CN113144021A - A Chinese medicinal composition for treating drug addiction - Google Patents
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Abstract
The invention belongs to the technical field of medicines, and particularly relates to a traditional Chinese medicine composition for drug rehabilitation, which comprises 1-6 parts of pawpaw total saponin, 1-6 parts of ginkgo leaf extract and 1-6 parts of propolis according to parts by mass.
Description
Technical Field
The invention belongs to the technical field of medicines, and particularly relates to a traditional Chinese medicine composition for drug rehabilitation.
Background
Drug addiction, particularly opioid addiction, is a major global problem of drug abuse and has become a major public hazard. Repeated use of drugs by drug addicts can cause them to fall into the drug seeking place after drug rehabilitation. Addictive drugs include narcotics and psychotropic drugs. Although opioids are effective in treating pain, they are highly addictive. Addictive drugs alter neuronal cell proteins in the Central Nervous System (CNS), including G proteins, receptors, protein kinases, and cause addiction. In addition, norepinephrine, Dopamine (DA), gamma-aminobutyric acid, glutamic acid, and other substances are also involved in opioid addiction processes, and are accompanied by changes in CNS neurons at the molecular level. Studies have also shown that the DA system is involved in the psycho-dependent action of morphine, and that glutamatergic projection of the reward pathway from the prefrontal cortex and amygdala to the mesolimbic is functional in regulating dopamine neurons. Furthermore, the interaction between these DA and glutamate systems is crucial for the development of opiate reward.
Stopping behavioral and psychological consequences of drug addiction is currently the focus of drug rehabilitation researchers. For detoxification, "substitution" and "blocking" are used. "instead" of "replacing heroin with" a legitimate, low-addiction "drug" (e.g., methadone) instead of the heroin, "blocking" uses an opioid antagonist that blocks the euphoric effects of the opioid and reduces the positive potentiation of the drug, but has poor therapeutic compliance. Both methods have a fast-acting effect, but they have the disadvantages of poor safety, high recurrence rate, inherent addiction and adverse side effects. For example, methadone replacement therapy has a relapse rate as high as 93.4% after successful detoxification, which may lead to lifelong dependence.
The researches have not shown that the combined traditional Chinese medicine preparation of the pawpaw, the ginkgo leaf and the propolis has certain pharmacological action in the drug-relief animal model.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a novel traditional Chinese medicine composition for drug rehabilitation. The invention provides a traditional Chinese medicine composition for drug rehabilitation, which comprises the following components in parts by mass: 1-6 parts of pawpaw total saponin, 1-6 parts of ginkgo leaf extract and 1-6 parts of propolis.
The traditional Chinese medicine composition for drug rehabilitation preferably comprises the following components in parts by mass: 3 parts of pawpaw total saponin, 3 parts of ginkgo leaf extract and 1 part of propolis.
The preparation method of the pawpaw total saponin comprises the following steps:
(1) mature and dry fruits of pawpaw are taken as raw materials, crushed to below 24 meshes and then put into an extraction tank; adding ethanol, and extracting by vacuum heating reflux at a vacuum degree of-0.08 to-0.1 MPa and a temperature of 60 ℃; the volume dosage of the solvent is 5 times of that of the raw materials, the extraction is carried out twice, and the extraction time is 60min each time;
(2) combining the extracting solutions, concentrating under reduced pressure, wherein the concentration condition is as follows: vacuum degree range of-0.08 to-0.1 MPa, temperature of 60 ℃, and concentration to 1kg crude drug per liter;
(3) after concentration, using a sedimentation centrifuge with the rotating speed of 3000r/min and the centrifugation time of 300min for sedimentation separation;
(4) purifying by macroporous anion exchange resin: passing the supernatant after settling separation through a resin column, washing with purified water until the effluent liquid is neutral, then eluting with 50% ethanol solution, and collecting the gradient ethanol eluate with a volume 3 times of the column volume; the mass ratio of the resin to the bulk drugs is (1: 10);
(5) concentration-drying: concentrating the ethanol eluate at 60 ℃ under vacuum and reduced pressure until the relative density is 1.08-1.10, cooling the concentrated solution for 10h, filtering, and finally drying under vacuum at the temperature of below 70 ℃ to obtain the pawpaw total saponin with the oleanolic acid content of not less than 50%.
The preparation method of the ginkgo leaf extract comprises the following steps: crushing ginkgo leaves into coarse powder, heating and refluxing the ginkgo leaves with 35% ethanol which is 10 times of medicinal materials for twice extraction, merging extracting solutions, recovering ethanol, concentrating until the relative density contains 1g/ml of the medicinal materials, loading the ginkgo leaves on a macroporous resin column, washing with purified water which is 2 times of the volume of the column, sequentially eluting with 30%, 50% and 80% ethanol, collecting the ethanol eluent, recovering ethanol, concentrating until the relative density is 1.05-1.10, and performing spray drying to obtain the ginkgo leaf extract.
The propolis is wine-processed propolis, is 95% ethanol extract of original propolis, and its preparation method comprises: pulverizing propolis into coarse powder, adding 8 times of 95% ethanol, cold soaking for 24 hr, filtering, recovering ethanol from filtrate, and freeze drying.
The preparation method of the traditional Chinese medicine composition comprises the following steps: mixing fine powders of fructus Chaenomelis total saponin, folium Ginkgo extract and wine-processed propolis at a weight ratio of 3:3: 1.
Has the advantages that: the traditional Chinese medicine composition has the effect gradually superior to single administration of the pawpaw total saponin, the ginkgo leaf extract and the propolis along with the increase of the dosage, and comprises the steps of improving the withdrawal symptoms of rats, increasing the NE and 5-HT content of the rats, reducing the endorphin content, reducing the withdrawal skip reaction times of morphine-dependent mice, increasing the weight and the antioxidant level of morphine-dependent mice and the like.
The invention is completed under the funding of natural science fund (number 19KJB360001) of colleges and universities in Jiangsu province.
Detailed Description
The present invention will be described in detail with reference to specific examples.
Example 1
The preparation of the traditional Chinese medicine composition comprises the following steps: mixing fine powders of fructus Chaenomelis total saponin, folium Ginkgo extract and wine-processed propolis at a weight ratio of 3:3: 1. .
Reference substance
Clonidine hydrochloride tablets, a product of Changzhou pharmaceutical factory Co., Ltd, the national Standard H32021681, are orally administered at 75 μ g/tablet, with a common dosage of 0.3-0.9 mg, calculated as 0.9mg per day, 12 tablets per day, and a batch number of 11030714.
Jitai tablet, a product of Wuhan Hubei Chinese medicine industry Limited company, Chinese medicine standard Z20020046, orally taken, the weight of the tablet is 0.4g, 12 tablets per day, namely 4.8g per day, and batch number 110801.
When in use, the composition is prepared into required concentration by distilled water according to dosage, and is stored in a refrigerator at 4 ℃.
Application experiments
The influence of the traditional Chinese medicine composition (orally taken) on the acceleration of withdrawal of the morphine-dependent rat naloxone is observed, and the dose-effect relationship of the traditional Chinese medicine composition is considered.
First, experimental reagent and experimental apparatus
1. Reagent
Morphine hydrochloride injection, Shenyang first pharmaceutical Co., Ltd, batch No. 110301-1, pharmaceutical products of northeast China.
Naloxone hydrochloride injection, beijing kain science and technology limited, lot No. 20110401.
Norepinephrine (Norepinephrine) ELISA kit, shanghai west tang biotechnology limited, lot no: 1204181.
serotonin (Serotonin) ELISA kit, shanghai west down biotechnology limited, lot number: 1204271.
endorphin (Endorphin- β) ELISA kit, shanghai west down biotechnology limited, lot no: 1205171.
2. instrument for measuring the position of a moving object
JJ3000 animal electronic scales, products of G & G company; BS224S electronic balance (1/ten thousand), SARTORIUS product, germany; HH-6 digital display constant temperature water bath, Fuhua instruments Limited, jin Tan; Bio-RAD 680 plate reader, Burley, USA.
Second, Experimental methods
1. Grouping animals
Taking 90 SPF SD rats with half male and female parts and weight of 180-220 g, randomly dividing the SD rats into 9 groups according to the weight, wherein the groups are respectively a normal control group, a model control group, a clonidine group, a Jitai tablet group and an ultrahigh, high, medium, low and ultralow dosage group of the traditional Chinese medicine composition.
2. Dose setting
The clonidine hydrochloride tablet is based on the clinical daily dosage of 0.9mg per day according to the instruction of a medicine specification, and the clinical equivalent dose of an animal is converted by referring to a dose conversion method 'body surface area ratio' in traditional Chinese medicine pharmacology research methodology, and the clinical equivalent dose is used as the pharmacodynamic experiment dose of rats of the clonidine hydrochloride tablet. The pharmacodynamics experiment dosage of the hydrochloric acid cola tablet rat is 0.081mg kg-1The test solution is prepared by distilled water according to dosage and stored in a refrigerator at 4 ℃.
The jitai tablet is prepared by converting animal clinical equivalent dose by referring to a dose conversion method 'body surface area ratio' in 'traditional Chinese medicine pharmacology research methodology' on the basis of clinical daily dose of 12 tablets (4.8g) per day marked by a pharmaceutical instruction, and taking the clinical equivalent dose as the rat pharmacodynamics experimental dose of the jitai tablet. The pharmacodynamic experiment dose of the Jitai tablet rat is 0.432g kg-1The test solution is prepared by distilled water according to dosage and stored in a refrigerator at 4 ℃.
The Chinese medicine composition is designed into ultra-high, middle, low and ultra-low dosage groups, and the dosage is 400, 200, 100, 50 and 25mg kg-1(ig). During the experiment, the required concentration is prepared by distilled water according to the dosage, and the mixture is stored in a refrigerator at 4 ℃.
3. Experimental statistical method
All the measured data are averaged to add or subtract standard deviation
And (4) showing. The average numbers among the groups are compared by adopting One-Way ANOVA (One-Way ANOVA), the average numbers among the groups are compared pairwise, and an SNK method is adopted when the variance is uniform; dunnett's T3 method is used when variance is irregular. Finished by spssl5.0 software, α ═ 0.05.
4. Method of administration
The test drug and the positive control drug are administered by intragastric administration according to the dose, and the administration volume is 10 ml/kg-1The normal control group and the model control group are infused with 10 ml/kg of distilled water by the same method 1 time per day-1。
5. Measurement method
5.1 establishment of morphine-dependent rat model
Taking 90 SPF SD rats with half male and female, randomly dividing into 2 groups, namely a normal control group and a morphine-dependent model group, wherein the normal control group comprises 10 rats with half male and female, the morphine-dependent model group comprises 80 rats with half male and female. Morphine-dependent model group rats morphine-dependent rat models were replicated in a dose escalation method, with subcutaneous injection (sc) of morphine hydrochloride once every 12 hours (8:00am, 8:00pm) at doses ranging from 5 mg-kg each-1From the beginning, the temperature is gradually increased to 80 mg-kg each time-1The injection amount is 0.2ml 100g for the next 9 days-1. Rats in the normal control group were injected subcutaneously (sc) with an equal volume of saline.
5.2 therapeutic action of drugs on morphine-dependent rat urge withdrawal symptoms
On the 10 th day of the experiment, the morphine-dependent model group rats were deprived of morphine and naloxone (4.0mg kg. multidot.-1Ip) prompt withdrawal test, observing withdrawal reaction of rats within 30min and weight change conditions before and after (1h) prompt, scoring, injecting equal volume of physiological saline into abdominal cavities of rats in a normal control group (ip), scoring by the same method, and statistically analyzing whether a morphine-dependent model is formed or not according to a score value.
The morphine-dependent model groups, rats were divided into the following groups based on score values, if the model was successful: model control group, clonidine group, Jitai tablet group, and Chinese medicinal composition group with ultra-high, medium, low and ultra-low dosage, each group contains 10 rats with half male and female. Each group was given different treatments: firstly, a normal control group is perfused with distilled water (ig) with the same volume; ② a model control group, and equal volume of distilled water (ig) is given for intragastric administration; ③ clonidine group, the administration of clonidine by intragastric administration is 0.081 mg-kg-1(ig); 0.432 g/kg of Jitai tablets for intragastric administration-1(ig); the fifth, sixth, seventh and ninth components are Chinese medicine compositionThe dosage of the composition is 400, 200, 100, 50, 25 mg/kg-1(ig) and gavage the Chinese medicinal composition (ig) according to the dosage. Each group was administered for 5 consecutive days. Rats had free access to water and food. Naloxone (4.0mg kg) was administered 2h after d1, d3, d5 treatment-1Ip), observing the withdrawal response of the rat within 30min and the weight change condition before and after (1h) promotion, and scoring.
5.3 withdrawal symptom score
The rats are promoted to be abstinent and various abstinence symptoms are respectively graded according to the improved Suizzia grading standard: abnormal posture (2 points); high irritation: touching (1 point) and approaching (2 points); intention tremor: intermittent (1 point), continuous (2 points); biting teeth: intermittent (0.5 min), continuous (1 min); dysphoria: mild (0.5 point), obvious (1 point); lacrimation (4 points); diarrhea: soft stool (4 points), unformed (8 points); pouring water: mild (1 point), obvious (2 points); weight loss: 2 percent (0 min), 2 percent to 4 percent (5 min), 4 percent to 6 percent (10 min) and 6 percent to 8 percent (15 min).
5.4 withdrawal symptom score per rat is the sum of the individual symptom scores.
Determination of content of NE, 5-HT and endorphin in frontal cortex (PFC) of brain
d5 administration and observation, killing rat by cutting head, rapidly separating frontal cortex (PFC) brain tissue on ice bench according to rat brain stereotaxic map, accurately weighing, placing into tissue homogenizer, adding 0.1 mol.L-1Preparing 10% homogenate with perchloric acid solution in ice bath, and freezing the centrifuge at high speed at 15000 r.min-1Centrifuging at 4 deg.C for 30min, collecting supernatant, and placing into 1.5mL centrifuge tube at-80 deg.C for use. The determination was carried out according to the instructions of the NE, 5-HT, endorphin kit.
Third, experimental results
1. Influence of traditional Chinese medicine composition on withdrawal score of morphine-dependent rats
TABLE 1 morphine-dependent rat withdrawal score before dosing: (
n＝10)
Note: p <0.05, P <0.01 compared to normal controls; compared with the model control group, # P <0.05, # P < 0.01.
Table 1 shows that the withdrawal score of rats before administration was significantly increased in each of the remaining groups compared to the normal control group (P < 0.01); compared with the model control group, the withdrawal score values of rats before the administration of the drug groups are basically consistent and have no obvious difference (P > 0.05). Morphine-dependent rat models were suggested to develop.
TABLE 2 Effect of Chinese medicinal compositions on the withdrawal score of morphine dependent rats: (
n＝10)
Note: p <0.05, P <0.01 compared to normal controls;
compared with the model control group, # P <0.05, # P < 0.01;
Δ P <0.05, Δ P <0.01, compared to clonidine tablets;
it is four-star P <0.01, compared to the group of jitai tablets.
Table 2 shows that compared with the normal control group, the withdrawal score values of the model control group of d1, d3 and d5 are all significantly increased (P < 0.01); compared with the model control group, the rats with the ultra-high, medium, low and ultra-low dosage of the traditional Chinese medicine composition have obviously reduced withdrawal scores at d1, d3 and d5 stages (P <0.01 or P < 0.05).
Compared with the clonidine tablets, the withdrawal scores of rats in the low-dose group, the ultra-low-dose group and the Chinese medicinal composition in the d1 period are obviously increased (P <0.01), the withdrawal scores of rats in the ultra-low-dose group of the Chinese medicinal composition in the d3 period and the d5 period are obviously increased (P <0.05 or P <0.01), and the withdrawal scores of the rats in the rest groups are not obviously different (P > 0.05).
Compared with the Jitai tablet group, the withdrawal scores of rats in the ultrahigh, high and medium dose groups of the Chinese medicinal composition in the d1 are obviously reduced (P <0.01 or P <0.05), and the withdrawal scores of rats in the ultrahigh dose group of the Chinese medicinal composition in the d3 are obviously reduced (P < 0.05). The traditional Chinese medicine composition has better effect than Jitai tablets with the increase of the dosage.
With the increase of the dosage, the effect of the traditional Chinese medicine composition is gradually superior to that of single administration of the common floweringquince fruit total saponin, the ginkgo leaf extract and the propolis. The compatibility and combination have certain improvement effect on the treatment effect of the withdrawal of rats.
2. Influence of traditional Chinese medicine composition on morphine-dependent rat frontal cortex NE, 5-HT and endorphin content
TABLE 3 Effect of Chinese medicinal composition on morphine-dependent rat frontal cortex NE, 5-HT, endorphin content: (
n＝10)
Note: p <0.05, P <0.01 compared to normal controls;
compared with the model control group, # P <0.05, # P < 0.01;
Δ P <0.05, Δ P <0.01, compared to clonidine tablets;
it is four-star P <0.01, compared to the group of jitai tablets.
Table 3 shows that the NE and 5-HT content of the rats in the model control group is obviously increased (P <0.01) and the endorphin content is obviously reduced (P <0.01) compared with the normal control group. Compared with a model control group, the NE and 5-HT content of rats in the traditional Chinese medicine composition with ultra-high, medium, low and ultra-low doses is obviously reduced (P <0.01 or P <0.05), and the endorphin content is obviously increased (P < 0.01).
Compared with clonidine tablets, the NE content of the rats in the low and ultra-low dose groups of the traditional Chinese medicine composition is obviously increased (P <0.01), and the rest rats have no obvious difference (P > 0.05).
Compared with the Jitai tablet group, the NE content of the rats with the ultra-high, high and medium dosage of the traditional Chinese medicine composition is obviously reduced (P <0.01 or P <0.05), and the rest rats have no obvious difference (P > 0.05). But the effect is gradually superior to that of Jitai tablets with the increase of dosage.
Compared with the administration group with single ingredient of pawpaw total saponin, ginkgo leaf extract and propolis, the content of NE and 5-HT of the traditional Chinese medicine composition is obviously increased, and the content of endorphin is also obviously reduced. Shows that the compatibility of medicines has certain effect on improving the indexes of the content of NE, 5-HT and endorphin.
Example 2
The preparation of the traditional Chinese medicine composition comprises the following steps: mixing fine powders of fructus Chaenomelis total saponin, folium Ginkgo extract and wine-processed propolis at a weight ratio of 3:3: 1.
Application experiments
The influence of the traditional Chinese medicine composition (orally taken) on the acceleration of withdrawal of the morphine-dependent naloxone by mice is observed, and the dose-effect relationship of the traditional Chinese medicine composition is considered.
First, the experimental reagents and instruments are the same as those in example 1.
Second, Experimental methods
1. Grouping animals
70 SPF-grade NIH mice with half weight of 18-22 g are selected, and randomly divided into 7 groups according to the weight, wherein the 7 groups are respectively a normal control group, a model control group, a clonidine group, a Jitai tablet group and a high-dose, medium-dose and low-dose group of the traditional Chinese medicine composition.
2. Dose setting
The clonidine hydrochloride tablet is based on the clinical daily dosage of 0.9mg per day according to the instruction of a medicine specification, and the clinical equivalent dose of an animal is converted by referring to a dose conversion method 'body surface area ratio' in traditional Chinese medicine pharmacology research methodology, and the clinical equivalent dose is used as the pharmacodynamic experiment dose of a clonidine hydrochloride tablet mouse. The pharmacodynamics experiment dosage of the clonidine hydrochloride tablet for the mouse is 0.117mg kg-1The test solution is prepared by distilled water according to dosage and stored in a refrigerator at 4 ℃.
Jitai tablet, based on the daily clinical dosage of 12 tablets (4.8g) per day marked by the pharmaceutical instruction, refer to the dosage conversion method in the methodology of pharmacological research of Chinese medicineThe body surface area ratio is converted into animal clinical equivalent dose, and the clinical equivalent dose is used as the pharmacodynamic experiment dose of the Jitai tablet mouse. The pharmacodynamics experiment dosage of the Jitai tablet mouse is 0.624g kg-1The test solution is prepared by distilled water according to dosage and stored in a refrigerator at 4 ℃.
The traditional Chinese medicine composition is designed to have high pharmacodynamic mouse dose of 200, 100 and 50 mg/kg-1. During the experiment, the required concentration is prepared by distilled water according to the dosage, and the mixture is stored in a refrigerator at 4 ℃.
3 statistical method of experiment
All measurements are expressed as means plus standard deviations (x. + -.s). The average numbers among the groups are compared by adopting One-Way ANOVA (One-Way ANOVA), the average numbers among the groups are compared pairwise, and an SNK method is adopted when the variance is uniform; dunnett's T3 method is used when variance is irregular. Finished by spssl5.0 software, α ═ 0.05.
4 method of administration
The test drug and the positive control drug are administered by intragastric administration according to the dose, and the administration volume is 20 ml/kg-1Administering 20 ml/kg of distilled water to normal control group and model control group by intragastric administration for 1 time per day-1。
5 measurement method
70 SPF-grade NIH mice with 18-22 g of half of each sex are randomly divided into 7 groups, namely a normal control group, a model control group, a clonidine group, a Jitai group and a traditional Chinese medicine composition high, medium and low dose group. Except the normal control group, all the mice of the other groups are injected with morphine subcutaneously, the normal control group is injected with equal volume of 0.9 percent sodium chloride injection subcutaneously for 6 days continuously, the dosage of the normal control group is 5mg/kg on the 1 st day, the dosage is increased by one time every day, the dosage reaches 160mg/kg on the 6 th day, and a mouse morphine dependence model is established. Meanwhile, each administration group is intragastrically administered according to the dose, and the normal control group and the model control group are intragastrically administered with equal volume of distilled water. After the last administration for 45min, each group of mice was injected with naloxone 6mg/kg intraperitoneally, the frequency of the jump reaction occurred in the mice within 30min was measured, and the change in body mass of the mice before and after the jump reaction for 1h was compared.
Mice body mass before naloxone injection-mice body mass after skip reaction
6 serum SOD, MDA, GSH-PX content
And 24h after the last administration, taking the serum of the mouse and storing for later use. The contents of superoxide dismutase (SOD), Malondialdehyde (MDA) and glutathione peroxidase (GSH-PX) in serum were determined by a kit method (ELISA method).
Third, experimental results
TABLE 4 Effect of Chinese medicinal composition on morphine-dependent mouse withdrawal promotion (
n＝10)
Note: p <0.05, P <0.01 compared to normal controls;
compared with the model control group, # P <0.05, # P < 0.01;
Δ P <0.05, Δ P <0.01, compared to clonidine tablets;
it is four-star P <0.01, compared to the group of jitai tablets.
Table 4 shows that compared with the normal control group, the number of jumping reactions of the mice in the model control group is significantly increased (P <0.01), and the decrease of body mass is significantly increased (P < 0.01); compared with a model control group, the times of jumping reactions of mice in high, medium and low dose groups of the traditional Chinese medicine composition are obviously reduced (P <0.01 or P <0.05), and the reduction of physical quality is obviously reduced (P < 0.01).
Compared with clonidine tablets, the frequency of jumping reactions of mice in a high-dose group of the traditional Chinese medicine composition is obviously reduced (P <0.05), and the decline of physical quality is obviously reduced (P < 0.01).
Compared with the Jitai tablet group, the Chinese medicinal composition has the advantages that the frequency of jumping reaction of mice in a high-dose group is obviously reduced (P <0.05), and the decline of physical quality is obviously reduced (P < 0.01).
The results show that the high, medium and low doses of the traditional Chinese medicine composition have a certain dose-effect relationship on reducing the frequency of withdrawal and skip reaction of morphine-dependent mice and reducing the decline of physical quality, and the effect is superior to that of cola tablets and Jitai tablets.
The influence of high, medium and low doses of the traditional Chinese medicine composition on reducing the number of times of withdrawal and skip reaction of morphine-dependent mice and reducing the decline of physical quality is obviously superior to that of single administration of common floweringquince fruit total saponin, ginkgo leaf extract and propolis. The Chinese medicinal composition after compatibility has certain treatment effect on drug rehabilitation.
TABLE 5 comparison of SOD, MDA, GSH-PX levels in sera of mice of each group
Note: p <0.05, P <0.01 compared to normal controls;
compared with the model control group, # P <0.05, # P < 0.01;
Δ P <0.05, Δ P <0.01, compared to clonidine tablets;
it is four-star P <0.01, compared to the group of jitai tablets.
Table 5 shows that compared with the normal control group, the mouse SOD and GSH-PX activity of the model control group is reduced, and the MDA content is obviously increased; compared with the model control group, the traditional Chinese medicine composition has the advantages that the SOD and GSH-PX activities of the high, medium and low dosage groups are obviously improved, and the MDA content is obviously reduced.
Compared with clonidine tablet, the traditional Chinese medicine composition has obviously improved SOD and GSH-PX activities (P <0.05) in high, medium and low dosage groups, and has obviously reduced MDA content (P < 0.05).
Compared with Jitai tablet, the traditional Chinese medicine composition has obviously improved SOD and GSH-PX activities (P <0.05) in high, medium and low dosage groups, and obviously reduced MDA content (P < 0.05).
The results show that the high, medium and low dosage of the traditional Chinese medicine composition has a certain dose-effect relationship on increasing the activities of SOD and GSH-PX and reducing the content of MDA, and the effect is superior to that of cola tablets and Jitai tablets.
Meanwhile, as can be seen from table 5, the activities of SOD and GSH-PX in the high, medium and low dose groups of the traditional Chinese medicine composition are higher than that of the single-use pawpaw total saponin or ginkgo leaf extract or propolis, while the MDA content is obviously lower than that of the single-use pawpaw total saponin or ginkgo leaf extract or propolis, which indicates that the traditional Chinese medicine composition after compatibility has obvious advantages in antioxidant level.
Claims (5)
1. A traditional Chinese medicine composition for drug rehabilitation is characterized by comprising the following components in parts by mass: 1-6 parts of pawpaw total saponin, 1-6 parts of ginkgo leaf extract and 1-6 parts of propolis.
2. The traditional Chinese medicine composition for drug rehabilitation according to claim 1, which comprises the following components in parts by mass: 3 parts of pawpaw total saponin, 3 parts of ginkgo leaf extract and 1 part of propolis.
3. The Chinese medicinal composition for detoxification according to claim 1, wherein the preparation method of the pawpaw total saponins comprises the following steps:
(1) mature and dry fruits of pawpaw are taken as raw materials, crushed to below 24 meshes and then put into an extraction tank; adding a solvent, and extracting by vacuum heating reflux at a vacuum degree of-0.08 to-0.1 MPa and a temperature of 50 to 80 ℃; the volume dosage of the solvent is 4 times of that of the raw materials, the extraction is carried out at least twice, and the extraction time is at least 60min each time;
(2) combining the extracting solutions, concentrating under reduced pressure, wherein the concentration condition is as follows: vacuum degree range of-0.08 to-0.1 MPa, temperature not higher than 75 ℃, and concentration to 0.5 to 1kg crude drug per liter;
(3) after concentration, using a sedimentation centrifuge with the rotating speed of 3000r/min and the centrifugation time of 300min for sedimentation separation;
(4) purifying by macroporous anion exchange resin: passing the supernatant after the sedimentation separation through a resin column, washing with purified water until the effluent liquid is neutral, then eluting with 30-50% ethanol solution, and collecting the gradient ethanol eluate with the volume 3 times of the column volume; the mass ratio of the resin to the bulk drugs is (1: 10);
(5) concentration-drying: and (3) concentrating the ethanol eluent under vacuum reduced pressure at the temperature of not higher than 75 ℃ until the relative density is 1.08-1.10, cooling the concentrated solution for 8-12 h, filtering, and finally drying under vacuum at the temperature of below 70 ℃ to obtain the pawpaw total saponin with the oleanolic acid content of not less than 50%.
4. The Chinese medicinal composition for detoxification according to claim 1, wherein the preparation method of the ginkgo biloba leaf extract comprises the following steps: crushing ginkgo leaves into coarse powder, heating and refluxing the ginkgo leaves with 35% ethanol in an amount which is 10 times that of medicinal materials for twice extraction, combining extracting solutions, recovering ethanol, concentrating until the relative density contains 1g/ml of the medicinal materials, loading the extract on a macroporous resin column, washing with purified water in an amount which is 2 times that of the column, sequentially eluting with 30%, 50% and 80% ethanol, collecting the ethanol eluent, recovering ethanol, concentrating until the relative density is 1.05-1.10, and performing spray drying to obtain the ginkgo leaf extract.
5. The Chinese medicinal composition for detoxification according to claim 1, wherein the propolis is wine-processed propolis which is 95% ethanol extract of original propolis, and the preparation method comprises: pulverizing propolis into coarse powder, adding 8 times of 95% ethanol, cold soaking for 24 hr, filtering, recovering ethanol from filtrate, and freeze drying.
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| CN1280499A (en) * | 1997-12-03 | 2001-01-17 | 科学研究与运用咨询公司 | Use of ginkgo biloba extracts for preparing a medicine |
| CN1704091A (en) * | 2004-06-04 | 2005-12-07 | 杨海龙 | Traditional Chinese medicine for stopping drug-taking |
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| CN1280499A (en) * | 1997-12-03 | 2001-01-17 | 科学研究与运用咨询公司 | Use of ginkgo biloba extracts for preparing a medicine |
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