser collimation module adopts two vertically-arranged plano-convex cylindrical lenses, the surface types of the cylindrical lenses are elliptical, specific data of the module are shown in table 1, and the semiconductor laser has the characteristic that divergence angles in two directions vertical to and parallel to a junction plane are different, so that laser is collimated by the laser collimation module, so that theta | | ═ 0.1776 degrees and theta | -, 0.0745 degrees are formed, the divergence angles in the two directions are reduced, and quasi-parallel light with the diameter of 1.079mm is output.
The laser beam expanding module adopted by the invention adopts a plano-concave lens and a plano-convex lens to achieve the effect of expanding beams, so that the diameter of an emergent light beam reaches 5.608mm, and the Galileo structure is adopted, so that the ultra-portability is ensured, and the captured light beam is ensured to fill the whole rear pupil of the lens group serving as an objective lens, thereby realizing the strong convergent light field required by the optical tweezers.
The dichroic reflector adopted by the invention is an optical flat plate which is arranged at an angle of 45 degrees with an optical axis, a captured light beam is changed in direction by the dichroic reflector and is strictly coaxial with a main optical path of the system, the captured light beam is emitted into a lens group serving as an objective lens to form a strong convergence gradient light field, and meanwhile, illumination light carrying sample information enters a photographic eyepiece through the dichroic reflector to be imaged for observation.
The lens group used as the objective lens comprises a first single lens, a second single lens, a third single lens, a first double cemented lens group, a second double cemented lens group, a first third cemented lens group, a second third cemented lens group and a fourth single lens; the three-dimensional optical trap satisfies the condition of forming the three-dimensional optical trap and has high magnification at the same time.
The shell adopted by the invention is provided with the buffer shock-absorbing rubber pad at the bottom, so that the shock-absorbing and durable effects are achieved, the top of the shell is designed to be capable of being turned over, and the requirements of touch screen operation and image display portability are met by integrally designing the cover and the touch screen type liquid crystal display screen and selecting the rotating shaft which can rotate 180 degrees at the bottom of the cover.
The above description is only an embodiment of the present invention, but the design concept of the present invention is not limited thereto, and any insubstantial modifications made by using the design concept should fall within the scope of infringing the present invention.
Claims (10)
1. The ultra-portable optical tweezers for multi-scene real-time application is characterized by comprising a laser, a laser collimation module, a laser beam expansion module, a dichroic reflector, a lens group serving as an objective lens, a miniature electric sample stage, a condenser lens group, an illumination light source, a photographic eyepiece, a CMOS camera, a control panel and a display screen; specifically, a laser of a single wavelength is emitted by a laser; performing laser collimation through a laser collimation module; performing laser beam expansion through a laser beam expansion module; reflecting laser light of a desired wavelength while transmitting light beams of other wavelengths through a dichroic mirror; carrying out laser focusing to a micro electric sample stage through a lens group serving as an objective lens; providing illumination light for the sample cell through an illumination light source and a condenser lens group; acquiring information of the particles through a photographic eyepiece and a CMOS camera; observing the target object in the sample pool through a display screen; the micro-electric sample stage is adjusted by the control board to change the sample position until the target object is captured by the optical trap and then operated.
2. The ultra-portable optical tweezers of claim 1, wherein the semiconductor laser has a center wavelength of 675nm, an output power of 150mW, and a TO package, and the divergence angle θ | | | -7.0 ° in a sagittal direction and the divergence angle θ | -15 ° in a meridional direction.
3. The ultra-portable optical tweezers of claim 1, wherein the laser collimating module is formed by two vertically disposed plano-convex cylindrical lenses, the cylindrical lens surface is elliptical, and the laser collimating module collimates the laser light such that the divergence angle θ | | | -0.1776 ° in the sagittal direction, the divergence angle θ | -0.0745 ° in the meridional direction, and the quasi-parallel light with a diameter of 1.079mm is output.
4. The ultra-portable optical tweezers of claim 1, wherein the laser beam expanding module is implemented by using a plano-concave lens and a plano-convex lens, and a galileo structure.
5. The ultra-portable optical tweezers of claim 1, wherein the dichroic mirror is an optical plate placed at an angle of 45 ° with respect to the optical axis, and the trapping beam is redirected by the dichroic mirror to be exactly coaxial with the main optical path of the system.
6. The ultra-portable optical tweezers of claim 1, wherein the lens group acting as an objective lens comprises a first single lens, a second single lens, a third single lens, a first double cemented lens group, a second double cemented lens group, a first triple cemented lens group, a second triple cemented lens group, and a fourth single lens.
7. The ultra-portable optical tweezers of claim 1, wherein the illumination light source is an LED light source, the wavelength is 741nm, and the maximum output power is 170 mW.
8. The ultra-portable optical tweezers for multi-scene real-time application according to claim 1, further comprising a battery, wherein the inside of the ultra-portable optical tweezers is charged through the battery, the battery is provided with a Type-C charging port, and the charging port is provided with a toggle protective cover.
9. The ultra-portable optical tweezers for multi-scene real-time application according to claim 1, wherein the ultra-portable optical tweezers comprise a housing, a cushion rubber pad is added to the bottom of the housing, and the top of the housing is designed to be a flip cover.
10. The ultra-portable optical tweezers for multi-scene real-time application is characterized by comprising a laser, a laser collimation module, a laser beam expansion module, a dichroic reflector, a lens group serving as an objective lens, a miniature electric sample stage, a condenser lens group, an illumination light source, a photographic eyepiece, a CMOS camera, a control panel and a display screen; specifically, the device comprises a laser focusing light path and an imaging light path, wherein the laser focusing light path comprises: the laser device emits laser, quasi-parallel light is output through the laser collimation module, the diameter of an emergent beam is increased through the laser beam expansion module, the emergent beam is reflected to a lens group rear pupil serving as an objective lens through the dichroic reflector, and the lens group focuses the laser to the area of the miniature electric sample stage; the imaging light path comprises an illumination link and an imaging link, wherein in the illumination link, an illumination light beam generated by an illumination light source is irradiated on a target object in the sample cell through a condensing lens group in a Kohler illumination mode; in the imaging link, light beams scattered from a target object carry information, and the scattered light beams are imaged on a receiving surface of a CMOS camera after passing through sample pool liquid, a cover glass, a lens group, a dichroic reflector and a photographing objective lens; observe through the display screen, realize controlling the target object in the sample cell under the control of control panel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110372705.6A CN113156634A (en) | 2021-04-07 | 2021-04-07 | Multi-scene real-time application ultra-portable optical tweezers |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110372705.6A CN113156634A (en) | 2021-04-07 | 2021-04-07 | Multi-scene real-time application ultra-portable optical tweezers |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113156634A true CN113156634A (en) | 2021-07-23 |
Family
ID=76888584
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110372705.6A Pending CN113156634A (en) | 2021-04-07 | 2021-04-07 | Multi-scene real-time application ultra-portable optical tweezers |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113156634A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023214197A1 (en) * | 2022-05-02 | 2023-11-09 | 刘正锋 | Method and device for increasing resolving power of optical instrument by applying new theory of optics |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108445641A (en) * | 2018-05-11 | 2018-08-24 | 长春理工大学 | A kind of tunable semiconductor laser optical optical tweezers system |
| US20180246308A1 (en) * | 2015-09-23 | 2018-08-30 | Peking University | STED super-resolution microscope and adjusting method based on a first-order Bessel beam |
| CN110082282A (en) * | 2019-04-18 | 2019-08-02 | 天津大学 | The method and apparatus for realizing optical ultra-discrimination imaging based on optical tweezer |
| CN215953957U (en) * | 2021-04-07 | 2022-03-04 | 华侨大学 | Multi-scene ultra-portable optical tweezers device |
-
2021
- 2021-04-07 CN CN202110372705.6A patent/CN113156634A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20180246308A1 (en) * | 2015-09-23 | 2018-08-30 | Peking University | STED super-resolution microscope and adjusting method based on a first-order Bessel beam |
| CN108445641A (en) * | 2018-05-11 | 2018-08-24 | 长春理工大学 | A kind of tunable semiconductor laser optical optical tweezers system |
| CN110082282A (en) * | 2019-04-18 | 2019-08-02 | 天津大学 | The method and apparatus for realizing optical ultra-discrimination imaging based on optical tweezer |
| CN215953957U (en) * | 2021-04-07 | 2022-03-04 | 华侨大学 | Multi-scene ultra-portable optical tweezers device |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023214197A1 (en) * | 2022-05-02 | 2023-11-09 | 刘正锋 | Method and device for increasing resolving power of optical instrument by applying new theory of optics |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11609416B2 (en) | Imaging systems with micro optical element arrays and methods of specimen imaging | |
| JP6466930B2 (en) | Equipment for light sheet microscopy | |
| US20170131534A1 (en) | Microscope | |
| US20210239961A1 (en) | Method for generating an overview image using a large aperture objective | |
| TW201142352A (en) | Fluorescence micro imaging system | |
| US20110273767A1 (en) | Movable Objective Lens Assembly For an Optical Microscope and Optical Microscopes Having Such an Assembly | |
| US7288751B2 (en) | Imaging system, methodology, and applications employing reciprocal space optical design | |
| CN104287692B (en) | A kind of eye-ground photography device | |
| CN215953957U (en) | Multi-scene ultra-portable optical tweezers device | |
| JP2021514731A (en) | Mobile communication device-based corneal shape analysis system | |
| CN113156634A (en) | Multi-scene real-time application ultra-portable optical tweezers | |
| WO2018087665A1 (en) | Portable upright bright field microscope with smart device compatibility | |
| KR102625431B1 (en) | Dynamic focus and zoom system for use with widefield, confocal, and multiphoton microscopes | |
| CN205263394U (en) | But device is picked up something with tweezers to automatically move's holographic light | |
| CN103676122A (en) | Small fluorescent/bright field optical imaging system, optical imaging method and purpose thereof | |
| US20160048009A1 (en) | Microscope apparatus and applications thereof | |
| US10466461B2 (en) | Dynamic focus and zoom system for use with wide-field, confocal and multiphoton microscopes | |
| WO2020153996A1 (en) | Dynamic focus and zoom system for use wide-field, confocal and multiphoton microscopes | |
| JP7207860B2 (en) | Sample observation device | |
| US12001003B2 (en) | Immersion front-end lens system | |
| KR102719840B1 (en) | Dynamic focus and zoom system for use with widefield, confocal, and multiphoton microscopes | |
| Ulanowski et al. | Compact optical trapping microscope using a diode laser | |
| Shan et al. | Controlled rotation of cells using a single-beam anisotropic optical trap | |
| CN219915381U (en) | Small microscopic observation device for bright field fluorescence | |
| CN111344620B (en) | Dynamic focusing and zooming system for wide-field, confocal and multiphoton microscopes |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |