CN113150687A - Anti-adhesion hydrophobic coating formula for pipette tip and production process - Google Patents
Anti-adhesion hydrophobic coating formula for pipette tip and production process Download PDFInfo
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- CN113150687A CN113150687A CN202110287776.6A CN202110287776A CN113150687A CN 113150687 A CN113150687 A CN 113150687A CN 202110287776 A CN202110287776 A CN 202110287776A CN 113150687 A CN113150687 A CN 113150687A
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- pipette tip
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09D—COATING COMPOSITIONS, e.g. PAINTS, VARNISHES OR LACQUERS; FILLING PASTES; CHEMICAL PAINT OR INK REMOVERS; INKS; CORRECTING FLUIDS; WOODSTAINS; PASTES OR SOLIDS FOR COLOURING OR PRINTING; USE OF MATERIALS THEREFOR
- C09D183/00—Coating compositions based on macromolecular compounds obtained by reactions forming in the main chain of the macromolecule a linkage containing silicon, with or without sulfur, nitrogen, oxygen, or carbon only; Coating compositions based on derivatives of such polymers
- C09D183/04—Polysiloxanes
- C09D183/08—Polysiloxanes containing silicon bound to organic groups containing atoms other than carbon, hydrogen, and oxygen
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09D—COATING COMPOSITIONS, e.g. PAINTS, VARNISHES OR LACQUERS; FILLING PASTES; CHEMICAL PAINT OR INK REMOVERS; INKS; CORRECTING FLUIDS; WOODSTAINS; PASTES OR SOLIDS FOR COLOURING OR PRINTING; USE OF MATERIALS THEREFOR
- C09D7/00—Features of coating compositions, not provided for in group C09D5/00; Processes for incorporating ingredients in coating compositions
- C09D7/40—Additives
- C09D7/60—Additives non-macromolecular
- C09D7/61—Additives non-macromolecular inorganic
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L2201/00—Properties
- C08L2201/10—Transparent films; Clear coatings; Transparent materials
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Materials Engineering (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
- Devices For Use In Laboratory Experiments (AREA)
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Abstract
The invention relates to a formula and a preparation process of an anti-adhesion hydrophobic coating for a pipette tip, wherein the hydrophobic coating formula comprises the following components in parts by weight: 20-35 parts of nano silicon dioxide, 25-40 parts of fluorosilane, 8-12 parts of polyvinyl alcohol nano microspheres, 5-20 parts of fixing agent, 2-4 parts of defoaming agent and 20-35 parts of solvent. The raw materials adopted by the invention are simple and easy to obtain, the preparation process is simple and convenient, the residue of liquid drops on the surface of the liquid transfer gun head can be effectively reduced, the accuracy of the liquid transfer volume is improved, the obtained hydrophobic coating has good transparency, the observation is convenient in the liquid taking process, and the requirement of mass production can be met by forming in a spraying mode.
Description
Technical Field
The invention relates to the technical field of pipette tips, in particular to a formula and a production process of an anti-adhesion hydrophobic coating of a pipette tip.
Background
The pipette is one kind of pipette, is used for moving a small amount of or trace reagent, is a necessary apparatus in fields such as biochemical experiment, in vitro diagnosis, etc., and the accuracy of the pipette can directly influence the test result. The existing liquid-transfering gun head is influenced by material performance, a small amount of liquid drops can be adhered to the liquid-transfering gun head when reagents are sucked and ejected, accuracy of reagent transferring is influenced, and accuracy of a detection result is reduced.
Disclosure of Invention
In order to solve the technical problems, the invention provides an anti-adhesion hydrophobic coating formula for a pipette tip and a production process thereof, and the anti-adhesion hydrophobic coating formula has the advantages of reducing reagent adhesion and improving pipetting accuracy.
In order to achieve the purpose, the technical scheme of the invention is as follows:
an anti-adhesion hydrophobic coating formula for a pipette tip comprises the following components in parts by weight: 20-35 parts of nano silicon dioxide, 25-40 parts of fluorosilane, 8-12 parts of polyvinyl alcohol nano microspheres, 5-20 parts of fixing agent, 2-4 parts of defoaming agent and 20-35 parts of solvent.
In a preferred embodiment of the present invention, the silicon fluoride is one of tridecafluorooctyltriethoxysilane and perfluorodecyltriethoxysilane.
As a preferable scheme of the invention, the particle size of the polyvinyl alcohol nano microsphere is 0.5-0.8 μm.
In a preferred embodiment of the present invention, the curing agent is one of polyamide, phenolic-modified amine, and epoxy resin.
In a preferred embodiment of the present invention, the defoaming agent is one or a combination of alkyl silicone oil, alcohol and oleic acid.
As a preferred embodiment of the present invention, the solvent is one or a combination of more of absolute ethanol, N-propanol, isopropanol, acetone, tetrahydrofuran, N-dimethylacetamide, N-dimethylformamide, toluene and xylene.
On the other hand, the invention also provides a production process of the anti-adhesion hydrophobic coating of the pipette tip, which comprises the following steps:
preparing a coating raw material: fully mixing and dissolving the components in any technical scheme, and uniformly dispersing to obtain a coating raw material;
cleaning a product: ultrasonically cleaning a pipette tip product to be coated, and drying the pipette tip product by blowing clean air at 25-40 ℃ after cleaning;
coating and curing: coating raw materials are uniformly sprayed on the inner surface and the outer surface of the pipette tip product through spraying equipment, and then the pipette tip product after being coated is placed into a drying box for natural drying and solidification so as to form a hydrophobic coating on the surface of the pipette tip product;
and (3) performance detection: randomly extracting 5-10 products from a batch of solidified pipette tip products, installing the extracted products to be detected on a pipette tip, accurately sucking 1mL of test reagent, and then transferring the test reagent into a detection container to measure the solution amount so as to judge the adhesion residual amount.
As a preferable scheme of the invention, the time length of the air-blowing drying is 10-15 min.
As a preferable scheme of the invention, the setting temperature for drying and curing is 55-65 ℃, and the curing time is 1.5-2 h.
In a preferred embodiment of the present invention, the test reagent is one or more selected from distilled water, ethanol, acetone, glycerol and Tween-20.
In conclusion, the invention has the following beneficial effects:
according to the formula and the production process of the anti-adhesion hydrophobic coating for the pipette tip, provided by the embodiment of the invention, the adopted raw materials are simple and easy to obtain, the production process is simple and convenient, the residue of liquid drops on the surface of the pipette tip can be effectively reduced, the accuracy of the pipetting volume is improved, the obtained hydrophobic coating is good in transparency, and convenient to observe in the liquid taking process, and the requirement of mass production can be met through forming in a spraying mode.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Examples
An anti-adhesion hydrophobic coating formula for a pipette tip comprises the following components in parts by weight: 20-35 parts of nano silicon dioxide, 25-40 parts of fluorosilane, 8-12 parts of polyvinyl alcohol nano microspheres, 5-20 parts of fixing agent, 2-4 parts of defoaming agent and 20-35 parts of solvent.
Specifically, the silicon fluoride is one of tridecafluorooctyl triethoxysilane and perfluorodecyl triethoxysilane, the particle size of the polyvinyl alcohol nano-microsphere is 0.5-0.8 μm, the curing agent is one of polyamide, phenolic aldehyde modified amine and epoxy resin, the defoaming agent is one or a combination of more of alkyl silicone oil, alcohols and oleic acid, and the solvent is one or a combination of more of absolute ethyl alcohol, N-propanol, isopropanol, acetone, tetrahydrofuran, N-dimethylacetamide, N-dimethylformamide, toluene and xylene.
The embodiment also provides a production process of the anti-adhesion hydrophobic coating of the pipette tip, which comprises the following steps:
preparing a coating raw material: fully mixing and dissolving the components in any technical scheme, and uniformly dispersing to obtain a coating raw material;
cleaning a product: ultrasonically cleaning a pipette tip product to be coated, and drying the pipette tip product by blowing with clean air at 25-40 ℃ for 10-15min after cleaning;
coating and curing: coating raw materials are sprayed on the inner surface and the outer surface of a pipette tip product through spraying equipment, and then the pipette tip product after being coated is placed into a drying box for natural drying and curing so as to form a hydrophobic coating on the surface of the pipette tip product, wherein the set temperature is 55-65 ℃ during drying and curing, and the curing time is 1.5-2 h;
and (3) performance detection: randomly extracting 5-10 products from a batch of solidified pipette tip products, installing the extracted products to be detected on a pipette tip, accurately sucking 1mL of test reagent, transferring the test reagent into a detection container to measure the solution amount so as to judge the adhesion residual quantity, wherein the test reagent is one or more of distilled water, ethanol, acetone, glycerol and Tween-20.
In conclusion, the invention has the following beneficial effects: the raw materials adopted are simple and easy to obtain, the production process is simple and convenient, the residue of liquid drops on the surface of the liquid transfer gun head can be effectively reduced, the accuracy of the liquid transfer volume is improved, the obtained hydrophobic coating has good transparency, the observation in the liquid taking process is facilitated, and the requirement of mass production is met through the forming in a spraying mode.
The following table shows the amounts of reagents actually obtained by using a pipette tip at room temperature of 25 ℃ to load a common pipette tip without a hydrophobic coating on the surface and a pipette tip with a hydrophobic coating on the surface, respectively, and sucking and discharging 1mL of test reagents including distilled water, ethanol, acetone, glycerol, and Tween-20.
According to the result analysis, the liquid drops adhered to the surface of the liquid transfer gun head can be effectively reduced after the hydrophobic coating is arranged, and the liquid transfer precision is improved.
Example one
An anti-adhesion hydrophobic coating formula for a pipette tip comprises the following components in parts by weight: 20 parts of nano silicon dioxide, 25 parts of fluorosilane, 8 parts of polyvinyl alcohol nano microspheres, 5 parts of fixing agent, 2 parts of defoaming agent and 20 parts of solvent.
Specifically, the silicon fluoride is tridecafluorooctyltriethoxysilane, the particle size of the polyvinyl alcohol nano-microsphere is 0.5 mu m, the curing agent is polyamide, the defoaming agent is alkyl silicone oil, and the solvent is n-propanol.
Example two
An anti-adhesion hydrophobic coating formula for a pipette tip comprises the following components in parts by weight: 30 parts of nano silicon dioxide, 35 parts of fluorosilane, 10 parts of polyvinyl alcohol nano microspheres, 10 parts of fixing agent, 3 parts of defoaming agent and 30 parts of solvent.
Specifically, the silicon fluoride is perfluorodecyl triethoxysilane, the particle size of the polyvinyl alcohol nano-microspheres is 0.6 mu m, the curing agent is phenolic aldehyde modified amine, the defoaming agent is alkyl silicone oil, and the solvent is acetone-free.
EXAMPLE III
An anti-adhesion hydrophobic coating formula for a pipette tip comprises the following components in parts by weight: 35 parts of nano silicon dioxide, 40 parts of fluorosilane, 12 parts of polyvinyl alcohol nano microspheres, 20 parts of fixing agent, 4 parts of defoaming agent and 35 parts of solvent.
Specifically, the silicon fluoride is perfluorodecyl triethoxysilane, the particle size of the polyvinyl alcohol nano-microspheres is 0.8 μm, the curing agent is epoxy resin, the defoaming agent is oleic acid, and the solvent is xylene.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (10)
1. The anti-adhesion hydrophobic coating formula for the pipette tip is characterized by comprising the following components in parts by weight: 20-35 parts of nano silicon dioxide, 25-40 parts of fluorosilane, 8-12 parts of polyvinyl alcohol nano microspheres, 5-20 parts of fixing agent, 2-4 parts of defoaming agent and 20-35 parts of solvent.
2. The pipette tip anti-adhesion hydrophobic coating formulation of claim 1, wherein the silicon fluoride is one of tridecafluorooctyltriethoxysilane and perfluorodecyltriethoxysilane.
3. The pipette tip anti-adhesion hydrophobic coating formulation of claim 1, wherein the particle size of the polyvinyl alcohol nanospheres is 0.5-0.8 μm.
4. The pipette tip anti-adhesion hydrophobic coating formulation of claim 1, wherein the curing agent is one of polyamide, phenolic modified amine, and epoxy resin.
5. The pipette tip anti-adhesion hydrophobic coating formulation of claim 1, wherein the anti-foaming agent is a combination of one or more of alkyl silicone oil, alcohols, and oleic acid.
6. The pipette tip anti-adhesion hydrophobic coating formulation of claim 1, wherein the solvent is a combination of one or more of absolute ethanol, N-propanol, isopropanol, acetone, tetrahydrofuran, N-dimethylacetamide, N-dimethylformamide, toluene, and xylene.
7. A preparation method of an anti-adhesion hydrophobic coating of a pipette tip is characterized by comprising the following steps:
preparing a coating raw material: fully mixing and dissolving the components as defined in any one of claims 1 to 6, and uniformly dispersing to obtain a coating raw material;
cleaning a product: ultrasonically cleaning a pipette tip product to be coated, and drying the pipette tip product by blowing clean air at 25-40 ℃ after cleaning;
coating and curing: coating raw materials are uniformly sprayed on the inner surface and the outer surface of the pipette tip product through spraying equipment, and then the pipette tip product after being coated is placed into a drying box for natural drying and solidification so as to form a hydrophobic coating on the surface of the pipette tip product;
and (3) performance detection: randomly extracting 5-10 products from a batch of solidified pipette tip products, installing the extracted products to be detected on a pipette tip, accurately sucking 1mL of test reagent, and then transferring the test reagent into a detection container to measure the solution amount so as to judge the adhesion residual amount.
8. The method for preparing the anti-adhesion hydrophobic coating for the pipette tip according to claim 7, wherein the drying time by blowing is 10 to 15 min.
9. The method for preparing the anti-adhesion hydrophobic coating for the pipette tip according to claim 7, wherein the temperature is set to 55-65 ℃ during drying and curing, and the curing time is 1.5-2 h.
10. The method for preparing a pipette tip anti-adhesion hydrophobic coating according to claim 7, wherein the test reagent is one or more of distilled water, ethanol, acetone, glycerol and Tween-20.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110287776.6A CN113150687A (en) | 2021-03-17 | 2021-03-17 | Anti-adhesion hydrophobic coating formula for pipette tip and production process |
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| CN202110287776.6A CN113150687A (en) | 2021-03-17 | 2021-03-17 | Anti-adhesion hydrophobic coating formula for pipette tip and production process |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115403810A (en) * | 2022-09-29 | 2022-11-29 | 浙江首康生物科技有限公司 | High-oleophobic pipette head and preparation method thereof |
| CN115433384A (en) * | 2022-09-30 | 2022-12-06 | 深圳市皆特生物科技有限公司 | Automatic conductive liquid transfer suction head and preparation process thereof |
| CN115612717A (en) * | 2022-10-20 | 2023-01-17 | 杭州瑞普晨创科技有限公司 | Accurate transfer method of trace cells |
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| CN108722511A (en) * | 2018-08-09 | 2018-11-02 | 中国标准化研究院 | Draw the liquid-transfering gun pipette tips and preparation method thereof of thick liquid |
| CN111004549A (en) * | 2019-11-22 | 2020-04-14 | 江西泉新电气有限公司 | Anticorrosive self-cleaning hydrophobic coating for glass insulator and preparation method thereof |
| CN111437898A (en) * | 2020-05-22 | 2020-07-24 | 浙江工业大学 | Gun head with super-oleophobic inner and outer walls and lipophilic gun port platform and liquid-transfering gun |
| CN112048230A (en) * | 2020-08-17 | 2020-12-08 | 湖南创瑾科技有限公司 | Super-hydrophobic coating and preparation method and application thereof |
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2021
- 2021-03-17 CN CN202110287776.6A patent/CN113150687A/en active Pending
Patent Citations (5)
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| CN108504284A (en) * | 2018-04-18 | 2018-09-07 | 北京中科东亚纳米材料科技有限公司 | A kind of preparation method of nano superhydrophobic material |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115403810A (en) * | 2022-09-29 | 2022-11-29 | 浙江首康生物科技有限公司 | High-oleophobic pipette head and preparation method thereof |
| CN115433384A (en) * | 2022-09-30 | 2022-12-06 | 深圳市皆特生物科技有限公司 | Automatic conductive liquid transfer suction head and preparation process thereof |
| CN115612717A (en) * | 2022-10-20 | 2023-01-17 | 杭州瑞普晨创科技有限公司 | Accurate transfer method of trace cells |
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Application publication date: 20210723 |
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