CN113151101A - Serratia marcescens and application thereof - Google Patents
Serratia marcescens and application thereof Download PDFInfo
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- CN113151101A CN113151101A CN202110521036.4A CN202110521036A CN113151101A CN 113151101 A CN113151101 A CN 113151101A CN 202110521036 A CN202110521036 A CN 202110521036A CN 113151101 A CN113151101 A CN 113151101A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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Abstract
The invention discloses serratia marcescens and application thereof, and belongs to the technical field of agricultural microorganisms. The preservation number of the serratia marcescens is as follows: CGMCC No.21857, the preservation time is as follows: 2021, 3, 9 months, the preservation address is: the institute of microbiology, national academy of sciences No. 3, Xilu No. 1, Beijing, Chaoyang, Beijing. The serratia marcescens is separated from the sick charomiasis japonica longicorn larvae, and the living bodies of the serratia marcescens can be used as biological agents or bacterial fertilizers prepared by taking the serratia marcescens living bodies or metabolites thereof as active ingredients, so that the purpose of preventing and treating the charomiasis japonica is realized, and the yield and the quality of coffee can be improved.
Description
Technical Field
The invention relates to the technical field of agricultural microorganisms, and relates to serratia marcescens and application thereof.
Background
Yunnan province is the main production area of the arabica coffee in China, the biggest arabica coffee planting base and trade export gathering place in China, and the planting areas are mainly distributed in 42 counties (cities) of 11 states (cities) and dry-hot valley areas. The yield of Yunnan arabica is 15.84 ten thousand tons in 2016, the Yunnan arabica accounts for 98.8 percent of the whole country, and the planting area reaches 116973.33hm2Accounting for 98.44% of the country, has become a major source of income for local farmers, a major tax source for local finances, and an important agricultural product for earnings for exports. With the single large-area planting of the coffea arabica, a stable ecological system is formed for coffee pests, the fallen leaves of the coffee trees create favorable conditions for the harm of the coffee pests, the management level is reduced, and the harm of the pests is heavier and heavier with the increase of the age of the coffee trees. More pests harmful to coffee are mainly coleopteran pests, such as coffee beetle and longhorn beetle. The existing prevention and control methods mainly comprise the steps of cleaning damaged branches, planting shaded trees, hanging trap trees, chemical prevention and control and the like, but the prevention and control methods have poor effects.
In recent years, microorganisms have been a very important place in modern agricultural production and scientific research as a basis for preparing efficient, safe and residue-free biopesticides. However, few studies on the prevention and control of coleopteran pests, particularly coffee deinsectization longicorn beetles, have been reported at home and abroad so far.
Disclosure of Invention
The invention aims to provide serratia marcescens and application thereof, the serratia marcescens can obviously inhibit the larvae of the coffee tiger beetles and has good effect and low cost; the bacterium or the metabolite thereof is used as an active ingredient to prepare a biological agent or a bacterial fertilizer, and has important significance for the prevention and control of the coffee deinsectization carinii.
In order to achieve the purpose, the invention provides the following scheme:
the invention provides Serratia marcescens Serratia marcescens with a preservation number of: CGMCC No.21857, the preservation time is as follows: 2021, 3/9, the preservation unit is: china general microbiological culture Collection center; the preservation address is as follows: the institute of microbiology, national academy of sciences No. 3, Xilu No. 1, Beijing, Chaoyang, Beijing.
The invention also provides application of the serratia marcescens in preparing a biological preparation for preventing and treating coffee and deina nivalis.
The invention also provides application of the serratia marcescens in preparing bacterial manure for preventing and treating coffee and tiger beetles.
The invention also provides a preparation method of the fermentation liquor of the serratia marcescens, which comprises the following steps:
inoculating Serratia marcescens in a YSP liquid culture medium, and culturing at 20-28 deg.C and pH of 6-8 for 12-16h to obtain seed liquid;
inoculating the obtained seed liquid into a YSP liquid culture medium according to the inoculation amount of 0.5-2%, and culturing at 20-28 deg.C and pH of 6-8 for 38-84h to obtain serratia marcescens fermentation liquid.
Preferably, the YSP liquid medium comprises the following components: 3-10g of yeast extract powder, 8-15g of peptone, 15-25g of sucrose and 1000mL of water.
The invention also provides the serratia marcescens fermentation liquid obtained by the preparation method.
The invention also provides application of the serratia marcescens fermentation liquid in preparing a biological preparation for preventing and treating coffee and black-striped tiger longhorn beetles.
The invention also provides application of the serratia marcescens fermentation liquid in preparing bacterial manure for preventing and treating coffee and tiger beetles.
The invention discloses the following technical effects:
the serratia marcescens disclosed by the invention is separated from diseased coffee cherokee anoplophora longicorn larvae, and a biological preparation is prepared by taking a serratia marcescens living body or a metabolite thereof as an active ingredient, is applied to preventing and treating coffee cherokee anoplophora longicorn, and has an inhibition rate of 76.67%; meanwhile, the compound can be used as an active ingredient for preparing biological bacterial manure, can prevent and treat coffee and deinocarpus alternatus, and can improve the yield and quality of coffee.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without inventive exercise.
FIG. 1 shows the effect of different media on the growth of Serratia marcescens ND 1-1;
FIG. 2 shows the effect of different carbon sources on the growth rate of Serratia marcescens ND 1-1;
FIG. 3 shows the effect of different nitrogen sources on the growth of Serratia marcescens ND 1-1;
FIG. 4 is a graph showing the effect of pH on the growth rate of Serratia marcescens ND 1-1;
FIG. 5 is a graph showing the effect of the culture temperature on the growth rate of Serratia marcescens ND 1-1;
FIG. 6 is a graph showing the effect of inoculum size on the growth rate of Serratia marcescens ND 1-1;
FIG. 7 is a graph showing the effect of the culture time on the growth rate of Serratia marcescens ND 1-1;
FIG. 8 shows the phylogenetic tree of Serratia marcescens ND 1-1.
Detailed Description
The present invention will now be described in detail by way of examples, which should not be construed as limiting the invention but as providing more detailed descriptions of certain aspects, features and embodiments of the invention.
The test methods used in the following examples are all conventional methods unless otherwise specified; the materials, reagents and the like used are, unless otherwise specified, commercially available reagents and materials.
Example 1 isolation, purification and characterization of Serratia marcescens
Firstly, separating and purifying strains
Separated from diseased coffee caribous of caribous sinica which are collected in a Nandao river village coffee plantation base in the region of Cistus arundinacea in Puer city, and relevant microbial pesticides have never been used in the plantation base. The following steps are adopted for separation and purification:
1. first, the body surface of the caribou carinii larva was rinsed 3 times with 75% alcohol for 2 minutes each, rinsed 3 times with sterile water, and ground.
2. And (3) sterilization: the experimental devices such as the culture dish, the centrifuge tube, the test tube, the gun head and the like and the sterile water are sterilized by high pressure for 30 minutes at the temperature of 121 ℃ under the pressure of 0.1 MPa.
3. Grinding: the sick coffee Kalopanax strigae nakai is sterilized and then put into a sterile centrifuge tube, 1mL of sterile water is added, and the mixture is fully ground by a grinding rod.
4. Gradient dilution: taking 1mL of the ground juice, and putting the ground juice into a test tube containing 9mL of sterile water to obtain 10-1Diluted bacterial liquid, from 10-1Taking 1mL of the diluted bacteria liquid and putting the diluted bacteria liquid into a test tube containing 9mL of sterile water to obtain 10-2Diluting the bacterial liquid, and repeating the steps to obtain 10-3、10-4、10-5、10-6、10-7And (4) diluted bacteria liquid.
5. Preparation of culture Medium
Medium for isolation: beef extract peptone medium (NA medium) comprising: 3g of beef extract, 10g of peptone, 5g of NaCl, 18g of agar and 1000mL of water, wherein the pH value is 7.0-7.2, and the beef extract is sterilized at 121 ℃ for 20 min.
Culture medium for purification: an LB medium comprising: 10g of peptone; 5g of yeast extract; 10g of sodium chloride; 15g of agar; 1000mL of distilled water; sterilizing at 121 deg.C for 20min and pH 7.0.
Pour plate, pour about 15mL of medium per dish, lay flat and stand, wait to cool for use.
6. Coating a flat plate: diluting the bacterial liquid 10-4、10-5、10-6、10-7The plates were coated separately, 3 dishes per gradient, and the bottom of the dish was marked.
7. Culturing: the plates were inverted and incubated in an incubator at 28 ℃ for 3-5 days.
8. And (3) purifying bacteria: under the aseptic condition, a single colony growing on the NA culture medium is picked on an LB culture medium by an inoculating loop to be subjected to streak culture; the single colony obtained after 3-4 purifications was named ND 1-1.
II, identifying strains
(1) Identification of the culture characteristics of the strains
The separated strain is coated on an LB solid culture medium, and is cultured for 72h under the conditions of 24 ℃ and pH value of 7, and the colony characteristics of the strain are observed.
As a result, it was found that: the colony diameter is 1-3mm, red, round, surface convex, moist, glossy, and the edge of the long rod of the corrugated bacteria is 2.22-2.86 μm.
(2) Identification of physiological and biochemical indicators
The strain obtained by the above isolation was subjected to physiological and biochemical identification tests according to the identification method of Serratia bacteria in a common bacteria system identification manual (Toxiu bead et al, common bacteria system identification manual [ M ]. Beijing: scientific Press, 2001.).
The identification result shows that: the bacterium is gram negative, the bacterium starch hydrolysis test is negative, the grease hydrolysis test is negative, and the litmus milk test is positive; the fermented glucose can produce both acid and gas; indole test is negative, methyl red test is positive, volt-common test is positive, gelatin test is positive, and hydrogen sulfide test is positive.
(3) Sequence analysis of strains
Selecting 1 single colony of the separated strain, inoculating to YSP liquid culture medium, culturing at 24 deg.C and pH of 7 for 16h, collecting bacterial liquid, centrifuging at 15000rpm for 15min, and collecting thallus. According to the extraction method of the bacterial DNA extraction kit of Tiangen (Beijing) Biotechnology limited company, the bacterial genome DNA is extracted and PCR amplification is carried out by using a general bacterial specific primer. The amplified product is subjected to electrophoresis detection, a specific fragment appears at 1500bp, the sequence of the fragment is determined, and the result shows that the 16S rRNA sequence (SEQ ID NO: 1) of the obtained strain is determined to be
GATGCGCGGCTACCATGCAGTCGAGCGGTAGCACAGGGGAGCTTGCTCCCTGGG TGACGAGCGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGA TAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGG GACCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGG GGTAATGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCA CACTGGAACTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATAT TGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTGTGTGAAGAAGGCCTT CGGGTTGTAAAGCACTTTCAGCGAGGAGGAAGGTGGTGAACTTAATACGTTCATC AATTGACGTTACTCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGG TAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAGG CGGTTTGTTAAGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCATTTGA AACTGGCAAGCTAGAGTCTCGTAGAGGGGGGTAGAATTCCAGGTGTAGCGGTGA AATGCGTAGAGATCTGGAGGAATACCGGTGGCGAAGGCGGCCCCCTGGACGAAG ACTGACGCTCAGGTGCGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTA GTCCACGCTGTAAACGATGTCGATTTGGAGGTTGTGCCCTTGAGGCGTGGCTTCC GGAGCTAACGCGTTAAATCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACT CAAATGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTG
Sequence alignment analysis was then performed by BLAST software and a strain evolutionary tree was constructed (see FIG. 8).
Through the culture characteristics, physiological and biochemical characteristics and 16S rDNA analysis, the strain obtained by the separation is identified to be Serratia marcescens Serratia marcocens.
Example 2 screening of fermentation culture conditions for Serratia marcescens
Selecting 1 Serratia marcescens ND1-1 single colony, inoculating to YSP liquid culture medium, culturing at 24 deg.C under pH of 7 for 16h to obtain seed liquid.
Inoculating the obtained seed solution on a YSP culture medium, and culturing at 24 deg.C and pH of 7 to obtain Serratia marcescens ND1-1 fermentation liquid for optimizing the following fermentation culture conditions.
1. The culture medium is preferably
The strain is cultured by adopting five culture media including YSP, NYB, NA, LB and CM, and the growth condition of Serratia marcescens ND1-1 is observed. Wherein, the specific culture medium comprises the following components:
YSP medium: 5g of yeast extract powder, 10g of peptone, 20g of sucrose and 1000mL of water; sterilizing at 121 deg.C for 20 min.
NYBD medium: 5g of yeast extract powder, 8g of beef extract, 10g of glucose and 1000mL of water; sterilizing at 121 deg.C for 20 min.
NA medium: 3g of beef extract, 10g of peptone, 5g of NaCl, 18g of agar and 1000mL of water, and sterilizing at 121 ℃ for 20 min.
LB culture medium: 10g of peptone, 5g of yeast extract, 10g of sodium chloride, 15g of agar and 1000mL of distilled water, and sterilizing at 121 ℃ for 20 min.
CM medium: (NH)4)2SO4 2g，MgSO4·7H2O 0.2g，K2HPO4 4g，KH2PO46g, 5g of glucose, 1g of sodium citrate, 15g of agar and 1000mL of water; sterilizing at 121 deg.C for 20 min.
As shown in FIG. 1, Serratia marcescens ND1-1 grows on YSP medium at the fastest rate with OD value of 2.7909, followed by NYB medium with OD value of 2.7094.
2. Effect of different carbon sources on growth Rate of the Strain
The strains are cultured by adopting five carbon sources such as maltose, lactose, glucose, sucrose, starch and the like, and the growth condition of the serratia marcescens ND1-1 is observed.
As shown in FIG. 2, the most suitable carbon source for Serratia marcescens ND1-1 was sucrose, and the OD value of the bacterial liquid was 2.7909.
3. Effect of different Nitrogen sources on growth of the Strain
The bacterial strain is cultured by five nitrogen sources of ammonium chloride, yeast powder, ammonium nitrate, serine and peptone, and the growth condition of Serratia marcescens ND1-1 is observed.
As can be seen from FIG. 3, Serratia marcescens ND1-1 grows most rapidly on the yeast extract powder, and the OD value is 2.8930.
4. Effect of pH on growth Rate of Strain
The strain was cultured at pH 7, pH 4, pH 5, pH 6, pH 7, pH 8, pH 9, pH 10, and the growth of serratia marcescens ND1-1 was observed under different pH environments.
As can be seen from FIG. 4, the optimum growth pH for Serratia marcescens ND1-1 is 7.
5. Effect of temperature on growth of the Strain
The strain culture temperature is set to 6 treatments, namely 16 ℃, 20 ℃, 24 ℃, 28 ℃, 32 ℃, 36 ℃ and 40 ℃ respectively, and the growth condition of the serratia marcescens ND1-1 at different temperatures is observed.
As shown in FIG. 5, Serratia marcescens ND1-1 has an optimum growth temperature of 24 deg.C
6. Effect of inoculum size on growth of strains
Inoculating with 0.05%, 0.1%, 0.5%, 1%, 2%, 2.5%, 3% inoculum size respectively, and observing the growth of Serratia marcescens ND1-1 under different inoculum sizes.
As can be seen from FIG. 6, the bacteria of ND1-1 grew most rapidly when the inoculation amount was 1%.
7. Effect of incubation time on Strain growth
The culture time of the strains is respectively set to be 2h, 4h, 6h, 8h, 10h, 12h, 14h, 16h, 18h, 20h, 22h, 24h, 36h, 38h, 48h, 60h, 72h, 84h, 96h and 108h, and the growth conditions of the strains are observed at different culture times.
As shown in FIG. 7, the amount of Serratia marcescens ND1-1 growth increased with the increase of the culture time, the growth rate reached the maximum value at 72h, and the shaking culture was continued for a prolonged period of time, whereby the growth rate of the bacteria began to slow down and the OD value decreased.
According to the screening and optimization of the conditions, the optimal culture medium of the serratia marcescens ND1-1 separated by the invention is a YSP culture medium which comprises the following components: 5g of yeast extract powder, 10g of peptone, 20g of sucrose and 1000mL of water.
The optimal fermentation culture conditions are as follows: inoculating Serratia marcescens bacterial liquid into YSP culture medium according to 1% inoculation amount, culturing at 24 deg.C and initial pH of 7 for 72 hr to obtain Serratia marcescens fermentation liquid with bacterial content of 1.5 × 109-3×109。
Example 3 measurement of insecticidal Effect of Serratia marcescens ND1-1 fermentation liquid on coffee beetle larvae
And (3) obtaining the serratia marcescens ND1-1 fermentation liquor according to the screened optimal fermentation culture conditions, and determining the insecticidal action of the serratia marcescens ND1-1 fermentation liquor on the larvae of the coffee-killed carinii. The specific determination method comprises the following steps: soaking 10 healthy coffee deinsectization carinii larvae with consistent size in the bacterial fermentation broth for 3s, independently placing into a finger-shaped tube, sealing with absorbent cotton, repeating for 3 times, soaking in sterilized water for 3s for comparison, and observing and recording every day at regular time. Finally, the corrected cumulative mortality is calculated.
Indoor toxicity test results show that the corrected cumulative mortality rate of the serratia marcescens ND1-1 to the larvae of the coffee-killed caridina domestica (Xylotrechus quadrupipes Chevrola) reaches 76.67% (see Table 1).
TABLE 1 toxicity of ND1-1 to Caffee-exterminating Chonemus teres larvae
Example 4 preparation of bacterial manure from Serratia marcescens ND1-1
According to the screened optimal fermentation culture conditions, the serratia marcescens ND1-1 is inoculated to a YSP culture medium for culture to obtain serratia marcescens fermentation liquor, then the obtained fermentation liquor is added into an organic fertilizer according to 3.5 percent of the weight of the organic fertilizer, and the organic fertilizer and the fermentation liquor are uniformly mixed to prepare the serratia marcescens fertilizer. The biological bacterial fertilizer has viable count greater than 0.58 × 109cfu/g。
Example 5 application of bacterial manure to increase the production of Coffea arabica
The method comprises the steps of selecting the small-particle coffee trees which normally bear fruits in 5 years in a small-particle coffee planting area in Lincang city of Yunnan province for experiment, selecting 50 trees, dividing the trees into two groups randomly, applying equal amount of organic fertilizer to each small-particle coffee tree in the A group and the B group in a hole mode at the root base part, applying the serratia marcescens fertilizer prepared in the embodiment 5 to the B group, and enabling other planting and management conditions to be completely the same.
After harvesting the arabica seeds, the average yield of the group B was found to be 20.35% higher than the average yield of the group A; in addition, in the management process, the obvious harm reduction of coffee-killed carinii to B group arabica of the serratia marcescens fertilizer prepared by the invention is found, the work of preventing and controlling the harm of coffee-killed carinii is greatly reduced, and the yield and the quality of the arabica are improved.
The above-described embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solutions of the present invention can be made by those skilled in the art without departing from the spirit of the present invention, and the technical solutions of the present invention are within the scope of the present invention defined by the claims.
Claims (8)
1. The serratia marcescens strain is characterized by being deposited as follows: CGMCC No.21857, the preservation time is as follows: 2021, 3, 9 months, the preservation address is: the institute of microbiology, national academy of sciences No. 3, Xilu No. 1, Beijing, Chaoyang, Beijing.
2. Use of serratia marcescens according to claim 1 for the preparation of a biological preparation for controlling coffee-killed carinii.
3. The use of serratia marcescens according to claim 1 in the preparation of a bacterial manure for controlling coffee and tiger beetles.
4. A method for preparing a fermentation broth of Serratia marcescens according to claim 1, comprising the steps of:
inoculating Serratia marcescens in a YSP liquid culture medium, and culturing at 20-28 deg.C and pH of 6-8 for 12-16h to obtain seed liquid;
inoculating the obtained seed liquid into a YSP liquid culture medium according to the inoculation amount of 0.5-2%, and culturing at 20-28 deg.C and pH of 6-8 for 38-84h to obtain serratia marcescens fermentation liquid.
5. The method of claim 4, wherein the YSP liquid medium comprises the following components: 3-10g of yeast extract powder, 8-15g of peptone, 15-25g of sucrose and 1000mL of water.
6. A Serratia marcescens fermentation broth obtained by the production process according to claim 4 or 5.
7. The use of the serratia marcescens fermentation broth of claim 6 in the preparation of a biological preparation for controlling Caffee-exterminating Kadsura longipedunculata.
8. The use of the serratia marcescens fermentation broth of claim 6 in the preparation of bacterial manure for controlling Caffea extirpati and carica papaya.
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| CN115287238A (en) * | 2022-09-09 | 2022-11-04 | 哈尔滨工业大学 | Serratia marcescens strain and application thereof |
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| CN105331566A (en) * | 2015-12-15 | 2016-02-17 | 江苏省农业科学院 | Serratia marcescens S-JS1 and application thereof |
| CN108504599A (en) * | 2018-04-10 | 2018-09-07 | 福建农林大学 | Application in Serratia bacteria strain and its production lignin-degrading enzymes and lignin degrading |
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| CN105331566A (en) * | 2015-12-15 | 2016-02-17 | 江苏省农业科学院 | Serratia marcescens S-JS1 and application thereof |
| CN108504599A (en) * | 2018-04-10 | 2018-09-07 | 福建农林大学 | Application in Serratia bacteria strain and its production lignin-degrading enzymes and lignin degrading |
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| CN115287238A (en) * | 2022-09-09 | 2022-11-04 | 哈尔滨工业大学 | Serratia marcescens strain and application thereof |
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