CN113092445A - Method for detecting illegal addition of omeprazole in weight-losing health-care food - Google Patents
Method for detecting illegal addition of omeprazole in weight-losing health-care food Download PDFInfo
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- CN113092445A CN113092445A CN202110398169.7A CN202110398169A CN113092445A CN 113092445 A CN113092445 A CN 113092445A CN 202110398169 A CN202110398169 A CN 202110398169A CN 113092445 A CN113092445 A CN 113092445A
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- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
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Abstract
The invention discloses a method for detecting illegal addition of omeprazole in weight-losing health-care food, which comprises the following steps: weighing 0.2-1.0 g of a weight-reducing health-care product sample, adding 1.3-5.5 mL of an extraction reagent, performing ultrasonic extraction for 8-20 minutes, centrifuging after extraction is finished at a centrifugation speed of 10000 rpm for 2 minutes, and transferring 0.5mL of supernatant into a centrifuge tube after centrifugation; adding 1-5 mL of sodium chloride solution into the supernatant in the centrifuge tube, shaking, uniformly mixing, and standing to serve as a to-be-detected liquid sample for later use; and thirdly, taking 200-1500 muL of gold sol reagent and 8-50 muL of the liquid sample to be detected, uniformly mixing, and then placing in a detection bin of a Raman spectrometer for scanning detection. Through the mode, the invention provides the method for detecting the illegal addition of the omeprazole into the weight-reducing health-care food, so that the detection of the omeprazole in the weight-reducing health-care food can be realized, the method is suitable for on-site rapid detection, and the professional requirements of operators are obviously reduced.
Description
Technical Field
The invention relates to the field of food safety detection, in particular to a method for detecting illegal addition of omeprazole into weight-losing health-care food.
Background
GB16740-2014 published in 2014 of China, namely the Standard for inspection of food (health food) Annationwide, item 2.1 defines the health food as follows: food claimed to have specific health-care function or for supplementing vitamins and minerals. The health food is suitable for specific people, has the function of regulating organism, does not aim at treating diseases, and does not produce any acute, subacute or chronic harm to human body.
In recent years, with the rapid development of equipment economy, the physical living conditions of people are continuously improved, more and more people are obese in China, some obese patients worry about the side effect caused by drug treatment, and the selection of weight-reducing health-care food is safer.
The thirty-eighth article of the food safety law of the people's republic of China also specifies that: "No medicine should be added to the food in production and management". Because the weight-losing health-care food has the characteristics of slow response, no toxic or side effect and the like, some illegal manufacturers illegally add chemical medicines into the weight-losing health-care food to increase the weight-losing efficacy by utilizing the psychology of the consumers in urgent need of losing weight and the propaganda of the health-care food without toxic or side effect.
The existing national standard detection method detects a small number of types of medicines and cannot meet the actual supervision requirement. The main method is to adopt high performance liquid chromatography or high performance liquid chromatography-tandem mass spectrometry to detect the illegal addition of chemicals in the weight-reducing health-care food. The method has expensive detection equipment, high requirement on operators, high instrument detection cost and relatively long detection speed. However, the existing quick detection method mostly adopts a chemical color development method, and the detection sensitivity of the method is low.
At present, most of the existing detection technologies for forbidden additives in health care products adopt liquid chromatography and liquid chromatography tandem mass spectrometry, and the detection technologies have the advantages of wide application range, good separation performance and high sensitivity. But the operation is more complicated, and the equipment volume is bigger, so the field use can not be realized.
Disclosure of Invention
The invention mainly solves the technical problem of providing a method for detecting the illegal addition of omeprazole in weight-reducing health-care food, can realize the detection of omeprazole in the weight-reducing health-care food, is suitable for on-site rapid detection, and obviously reduces the professional requirements of operators.
In order to solve the technical problems, the invention adopts a technical scheme that: the method for detecting the illegal addition of omeprazole in the weight-losing health-care food comprises the following steps:
weighing 0.2-1.0 g of a weight-reducing health-care product sample, adding 1.3-5.5 mL of an extraction reagent, performing ultrasonic extraction for 8-20 minutes, centrifuging after extraction is finished at a centrifugation speed of 10000 rpm for 2 minutes, and transferring 0.5mL of supernatant into a centrifuge tube after centrifugation;
adding 1-5 mL of sodium chloride solution into the supernatant in the centrifuge tube, shaking, uniformly mixing, and standing to serve as a to-be-detected liquid sample for later use;
and thirdly, taking 200-1500 muL of gold sol reagent and 8-50 muL of the liquid sample to be detected, uniformly mixing, and then placing in a detection bin of a Raman spectrometer for scanning detection.
In a preferred embodiment of the invention, the extraction reagent is a 7:3 mixture of methanol and ethanol.
In a preferred embodiment of the present invention, the concentration of the sodium chloride solution is 1.6-4.5 mol/L.
The invention has the beneficial effects that: the method for detecting the illegal addition of omeprazole into the weight-reducing health-care food can realize the detection of omeprazole in the weight-reducing health-care food, is suitable for on-site rapid detection, and obviously reduces the professional requirements of operators.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the description of the embodiments are briefly introduced below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without inventive efforts, wherein:
fig. 1 is an illustration of experimental results of a preferred embodiment of the method for detecting the illegal addition of omeprazole to weight-loss health food according to the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As shown in fig. 1, an embodiment of the present invention includes:
a method for detecting illegally added omeprazole in weight-losing health-care food comprises the following steps:
weighing 0.3g of a weight-losing health-care product sample, adding 3 mL of an extraction reagent, carrying out ultrasonic extraction for 10 minutes, centrifuging after extraction is finished, wherein the centrifugation speed is 10000 r/min, the centrifugation time is 2 minutes, and transferring 0.5mL of supernatant into a centrifuge tube after centrifugation;
adding 1mL of sodium chloride solution into the supernatant, shaking, uniformly mixing, and standing to serve as a solution to be detected for later use;
and step three, taking 500 mu L of gold sol reagent and 20 mu L of sample to be detected, uniformly mixing, and then placing in a detection bin of a Raman spectrometer for scanning detection.
Wherein the extraction reagent is a 7:3 mixture of methanol and ethanol.
Further, the concentration of the sodium chloride solution is 2 mol/L.
Wherein the preparation process of the gold sol reagent comprises the following steps:
adding 100mL of ultrapure water into a 250mL three-neck round-bottom flask, quickly stirring and boiling, adding 1.2mL of 1% chloroauric acid aqueous solution, continuously heating to boil again, adding 1mL of 1% trisodium citrate aqueous solution, keeping stirring for about 25min, finishing the reaction, naturally cooling to room temperature, and sealing with a brown bottle for storage.
The Raman spectrum detection result shown in figure 1 is finally obtained through the processes, and the food safety can be rapidly judged according to the detection result.
In conclusion, the invention provides a method for detecting the illegal addition of omeprazole into weight-reducing health-care food, which can realize the detection of omeprazole in weight-reducing health-care food, is suitable for on-site rapid detection and obviously reduces the professional requirements of operators.
The above description is only an embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes, which are made by the present specification, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.
Claims (3)
1. A method for detecting illegally added omeprazole in weight-losing health-care food is characterized by comprising the following steps:
weighing 0.2-1.0 g of a weight-reducing health-care product sample, adding 1.3-5.5 mL of an extraction reagent, performing ultrasonic extraction for 8-20 minutes, centrifuging after extraction is finished at a centrifugation speed of 10000 rpm for 2 minutes, and transferring 0.5mL of supernatant into a centrifuge tube after centrifugation;
adding 1-5 mL of sodium chloride solution into the supernatant in the centrifuge tube, shaking, uniformly mixing, and standing to serve as a to-be-detected liquid sample for later use;
and thirdly, taking 200-1500 muL of gold sol reagent and 8-50 muL of the liquid sample to be detected, uniformly mixing, and then placing in a detection bin of a Raman spectrometer for scanning detection.
2. The method for detecting the illegal addition of omeprazole into weight-loss health-care food as claimed in claim 1, wherein the extraction reagent is a 7:3 mixture of methanol and ethanol.
3. The method for detecting the illegal addition of omeprazole into weight-losing health-care food according to claim 1, wherein the concentration of the sodium chloride solution is 1.6-4.5 mol/L.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202110398169.7A CN113092445A (en) | 2021-04-14 | 2021-04-14 | Method for detecting illegal addition of omeprazole in weight-losing health-care food |
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| CN202110398169.7A CN113092445A (en) | 2021-04-14 | 2021-04-14 | Method for detecting illegal addition of omeprazole in weight-losing health-care food |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113607716A (en) * | 2021-08-05 | 2021-11-05 | 中朗正健(苏州)生物技术有限公司 | Detection method of illegal food additive |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1379670A (en) * | 1999-08-26 | 2002-11-13 | 艾伊法马公司 | FT-raman spectroscopic measurement of omeprazole isomer ratio in composition |
| CN104697976A (en) * | 2015-03-20 | 2015-06-10 | 河北省食品检验研究院 | Quick extraction and detection method of diethyl yellow in dried bean curd |
| CN110333214A (en) * | 2018-11-14 | 2019-10-15 | 厦门市普识纳米科技有限公司 | The detection method of illegal addition drug ingredient in a kind of health care product |
| CN111380855A (en) * | 2018-12-29 | 2020-07-07 | 同方威视技术股份有限公司 | Method for screening illegal drugs in health care products on site |
-
2021
- 2021-04-14 CN CN202110398169.7A patent/CN113092445A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1379670A (en) * | 1999-08-26 | 2002-11-13 | 艾伊法马公司 | FT-raman spectroscopic measurement of omeprazole isomer ratio in composition |
| CN104697976A (en) * | 2015-03-20 | 2015-06-10 | 河北省食品检验研究院 | Quick extraction and detection method of diethyl yellow in dried bean curd |
| CN110333214A (en) * | 2018-11-14 | 2019-10-15 | 厦门市普识纳米科技有限公司 | The detection method of illegal addition drug ingredient in a kind of health care product |
| CN111380855A (en) * | 2018-12-29 | 2020-07-07 | 同方威视技术股份有限公司 | Method for screening illegal drugs in health care products on site |
Non-Patent Citations (2)
| Title |
|---|
| GAETANO F. BELLIA ET AL.: "Analysis of omeprazole and esomeprazole obtained from traditional pharmacies and unlicensed internet websites using Raman spectroscopy,1H-NMR and chemometric analysis", 《VIBRATIONAL SPECTROSCOPY》 * |
| 冯睿等: "UHPLC-QQQ-MS法测定食品中 30 种非法添加调节消化功能类化合物", 《上海预防医学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113607716A (en) * | 2021-08-05 | 2021-11-05 | 中朗正健(苏州)生物技术有限公司 | Detection method of illegal food additive |
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Application publication date: 20210709 |