CN113150957A - Anti-virus invasion bottle cap combination and application thereof - Google Patents
Anti-virus invasion bottle cap combination and application thereof Download PDFInfo
- Publication number
- CN113150957A CN113150957A CN202110542737.6A CN202110542737A CN113150957A CN 113150957 A CN113150957 A CN 113150957A CN 202110542737 A CN202110542737 A CN 202110542737A CN 113150957 A CN113150957 A CN 113150957A
- Authority
- CN
- China
- Prior art keywords
- bottle
- combination
- plunger
- cap
- film
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000002155 anti-virotic effect Effects 0.000 title claims abstract description 13
- 230000009545 invasion Effects 0.000 title claims abstract description 13
- 108700028490 CAP protocol 2 Proteins 0.000 title claims abstract description 11
- 239000012528 membrane Substances 0.000 claims abstract description 20
- 238000001914 filtration Methods 0.000 claims abstract description 12
- 238000000034 method Methods 0.000 claims abstract description 10
- -1 polytetrafluoroethylene Polymers 0.000 claims description 3
- 239000004810 polytetrafluoroethylene Substances 0.000 claims description 3
- 229920001343 polytetrafluoroethylene Polymers 0.000 claims description 3
- 239000011148 porous material Substances 0.000 claims description 3
- 239000002184 metal Substances 0.000 claims description 2
- 239000004697 Polyetherimide Substances 0.000 claims 4
- 229920001601 polyetherimide Polymers 0.000 claims 4
- 239000004677 Nylon Substances 0.000 claims 2
- 239000002033 PVDF binder Substances 0.000 claims 2
- 239000004696 Poly ether ether ketone Substances 0.000 claims 2
- 239000004962 Polyamide-imide Substances 0.000 claims 2
- 239000004693 Polybenzimidazole Substances 0.000 claims 2
- 239000004695 Polyether sulfone Substances 0.000 claims 2
- 229920002873 Polyethylenimine Polymers 0.000 claims 2
- 239000004642 Polyimide Substances 0.000 claims 2
- 239000004734 Polyphenylene sulfide Substances 0.000 claims 2
- 229920001778 nylon Polymers 0.000 claims 2
- 229920002492 poly(sulfone) Polymers 0.000 claims 2
- 229920002312 polyamide-imide Polymers 0.000 claims 2
- 229920002480 polybenzimidazole Polymers 0.000 claims 2
- 229920006393 polyether sulfone Polymers 0.000 claims 2
- 229920002530 polyetherether ketone Polymers 0.000 claims 2
- 229920001721 polyimide Polymers 0.000 claims 2
- 229920000069 polyphenylene sulfide Polymers 0.000 claims 2
- 229920002981 polyvinylidene fluoride Polymers 0.000 claims 2
- 239000004033 plastic Substances 0.000 claims 1
- 229920003023 plastic Polymers 0.000 claims 1
- 239000013589 supplement Substances 0.000 abstract description 10
- 241000700605 Viruses Species 0.000 abstract description 7
- 239000007788 liquid Substances 0.000 abstract description 4
- 230000001954 sterilising effect Effects 0.000 description 11
- 239000006052 feed supplement Substances 0.000 description 9
- 238000004659 sterilization and disinfection Methods 0.000 description 9
- 239000000463 material Substances 0.000 description 5
- 238000001816 cooling Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000002572 peristaltic effect Effects 0.000 description 2
- 241001515965 unidentified phage Species 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 241000083879 Polyommatus icarus Species 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000031018 biological processes and functions Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 238000001746 injection moulding Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
Abstract
The invention discloses a bottle cap combination for preventing virus invasion and application thereof, wherein the bottle cap combination consists of 3 parts, namely a screw cap, a film clamping plunger and a filtering film; the inside cavity that is of spiral cover, its upper portion has three to connect, connects 1 and passes the bottle lid cavity, and as the interface of connecting the benefit pipe, connect 2 and connect 3 and be located the bottle lid outside, only with bottle lid cavity UNICOM, filtration membrane passes through the clamp membrane plunger and fixes to the bottle lid bottom. Before the anti-virus invasion bottle cap combination is used, filtered air is injected into the cavity through the connector 3, positive pressure exists in the cavity, viruses can be effectively prevented from entering a liquid supplement bottle or bag through the filtering membrane, and therefore the anti-virus invasion bottle cap combination is suitable for being installed on a liquid supplement device in a biological culture process.
Description
Technical Field
The invention relates to an anti-virus invasion bottle cap combination and application thereof, in particular to a high-temperature resistant anti-virus invasion bottle cap combination which can be arranged on a supplement bottle and is used for an aseptic supplement link in a biological culture process.
Background
The phage is a virus, the biological culture process, particularly the fermentation process of escherichia coli, is easily infected by the phage to cause fermentation failure, and the feeding link is a high-risk process of phage invasion.
The biological culture process, the small-scale feed supplement is realized through the feed supplement bottle, the feed supplement bottle is put into a sterilizer for high-temperature sterilization before use, the feed supplement contact pin is connected to the reactor under the aseptic condition after the sterilization is finished, and then the material is pumped into the reactor through the peristaltic pump.
The existing supplement bottle design has the following defects: 1. the bottle cap is provided with an antibacterial respirator which cannot prevent the bacteriophage from permeating; 2. because the respirator has smaller external diameter specification, operators worry that the pressure in the feed supplement is higher than the pressure which can be borne by the feed supplement bottle when sterilizing at high temperature, so the bottle cap can be unscrewed, and the bottle cap is screwed after sterilizing; 3. when the feed bottle is cooled and the feed liquid is pumped by the peristaltic pump, the pressure in the feed bottle is lower than the external environment, at the moment, the gas of the environment is inevitably fed into the feed bottle through the respirator, and the respirator can prevent bacteria in the air but can not prevent bacteriophage in the environment.
In summary, the above problems of the prior feeding bottle make it difficult to prevent the virus in the air from entering, resulting in the infection of the biological process by the virus.
Disclosure of Invention
The structure of the bottle cap is optimized, on one hand, the area of the filtering membrane is increased, so that the supplementary material bottle cap is not required to be unscrewed in the sterilization process, and the pollution caused by untimely cap tightening after sterilization is prevented; on the other hand, after sterilization is finished, compressed air is connected to the second hollow joint of the bottle cap, and air flows out from the other joint, so that on one hand, a large amount of high-pressure air can be prevented from entering the feeding bottle, and the pressure of the feeding bottle exceeds the pressure-resistant range of the bottle; on the other hand can make the cavity of spiral cover keep the malleation, when preventing cooling, pump material or infusion, produce the negative pressure in the container, this design can prevent effectively that the virus in the environment from passing through during filtration membrane gets into the container through the air-resistor.
The technical scheme of the invention is as follows: the anti-virus invasion bottle cap assembly consists of 3 parts, namely a filtering membrane, a membrane clamping plunger and a screw cap; the membrane clamping plunger fixes the filtering membrane on the screw cap, and the cavity of the screw cap is communicated with the bottom through the membrane clamping plunger and the air holes at the corresponding positions on the screw cap, so that the function of a common feed supplement bottle respirator is achieved; the rotary cover is provided with 3 hollow joints, the first hollow joint penetrates through the upper side and the lower side of the rotary cover, and the second hollow joint and the third hollow joint are communicated with the cavity from the outer side of the rotary cover; in order to further improve the sterile effect of the device, two filter heads are arranged on the second hollow joint and the first hollow joint; an O-shaped ring is added at the thread of the screw cap, and when the film clamping plunger rotates to the screw cap and the screw cap is arranged on the screw supplement bottle, the sealing effect can be achieved.
The bottle cap and the film-sandwiched plunger need high-temperature sterilization, and the material of the bottle cap and the film-sandwiched plunger is preferably metal and polytetrafluoroethylene.
Drawings
FIG. 1 is a schematic view of a combination structure of an anti-virus invading bottle cap;
FIG. 2 is a schematic view showing the combination of the anti-virus invasion bottle cap screwed on the feeding bottle.
Detailed Description
The invention will be further elucidated by means of specific embodiments, without being limited thereto, in conjunction with the accompanying drawings.
Example 1
FIG. 1 shows: an anti-virus invasion bottle cap assembly comprises 3 parts (figure 1), which are respectively a screw cap 1, a filter membrane 2 and a membrane clamping plunger 3; the bottle mouth of the blue cap bottle sold on the market at present is consistent, in order to replace the existing common blue cap bottle screw cap, the inner diameter of the first concave part is 49mm, the height of the thread is 1-7 mm, and the design is completely consistent with the inner diameter and the height of the thread; the inner diameter of the second concave part is 25mm, the thread of the second concave part and the thread 3-2 of the film clamping plunger can be mutually close and occluded, a microporous filter film with the pore diameter of 0.22 micron and the outer diameter of 25mm is selected as the filter film, the outer diameter of the film clamping plunger is designed to be 26mm, the number of the air holes 3-1 of the film clamping plunger and the number of the air holes 1-5 of the rotary cover are 5, one of the air holes passes through the center of a circle, the other 4 air holes are uniformly distributed around the center of the film clamping plunger, and the diameter of the other air holes is 5 mm; the hollow plug 1-1 has an outer diameter of 6mm and an inner diameter of 4.4mm, can be connected with a No. 15 silica gel hose sold on the market at present, and the connector 1-2 and the connector 1-3 have outer diameters of 6mm and inner diameters of 4.4mm, and can be inserted into a filter head sold on the market at present. After the above dimensions are confirmed, the molding can be completed by injection molding.
Example 2
The use method of the anti-virus invasion bottle cap combination is specifically shown in figure 1 and figure 2: firstly, placing a filtering membrane 2 with a proper size at an inward concave part at the bottom of a screw cap 1, then placing an O-shaped ring 1-6, and screwing a membrane clamping plunger 3 to the inward concave wall of the screw cap; then, connecting a material supplementing pipe at the upper part of the first hollow joint 1-1, and connecting a section of hose 6 with the height consistent with that of the bottle at the lower part of the first hollow joint; then 2 small filter heads 4 are connected with connectors 1-2 and 1-3; then, an O-shaped ring is added on the upper part of the screw threads 1-7 of the screw cap, the screw cap is firmly fixed on the screw mouth inoculation bottle 5 filled with the feed liquid, the whole feeding bottle is placed in a sterilizer, and then the sterilizer is started to sterilize the whole feeding bottle.
And after the sterilization is finished, when the temperature of the supplement bottle is reduced to 50-60 ℃, taking out the supplement bottle, immediately connecting air to a filter head on the second joint, adjusting the air flow to enable trace air to flow out from the third joint, and pumping the materials in the supplement bottle into the reactor by using a pump when the supplement bottle is cooled to room temperature.
In conclusion, by optimizing the structure of the feed supplement bottle cap combination, on one hand, the feed supplement bottle cap does not need to be unscrewed in the sterilization process by increasing the area of the filtering membrane, and the pollution caused by untimely cap tightening after sterilization is prevented; on the other hand, compressed air is connected to the filter head in the cooling process, and air in the bottle flows out from the third connector, so that on one hand, a large amount of high-pressure air can be prevented from entering the feeding bottle, the feeding bottle can generate pressure, and the bottle wall is damaged when the pressure exceeds the pressure-resistant range of the bottle; on the other hand, positive pressure can be kept in the cavity of the screw cap, negative pressure generated in the feeding bottle can be effectively prevented, and viruses in the environment can enter the feeding bottle through the filtering membrane.
The foregoing is merely an example of the embodiment of the present invention, and it should be noted that, for those skilled in the art, several modifications and variations can be made without departing from the technical principle of the present invention, and these modifications and variations should also be regarded as the protection scope of the present invention.
Claims (9)
1. The anti-virus invasion bottle cap combination is characterized by comprising 3 parts, namely a screw cap, a film clamping plunger and a filtering film; the film clamping plunger is of a cylindrical structure, the side body of the circumference of the film clamping plunger is provided with threads, and the cylinder is internally provided with air holes which are parallel to the cylindrical direction and penetrate through the upper part and the lower part; the screw cap is of a hollow cylindrical structure, the bottom of the screw cap is provided with two layers of upward concave parts, and the inner concave walls of the two layers are provided with threads; the top of the second layer is provided with an air hole parallel to the cylindrical direction and communicated with the inner cavity of the second layer; the film-sandwiched plunger is fixed on the inner concave wall of the second layer of the screw cap through threads; the filtering membrane is circular and is fixed between the membrane clamping plunger and the inner concave of the second layer of the spiral cover; the spiral cover is provided with 3 hollow connectors, the first hollow connector penetrates through the upper side and the lower side of the spiral cover, and the second hollow connector and the third hollow connector are communicated with the cavity from the outer side of the spiral cover.
2. The combination of claim 1, wherein the filter membrane has a pore size of not greater than 0.25 μm and is made of polyimide, polyetheretherketone, polyamideimide, polybenzimidazole, polyetherimide PEI, polyphenylene sulfide, nylon, polysulfone, polyethersulfone, polytetrafluoroethylene, or polyvinylidene fluoride.
3. The combination of claim 1, wherein the diameter of the filter membrane is greater than 5mm and not greater than the outer diameter of the membrane-sandwiched plunger.
4. The combination of claim 1, wherein the area of the single air hole parallel to the cylinder direction at the top of the second layer of the screw cap is not more than 100mm2The number of the air holes is not less than 1.
5. The combination of claim 1, wherein the area of the air hole of the film-sandwiched plunger is not more than 100mm2The number of the air holes is not less than 1.
6. The combination of claim 1, wherein the film-sandwiched plunger and the cap are made of metal or plastic.
7. The anti-virus entry bottle cap assembly of claim 1 further characterized in that said cap screw thread has an "O" ring at the bottom.
8. The combination of claim 1, wherein the second or third hollow joint has a filter with a pore size of 0.25 μm or less, and the filter is made of polyimide, polyetheretherketone, polyamideimide, polybenzimidazole, polyetherimide PEI, polyphenylene sulfide, nylon, polysulfone, polyethersulfone, polytetrafluoroethylene, or polyvinylidene fluoride.
9. The combination of claim 1, wherein the combination is used in a feeding bottle for a biological culture process.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110542737.6A CN113150957A (en) | 2021-05-19 | 2021-05-19 | Anti-virus invasion bottle cap combination and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110542737.6A CN113150957A (en) | 2021-05-19 | 2021-05-19 | Anti-virus invasion bottle cap combination and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113150957A true CN113150957A (en) | 2021-07-23 |
Family
ID=76876802
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110542737.6A Pending CN113150957A (en) | 2021-05-19 | 2021-05-19 | Anti-virus invasion bottle cap combination and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113150957A (en) |
Citations (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2377537Y (en) * | 1999-06-11 | 2000-05-10 | 林岗 | Blood culture bottle |
| JP2006094758A (en) * | 2004-09-29 | 2006-04-13 | Japan Science & Technology Agency | Bottle cap structure |
| US20060186076A1 (en) * | 2005-01-27 | 2006-08-24 | Amnon Shiloni | Bottle cap |
| US20090065465A1 (en) * | 2007-09-12 | 2009-03-12 | Weimin Qian | Reaction Bottle with Pressure Release |
| CN201342182Y (en) * | 2009-01-15 | 2009-11-11 | 南通杰西电器有限公司 | Anti-reflux device of liquid storage bottle of suction apparatus |
| CN202322804U (en) * | 2011-10-31 | 2012-07-11 | 江苏海华生物科技有限公司 | Feeding device of fermentation tank |
| US8316697B1 (en) * | 2010-04-19 | 2012-11-27 | Mocon, Inc. | Fixture to hermetically isolate a capped opening on a container for gas transmission testing |
| CN203975510U (en) * | 2014-07-10 | 2014-12-03 | 陈天会 | Exhaust inner cap blocks water |
| EP3375427A1 (en) * | 2015-11-13 | 2018-09-19 | Chongqing Lummy Pharmaceutical Co., Ltd. | Drug mixer, hard dual-port member, and soft infusion bag |
| US20190039792A1 (en) * | 2017-08-04 | 2019-02-07 | Canon Kabushiki Kaisha | Transfer Cap |
| CN110482012A (en) * | 2019-08-27 | 2019-11-22 | 江苏逸洁包装科技有限公司 | A kind of beverage air entrainment cap |
| CN111632179A (en) * | 2020-05-25 | 2020-09-08 | 上海履济技术服务中心 | Sterilization verifier and application thereof |
| CN211883428U (en) * | 2019-08-06 | 2020-11-10 | 宁波白蚁网络科技有限公司 | Universal scavenging cover |
| CN212981022U (en) * | 2020-06-09 | 2021-04-16 | 英诺维尔智能科技（苏州）有限公司 | Liquid infusion bottle cap suitable for automatic production of biological products |
| US20210121587A1 (en) * | 2019-10-29 | 2021-04-29 | Simai Co., Ltd. | Integrated intracavity gas circulation treatment device for laparoscopic surgery |
-
2021
- 2021-05-19 CN CN202110542737.6A patent/CN113150957A/en active Pending
Patent Citations (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2377537Y (en) * | 1999-06-11 | 2000-05-10 | 林岗 | Blood culture bottle |
| JP2006094758A (en) * | 2004-09-29 | 2006-04-13 | Japan Science & Technology Agency | Bottle cap structure |
| US20060186076A1 (en) * | 2005-01-27 | 2006-08-24 | Amnon Shiloni | Bottle cap |
| US20090065465A1 (en) * | 2007-09-12 | 2009-03-12 | Weimin Qian | Reaction Bottle with Pressure Release |
| CN201342182Y (en) * | 2009-01-15 | 2009-11-11 | 南通杰西电器有限公司 | Anti-reflux device of liquid storage bottle of suction apparatus |
| US8316697B1 (en) * | 2010-04-19 | 2012-11-27 | Mocon, Inc. | Fixture to hermetically isolate a capped opening on a container for gas transmission testing |
| CN202322804U (en) * | 2011-10-31 | 2012-07-11 | 江苏海华生物科技有限公司 | Feeding device of fermentation tank |
| CN203975510U (en) * | 2014-07-10 | 2014-12-03 | 陈天会 | Exhaust inner cap blocks water |
| EP3375427A1 (en) * | 2015-11-13 | 2018-09-19 | Chongqing Lummy Pharmaceutical Co., Ltd. | Drug mixer, hard dual-port member, and soft infusion bag |
| US20190039792A1 (en) * | 2017-08-04 | 2019-02-07 | Canon Kabushiki Kaisha | Transfer Cap |
| CN211883428U (en) * | 2019-08-06 | 2020-11-10 | 宁波白蚁网络科技有限公司 | Universal scavenging cover |
| CN110482012A (en) * | 2019-08-27 | 2019-11-22 | 江苏逸洁包装科技有限公司 | A kind of beverage air entrainment cap |
| US20210121587A1 (en) * | 2019-10-29 | 2021-04-29 | Simai Co., Ltd. | Integrated intracavity gas circulation treatment device for laparoscopic surgery |
| CN111632179A (en) * | 2020-05-25 | 2020-09-08 | 上海履济技术服务中心 | Sterilization verifier and application thereof |
| CN212981022U (en) * | 2020-06-09 | 2021-04-16 | 英诺维尔智能科技（苏州）有限公司 | Liquid infusion bottle cap suitable for automatic production of biological products |
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