CN113109478A - Method for detecting biogenic amine content in dried shrimps - Google Patents
Method for detecting biogenic amine content in dried shrimps Download PDFInfo
- Publication number
- CN113109478A CN113109478A CN202110416167.6A CN202110416167A CN113109478A CN 113109478 A CN113109478 A CN 113109478A CN 202110416167 A CN202110416167 A CN 202110416167A CN 113109478 A CN113109478 A CN 113109478A
- Authority
- CN
- China
- Prior art keywords
- biogenic amine
- dried shrimps
- content
- container
- detecting
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 150000001412 amines Chemical class 0.000 title claims abstract description 83
- 230000000035 biogenic effect Effects 0.000 title claims abstract description 83
- 241000143060 Americamysis bahia Species 0.000 title claims abstract description 75
- 238000000034 method Methods 0.000 title claims abstract description 44
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 44
- 239000001301 oxygen Substances 0.000 claims abstract description 44
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 44
- 238000002791 soaking Methods 0.000 claims abstract description 36
- 238000001514 detection method Methods 0.000 claims abstract description 31
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims abstract description 22
- 238000006243 chemical reaction Methods 0.000 claims abstract description 22
- 239000011949 solid catalyst Substances 0.000 claims abstract description 20
- 239000000523 sample Substances 0.000 claims abstract description 13
- 238000010438 heat treatment Methods 0.000 claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 17
- 239000008213 purified water Substances 0.000 claims description 16
- 241000238557 Decapoda Species 0.000 claims description 12
- 102000004316 Oxidoreductases Human genes 0.000 claims description 10
- 108090000854 Oxidoreductases Proteins 0.000 claims description 10
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 claims description 7
- 238000004128 high performance liquid chromatography Methods 0.000 claims description 6
- 238000007789 sealing Methods 0.000 claims description 6
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical group O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 5
- 238000005485 electric heating Methods 0.000 claims description 5
- 125000001931 aliphatic group Chemical group 0.000 claims description 4
- 125000003277 amino group Chemical group 0.000 claims description 4
- 125000000623 heterocyclic group Chemical group 0.000 claims description 4
- 239000000126 substance Substances 0.000 claims description 4
- 150000002148 esters Chemical group 0.000 claims 1
- 230000006698 induction Effects 0.000 claims 1
- 230000009286 beneficial effect Effects 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 3
- 239000002904 solvent Substances 0.000 abstract description 3
- 125000002723 alicyclic group Chemical group 0.000 description 3
- 238000006555 catalytic reaction Methods 0.000 description 3
- 238000009434 installation Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 1
- 229910001882 dioxygen Inorganic materials 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/44—Sample treatment involving radiation, e.g. heat
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N31/00—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
- G01N31/10—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods using catalysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/02—Food
- G01N33/12—Meat; Fish
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N2030/022—Column chromatography characterised by the kind of separation mechanism
- G01N2030/027—Liquid chromatography
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Food Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
The application discloses a method for detecting the content of biogenic amine in dried shrimps, which comprises the steps of extending a special probe of a biosensor into a soaking solution in a container, and carrying out a specific reaction with biogenic amine in the soaking solution; putting a solid catalyst into the sealed container, and reacting the solid catalyst with the generated hydrogen peroxide to release oxygen; detecting the oxygen content by an oxygen content detector; and the data is transmitted to an operation system of the intelligent host to obtain the quantity of the biogenic amine and the content percentage of the biogenic amine in the dried shrimps. The beneficial effect of this application is: by adopting the method of soaking dried shrimps, biogenic amine in the dried shrimps can be completely dissolved, and a solvent heating structure is added, so that the soaking time is shortened, and the detection efficiency is improved; the method is beneficial to judging whether the detection reaction is complete, achieves the effect of full reaction, is convenient to quickly judge whether the dried shrimps are qualified or not and react with different kinds of biogenic amines, and improves the detection application range.
Description
Technical Field
The application relates to a method for detecting biogenic amine content in dried shrimps.
Background
Dried shrimps, namely fresh live shrimps, are directly dried to become dried shrimps, and some small shrimps are dried to be called as dried shrimps, but for example, Roche shrimps and bamboo-joint shrimps are dried to become large heads, fresh and delicious in meat quality and fresh and fragrant in smell, so the dried shrimps are called as dried shrimps, are soaked in warm water firstly when being eaten and then cooked in a favorite way, and are dried and stored after a large amount of fresh river shrimps or sea shrimps are caught by fishermen and eaten at once, and most of the existing dried shrimps are specially manufactured for storage and convenient carrying.
The content of biogenic amine in the dried shrimps exceeds the standard, so that physical damage can be caused to consumers, a sampling laboratory is adopted, the detection time is determined by a detection method, the detection efficiency is influenced by long time, meanwhile, different types of biogenic amine can be added into the dried shrimps in different manufacturing processes, so that different detection methods are required, and the limitation exists. Therefore, a method for detecting the content of biogenic amine in dried shrimps is provided aiming at the problems.
Disclosure of Invention
The present application aims to solve the above problems and provide a method for detecting the content of biogenic amine in dried shrimps.
The purpose is realized through the following technical scheme, and the method for detecting the content of the biogenic amine in the dried shrimps comprises the following specific steps:
step 1: sealing a certain amount of dried shrimps in a container, injecting a certain amount of purified water, soaking for 3-4 minutes, and turning over the container once or twice during the soaking;
step 2: extending a special probe of the biosensor into a soaking solution in a container to perform a specific reaction with biogenic amine in the soaking solution;
and step 3: putting a solid catalyst into the sealed container, and reacting the solid catalyst with the generated hydrogen peroxide to release oxygen;
and 4, step 4: detecting the oxygen content by an oxygen content detector;
and 5: according to the oxygen amount and 2H measured in the step 42O2＝2H2O+O2×) which is transmitted to an operation system of the intelligent host through data to obtain the amount of the biogenic amine and the content percentage of the biogenic amine in the dried shrimps;
and 6, measuring the content of the shrimp biogenic amine in the storage process by using a high performance liquid chromatography method set forth by the national standard, wherein the measured content of the shrimp biogenic amine is used as a comparison standard of the result of the step 5.
Preferably, the container, the biosensor, the oxygen content detector and the intelligent host are assembled to form a complete portable detector, the container is of a transparent silica gel structure, and a telescopic structure for installing solid catalyst is arranged in the container.
Preferably, in step 1, an electric heating structure is arranged at the bottom in the container, and the heating temperature is controlled to be 30-40 ℃.
Preferably, in the step 1, 2 grams of dried shrimps are weighed, the taking amount of the purified water accounts for two thirds of the space of the specification container, and the dried shrimps are completely immersed in the purified water.
Preferably, in step 2, the biosensor is electrically connected to the converter, and detects the change of the signal after passing through the converter, and determines whether the reaction of the biogenic amine in the soak solution is sufficient according to the change of the detection signal.
Preferably, in step 2, purified amine oxidase is immobilized on the special probe of the biosensor, and the detection operation is performed by using the chemical principle that the amine oxidase catalyzes the removal of the amino group of biogenic amine to form hydrogen peroxide.
Preferably, the biogenic amine in the dried shrimps can be any one of aliphatic, alicyclic and heterocyclic.
Preferably, in step 4, the detection of the oxygen content detector is performed synchronously with step 3.
Preferably, in step 4, the oxygen content detector is electrically connected with the intelligent host, and the sensing detection area of the oxygen content detector is located inside the container.
Preferably, the solid catalyst gradually extends into the soaking liquid through a telescopic structure arranged in the container.
The beneficial effect of this application is: by adopting the method of soaking dried shrimps, biogenic amine in the dried shrimps can be completely dissolved, and a solvent heating structure is added, so that the soaking time is shortened, and the detection efficiency is improved; the method is beneficial to judging whether the detection reaction is complete or not, achieves the effect of full reaction, can obtain the content of oxygen generated by the reaction, and obtains the content of biogenic amine according to calculation;
by storing the safety detection standard data of the international biogenic amine content in the memory of the intelligent host, a comparison database can be formed, and whether the dried shrimps are qualified or not can be conveniently and quickly judged; the purified amine oxidase is fixed on the special probe of the biosensor, so that the reaction of different kinds of biogenic amines is facilitated, and the detection application range is improved.
Detailed Description
The technical solutions in the embodiments of the present application will be described clearly and completely below, and it should be apparent that the described embodiments are only a part of the embodiments of the present application, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
The first embodiment is as follows:
a method for detecting the content of biogenic amine in dried shrimps comprises the following specific steps:
step 1: sealing a certain amount of dried shrimps in a container, injecting a certain amount of purified water, soaking for 3-4 minutes, and turning over the container once or twice during the soaking;
step 2: extending a special probe of the biosensor into a soaking solution in a container to perform a specific reaction with biogenic amine in the soaking solution;
and step 3: putting a solid catalyst into the sealed container, and reacting the solid catalyst with the generated hydrogen peroxide to release oxygen;
and 4, step 4: detecting the oxygen content by an oxygen content detector;
and 5: according to the oxygen amount and 2H measured in the step 42O2＝2H2O+O2×) which is transmitted to an operation system of the intelligent host through data to obtain the amount of the biogenic amine and the content percentage of the biogenic amine in the dried shrimps;
and 6, measuring the content of the shrimp biogenic amine in the storage process by using a high performance liquid chromatography method set forth by the national standard, wherein the measured content of the shrimp biogenic amine is used as a comparison standard of the result of the step 5.
Further, container, biosensor, oxygen content detector and intelligent host assembly form a complete portable detector, and the container is the transparent silica gel structure, set up the extending structure of installation solid catalysis in the container.
Further, in step 1, an electric heating structure is arranged at the bottom in the container, and the heating temperature is controlled to be 30-40 ℃.
Further, in the step 1, 2 grams of dried shrimps are weighed, the taking amount of the purified water accounts for two thirds of the space of the specification container, and the dried shrimps are completely immersed in the purified water.
Further, the solid catalyst gradually extends into the soaking liquid through a telescopic structure arranged in the container.
The method for detecting the content of the biogenic amine in the dried shrimps has the advantages that: by adopting the method of soaking dried shrimps, biogenic amine in the dried shrimps can be completely dissolved, and a solvent heating structure is added, so that the soaking time is shortened, and the detection efficiency is improved.
Example two:
a method for detecting the content of biogenic amine in dried shrimps comprises the following specific steps:
step 1: sealing a certain amount of dried shrimps in a container, injecting a certain amount of purified water, soaking for 3-4 minutes, and turning over the container once or twice during the soaking;
step 2: extending a special probe of the biosensor into a soaking solution in a container to perform a specific reaction with biogenic amine in the soaking solution;
and step 3: putting a solid catalyst into the sealed container, and reacting the solid catalyst with the generated hydrogen peroxide to release oxygen;
and 4, step 4: detecting the oxygen content by an oxygen content detector;
and 5: according to the oxygen amount and 2H measured in the step 42O2＝2H2O+O2×) which is transmitted to an operation system of the intelligent host through data to obtain the amount of the biogenic amine and the content percentage of the biogenic amine in the dried shrimps;
and 6, measuring the content of the shrimp biogenic amine in the storage process by using a high performance liquid chromatography method set forth by the national standard, wherein the measured content of the shrimp biogenic amine is used as a comparison standard of the result of the step 5.
Further, container, biosensor, oxygen content detector and intelligent host assembly form a complete portable detector, and the container is the transparent silica gel structure, set up the extending structure of installation solid catalysis in the container.
Further, in step 2, the biosensor is electrically connected to the converter, and the change of the signal is detected after passing through the converter, and whether the reaction of the biogenic amine in the soak solution is sufficient is determined according to the change condition of the detection signal.
Further, in step 4, the oxygen content detector is electrically connected with the intelligent host, and the sensing detection area of the oxygen content detector is located inside the container.
The method for detecting the content of the biogenic amine in the dried shrimps has the advantages that: the method is beneficial to judging whether the detection reaction is complete or not, achieves the effect of full reaction, can obtain the content of oxygen generated by the reaction, and obtains the content of biogenic amine according to calculation.
Example three:
a method for detecting the content of biogenic amine in dried shrimps comprises the following specific steps:
step 1: sealing a certain amount of dried shrimps in a container, injecting a certain amount of purified water, soaking for 3-4 minutes, and turning over the container once or twice during the soaking;
step 2: extending a special probe of the biosensor into a soaking solution in a container to perform a specific reaction with biogenic amine in the soaking solution;
and step 3: putting a solid catalyst into the sealed container, and reacting the solid catalyst with the generated hydrogen peroxide to release oxygen;
and 4, step 4: detecting the oxygen content by an oxygen content detector;
and 5: according to the oxygen amount and 2H measured in the step 42O2＝2H2O+O2×) which is transmitted to an operation system of the intelligent host through data to obtain the amount of the biogenic amine and the content percentage of the biogenic amine in the dried shrimps;
and 6, measuring the content of the shrimp biogenic amine in the storage process by using a high performance liquid chromatography method set forth by the national standard, wherein the measured content of the shrimp biogenic amine is used as a comparison standard of the result of the step 5.
Further, in step 1, an electric heating structure is arranged at the bottom in the container, and the heating temperature is controlled to be 30-40 ℃.
Further, in the step 1, 2 grams of dried shrimps are weighed, the taking amount of the purified water accounts for two thirds of the space of the specification container, and the dried shrimps are completely immersed in the purified water.
Further, in step 2, the biosensor is electrically connected to the converter, and the change of the signal is detected after passing through the converter, and whether the reaction of the biogenic amine in the soak solution is sufficient is determined according to the change condition of the detection signal.
Further, in step 2, purified amine oxidase is immobilized on a special probe of the biosensor, and detection operation is performed by using a chemical principle that the amine oxidase catalyzes and removes an amino group of biogenic amine to form hydrogen peroxide.
Further, the biogenic amine in the dried shrimps can be any one of aliphatic, alicyclic and heterocyclic.
The method for detecting the content of the biogenic amine in the dried shrimps has the advantages that: the safety detection standard data of the international biogenic amine content are stored in the memory of the intelligent host, so that a comparison database can be formed, and whether the dried shrimps are qualified or not can be judged conveniently and quickly.
Example four:
a method for detecting the content of biogenic amine in dried shrimps comprises the following specific steps:
step 1: sealing a certain amount of dried shrimps in a container, injecting a certain amount of purified water, soaking for 3-4 minutes, and turning over the container once or twice during the soaking;
step 2: extending a special probe of the biosensor into a soaking solution in a container to perform a specific reaction with biogenic amine in the soaking solution;
and step 3: putting a solid catalyst into the sealed container, and reacting the solid catalyst with the generated hydrogen peroxide to release oxygen;
and 4, step 4: detecting the oxygen content by an oxygen content detector;
and 5: oxygen gas measured according to step 4Amount and 2H2O2＝2H2O+O2×) which is transmitted to an operation system of the intelligent host through data to obtain the amount of the biogenic amine and the content percentage of the biogenic amine in the dried shrimps;
and 6, measuring the content of the shrimp biogenic amine in the storage process by using a high performance liquid chromatography method set forth by the national standard, wherein the measured content of the shrimp biogenic amine is used as a comparison standard of the result of the step 5.
Further, container, biosensor, oxygen content detector and intelligent host assembly form a complete portable detector, and the container is the transparent silica gel structure, set up the extending structure of installation solid catalysis in the container.
Further, in step 1, an electric heating structure is arranged at the bottom in the container, and the heating temperature is controlled to be 30-40 ℃.
Further, in the step 1, 2 grams of dried shrimps are weighed, the taking amount of the purified water accounts for two thirds of the space of the specification container, and the dried shrimps are completely immersed in the purified water.
Further, in step 2, the biosensor is electrically connected to the converter, and the change of the signal is detected after passing through the converter, and whether the reaction of the biogenic amine in the soak solution is sufficient is determined according to the change condition of the detection signal.
Further, in step 2, purified amine oxidase is immobilized on a special probe of the biosensor, and detection operation is performed by using a chemical principle that the amine oxidase catalyzes and removes an amino group of biogenic amine to form hydrogen peroxide.
Further, the biogenic amine in the dried shrimps can be any one of aliphatic, alicyclic and heterocyclic.
Further, in step 4, the detection of the oxygen content detector is performed synchronously with step 3.
Further, in step 4, the oxygen content detector is electrically connected with the intelligent host, and the sensing detection area of the oxygen content detector is located inside the container.
Further, the solid catalyst gradually extends into the soaking liquid through a telescopic structure arranged in the container.
The method for detecting the content of the biogenic amine in the dried shrimps has the advantages that: the purified amine oxidase is fixed on the special probe of the biosensor, so that the reaction of different kinds of biogenic amines is facilitated, and the detection application range is improved.
It will be evident to those skilled in the art that the present application is not limited to the details of the foregoing illustrative embodiments, and that the present application may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the application being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (10)
1. A method for detecting the content of biogenic amine in dried shrimps is characterized by comprising the following steps: the method comprises the following specific steps:
step 1: sealing a certain amount of dried shrimps in a container, injecting a certain amount of purified water, soaking for 3-4 minutes, and turning over the container once or twice during the soaking;
step 2: extending a special probe of the biosensor into a soaking solution in a container to perform a specific reaction with biogenic amine in the soaking solution;
and step 3: putting a solid catalyst into the sealed container, and reacting the solid catalyst with the generated hydrogen peroxide to release oxygen;
and 4, step 4: detecting the oxygen content by an oxygen content detector;
and 5: according to the oxygen amount and 2H measured in the step 42O2＝2H2O+O2×) which is transmitted to an operation system of the intelligent host through data to obtain the amount of the biogenic amine and the content percentage of the biogenic amine in the dried shrimps;
and 6, measuring the content of the shrimp biogenic amine in the storage process by using a high performance liquid chromatography method set forth by the national standard, wherein the measured content of the shrimp biogenic amine is used as a comparison standard of the result of the step 5.
2. The method for detecting the content of the biogenic amine in the dried shrimps as claimed in claim 1, characterized in that: the container, the biosensor, the oxygen content detector and the intelligent host are assembled to form a complete portable detector, the container is of a transparent silica gel structure, and a telescopic structure for installing solid catalyst is arranged in the container.
3. The method for detecting the content of the biogenic amine in the dried shrimps as claimed in claim 1, characterized in that: in the step 1, an electric heating structure is arranged at the bottom in the container, and the heating temperature is controlled to be 30-40 ℃.
4. The method for detecting the content of the biogenic amine in the dried shrimps as claimed in claim 1, characterized in that: in the step 1, 2 grams of dried shrimps are weighed, the taking amount of the purified water accounts for two thirds of the space of the specification container, and the dried shrimps are completely immersed by the purified water.
5. The method for detecting the content of the biogenic amine in the dried shrimps as claimed in claim 1, characterized in that: in the step 2, the biosensor is electrically connected with the converter, the change of the signal is detected after passing through the converter, and whether the reaction of the biogenic amine in the soak solution is sufficient or not is judged according to the change condition of the detection signal.
6. The method for detecting the content of the biogenic amine in the dried shrimps as claimed in claim 1, characterized in that: in the step 2, purified amine oxidase is fixed on a special probe of the biosensor, and detection operation is implemented by utilizing the chemical principle that the amine oxidase catalyzes and removes the amino group of biogenic amine to form hydrogen peroxide.
7. The method for detecting the content of the biogenic amine in the dried shrimps as claimed in claim 1, characterized in that: the biogenic amine in the dried shrimps can be any one of aliphatic series, ester ring series and heterocyclic ring series.
8. The method for detecting the content of the biogenic amine in the dried shrimps as claimed in claim 1, characterized in that: in step 4, the detection of the oxygen content detector is performed synchronously with step 3.
9. The method for detecting the content of the biogenic amine in the dried shrimps as claimed in claim 1, characterized in that: in step 4, the oxygen content detector is electrically connected with the intelligent host, and the induction detection area of the oxygen content detector is positioned inside the container.
10. The method for detecting the content of the biogenic amine in the dried shrimps as claimed in claim 1, characterized in that: the solid catalyst gradually extends into the soaking solution through a telescopic structure arranged in the container.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110416167.6A CN113109478A (en) | 2021-04-15 | 2021-04-15 | Method for detecting biogenic amine content in dried shrimps |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110416167.6A CN113109478A (en) | 2021-04-15 | 2021-04-15 | Method for detecting biogenic amine content in dried shrimps |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113109478A true CN113109478A (en) | 2021-07-13 |
Family
ID=76718815
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110416167.6A Pending CN113109478A (en) | 2021-04-15 | 2021-04-15 | Method for detecting biogenic amine content in dried shrimps |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113109478A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101793881A (en) * | 2009-12-17 | 2010-08-04 | 东北农业大学 | Method for detecting biogenic amine in food |
| CN102507470A (en) * | 2011-10-20 | 2012-06-20 | 重庆医科大学 | Method for measuring enzyme substrate amount by combining enzyme reaction process method and terminal balance method |
| CN105158243A (en) * | 2015-08-28 | 2015-12-16 | 江苏大学 | Embedded technology-based olfaction visualization auto-detection device and detection method thereof |
| CN111504986A (en) * | 2020-04-03 | 2020-08-07 | 上海理工大学 | Method for rapidly detecting diamine biogenic amine |
| CN211253863U (en) * | 2019-05-28 | 2020-08-14 | 重庆十全环保工程有限公司 | Kitchen garbage microbiological treatment device |
-
2021
- 2021-04-15 CN CN202110416167.6A patent/CN113109478A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101793881A (en) * | 2009-12-17 | 2010-08-04 | 东北农业大学 | Method for detecting biogenic amine in food |
| CN102507470A (en) * | 2011-10-20 | 2012-06-20 | 重庆医科大学 | Method for measuring enzyme substrate amount by combining enzyme reaction process method and terminal balance method |
| CN105158243A (en) * | 2015-08-28 | 2015-12-16 | 江苏大学 | Embedded technology-based olfaction visualization auto-detection device and detection method thereof |
| CN211253863U (en) * | 2019-05-28 | 2020-08-14 | 重庆十全环保工程有限公司 | Kitchen garbage microbiological treatment device |
| CN111504986A (en) * | 2020-04-03 | 2020-08-07 | 上海理工大学 | Method for rapidly detecting diamine biogenic amine |
Non-Patent Citations (2)
| Title |
|---|
| 刘海燕 等: "《物理化学实验》", 31 August 2018, 西安电子科技大学出版社 * |
| 周天政 等: "丹磺酰氯柱前衍生-HPLC法测定鱼粉中的组胺含量", 《黑龙江畜牧兽医》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Guilbault et al. | An improved urea specific enzyme electrode | |
| US5177012A (en) | Biosensor containing immobilized Zymomonas mobilis cells for measuring glucose, fructose and sucrose | |
| CN103063726B (en) | Detection method for chlorine ion content in lithium-ion battery electrolyte | |
| CN104062330A (en) | Electrochemical luminous biosensor for detecting thrombin and manufacture method of biosensor | |
| CN101711356A (en) | Amperometric biosensor for histamine determination | |
| CN105449297B (en) | The quantitative analysis method of electrolyte consumption | |
| Peel | Ester formation by yeasts. 2. Formation of ethyl acetate by washed suspensions of Hansenula anomala | |
| CN105738452B (en) | Method and device for detecting ammonia nitrogen by using all-solid-state ammonia nitrogen sensor | |
| CN113109478A (en) | Method for detecting biogenic amine content in dried shrimps | |
| Okuma et al. | Development of a system with double enzyme reactors for the determination of fish freshness | |
| CN102654475B (en) | Bioelectrochemical sensor used for detecting hydrogen peroxide and manufacturing method thereof | |
| CN114478317B (en) | Ozone fluorescent probe and preparation method and application thereof | |
| Miyauchi et al. | Determination of urea in serum based on the combination of an enzymatic reaction with immobilized urease and ion chromatographic analysis | |
| CN110823974B (en) | Method for detecting formaldehyde by combining p-hydroxybenzaldehyde derivative probe and zinc indium sulfide/zinc oxide | |
| CN114957684A (en) | TCPP (Ni) -Co electrode material and preparation method thereof | |
| CA1260538A (en) | Biochemical detector | |
| CN113866311A (en) | Method for testing content and consumption of electrolyte in battery | |
| Kirstein et al. | Enzyme electrode for urea with amperometric indication: electrode with diffusional limitation | |
| CN112857515A (en) | Liquid level detection method and device, storage medium and kettle | |
| Hasebe et al. | Determinations of citric acid by differential pulse polarography with immobilized enzymes | |
| CN110749637A (en) | CO electrochemical gas sensor based on semi-solid electrolyte and preparation method thereof | |
| CN115465963B (en) | Method for treating waste ointment of rubber and plastic foaming material by-product | |
| Filipović-Kovačević et al. | Cyanide determination in fruit brandies by an amperometric biosensor with immobilised Saccharomyces cerevisiae | |
| CN213875519U (en) | Sodium ion analysis system | |
| Pradáč et al. | Cyclic-voltammetric determination of cysteine in rat organs after intravenous injection |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210713 |
|
| RJ01 | Rejection of invention patent application after publication |