CN113088538A - Expression vector based on chimpanzee ChAd3 adenovirus and construction method thereof - Google Patents

Abstract

The invention relates to an expression vector based on chimpanzee ChAd3 adenovirus and a construction method thereof. In one aspect, the invention provides a method of constructing an expression vector based on a chimpanzee ChAd 3-type adenovirus, the method comprising functionally deleting the E1, E3 and E4 regions of the wild-type chimpanzee ChAd 3-type adenovirus whole genome sequence, i.e., the sequence shown in SEQ ID NO: 1. In another aspect, the invention provides an expression vector based on an adenovirus of chimpanzee ChAd3 type, constructed according to the methods described above.

Description

Expression vector based on chimpanzee ChAd3 adenovirus and construction method thereof

Technical Field

The invention belongs to the field of biotechnology and virology; more specifically, the invention relates to an expression vector based on chimpanzee ChAd3 type adenovirus and a construction method thereof.

Background

Adenoviruses belong to the family adenoviridae, and are non-enveloped double-stranded DNA viruses of medium size (diameter 70-90 nm). In the fifties of the last century, adenoviruses were first isolated in human glands (Rowe et al 1953). During the forthcoming forty years, adenoviral vectors have been widely used in gene therapy, oncolytic virus and vaccine development (Chen et al, 2016). By 2019, four Adenovirus-related products were marketed globally with a co-approval, including an attenuated Oral Vaccine against Adenovirus Type 4 and Type 7 infections (Adenoviral Type 4 and Type 7 Vaccine, Live, Oral, USA), recombinant human Adenovirus Type 5 injection (echoy), recombinant human p53 Adenovirus injection (today's recurrences), and recombinant Ebola virus Adenovirus vector Vaccine (Pearson et al 2004, Wu et al 2017). In addition, there are over 200 adenovirus vector-related clinical trials in progress globally. Adenovirus-based medical products have played an increasingly important role in the fields of gene therapy, tumor therapy and vaccine development, and the development of adenovirus vector-based products has important significance.

The adenovirus has genome of about 30-40kb in size, encodes 30-40 genes, and is divided into early gene and late gene. More than one hundred adenovirus serotypes have been isolated and identified (Alonso-Padella et al, 2016). Viral vectors developed based on adenovirus have the following advantages: 1) after the adenovirus infects the cell, the genome can enter the nucleus, so the exogenous antigen of the adenovirus vector can stimulate stronger CD8+ T cell reaction and antibody reaction through an endogenous protein processing way; 2) the modified replication-defective adenovirus has good safety; 3) the adenovirus genome does not integrate into the host genome; 4) adenoviruses are invasive to many types of cells; 4) the adenovirus vector can load exogenous genes with the maximum length of 8 kb; 5) the timeliness of the adenovirus vector for expressing the foreign gene in vivo is longer; 6) the amplification and purification process of the adenovirus is mature, and the virus particles with higher titer can be obtained (Vannucci et al, 2013).

The most used adenovirus vector type in clinical trials was human adenovirus type 5 (HuAd5), which includes three adenovirus-related products approved for marketing in china, and HuAd5 was also used as the vector. However, in 40-60% of humans, neutralizing antibodies against viral vectors have been present in vivo due to their once natural infection with HuAd5, and pre-existing antibodies will greatly inhibit the efficiency of expression of foreign genes and increase vector-mediated cytotoxicity (Xiang et al 2002, perczzi et al 2009). At present, the method is generally adopted to modify rare human adenovirus serotypes (26, 35 and the like) or use non-human primate adenovirus serotypes as vaccine vectors so as to overcome the influence of pre-existing immunity in the population on the effect of vaccine products. For example, since the human body does not contain neutralizing antibodies against chimpanzee adenoviruses, the effect of developing products based on chimpanzee adenoviruses as expression vectors may be significantly better than that of human serum-type adenovirus vectors. Moreover, more and more products developed based on chimpanzee adenovirus vectors also show excellent safety in clinical practice.

There are two methods for constructing recombinant adenovirus vectors: homologous recombination and direct cloning. Homologous recombination methods generally require at least two plasmids, one shuttle plasmid carrying the gene of interest and the other backbone plasmid containing the genomic sequences required for viral assembly. The two plasmids are subjected to homologous recombination in eukaryotic cells or escherichia coli through an inverted repeat sequence or a specific recombinase system to obtain a recombinant virus or recombinant adenovirus vector, such as an AdMax system or an AdEasy system. The method has the disadvantages of low recombination efficiency, easy pollution by parental viruses and complicated steps. Although commercial vector systems based on human HuAd5 type can be used more conveniently at present, on one hand, many product developments cannot be carried out due to patent limitations, and since many novel adenoviruses do not yet establish commercial backbone vectors, it is very time-consuming and labor-consuming (Miravet et al 2014). The direct cloning method is to modify and clone the wild adenovirus genome onto a plasmid vector by a molecular cloning means, and then to transfect to a cell line by linearization to save viruses. The disadvantage of this method is that molecular cloning presents considerable difficulties due to the large genome of the adenovirus (about 36kb) (Zhou et al 2010, Yang et al 2016). Moreover, since wild-type adenovirus is often difficult to obtain (e.g., unavailable for purchase, difficult to isolate, etc.), the use of this method is also greatly limited.

In contrast, the methods described herein advantageously solve the above problems encountered with the current construction of recombinant adenoviral vectors. The method has the advantages of simplicity, accuracy, controllable quality, controllable cost, shorter time, no mutation risk of the sequence, no need of obtaining wild strains in advance, universal applicability and the like.

Disclosure of Invention

The invention aims to provide an expression vector based on chimpanzee ChAd3 adenovirus and a construction method thereof.

In a first aspect of the invention, there is provided a method of constructing an expression vector based on a chimpanzee ChAd3 type adenovirus, the method comprising functionally deleting the E1, E3 and E4 regions of the wild type chimpanzee ChAd3 type adenovirus whole genome sequence (GenBank, # CS479276.1), i.e. the sequence shown in SEQ ID NO: 1.

In a specific embodiment, the nucleotide corresponding to position 589-3252 of the sequence shown in SEQ ID NO. 1 in the E1 region, i.e., the sequence shown in SEQ ID NO. 3, is deleted; the nucleotide corresponding to positions 29125 and 32633 of the sequence shown in SEQ ID NO. 1 in the E3 region, namely the sequence shown in SEQ ID NO. 4 is deleted; and the E4 ORF6 expression cassette sequence in the E4 region corresponding to position 34634 and 37349 of the sequence shown in SEQ ID NO:1, i.e., the sequence shown in SEQ ID NO: 5, was deleted and replaced with the whole genome sequence of chimpanzee adenovirus ChAd68 type shown in SEQ ID NO: 2. Preferably, the expression cassette sequence of the E4 ORF6 is the sequence shown in SEQ ID NO. 8.

In a further embodiment, a sequence containing a cleavage site is inserted into the deleted E1 region. Preferably, the sequence containing the enzyme cutting site comprises one or more sequences selected fromAsiSI、I-CeuI、PI-SceIThe sequence of the cleavage site of (a). More preferably, the sequence containing the enzyme cutting site is a sequence shown in SEQ ID NO. 6.

Alternatively or additionally, a sequence containing a cleavage site is inserted into the deleted E3 region. Preferably, the sequence containing the enzyme cutting site comprisesHpaIAnd/orPacIThe sequence of the cleavage site of (a). More preferably, the sequence containing the enzyme cutting site is a sequence shown as SEQ ID NO. 7.

In further embodiments, the exogenous gene is inserted into one or more of the deleted E1, E3, or E4 regions. Preferably, the foreign gene is inserted into the deleted E1 region. The exogenous gene may be any gene, and may be the same or different. Preferably, the exogenous gene is EGFP.

In a further embodiment, the method further comprises adding a linearization enzyme site, and inserting into the prokaryotic replication plasmid. Preferably, the linearized cleavage site isSwaIAnd (4) enzyme cutting sites. Preferably, the sequence of the prokaryotic replication plasmid is shown as SEQ ID NO 9.

In a second aspect of the invention, there is provided a chimpanzee ChAd 3-based adenovirus expression vector constructed according to the method of any one of the preceding embodiments.

In one embodiment, the expression vector comprises a modified sequence as set forth in SEQ ID NO. 1, wherein the E1, E3, and E4 regions are functionally deleted.

In a specific embodiment, the nucleotide corresponding to position 589-3252 of the sequence shown in SEQ ID NO. 1 in the E1 region, i.e.the sequence shown in SEQ ID NO. 3, is deleted; the nucleotide corresponding to positions 29125-32633 of the sequence shown in SEQ ID NO. 1 in the E3 region, i.e., the sequence shown in SEQ ID NO. 4 was deleted; and the nucleotide corresponding to position 34634-37349 of the sequence shown in SEQ ID NO. 1 in the E4 region, i.e., the E4 ORF6 expression cassette sequence in which the sequence shown in SEQ ID NO. 5 has been deleted and replaced with the whole genome sequence of chimpanzee adenovirus ChAd68 shown in SEQ ID NO. 2. Preferably, the expression cassette sequence of the E4 ORF6 is the sequence shown in SEQ ID NO. 8.

In a further embodiment, the expression vector further comprises a sequence comprising a cleavage site.

In one embodiment, a sequence containing a cleavage site is inserted into the deleted E1 region. Preferably, the sequence containing the enzyme cutting site comprises one or more sequences selected fromAsiSI、I-CeuI、PI-SceIThe sequence of the cleavage site of (a). More preferably, the sequence containing the enzyme cutting site is a sequence shown in SEQ ID NO. 6.

Alternatively or additionally, a sequence containing a cleavage site is inserted into the deleted E3 region. Preferably, the sequence containing the enzyme cutting site comprisesHpaIAnd/orPacIThe sequence of the cleavage site of (a). More preferably, the sequence containing the enzyme cutting site is a sequence shown as SEQ ID NO. 7.

In a further embodiment, the vector further comprises a linearization enzyme site and a prokaryotic replication plasmid. Preferably, the linearized cleavage site isSwaIAnd (4) enzyme cutting sites. Preferably, the sequence of the prokaryotic replication plasmid is shown as SEQ ID NO 9.

In one embodiment, the expression vector comprises the sequence shown in SEQ ID NO. 11. In another embodiment, the sequence of the expression vector is set forth in SEQ ID NO 11.

In a further embodiment, the expression vector may further comprise an exogenous gene.

In one embodiment, the foreign gene may be inserted into one or more of the deleted E1, E3, or E4 regions. Preferably, the foreign gene is inserted into the deleted E1 region. The exogenous gene may be any gene, and may be the same or different. Preferably, the exogenous gene is EGFP. Preferably, the sequence of the expression vector containing the foreign gene is shown as SEQ ID NO. 12.

In a third aspect of the invention, there is provided a host cell comprising an expression vector according to any one of the preceding embodiments.

In one embodiment, the host cell is a eukaryotic cell. Preferably, the eukaryotic cell is a HEK293 cell.

In a fourth aspect of the invention, a method for packaging and amplifying a recombinant adenovirus using a host cell is provided. Optionally, the harvested virus stock is purified. In one embodiment, the host cell is a eukaryotic cell. Preferably, the eukaryotic cell is a HEK293 cell.

Other aspects of the invention will be apparent to those skilled in the art in view of the disclosure herein.

Drawings

FIG. 1: wild type ChAd3 adenovirus genome map and recombinant chimpanzee adenovirus vector plasmid pChAd3-EGFP map carrying reporter gene. A. Wild type adenovirus ChAd3 genome map. Δ E1, Δ E3, Δ E4 represent three deleted regions in the genome, respectively. B. The recombinant chimpanzee adenovirus vector plasmid pChAD3-EGFP sequence map carrying a single reporter gene EGFP.

FIG. 2: the enzyme digestion map verifies the complete sequence of the recombinant chimpanzee adenovirus vector plasmid pChAD3-EGFP carrying the reporter gene.

FIG. 3: typical cytopathic phenomenon appeared after HEK293 cells were infected by recombinant chimpanzee adenovirus ChAd3-EGFP carrying reporter genes.

FIG. 4: and (3) detecting the expression of the fluorescent reporter gene inserted after the recombinant chimpanzee adenovirus ChAd3-EGFP carrying the reporter gene infects HEK293 cells.

FIG. 5: and (3) verifying the whole genome sequence of the purified recombinant adenovirus ChAd3-EGFP by enzyme digestion mapping.

DESCRIPTION OF THE SEQUENCES

1, SEQ ID NO: wild type chimpanzee ChAd3 type adenovirus full sequence (wt-ChAd 3);

2, SEQ ID NO: wild type chimpanzee adenovirus ChAd68 type complete sequence;

3, SEQ ID NO: deleted E1 region sequence;

4, SEQ ID NO: deleted E3 region sequence;

5, SEQ ID NO: deleted E4 region sequence;

6 of SEQ ID NO: a sequence containing a cleavage site inserted in the deleted E1 region;

7, SEQ ID NO: a sequence containing a cleavage site inserted in the deleted E3 region;

8, SEQ ID NO: a ChAd 68E 4 ORF6 sequence inserted in the deleted E4 region;

9 of SEQ ID NO: modified prokaryotic replication plasmid empty vector sequence;

10, SEQ ID NO: two of the deleted E1 areasAsiSAn EGFP-containing exogenous reporter gene coding expression reading frame sequence is inserted between I;

11, SEQ ID NO: recombinant chimpanzee ChAd3 type adenovirus empty vector plasmid sequence;

12, SEQ ID NO: chimpanzee ChAd3 type adenovirus vector plasmid sequence (pChAd3-EGFP) for recombinant expression of EGFP.

Detailed Description

Through a series of intensive researches, the inventor designs a simple and effective strategy, and successfully constructs a novel expression vector based on the prior published wild-type sequence of the chimpanzee ChAd3 adenovirus. The adenovirus expression vector can be applied to the preparation of products such as preventive vaccines, therapeutic vaccines, oncolytic viruses or gene therapy.

The invention not only solves the problem that wild virus strains can not be obtained, but also has simpler and more direct design scheme, can have complete quality control, greatly avoids sequence mutation in the cloning construction process, and can verify the packaging of the virus and the expression of exogenous genes in the shortest time so as to verify the success of vector construction.

In a first aspect of the invention, there is provided a method of constructing an expression vector based on a chimpanzee ChAd3 type adenovirus, the method comprising functionally deleting the E1, E3 and E4 regions of the wild type chimpanzee ChAd3 type adenovirus whole genome sequence (GenBank, # CS479276.1), i.e. the sequence shown in SEQ ID NO: 1.

The term "functional deletion" refers to a modification that has the effect of rendering a particular gene product non-functional, thereby producing a replication-defective recombinant chimpanzee ChAd 3-type adenovirus expression vector. More specifically, it is preferable to delete most of the E1 region, most of the E3 region, and replace most of the E4 region.

In a specific embodiment, the nucleotide corresponding to position 589-3252 of the sequence shown in SEQ ID NO. 1 in the E1 region, i.e., the sequence shown in SEQ ID NO. 3, is deleted; the nucleotide corresponding to positions 29125 and 32633 of the sequence shown in SEQ ID NO. 1 in the E3 region, namely the sequence shown in SEQ ID NO. 4 is deleted; and the E4 ORF6 expression cassette sequence in the E4 region corresponding to position 34634 and 37349 of the sequence shown in SEQ ID NO:1, i.e., the sequence shown in SEQ ID NO: 5, was deleted and replaced with the whole genome sequence of chimpanzee adenovirus ChAd68 type shown in SEQ ID NO: 2. Preferably, the expression cassette sequence of the E4 ORF6 is the sequence shown in SEQ ID NO. 8.

Aiming at the wild type complete sequence of the chimpanzee ChAd3 type adenovirus, the inventor carefully researches and discloses published literature data, in order to ensure that the reconstructed replication-defective recombinant chimpanzee ChAd3 type adenovirus expression vector obtains larger length space of an insert fragment, most of E4 region is deleted, only an ORF6 reading expression frame necessary for virus replication is reserved, and the coding frame is replaced by an expression frame sequence of chimpanzee ChAd68 type adenovirus E4 ORF6, thus being beneficial to obtaining vector adenovirus with stronger replication capacity.

In a further embodiment, a sequence containing a cleavage site is inserted into the deleted E1 region. Preferably, the sequence containing the enzyme cutting site comprises one or more sequences selected fromAsiSI、I-CeuI、PI-SceIThe sequence of the cleavage site of (a). More preferably, the sequence containing the enzyme cutting site is a sequence shown in SEQ ID NO. 6.

Alternatively or additionally, a sequence containing a cleavage site is inserted into the deleted E3 region. Preferably, the sequence containing the enzyme cutting site comprisesHpaIAnd/orPacIThe sequence of the cleavage site of (a). More preferably, the sequence containing the enzyme cutting site is a sequence shown as SEQ ID NO. 7.

Aiming at the wild complete sequence of chimpanzee ChAd3 type adenovirus, the inventor finally determines the application enzyme cutting site through careful sequence alignmentAsiSI、I-CeuI、PI-SceIAs the insertion site of the exogenous gene in the E1 deletion region, the method can not cause error shearing at other positions of the adenovirus expression vector, and the selection of multiple sites is also beneficial to subsequent more convenient selection. At the same time, the user can select the desired position,Hpai andPaci as insertion site of exogenous gene in E3 deletion regionAlso, the method does not cause error shearing, and provides a convenient insertion site for inserting the exogenous gene in the E3 region.

In further embodiments, the exogenous gene is inserted into one or more of the deleted E1, E3, or E4 regions. Preferably, the foreign gene is inserted into the deleted E1 region. The exogenous gene may be any gene, and may be the same or different. Preferably, the exogenous gene is EGFP.

In a further embodiment, the method further comprises adding a linearization enzyme site, and inserting into the prokaryotic replication plasmid. Preferably, the linearized cleavage site isSwaIThe restriction enzyme site and the recognition sequence are as follows: atttaaaat. The site can not cause the wrong shearing of the sequence, and the sheared site is a flat end, which is more favorable for the stability of the adenovirus after the whole sequence linearization.

Preferably, the sequence of the prokaryotic replication plasmid is shown as SEQ ID NO 9. More specifically, the prokaryotic replication plasmid is transformed from pcDNA3.1 plasmid and comprises a basic replication initiation site, an ampicillin resistance selection gene and a single enzyme cutting siteSwaITo facilitate the insertion of the adenoviral vector sequence, as shown in SEQ ID NO 9. The present inventors have deleted most of the pcDNA3.1 sequence, such as a large number of cleavage site sequences, by detailed sequence alignment, so as to avoid the occurrence of the erroneous cleavage by the endonuclease. Only the most central replication origin sequence and the expression cassette sequence of the resistance selection gene of the plasmid are reserved for the subsequent amplification and selection of the plasmid.

In one embodiment, the method comprises inserting a sequence comprising a specific cleavage site into the deleted E1 region (e.g.AsiSI、I-CeuI、PI-SceI) Region E3 (e.g. region E3)HpaI andPacI) (ii) a The deleted E4 region was replaced with a chimpanzee Adenovirus ChAd68 type (also known as Paniscus Adenovirus 6 or Simian Adenovirus 25, GenBank, # AC _000011.1) (SEQ ID NO: 2) E4 ORF6 expression cassette sequence, preferably the sequence shown in SEQ ID NO: 8; finally, a linearization site is added to the head and tail of the complete sequence (e.g.SwaI) And inserting the plasmid into prokaryotic replication plasmid with the site as boundary to obtain replication-defective recombinant blackThe sequence of the chimpanzee ChAd3 type adenovirus expression vector is shown as SEQ ID NO. 11. The obtained complete sequence is entrusted to professional gene synthesis companies for complete sequence synthesis, and finally plasmid which covers accurate sequence information and sufficient transfection level purification is obtained.

In a second aspect of the invention, there is provided a chimpanzee ChAd 3-based adenovirus expression vector constructed according to the method of any one of the preceding embodiments.

In one embodiment, the expression vector comprises a modified sequence as set forth in SEQ ID NO. 1, wherein the E1, E3, and E4 regions are functionally deleted.

In a specific embodiment, the nucleotide corresponding to position 589-3252 of the sequence shown in SEQ ID NO. 1 in the E1 region, i.e.the sequence shown in SEQ ID NO. 3, is deleted; the nucleotide corresponding to positions 29125-32633 of the sequence shown in SEQ ID NO. 1 in the E3 region, i.e., the sequence shown in SEQ ID NO. 4 was deleted; and the nucleotide corresponding to position 34634-37349 of the sequence shown in SEQ ID NO. 1 in the E4 region, i.e., the E4 ORF6 expression cassette sequence in which the sequence shown in SEQ ID NO. 5 has been deleted and replaced with the whole genome sequence of chimpanzee adenovirus ChAd68 shown in SEQ ID NO. 2. Preferably, the expression cassette sequence of the E4 ORF6 is the sequence shown in SEQ ID NO. 8.

In a further embodiment, the expression vector further comprises a sequence comprising a cleavage site.

In one embodiment, a sequence containing a cleavage site is inserted into the deleted E1 region. Preferably, the sequence containing the enzyme cutting site comprises one or more sequences selected fromAsiSI、I-CeuI、PI-SceIThe sequence of the cleavage site of (a). More preferably, the sequence containing the enzyme cutting site is a sequence shown in SEQ ID NO. 6.

Alternatively or additionally, a sequence containing a cleavage site is inserted into the deleted E3 region. Preferably, the sequence containing the enzyme cutting site comprisesHpaIAnd/orPacIThe sequence of the cleavage site of (a). More preferably, the sequence containing the enzyme cutting site is a sequence shown as SEQ ID NO. 7.

In a further embodiment, the vector further comprises a linearization enzyme site anda prokaryotic replication plasmid. Preferably, the linearized cleavage site isSwaIAnd (4) enzyme cutting sites. Preferably, the sequence of the prokaryotic replication plasmid is shown as SEQ ID NO 9.

In one embodiment, the expression vector comprises the sequence shown in SEQ ID NO. 11. In another embodiment, the sequence of the expression vector is set forth in SEQ ID NO 11.

In further embodiments, the expression vector may further comprise one or more exogenous genes.

In one embodiment, the foreign gene may be inserted into one or more of the deleted E1, E3, or E4 regions. Preferably, a foreign gene is inserted into the deleted E1 region, thereby obtaining an adenovirus expression vector that can express the foreign gene. The exogenous gene may be any gene. Preferably, the exogenous gene is EGFP.

In one embodiment, two of the E1 regions are deletedAsiSIAn exogenous reporter gene coding expression reading frame containing EGFP is inserted between the insertion sites to obtain a recombinant adenovirus vector capable of expressing an exogenous gene, and the sequence of the recombinant adenovirus vector is shown as SEQ ID NO. 12.

Preferably, the expression reading frame coded by the EGFP-containing exogenous reporter gene refers to an EGFP expression reading frame sequence initiated by a CMV promoter, and is shown as SEQ ID NO. 10.

In addition, preferably, after the sequence is completely constructed in sequence analysis software, the recombinant adenovirus vector plasmid pChAD3-EGFP capable of expressing a foreign gene is obtained directly by a whole-gene combination mode.

Specifically, to verify the effectiveness of the constructed adenovirus expression vector in expressing foreign genes, the present inventors inserted the fluorescent reporter gene EGFP into the deleted E1 region. The result shows that the constructed chimpanzee ChAd3 type adenovirus carrying the reporter gene can efficiently express the exogenous gene, and proves that the inventor successfully obtains a novel adenovirus expression vector which can be used for research and development of biological products or basic research of biomedicine.

In a third aspect of the invention, there is provided a host cell comprising an expression vector according to any one of the preceding embodiments.

In one embodiment, the host cell is a eukaryotic cell. Preferably, the eukaryotic cell is a HEK293 cell.

In a fourth aspect of the invention, a method for packaging and amplifying a recombinant adenovirus using a host cell is provided. Preferably, the harvested virus can be repeatedly infected into production cells for continuous passage. Optionally, the harvested virus stock may be purified. The determination of the virus titer can be performed according to conventional methods known in the art.

The adenovirus expression vector is used as a vector platform capable of expressing exogenous genes, is suitable for expressing one, two or even multiple exogenous genes, and is further applied to preparing products such as preventive vaccines, therapeutic vaccines, oncolytic viruses or gene therapy.

The method of the invention has the following outstanding advantages:

A. after the complete sequence is obtained by software sequence analysis, the method successfully avoids the traditional method of constructing the adenovirus vector by homologous recombination and greatly reduces the possibility of the mutation of the adenovirus vector sequence by the direct gene synthesis of the complete sequence of the adenovirus vector plasmid.

B. Compared with the method of directly cloning by a wild adenovirus sequence, the method of the invention has simpler and more direct operation and does not have the defects of a direct cloning method. The direct cloning method usually has a high failure rate of molecular cloning experiments due to the large adenovirus genome sequence (about 36 kb). The molecular cloning usually depends on the splicing of a specific enzyme cutting site and a PCR amplification sequence, and the enzyme cutting site is not easy to find; multiple and long-fragment PCR amplifications increase the mutation potential of the sequences; the molecular cloning failure rate is higher for high GC content sequence.

C. The method of the invention adopts more-expensive industrialized and standardized gene synthesis, and can ensure 100 percent of sequence accuracy, quality control of purified plasmids, cost control of plasmid preparation and time control of vector construction. For ordinary laboratory operators, only simple sequence analysis and alignment need to be mastered.

D. The method of the invention does not depend on the acquisition of wild adenovirus strains, and can be carried out by only adopting the published complete sequence of wild adenovirus genome, thereby ensuring wider and more batch application.

Therefore, the invention not only provides a novel adenovirus expression vector, but also provides a novel adenovirus vector construction method, which is more favorable for promoting the adenovirus vector to exert excellent characteristics in the fields of biomedical basic research and clinical application and development, and lays a certain foundation for the health career of human beings.

Examples

The invention will be further illustrated with reference to the following specific examples. It should be understood that the examples of the present invention are illustrative only and are not intended to limit the scope of the present invention. The experimental procedures, for which conditions are not indicated in the following examples, are generally carried out according to conventional experimental conditions, molecular cloning protocols or conditions recommended by the manufacturers of the relevant reagents (cassettes).

1. Experimental Material

1.1. Cells

The HEK293 cell line was purchased from ATCC cell bank (cat # CRL-1573) in the United states, and the medium was DMEM (GIBCO, #11995-040) containing 10% fetal bovine serum (GIBCO, # 10091-148).

1.2. Restriction enzyme

Restriction enzymes were purchased from New England Biolabs.

2. Experimental methods

2.1. Construction of an adenovirus vector plasmid of chimpanzee ChAd3 type carrying a reporter gene.

In the sequence analysis software (e.g., SnapGene, Vector NTI, etc.), the nucleotide corresponding to position 589-3252 of the sequence shown in SEQ ID NO:1 in region E1, i.e., the sequence shown in SEQ ID NO: 3, is deleted; the nucleotide corresponding to positions 29125 and 32633 of the sequence shown in SEQ ID NO. 1 in the E3 region, namely the sequence shown in SEQ ID NO. 4 is deleted; and the nucleotide corresponding to position 34634 and 37349 of the sequence shown in SEQ ID NO. 1 in the E4 region, i.e., the sequence shown in SEQ ID NO. 5, was deleted and replaced with the sequence shown in SEQ ID NO. 8. Furthermore, two cleavage sites were inserted into the deleted E1 regionAsiSI(ii) a Inserting in deleted E3 areaCleavage siteHpaIAndPacI. Then, the fluorescent reporter EGFP was inserted into the deleted E1 region. Finally, adding linear enzyme cutting sites at the head and tail of the complete sequenceSwaI, and inserting the plasmid into a prokaryotic replication plasmid (the sequence of which is shown as SEQ ID NO: 9) by taking the site as a boundary so as to obtain a replication-defective recombinant chimpanzee ChAd3 type adenovirus expression vector for recombinant expression of EGFP, namely the complete sequence of the plasmid pChAD3-EGFP, which is shown as SEQ ID NO: 12. The obtained complete sequence was assigned to Soviet Kirgiz Biotech, Inc. for complete sequence synthesis to finally obtain the plasmid pChAD3-EGFP, which is purified at transfection level, and is shown in the plasmid map of FIG. 1.

2.2. Identification of plasmid sequence of chimpanzee ChAd3 type adenovirus vector carrying reporter gene

Carrying out primary enzyme digestion map verification on plasmids obtained by gene synthesis, carrying out standardized enzyme digestion with restriction enzymes respectively, and carrying out DNA analysis and identification, wherein the DNA analysis and identification comprisesHindIII & XbaI double enzyme digestion,XhoI & XbaI double enzyme digestion,NotI & XbaI double enzyme digestion,NdeI & XbaI double cleavage andPvui, single enzyme digestion. And comparing the actual enzyme digestion map with the enzyme digestion map predicted by software according to the expected sequence to verify the correctness of the sequence. The results showed that both plasmid sequences obtained were fully expected, see FIG. 2.

2.3. Chimpanzee ChAd3 type adenovirus package carrying reporter gene

Using restriction enzymesSwaI plasmid pChAD3-EGFP was subjected to linearized digestion. After 6-well plates were plated with HEK293 cells and the cell density reached 80% -90%, approximately 6. mu.g of linearized pChAD3-EGFP digest was transfected with the transfection reagent Lipofectamine 2000 (Invitrogen, #11668019), the cells were observed daily for cytopathy (see FIG. 3) and for fluorescent reporter gene expression (see FIG. 4). After about 4-6 days, significant fluorescence expression and cytopathic effects were observed, demonstrating successful viral packaging. The transfected cell product is harvested, frozen and thawed repeatedly for three times, and centrifuged to obtain supernatant, which can be used as initial virus stock solution.

2.4. Chimpanzee ChAd3 type adenovirus amplification carrying reporter gene

HEK293 cells were cultured in DMEM medium containing 10% fetal bovine serum to near 100% cell density, and the cells were continued to be cultured by adding the initial virus stock to the cell culture medium. When the cells have cytopathic effect and almost all the cells are changed from adherent to floating state, all the infected cells can be collected, and the cells are collected by centrifugation. Resuspending the cells with a suitable amount of DMEM, repeatedly freezing and thawing for three times to lyse the cells, and centrifuging to obtain the supernatant. The supernatant can be further amplified repeatedly according to the above method to obtain a certain amount of virus stock solution.

2.5. Purification of chimpanzee ChAd3 adenovirus carrying reporter gene

The amplified virus stock solution was purified and titer-determined by cesium chloride density gradient centrifugation as described in published literature (Zhou et al 2010), and then stored in a refrigerator at-80 ℃ for a long period of time with glycerol added.

2.6. Identification of genome sequence of chimpanzee ChAd3 type adenovirus carrying reporter gene

The titer is taken to be 0.5-1x10¹²Purified virus solution of individual virus particles (vps) using kit DNeasy Blood&Viral genome extraction was performed with Tissue Kit (QIAGEN, # 69504). The extracted viral genome is subjected to standardized digestion by using restriction enzymes and then subjected to DNA analysis and identification, includingHindIII & XbaI double enzyme digestion,XhoI & XbaI double enzyme digestion,NotI & XbaI double enzyme digestion,NdeI & XbaI double cleavage andPvui, single enzyme digestion. And comparing the actual enzyme digestion map with the enzyme digestion map predicted by software according to the expected sequence to verify the correctness of the sequence. The results showed that both plasmid sequences obtained were fully expected, see FIG. 5.

3. Conclusion

By the method for directly synthesizing the adenovirus genome, the inventor constructs the wild chimpanzee ChAd3 adenovirus into a vector capable of expressing exogenous genes. Therefore, the invention provides a chimpanzee ChAd3 type adenovirus vector for the research and development of novel adenovirus vector related products and the basic research of biomedicine thereof, and also provides a novel method for constructing the adenovirus vector from the beginning.

All references mentioned in this application are incorporated by reference in this application as if each were individually incorporated by reference. It should also be understood that various changes or modifications may be made by those skilled in the art after reading the above disclosure of the present application, and such equivalents may fall within the scope of the invention as defined by the appended claims.

Reference to the literature

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5.Alonso-Padilla J, Papp T, Kaján GL, et al., Development of novel adenoviral vectors to overcome challenges observed with HAdV-5–based constructs. Molecular Therapy, 2016, 24(1): p6-16.

6.Vannucci L, Lai M, Chiuppesi F, et al., Viral vectors: a look back and ahead on gene transfer technology. New Microbiologica, 2013, 36(1): p1-22.

7.Xiang Z, Gao G, Reyes-Sandoval A, et al., Novel, chimpanzee serotype 68-based adenoviral vaccine carrier for induction of antibodies to a transgene product. Journal of Virology, 2002, 76(6): p2667-2675.

8.Peruzzi D, Dharmapuri S, Cirillo A, et al., A novel chimpanzee serotype-based adenoviral vector as delivery tool for cancer vaccines. Vaccine, 2009, 27(9): p1293-1300.

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Sequence listing

<110> Shanghai Yidao Biotechnology Co., Ltd

<120> expression vector based on chimpanzee ChAd3 type adenovirus and construction method thereof

<130> CPCH1963152N

<160> 12

<170> PatentIn version 3.5

<210> 1

<211> 37741

<212> DNA

<213> Pan troglodytes

<400> 1

catcatcaat aatatacctt attttggatt gaagccaata tgataatgag atgggcggcg 60

cgaggcgggg cgcggggcgg gaggcgggtt tgggggcggg ccggcgggcg gggcggtgtg 120

gcggaagtgg actttgtaag tgtggcggat gtgacttgct agtgccgggc gcggtaaaag 180

tgacgttttc cgtgcgcgac aacgcccccg ggaagtgaca tttttcccgc ggtttttacc 240

ggatgttgta gtgaatttgg gcgtaaccaa gtaagatttg gccattttcg cgggaaaact 300

gaaacgggga agtgaaatct gattaatttt gcgttagtca taccgcgtaa tatttgtcta 360

gggccgaggg actttggccg attacgtgga ggactcgccc aggtgttttt tgaggtgaat 420

ttccgcgttc cgggtcaaag tctccgtttt attattatag tcagctgacg cggagtgtat 480

ttataccctc tgatctcgtc aagaggccac tcttgagtgc cagcgagtag agttttctcc 540

tctgccgctc tccgctccgc tccgctcggc tctgacaccg gggaaaaaat gagacatttc 600

acctacgatg gcggtgtgct caccggccag ctggctgctg aggtcctgga caccctgatc 660

gaggaggtat tggccgataa ttatcctccc tcgactcctt ttgagccacc tacacttcac 720

gaactatacg atctggatgt ggtggggccc agcgatccga acgagcaggc ggtttccagt 780

ttttttccag agtccatgtt gttggccagc caggaggggg tcgaacttga gacccctcct 840

ccgatcgtgg attcccccga tccgccgcag ctgactaggc agcccgagcg ctgtgcggga 900

cctgagacta tgccccagct gctacctgag gtgatcgatc tcacctgtaa tgagtctggt 960

tttccaccca gcgaggatga ggacgaagag ggtgagcagt ttgtgttaga ttctgtggaa 1020

caacccgggc gaggatgcag gtcttgtcaa tatcaccgga aaaacacagg agactcccag 1080

attatgtgtt ctctgtgtta tatgaagatg acctgtatgt ttatttacag taagtttatc 1140

atcggtgggc aggtgggcta tagtgtgggt ggtggtcttt ggggggtttt ttaatatatg 1200

tcaggggtta tgctgaagac ttttttattg tgatttttaa aggtccagtg tctgagcccg 1260

agcaagaacc tgaaccggag cctgagcctt ctcgccccag gagaaagcct gtaatcttaa 1320

ctagacccag cgcaccggta gcgagaggcc tcagcagcgc ggagaccacc gactccggtg 1380

cttcctcatc acccccggag attcaccccc tggtgcccct atgtcccgtt aagcccgttg 1440

ccgtgagagt cagtgggcgg cggtctgctg tggagtgcat tgaggacttg ctttttgatt 1500

cacaggaacc tttggacttg agcttgaaac gccccaggca ttaaacctgg tcacctggac 1560

tgaatgagtt gacgcctatg tttgcttttg aatgacttaa tgtgtataga taataaagag 1620

tgagataatg ttttaattgc atggtgtgtt taacttgggc ggagtctgct gggtatataa 1680

gcttccctgg gctaaacttg gttacacttg acctcatgga ggcctgggag tgtttggaga 1740

actttgccgg agttcgtgcc ttgctggacg agagctctaa caatacctct tggtggtgga 1800

ggtatttgtg gggctctccc cagggcaagt tagtttgtag aatcaaggag gattacaagt 1860

gggaatttga agagcttttg aaatcctgtg gtgagctatt ggattctttg aatctaggcc 1920

accaggctct cttccaggag aaggtcatca ggactttgga tttttccaca ccggggcgca 1980

ttgcagccgc ggttgctttt ctagcttttt tgaaggatag atggagcgaa gagacccact 2040

tgagttcggg ctacgtcctg gattttctgg ccatgcaact gtggagagca tggatcagac 2100

acaagaacag gctgcaactg ttgtcttccg tccgcccgtt gctgattccg gcggaggagc 2160

aacaggccgg gtcagaggac cgggcccgtc gggatccgga ggagagggca ccgaggccgg 2220

gcgagaggag cgcgctgaac ctgggaaccg ggctgagcgg ccatccacat cgggagtgaa 2280

tgtcgggcag gtggtggatc tttttccaga actgcggcgg attttgacta ttagggagga 2340

tgggcaattt gttaagggtc ttaagaggga gaggggggct tctgagcata acgaggaggc 2400

cagtaattta gcttttagct tgatgaccag acaccgtcca gagtgcatca cttttcagca 2460

gattaaggac aattgtgcca atgagttgga tctgttgggt cagaagtata gcatagagca 2520

gctgaccact tactggctgc agccgggtga tgatctggag gaagctatta gggtgtatgc 2580

taaggtggcc ctgcggcccg attgcaagta caagctcaag gggctggtga atatcaggaa 2640

ttgttgctac atttctggca acggggcgga ggtggagata gagaccgaag acagggtggc 2700

tttcagatgc agcatgatga atatgtggcc gggggtgctg ggcatggacg gggtggtgat 2760

tatgaatgtg aggttcacgg ggcccaactt taacggcacg gtgtttttgg ggaacaccaa 2820

cctggtcctg cacggggtga gcttctatgg gtttaacaac acctgtgtgg aggcctggac 2880

cgatgtgaag gtccgcggtt gcgcctttta tggatgttgg aaggccatag tgagccgccc 2940

taagagcagg agttccatta agaaatgctt gtttgagagg tgcaccttgg ggatcctggc 3000

cgagggcaac tgcagggtgc gccacaatgt ggcctccgag tgcggttgct tcatgctagt 3060

caagagcgtg gcggtaatca agcataatat ggtgtgcggc aacagcgagg acaaggcctc 3120

acagatgctg acctgcacgg atggcaactg ccacttgctg aagaccatcc atgtaaccag 3180

ccacagccgg aaggcctggc ccgtgttcga gcacaacttg ctgacccgct gctccttgca 3240

tctgggcaac aggcgggggg tgttcctgcc ctatcaatgc aactttagtc acaccaagat 3300

cttgctagag cccgagagca tgtccaaggt gaacttgaac ggggtgtttg acatgaccat 3360

gaagatctgg aaggtgctga ggtacgacga gaccaggtcc cggtgcagac cctgcgagtg 3420

cgggggcaag catatgagga accagcccgt gatgctggat gtgaccgagg agctgaggac 3480

agaccacttg gttctggcct gcaccagggc cgagtttggt tctagcgatg aagacacaga 3540

ttgaggtggg tgagtgggcg tggcctgggg tggtcatgaa aatatataag ttgggggtct 3600

tagggtctct ttatttgtgt tgcagagacc gccggagcca tgagcgggag cagcagcagc 3660

agcagtagca gcagcgcctt ggatggcagc atcgtgagcc cttatttgac gacgcggatg 3720

ccccactggg ccggggtgcg tcagaatgtg atgggctcca gcatcgacgg ccgacccgtc 3780

ctgcccgcaa attccgccac gctgacctat gcgaccgtcg cggggacgcc gttggacgcc 3840

accgccgccg ccgccgccac cgcagccgcc tcggccgtgc gcagcctggc cacggacttt 3900

gcattcctgg gaccactggc gacaggggct acttctcggg ccgctgctgc cgccgttcgc 3960

gatgacaagc tgaccgccct gctggcgcag ttggatgcgc ttactcggga actgggtgac 4020

ctttctcagc aggtcatggc cctgcgccag caggtctcct ccctgcaagc tggcgggaat 4080

gcttctccca caaatgccgt ttaagataaa taaaaccaga ctctgtttgg attaaagaaa 4140

agtagcaagt gcattgctct ctttatttca taattttccg cgcgcgatag gccctagacc 4200

agcgttctcg gtcgttgagg gtgcggtgta tcttctccag gacgtggtag aggtggctct 4260

ggacgttgag atacatgggc atgagcccgt cccgggggtg gaggtagcac cactgcagag 4320

cttcatgctc cggggtggtg ttgtagatga tccagtcgta gcaggagcgc tgggcatggt 4380

gcctaaaaat gtccttcagc agcaggccga tggccagggg gaggcccttg gtgtaagtgt 4440

ttacaaaacg gttaagttgg gaagggtgca ttcggggaga gatgatgtgc atcttggact 4500

gtatttttag attggcgatg tttccgccca gatcccttct gggattcatg ttgtgcagga 4560

ccaccagtac agtgtatccg gtgcacttgg ggaatttgtc atgcagctta gagggaaaag 4620

cgtggaagaa cttggagacg cccttgtggc ctcccagatt ttccatgcat tcgtccatga 4680

tgatggcaat gggcccgcgg gaggcagctt gggcaaagat atttctgggg tcgctgacgt 4740

cgtagttgtg ttccagggtg aggtcgtcat aggccatttt tacaaagcgc gggcggaggg 4800

tgcccgactg ggggatgatg gtcccctctg gccctggggc gtagttgccc tcgcagatct 4860

gcatttccca ggccttaatc tcggaggggg gaatcatatc cacctgcggg gcgatgaaga 4920

aaacggtttc cggagccggg gagattaact gggatgagag caggtttcta agcagctgtg 4980

attttccaca accggtgggc ccataaataa cacctataac cggttgcagc tggtagttta 5040

gagagctgca gctgccgtcg tcccggagga ggggggccac ctcgttgagc atgtccctga 5100

cgcgcatgtt ctccccgacc agatccgcca gaaggcgctc gccgcccagg gacagcagct 5160

cttgcaagga agcaaagttt ttcagcggct tgaggccgtc cgccgtgggc atgtttttca 5220

gggtctggct cagcagctcc aggcggtccc agagctcggt gacgtgctct acggcatctc 5280

tatccagcat atctcctcgt ttcgcgggtt ggggcgactt tcgctgtagg gcaccaagcg 5340

gtggtcgtcc agcggggcca aagtcatgtc cttccatggg cgcagggtcc tcgtcagggt 5400

ggtctgggtc acggtgaagg ggtgcgctcc gggctgagcg cttgccaagg tgcgcttgag 5460

gctggttctg ctggtgctga agcgctgccg gtcttcgccc tgcgcgtcgg ccaggtagca 5520

tttgaccatg gtgtcatagt ccagcccctc cgcggcgtgt cccttggcgc gcagcttgcc 5580

cttggaggtg gcgccgcacg aggggcagag caggctcttg agcgcgtaga gcttgggggc 5640

gaggaagacc gattcggggg agtaggcgtc cgcgccgcag accccgcaca cggtctcgca 5700

ctccaccagc caggtgagct cggggcgcgc cgggtcaaaa accaggtttc ccccatgctt 5760

tttgatgcgt ttcttacctc gggtctccat gaggtggtgt ccccgctcgg tgacgaagag 5820

gctgtccgtg tctccgtaga ccgacttgag gggtcttttc tccagggggg tccctcggtc 5880

ttcctcgtag aggaactcgg accactctga gacgaaggcc cgcgtccagg ccaggacgaa 5940

ggaggctatg tgggaggggt agcggtcgtt gtccactagg gggtccacct tctccaaggt 6000

gtgaagacac atgtcgcctt cctcggcgtc caggaaggtg attggcttgt aggtgtaggc 6060

cacgtgaccg ggggttcctg acgggggggt ataaaagggg gtgggggcgc gctcgtcgtc 6120

actctcttcc gcatcgctgt ctgcgagggc cagctgctgg ggtgagtatt ccctctcgaa 6180

ggcgggcatg acctccgcgc tgaggttgtc agtttccaaa aacgaggagg atttgatgtt 6240

cacctgtccc gaggtgatac ctttgagggt acccgcgtcc atctggtcag aaaacacgat 6300

ctttttattg tccagcttgg tggcgaacga cccgtagagg gcgttggaga gcagcttggc 6360

gatggagcgc agggtctggt tcttgtccct gtcggcgcgc tccttggccg cgatgttgag 6420

ctgcacgtac tcgcgcgcga cgcagcgcca ctcggggaag acggtggtgc gctcgtcggg 6480

caccaggcgc acgcgccagc cgcggttgtg cagggtgacc aggtccacgc tggtggcgac 6540

ctcgccgcgc aggcgctcgt tggtccagca gagacggccg cccttgcgcg agcagaaggg 6600

gggcaggggg tcgagctggg tctcgtccgg ggggtccgcg tccacggtga aaaccccggg 6660

gcgcaggcgc gcgtcgaagt agtctatctt gcaaccttgc atgtccagcg cctgctgcca 6720

gtcgcgggcg gcgagcgcgc gctcgtaggg gttgagcggc gggccccagg gcatggggtg 6780

ggtgagtgcg gaggcgtaca tgccgcagat gtcatagacg tagaggggct cccgcaggac 6840

cccgatgtag gtggggtagc agcggccgcc gcggatgctg gcgcgcacgt agtcatacag 6900

ctcgtgcgag ggggcgagga ggtcggggcc caggttggtg cgggcggggc gctccgcgcg 6960

gaagacgatc tgcctgaaga tggcatgcga gttggaagag atggtggggc gctggaagac 7020

gttgaagctg gcgtcctgca ggccgacggc gtcgcgcacg aaggaggcgt aggagtcgcg 7080

cagcttgtgt accagctcgg cggtgacctg cacgtcgagc gcgcagtagt cgagggtctc 7140

gcggatgatg tcatatttag cctgcccctt ctttttccac agctcgcggt tgaggacaaa 7200

ctcttcgcgg tctttccagt actcttggat cgggaaaccg tccggttccg aacggtaaga 7260

gcctagcatg tagaactggt tgacggcctg gtaggcgcag cagcccttct ccacggggag 7320

ggcgtaggcc tgcgcggcct tgcggagcga ggtgtgggtc agggcgaagg tgtccctgac 7380

catgactttg aggtactggt gcttgaagtc ggagtcgtcg cagccgcccc gctcccagag 7440

cgagaagtcg gtgcgcttct tggagcgggg gttgggcaga gcgaaggtga catcgttgaa 7500

gaggattttg cccgcgcggg gcatgaagtt gcgggtgatg cggaagggcc ccggcacttc 7560

agagcggttg ttgatgacct gggcggcgag cacgatctcg tcgaagccgt tgatgttgtg 7620

gcccacgatg tagagttcca ggaagcgggg ccggcccttt acggtgggca gcttctttag 7680

ctcttcgtag gtgagctcct cgggcgaggc gaggccgtgc tcggccaggg cccagtccgc 7740

gaggtgcggg ttgtctctga ggaaggactc ccagaggtcg cgggccagga gggtctgcag 7800

gcggtccctg aaggtcctga actggcggcc cacggccatt ttttcggggg tgatgcagta 7860

gaaggtgagg gggtcttgct gccagcggtc ccagtcgagc tgcagggcga ggtcgcgcgc 7920

ggcggtgacc aggcgctcgt cgcccccgaa tttcatgacc agcatgaagg gcacgagctg 7980

ctttccgaag gcccccatcc aagtgtaggt ctctacatcg taggtgacaa agaggcgctc 8040

cgtgcgagga tgcgagccga tcgggaagaa ctggatctcc cgccaccagt tggaggagtg 8100

gctgttgatg tggtggaagt agaagtcccg tcgccgggcc gaacactcgt gctggctttt 8160

gtaaaagcga gcgcagtact ggcagcgctg cacgggctgt acctcctgca cgagatgcac 8220

ctttcgcccg cgcacgagga agccgagggg aaatctgagc cccccgcctg gctcgcggca 8280

tggctggtgc tcttctactt tggatgcgtg tccgtctccg tctggctcct cgaggggtgt 8340

tacggtggag cggaccacca cgccgcgcga gccgcaggtc cagatatcgg cgcgcggcgg 8400

tcggagtttg atgacgacat cgcgcagctg ggagctgtcc atggtctgga gctcccgcgg 8460

cggcggcagg tcagccggga gttcttgcag gttcacctcg cagagtcggg ccagggcgcg 8520

gggcaggtct aggtggtacc tgatctctag gggcgtgttg gtggcggcgt cgatggcttg 8580

caggagcccg catccccggg gggcgacgac ggtgccccgc ggggtggtgg tggtggtggt 8640

ggtggtggtg gtggcggtgc agctcagaag cggtgccgcg ggcgggcccc cggaggtagg 8700

gggggctccg gtcccgccgg caggggcggc agcggcacgt cggcgtggag cgcgggcagg 8760

agttggtgct gtgcccggag gttgctggcg aaggcgacga cgcggcggtt gatctcctgg 8820

atctggcgcc tctgcgtgaa gacgacgggc ccggtgagct tgaacctgaa agagagttcg 8880

acagaatcaa tctcggtgtc attgaccgcg gcctggcgca ggatctcctg cacgtctccc 8940

gagttgtctt ggtaggcgat ctcggccatg aactgctcga tctcttcctc ctggaggtct 9000

ccgcgtccgg cgcgttccac ggtggccgcc aggtcgttgg agatgcgccc catgagctgc 9060

gagaaggcgt tgagtccgcc ctcgttccag actcggctgt agaccacgcc cccctggtca 9120

tcgcgggcgc gcatgaccac ctgcgcgagg ttgagctcca cgtgccgcgc gaagacggcg 9180

tagttgcgca gacgctggaa gaggtagttg agggtggtgg cggtgtgctc ggccacgaag 9240

aagttcatga cccagcggcg caacgtggat tcgttgatgt cccccaaggc ctccagccgt 9300

tccatggcct cgtagaagtc cacggcgaag ttgaaaaact gggagttgcg cgccgacacg 9360

gtcaactcct cctccagaag acggatgagc tcggcgacgg tgtcgcgcac ctcgcgctcg 9420

aaggctatgg ggatctcttc ctccgctagc atcaccacct cctcctcttc ctcctcttct 9480

ggcacttcca tgatggcttc ctcctcttcg gggggcggcg gcggcggcgg tgggggaggg 9540

ggcgctctgc gccggcggcg gcgcaccggg aggcggtcca cgaagcgcgc gatcatctcc 9600

ccgcggcggc ggcgcatggt ctcggtgacg gcgcggccgt tctcccgggg gcgcagttgg 9660

aagacgccgc cggacatctg gtgctggggc gggtggccgt gaggcagcga aacggcgctg 9720

acgatgcatc tcaacaattg ctgcgtaggt acgccgccga gggacctgag ggagtccata 9780

tccaccggat ccgaaaacct ttcgaggaag gcgtctaacc agtcgcagtc gcaaggtagg 9840

ctgagcaccg tggcgggcgg cggggggtgg ggggagtgtc tggcggaggt gctgctgatg 9900

atgtaattga agtaggcgga cttgacacgg cggatggtcg acaggagcac catgtccttg 9960

ggtccggcct gctggatgcg gaggcggtcg gctatgcccc aggcttcgtt ctggcatcgg 10020

cgcaggtcct tgtagtagtc ttgcatgagc ctttccaccg gcacctcttc tccttcctct 10080

tctgcttctt ccatgtctgc ttcggccctg gggcggcgcc gcgcccccct gccccccatg 10140

cgcgtgaccc cgaaccccct gagcggttgg agcagggcca ggtcggcgac gacgcgctcg 10200

gccaggatgg cctgctgcac ctgcgtgagg gtggtttgga agtcatccaa gtccacgaag 10260

cggtggtagg cgcccgtgtt gatggtgtag gtgcagttgg ccatgacgga ccagttgacg 10320

gtctggtggc ccggttgcga catctcggtg tacctgagtc gcgagtaggc gcgggagtcg 10380

aagacgtagt cgttgcaagt ccgcaccagg tactggtagc ccaccaggaa gtgcggcggc 10440

ggctggcggt agaggggcca gcgcagggtg gcgggggctc cgggggccag gtcttccagc 10500

atgaggcggt ggtaggcgta gatgtacctg gacatccagg tgatacccgc ggcggtggtg 10560

gaggcgcgcg ggaagtcgcg cacccggttc cagatgttgc gcaggggcag aaagtgctcc 10620

atggtaggcg tgctctgtcc agtcagacgc gcgcagtcgt tgatactcta gaccagggaa 10680

aacgaaagcc ggtcagcggg cactcttccg tggtctggtg aatagatcgc aagggtatca 10740

tggcggaggg cctcggttcg agccccgggt ccgggccgga cggtccgcca tgatccacgc 10800

ggttaccgcc cgcgtgtcga acccaggtgt gcgacgtcag acaacggtgg agtgttcctt 10860

ttggcgtttt tctggccggg cgccggcgtc gcgtaagaga ctaagccgcg aaagcgaaag 10920

cagtaagtgg ctcgctcccc gtagccggag ggatccttgc taagggttgc gttgcggcga 10980

accccggttc gaatcccgta ctcgggccgg ccggacccgc ggctaaggtg ttggattggc 11040

ctccccctcg tataaagacc ccgcttgcgg attgactccg gacacgggga cgagcccctt 11100

ttatttttgc tttccccaga tgcatccggt gctgcggcag atgcgccccc cgccccagca 11160

gcagcaacaa caccagcaag agcggcagca acagcagcgg gagtcatgca gggccccctc 11220

acccaccctc ggcgggccgg ccacctcggc gtccgcggcc gtgtctggcg cctgcggcgg 11280

cggcgggggg ccggctgacg accccgagga gcccccgcgg cgcagggcca gacactacct 11340

ggacctggag gagggcgagg gcctggcgcg gctgggggcg ccgtctcccg agcgccaccc 11400

gcgggtgcag ctgaagcgcg actcgcgcga ggcgtacgtg cctcggcaga acctgttcag 11460

ggaccgcgcg ggcgaggagc ccgaggagat gcgggacagg aggttcagcg cagggcggga 11520

gctgcggcag gggctgaacc gcgagcggct gctgcgcgag gaggactttg agcccgacgc 11580

gcggacgggg atcagccccg cgcgcgcgca cgtggcggcc gccgacctgg tgacggcgta 11640

cgagcagacg gtgaaccagg agatcaactt ccaaaagagt ttcaacaacc acgtgcgcac 11700

gctggtggcg cgcgaggagg tgaccatcgg gctgatgcac ctgtgggact ttgtaagcgc 11760

gctggtgcag aaccccaaca gcaagcctct gacggcgcag ctgttcctga tagtgcagca 11820

cagcagggac aacgaggcgt ttagggacgc gctgctgaac atcaccgagc ccgagggtcg 11880

gtggctgctg gacctgatta acatcctgca gagcatagtg gtgcaggagc gcagcctgag 11940

cctggccgac aaggtggcgg ccatcaacta ctcgatgctg agcctgggca agttttacgc 12000

gcgcaagatc taccagacgc cgtacgtgcc catagacaag gaggtgaaga tcgacggttt 12060

ttacatgcgc atggcgctga aggtgctcac cctgagcgac gacctgggcg tgtaccgcaa 12120

cgagcgcatc cacaaggccg tgagcgtgag ccggcggcgc gagctgagcg accgcgagct 12180

gatgcacagc ctgcagcggg cgctggcggg cgccggcagc ggcgacaggg aggcggagtc 12240

ctacttcgat gcgggggcgg acctgcgctg ggcgcccagc cggcgggccc tggaggccgc 12300

gggggtccgc gaggactatg acgaggacgg cgaggaggat gaggagtacg agctagagga 12360

gggcgagtac ctggactaaa ccgcgggtgg tgtttccggt agatgcaaga cccgaacgtg 12420

gtggacccgg cgctgcgggc ggctctgcag agccagccgt ccggccttaa ctcctcagac 12480

gactggcgac aggtcatgga ccgcatcatg tcgctgacgg cgcgtaaccc ggacgcgttc 12540

cggcagcagc cgcaggccaa caggctctcc gccatcctgg aggcggtggt gcctgcgcgc 12600

tcgaacccca cgcacgagaa ggtgctggcc atagtgaacg cgctggccga gaacagggcc 12660

atccgcccgg acgaggccgg gctggtgtac gacgcgctgc tgcagcgcgt ggcccgctac 12720

aacagcggca acgtgcagac caacctggac cggctggtgg gggacgtgcg cgaggcggtg 12780

gcgcagcgcg agcgcgcgga tcggcagggc aacctgggct ccatggtggc gctgaatgcc 12840

ttcctgagca cgcagccggc caacgtgccg cgggggcagg aagactacac caactttgtg 12900

agcgcgctgc ggctgatggt gaccgagacc ccccagagcg aggtgtacca gtcgggcccg 12960

gactacttct tccagaccag cagacagggc ctgcagacgg tgaacctgag ccaggctttc 13020

aagaacctgc gggggctgtg gggcgtgaag gcgcccaccg gcgaccgggc gacggtgtcc 13080

agcctgctga cgcccaactc gcgcctgctg ctgctgctga tcgcgccgtt cacggacagc 13140

ggcagcgtgt cccgggacac ctacctgggg cacctgctga ccctgtaccg cgaggccatc 13200

gggcaggcgc aggtggacga gcacaccttc caggagatca ccagcgtgag ccgcgcgctg 13260

gggcaggagg acacgagcag cctggaggcg actctgaact acctgctgac caaccggcgg 13320

cagaagattc cctcgctgca cagcctgacc tccgaggagg agcgcatctt gcgctacgtg 13380

cagcagagcg tgagcctgaa cctgatgcgc gacggggtga cgcccagcgt ggcgctggac 13440

atgaccgcgc gcaacatgga accgggcatg tacgccgcgc accggcctta catcaaccgc 13500

ctgatggact acctgcatcg cgcggcggcc gtgaaccccg agtactttac caacgccatc 13560

ctgaacccgc actggctccc gccgcccggg ttctacagcg ggggcttcga ggtcccggag 13620

gccaacgatg gcttcctgtg ggacgacatg gacgacagcg tgttctcccc gcggccgcag 13680

gcgctggcgg aagcgtccct gctgcgtccc aagaaggagg aggaggaggc gagtcgccgc 13740

cgcggcagca gcggcgtggc ttctctgtcc gagctggggg cggcagccgc cgcgcgcccc 13800

gggtccctgg gcggcagccc ctttccgagc ctggtggggt ctctgcacag cgagcgcacc 13860

acccgccctc ggctgctggg cgaggacgag tacctgaata actccctgct gcagccggtg 13920

cgggagaaaa acctgccccc cgccttcccc aacaacggga tagagagcct ggtggacaag 13980

atgagcagat ggaagaccta tgcgcaggag cacagggacg cgcccgcgct ccggccgccc 14040

acgcggcgcc agcgccacga ccggcagcgg gggctggtgt gggatgacga ggactccgcg 14100

gacgatagca gcgtgctgga cctgggaggg agcggcaacc cgttcgcgca cctgcgcccc 14160

cgcctgggga ggatgtttta aaaaaaaaaa aagcaagaag catgatgcaa aattaaataa 14220

aactcaccaa ggccatggcg accgagcgtt ggtttcttgt gttcccttca gtatgcggcg 14280

cgcggcgatg taccaggagg gacctcctcc ctcttacgag agcgtggtgg gcgcggcggc 14340

ggcggcgccc tcttctccct ttgcgtcgca gctgctggag ccgccgtacg tgcctccgcg 14400

ctacctgcgg cctacggggg ggagaaacag catccgttac tcggagctgg cgcccctgtt 14460

cgacaccacc cgggtgtacc tggtggacaa caagtcggcg gacgtggcct ccctgaacta 14520

ccagaacgac cacagcaatt ttttgaccac ggtcatccag aacaatgact acagcccgag 14580

cgaggccagc acccagacca tcaatctgga tgaccggtcg cactggggcg gcgacctgaa 14640

aaccatcctg cacaccaaca tgcccaacgt gaacgagttc atgttcacca ataagttcaa 14700

ggcgcgggtg atggtgtcgc gctcgcacac caaggaagac cgggtggagc tgaagtacga 14760

gtgggtggag ttcgagctgc cagagggcaa ctactccgag accatgacca ttgacctgat 14820

gaacaacgcg atcgtggagc actatctgaa agtgggcagg caaaacgggg tcctggagag 14880

cgacatcggg gtcaagttcg acaccaggaa cttccgcctg gggctggacc ccgtgaccgg 14940

gctggttatg cccggggtgt acaccaacga ggccttccat cccgacatca tcctgctgcc 15000

cggctgcggg gtggacttca cttacagccg cctgagcaac ctcctgggca tccgcaagcg 15060

gcagcccttc caggagggct tcaggatcac ctacgaggac ctggaggggg gcaacatccc 15120

cgcgctcctc gatgtggagg cctaccagga tagcttgaag gaaaatgagg cgggacagga 15180

ggataccacc cccgccgcct ccgccgccgc cgagcagggc gaggatgctg ctgacaccgc 15240

ggccgcggac ggggcagagg ccgaccccgc tatggtggtg gaggctcccg agcaggagga 15300

ggatatgaat gacagtgcgg tgcgcggaga caccttcgtc acccgggggg aggaaaagca 15360

agcggaggcc gaggccgcgg ccgaggaaaa gcaactggcg gcagcagcgg cggcggcggc 15420

gttggccgcg gcggaggctg agtctgaggg gaccaagccc gccaaggagc ccgtgattaa 15480

gcccctgacc gaagatagca agaagcgcag ttacaacctg ctcaaggaca gcaccaacac 15540

cgcgtaccgc agctggtacc tggcctacaa ctacggcgac ccgtcgacgg gggtgcgctc 15600

ctggaccctg ctgtgcacgc cggacgtgac ctgcggctcg gagcaggtgt actggtcgct 15660

gcccgacatg atgcaagacc ccgtgacctt ccgctccacg cggcaggtca gcaacttccc 15720

ggtggtgggc gccgagctgc tgcccgtgca ctccaagagc ttctacaacg accaggccgt 15780

ctactcccag ctcatccgcc agttcacctc tctgacccac gtgttcaatc gctttcctga 15840

gaaccagatt ctggcgcgcc cgcccgcccc caccatcacc accgtcagtg aaaacgttcc 15900

tgctctcaca gatcacggga cgctaccgct gcgcaacagc atcggaggag tccagcgagt 15960

gaccgttact gacgccagac gccgcacctg cccctacgtt tacaaggcct tgggcatagt 16020

ctcgccgcgc gtcctttcca gccgcacttt ttgagcaaca ccaccatcat gtccatcctg 16080

atctcaccca gcaataactc cggctgggga ctgctgcgcg cgcccagcaa gatgttcgga 16140

ggggcgagga agcgttccga gcagcacccc gtgcgcgtgc gcgggcactt ccgcgccccc 16200

tggggagcgc acaaacgcgg ccgcgcgggg cgcaccaccg tggacgacgc catcgactcg 16260

gtggtggagc aggcgcgcaa ctacaggccc gcggtctcta ccgtggacgc ggccatccag 16320

accgtggtgc ggggcgcgcg gcggtacgcc aagctgaaga gccgccggaa gcgcgtggcc 16380

cgccgccacc gccgccgacc cggggccgcc gccaaacgcg ccgccgcggc cctgcttcgc 16440

cgggccaagc gcacgggccg ccgcgccgcc atgagggccg cgcgccgctt ggccgccggc 16500

atcaccgccg ccaccatggc cccccgtacc cgaagacgcg cggccgccgc cgccgccgcc 16560

gccatcagtg acatggccag caggcgccgg ggcaacgtgt actgggtgcg cgactcggtg 16620

accggcacgc gcgtgcccgt gcgcttccgc cccccgcgga cttgagatga tgtgaaaaaa 16680

caacactgag tctcctgctg ttgtgtgtat cccagcggcg gcggcgcgcg cagcgtcatg 16740

tccaagcgca aaatcaaaga agagatgctc caggtcgtcg cgccggagat ctatgggccc 16800

ccgaagaagg aagagcagga ttcgaagccc cgcaagataa agcgggtcaa aaagaaaaag 16860

aaagatgatg acgatgccga tggggaggtg gagttcctgc gcgccacggc gcccaggcgc 16920

ccggtgcagt ggaagggccg gcgcgtaaag cgcgtcctgc gccccggcac cgcggtggtc 16980

ttcacgcccg gcgagcgctc cacccggact ttcaagcgcg tctatgacga ggtgtacggc 17040

gacgaagacc tgctggagca ggccaacgag cgcttcggag agtttgctta cgggaagcgt 17100

cagcgggcgc tggggaagga ggacctgctg gcgctgccgc tggaccaggg caaccccacc 17160

cccagtctga agcccgtgac cctgcagcag gtgctgccga gcagcgcacc ctccgaggcg 17220

aagcggggtc tgaagcgcga gggcggcgac ctggcgccca ccgtgcagct catggtgccc 17280

aagcggcaga ggctggagga tgtgctggag aaaatgaaag tagaccccgg tctgcagccg 17340

gacatcaggg tccgtcccat caagcaggtg gcgccgggcc tcggcgtgca gaccgtggac 17400

gtggtcatcc ccaccggcaa ctcccccgcc gccaccacca ctaccgctgc ctccacggac 17460

atggagacac agaccgatcc cgccgcagcc gcagccgccg ccgcagccgc gacctcctcg 17520

gcggaggtgc agacggaccc ctggctgccg ccggcgatgt cagctccccg cgcgcgccgc 17580

ggacgcagaa agtacggcgc cgccaacgcg ctcctgcccg agtacgcctt gcatccttcc 17640

atcgcgccca cccccggcta ccgaggctat acctaccgcc cgcgaagagc caagggttcc 17700

acccgccgtc cccgccgacg cgccgccgcc accacccgcc gccgccgccg cagacgccag 17760

cccgcactgg ctccagtctc cgtgaggaga gtggcgcgcg acggacacac cctggtgctg 17820

cccagggcgc gctaccaccc cagcatcgtt taaaagcctg ttgtggttct tgcagatatg 17880

gccctcactt gccgcctccg tttcccggtg ccgggatacc gaggaggaag atcgcgccgc 17940

aggaggggtc tggccggccg cggcctgagc ggaggcagcc gccgcgcgca ccggcggcga 18000

cgcgccacca gccgacgcat gcgcggcggg gtgctgcccc tgttaatccc cctgatcgcc 18060

gcggcgatcg gcgccgtgcc cgggatcgcc tccgtggcct tgcaagcgtc ccagaggcat 18120

tgacagactt gcaaacttgc aaatatggaa aaaaaaaaaa aaccccaata aaaagtctag 18180

actctcacgc tcgcttggtc ctgtgactat tttgtagaat ggaagacatc aactttgcgt 18240

cgctggcccc gcgtcacggc tcgcgcccgt tcctgggaca ctggaacgat atcggcacca 18300

gcaacatgag cggtggcgcc ttcagttggg gctctctgtg gagcggcatt aaaagtatcg 18360

ggtctgccgt taaaaattac ggctcccggg cctggaacag cagcacgggc cagatgttga 18420

gagacaagtt gaaagagcag aacttccagc agaaggtggt ggagggcctg gcctccggca 18480

tcaacggggt ggtggacctg gccaaccagg ccgtgcagaa taaaatcaac agcagactgg 18540

acccccggcc gccggtggag gaggtgccgc cggcgctgga gacggtgtcc cccgatgggc 18600

gtggcgagaa gcgcccgcgg cccgataggg aagagaccac tctggtcacg cagaccgatg 18660

agccgccccc gtatgaggag gccctaaagc aaggtctgcc caccacgcgg cccatcgcgc 18720

ccatggccac cggggtggtg ggccgccaca cccccgccac gctggacttg cctccgcccg 18780

ccgatgtgcc gcagcagcag aaggcggcac agccgggccc gcccgcgacc gcctcccgtt 18840

cctccgccgg tcctctgcgc cgcgcggcca gcggcccccg cgggggggtc gcgaggcacg 18900

gcaactggca gagcacgctg aacagcatcg tgggtctggg ggtgcggtcc gtgaagcgcc 18960

gccgatgcta ctgaatagct tagctaacgt gttgtatgtg tgtatgcgcc ctatgtcgcc 19020

gccagaggag ctgctgagtc gccgccgttc gcgcgcccac caccaccgcc actccgcccc 19080

tcaagatggc gaccccatcg atgatgccgc agtggtcgta catgcacatc tcgggccagg 19140

acgcctcgga gtacctgagc cccgggctgg tgcagttcgc ccgcgccacc gagagctact 19200

tcagcctgag taacaagttt aggaacccca cggtggcgcc cacgcacgat gtgaccaccg 19260

accggtctca gcgcctgacg ctgcggttca ttcccgtgga ccgcgaggac accgcgtact 19320

cgtacaaggc gcggttcacc ctggccgtgg gcgacaaccg cgtgctggac atggcctcca 19380

cctactttga catccgcggg gtgctggacc ggggtcccac tttcaagccc tactctggca 19440

ccgcctacaa ctccctggcc cccaagggcg ctcccaactc ctgcgagtgg gagcaagagg 19500

aaactcaggc agttgaagaa gcagcagaag aggaagaaga agatgctgac ggtcaagctg 19560

aggaagagca agcagctacc aaaaagactc atgtatatgc tcaggctccc ctttctggcg 19620

aaaaaattag taaagatggt ctgcaaatag gaacggacgc tacagctaca gaacaaaaac 19680

ctatttatgc agaccctaca ttccagcccg aaccccaaat cggggagtcc cagtggaatg 19740

aggcagatgc tacagtcgcc ggcggtagag tgctaaagaa atctactccc atgaaaccat 19800

gctatggttc ctatgcaaga cccacaaatg ctaatggagg tcagggtgta ctaacggcaa 19860

atgcccaggg acagctagaa tctcaggttg aaatgcaatt cttttcaact tctgaaaacg 19920

cccgtaacga ggctaacaac attcagccca aattggtgct gtatagtgag gatgtgcaca 19980

tggagacccc ggatacgcac ctttcttaca agcccgcaaa aagcgatgac aattcaaaaa 20040

tcatgctggg tcagcagtcc atgcccaaca gacctaatta catcggcttc agagacaact 20100

ttatcggcct catgtattac aatagcactg gcaacatggg agtgcttgca ggtcaggcct 20160

ctcagttgaa tgcagtggtg gacttgcaag acagaaacac agaactgtcc taccagctct 20220

tgcttgattc catgggtgac agaaccagat acttttccat gtggaatcag gcagtggaca 20280

gttatgaccc agatgttaga attattgaaa atcatggaac tgaagacgag ctccccaact 20340

attgtttccc tctgggtggc ataggggtaa ctgacactta ccaggctgtt aaaaccaaca 20400

atggcaataa cgggggccag gtgacttgga caaaagatga aacttttgca gatcgcaatg 20460

aaataggggt gggaaacaat ttcgctatgg agatcaacct cagtgccaac ctgtggagaa 20520

acttcctgta ctccaacgtg gcgctgtacc taccagacaa gcttaagtac aacccctcca 20580

atgtggacat ctctgacaac cccaacacct acgattacat gaacaagcga gtggtggccc 20640

cggggctggt ggactgctac atcaacctgg gcgcgcgctg gtcgctggac tacatggaca 20700

acgtcaaccc cttcaaccac caccgcaatg cgggcctgcg ctaccgctcc atgctcctgg 20760

gcaacgggcg ctacgtgccc ttccacatcc aggtgcccca gaagttcttt gccatcaaga 20820

acctcctcct cctgccgggc tcctacacct acgagtggaa cttcaggaag gatgtcaaca 20880

tggtcctcca gagctctctg ggtaacgatc tcagggtgga cggggccagc atcaagttcg 20940

agagcatctg cctctacgcc accttcttcc ccatggccca caacacggcc tccacgctcg 21000

aggccatgct caggaacgac accaacgacc agtccttcaa tgactacctt tccgccgcca 21060

acatgctcta ccccataccc gccaacgcca ccaacgtccc catctccatc ccctcgcgca 21120

actgggcggc cttccgcggc tgggccttca cccgcctcaa gaccaaggag accccctccc 21180

tgggctcggg attcgacccc tactacacct actcgggctc tattccctac ctggacggca 21240

ccttctacct caaccacact ttcaagaagg tctcggtcac cttcgactcc tcggtcagct 21300

ggccgggcaa cgaccgtctg ctcaccccca acgagttcga gatcaagcgc tcggtcgacg 21360

gggaaggcta caacgtggcc cagtgcaaca tgaccaagga ctggttcctg gtccagatgc 21420

tggccaacta caacatcggc taccagggct tctacatccc agagagctac aaggacagga 21480

tgtactcctt cttcaggaac ttccagccca tgagccggca ggtggtggac cagaccaagt 21540

acaaggacta ccaggaggtg ggcatcatcc accagcacaa caactcgggc ttcgtgggct 21600

acctcgcccc caccatgcgc gagggacagg cctaccccgc caacttcccc tacccgctca 21660

taggcaagac cgcggtcgac agcatcaccc agaaaaagtt cctctgcgac cgcaccctct 21720

ggcgcatccc cttctccagc aacttcatgt ccatgggtgc gctctcggac ctgggccaga 21780

acttgctcta cgccaactcc gcccacgccc tcgacatgac cttcgaggtc gaccccatgg 21840

acgagcccac ccttctctat gttctgttcg aagtctttga cgtggtccgg gtccaccagc 21900

cgcaccgcgg cgtcatcgag accgtgtacc tgcgtacgcc cttctcggcc ggcaacgcca 21960

ccacctaaag aagcaagccg cagtcatcgc cgcctgcatg ccgtcgggtt ccaccgagca 22020

agagctcagg gccatcgtca gagacctggg atgcgggccc tattttttgg gcaccttcga 22080

caagcgcttc cctggctttg tctccccaca caagctggcc tgcgccatcg tcaacacggc 22140

cggccgcgag accgggggcg tgcactggct ggcctttgcc tggaacccgc gctccaaaac 22200

atgcttcctc tttgacccct tcggcttttc ggaccagcgg ctcaagcaaa tctacgagtt 22260

cgagtacgag ggcttgctgc gtcgcagcgc catcgcctcc tcgcccgacc gctgcgtcac 22320

cctcgaaaag tccacccaga ccgtgcaggg gcccgactcg gccgcctgcg gtctcttctg 22380

ctgcatgttt ctgcacgcct ttgtgcactg gcctcagagt cccatggacc gcaaccccac 22440

catgaacttg ctgacggggg tgcccaactc catgctccaa agcccccagg tcgagcccac 22500

cctgcgccgc aaccaggagc agctctacag cttcctggag cgccactcgc cctacttccg 22560

ccgccacagc gcacagatca ggagggccac ctccttctgc cacttgcaag agatgcaaga 22620

agggtaataa cgatgtacac acttttttct caataaatgg catttttttt ttatttatac 22680

aagctctctg gggtattcat ttcccaccac caccacccgc cgttgtcgcc atctggctct 22740

atttagaaat cgaaagggtt ctgccgggag tcgccgtgcg ccacgggcag ggacacgttg 22800

cgatactggt agcgggtgcc ccacttgaac tcgggcacca ccaggcgagg cagctcgggg 22860

aagttttcgc tccacaggct gcgggtcagc accagcgcgt tcatcaggtc gggcgccgag 22920

atcttgaagt cgcagttggg gccgccgccc tgcgcgcgcg agttgcggta caccgggttg 22980

cagcactgga acaccaacag cgccgggtgc ttcacgctgg ccagcacgct gcggtcggag 23040

atcagctcgg cgtccaggtc ctccgcgttg ctcagcgcga acggggtcat cttgggcact 23100

tgccgcccca ggaagggcgc gtgccccggt ttcgagttgc agtcgcagcg cagcgggatc 23160

agcaggtgcc cgtgcccgga ctcggcgttg gggtacagcg cgcgcatgaa ggcctgcatc 23220

tggcggaagg ccatctgggc cttggcgccc tccgagaaga acatgccgca ggacttgccc 23280

gagaactggt ttgcggggca gctggcgtcg tgcaggcagc agcgcgcgtc ggtgttggcg 23340

atctgcacca cgttgcgccc ccaccggttc ttcacgatct tggccttgga cgattgctcc 23400

ttcagcgcgc gctgcccgtt ctcgctggtc acatccatct cgatcacatg ttccttgttc 23460

accatgctgc tgccgtgcag acacttcagc tcgccctccg tctcggtgca gcggtgctgc 23520

cacagcgcgc agcccgtggg ctcgaaagac ttgtaggtca cctccgcgaa ggactgcagg 23580

tacccctgca aaaagcggcc catcatggtc acgaaggtct tgttgctgct gaaggtcagc 23640

tgcagcccgc ggtgctcctc gttcagccag gtcttgcaca cggccgccag cgcctccacc 23700

tggtcgggca gcatcttgaa gttcaccttc agctcattct ccacgtggta cttgtccatc 23760

agcgtgcgcg ccgcctccat gcccttctcc caggccgaca ccagcggcag gctcacgggg 23820

ttcttcacca tcaccgtggc cgccgcctcc gccgcgcttt cgctttccgc cccgctgttc 23880

tcttcctctt cctcctcttc ctcgccgccg cccactcgca gcccccgcac cacggggtcg 23940

tcttcctgca ggcgctgcac cttgcgcttg ccgttgcgcc cctgcttgat gcgcacgggc 24000

gggttgctga agcccaccat caccagcgcg gcctcttctt gctcgtcctc gctgtccaga 24060

atgacctccg gggagggggg gttggtcatc ctcagtaccg aggcacgctt ctttttcttc 24120

ctgggggcgt tcgccagctc cgcggctgcg gccgctgccg aggtcgaagg ccgagggctg 24180

ggcgtgcgcg gcaccagcgc gtcttgcgag ccgtcctcgt cctcctcgga ctcgagacgg 24240

aggcgggccc gcttcttcgg gggcgcgcgg ggcggcggag gcggcggcgg cgacggagac 24300

ggggacgaga catcgtccag ggtgggtgga cggcgggccg cgccgcgtcc gcgctcgggg 24360

gtggtttcgc gctggtcctc ttcccgactg gccatctccc actgctcctt ctcctatagg 24420

cagaaagaga tcatggagtc tctcatgcga gtcgagaagg aggaggacag cctaaccgcc 24480

ccctctgagc cctccaccac cgccgccacc accgccaatg ccgccgcgga cgacgcgccc 24540

accgagacca ccgccagtac caccctcccc agcgacgcac ccccgctcga gaatgaagtg 24600

ctgatcgagc aggacccggg ttttgtgagc ggagaggagg atgaggtgga tgagaaggag 24660

aaggaggagg tcgccgcctc agtgccaaaa gaggataaaa agcaagacca ggacgacgca 24720

gataaggatg agacagcagt cgggcggggg aacggaagcc atgatgctga tgacggctac 24780

ctagacgtgg gagacgacgt gctgcttaag cacctgcacc gccagtgcgt catcgtctgc 24840

gacgcgctgc aggagcgctg cgaagtgccc ctggacgtgg cggaggtcag ccgcgcctac 24900

gagcggcacc tcttcgcgcc gcacgtgccc cccaagcgcc gggagaacgg cacctgcgag 24960

cccaacccgc gtctcaactt ctacccggtc ttcgcggtac ccgaggtgct ggccacctac 25020

cacatcttct tccaaaactg caagatcccc ctctcctgcc gcgctaaccg cacccgcgcc 25080

gacaaaaccc tgaccctgcg gcagggcgcc cacatacctg atattgcctc tctggaggaa 25140

gtgcccaaga tcttcgaggg tctcggtcgc gacgagaaac gggcggcgaa cgctctgcac 25200

ggagacagcg aaaacgagag tcactcgggg gtgctggtgg agctcgaggg cgacaacgcg 25260

cgcctggccg tactcaagcg cagcatagag gtcacccact ttgcctaccc ggcgctcaac 25320

ctgcccccca aggtcatgag tgtggtcatg ggcgagctca tcatgcgccg cgctcagccc 25380

ctggccgcgg atgcaaactt gcaagagtcc tccgaggaag gcctgcccgc ggtcagcgac 25440

gagcagctag cgcgctggct ggagacccgc gaccccgcgc agctggagga gcggcgcaag 25500

ctcatgatgg ccgcggtgct ggtcaccgtg gagctcgagt gtctgcagcg cttcttcgcg 25560

gaccccgaga tgcagcgcaa gctcgaggag accctgcact acaccttccg ccagggctac 25620

gtgcgccagg cctgcaagat ctccaacgtg gagctctgca acctggtctc ctacctgggc 25680

atcctgcacg agaaccgcct cgggcagaac gtcctgcact ccaccctcaa aggggaggcg 25740

cgccgcgact acatccgcga ctgcgcctac ctcttcctct gctacacctg gcagacggcc 25800

atgggggtct ggcagcagtg cctggaggag cgcaacctca aggagctgga aaagctactc 25860

aagcgcaccc tcagggacct ctggacgggc ttcaacgagc gctcggtggc cgccgcgctg 25920

gcggacatca tcttccccga gcgcctgctc aagaccctgc agcagggcct gcccgacttc 25980

accagccaga gcatgctgca gaactttagg actttcatcc tggagcgctc gggcatcctg 26040

cctgccactt gctgcgcgct gcccagcgac ttcgtgccca tcaagtacag ggagtgcccg 26100

ccgccgctct ggggccactg ctacctcttc cagctggcca actacctcgc ctaccactcg 26160

gacctcatgg aagacgtgag cggcgagggc ctgctcgagt gccactgccg ctgcaacctc 26220

tgcacgcccc accgctctct agtctgcaac ccgcagctgc tcagcgagag tcagattatc 26280

ggtaccttcg agctgcaggg tccctcgcct gacgagaagt ccgcggctcc ggggctgaaa 26340

ctcactccgg ggctgtggac ttccgcctac ctacgcaaat ttgtacctga ggactaccac 26400

gcccacgaga tcaggttcta cgaagaccaa tcccgcccgc ccaaggcgga gctcaccgcc 26460

tgcgtcatca cccaggggca catcctgggc caattgcaag ccatcaacaa agcccgccga 26520

gagttcttgc tgaaaaaggg tcggggggtg tacctggacc cccagtccgg cgaggagcta 26580

aacccgctac ccccgccgcc gccccagcag cgggaccttg cttcccagga tggcacccag 26640

aaagaagcag cagccgccgc cgccgcagcc atacatgctt ctggaggaag aggaggagga 26700

ctgggacagt caggcagagg aggtttcgga cgaggagcag gaggagatga tggaagactg 26760

ggaggaggac agcagcctag acgaggaagc ttcagaggcc gaagaggtgg cagacgcaac 26820

accatcaccc tcggtcgcag ccccctcgcc ggggcccctg aaatcctccg aacccagcac 26880

cagcgctata acctccgctc ctccggcgcc ggcgccaccc gcccgcagac ccaaccgtag 26940

atgggacacc acaggaaccg gggtcggtaa gtccaagtgc ccgccgccgc caccgcagca 27000

gcagcagcag cgccagggct accgctcgtg gcgcgggcac aagaacgcca tagtcgcctg 27060

cttgcaagac tgcgggggca acatctcttt cgcccggcgc ttcctgctat tccaccacgg 27120

ggtcgccttt ccccgcaatg tcctgcatta ctaccgtcat ctctacagcc cctactgcag 27180

cggcgaccca gaggcggcag cggcagccac agcggcgacc accacctagg aagatatcct 27240

ccgcgggcaa gacagcggca gcagcggcca ggagacccgc ggcagcagcg gcgggagcgg 27300

tgggcgcact gcgcctctcg cccaacgaac ccctctcgac ccgggagctc agacacagga 27360

tcttccccac tttgtatgcc atcttccaac agagcagagg ccaggagcag gagctgaaaa 27420

taaaaaacag atctctgcgc tccctcaccc gcagctgtct gtatcacaaa agcgaagatc 27480

agcttcggcg cacgctggag gacgcggagg cactcttcag caaatactgc gcgctcactc 27540

ttaaagacta gctccgcgcc cttctcgaat ttaggcggga gaaaactacg tcatcgccgg 27600

ccgccgccca gcccgcccag ccgagatgag caaagagatt cccacgccat acatgtggag 27660

ctaccagccg cagatgggac tcgcggcggg agcggcccag gactactcca cccgcatgaa 27720

ctacatgagc gcgggacccc acatgatctc acaggtcaac gggatccgcg cccagcgaaa 27780

ccaaatactg ctggaacagg cggccatcac cgccacgccc cgccataatc tcaacccccg 27840

aaattggccc gccgccctcg tgtaccagga aaccccctcc gccaccaccg tactacttcc 27900

gcgtgacgcc caggccgaag tccagatgac taactcaggg gcgcagctcg cgggcggctt 27960

tcgtcacggg gcgcggccgc tccgaccagg tataagacac ctgatgatca gaggccgagg 28020

tatccagctc aacgacgagt cggtgagctc ttcgctcggt ctccgtccgg acggaacttt 28080

ccagctcgcc ggatccggcc gctcttcgtt cacgccccgc caggcgtacc tgactctgca 28140

gacctcgtcc tcggagcccc gctccggagg catcggaacc ctccagttcg tggaggagtt 28200

cgtgccctcg gtctacttca accccttctc gggacctccc ggacgctacc ccgaccagtt 28260

cattccgaac tttgacgcgg tgaaggactc ggcggacggc tacgactgaa tgtcaggtgc 28320

cgaggcagag cagcttcgcc tgagacacct cgagcactgc cgccgccaca agtgcttcgc 28380

ccgcggttcc ggtgagttct gctactttca gctacccgag gagcataccg aggggccggc 28440

gcacggcgtc cgcctgacca cccagggcga ggttacctgt tccctcatcc gggagttcac 28500

cctccgtccc ctgctagtgg agcgggagcg gggtccctgt gtcctaacta tcgcctgcaa 28560

ctgccctaac cctggattac atcaagatct ttgctgtcat ctctgtgctg agtttaataa 28620

acgctgagat cagaatctac tggggctcct gtcgccatcc tgtgaacgcc accgtcttca 28680

cccaccccga ccaggcccag gcgaacctca cctgcggtct gcatcggagg gccaagaagt 28740

acctcacctg gtacttcaac ggcaccccct ttgtggttta caacagcttc gacggggacg 28800

gagtctccct gaaagaccag ctctccggtc tcagctactc catccacaag aacaccaccc 28860

tccaactctt ccctccctac ctgccgggaa cctacgagtg cgtcaccggc cgctgcaccc 28920

acctcacccg cctgatcgta aaccagagct ttccgggaac agataactcc ctcttcccca 28980

gaacaggagg tgagctcagg aaactccccg gggaccaggg cggagacgta ccttcgaccc 29040

ttgtggggtt aggatttttt attaccgggt tgctggctct tttaatcaaa gcttccttga 29100

gatttgttct ttccttctac gtgtatgaac acctcagcct ccaataactc taccctttct 29160

tcggaatcag gtgacttctc tgaaatcggg cttggtgtgc tgcttactct gttgattttt 29220

ttccttatca tactcagcct tctgtgcctc aggctcgccg cctgctgcgc acacatctat 29280

atctactgct ggttgctcaa gtgcaggggt cgccacccaa gatgaacagg tacatggtcc 29340

tatcgatcct aggcctgctg gccctggcgg cctgcagcgc cgccaaaaaa gagattacct 29400

ttgaggagcc cgcttgcaat gtaactttca agcccgaggg tgaccaatgc accaccctcg 29460

tcaaatgcgt taccaatcat gagaggctgc gcatcgacta caaaaacaaa actggccagt 29520

ttgcggtcta tagtgtgttt acgcccggag acccctctaa ctactctgtc accgtcttcc 29580

agggcggaca gtctaagata ttcaattaca ctttcccttt ttatgagtta tgcgatgcgg 29640

tcatgtacat gtcaaaacag tacaacctgt ggcctccctc tccccaggcg tgtgtggaaa 29700

atactgggtc ttactgctgt atggctttgg caatcactac gctcgctcta atctgcacgg 29760

tgctatacat aaaattcagg cagaggcgaa tctttatcga tgaaaagaaa atgccttgat 29820

cgctaacacc ggctttctat ctgcagaatg aatgcaatca cctccctact aatcaccacc 29880

accctccttg cgattgccca tgggttgaca cgaatcgaag tgccagtggg gtccaatgtc 29940

accatggtgg gccccgccgg caattccacc ctcatgtggg aaaaatttgt ccgcaatcaa 30000

tgggttcatt tctgctctaa ccgaatcagt atcaagccca gagccatctg cgatgggcaa 30060

aatctaactc tgatcaatgt gcaaatgatg gatgctgggt actattacgg gcagcgggga 30120

gaaatcatta attactggcg accccacaag gactacatgc tgcatgtagt cgaggcactt 30180

cccactacca cccccactac cacctctccc accaccacta ccaccactac tactactact 30240

actaccacta ccgctgcccg ccatacccgc aaaagcacca tgattagcac aaagccccct 30300

cgtgctcact cccacgccgg cgggcccatc ggtgcgacct cagaaaccac cgagctttgc 30360

ttctgccaat gcactaacgc cagcgctcat gaactgttcg acctggagaa tgaggatgcc 30420

cagcagagct ccgcttgcct gacccaggag gctgtggagc ccgttgccct gaagcagatc 30480

ggtgattcaa taattgactc ttcttctttt gccactcccg aataccctcc cgattctact 30540

ttccacatca cgggtaccaa agaccctaac ctctctttct acctgatgct gctgctctgt 30600

atctctgtgg tctcttccgc gctgatgtta ctggggatgt tctgctgcct gatctgccgc 30660

agaaagagaa aagctcgctc tcagggccaa ccactgatgc ccttccccta ccccccggat 30720

tttgcagata acaagatatg agctcgctgc tgacactaac cgctttacta gcctgcgctc 30780

taacccttgt cgcttgcgac tcgagattcc acaatgtcac agctgtggca ggagaaaatg 30840

ttactttcaa ctccacggcc gatacccagt ggtcgtggag tggctcaggt agctacttaa 30900

ctatctgcaa tagctccact tcccccagca tatccccaac caagtaccaa tgcaatgcca 30960

gcctgttcac cctcatcaac gcttccaccc tggacaatgg actctatgta ggctatgtac 31020

cctttggtgg gcaaggaaag acccacgctt acaacctgga agttcgccag cccagaacca 31080

ctacccaagc ttctcccacc accaccacca ccaccaccac caccatcacc agcagcagca 31140

gcagccacag cagcagcagc agattattga ctttggtttt ggccagctca tctgccgcta 31200

cccaggccat ctacagctct gtgcccgaaa ccactcagat ccaccgccca gaaacgacca 31260

ccgccaccac cctacacacc tccagcgatc agatgccgac caacatcacc cccttggctc 31320

ttcaaatggg acttacaagc cccactccaa aaccagtgga tgcggccgag gtctccgccc 31380

tcgtcaatga ctgggcgggg ctgggaatgt ggtggttcgc cataggcatg atggcgctct 31440

gcctgcttct gctctggctc atctgctgcc tccaccgcag gcgagccaga ccccccatct 31500

atagacccat cattgtcctg aaccccgata atgatgggat ccatagattg gatggcctga 31560

aaaacctact tttttctttt acagtatgat aaattgagac atgcctcgca ttttcttgta 31620

catgttcctt ctcccacctt ttctggggtg ttctacgctg gccgctgtgt ctcacctgga 31680

ggtagactgc ctctcaccct tcactgtcta cctgctttac ggattggtca ccctcactct 31740

catctgcagc ctaatcacag taatcatcgc cttcatccag tgcattgatt acatctgtgt 31800

gcgcctcgca tacttcagac accacccgca gtaccgagac aggaacattg cccaacttct 31860

aagactgctc taatcatgca taagactgtg atctgccttc tgatcctctg catcctgccc 31920

accctcacct cctgccagta caccacaaaa tctccgcgca aaagacatgc ctcctgccgc 31980

ttcacccaac tgtggaatat acccaaatgc tacaacgaaa agagcgagct ctccgaagct 32040

tggctgtatg gggtcatctg tgtcttagtt ttctgcagca ctgtctttgc cctcatgatc 32100

tacccctact ttgatttggg atggaacgcg atcgatgcca tgaattaccc cacctttccc 32160

gcacccgaga taattccact gcgacaagtt gtacccgttg tcgttaatca acgcccccca 32220

tcccctacgc ccactgaaat cagctacttt aacctaacag gcggagatga ctgacgccct 32280

agatctagaa atggacggca tcagtaccga gcagcgtctc ctagagaggc gcaggcaggc 32340

ggctgagcaa gagcgcctca atcaggagct ccgagatctc gttaacctgc accagtgcaa 32400

aagaggcatc ttttgtctgg taaagcaggc caaagtcacc tacgagaaga ccggcaacag 32460

ccaccgcctc agttacaaat tgcccaccca gcgccagaag ctggtgctca tggtgggtga 32520

gaatcccatc accgtcaccc agcactcggt agagaccgag gggtgtctgc actctccctg 32580

tcggggtcca gaagacctct gcaccctggt aaagaccctg tgcggtctca gagatttagt 32640

cccctttaac taatcaaaca ctggaatcaa taaaaagaat cacttactta aaatcagaca 32700

gcaggtctct gtccagttta ttcagcagca cctccttccc ctcctcccaa ctctggtact 32760

ccaaacgcct tctggcggca aacttcctcc acaccctgaa gggaatgtca gattcttgct 32820

cctgtccctc cgcacccact atcttcatgt tgttgcagat gaagcgcacc aaaacgtctg 32880

acgagagctt caaccccgtg tacccctatg acacggaaag cggccctccc tccgtccctt 32940

tcctcacccc tcccttcgtg tctcccgatg gattccaaga aagccccccc ggggtcctgt 33000

ctctgaacct ggccgagccc ctggtcactt cccacggcat gctcgccctg aaaatgggaa 33060

gtggcctctc cctggacgac gctggcaacc tcacctctca agatatcacc accgctagcc 33120

ctcccctcaa aaaaaccaag accaacctca gcctagaaac ctcatccccc ctaactgtaa 33180

gcacctcagg cgccctcacc gtagcagccg ccgctcccct ggcagtggcc ggcacctccc 33240

tcaccatgca atcagaggcc cccctgacag tacaggatgc aaaactcacc ctggccacca 33300

aaggccccct gaccgtgtct gaaggcaaac tggccttgca aacatcggcc ccgctgacgg 33360

ccgctgacag cagcaccctc accgttagcg ccacaccacc aattaatgta agcagtggaa 33420

gtttaggctt agacatggaa gaccctatgt atactcacga tggaaaactg ggaataagaa 33480

ttgggggtcc actaagagta gtagacagct tgcacacact cactgtagtt accggaaatg 33540

gactaactgt agataacaat gccctccaaa ctagagttac gggcgcccta ggttatgaca 33600

catcaggaaa tctacaattg agagctgcag gaggtatgcg aattgatgca aatggccaac 33660

ttatccttaa tgtggcatac ccatttgatg ctcagaacaa tctcagcctt agacttggtc 33720

agggacccct gtatataaac acagaccaca acctggattt gaattgcaac agaggtctaa 33780

ccacaactac caccaacaac acaaaaaaac ttgagactaa aattagctca ggcttagact 33840

atgacaccaa tggtgctgtc attattaaac ttggcactgg tctaagcttc gacaacacag 33900

gcgccctaac tgtgggaaac actggtgatg ataaactgac tctgtggacg accccagacc 33960

catctccaaa ttgcagaatt cactcagaca aagactgcaa gtttactcta gtcctaacta 34020

agtgtggaag ccaaatcctg gcctctgtcg ccgccctagc ggtatcagga aatctggctt 34080

cgataacagg caccgttgcc agcgttacca tctttctcag atttgatcag aatggagtgc 34140

ttatggaaaa ctcctcgcta gacaggcagt actggaactt cagaaatggc aactcaacta 34200

acgctgcccc ctacaccaat gcagttgggt tcatgccaaa cctcgcagca taccccaaaa 34260

cgcaaagcca gactgctaaa aacaacattg taagtcaggt ttacttgaat ggagacaaat 34320

ccaaacccat gacccttacc atcaccctca atggaactaa tgaatccagt gaaactagcc 34380

aggtgagtca ctactccatg tcatttacat gggcttggga aagtgggcaa tatgccactg 34440

aaacctttgc caccaactcc ttcacctttt cttacattgc tgaacaataa aaagcatgac 34500

actgatgttc atttctgatt cttattttat tattttcaaa cacaacaaaa tcattcaagt 34560

cattcttcca tcttagctta atagacacag tagcttaata gacccagtag tgcaaagccc 34620

cattctagct tatagatcag acagtgataa ttaaccacca ccaccaccat accttttgat 34680

tcaggaaatc atgatcatca caggatccta gtcttcaggc cgccccctcc ctcccaagac 34740

acagaataca cagtcctctc cccccgactg gctttaaata acaccatctg gttggtcaca 34800

gacatgttct taggggttat attccacacg gtctcctgcc gcgccaggcg ctcgtcggtg 34860

atgttgataa actctcccgg cagctcgctc aagttcacgt cgctgtccag cggctgaacc 34920

tccggctgac gcgataactg tgcgaccggc tgctggacaa acggaggccg cgcctacaag 34980

ggggtagagt cataatcctc ggtcaggata gggcggtgat gcagcagcag cgagcgaaac 35040

atctgctgcc gccgccgctc cgtccggcag gaaaacaaca agccggtggt ctcctccgcg 35100

ataatccgca ccgcccgcag catcagcttc ctcgttctcc gcgcgcagca cctcaccctg 35160

atctcgctca agtcggcgca gtaggtacag cacagcacca cgatgttatt catgatccca 35220

cagtgcaggg cgctgtatcc aaagctcatg ccgggaacca ccgcccccac gtggccatcg 35280

taccacaagc gcacgtaaat taagtgtcga cccctcatga acgtgctgga cacaaacatt 35340

acttccttgg gcatgttgta attcaccacc tcccggtacc agataaacct ctggttaaac 35400

agggcacctt ccaccaccat cctgaaccaa gaggccagaa cctgcccacc ggctatgcac 35460

tgcagggaac ccgggttgga acaatgacaa tgcagactcc aaggctcgta accgtggatc 35520

atccggctgc tgaaggcatc gatgttggca caacacagac acacgtgcat gcactttctc 35580

atgattagca gctcttccct cgtcaggatc atatcccaag gaataaccca ttcttgaatc 35640

aacgtaaaac ccacacagca gggaaggcct cgcacataac tcacgttgtg catggtcagc 35700

gtgttgcatt ctggaaacag cggatgatcc tccagtatcg aggcgcgggt ctccttctca 35760

cagggaggta aagggtccct gctgtacgga ctgcgccggg acgaccgaga tcgtgttgag 35820

cgtagtgtca tggaaaaggg aacgccggac gtggtcatac ttcttgaagc agaaccaggt 35880

tcgcgcgtgg caggcctcct tgcgtctgcg gtctcgccgt ctagctcgct ccgtgtgata 35940

gttgtagtac agccactccc gcagagcgtc gaggcgcacc ctggcttccg gatctatgta 36000

gactccgtct tgcaccgcgg ccctgataat atccaccacc gtagaataag caacacccag 36060

ccaagcaata cactcgctct gcgagcggca gacaggagga gcgggcagag atgggagaac 36120

catgataaaa aacttttttt aaagaatatt ttccaattct tcgaaagtaa gatctatcaa 36180

gtggcagcgc tcccctccac tggcgcggtc aaactctacg gccaaagcac agacaacggc 36240

atttctaaga tgttccttaa tggcgtccaa aagacacacc gctctcaagt tgcagtaaac 36300

tatgaatgaa aacccatccg gctgattttc caatatagac gcgccggcgg cgtccaccaa 36360

acccagataa ttttcttctc tccagcggtt tagaatctgt ctaagcaaat cccttatatc 36420

aagtccggcc atgccaaaaa tctgctcaag agcgccctcc accttcatga ccaagcagcg 36480

catcatgatt gcaaaaattc aggttcttca gagacctgta taagattcaa aatgggaaca 36540

ttaacaaaaa ttcctctgtc gcgcagatcc cttcgcaggg caagctgaac ataatcagac 36600

aggtctgaac ggaccagtga ggccaaatcc ccaccaggaa ccagatccag agaccctata 36660

ctgattatga cgcgcatact cggggctatg ctgaccagcg tagcgccgat gtaggcgtgc 36720

tgcatgggcg gcgagataaa atgcaaagtg ctggttaaaa aatcaggcaa agcctcgcgc 36780

aaaaaagcta acacatcata atcatgctca tgcaggtagt tgcaggtaag ctcaggaacc 36840

aaaacggaat aacacacgat tttcctctca aacatgactt cgcggatact gcgtaaaaca 36900

aaaattataa ataaaaaatt aattaactta aacattggaa gcctgtctca caacaggaaa 36960

aaccacttta atcaacataa gacgggccac gggcatgccg gcatagccgt aaaaaaattg 37020

gtccccgtga ttaacaagta ccacagacag ctccccggtc atgtcggggg tcatcatgtg 37080

agactctgta tacacgtctg gattgtgaac atcagacaaa caaagaaatc gagccacgta 37140

gcccggaggt ataatcaccc gcaggcggag gtacagcaaa acgaccccca taggaggaat 37200

cacaaaatta gtaggagaaa aaaatacata aacaccagaa aaaccctgtt gctgaggcaa 37260

aatagcgccc tcccgatcca aaacaacata aagcgcttcc acaggagcag ccataacaaa 37320

gacccgagtc ttaccagtaa aagaaaaaag atctctcaac gcagcaccag caccaacact 37380

tcgcagtgta aaaggccaag tgccgagaga gtatatatag gaataaaaag tgacgtaaac 37440

gggcaaagtc caaaaaacgc ccagaaaaac cgcacgcgaa cctacgcccc gaaacgaaag 37500

ccaaaaaaca ctagacactc ccttccggcg tcaacttccg ctttcccacg ctacgtcact 37560

tgccccagtc aaacaaacta catatcccga acttccaagt cgccacgccc aaaacaccgc 37620

ctacacctcc ccgcccgccg gcccgccccc aaacccgcct cccgccccgc gccccgcctc 37680

gcgccgccca tctcattatc atattggctt caatccaaaa taaggtatat tattgatgat 37740

g 37741

<210> 2

<211> 36519

<212> DNA

<213> Pan troglodytes

<400> 2

ccatcttcaa taatatacct caaacttttt gtgcgcgtta atatgcaaat gaggcgtttg 60

aatttgggga ggaagggcgg tgattggtcg agggatgagc gaccgttagg ggcggggcga 120

gtgacgtttt gatgacgtgg ttgcgaggag gagccagttt gcaagttctc gtgggaaaag 180

tgacgtcaaa cgaggtgtgg tttgaacacg gaaatactca attttcccgc gctctctgac 240

aggaaatgag gtgtttctgg gcggatgcaa gtgaaaacgg gccattttcg cgcgaaaact 300

gaatgaggaa gtgaaaatct gagtaatttc gcgtttatgg cagggaggag tatttgccga 360

gggccgagta gactttgacc gattacgtgg gggtttcgat taccgtgttt ttcacctaaa 420

tttccgcgta cggtgtcaaa gtccggtgtt tttacgtagg tgtcagctga tcgccagggt 480

atttaaacct gcgctctcca gtcaagaggc cactcttgag tgccagcgag aagagttttc 540

tcctccgcgc cgcgagtcag atctacactt tgaaagatga ggcacctgag agacctgccc 600

gatgagaaaa tcatcatcgc ttccgggaac gagattctgg aactggtggt aaatgccatg 660

atgggcgacg accctccgga gccccccacc ccatttgaga caccttcgct gcacgatttg 720

tatgatctgg aggtggatgt gcccgaggac gatcccaatg aggaggcggt aaatgatttt 780

tttagcgatg ccgcgctgct agctgccgag gaggcttcga gctctagctc agacagcgac 840

tcttcactgc atacccctag acccggcaga ggtgagaaaa agatccccga gcttaaaggg 900

gaagagatgg acttgcgctg ctatgaggaa tgcttgcccc cgagcgatga tgaggacgag 960

caggcgatcc agaacgcagc gagccaggga gtgcaagccg ccagcgagag ctttgcgctg 1020

gactgcccgc ctctgcccgg acacggctgt aagtcttgtg aatttcatcg catgaatact 1080

ggagataaag ctgtgttgtg tgcactttgc tatatgagag cttacaacca ttgtgtttac 1140

agtaagtgtg attaagttga actttagagg gaggcagaga gcagggtgac tgggcgatga 1200

ctggtttatt tatgtatata tgttctttat ataggtcccg tctctgacgc agatgatgag 1260

acccccacta caaagtccac ttcgtcaccc ccagaaattg gcacatctcc acctgagaat 1320

attgttagac cagttcctgt tagagccact gggaggagag cagctgtgga atgtttggat 1380

gacttgctac agggtggggt tgaacctttg gacttgtgta cccggaaacg ccccaggcac 1440

taagtgccac acatgtgtgt ttacttgagg tgatgtcagt atttataggg tgtggagtgc 1500

aataaaaaat gtgttgactt taagtgcgtg gtttatgact caggggtggg gactgtgagt 1560

atataagcag gtgcagacct gtgtggttag ctcagagcgg catggagatt tggacggtct 1620

tggaagactt tcacaagact agacagctgc tagagaacgc ctcgaacgga gtctcttacc 1680

tgtggagatt ctgcttcggt ggcgacctag ctaggctagt ctacagggcc aaacaggatt 1740

atagtgaaca atttgaggtt attttgagag agtgttctgg tctttttgac gctcttaact 1800

tgggccatca gtctcacttt aaccagagga tttcgagagc ccttgatttt actactcctg 1860

gcagaaccac tgcagcagta gccttttttg cttttattct tgacaaatgg agtcaagaaa 1920

cccatttcag cagggattac cagctggatt tcttagcagt agctttgtgg agaacatgga 1980

agtgccagcg cctgaatgca atctccggct acttgccggt acagccgcta gacactctga 2040

ggatcctgaa tctccaggag agtcccaggg cacgccaacg tcgccagcag cagcagcagg 2100

aggaggatca agaagagaac ccgagagccg gcctggaccc tccggcggag gaggaggagt 2160

agctgacctg tttcctgaac tgcgccgggt gctgactagg tcttcgagtg gtcgggagag 2220

ggggattaag cgggagaggc atgatgagac taatcacaga actgaactga ctgtgggtct 2280

gatgagtcgc aagcgcccag aaacagtgtg gtggcatgag gtgcagtcga ctggcacaga 2340

tgaggtgtcg gtgatgcatg agaggttttc tctagaacaa gtcaagactt gttggttaga 2400

gcctgaggat gattgggagg tagccatcag gaattatgcc aagctggctc tgaggccaga 2460

caagaagtac aagattacta agctgataaa tatcagaaat gcctgctaca tctcagggaa 2520

tggggctgaa gtggagatct gtctccagga aagggtggct ttcagatgct gcatgatgaa 2580

tatgtacccg ggagtggtgg gcatggatgg ggttaccttt atgaacatga ggttcagggg 2640

agatgggtat aatggcacgg tctttatggc caataccaag ctgacagtcc atggctgctc 2700

cttctttggg tttaataaca cctgcatcga ggcctggggt caggtcggtg tgaggggctg 2760

cagtttttca gccaactgga tgggggtcgt gggcaggacc aagagtatgc tgtccgtgaa 2820

gaaatgcttg tttgagaggt gccacctggg ggtgatgagc gagggcgaag ccagaatccg 2880

ccactgcgcc tctaccgaga cgggctgctt tgtgctgtgc aagggcaatg ctaagatcaa 2940

gcataatatg atctgtggag cctcggacga gcgcggctac cagatgctga cctgcgccgg 3000

cgggaacagc catatgctgg ccaccgtaca tgtggcttcc catgctcgca agccctggcc 3060

cgagttcgag cacaatgtca tgaccaggtg caatatgcat ctggggtccc gccgaggcat 3120

gttcatgccc taccagtgca acctgaatta tgtgaaggtg ctgctggagc ccgatgccat 3180

gtccagagtg agcctgacgg gggtgtttga catgaatgtg gaggtgtgga agattctgag 3240

atatgatgaa tccaagacca ggtgccgagc ctgcgagtgc ggagggaagc atgccaggtt 3300

ccagcccgtg tgtgtggatg tgacggagga cctgcgaccc gatcatttgg tgttgccctg 3360

caccgggacg gagttcggtt ccagcgggga agaatctgac tagagtgagt agtgttctgg 3420

ggcgggggag gacctgcatg agggccagaa taactgaaat ctgtgctttt ctgtgtgttg 3480

cagcagcatg agcggaagcg gctcctttga gggaggggta ttcagccctt atctgacggg 3540

gcgtctcccc tcctgggcgg gagtgcgtca gaatgtgatg ggatccacgg tggacggccg 3600

gcccgtgcag cccgcgaact cttcaaccct gacctatgca accctgagct cttcgtcgtt 3660

ggacgcagct gccgccgcag ctgctgcatc tgccgccagc gccgtgcgcg gaatggccat 3720

gggcgccggc tactacggca ctctggtggc caactcgagt tccaccaata atcccgccag 3780

cctgaacgag gagaagctgt tgctgctgat ggcccagctc gaggccttga cccagcgcct 3840

gggcgagctg acccagcagg tggctcagct gcaggagcag acgcgggccg cggttgccac 3900

ggtgaaatcc aaataaaaaa tgaatcaata aataaacgga gacggttgtt gattttaaca 3960

cagagtctga atctttattt gatttttcgc gcgcggtagg ccctggacca ccggtctcga 4020

tcattgagca cccggtggat cttttccagg acccggtaga ggtgggcttg gatgttgagg 4080

tacatgggca tgagcccgtc ccgggggtgg aggtagctcc attgcagggc ctcgtgctcg 4140

ggggtggtgt tgtaaatcac ccagtcatag caggggcgca gggcatggtg ttgcacaata 4200

tctttgagga ggagactgat ggccacgggc agccctttgg tgtaggtgtt tacaaatctg 4260

ttgagctggg agggatgcat gcggggggag atgaggtgca tcttggcctg gatcttgaga 4320

ttggcgatgt taccgcccag atcccgcctg gggttcatgt tgtgcaggac caccagcacg 4380

gtgtatccgg tgcacttggg gaatttatca tgcaacttgg aagggaaggc gtgaaagaat 4440

ttggcgacgc ctttgtgccc gcccaggttt tccatgcact catccatgat gatggcgatg 4500

ggcccgtggg cggcggcctg ggcaaagacg tttcgggggt cggacacatc atagttgtgg 4560

tcctgggtga ggtcatcata ggccatttta atgaatttgg ggcggagggt gccggactgg 4620

gggacaaagg taccctcgat cccgggggcg tagttcccct cacagatctg catctcccag 4680

gctttgagct cggagggggg gatcatgtcc acctgcgggg cgataaagaa cacggtttcc 4740

ggggcggggg agatgagctg ggccgaaagc aagttccgga gcagctggga cttgccgcag 4800

ccggtggggc cgtagatgac cccgatgacc ggctgcaggt ggtagttgag ggagagacag 4860

ctgccgtcct cccggaggag gggggccacc tcgttcatca tctcgcgcac gtgcatgttc 4920

tcgcgcacca gttccgccag gaggcgctct ccccccaggg ataggagctc ctggagcgag 4980

gcgaagtttt tcagcggctt gagtccgtcg gccatgggca ttttggagag ggtttgttgc 5040

aagagttcca ggcggtccca gagctcggtg atgtgctcta cggcatctcg atccagcaga 5100

cctcctcgtt tcgcgggttg ggacggctgc gggagtaggg caccagacga tgggcgtcca 5160

gcgcagccag ggtccggtcc ttccagggtc gcagcgtccg cgtcagggtg gtctccgtca 5220

cggtgaaggg gtgcgcgccg ggctgggcgc ttgcgagggt gcgcttcagg ctcatccggc 5280

tggtcgaaaa ccgctcccga tcggcgccct gcgcgtcggc caggtagcaa ttgaccatga 5340

gttcgtagtt gagcgcctcg gccgcgtggc ctttggcgcg gagcttacct ttggaagtct 5400

gcccgcaggc gggacagagg agggacttga gggcgtagag cttgggggcg aggaagacgg 5460

actcgggggc gtaggcgtcc gcgccgcagt gggcgcagac ggtctcgcac tccacgagcc 5520

aggtgaggtc gggctggtcg gggtcaaaaa ccagtttccc gccgttcttt ttgatgcgtt 5580

tcttaccttt ggtctccatg agctcgtgtc cccgctgggt gacaaagagg ctgtccgtgt 5640

ccccgtagac cgactttatg ggccggtcct cgagcggtgt gccgcggtcc tcctcgtaga 5700

ggaaccccgc ccactccgag acgaaagccc gggtccaggc cagcacgaag gaggccacgt 5760

gggacgggta gcggtcgttg tccaccagcg ggtccacctt ttccagggta tgcaaacaca 5820

tgtccccctc gtccacatcc aggaaggtga ttggcttgta agtgtaggcc acgtgaccgg 5880

gggtcccggc cgggggggta taaaagggtg cgggtccctg ctcgtcctca ctgtcttccg 5940

gatcgctgtc caggagcgcc agctgttggg gtaggtattc cctctcgaag gcgggcatga 6000

cctcggcact caggttgtca gtttctagaa acgaggagga tttgatattg acggtgccgg 6060

cggagatgcc tttcaagagc ccctcgtcca tctggtcaga aaagacgatc tttttgttgt 6120

cgagcttggt ggcgaaggag ccgtagaggg cgttggagag gagcttggcg atggagcgca 6180

tggtctggtt tttttccttg tcggcgcgct ccttggcggc gatgttgagc tgcacgtact 6240

cgcgcgccac gcacttccat tcggggaaga cggtggtcag ctcgtcgggc acgattctga 6300

cctgccagcc ccgattatgc agggtgatga ggtccacact ggtggccacc tcgccgcgca 6360

ggggctcatt agtccagcag aggcgtccgc ccttgcgcga gcagaagggg ggcagggggt 6420

ccagcatgac ctcgtcgggg gggtcggcat cgatggtgaa gatgccgggc aggaggtcgg 6480

ggtcaaagta gctgatggaa gtggccagat cgtccagggc agcttgccat tcgcgcacgg 6540

ccagcgcgcg ctcgtaggga ctgaggggcg tgccccaggg catgggatgg gtaagcgcgg 6600

aggcgtacat gccgcagatg tcgtagacgt agaggggctc ctcgaggatg ccgatgtagg 6660

tggggtagca gcgccccccg cggatgctgg cgcgcacgta gtcatacagc tcgtgcgagg 6720

gggcgaggag ccccgggccc aggttggtgc gactgggctt ttcggcgcgg tagacgatct 6780

ggcggaaaat ggcatgcgag ttggaggaga tggtgggcct ttggaagatg ttgaagtggg 6840

cgtggggcag tccgaccgag tcgcggatga agtgggcgta ggagtcttgc agcttggcga 6900

cgagctcggc ggtgactagg acgtccagag cgcagtagtc gagggtctcc tggatgatgt 6960

catacttgag ctgtcccttt tgtttccaca gctcgcggtt gagaaggaac tcttcgcggt 7020

ccttccagta ctcttcgagg gggaacccgt cctgatctgc acggtaagag cctagcatgt 7080

agaactggtt gacggccttg taggcgcagc agcccttctc cacggggagg gcgtaggcct 7140

gggcggcctt gcgcagggag gtgtgcgtga gggcgaaagt gtccctgacc atgaccttga 7200

ggaactggtg cttgaagtcg atatcgtcgc agcccccctg ctcccagagc tggaagtccg 7260

tgcgcttctt gtaggcgggg ttgggcaaag cgaaagtaac atcgttgaag aggatcttgc 7320

ccgcgcgggg cataaagttg cgagtgatgc ggaaaggttg gggcacctcg gcccggttgt 7380

tgatgacctg ggcggcgagc acgatctcgt cgaagccgtt gatgttgtgg cccacgatgt 7440

agagttccac gaatcgcgga cggcccttga cgtggggcag tttcttgagc tcctcgtagg 7500

tgagctcgtc ggggtcgctg agcccgtgct gctcgagcgc ccagtcggcg agatgggggt 7560

tggcgcggag gaaggaagtc cagagatcca cggccagggc ggtttgcaga cggtcccggt 7620

actgacggaa ctgctgcccg acggccattt tttcgggggt gacgcagtag aaggtgcggg 7680

ggtccccgtg ccagcgatcc catttgagct ggagggcgag atcgagggcg agctcgacga 7740

gccggtcgtc cccggagagt ttcatgacca gcatgaaggg gacgagctgc ttgccgaagg 7800

accccatcca ggtgtaggtt tccacatcgt aggtgaggaa gagcctttcg gtgcgaggat 7860

gcgagccgat ggggaagaac tggatctcct gccaccaatt ggaggaatgg ctgttgatgt 7920

gatggaagta gaaatgccga cggcgcgccg aacactcgtg cttgtgttta tacaagcggc 7980

cacagtgctc gcaacgctgc acgggatgca cgtgctgcac gagctgtacc tgagttcctt 8040

tgacgaggaa tttcagtggg aagtggagtc gtggcgcctg catctcgtgc tgtactacgt 8100

cgtggtggtc ggcctggccc tcttctgcct cgatggtggt catgctgacg agcccgcgcg 8160

ggaggcaggt ccagacctcg gcgcgagcgg gtcggagagc gaggacgagg gcgcgcaggc 8220

cggagctgtc cagggtcctg agacgctgcg gagtcaggtc agtgggcagc ggcggcgcgc 8280

ggttgacttg caggagtttt tccagggcgc gcgggaggtc cagatggtac ttgatctcca 8340

ccgcgccatt ggtggcgacg tcgatggctt gcagggtccc gtgcccctgg ggtgtgacca 8400

ccgtcccccg tttcttcttg ggcggctggg gcgacggggg cggtgcctct tccatggtta 8460

gaagcggcgg cgaggacgcg cgccgggcgg caggggcggc tcggggcccg gaggcagggg 8520

cggcaggggc acgtcggcgc cgcgcgcggg taggttctgg tactgcgccc ggagaagact 8580

ggcgtgagcg acgacgcgac ggttgacgtc ctggatctga cgcctctggg tgaaggccac 8640

gggacccgtg agtttgaacc tgaaagagag ttcgacagaa tcaatctcgg tatcgttgac 8700

ggcggcctgc cgcaggatct cttgcacgtc gcccgagttg tcctggtagg cgatctcggt 8760

catgaactgc tcgatctcct cctcttgaag gtctccgcgg ccggcgcgct ccacggtggc 8820

cgcgaggtcg ttggagatgc ggcccatgag ctgcgagaag gcgttcatgc ccgcctcgtt 8880

ccagacgcgg ctgtagacca cgacgccctc gggatcgccg gcgcgcatga ccacctgggc 8940

gaggttgagc tccacgtggc gcgtgaagac cgcgtagttg cagaggcgct ggtagaggta 9000

gttgagcgtg gtggcgatgt gctcggtgac gaagaaatac atgatccagc ggcggagcgg 9060

catctcgctg acgtcgccca gcgcctccaa acgttccatg gcctcgtaaa agtccacggc 9120

gaagttgaaa aactgggagt tgcgcgccga gacggtcaac tcctcctcca gaagacggat 9180

gagctcggcg atggtggcgc gcacctcgcg ctcgaaggcc cccgggagtt cctccacttc 9240

ctcttcttcc tcctccacta acatctcttc tacttcctcc tcaggcggca gtggtggcgg 9300

gggagggggc ctgcgtcgcc ggcggcgcac gggcagacgg tcgatgaagc gctcgatggt 9360

ctcgccgcgc cggcgtcgca tggtctcggt gacggcgcgc ccgtcctcgc ggggccgcag 9420

cgtgaagacg ccgccgcgca tctccaggtg gccggggggg tccccgttgg gcagggagag 9480

ggcgctgacg atgcatctta tcaattgccc cgtagggact ccgcgcaagg acctgagcgt 9540

ctcgagatcc acgggatctg aaaaccgctg aacgaaggct tcgagccagt cgcagtcgca 9600

aggtaggctg agcacggttt cttctggcgg gtcatgttgg ttgggagcgg ggcgggcgat 9660

gctgctggtg atgaagttga aataggcggt tctgagacgg cggatggtgg cgaggagcac 9720

caggtctttg ggcccggctt gctggatgcg cagacggtcg gccatgcccc aggcgtggtc 9780

ctgacacctg gccaggtcct tgtagtagtc ctgcatgagc cgctccacgg gcacctcctc 9840

ctcgcccgcg cggccgtgca tgcgcgtgag cccgaagccg cgctggggct ggacgagcgc 9900

caggtcggcg acgacgcgct cggcgaggat ggcttgctgg atctgggtga gggtggtctg 9960

gaagtcatca aagtcgacga agcggtggta ggctccggtg ttgatggtgt aggagcagtt 10020

ggccatgacg gaccagttga cggtctggtg gcccggacgc acgagctcgt ggtacttgag 10080

gcgcgagtag gcgcgcgtgt cgaagatgta gtcgttgcag gtgcgcacca ggtactggta 10140

gccgatgagg aagtgcggcg gcggctggcg gtagagcggc catcgctcgg tggcgggggc 10200

gccgggcgcg aggtcctcga gcatggtgcg gtggtagccg tagatgtacc tggacatcca 10260

ggtgatgccg gcggcggtgg tggaggcgcg cgggaactcg cggacgcggt tccagatgtt 10320

gcgcagcggc aggaagtagt tcatggtggg cacggtctgg cccgtgaggc gcgcgcagtc 10380

gtggatgctc tatacgggca aaaacgaaag cggtcagcgg ctcgactccg tggcctggag 10440

gctaagcgaa cgggttgggc tgcgcgtgta ccccggttcg aatctcgaat caggctggag 10500

ccgcagctaa cgtggtattg gcactcccgt ctcgacccaa gcctgcacca accctccagg 10560

atacggaggc gggtcgtttt gcaacttttt tttggaggcc ggatgagact agtaagcgcg 10620

gaaagcggcc gaccgcgatg gctcgctgcc gtagtctgga gaagaatcgc cagggttgcg 10680

ttgcggtgtg ccccggttcg aggccggccg gattccgcgg ctaacgaggg cgtggctgcc 10740

ccgtcgtttc caagacccca tagccagccg acttctccag ttacggagcg agcccctctt 10800

ttgttttgtt tgtttttgcc agatgcatcc cgtactgcgg cagatgcgcc cccaccaccc 10860

tccaccgcaa caacagcccc ctccacagcc ggcgcttctg cccccgcccc agcagcaact 10920

tccagccacg accgccgcgg ccgccgtgag cggggctgga cagagttatg atcaccagct 10980

ggccttggaa gagggcgagg ggctggcgcg cctgggggcg tcgtcgccgg agcggcaccc 11040

gcgcgtgcag atgaaaaggg acgctcgcga ggcctacgtg cccaagcaga acctgttcag 11100

agacaggagc ggcgaggagc ccgaggagat gcgcgcggcc cggttccacg cggggcggga 11160

gctgcggcgc ggcctggacc gaaagagggt gctgagggac gaggatttcg aggcggacga 11220

gctgacgggg atcagccccg cgcgcgcgca cgtggccgcg gccaacctgg tcacggcgta 11280

cgagcagacc gtgaaggagg agagcaactt ccaaaaatcc ttcaacaacc acgtgcgcac 11340

cctgatcgcg cgcgaggagg tgaccctggg cctgatgcac ctgtgggacc tgctggaggc 11400

catcgtgcag aaccccacca gcaagccgct gacggcgcag ctgttcctgg tggtgcagca 11460

tagtcgggac aacgaagcgt tcagggaggc gctgctgaat atcaccgagc ccgagggccg 11520

ctggctcctg gacctggtga acattctgca gagcatcgtg gtgcaggagc gcgggctgcc 11580

gctgtccgag aagctggcgg ccatcaactt ctcggtgctg agtttgggca agtactacgc 11640

taggaagatc tacaagaccc cgtacgtgcc catagacaag gaggtgaaga tcgacgggtt 11700

ttacatgcgc atgaccctga aagtgctgac cctgagcgac gatctggggg tgtaccgcaa 11760

cgacaggatg caccgtgcgg tgagcgccag caggcggcgc gagctgagcg accaggagct 11820

gatgcatagt ctgcagcggg ccctgaccgg ggccgggacc gagggggaga gctactttga 11880

catgggcgcg gacctgcact ggcagcccag ccgccgggcc ttggaggcgg cggcaggacc 11940

ctacgtagaa gaggtggacg atgaggtgga cgaggagggc gagtacctgg aagactgatg 12000

gcgcgaccgt atttttgcta gatgcaacaa caacagccac ctcctgatcc cgcgatgcgg 12060

gcggcgctgc agagccagcc gtccggcatt aactcctcgg acgattggac ccaggccatg 12120

caacgcatca tggcgctgac gacccgcaac cccgaagcct ttagacagca gccccaggcc 12180

aaccggctct cggccatcct ggaggccgtg gtgccctcgc gctccaaccc cacgcacgag 12240

aaggtcctgg ccatcgtgaa cgcgctggtg gagaacaagg ccatccgcgg cgacgaggcc 12300

ggcctggtgt acaacgcgct gctggagcgc gtggcccgct acaacagcac caacgtgcag 12360

accaacctgg accgcatggt gaccgacgtg cgcgaggccg tggcccagcg cgagcggttc 12420

caccgcgagt ccaacctggg atccatggtg gcgctgaacg ccttcctcag cacccagccc 12480

gccaacgtgc cccggggcca ggaggactac accaacttca tcagcgccct gcgcctgatg 12540

gtgaccgagg tgccccagag cgaggtgtac cagtccgggc cggactactt cttccagacc 12600

agtcgccagg gcttgcagac cgtgaacctg agccaggctt tcaagaactt gcagggcctg 12660

tggggcgtgc aggccccggt cggggaccgc gcgacggtgt cgagcctgct gacgccgaac 12720

tcgcgcctgc tgctgctgct ggtggccccc ttcacggaca gcggcagcat caaccgcaac 12780

tcgtacctgg gctacctgat taacctgtac cgcgaggcca tcggccaggc gcacgtggac 12840

gagcagacct accaggagat cacccacgtg agccgcgccc tgggccagga cgacccgggc 12900

aacctggaag ccaccctgaa ctttttgctg accaaccggt cgcagaagat cccgccccag 12960

tacgcgctca gcaccgagga ggagcgcatc ctgcgttacg tgcagcagag cgtgggcctg 13020

ttcctgatgc aggagggggc cacccccagc gccgcgctcg acatgaccgc gcgcaacatg 13080

gagcccagca tgtacgccag caaccgcccg ttcatcaata aactgatgga ctacttgcat 13140

cgggcggccg ccatgaactc tgactatttc accaacgcca tcctgaatcc ccactggctc 13200

ccgccgccgg ggttctacac gggcgagtac gacatgcccg accccaatga cgggttcctg 13260

tgggacgatg tggacagcag cgtgttctcc ccccgaccgg gtgctaacga gcgccccttg 13320

tggaagaagg aaggcagcga ccgacgcccg tcctcggcgc tgtccggccg cgagggtgct 13380

gccgcggcgg tgcccgaggc cgccagtcct ttcccgagct tgcccttctc gctgaacagt 13440

atccgcagca gcgagctggg caggatcacg cgcccgcgct tgctgggcga agaggagtac 13500

ttgaatgact cgctgttgag acccgagcgg gagaagaact tccccaataa cgggatagaa 13560

agcctggtgg acaagatgag ccgctggaag acgtatgcgc aggagcacag ggacgatccc 13620

cgggcgtcgc agggggccac gagccggggc agcgccgccc gtaaacgccg gtggcacgac 13680

aggcagcggg gacagatgtg ggacgatgag gactccgccg acgacagcag cgtgttggac 13740

ttgggtggga gtggtaaccc gttcgctcac ctgcgccccc gtatcgggcg catgatgtaa 13800

gagaaaccga aaataaatga tactcaccaa ggccatggcg accagcgtgc gttcgtttct 13860

tctctgttgt tgttgtatct agtatgatga ggcgtgcgta cccggagggt cctcctccct 13920

cgtacgagag cgtgatgcag caggcgatgg cggcggcggc gatgcagccc ccgctggagg 13980

ctccttacgt gcccccgcgg tacctggcgc ctacggaggg gcggaacagc attcgttact 14040

cggagctggc acccttgtac gataccaccc ggttgtacct ggtggacaac aagtcggcgg 14100

acatcgcctc gctgaactac cagaacgacc acagcaactt cctgaccacc gtggtgcaga 14160

acaatgactt cacccccacg gaggccagca cccagaccat caactttgac gagcgctcgc 14220

ggtggggcgg ccagctgaaa accatcatgc acaccaacat gcccaacgtg aacgagttca 14280

tgtacagcaa caagttcaag gcgcgggtga tggtctcccg caagaccccc aatggggtga 14340

cagtgacaga ggattatgat ggtagtcagg atgagctgaa gtatgaatgg gtggaatttg 14400

agctgcccga aggcaacttc tcggtgacca tgaccatcga cctgatgaac aacgccatca 14460

tcgacaatta cttggcggtg gggcggcaga acggggtgct ggagagcgac atcggcgtga 14520

agttcgacac taggaacttc aggctgggct gggaccccgt gaccgagctg gtcatgcccg 14580

gggtgtacac caacgaggct ttccatcccg atattgtctt gctgcccggc tgcggggtgg 14640

acttcaccga gagccgcctc agcaacctgc tgggcattcg caagaggcag cccttccagg 14700

aaggcttcca gatcatgtac gaggatctgg aggggggcaa catccccgcg ctcctggatg 14760

tcgacgccta tgagaaaagc aaggaggatg cagcagctga agcaactgca gccgtagcta 14820

ccgcctctac cgaggtcagg ggcgataatt ttgcaagcgc cgcagcagtg gcagcggccg 14880

aggcggctga aaccgaaagt aagatagtca ttcagccggt ggagaaggat agcaagaaca 14940

ggagctacaa cgtactaccg gacaagataa acaccgccta ccgcagctgg tacctagcct 15000

acaactatgg cgaccccgag aagggcgtgc gctcctggac gctgctcacc acctcggacg 15060

tcacctgcgg cgtggagcaa gtctactggt cgctgcccga catgatgcaa gacccggtca 15120

ccttccgctc cacgcgtcaa gttagcaact acccggtggt gggcgccgag ctcctgcccg 15180

tctactccaa gagcttcttc aacgagcagg ccgtctactc gcagcagctg cgcgccttca 15240

cctcgcttac gcacgtcttc aaccgcttcc ccgagaacca gatcctcgtc cgcccgcccg 15300

cgcccaccat taccaccgtc agtgaaaacg ttcctgctct cacagatcac gggaccctgc 15360

cgctgcgcag cagtatccgg ggagtccagc gcgtgaccgt tactgacgcc agacgccgca 15420

cctgccccta cgtctacaag gccctgggca tagtcgcgcc gcgcgtcctc tcgagccgca 15480

ccttctaaat gtccattctc atctcgccca gtaataacac cggttggggc ctgcgcgcgc 15540

ccagcaagat gtacggaggc gctcgccaac gctccacgca acaccccgtg cgcgtgcgcg 15600

ggcacttccg cgctccctgg ggcgccctca agggccgcgt gcggtcgcgc accaccgtcg 15660

acgacgtgat cgaccaggtg gtggccgacg cgcgcaacta cacccccgcc gccgcgcccg 15720

tctccaccgt ggacgccgtc atcgacagcg tggtggcgga cgcgcgccgg tacgcccgcg 15780

ccaagagccg gcggcggcgc atcgcccggc ggcaccggag cacccccgcc atgcgcgcgg 15840

cgcgagcctt gctgcgcagg gccaggcgca cgggacgcag ggccatgctc agggcggcca 15900

gacgcgcggc ttcaggcgcc agcgccggca ggacccggag acgcgcggcc acggcggcgg 15960

cagcggccat cgccagcatg tcccgcccgc ggcgagggaa cgtgtactgg gtgcgcgacg 16020

ccgccaccgg tgtgcgcgtg cccgtgcgca cccgcccccc tcgcacttga agatgttcac 16080

ttcgcgatgt tgatgtgtcc cagcggcgag gaggatgtcc aagcgcaaat tcaaggaaga 16140

gatgctccag gtcatcgcgc ctgagatcta cggccctgcg gtggtgaagg aggaaagaaa 16200

gccccgcaaa atcaagcggg tcaaaaagga caaaaaggaa gaagaaagtg atgtggacgg 16260

attggtggag tttgtgcgcg agttcgcccc ccggcggcgc gtgcagtggc gcgggcggaa 16320

ggtgcaaccg gtgctgagac ccggcaccac cgtggtcttc acgcccggcg agcgctccgg 16380

caccgcttcc aagcgctcct acgacgaggt gtacggggat gatgatattc tggagcaggc 16440

ggccgagcgc ctgggcgagt ttgcttacgg caagcgcagc cgttccgcac cgaaggaaga 16500

ggcggtgtcc atcccgctgg accacggcaa ccccacgccg agcctcaagc ccgtgacctt 16560

gcagcaggtg ctgccgaccg cggcgccgcg ccgggggttc aagcgcgagg gcgaggatct 16620

gtaccccacc atgcagctga tggtgcccaa gcgccagaag ctggaagacg tgctggagac 16680

catgaaggtg gacccggacg tgcagcccga ggtcaaggtg cggcccatca agcaggtggc 16740

cccgggcctg ggcgtgcaga ccgtggacat caagattccc acggagccca tggaaacgca 16800

gaccgagccc atgatcaagc ccagcaccag caccatggag gtgcagacgg atccctggat 16860

gccatcggct cctagtcgaa gaccccggcg caagtacggc gcggccagcc tgctgatgcc 16920

caactacgcg ctgcatcctt ccatcatccc cacgccgggc taccgcggca cgcgcttcta 16980

ccgcggtcat accagcagcc gccgccgcaa gaccaccact cgccgccgcc gtcgccgcac 17040

cgccgctgca accacccctg ccgccctggt gcggagagtg taccgccgcg gccgcgcacc 17100

tctgaccctg ccgcgcgcgc gctaccaccc gagcatcgcc atttaaactt tcgccagctt 17160

tgcagatcaa tggccctcac atgccgcctt cgcgttccca ttacgggcta ccgaggaaga 17220

aaaccgcgcc gtagaaggct ggcggggaac gggatgcgtc gccaccacca ccggcggcgg 17280

cgcgccatca gcaagcggtt ggggggaggc ttcctgcccg cgctgatccc catcatcgcc 17340

gcggcgatcg gggcgatccc cggcattgct tccgtggcgg tgcaggcctc tcagcgccac 17400

tgagacacac ttggaaacat cttgtaataa acccatggac tctgacgctc ctggtcctgt 17460

gatgtgtttt cgtagacaga tggaagacat caatttttcg tccctggctc cgcgacacgg 17520

cacgcggccg ttcatgggca cctggagcga catcggcacc agccaactga acgggggcgc 17580

cttcaattgg agcagtctct ggagcgggct taagaatttc gggtccacgc ttaaaaccta 17640

tggcagcaag gcgtggaaca gcaccacagg gcaggcgctg agggataagc tgaaagagca 17700

gaacttccag cagaaggtgg tcgatgggct cgcctcgggc atcaacgggg tggtggacct 17760

ggccaaccag gccgtgcagc ggcagatcaa cagccgcctg gacccggtgc cgcccgccgg 17820

ctccgtggag atgccgcagg tggaggagga gctgcctccc ctggacaagc ggggcgagaa 17880

gcgaccccgc cccgatgcgg aggagacgct gctgacgcac acggacgagc cgcccccgta 17940

cgaggaggcg gtgaaactgg gtctgcccac cacgcggccc atcgcgcccc tggccaccgg 18000

ggtgctgaaa cccgaaaagc ccgcgaccct ggacttgcct cctccccagc cttcccgccc 18060

ctctacagtg gctaagcccc tgccgccggt ggccgtggcc cgcgcgcgac ccgggggcac 18120

cgcccgccct catgcgaact ggcagagcac tctgaacagc atcgtgggtc tgggagtgca 18180

gagtgtgaag cgccgccgct gctattaaac ctaccgtagc gcttaacttg cttgtctgtg 18240

tgtgtatgta ttatgtcgcc gccgccgctg tccaccagaa ggaggagtga agaggcgcgt 18300

cgccgagttg caagatggcc accccatcga tgctgcccca gtgggcgtac atgcacatcg 18360

ccggacagga cgcttcggag tacctgagtc cgggtctggt gcagtttgcc cgcgccacag 18420

acacctactt cagtctgggg aacaagttta ggaaccccac ggtggcgccc acgcacgatg 18480

tgaccaccga ccgcagccag cggctgacgc tgcgcttcgt gcccgtggac cgcgaggaca 18540

acacctactc gtacaaagtg cgctacacgc tggccgtggg cgacaaccgc gtgctggaca 18600

tggccagcac ctactttgac atccgcggcg tgctggatcg gggccctagc ttcaaaccct 18660

actccggcac cgcctacaac agtctggccc ccaagggagc acccaacact tgtcagtgga 18720

catataaagc cgatggtgaa actgccacag aaaaaaccta tacatatgga aatgcacccg 18780

tgcagggcat taacatcaca aaagatggta ttcaacttgg aactgacacc gatgatcagc 18840

caatctacgc agataaaacc tatcagcctg aacctcaagt gggtgatgct gaatggcatg 18900

acatcactgg tactgatgaa aagtatggag gcagagctct taagcctgat accaaaatga 18960

agccttgtta tggttctttt gccaagccta ctaataaaga aggaggtcag gcaaatgtga 19020

aaacaggaac aggcactact aaagaatatg acatagacat ggctttcttt gacaacagaa 19080

gtgcggctgc tgctggccta gctccagaaa ttgttttgta tactgaaaat gtggatttgg 19140

aaactccaga tacccatatt gtatacaaag caggcacaga tgacagcagc tcttctatta 19200

atttgggtca gcaagccatg cccaacagac ctaactacat tggtttcaga gacaacttta 19260

tcgggctcat gtactacaac agcactggca atatgggggt gctggccggt caggcttctc 19320

agctgaatgc tgtggttgac ttgcaagaca gaaacaccga gctgtcctac cagctcttgc 19380

ttgactctct gggtgacaga acccggtatt tcagtatgtg gaatcaggcg gtggacagct 19440

atgatcctga tgtgcgcatt attgaaaatc atggtgtgga ggatgaactt cccaactatt 19500

gtttccctct ggatgctgtt ggcagaacag atacttatca gggaattaag gctaatggaa 19560

ctgatcaaac cacatggacc aaagatgaca gtgtcaatga tgctaatgag ataggcaagg 19620

gtaatccatt cgccatggaa atcaacatcc aagccaacct gtggaggaac ttcctctacg 19680

ccaacgtggc cctgtacctg cccgactctt acaagtacac gccggccaat gttaccctgc 19740

ccaccaacac caacacctac gattacatga acggccgggt ggtggcgccc tcgctggtgg 19800

actcctacat caacatcggg gcgcgctggt cgctggatcc catggacaac gtgaacccct 19860

tcaaccacca ccgcaatgcg gggctgcgct accgctccat gctcctgggc aacgggcgct 19920

acgtgccctt ccacatccag gtgccccaga aatttttcgc catcaagagc ctcctgctcc 19980

tgcccgggtc ctacacctac gagtggaact tccgcaagga cgtcaacatg atcctgcaga 20040

gctccctcgg caacgacctg cgcacggacg gggcctccat ctccttcacc agcatcaacc 20100

tctacgccac cttcttcccc atggcgcaca acacggcctc cacgctcgag gccatgctgc 20160

gcaacgacac caacgaccag tccttcaacg actacctctc ggcggccaac atgctctacc 20220

ccatcccggc caacgccacc aacgtgccca tctccatccc ctcgcgcaac tgggccgcct 20280

tccgcggctg gtccttcacg cgtctcaaga ccaaggagac gccctcgctg ggctccgggt 20340

tcgaccccta cttcgtctac tcgggctcca tcccctacct cgacggcacc ttctacctca 20400

accacacctt caagaaggtc tccatcacct tcgactcctc cgtcagctgg cccggcaacg 20460

accggctcct gacgcccaac gagttcgaaa tcaagcgcac cgtcgacggc gagggctaca 20520

acgtggccca gtgcaacatg accaaggact ggttcctggt ccagatgctg gcccactaca 20580

acatcggcta ccagggcttc tacgtgcccg agggctacaa ggaccgcatg tactccttct 20640

tccgcaactt ccagcccatg agccgccagg tggtggacga ggtcaactac aaggactacc 20700

aggccgtcac cctggcctac cagcacaaca actcgggctt cgtcggctac ctcgcgccca 20760

ccatgcgcca gggccagccc taccccgcca actaccccta cccgctcatc ggcaagagcg 20820

ccgtcaccag cgtcacccag aaaaagttcc tctgcgacag ggtcatgtgg cgcatcccct 20880

tctccagcaa cttcatgtcc atgggcgcgc tcaccgacct cggccagaac atgctctatg 20940

ccaactccgc ccacgcgcta gacatgaatt tcgaagtcga ccccatggat gagtccaccc 21000

ttctctatgt tgtcttcgaa gtcttcgacg tcgtccgagt gcaccagccc caccgcggcg 21060

tcatcgaggc cgtctacctg cgcaccccct tctcggccgg taacgccacc acctaagctc 21120

ttgcttcttg caagccatgg ccgcgggctc cggcgagcag gagctcaggg ccatcatccg 21180

cgacctgggc tgcgggccct acttcctggg caccttcgat aagcgcttcc cgggattcat 21240

ggccccgcac aagctggcct gcgccatcgt caacacggcc ggccgcgaga ccgggggcga 21300

gcactggctg gccttcgcct ggaacccgcg ctcgaacacc tgctacctct tcgacccctt 21360

cgggttctcg gacgagcgcc tcaagcagat ctaccagttc gagtacgagg gcctgctgcg 21420

ccgcagcgcc ctggccaccg aggaccgctg cgtcaccctg gaaaagtcca cccagaccgt 21480

gcagggtccg cgctcggccg cctgcgggct cttctgctgc atgttcctgc acgccttcgt 21540

gcactggccc gaccgcccca tggacaagaa ccccaccatg aacttgctga cgggggtgcc 21600

caacggcatg ctccagtcgc cccaggtgga acccaccctg cgccgcaacc aggaggcgct 21660

ctaccgcttc ctcaactccc actccgccta ctttcgctcc caccgcgcgc gcatcgagaa 21720

ggccaccgcc ttcgaccgca tgaatcaaga catgtaaacc gtgtgtgtat gttaaatgtc 21780

tttaataaac agcactttca tgttacacat gcatctgaga tgatttattt agaaatcgaa 21840

agggttctgc cgggtctcgg catggcccgc gggcagggac acgttgcgga actggtactt 21900

ggccagccac ttgaactcgg ggatcagcag tttgggcagc ggggtgtcgg ggaaggagtc 21960

ggtccacagc ttccgcgtca gttgcagggc gcccagcagg tcgggcgcgg agatcttgaa 22020

atcgcagttg ggacccgcgt tctgcgcgcg ggagttgcgg tacacggggt tgcagcactg 22080

gaacaccatc agggccgggt gcttcacgct cgccagcacc gtcgcgtcgg tgatgctctc 22140

cacgtcgagg tcctcggcgt tggccatccc gaagggggtc atcttgcagg tctgccttcc 22200

catggtgggc acgcacccgg gcttgtggtt gcaatcgcag tgcaggggga tcagcatcat 22260

ctgggcctgg tcggcgttca tccccgggta catggccttc atgaaagcct ccaattgcct 22320

gaacgcctgc tgggccttgg ctccctcggt gaagaagacc ccgcaggact tgctagagaa 22380

ctggttggtg gcgcacccgg cgtcgtgcac gcagcagcgc gcgtcgttgt tggccagctg 22440

caccacgctg cgcccccagc ggttctgggt gatcttggcc cggtcggggt tctccttcag 22500

cgcgcgctgc ccgttctcgc tcgccacatc catctcgatc atgtgctcct tctggatcat 22560

ggtggtcccg tgcaggcacc gcagcttgcc ctcggcctcg gtgcacccgt gcagccacag 22620

cgcgcacccg gtgcactccc agttcttgtg ggcgatctgg gaatgcgcgt gcacgaagcc 22680

ctgcaggaag cggcccatca tggtggtcag ggtcttgttg ctagtgaagg tcagcggaat 22740

gccgcggtgc tcctcgttga tgtacaggtg gcagatgcgg cggtacacct cgccctgctc 22800

gggcatcagc tggaagttgg ctttcaggtc ggtctccacg cggtagcggt ccatcagcat 22860

agtcatgatt tccataccct tctcccaggc cgagacgatg ggcaggctca tagggttctt 22920

caccatcatc ttagcgctag cagccgcggc cagggggtcg ctctcgtcca gggtctcaaa 22980

gctccgcttg ccgtccttct cggtgatccg caccgggggg tagctgaagc ccacggccgc 23040

cagctcctcc tcggcctgtc tttcgtcctc gctgtcctgg ctgacgtcct gcaggaccac 23100

atgcttggtc ttgcggggtt tcttcttggg cggcagcggc ggcggagatg ttggagatgg 23160

cgagggggag cgcgagttct cgctcaccac tactatctct tcctcttctt ggtccgaggc 23220

cacgcggcgg taggtatgtc tcttcggggg cagaggcgga ggcgacgggc tctcgccgcc 23280

gcgacttggc ggatggctgg cagagcccct tccgcgttcg ggggtgcgct cccggcggcg 23340

ctctgactga cttcctccgc ggccggccat tgtgttctcc tagggaggaa caacaagcat 23400

ggagactcag ccatcgccaa cctcgccatc tgcccccacc gccgacgaga agcagcagca 23460

gcagaatgaa agcttaaccg ccccgccgcc cagccccgcc acctccgacg cggccgtccc 23520

agacatgcaa gagatggagg aatccatcga gattgacctg ggctatgtga cgcccgcgga 23580

gcacgaggag gagctggcag tgcgcttttc acaagaagag atacaccaag aacagccaga 23640

gcaggaagca gagaatgagc agagtcaggc tgggctcgag catgacggcg actacctcca 23700

cctgagcggg ggggaggacg cgctcatcaa gcatctggcc cggcaggcca ccatcgtcaa 23760

ggatgcgctg ctcgaccgca ccgaggtgcc cctcagcgtg gaggagctca gccgcgccta 23820

cgagttgaac ctcttctcgc cgcgcgtgcc ccccaagcgc cagcccaatg gcacctgcga 23880

gcccaacccg cgcctcaact tctacccggt cttcgcggtg cccgaggccc tggccaccta 23940

ccacatcttt ttcaagaacc aaaagatccc cgtctcctgc cgcgccaacc gcacccgcgc 24000

cgacgccctt ttcaacctgg gtcccggcgc ccgcctacct gatatcgcct ccttggaaga 24060

ggttcccaag atcttcgagg gtctgggcag cgacgagact cgggccgcga acgctctgca 24120

aggagaagga ggagagcatg agcaccacag cgccctggtc gagttggaag gcgacaacgc 24180

gcggctggcg gtgctcaaac gcacggtcga gctgacccat ttcgcctacc cggctctgaa 24240

cctgcccccc aaagtcatga gcgcggtcat ggaccaggtg ctcatcaagc gcgcgtcgcc 24300

catctccgag gacgagggca tgcaagactc cgaggagggc aagcccgtgg tcagcgacga 24360

gcagctggcc cggtggctgg gtcctaatgc tagtccccag agtttggaag agcggcgcaa 24420

actcatgatg gccgtggtcc tggtgaccgt ggagctggag tgcctgcgcc gcttcttcgc 24480

cgacgcggag accctgcgca aggtcgagga gaacctgcac tacctcttca ggcacgggtt 24540

cgtgcgccag gcctgcaaga tctccaacgt ggagctgacc aacctggtct cctacatggg 24600

catcttgcac gagaaccgcc tggggcagaa cgtgctgcac accaccctgc gcggggaggc 24660

ccggcgcgac tacatccgcg actgcgtcta cctctacctc tgccacacct ggcagacggg 24720

catgggcgtg tggcagcagt gtctggagga gcagaacctg aaagagctct gcaagctcct 24780

gcagaagaac ctcaagggtc tgtggaccgg gttcgacgag cgcaccaccg cctcggacct 24840

ggccgacctc attttccccg agcgcctcag gctgacgctg cgcaacggcc tgcccgactt 24900

tatgagccaa agcatgttgc aaaactttcg ctctttcatc ctcgaacgct ccggaatcct 24960

gcccgccacc tgctccgcgc tgccctcgga cttcgtgccg ctgaccttcc gcgagtgccc 25020

cccgccgctg tggagccact gctacctgct gcgcctggcc aactacctgg cctaccactc 25080

ggacgtgatc gaggacgtca gcggcgaggg cctgctcgag tgccactgcc gctgcaacct 25140

ctgcacgccg caccgctccc tggcctgcaa cccccagctg ctgagcgaga cccagatcat 25200

cggcaccttc gagttgcaag ggcccagcga aggcgagggt tcagccgcca aggggggtct 25260

gaaactcacc ccggggctgt ggacctcggc ctacttgcgc aagttcgtgc ccgaggacta 25320

ccatcccttc gagatcaggt tctacgagga ccaatcccat ccgcccaagg ccgagctgtc 25380

ggcctgcgtc atcacccagg gggcgatcct ggcccaattg caagccatcc agaaatcccg 25440

ccaagaattc ttgctgaaaa agggccgcgg ggtctacctc gacccccaga ccggtgagga 25500

gctcaacccc ggcttccccc aggatgcccc gaggaaacaa gaagctgaaa gtggagctgc 25560

cgcccgtgga ggatttggag gaagactggg agaacagcag tcaggcagag gaggaggaga 25620

tggaggaaga ctgggacagc actcaggcag aggaggacag cctgcaagac agtctggagg 25680

aagacgagga ggaggcagag gaggaggtgg aagaagcagc cgccgccaga ccgtcgtcct 25740

cggcggggga gaaagcaagc agcacggata ccatctccgc tccgggtcgg ggtcccgctc 25800

gaccacacag tagatgggac gagaccggac gattcccgaa ccccaccacc cagaccggta 25860

agaaggagcg gcagggatac aagtcctggc gggggcacaa aaacgccatc gtctcctgct 25920

tgcaggcctg cgggggcaac atctccttca cccggcgcta cctgctcttc caccgcgggg 25980

tgaactttcc ccgcaacatc ttgcattact accgtcacct ccacagcccc tactacttcc 26040

aagaagaggc agcagcagca gaaaaagacc agcagaaaac cagcagctag aaaatccaca 26100

gcggcggcag caggtggact gaggatcgcg gcgaacgagc cggcgcaaac ccgggagctg 26160

aggaaccgga tctttcccac cctctatgcc atcttccagc agagtcgggg gcaggagcag 26220

gaactgaaag tcaagaaccg ttctctgcgc tcgctcaccc gcagttgtct gtatcacaag 26280

agcgaagacc aacttcagcg cactctcgag gacgccgagg ctctcttcaa caagtactgc 26340

gcgctcactc ttaaagagta gcccgcgccc gcccagtcgc agaaaaaggc gggaattacg 26400

tcacctgtgc ccttcgccct agccgcctcc acccatcatc atgagcaaag agattcccac 26460

gccttacatg tggagctacc agccccagat gggcctggcc gccggtgccg cccaggacta 26520

ctccacccgc atgaattggc tcagcgccgg gcccgcgatg atctcacggg tgaatgacat 26580

ccgcgcccac cgaaaccaga tactcctaga acagtcagcg ctcaccgcca cgccccgcaa 26640

tcacctcaat ccgcgtaatt ggcccgccgc cctggtgtac caggaaattc cccagcccac 26700

gaccgtacta cttccgcgag acgcccaggc cgaagtccag ctgactaact caggtgtcca 26760

gctggcgggc ggcgccaccc tgtgtcgtca ccgccccgct cagggtataa agcggctggt 26820

gatccggggc agaggcacac agctcaacga cgaggtggtg agctcttcgc tgggtctgcg 26880

acctgacgga gtcttccaac tcgccggatc ggggagatct tccttcacgc ctcgtcaggc 26940

cgtcctgact ttggagagtt cgtcctcgca gccccgctcg ggtggcatcg gcactctcca 27000

gttcgtggag gagttcactc cctcggtcta cttcaacccc ttctccggct cccccggcca 27060

ctacccggac gagttcatcc cgaacttcga cgccatcagc gagtcggtgg acggctacga 27120

ttgaatgtcc catggtggcg cagctgacct agctcggctt cgacacctgg accactgccg 27180

ccgcttccgc tgcttcgctc gggatctcgc cgagtttgcc tactttgagc tgcccgagga 27240

gcaccctcag ggcccggccc acggagtgcg gatcgtcgtc gaagggggcc tcgactccca 27300

cctgcttcgg atcttcagcc agcgtccgat cctggtcgag cgcgagcaag gacagaccct 27360

tctgactctg tactgcatct gcaaccaccc cggcctgcat gaaagtcttt gttgtctgct 27420

gtgtactgag tataataaaa gctgagatca gcgactactc cggacttccg tgtgttcctg 27480

aatccatcaa ccagtctttg ttcttcaccg ggaacgagac cgagctccag ctccagtgta 27540

agccccacaa gaagtacctc acctggctgt tccagggctc cccgatcgcc gttgtcaacc 27600

actgcgacaa cgacggagtc ctgctgagcg gccctgccaa ccttactttt tccacccgca 27660

gaagcaagct ccagctcttc caacccttcc tccccgggac ctatcagtgc gtctcgggac 27720

cctgccatca caccttccac ctgatcccga ataccacagc gtcgctcccc gctactaaca 27780

accaaactaa cctccaccaa cgccaccgtc gcgacctttc tgaatctaat actaccaccc 27840

acaccggagg tgagctccga ggtcaaccaa cctctgggat ttactacggc ccctgggagg 27900

tggttgggtt aatagcgcta ggcctagttg cgggtgggct tttggttctc tgctacctat 27960

acctcccttg ctgttcgtac ttagtggtgc tgtgttgctg gtttaagaaa tggggaagat 28020

caccctagtg agctgcggtg cgctggtggc ggtgttgctt tcgattgtgg gactgggcgg 28080

tgcggctgta gtgaaggaga aggccgatcc ctgcttgcat ttcaatccca acaaatgcca 28140

gctgagtttt cagcccgatg gcaatcggtg cgcggtactg atcaagtgcg gatgggaatg 28200

cgagaacgtg agaatcgagt acaataacaa gactcggaac aatactctcg cgtccgtgtg 28260

gcagcccggg gaccccgagt ggtacaccgt ctctgtcccc ggtgctgacg gctccccgcg 28320

caccgtgaat aatactttca tttttgcgca catgtgcgac acggtcatgt ggatgagcaa 28380

gcagtacgat atgtggcccc ccacgaagga gaacatcgtg gtcttctcca tcgcttacag 28440

cctgtgcacg gcgctaatca ccgctatcgt gtgcctgagc attcacatgc tcatcgctat 28500

tcgccccaga aataatgccg aaaaagaaaa acagccataa cgtttttttt cacacctttt 28560

tcagaccatg gcctctgtta aatttttgct tttatttgcc agtctcattg ccgtcattca 28620

tggaatgagt aatgagaaaa ttactattta cactggcact aatcacacat tgaaaggtcc 28680

agaaaaagcc acagaagttt catggtattg ttattttaat gaatcagatg tatctactga 28740

actctgtgga aacaataaca aaaaaaatga gagcattact ctcatcaagt ttcaatgtgg 28800

atctgactta accctaatta acatcactag agactatgta ggtatgtatt atggaactac 28860

agcaggcatt tcggacatgg aattttatca agtttctgtg tctgaaccca ccacgcctag 28920

aatgaccaca accacaaaaa ctacacctgt taccactatg cagctcacta ccaataacat 28980

ttttgccatg cgtcaaatgg tcaacaatag cactcaaccc accccaccca gtgaggaaat 29040

tcccaaatcc atgattggca ttattgttgc tgtagtggtg tgcatgttga tcatcgcctt 29100

gtgcatggtg tactatgcct tctgctacag aaagcacaga ctgaacgaca agctggaaca 29160

cttactaagt gttgaatttt aattttttag aaccatgaag atcctaggcc ttttaatttt 29220

ttctatcatt acctctgctc tatgcaattc tgacaatgag gacgttactg tcgttgtcgg 29280

atcaaattat acactgaaag gtccagcgaa gggtatgctt tcgtggtatt gctattttgg 29340

atctgacact acagaaactg aattatgcaa tcttaagaat ggcaaaattc aaaattctaa 29400

aattaacaat tatatatgca atggtactga tctgatactc ctcaatatca cgaaatcata 29460

tgctggcagt tacacctgcc ctggagatga tgctgacagt atgatttttt acaaagtaac 29520

tgttgttgat cccactactc cacctccacc caccacaact actcacacca cacacacaga 29580

tcaaaccgca gcagaggagg cagcaaagtt agccttgcag gtccaagaca gttcatttgt 29640

tggcattacc cctacacctg atcagcggtg tccggggctg ctagtcagcg gcattgtcgg 29700

tgtgctttcg ggattagcag tcataatcat ctgcatgttc atttttgctt gctgctatag 29760

aaggctttac cgacaaaaat cagacccact gctgaacctc tatgtttaat tttttccaga 29820

gtcatgaagg cagttagcgc tctagttttt tgttctttga ttggcattgt tttttgcaat 29880

cctattccta aagttagctt tattaaagat gtgaatgtta ctgagggggg caatgtgaca 29940

ctggtaggtg tagagggtgc tgaaaacacc acctggacaa aataccacct caatgggtgg 30000

aaagatattt gcaattggag tgtattagtt tatacatgtg agggagttaa tcttaccatt 30060

gtcaatgcca cctcagctca aaatggtaga attcaaggac aaagtgtcag tgtatctaat 30120

gggtatttta cccaacatac ttttatctat gacgttaaag tcataccact gcctacgcct 30180

agcccaccta gcactaccac acagacaacc cacactacac agacaaccac atacagtaca 30240

ttaaatcagc ctaccaccac tacagcagca gaggttgcca gctcgtctgg ggtccgagtg 30300

gcatttttga tgtgggcccc atctagcagt cccactgcta gtaccaatga gcagactact 30360

gaatttttgt ccactgtcga gagccacacc acagctacct ccagtgcctt ctctagcacc 30420

gccaatctct cctcgctttc ctctacacca atcagtcccg ctactactcc tagccccgct 30480

cctcttccca ctcccctgaa gcaaacagac ggcggcatgc aatggcagat caccctgctc 30540

attgtgatcg ggttggtcat cctggccgtg ttgctctact acatcttctg ccgccgcatt 30600

cccaacgcgc accgcaagcc ggtctacaag cccatcattg tcgggcagcc ggagccgctt 30660

caggtggaag ggggtctaag gaatcttctc ttctctttta cagtatggtg attgaactat 30720

gattcctaga caattcttga tcactattct tatctgcctc ctccaagtct gtgccaccct 30780

cgctctggtg gccaacgcca gtccagactg tattgggccc ttcgcctcct acgtgctctt 30840

tgccttcacc acctgcatct gctgctgtag catagtctgc ctgcttatca ccttcttcca 30900

gttcattgac tggatctttg tgcgcatcgc ctacctgcgc caccaccccc agtaccgcga 30960

ccagcgagtg gcgcggctgc tcaggctcct ctgataagca tgcgggctct gctacttctc 31020

gcgcttctgc tgttagtgct cccccgtccc gtcgaccccc ggtcccccac ccagtccccc 31080

gaggaggtcc gcaaatgcaa attccaagaa ccctggaaat tcctcaaatg ctaccgccaa 31140

aaatcagaca tgcatcccag ctggatcatg atcattggga tcgtgaacat tctggcctgc 31200

accctcatct cctttgtgat ttacccctgc tttgactttg gttggaactc gccagaggcg 31260

ctctatctcc cgcctgaacc tgacacacca ccacagcaac ctcaggcaca cgcactacca 31320

ccactacagc ctaggccaca atacatgccc atattagact atgaggccga gccacagcga 31380

cccatgctcc ccgctattag ttacttcaat ctaaccggcg gagatgactg acccactggc 31440

caacaacaac gtcaacgacc ttctcctgga catggacggc cgcgcctcgg agcagcgact 31500

cgcccaactt cgcattcgcc agcagcagga gagagccgtc aaggagctgc aggatgcggt 31560

ggccatccac cagtgcaaga gaggcatctt ctgcctggtg aaacaggcca agatctccta 31620

cgaggtcact ccaaacgacc atcgcctctc ctacgagctc ctgcagcagc gccagaagtt 31680

cacctgcctg gtcggagtca accccatcgt catcacccag cagtctggcg ataccaaggg 31740

gtgcatccac tgctcctgcg actcccccga ctgcgtccac actctgatca agaccctctg 31800

cggcctccgc gacctcctcc ccatgaacta atcaccccct tatccagtga aataaagatc 31860

atattgatga tgattttaca gaaataaaaa ataatcattt gatttgaaat aaagatacaa 31920

tcatattgat gatttgagtt taacaaaaaa ataaagaatc acttacttga aatctgatac 31980

caggtctctg tccatgtttt ctgccaacac cacttcactc ccctcttccc agctctggta 32040

ctgcaggccc cggcgggctg caaacttcct ccacacgctg aaggggatgt caaattcctc 32100

ctgtccctca atcttcattt tatcttctat cagatgtcca aaaagcgcgt ccgggtggat 32160

gatgacttcg accccgtcta cccctacgat gcagacaacg caccgaccgt gcccttcatc 32220

aaccccccct tcgtctcttc agatggattc caagagaagc ccctgggggt gttgtccctg 32280

cgactggccg accccgtcac caccaagaac ggggaaatca ccctcaagct gggagagggg 32340

gtggacctcg attcctcggg aaaactcatc tccaacacgg ccaccaaggc cgccgcccct 32400

ctcagttttt ccaacaacac catttccctt aacatggatc acccctttta cactaaagat 32460

ggaaaattat ccttacaagt ttctccacca ttaaatatac tgagaacaag cattctaaac 32520

acactagctt taggttttgg atcaggttta ggactccgtg gctctgcctt ggcagtacag 32580

ttagtctctc cacttacatt tgatactgat ggaaacataa agcttacctt agacagaggt 32640

ttgcatgtta caacaggaga tgcaattgaa agcaacataa gctgggctaa aggtttaaaa 32700

tttgaagatg gagccatagc aaccaacatt ggaaatgggt tagagtttgg aagcagtagt 32760

acagaaacag gtgttgatga tgcttaccca atccaagtta aacttggatc tggccttagc 32820

tttgacagta caggagccat aatggctggt aacaaagaag acgataaact cactttgtgg 32880

acaacacctg atccatcacc aaactgtcaa atactcgcag aaaatgatgc aaaactaaca 32940

ctttgcttga ctaaatgtgg tagtcaaata ctggccactg tgtcagtctt agttgtagga 33000

agtggaaacc taaaccccat tactggcacc gtaagcagtg ctcaggtgtt tctacgtttt 33060

gatgcaaacg gtgttctttt aacagaacat tctacactaa aaaaatactg ggggtatagg 33120

cagggagata gcatagatgg cactccatat accaatgctg taggattcat gcccaattta 33180

aaagcttatc caaagtcaca aagttctact actaaaaata atatagtagg gcaagtatac 33240

atgaatggag atgtttcaaa acctatgctt ctcactataa ccctcaatgg tactgatgac 33300

agcaacagta catattcaat gtcattttca tacacctgga ctaatggaag ctatgttgga 33360

gcaacatttg gggctaactc ttataccttc tcatacatcg cccaagaatg aacactgtat 33420

cccaccctgc atgccaaccc ttcccacccc actctgtgga acaaactctg aaacacaaaa 33480

taaaataaag ttcaagtgtt ttattgattc aacagtttta caggattcga gcagttattt 33540

ttcctccacc ctcccaggac atggaataca ccaccctctc cccccgcaca gccttgaaca 33600

tctgaatgcc attggtgatg gacatgcttt tggtctccac gttccacaca gtttcagagc 33660

gagccagtct cgggtcggtc agggagatga aaccctccgg gcactcccgc atctgcacct 33720

cacagctcaa cagctgagga ttgtcctcgg tggtcgggat cacggttatc tggaagaagc 33780

agaagagcgg cggtgggaat catagtccgc gaacgggatc ggccggtggt gtcgcatcag 33840

gccccgcagc agtcgctgcc gccgccgctc cgtcaagctg ctgctcaggg ggtccgggtc 33900

cagggactcc ctcagcatga tgcccacggc cctcagcatc agtcgtctgg tgcggcgggc 33960

gcagcagcgc atgcggatct cgctcaggtc gctgcagtac gtgcaacaca gaaccaccag 34020

gttgttcaac agtccatagt tcaacacgct ccagccgaaa ctcatcgcgg gaaggatgct 34080

acccacgtgg ccgtcgtacc agatcctcag gtaaatcaag tggtgccccc tccagaacac 34140

gctgcccacg tacatgatct ccttgggcat gtggcggttc accacctccc ggtaccacat 34200

caccctctgg ttgaacatgc agccccggat gatcctgcgg aaccacaggg ccagcaccgc 34260

cccgcccgcc atgcagcgaa gagaccccgg gtcccggcaa tggcaatgga ggacccaccg 34320

ctcgtacccg tggatcatct gggagctgaa caagtctatg ttggcacagc acaggcatat 34380

gctcatgcat ctcttcagca ctctcaactc ctcgggggtc aaaaccatat cccagggcac 34440

ggggaactct tgcaggacag cgaaccccgc agaacagggc aatcctcgca cagaacttac 34500

attgtgcatg gacagggtat cgcaatcagg cagcaccggg tgatcctcca ccagagaagc 34560

gcgggtctcg gtctcctcac agcgtggtaa gggggccggc cgatacgggt gatggcggga 34620

cgcggctgat cgtgttcgcg accgtgtcat gatgcagttg ctttcggaca ttttcgtact 34680

tgctgtagca gaacctggtc cgggcgctgc acaccgatcg ccggcggcgg tctcggcgct 34740

tggaacgctc ggtgttgaaa ttgtaaaaca gccactctct cagaccgtgc agcagatcta 34800

gggcctcagg agtgatgaag atcccatcat gcctgatggc tctgatcaca tcgaccaccg 34860

tggaatgggc cagacccagc cagatgatgc aattttgttg ggtttcggtg acggcggggg 34920

agggaagaac aggaagaacc atgattaact tttaatccaa acggtctcgg agtacttcaa 34980

aatgaagatc gcggagatgg cacctctcgc ccccgctgtg ttggtggaaa ataacagcca 35040

ggtcaaaggt gatacggttc tcgagatgtt ccacggtggc ttccagcaaa gcctccacgc 35100

gcacatccag aaacaagaca atagcgaaag cgggagggtt ctctaattcc tcaatcatca 35160

tgttacactc ctgcaccatc cccagataat tttcattttt ccagccttga atgattcgaa 35220

ctagttcgtg aggtaaatcc aagccagcca tgataaagag ctcgcgcaga gcgccctcca 35280

ccggcattct taagcacacc ctcataattc caagatattc tgctcctggt tcacctgcag 35340

cagattgaca agcggaatat caaaatctct gccgcgatcc ctgagctcct ccctcagcaa 35400

taactgtaag tactctttca tatcctctcc gaaattttta gccataggac caccaggaat 35460

aagattaggg caagccacag tacagataaa ccgaagtcct ccccagtgag cattgccaaa 35520

tgcaagactg ctataagcat gctggctaga cccggtgata tcttccagat aactggacag 35580

aaaatcgccc aggcaatttt taagaaaatc aacaaaagaa aaatcctcca ggtggacgtt 35640

tagagcctcg ggaacaacga tgaagtaaat gcaagcggtg cgttccagca tggttagtta 35700

gctgatctgt agaaaaaaca aaaatgaaca ttaaaccatg ctagcctggc gaacaggtgg 35760

gtaaatcgtt ctctccagca ccaggcaggc cacggggtct ccggcgcgac cctcgtaaaa 35820

attgtcgcta tgattgaaaa ccatcacaga gagacgttcc cggtggccgg cgtgaatgat 35880

tcgacaagat gaatacaccc ccggaacatt ggcgtccgcg agtgaaaaaa agcgcccgag 35940

gaagcaataa ggcactacaa tgctcagtct caagtccagc aaagcgatgc catgcggatg 36000

aagcacaaaa ttctcaggtg cgtacaaaat gtaattactc ccctcctgca caggcagcaa 36060

agcccccgat ccctccaggt acacatacaa agcctcagcg tccatagctt accgagcagc 36120

agcacacaac aggcgcaaga gtcagagaaa ggctgagctc taacctgtcc acccgctctc 36180

tgctcaatat atagcccaga tctacactga cgtaaaggcc aaagtctaaa aatacccgcc 36240

aaataatcac acacgcccag cacacgccca gaaaccggtg acacactcaa aaaaatacgc 36300

gcacttcctc aaacgcccaa aactgccgtc atttccgggt tcccacgcta cgtcatcaaa 36360

acacgacttt caaattccgt cgaccgttaa aaacgtcacc cgccccgccc ctaacggtcg 36420

cccgtctctc agccaatcag cgccccgcat ccccaaattc aaacacctca tttgcatatt 36480

aacgcgcaca aaaagtttga ggtatattat tgatgatgg 36519

<210> 3

<211> 2664

<212> DNA

<213> Pan troglodytes

<400> 3

atgagacatt tcacctacga tggcggtgtg ctcaccggcc agctggctgc tgaggtcctg 60

gacaccctga tcgaggaggt attggccgat aattatcctc cctcgactcc ttttgagcca 120

cctacacttc acgaactata cgatctggat gtggtggggc ccagcgatcc gaacgagcag 180

gcggtttcca gtttttttcc agagtccatg ttgttggcca gccaggaggg ggtcgaactt 240

gagacccctc ctccgatcgt ggattccccc gatccgccgc agctgactag gcagcccgag 300

cgctgtgcgg gacctgagac tatgccccag ctgctacctg aggtgatcga tctcacctgt 360

aatgagtctg gttttccacc cagcgaggat gaggacgaag agggtgagca gtttgtgtta 420

gattctgtgg aacaacccgg gcgaggatgc aggtcttgtc aatatcaccg gaaaaacaca 480

ggagactccc agattatgtg ttctctgtgt tatatgaaga tgacctgtat gtttatttac 540

agtaagttta tcatcggtgg gcaggtgggc tatagtgtgg gtggtggtct ttggggggtt 600

ttttaatata tgtcaggggt tatgctgaag acttttttat tgtgattttt aaaggtccag 660

tgtctgagcc cgagcaagaa cctgaaccgg agcctgagcc ttctcgcccc aggagaaagc 720

ctgtaatctt aactagaccc agcgcaccgg tagcgagagg cctcagcagc gcggagacca 780

ccgactccgg tgcttcctca tcacccccgg agattcaccc cctggtgccc ctatgtcccg 840

ttaagcccgt tgccgtgaga gtcagtgggc ggcggtctgc tgtggagtgc attgaggact 900

tgctttttga ttcacaggaa cctttggact tgagcttgaa acgccccagg cattaaacct 960

ggtcacctgg actgaatgag ttgacgccta tgtttgcttt tgaatgactt aatgtgtata 1020

gataataaag agtgagataa tgttttaatt gcatggtgtg tttaacttgg gcggagtctg 1080

ctgggtatat aagcttccct gggctaaact tggttacact tgacctcatg gaggcctggg 1140

agtgtttgga gaactttgcc ggagttcgtg ccttgctgga cgagagctct aacaatacct 1200

cttggtggtg gaggtatttg tggggctctc cccagggcaa gttagtttgt agaatcaagg 1260

aggattacaa gtgggaattt gaagagcttt tgaaatcctg tggtgagcta ttggattctt 1320

tgaatctagg ccaccaggct ctcttccagg agaaggtcat caggactttg gatttttcca 1380

caccggggcg cattgcagcc gcggttgctt ttctagcttt tttgaaggat agatggagcg 1440

aagagaccca cttgagttcg ggctacgtcc tggattttct ggccatgcaa ctgtggagag 1500

catggatcag acacaagaac aggctgcaac tgttgtcttc cgtccgcccg ttgctgattc 1560

cggcggagga gcaacaggcc gggtcagagg accgggcccg tcgggatccg gaggagaggg 1620

caccgaggcc gggcgagagg agcgcgctga acctgggaac cgggctgagc ggccatccac 1680

atcgggagtg aatgtcgggc aggtggtgga tctttttcca gaactgcggc ggattttgac 1740

tattagggag gatgggcaat ttgttaaggg tcttaagagg gagagggggg cttctgagca 1800

taacgaggag gccagtaatt tagcttttag cttgatgacc agacaccgtc cagagtgcat 1860

cacttttcag cagattaagg acaattgtgc caatgagttg gatctgttgg gtcagaagta 1920

tagcatagag cagctgacca cttactggct gcagccgggt gatgatctgg aggaagctat 1980

tagggtgtat gctaaggtgg ccctgcggcc cgattgcaag tacaagctca aggggctggt 2040

gaatatcagg aattgttgct acatttctgg caacggggcg gaggtggaga tagagaccga 2100

agacagggtg gctttcagat gcagcatgat gaatatgtgg ccgggggtgc tgggcatgga 2160

cggggtggtg attatgaatg tgaggttcac ggggcccaac tttaacggca cggtgttttt 2220

ggggaacacc aacctggtcc tgcacggggt gagcttctat gggtttaaca acacctgtgt 2280

ggaggcctgg accgatgtga aggtccgcgg ttgcgccttt tatggatgtt ggaaggccat 2340

agtgagccgc cctaagagca ggagttccat taagaaatgc ttgtttgaga ggtgcacctt 2400

ggggatcctg gccgagggca actgcagggt gcgccacaat gtggcctccg agtgcggttg 2460

cttcatgcta gtcaagagcg tggcggtaat caagcataat atggtgtgcg gcaacagcga 2520

ggacaaggcc tcacagatgc tgacctgcac ggatggcaac tgccacttgc tgaagaccat 2580

ccatgtaacc agccacagcc ggaaggcctg gcccgtgttc gagcacaact tgctgacccg 2640

ctgctccttg catctgggca acag 2664

<210> 4

<211> 3509

<212> DNA

<213> Pan troglodytes

<400> 4

atgaacacct cagcctccaa taactctacc ctttcttcgg aatcaggtga cttctctgaa 60

atcgggcttg gtgtgctgct tactctgttg atttttttcc ttatcatact cagccttctg 120

tgcctcaggc tcgccgcctg ctgcgcacac atctatatct actgctggtt gctcaagtgc 180

aggggtcgcc acccaagatg aacaggtaca tggtcctatc gatcctaggc ctgctggccc 240

tggcggcctg cagcgccgcc aaaaaagaga ttacctttga ggagcccgct tgcaatgtaa 300

ctttcaagcc cgagggtgac caatgcacca ccctcgtcaa atgcgttacc aatcatgaga 360

ggctgcgcat cgactacaaa aacaaaactg gccagtttgc ggtctatagt gtgtttacgc 420

ccggagaccc ctctaactac tctgtcaccg tcttccaggg cggacagtct aagatattca 480

attacacttt ccctttttat gagttatgcg atgcggtcat gtacatgtca aaacagtaca 540

acctgtggcc tccctctccc caggcgtgtg tggaaaatac tgggtcttac tgctgtatgg 600

ctttggcaat cactacgctc gctctaatct gcacggtgct atacataaaa ttcaggcaga 660

ggcgaatctt tatcgatgaa aagaaaatgc cttgatcgct aacaccggct ttctatctgc 720

agaatgaatg caatcacctc cctactaatc accaccaccc tccttgcgat tgcccatggg 780

ttgacacgaa tcgaagtgcc agtggggtcc aatgtcacca tggtgggccc cgccggcaat 840

tccaccctca tgtgggaaaa atttgtccgc aatcaatggg ttcatttctg ctctaaccga 900

atcagtatca agcccagagc catctgcgat gggcaaaatc taactctgat caatgtgcaa 960

atgatggatg ctgggtacta ttacgggcag cggggagaaa tcattaatta ctggcgaccc 1020

cacaaggact acatgctgca tgtagtcgag gcacttccca ctaccacccc cactaccacc 1080

tctcccacca ccactaccac cactactact actactacta ccactaccgc tgcccgccat 1140

acccgcaaaa gcaccatgat tagcacaaag ccccctcgtg ctcactccca cgccggcggg 1200

cccatcggtg cgacctcaga aaccaccgag ctttgcttct gccaatgcac taacgccagc 1260

gctcatgaac tgttcgacct ggagaatgag gatgcccagc agagctccgc ttgcctgacc 1320

caggaggctg tggagcccgt tgccctgaag cagatcggtg attcaataat tgactcttct 1380

tcttttgcca ctcccgaata ccctcccgat tctactttcc acatcacggg taccaaagac 1440

cctaacctct ctttctacct gatgctgctg ctctgtatct ctgtggtctc ttccgcgctg 1500

atgttactgg ggatgttctg ctgcctgatc tgccgcagaa agagaaaagc tcgctctcag 1560

ggccaaccac tgatgccctt cccctacccc ccggattttg cagataacaa gatatgagct 1620

cgctgctgac actaaccgct ttactagcct gcgctctaac ccttgtcgct tgcgactcga 1680

gattccacaa tgtcacagct gtggcaggag aaaatgttac tttcaactcc acggccgata 1740

cccagtggtc gtggagtggc tcaggtagct acttaactat ctgcaatagc tccacttccc 1800

ccagcatatc cccaaccaag taccaatgca atgccagcct gttcaccctc atcaacgctt 1860

ccaccctgga caatggactc tatgtaggct atgtaccctt tggtgggcaa ggaaagaccc 1920

acgcttacaa cctggaagtt cgccagccca gaaccactac ccaagcttct cccaccacca 1980

ccaccaccac caccaccacc atcaccagca gcagcagcag ccacagcagc agcagcagat 2040

tattgacttt ggttttggcc agctcatctg ccgctaccca ggccatctac agctctgtgc 2100

ccgaaaccac tcagatccac cgcccagaaa cgaccaccgc caccacccta cacacctcca 2160

gcgatcagat gccgaccaac atcaccccct tggctcttca aatgggactt acaagcccca 2220

ctccaaaacc agtggatgcg gccgaggtct ccgccctcgt caatgactgg gcggggctgg 2280

gaatgtggtg gttcgccata ggcatgatgg cgctctgcct gcttctgctc tggctcatct 2340

gctgcctcca ccgcaggcga gccagacccc ccatctatag acccatcatt gtcctgaacc 2400

ccgataatga tgggatccat agattggatg gcctgaaaaa cctacttttt tcttttacag 2460

tatgataaat tgagacatgc ctcgcatttt cttgtacatg ttccttctcc caccttttct 2520

ggggtgttct acgctggccg ctgtgtctca cctggaggta gactgcctct cacccttcac 2580

tgtctacctg ctttacggat tggtcaccct cactctcatc tgcagcctaa tcacagtaat 2640

catcgccttc atccagtgca ttgattacat ctgtgtgcgc ctcgcatact tcagacacca 2700

cccgcagtac cgagacagga acattgccca acttctaaga ctgctctaat catgcataag 2760

actgtgatct gccttctgat cctctgcatc ctgcccaccc tcacctcctg ccagtacacc 2820

acaaaatctc cgcgcaaaag acatgcctcc tgccgcttca cccaactgtg gaatataccc 2880

aaatgctaca acgaaaagag cgagctctcc gaagcttggc tgtatggggt catctgtgtc 2940

ttagttttct gcagcactgt ctttgccctc atgatctacc cctactttga tttgggatgg 3000

aacgcgatcg atgccatgaa ttaccccacc tttcccgcac ccgagataat tccactgcga 3060

caagttgtac ccgttgtcgt taatcaacgc cccccatccc ctacgcccac tgaaatcagc 3120

tactttaacc taacaggcgg agatgactga cgccctagat ctagaaatgg acggcatcag 3180

taccgagcag cgtctcctag agaggcgcag gcaggcggct gagcaagagc gcctcaatca 3240

ggagctccga gatctcgtta acctgcacca gtgcaaaaga ggcatctttt gtctggtaaa 3300

gcaggccaaa gtcacctacg agaagaccgg caacagccac cgcctcagtt acaaattgcc 3360

cacccagcgc cagaagctgg tgctcatggt gggtgagaat cccatcaccg tcacccagca 3420

ctcggtagag accgaggggt gtctgcactc tccctgtcgg ggtccagaag acctctgcac 3480

cctggtaaag accctgtgcg gtctcagag 3509

<210> 5

<211> 2716

<212> DNA

<213> Pan troglodytes

<400> 5

agatcagaca gtgataatta accaccacca ccaccatacc ttttgattca ggaaatcatg 60

atcatcacag gatcctagtc ttcaggccgc cccctccctc ccaagacaca gaatacacag 120

tcctctcccc ccgactggct ttaaataaca ccatctggtt ggtcacagac atgttcttag 180

gggttatatt ccacacggtc tcctgccgcg ccaggcgctc gtcggtgatg ttgataaact 240

ctcccggcag ctcgctcaag ttcacgtcgc tgtccagcgg ctgaacctcc ggctgacgcg 300

ataactgtgc gaccggctgc tggacaaacg gaggccgcgc ctacaagggg gtagagtcat 360

aatcctcggt caggataggg cggtgatgca gcagcagcga gcgaaacatc tgctgccgcc 420

gccgctccgt ccggcaggaa aacaacaagc cggtggtctc ctccgcgata atccgcaccg 480

cccgcagcat cagcttcctc gttctccgcg cgcagcacct caccctgatc tcgctcaagt 540

cggcgcagta ggtacagcac agcaccacga tgttattcat gatcccacag tgcagggcgc 600

tgtatccaaa gctcatgccg ggaaccaccg cccccacgtg gccatcgtac cacaagcgca 660

cgtaaattaa gtgtcgaccc ctcatgaacg tgctggacac aaacattact tccttgggca 720

tgttgtaatt caccacctcc cggtaccaga taaacctctg gttaaacagg gcaccttcca 780

ccaccatcct gaaccaagag gccagaacct gcccaccggc tatgcactgc agggaacccg 840

ggttggaaca atgacaatgc agactccaag gctcgtaacc gtggatcatc cggctgctga 900

aggcatcgat gttggcacaa cacagacaca cgtgcatgca ctttctcatg attagcagct 960

cttccctcgt caggatcata tcccaaggaa taacccattc ttgaatcaac gtaaaaccca 1020

cacagcaggg aaggcctcgc acataactca cgttgtgcat ggtcagcgtg ttgcattctg 1080

gaaacagcgg atgatcctcc agtatcgagg cgcgggtctc cttctcacag ggaggtaaag 1140

ggtccctgct gtacggactg cgccgggacg accgagatcg tgttgagcgt agtgtcatgg 1200

aaaagggaac gccggacgtg gtcatacttc ttgaagcaga accaggttcg cgcgtggcag 1260

gcctccttgc gtctgcggtc tcgccgtcta gctcgctccg tgtgatagtt gtagtacagc 1320

cactcccgca gagcgtcgag gcgcaccctg gcttccggat ctatgtagac tccgtcttgc 1380

accgcggccc tgataatatc caccaccgta gaataagcaa cacccagcca agcaatacac 1440

tcgctctgcg agcggcagac aggaggagcg ggcagagatg ggagaaccat gataaaaaac 1500

tttttttaaa gaatattttc caattcttcg aaagtaagat ctatcaagtg gcagcgctcc 1560

cctccactgg cgcggtcaaa ctctacggcc aaagcacaga caacggcatt tctaagatgt 1620

tccttaatgg cgtccaaaag acacaccgct ctcaagttgc agtaaactat gaatgaaaac 1680

ccatccggct gattttccaa tatagacgcg ccggcggcgt ccaccaaacc cagataattt 1740

tcttctctcc agcggtttag aatctgtcta agcaaatccc ttatatcaag tccggccatg 1800

ccaaaaatct gctcaagagc gccctccacc ttcatgacca agcagcgcat catgattgca 1860

aaaattcagg ttcttcagag acctgtataa gattcaaaat gggaacatta acaaaaattc 1920

ctctgtcgcg cagatccctt cgcagggcaa gctgaacata atcagacagg tctgaacgga 1980

ccagtgaggc caaatcccca ccaggaacca gatccagaga ccctatactg attatgacgc 2040

gcatactcgg ggctatgctg accagcgtag cgccgatgta ggcgtgctgc atgggcggcg 2100

agataaaatg caaagtgctg gttaaaaaat caggcaaagc ctcgcgcaaa aaagctaaca 2160

catcataatc atgctcatgc aggtagttgc aggtaagctc aggaaccaaa acggaataac 2220

acacgatttt cctctcaaac atgacttcgc ggatactgcg taaaacaaaa attataaata 2280

aaaaattaat taacttaaac attggaagcc tgtctcacaa caggaaaaac cactttaatc 2340

aacataagac gggccacggg catgccggca tagccgtaaa aaaattggtc cccgtgatta 2400

acaagtacca cagacagctc cccggtcatg tcgggggtca tcatgtgaga ctctgtatac 2460

acgtctggat tgtgaacatc agacaaacaa agaaatcgag ccacgtagcc cggaggtata 2520

atcacccgca ggcggaggta cagcaaaacg acccccatag gaggaatcac aaaattagta 2580

ggagaaaaaa atacataaac accagaaaaa ccctgttgct gaggcaaaat agcgccctcc 2640

cgatccaaaa caacataaag cgcttccaca ggagcagcca taacaaagac ccgagtctta 2700

ccagtaaaag aaaaaa 2716

<210> 6

<211> 496

<212> DNA

<213> Artificial sequence

<220>

<223> sequence of cleavage site inserted in deleted E1 region

<400> 6

gcgatcgccg atatcatttc cccgaaaagt gccacctgac cgtaactata acggtcctaa 60

ggtagcgaaa gctcagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 120

atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 180

gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 240

tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgaa 300

ttcatctatg tcgggtgcgg agaaagaggt aatgaaatgg cattatgggt attatgggtc 360

tgcattaatg aatcggccag atatcgacat atgctggcca ccgtacatgt ggcttcccat 420

gctcgcaagc cctggcccga gttcgagcac aatgtcatga ccaggtgcaa tatgcatctg 480

gggtcccggc gatcgc 496

<210> 7

<211> 40

<212> DNA

<213> Artificial sequence

<220>

<223> sequence of cleavage site inserted in deleted E3 region

<400> 7

ggttaacccc ttaattaagg ggattccata tggtacgtac 40

<210> 8

<211> 946

<212> DNA

<213> Pan troglodytes

<220>

<223> ChAd 68E 4 ORF6 sequence inserted in deleted E4 region

<400> 8

aactagtgga gaagtactcg cctattatct ggaagaagca gaagagcggc ggtgggaatc 60

atagtccgcg aacgggatcg gccggtggtg tcgcatcagg ccccgcagca gtcgctgccg 120

ccgccgctcc gtcaagctgc tgctcagggg gtccgggtcc agggactccc tcagcatgat 180

gcccacggcc ctcagcatca gtcgtctggt gcggcgggcg cagcagcgca tgcggatctc 240

gctcaggtcg ctgcagtacg tgcaacacag aaccaccagg ttgttcaaca gtccatagtt 300

caacacgctc cagccgaaac tcatcgcggg aaggatgcta cccacgtggc cgtcgtacca 360

gatcctcagg taaatcaagt ggtgccccct ccagaacacg ctgcccacgt acatgatctc 420

cttgggcatg tggcggttca ccacctcccg gtaccacatc accctctggt tgaacatgca 480

gccccggatg atcctgcgga accacagggc cagcaccgcc ccgcccgcca tgcagcgaag 540

agaccccggg tcccggcaat ggcaatggag gacccaccgc tcgtacccgt ggatcatctg 600

ggagctgaac aagtctatgt tggcacagca caggcatatg ctcatgcatc tcttcagcac 660

tctcaactcc tcgggggtca aaaccatatc ccagggcacg gggaactctt gcaggacagc 720

gaaccccgca gaacagggca atcctcgcac agaacttaca ttgtgcatgg acagggtatc 780

gcaatcaggc agcaccgggt gatcctccac cagagaagcg cgggtctcgg tctcctcaca 840

gcgtggtaag ggggccggcc gatacgggtg atggcgggac gcggctgatc gtgttcgcga 900

ccgtgtcatg atgcagttgc tttcggacat atttcctgaa gtctta 946

<210> 9

<211> 2259

<212> DNA

<213> Artificial sequence

<220>

<223> modified prokaryotic replication plasmid empty vector sequence

<400> 9

aaatggcgcg ccgggcccgg gccgcatagt taagccagcc ccgacacccg ccaacacccg 60

ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg 120

tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa 180

agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga 240

cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa 300

tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt 360

gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg 420

cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag 480

atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg 540

agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg 600

gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt 660

ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga 720

cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac 780

ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc 840

atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc 900

gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac 960

tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag 1020

gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg 1080

gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta 1140

tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg 1200

ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata 1260

tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt 1320

ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc 1380

ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct 1440

tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa 1500

ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag 1560

tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc 1620

tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg 1680

actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca 1740

cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagcttt 1800

gagatagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg 1860

tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc 1920

ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc 1980

ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc 2040

cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg 2100

cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga 2160

gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc 2220

attaatgcag ctggcacgac aggtttcccg actggattt 2259

<210> 10

<211> 2466

<212> DNA

<213> Artificial sequence

<220>

<223> EGFP-containing exogenous reporter gene encoding expression reading frame sequence inserted between two AsiSI of deleted E1 region

<400> 10

cgatatcatt tccccgaaaa gtgccacctg accgtaacta taacggtcct aaggtagcga 60

aagctcagat ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca 120

tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc 180

aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg 240

gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 300

ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 360

ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 420

ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 480

cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 540

atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 600

cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 660

attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 720

cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 780

caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 840

cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcactaga 900

agctttattg cggtagttta tcacagttaa attgctaacg cagtcagtgc ttctgacaca 960

acagtctcga acttaagctg cagaagttgg tcgtgaggca ctgggcaggt aagtatcaag 1020

gttacaagac aggtttaagg agaccaatag aaactgggct tgtcgagaca gagaagactc 1080

ttgcgtttct gataggcacc tattggtctt actgacatcc actttgcctt tctctccaca 1140

ggtgtccact cccagttcaa ttacagctct taaggctaga gtactctagc ccgggatcca 1200

ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 1260

gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 1320

gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg 1380

ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc 1440

gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 1500

cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 1560

ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 1620

atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac 1680

aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc 1740

gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 1800

cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc 1860

gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 1920

ctgtacaagt aaagcggccg ccactgtgct ggatgatccg agctcggtac ctctagagtc 1980

gacccgggcg gccaaaccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg 2040

ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt 2100

cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg 2160

gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg 2220

atgcggtggg ctctatggct tctgaggcgg aaagaaccag cagatctgca gatctgaatt 2280

catctatgtc gggtgcggag aaagaggtaa tgaaatggca ttatgggtat tatgggtctg 2340

cattaatgaa tcggccagat atcgacatat gctggccacc gtacatgtgg cttcccatgc 2400

tcgcaagccc tggcccgagt tcgagcacaa tgtcatgacc aggtgcaata tgcatctggg 2460

gtcccg 2466

<210> 11

<211> 32601

<212> DNA

<213> Artificial sequence

<220>

<223> recombinant chimpanzee ChAd3 type adenovirus empty vector plasmid sequence

<400> 11

aaatcatcat caataatata ccttattttg gattgaagcc aatatgataa tgagatgggc 60

ggcgcgaggc ggggcgcggg gcgggaggcg ggtttggggg cgggccggcg ggcggggcgg 120

tgtggcggaa gtggactttg taagtgtggc ggatgtgact tgctagtgcc gggcgcggta 180

aaagtgacgt tttccgtgcg cgacaacgcc cccgggaagt gacatttttc ccgcggtttt 240

taccggatgt tgtagtgaat ttgggcgtaa ccaagtaaga tttggccatt ttcgcgggaa 300

aactgaaacg gggaagtgaa atctgattaa ttttgcgtta gtcataccgc gtaatatttg 360

tctagggccg agggactttg gccgattacg tggaggactc gcccaggtgt tttttgaggt 420

gaatttccgc gttccgggtc aaagtctccg ttttattatt atagtcagct gacgcggagt 480

gtatttatac cctctgatct cgtcaagagg ccactcttga gtgccagcga gtagagtttt 540

ctcctctgcc gctctccgct ccgctccgct cggctctgac accggggaaa aagcgatcgc 600

cgatatcatt tccccgaaaa gtgccacctg accgtaacta taacggtcct aaggtagcga 660

aagctcagat ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca 720

tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc 780

aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg 840

gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg aattcatcta 900

tgtcgggtgc ggagaaagag gtaatgaaat ggcattatgg gtattatggg tctgcattaa 960

tgaatcggcc agatatcgac atatgctggc caccgtacat gtggcttccc atgctcgcaa 1020

gccctggccc gagttcgagc acaatgtcat gaccaggtgc aatatgcatc tggggtcccg 1080

gcgatcgcgc ggggggtgtt cctgccctat caatgcaact ttagtcacac caagatcttg 1140

ctagagcccg agagcatgtc caaggtgaac ttgaacgggg tgtttgacat gaccatgaag 1200

atctggaagg tgctgaggta cgacgagacc aggtcccggt gcagaccctg cgagtgcggg 1260

ggcaagcata tgaggaacca gcccgtgatg ctggatgtga ccgaggagct gaggacagac 1320

cacttggttc tggcctgcac cagggccgag tttggttcta gcgatgaaga cacagattga 1380

ggtgggtgag tgggcgtggc ctggggtggt catgaaaata tataagttgg gggtcttagg 1440

gtctctttat ttgtgttgca gagaccgccg gagccatgag cgggagcagc agcagcagca 1500

gtagcagcag cgccttggat ggcagcatcg tgagccctta tttgacgacg cggatgcccc 1560

actgggccgg ggtgcgtcag aatgtgatgg gctccagcat cgacggccga cccgtcctgc 1620

ccgcaaattc cgccacgctg acctatgcga ccgtcgcggg gacgccgttg gacgccaccg 1680

ccgccgccgc cgccaccgca gccgcctcgg ccgtgcgcag cctggccacg gactttgcat 1740

tcctgggacc actggcgaca ggggctactt ctcgggccgc tgctgccgcc gttcgcgatg 1800

acaagctgac cgccctgctg gcgcagttgg atgcgcttac tcgggaactg ggtgaccttt 1860

ctcagcaggt catggccctg cgccagcagg tctcctccct gcaagctggc gggaatgctt 1920

ctcccacaaa tgccgtttaa gataaataaa accagactct gtttggatta aagaaaagta 1980

gcaagtgcat tgctctcttt atttcataat tttccgcgcg cgataggccc tagaccagcg 2040

ttctcggtcg ttgagggtgc ggtgtatctt ctccaggacg tggtagaggt ggctctggac 2100

gttgagatac atgggcatga gcccgtcccg ggggtggagg tagcaccact gcagagcttc 2160

atgctccggg gtggtgttgt agatgatcca gtcgtagcag gagcgctggg catggtgcct 2220

aaaaatgtcc ttcagcagca ggccgatggc cagggggagg cccttggtgt aagtgtttac 2280

aaaacggtta agttgggaag ggtgcattcg gggagagatg atgtgcatct tggactgtat 2340

ttttagattg gcgatgtttc cgcccagatc ccttctggga ttcatgttgt gcaggaccac 2400

cagtacagtg tatccggtgc acttggggaa tttgtcatgc agcttagagg gaaaagcgtg 2460

gaagaacttg gagacgccct tgtggcctcc cagattttcc atgcattcgt ccatgatgat 2520

ggcaatgggc ccgcgggagg cagcttgggc aaagatattt ctggggtcgc tgacgtcgta 2580

gttgtgttcc agggtgaggt cgtcataggc catttttaca aagcgcgggc ggagggtgcc 2640

cgactggggg atgatggtcc cctctggccc tggggcgtag ttgccctcgc agatctgcat 2700

ttcccaggcc ttaatctcgg aggggggaat catatccacc tgcggggcga tgaagaaaac 2760

ggtttccgga gccggggaga ttaactggga tgagagcagg tttctaagca gctgtgattt 2820

tccacaaccg gtgggcccat aaataacacc tataaccggt tgcagctggt agtttagaga 2880

gctgcagctg ccgtcgtccc ggaggagggg ggccacctcg ttgagcatgt ccctgacgcg 2940

catgttctcc ccgaccagat ccgccagaag gcgctcgccg cccagggaca gcagctcttg 3000

caaggaagca aagtttttca gcggcttgag gccgtccgcc gtgggcatgt ttttcagggt 3060

ctggctcagc agctccaggc ggtcccagag ctcggtgacg tgctctacgg catctctatc 3120

cagcatatct cctcgtttcg cgggttgggg cgactttcgc tgtagggcac caagcggtgg 3180

tcgtccagcg gggccaaagt catgtccttc catgggcgca gggtcctcgt cagggtggtc 3240

tgggtcacgg tgaaggggtg cgctccgggc tgagcgcttg ccaaggtgcg cttgaggctg 3300

gttctgctgg tgctgaagcg ctgccggtct tcgccctgcg cgtcggccag gtagcatttg 3360

accatggtgt catagtccag cccctccgcg gcgtgtccct tggcgcgcag cttgcccttg 3420

gaggtggcgc cgcacgaggg gcagagcagg ctcttgagcg cgtagagctt gggggcgagg 3480

aagaccgatt cgggggagta ggcgtccgcg ccgcagaccc cgcacacggt ctcgcactcc 3540

accagccagg tgagctcggg gcgcgccggg tcaaaaacca ggtttccccc atgctttttg 3600

atgcgtttct tacctcgggt ctccatgagg tggtgtcccc gctcggtgac gaagaggctg 3660

tccgtgtctc cgtagaccga cttgaggggt cttttctcca ggggggtccc tcggtcttcc 3720

tcgtagagga actcggacca ctctgagacg aaggcccgcg tccaggccag gacgaaggag 3780

gctatgtggg aggggtagcg gtcgttgtcc actagggggt ccaccttctc caaggtgtga 3840

agacacatgt cgccttcctc ggcgtccagg aaggtgattg gcttgtaggt gtaggccacg 3900

tgaccggggg ttcctgacgg gggggtataa aagggggtgg gggcgcgctc gtcgtcactc 3960

tcttccgcat cgctgtctgc gagggccagc tgctggggtg agtattccct ctcgaaggcg 4020

ggcatgacct ccgcgctgag gttgtcagtt tccaaaaacg aggaggattt gatgttcacc 4080

tgtcccgagg tgataccttt gagggtaccc gcgtccatct ggtcagaaaa cacgatcttt 4140

ttattgtcca gcttggtggc gaacgacccg tagagggcgt tggagagcag cttggcgatg 4200

gagcgcaggg tctggttctt gtccctgtcg gcgcgctcct tggccgcgat gttgagctgc 4260

acgtactcgc gcgcgacgca gcgccactcg gggaagacgg tggtgcgctc gtcgggcacc 4320

aggcgcacgc gccagccgcg gttgtgcagg gtgaccaggt ccacgctggt ggcgacctcg 4380

ccgcgcaggc gctcgttggt ccagcagaga cggccgccct tgcgcgagca gaaggggggc 4440

agggggtcga gctgggtctc gtccgggggg tccgcgtcca cggtgaaaac cccggggcgc 4500

aggcgcgcgt cgaagtagtc tatcttgcaa ccttgcatgt ccagcgcctg ctgccagtcg 4560

cgggcggcga gcgcgcgctc gtaggggttg agcggcgggc cccagggcat ggggtgggtg 4620

agtgcggagg cgtacatgcc gcagatgtca tagacgtaga ggggctcccg caggaccccg 4680

atgtaggtgg ggtagcagcg gccgccgcgg atgctggcgc gcacgtagtc atacagctcg 4740

tgcgaggggg cgaggaggtc ggggcccagg ttggtgcggg cggggcgctc cgcgcggaag 4800

acgatctgcc tgaagatggc atgcgagttg gaagagatgg tggggcgctg gaagacgttg 4860

aagctggcgt cctgcaggcc gacggcgtcg cgcacgaagg aggcgtagga gtcgcgcagc 4920

ttgtgtacca gctcggcggt gacctgcacg tcgagcgcgc agtagtcgag ggtctcgcgg 4980

atgatgtcat atttagcctg ccccttcttt ttccacagct cgcggttgag gacaaactct 5040

tcgcggtctt tccagtactc ttggatcggg aaaccgtccg gttccgaacg gtaagagcct 5100

agcatgtaga actggttgac ggcctggtag gcgcagcagc ccttctccac ggggagggcg 5160

taggcctgcg cggccttgcg gagcgaggtg tgggtcaggg cgaaggtgtc cctgaccatg 5220

actttgaggt actggtgctt gaagtcggag tcgtcgcagc cgccccgctc ccagagcgag 5280

aagtcggtgc gcttcttgga gcgggggttg ggcagagcga aggtgacatc gttgaagagg 5340

attttgcccg cgcggggcat gaagttgcgg gtgatgcgga agggccccgg cacttcagag 5400

cggttgttga tgacctgggc ggcgagcacg atctcgtcga agccgttgat gttgtggccc 5460

acgatgtaga gttccaggaa gcggggccgg ccctttacgg tgggcagctt ctttagctct 5520

tcgtaggtga gctcctcggg cgaggcgagg ccgtgctcgg ccagggccca gtccgcgagg 5580

tgcgggttgt ctctgaggaa ggactcccag aggtcgcggg ccaggagggt ctgcaggcgg 5640

tccctgaagg tcctgaactg gcggcccacg gccatttttt cgggggtgat gcagtagaag 5700

gtgagggggt cttgctgcca gcggtcccag tcgagctgca gggcgaggtc gcgcgcggcg 5760

gtgaccaggc gctcgtcgcc cccgaatttc atgaccagca tgaagggcac gagctgcttt 5820

ccgaaggccc ccatccaagt gtaggtctct acatcgtagg tgacaaagag gcgctccgtg 5880

cgaggatgcg agccgatcgg gaagaactgg atctcccgcc accagttgga ggagtggctg 5940

ttgatgtggt ggaagtagaa gtcccgtcgc cgggccgaac actcgtgctg gcttttgtaa 6000

aagcgagcgc agtactggca gcgctgcacg ggctgtacct cctgcacgag atgcaccttt 6060

cgcccgcgca cgaggaagcc gaggggaaat ctgagccccc cgcctggctc gcggcatggc 6120

tggtgctctt ctactttgga tgcgtgtccg tctccgtctg gctcctcgag gggtgttacg 6180

gtggagcgga ccaccacgcc gcgcgagccg caggtccaga tatcggcgcg cggcggtcgg 6240

agtttgatga cgacatcgcg cagctgggag ctgtccatgg tctggagctc ccgcggcggc 6300

ggcaggtcag ccgggagttc ttgcaggttc acctcgcaga gtcgggccag ggcgcggggc 6360

aggtctaggt ggtacctgat ctctaggggc gtgttggtgg cggcgtcgat ggcttgcagg 6420

agcccgcatc cccggggggc gacgacggtg ccccgcgggg tggtggtggt ggtggtggtg 6480

gtggtggtgg cggtgcagct cagaagcggt gccgcgggcg ggcccccgga ggtagggggg 6540

gctccggtcc cgccggcagg ggcggcagcg gcacgtcggc gtggagcgcg ggcaggagtt 6600

ggtgctgtgc ccggaggttg ctggcgaagg cgacgacgcg gcggttgatc tcctggatct 6660

ggcgcctctg cgtgaagacg acgggcccgg tgagcttgaa cctgaaagag agttcgacag 6720

aatcaatctc ggtgtcattg accgcggcct ggcgcaggat ctcctgcacg tctcccgagt 6780

tgtcttggta ggcgatctcg gccatgaact gctcgatctc ttcctcctgg aggtctccgc 6840

gtccggcgcg ttccacggtg gccgccaggt cgttggagat gcgccccatg agctgcgaga 6900

aggcgttgag tccgccctcg ttccagactc ggctgtagac cacgcccccc tggtcatcgc 6960

gggcgcgcat gaccacctgc gcgaggttga gctccacgtg ccgcgcgaag acggcgtagt 7020

tgcgcagacg ctggaagagg tagttgaggg tggtggcggt gtgctcggcc acgaagaagt 7080

tcatgaccca gcggcgcaac gtggattcgt tgatgtcccc caaggcctcc agccgttcca 7140

tggcctcgta gaagtccacg gcgaagttga aaaactggga gttgcgcgcc gacacggtca 7200

actcctcctc cagaagacgg atgagctcgg cgacggtgtc gcgcacctcg cgctcgaagg 7260

ctatggggat ctcttcctcc gctagcatca ccacctcctc ctcttcctcc tcttctggca 7320

cttccatgat ggcttcctcc tcttcggggg gcggcggcgg cggcggtggg ggagggggcg 7380

ctctgcgccg gcggcggcgc accgggaggc ggtccacgaa gcgcgcgatc atctccccgc 7440

ggcggcggcg catggtctcg gtgacggcgc ggccgttctc ccgggggcgc agttggaaga 7500

cgccgccgga catctggtgc tggggcgggt ggccgtgagg cagcgaaacg gcgctgacga 7560

tgcatctcaa caattgctgc gtaggtacgc cgccgaggga cctgagggag tccatatcca 7620

ccggatccga aaacctttcg aggaaggcgt ctaaccagtc gcagtcgcaa ggtaggctga 7680

gcaccgtggc gggcggcggg gggtgggggg agtgtctggc ggaggtgctg ctgatgatgt 7740

aattgaagta ggcggacttg acacggcgga tggtcgacag gagcaccatg tccttgggtc 7800

cggcctgctg gatgcggagg cggtcggcta tgccccaggc ttcgttctgg catcggcgca 7860

ggtccttgta gtagtcttgc atgagccttt ccaccggcac ctcttctcct tcctcttctg 7920

cttcttccat gtctgcttcg gccctggggc ggcgccgcgc ccccctgccc cccatgcgcg 7980

tgaccccgaa ccccctgagc ggttggagca gggccaggtc ggcgacgacg cgctcggcca 8040

ggatggcctg ctgcacctgc gtgagggtgg tttggaagtc atccaagtcc acgaagcggt 8100

ggtaggcgcc cgtgttgatg gtgtaggtgc agttggccat gacggaccag ttgacggtct 8160

ggtggcccgg ttgcgacatc tcggtgtacc tgagtcgcga gtaggcgcgg gagtcgaaga 8220

cgtagtcgtt gcaagtccgc accaggtact ggtagcccac caggaagtgc ggcggcggct 8280

ggcggtagag gggccagcgc agggtggcgg gggctccggg ggccaggtct tccagcatga 8340

ggcggtggta ggcgtagatg tacctggaca tccaggtgat acccgcggcg gtggtggagg 8400

cgcgcgggaa gtcgcgcacc cggttccaga tgttgcgcag gggcagaaag tgctccatgg 8460

taggcgtgct ctgtccagtc agacgcgcgc agtcgttgat actctagacc agggaaaacg 8520

aaagccggtc agcgggcact cttccgtggt ctggtgaata gatcgcaagg gtatcatggc 8580

ggagggcctc ggttcgagcc ccgggtccgg gccggacggt ccgccatgat ccacgcggtt 8640

accgcccgcg tgtcgaaccc aggtgtgcga cgtcagacaa cggtggagtg ttccttttgg 8700

cgtttttctg gccgggcgcc ggcgtcgcgt aagagactaa gccgcgaaag cgaaagcagt 8760

aagtggctcg ctccccgtag ccggagggat ccttgctaag ggttgcgttg cggcgaaccc 8820

cggttcgaat cccgtactcg ggccggccgg acccgcggct aaggtgttgg attggcctcc 8880

ccctcgtata aagaccccgc ttgcggattg actccggaca cggggacgag ccccttttat 8940

ttttgctttc cccagatgca tccggtgctg cggcagatgc gccccccgcc ccagcagcag 9000

caacaacacc agcaagagcg gcagcaacag cagcgggagt catgcagggc cccctcaccc 9060

accctcggcg ggccggccac ctcggcgtcc gcggccgtgt ctggcgcctg cggcggcggc 9120

ggggggccgg ctgacgaccc cgaggagccc ccgcggcgca gggccagaca ctacctggac 9180

ctggaggagg gcgagggcct ggcgcggctg ggggcgccgt ctcccgagcg ccacccgcgg 9240

gtgcagctga agcgcgactc gcgcgaggcg tacgtgcctc ggcagaacct gttcagggac 9300

cgcgcgggcg aggagcccga ggagatgcgg gacaggaggt tcagcgcagg gcgggagctg 9360

cggcaggggc tgaaccgcga gcggctgctg cgcgaggagg actttgagcc cgacgcgcgg 9420

acggggatca gccccgcgcg cgcgcacgtg gcggccgccg acctggtgac ggcgtacgag 9480

cagacggtga accaggagat caacttccaa aagagtttca acaaccacgt gcgcacgctg 9540

gtggcgcgcg aggaggtgac catcgggctg atgcacctgt gggactttgt aagcgcgctg 9600

gtgcagaacc ccaacagcaa gcctctgacg gcgcagctgt tcctgatagt gcagcacagc 9660

agggacaacg aggcgtttag ggacgcgctg ctgaacatca ccgagcccga gggtcggtgg 9720

ctgctggacc tgattaacat cctgcagagc atagtggtgc aggagcgcag cctgagcctg 9780

gccgacaagg tggcggccat caactactcg atgctgagcc tgggcaagtt ttacgcgcgc 9840

aagatctacc agacgccgta cgtgcccata gacaaggagg tgaagatcga cggtttttac 9900

atgcgcatgg cgctgaaggt gctcaccctg agcgacgacc tgggcgtgta ccgcaacgag 9960

cgcatccaca aggccgtgag cgtgagccgg cggcgcgagc tgagcgaccg cgagctgatg 10020

cacagcctgc agcgggcgct ggcgggcgcc ggcagcggcg acagggaggc ggagtcctac 10080

ttcgatgcgg gggcggacct gcgctgggcg cccagccggc gggccctgga ggccgcgggg 10140

gtccgcgagg actatgacga ggacggcgag gaggatgagg agtacgagct agaggagggc 10200

gagtacctgg actaaaccgc gggtggtgtt tccggtagat gcaagacccg aacgtggtgg 10260

acccggcgct gcgggcggct ctgcagagcc agccgtccgg ccttaactcc tcagacgact 10320

ggcgacaggt catggaccgc atcatgtcgc tgacggcgcg taacccggac gcgttccggc 10380

agcagccgca ggccaacagg ctctccgcca tcctggaggc ggtggtgcct gcgcgctcga 10440

accccacgca cgagaaggtg ctggccatag tgaacgcgct ggccgagaac agggccatcc 10500

gcccggacga ggccgggctg gtgtacgacg cgctgctgca gcgcgtggcc cgctacaaca 10560

gcggcaacgt gcagaccaac ctggaccggc tggtggggga cgtgcgcgag gcggtggcgc 10620

agcgcgagcg cgcggatcgg cagggcaacc tgggctccat ggtggcgctg aatgccttcc 10680

tgagcacgca gccggccaac gtgccgcggg ggcaggaaga ctacaccaac tttgtgagcg 10740

cgctgcggct gatggtgacc gagacccccc agagcgaggt gtaccagtcg ggcccggact 10800

acttcttcca gaccagcaga cagggcctgc agacggtgaa cctgagccag gctttcaaga 10860

acctgcgggg gctgtggggc gtgaaggcgc ccaccggcga ccgggcgacg gtgtccagcc 10920

tgctgacgcc caactcgcgc ctgctgctgc tgctgatcgc gccgttcacg gacagcggca 10980

gcgtgtcccg ggacacctac ctggggcacc tgctgaccct gtaccgcgag gccatcgggc 11040

aggcgcaggt ggacgagcac accttccagg agatcaccag cgtgagccgc gcgctggggc 11100

aggaggacac gagcagcctg gaggcgactc tgaactacct gctgaccaac cggcggcaga 11160

agattccctc gctgcacagc ctgacctccg aggaggagcg catcttgcgc tacgtgcagc 11220

agagcgtgag cctgaacctg atgcgcgacg gggtgacgcc cagcgtggcg ctggacatga 11280

ccgcgcgcaa catggaaccg ggcatgtacg ccgcgcaccg gccttacatc aaccgcctga 11340

tggactacct gcatcgcgcg gcggccgtga accccgagta ctttaccaac gccatcctga 11400

acccgcactg gctcccgccg cccgggttct acagcggggg cttcgaggtc ccggaggcca 11460

acgatggctt cctgtgggac gacatggacg acagcgtgtt ctccccgcgg ccgcaggcgc 11520

tggcggaagc gtccctgctg cgtcccaaga aggaggagga ggaggcgagt cgccgccgcg 11580

gcagcagcgg cgtggcttct ctgtccgagc tgggggcggc agccgccgcg cgccccgggt 11640

ccctgggcgg cagccccttt ccgagcctgg tggggtctct gcacagcgag cgcaccaccc 11700

gccctcggct gctgggcgag gacgagtacc tgaataactc cctgctgcag ccggtgcggg 11760

agaaaaacct gccccccgcc ttccccaaca acgggataga gagcctggtg gacaagatga 11820

gcagatggaa gacctatgcg caggagcaca gggacgcgcc cgcgctccgg ccgcccacgc 11880

ggcgccagcg ccacgaccgg cagcgggggc tggtgtggga tgacgaggac tccgcggacg 11940

atagcagcgt gctggacctg ggagggagcg gcaacccgtt cgcgcacctg cgcccccgcc 12000

tggggaggat gttttaaaaa aaaaaaaagc aagaagcatg atgcaaaatt aaataaaact 12060

caccaaggcc atggcgaccg agcgttggtt tcttgtgttc ccttcagtat gcggcgcgcg 12120

gcgatgtacc aggagggacc tcctccctct tacgagagcg tggtgggcgc ggcggcggcg 12180

gcgccctctt ctccctttgc gtcgcagctg ctggagccgc cgtacgtgcc tccgcgctac 12240

ctgcggccta cgggggggag aaacagcatc cgttactcgg agctggcgcc cctgttcgac 12300

accacccggg tgtacctggt ggacaacaag tcggcggacg tggcctccct gaactaccag 12360

aacgaccaca gcaatttttt gaccacggtc atccagaaca atgactacag cccgagcgag 12420

gccagcaccc agaccatcaa tctggatgac cggtcgcact ggggcggcga cctgaaaacc 12480

atcctgcaca ccaacatgcc caacgtgaac gagttcatgt tcaccaataa gttcaaggcg 12540

cgggtgatgg tgtcgcgctc gcacaccaag gaagaccggg tggagctgaa gtacgagtgg 12600

gtggagttcg agctgccaga gggcaactac tccgagacca tgaccattga cctgatgaac 12660

aacgcgatcg tggagcacta tctgaaagtg ggcaggcaaa acggggtcct ggagagcgac 12720

atcggggtca agttcgacac caggaacttc cgcctggggc tggaccccgt gaccgggctg 12780

gttatgcccg gggtgtacac caacgaggcc ttccatcccg acatcatcct gctgcccggc 12840

tgcggggtgg acttcactta cagccgcctg agcaacctcc tgggcatccg caagcggcag 12900

cccttccagg agggcttcag gatcacctac gaggacctgg aggggggcaa catccccgcg 12960

ctcctcgatg tggaggccta ccaggatagc ttgaaggaaa atgaggcggg acaggaggat 13020

accacccccg ccgcctccgc cgccgccgag cagggcgagg atgctgctga caccgcggcc 13080

gcggacgggg cagaggccga ccccgctatg gtggtggagg ctcccgagca ggaggaggat 13140

atgaatgaca gtgcggtgcg cggagacacc ttcgtcaccc ggggggagga aaagcaagcg 13200

gaggccgagg ccgcggccga ggaaaagcaa ctggcggcag cagcggcggc ggcggcgttg 13260

gccgcggcgg aggctgagtc tgaggggacc aagcccgcca aggagcccgt gattaagccc 13320

ctgaccgaag atagcaagaa gcgcagttac aacctgctca aggacagcac caacaccgcg 13380

taccgcagct ggtacctggc ctacaactac ggcgacccgt cgacgggggt gcgctcctgg 13440

accctgctgt gcacgccgga cgtgacctgc ggctcggagc aggtgtactg gtcgctgccc 13500

gacatgatgc aagaccccgt gaccttccgc tccacgcggc aggtcagcaa cttcccggtg 13560

gtgggcgccg agctgctgcc cgtgcactcc aagagcttct acaacgacca ggccgtctac 13620

tcccagctca tccgccagtt cacctctctg acccacgtgt tcaatcgctt tcctgagaac 13680

cagattctgg cgcgcccgcc cgcccccacc atcaccaccg tcagtgaaaa cgttcctgct 13740

ctcacagatc acgggacgct accgctgcgc aacagcatcg gaggagtcca gcgagtgacc 13800

gttactgacg ccagacgccg cacctgcccc tacgtttaca aggccttggg catagtctcg 13860

ccgcgcgtcc tttccagccg cactttttga gcaacaccac catcatgtcc atcctgatct 13920

cacccagcaa taactccggc tggggactgc tgcgcgcgcc cagcaagatg ttcggagggg 13980

cgaggaagcg ttccgagcag caccccgtgc gcgtgcgcgg gcacttccgc gccccctggg 14040

gagcgcacaa acgcggccgc gcggggcgca ccaccgtgga cgacgccatc gactcggtgg 14100

tggagcaggc gcgcaactac aggcccgcgg tctctaccgt ggacgcggcc atccagaccg 14160

tggtgcgggg cgcgcggcgg tacgccaagc tgaagagccg ccggaagcgc gtggcccgcc 14220

gccaccgccg ccgacccggg gccgccgcca aacgcgccgc cgcggccctg cttcgccggg 14280

ccaagcgcac gggccgccgc gccgccatga gggccgcgcg ccgcttggcc gccggcatca 14340

ccgccgccac catggccccc cgtacccgaa gacgcgcggc cgccgccgcc gccgccgcca 14400

tcagtgacat ggccagcagg cgccggggca acgtgtactg ggtgcgcgac tcggtgaccg 14460

gcacgcgcgt gcccgtgcgc ttccgccccc cgcggacttg agatgatgtg aaaaaacaac 14520

actgagtctc ctgctgttgt gtgtatccca gcggcggcgg cgcgcgcagc gtcatgtcca 14580

agcgcaaaat caaagaagag atgctccagg tcgtcgcgcc ggagatctat gggcccccga 14640

agaaggaaga gcaggattcg aagccccgca agataaagcg ggtcaaaaag aaaaagaaag 14700

atgatgacga tgccgatggg gaggtggagt tcctgcgcgc cacggcgccc aggcgcccgg 14760

tgcagtggaa gggccggcgc gtaaagcgcg tcctgcgccc cggcaccgcg gtggtcttca 14820

cgcccggcga gcgctccacc cggactttca agcgcgtcta tgacgaggtg tacggcgacg 14880

aagacctgct ggagcaggcc aacgagcgct tcggagagtt tgcttacggg aagcgtcagc 14940

gggcgctggg gaaggaggac ctgctggcgc tgccgctgga ccagggcaac cccaccccca 15000

gtctgaagcc cgtgaccctg cagcaggtgc tgccgagcag cgcaccctcc gaggcgaagc 15060

ggggtctgaa gcgcgagggc ggcgacctgg cgcccaccgt gcagctcatg gtgcccaagc 15120

ggcagaggct ggaggatgtg ctggagaaaa tgaaagtaga ccccggtctg cagccggaca 15180

tcagggtccg tcccatcaag caggtggcgc cgggcctcgg cgtgcagacc gtggacgtgg 15240

tcatccccac cggcaactcc cccgccgcca ccaccactac cgctgcctcc acggacatgg 15300

agacacagac cgatcccgcc gcagccgcag ccgccgccgc agccgcgacc tcctcggcgg 15360

aggtgcagac ggacccctgg ctgccgccgg cgatgtcagc tccccgcgcg cgccgcggac 15420

gcagaaagta cggcgccgcc aacgcgctcc tgcccgagta cgccttgcat ccttccatcg 15480

cgcccacccc cggctaccga ggctatacct accgcccgcg aagagccaag ggttccaccc 15540

gccgtccccg ccgacgcgcc gccgccacca cccgccgccg ccgccgcaga cgccagcccg 15600

cactggctcc agtctccgtg aggagagtgg cgcgcgacgg acacaccctg gtgctgccca 15660

gggcgcgcta ccaccccagc atcgtttaaa agcctgttgt ggttcttgca gatatggccc 15720

tcacttgccg cctccgtttc ccggtgccgg gataccgagg aggaagatcg cgccgcagga 15780

ggggtctggc cggccgcggc ctgagcggag gcagccgccg cgcgcaccgg cggcgacgcg 15840

ccaccagccg acgcatgcgc ggcggggtgc tgcccctgtt aatccccctg atcgccgcgg 15900

cgatcggcgc cgtgcccggg atcgcctccg tggccttgca agcgtcccag aggcattgac 15960

agacttgcaa acttgcaaat atggaaaaaa aaaaaaaacc ccaataaaaa gtctagactc 16020

tcacgctcgc ttggtcctgt gactattttg tagaatggaa gacatcaact ttgcgtcgct 16080

ggccccgcgt cacggctcgc gcccgttcct gggacactgg aacgatatcg gcaccagcaa 16140

catgagcggt ggcgccttca gttggggctc tctgtggagc ggcattaaaa gtatcgggtc 16200

tgccgttaaa aattacggct cccgggcctg gaacagcagc acgggccaga tgttgagaga 16260

caagttgaaa gagcagaact tccagcagaa ggtggtggag ggcctggcct ccggcatcaa 16320

cggggtggtg gacctggcca accaggccgt gcagaataaa atcaacagca gactggaccc 16380

ccggccgccg gtggaggagg tgccgccggc gctggagacg gtgtcccccg atgggcgtgg 16440

cgagaagcgc ccgcggcccg atagggaaga gaccactctg gtcacgcaga ccgatgagcc 16500

gcccccgtat gaggaggccc taaagcaagg tctgcccacc acgcggccca tcgcgcccat 16560

ggccaccggg gtggtgggcc gccacacccc cgccacgctg gacttgcctc cgcccgccga 16620

tgtgccgcag cagcagaagg cggcacagcc gggcccgccc gcgaccgcct cccgttcctc 16680

cgccggtcct ctgcgccgcg cggccagcgg cccccgcggg ggggtcgcga ggcacggcaa 16740

ctggcagagc acgctgaaca gcatcgtggg tctgggggtg cggtccgtga agcgccgccg 16800

atgctactga atagcttagc taacgtgttg tatgtgtgta tgcgccctat gtcgccgcca 16860

gaggagctgc tgagtcgccg ccgttcgcgc gcccaccacc accgccactc cgcccctcaa 16920

gatggcgacc ccatcgatga tgccgcagtg gtcgtacatg cacatctcgg gccaggacgc 16980

ctcggagtac ctgagccccg ggctggtgca gttcgcccgc gccaccgaga gctacttcag 17040

cctgagtaac aagtttagga accccacggt ggcgcccacg cacgatgtga ccaccgaccg 17100

gtctcagcgc ctgacgctgc ggttcattcc cgtggaccgc gaggacaccg cgtactcgta 17160

caaggcgcgg ttcaccctgg ccgtgggcga caaccgcgtg ctggacatgg cctccaccta 17220

ctttgacatc cgcggggtgc tggaccgggg tcccactttc aagccctact ctggcaccgc 17280

ctacaactcc ctggccccca agggcgctcc caactcctgc gagtgggagc aagaggaaac 17340

tcaggcagtt gaagaagcag cagaagagga agaagaagat gctgacggtc aagctgagga 17400

agagcaagca gctaccaaaa agactcatgt atatgctcag gctccccttt ctggcgaaaa 17460

aattagtaaa gatggtctgc aaataggaac ggacgctaca gctacagaac aaaaacctat 17520

ttatgcagac cctacattcc agcccgaacc ccaaatcggg gagtcccagt ggaatgaggc 17580

agatgctaca gtcgccggcg gtagagtgct aaagaaatct actcccatga aaccatgcta 17640

tggttcctat gcaagaccca caaatgctaa tggaggtcag ggtgtactaa cggcaaatgc 17700

ccagggacag ctagaatctc aggttgaaat gcaattcttt tcaacttctg aaaacgcccg 17760

taacgaggct aacaacattc agcccaaatt ggtgctgtat agtgaggatg tgcacatgga 17820

gaccccggat acgcaccttt cttacaagcc cgcaaaaagc gatgacaatt caaaaatcat 17880

gctgggtcag cagtccatgc ccaacagacc taattacatc ggcttcagag acaactttat 17940

cggcctcatg tattacaata gcactggcaa catgggagtg cttgcaggtc aggcctctca 18000

gttgaatgca gtggtggact tgcaagacag aaacacagaa ctgtcctacc agctcttgct 18060

tgattccatg ggtgacagaa ccagatactt ttccatgtgg aatcaggcag tggacagtta 18120

tgacccagat gttagaatta ttgaaaatca tggaactgaa gacgagctcc ccaactattg 18180

tttccctctg ggtggcatag gggtaactga cacttaccag gctgttaaaa ccaacaatgg 18240

caataacggg ggccaggtga cttggacaaa agatgaaact tttgcagatc gcaatgaaat 18300

aggggtggga aacaatttcg ctatggagat caacctcagt gccaacctgt ggagaaactt 18360

cctgtactcc aacgtggcgc tgtacctacc agacaagctt aagtacaacc cctccaatgt 18420

ggacatctct gacaacccca acacctacga ttacatgaac aagcgagtgg tggccccggg 18480

gctggtggac tgctacatca acctgggcgc gcgctggtcg ctggactaca tggacaacgt 18540

caaccccttc aaccaccacc gcaatgcggg cctgcgctac cgctccatgc tcctgggcaa 18600

cgggcgctac gtgcccttcc acatccaggt gccccagaag ttctttgcca tcaagaacct 18660

cctcctcctg ccgggctcct acacctacga gtggaacttc aggaaggatg tcaacatggt 18720

cctccagagc tctctgggta acgatctcag ggtggacggg gccagcatca agttcgagag 18780

catctgcctc tacgccacct tcttccccat ggcccacaac acggcctcca cgctcgaggc 18840

catgctcagg aacgacacca acgaccagtc cttcaatgac tacctttccg ccgccaacat 18900

gctctacccc atacccgcca acgccaccaa cgtccccatc tccatcccct cgcgcaactg 18960

ggcggccttc cgcggctggg ccttcacccg cctcaagacc aaggagaccc cctccctggg 19020

ctcgggattc gacccctact acacctactc gggctctatt ccctacctgg acggcacctt 19080

ctacctcaac cacactttca agaaggtctc ggtcaccttc gactcctcgg tcagctggcc 19140

gggcaacgac cgtctgctca cccccaacga gttcgagatc aagcgctcgg tcgacgggga 19200

aggctacaac gtggcccagt gcaacatgac caaggactgg ttcctggtcc agatgctggc 19260

caactacaac atcggctacc agggcttcta catcccagag agctacaagg acaggatgta 19320

ctccttcttc aggaacttcc agcccatgag ccggcaggtg gtggaccaga ccaagtacaa 19380

ggactaccag gaggtgggca tcatccacca gcacaacaac tcgggcttcg tgggctacct 19440

cgcccccacc atgcgcgagg gacaggccta ccccgccaac ttcccctacc cgctcatagg 19500

caagaccgcg gtcgacagca tcacccagaa aaagttcctc tgcgaccgca ccctctggcg 19560

catccccttc tccagcaact tcatgtccat gggtgcgctc tcggacctgg gccagaactt 19620

gctctacgcc aactccgccc acgccctcga catgaccttc gaggtcgacc ccatggacga 19680

gcccaccctt ctctatgttc tgttcgaagt ctttgacgtg gtccgggtcc accagccgca 19740

ccgcggcgtc atcgagaccg tgtacctgcg tacgcccttc tcggccggca acgccaccac 19800

ctaaagaagc aagccgcagt catcgccgcc tgcatgccgt cgggttccac cgagcaagag 19860

ctcagggcca tcgtcagaga cctgggatgc gggccctatt ttttgggcac cttcgacaag 19920

cgcttccctg gctttgtctc cccacacaag ctggcctgcg ccatcgtcaa cacggccggc 19980

cgcgagaccg ggggcgtgca ctggctggcc tttgcctgga acccgcgctc caaaacatgc 20040

ttcctctttg accccttcgg cttttcggac cagcggctca agcaaatcta cgagttcgag 20100

tacgagggct tgctgcgtcg cagcgccatc gcctcctcgc ccgaccgctg cgtcaccctc 20160

gaaaagtcca cccagaccgt gcaggggccc gactcggccg cctgcggtct cttctgctgc 20220

atgtttctgc acgcctttgt gcactggcct cagagtccca tggaccgcaa ccccaccatg 20280

aacttgctga cgggggtgcc caactccatg ctccaaagcc cccaggtcga gcccaccctg 20340

cgccgcaacc aggagcagct ctacagcttc ctggagcgcc actcgcccta cttccgccgc 20400

cacagcgcac agatcaggag ggccacctcc ttctgccact tgcaagagat gcaagaaggg 20460

taataacgat gtacacactt ttttctcaat aaatggcatt ttttttttat ttatacaagc 20520

tctctggggt attcatttcc caccaccacc acccgccgtt gtcgccatct ggctctattt 20580

agaaatcgaa agggttctgc cgggagtcgc cgtgcgccac gggcagggac acgttgcgat 20640

actggtagcg ggtgccccac ttgaactcgg gcaccaccag gcgaggcagc tcggggaagt 20700

tttcgctcca caggctgcgg gtcagcacca gcgcgttcat caggtcgggc gccgagatct 20760

tgaagtcgca gttggggccg ccgccctgcg cgcgcgagtt gcggtacacc gggttgcagc 20820

actggaacac caacagcgcc gggtgcttca cgctggccag cacgctgcgg tcggagatca 20880

gctcggcgtc caggtcctcc gcgttgctca gcgcgaacgg ggtcatcttg ggcacttgcc 20940

gccccaggaa gggcgcgtgc cccggtttcg agttgcagtc gcagcgcagc gggatcagca 21000

ggtgcccgtg cccggactcg gcgttggggt acagcgcgcg catgaaggcc tgcatctggc 21060

ggaaggccat ctgggccttg gcgccctccg agaagaacat gccgcaggac ttgcccgaga 21120

actggtttgc ggggcagctg gcgtcgtgca ggcagcagcg cgcgtcggtg ttggcgatct 21180

gcaccacgtt gcgcccccac cggttcttca cgatcttggc cttggacgat tgctccttca 21240

gcgcgcgctg cccgttctcg ctggtcacat ccatctcgat cacatgttcc ttgttcacca 21300

tgctgctgcc gtgcagacac ttcagctcgc cctccgtctc ggtgcagcgg tgctgccaca 21360

gcgcgcagcc cgtgggctcg aaagacttgt aggtcacctc cgcgaaggac tgcaggtacc 21420

cctgcaaaaa gcggcccatc atggtcacga aggtcttgtt gctgctgaag gtcagctgca 21480

gcccgcggtg ctcctcgttc agccaggtct tgcacacggc cgccagcgcc tccacctggt 21540

cgggcagcat cttgaagttc accttcagct cattctccac gtggtacttg tccatcagcg 21600

tgcgcgccgc ctccatgccc ttctcccagg ccgacaccag cggcaggctc acggggttct 21660

tcaccatcac cgtggccgcc gcctccgccg cgctttcgct ttccgccccg ctgttctctt 21720

cctcttcctc ctcttcctcg ccgccgccca ctcgcagccc ccgcaccacg gggtcgtctt 21780

cctgcaggcg ctgcaccttg cgcttgccgt tgcgcccctg cttgatgcgc acgggcgggt 21840

tgctgaagcc caccatcacc agcgcggcct cttcttgctc gtcctcgctg tccagaatga 21900

cctccgggga gggggggttg gtcatcctca gtaccgaggc acgcttcttt ttcttcctgg 21960

gggcgttcgc cagctccgcg gctgcggccg ctgccgaggt cgaaggccga gggctgggcg 22020

tgcgcggcac cagcgcgtct tgcgagccgt cctcgtcctc ctcggactcg agacggaggc 22080

gggcccgctt cttcgggggc gcgcggggcg gcggaggcgg cggcggcgac ggagacgggg 22140

acgagacatc gtccagggtg ggtggacggc gggccgcgcc gcgtccgcgc tcgggggtgg 22200

tttcgcgctg gtcctcttcc cgactggcca tctcccactg ctccttctcc tataggcaga 22260

aagagatcat ggagtctctc atgcgagtcg agaaggagga ggacagccta accgccccct 22320

ctgagccctc caccaccgcc gccaccaccg ccaatgccgc cgcggacgac gcgcccaccg 22380

agaccaccgc cagtaccacc ctccccagcg acgcaccccc gctcgagaat gaagtgctga 22440

tcgagcagga cccgggtttt gtgagcggag aggaggatga ggtggatgag aaggagaagg 22500

aggaggtcgc cgcctcagtg ccaaaagagg ataaaaagca agaccaggac gacgcagata 22560

aggatgagac agcagtcggg cgggggaacg gaagccatga tgctgatgac ggctacctag 22620

acgtgggaga cgacgtgctg cttaagcacc tgcaccgcca gtgcgtcatc gtctgcgacg 22680

cgctgcagga gcgctgcgaa gtgcccctgg acgtggcgga ggtcagccgc gcctacgagc 22740

ggcacctctt cgcgccgcac gtgcccccca agcgccggga gaacggcacc tgcgagccca 22800

acccgcgtct caacttctac ccggtcttcg cggtacccga ggtgctggcc acctaccaca 22860

tcttcttcca aaactgcaag atccccctct cctgccgcgc taaccgcacc cgcgccgaca 22920

aaaccctgac cctgcggcag ggcgcccaca tacctgatat tgcctctctg gaggaagtgc 22980

ccaagatctt cgagggtctc ggtcgcgacg agaaacgggc ggcgaacgct ctgcacggag 23040

acagcgaaaa cgagagtcac tcgggggtgc tggtggagct cgagggcgac aacgcgcgcc 23100

tggccgtact caagcgcagc atagaggtca cccactttgc ctacccggcg ctcaacctgc 23160

cccccaaggt catgagtgtg gtcatgggcg agctcatcat gcgccgcgct cagcccctgg 23220

ccgcggatgc aaacttgcaa gagtcctccg aggaaggcct gcccgcggtc agcgacgagc 23280

agctagcgcg ctggctggag acccgcgacc ccgcgcagct ggaggagcgg cgcaagctca 23340

tgatggccgc ggtgctggtc accgtggagc tcgagtgtct gcagcgcttc ttcgcggacc 23400

ccgagatgca gcgcaagctc gaggagaccc tgcactacac cttccgccag ggctacgtgc 23460

gccaggcctg caagatctcc aacgtggagc tctgcaacct ggtctcctac ctgggcatcc 23520

tgcacgagaa ccgcctcggg cagaacgtcc tgcactccac cctcaaaggg gaggcgcgcc 23580

gcgactacat ccgcgactgc gcctacctct tcctctgcta cacctggcag acggccatgg 23640

gggtctggca gcagtgcctg gaggagcgca acctcaagga gctggaaaag ctactcaagc 23700

gcaccctcag ggacctctgg acgggcttca acgagcgctc ggtggccgcc gcgctggcgg 23760

acatcatctt ccccgagcgc ctgctcaaga ccctgcagca gggcctgccc gacttcacca 23820

gccagagcat gctgcagaac tttaggactt tcatcctgga gcgctcgggc atcctgcctg 23880

ccacttgctg cgcgctgccc agcgacttcg tgcccatcaa gtacagggag tgcccgccgc 23940

cgctctgggg ccactgctac ctcttccagc tggccaacta cctcgcctac cactcggacc 24000

tcatggaaga cgtgagcggc gagggcctgc tcgagtgcca ctgccgctgc aacctctgca 24060

cgccccaccg ctctctagtc tgcaacccgc agctgctcag cgagagtcag attatcggta 24120

ccttcgagct gcagggtccc tcgcctgacg agaagtccgc ggctccgggg ctgaaactca 24180

ctccggggct gtggacttcc gcctacctac gcaaatttgt acctgaggac taccacgccc 24240

acgagatcag gttctacgaa gaccaatccc gcccgcccaa ggcggagctc accgcctgcg 24300

tcatcaccca ggggcacatc ctgggccaat tgcaagccat caacaaagcc cgccgagagt 24360

tcttgctgaa aaagggtcgg ggggtgtacc tggaccccca gtccggcgag gagctaaacc 24420

cgctaccccc gccgccgccc cagcagcggg accttgcttc ccaggatggc acccagaaag 24480

aagcagcagc cgccgccgcc gcagccatac atgcttctgg aggaagagga ggaggactgg 24540

gacagtcagg cagaggaggt ttcggacgag gagcaggagg agatgatgga agactgggag 24600

gaggacagca gcctagacga ggaagcttca gaggccgaag aggtggcaga cgcaacacca 24660

tcaccctcgg tcgcagcccc ctcgccgggg cccctgaaat cctccgaacc cagcaccagc 24720

gctataacct ccgctcctcc ggcgccggcg ccacccgccc gcagacccaa ccgtagatgg 24780

gacaccacag gaaccggggt cggtaagtcc aagtgcccgc cgccgccacc gcagcagcag 24840

cagcagcgcc agggctaccg ctcgtggcgc gggcacaaga acgccatagt cgcctgcttg 24900

caagactgcg ggggcaacat ctctttcgcc cggcgcttcc tgctattcca ccacggggtc 24960

gcctttcccc gcaatgtcct gcattactac cgtcatctct acagccccta ctgcagcggc 25020

gacccagagg cggcagcggc agccacagcg gcgaccacca cctaggaaga tatcctccgc 25080

gggcaagaca gcggcagcag cggccaggag acccgcggca gcagcggcgg gagcggtggg 25140

cgcactgcgc ctctcgccca acgaacccct ctcgacccgg gagctcagac acaggatctt 25200

ccccactttg tatgccatct tccaacagag cagaggccag gagcaggagc tgaaaataaa 25260

aaacagatct ctgcgctccc tcacccgcag ctgtctgtat cacaaaagcg aagatcagct 25320

tcggcgcacg ctggaggacg cggaggcact cttcagcaaa tactgcgcgc tcactcttaa 25380

agactagctc cgcgcccttc tcgaatttag gcgggagaaa actacgtcat cgccggccgc 25440

cgcccagccc gcccagccga gatgagcaaa gagattccca cgccatacat gtggagctac 25500

cagccgcaga tgggactcgc ggcgggagcg gcccaggact actccacccg catgaactac 25560

atgagcgcgg gaccccacat gatctcacag gtcaacggga tccgcgccca gcgaaaccaa 25620

atactgctgg aacaggcggc catcaccgcc acgccccgcc ataatctcaa cccccgaaat 25680

tggcccgccg ccctcgtgta ccaggaaacc ccctccgcca ccaccgtact acttccgcgt 25740

gacgcccagg ccgaagtcca gatgactaac tcaggggcgc agctcgcggg cggctttcgt 25800

cacggggcgc ggccgctccg accaggtata agacacctga tgatcagagg ccgaggtatc 25860

cagctcaacg acgagtcggt gagctcttcg ctcggtctcc gtccggacgg aactttccag 25920

ctcgccggat ccggccgctc ttcgttcacg ccccgccagg cgtacctgac tctgcagacc 25980

tcgtcctcgg agccccgctc cggaggcatc ggaaccctcc agttcgtgga ggagttcgtg 26040

ccctcggtct acttcaaccc cttctcggga cctcccggac gctaccccga ccagttcatt 26100

ccgaactttg acgcggtgaa ggactcggcg gacggctacg actgaatgtc aggtgccgag 26160

gcagagcagc ttcgcctgag acacctcgag cactgccgcc gccacaagtg cttcgcccgc 26220

ggttccggtg agttctgcta ctttcagcta cccgaggagc ataccgaggg gccggcgcac 26280

ggcgtccgcc tgaccaccca gggcgaggtt acctgttccc tcatccggga gttcaccctc 26340

cgtcccctgc tagtggagcg ggagcggggt ccctgtgtcc taactatcgc ctgcaactgc 26400

cctaaccctg gattacatca agatctttgc tgtcatctct gtgctgagtt taataaacgc 26460

tgagatcaga atctactggg gctcctgtcg ccatcctgtg aacgccaccg tcttcaccca 26520

ccccgaccag gcccaggcga acctcacctg cggtctgcat cggagggcca agaagtacct 26580

cacctggtac ttcaacggca ccccctttgt ggtttacaac agcttcgacg gggacggagt 26640

ctccctgaaa gaccagctct ccggtctcag ctactccatc cacaagaaca ccaccctcca 26700

actcttccct ccctacctgc cgggaaccta cgagtgcgtc accggccgct gcacccacct 26760

cacccgcctg atcgtaaacc agagctttcc gggaacagat aactccctct tccccagaac 26820

aggaggtgag ctcaggaaac tccccgggga ccagggcgga gacgtacctt cgacccttgt 26880

ggggttagga ttttttatta ccgggttgct ggctctttta atcaaagctt ccttgagatt 26940

tgttctttcc ttctacgtgt ggttaacccc ttaattaagg ggattccata tggtacgtac 27000

atttagtccc ctttaactaa tcaaacactg gaatcaataa aaagaatcac ttacttaaaa 27060

tcagacagca ggtctctgtc cagtttattc agcagcacct ccttcccctc ctcccaactc 27120

tggtactcca aacgccttct ggcggcaaac ttcctccaca ccctgaaggg aatgtcagat 27180

tcttgctcct gtccctccgc acccactatc ttcatgttgt tgcagatgaa gcgcaccaaa 27240

acgtctgacg agagcttcaa ccccgtgtac ccctatgaca cggaaagcgg ccctccctcc 27300

gtccctttcc tcacccctcc cttcgtgtct cccgatggat tccaagaaag cccccccggg 27360

gtcctgtctc tgaacctggc cgagcccctg gtcacttccc acggcatgct cgccctgaaa 27420

atgggaagtg gcctctccct ggacgacgct ggcaacctca cctctcaaga tatcaccacc 27480

gctagccctc ccctcaaaaa aaccaagacc aacctcagcc tagaaacctc atccccccta 27540

actgtaagca cctcaggcgc cctcaccgta gcagccgccg ctcccctggc agtggccggc 27600

acctccctca ccatgcaatc agaggccccc ctgacagtac aggatgcaaa actcaccctg 27660

gccaccaaag gccccctgac cgtgtctgaa ggcaaactgg ccttgcaaac atcggccccg 27720

ctgacggccg ctgacagcag caccctcacc gttagcgcca caccaccaat taatgtaagc 27780

agtggaagtt taggcttaga catggaagac cctatgtata ctcacgatgg aaaactggga 27840

ataagaattg ggggtccact aagagtagta gacagcttgc acacactcac tgtagttacc 27900

ggaaatggac taactgtaga taacaatgcc ctccaaacta gagttacggg cgccctaggt 27960

tatgacacat caggaaatct acaattgaga gctgcaggag gtatgcgaat tgatgcaaat 28020

ggccaactta tccttaatgt ggcataccca tttgatgctc agaacaatct cagccttaga 28080

cttggtcagg gacccctgta tataaacaca gaccacaacc tggatttgaa ttgcaacaga 28140

ggtctaacca caactaccac caacaacaca aaaaaacttg agactaaaat tagctcaggc 28200

ttagactatg acaccaatgg tgctgtcatt attaaacttg gcactggtct aagcttcgac 28260

aacacaggcg ccctaactgt gggaaacact ggtgatgata aactgactct gtggacgacc 28320

ccagacccat ctccaaattg cagaattcac tcagacaaag actgcaagtt tactctagtc 28380

ctaactaagt gtggaagcca aatcctggcc tctgtcgccg ccctagcggt atcaggaaat 28440

ctggcttcga taacaggcac cgttgccagc gttaccatct ttctcagatt tgatcagaat 28500

ggagtgctta tggaaaactc ctcgctagac aggcagtact ggaacttcag aaatggcaac 28560

tcaactaacg ctgcccccta caccaatgca gttgggttca tgccaaacct cgcagcatac 28620

cccaaaacgc aaagccagac tgctaaaaac aacattgtaa gtcaggttta cttgaatgga 28680

gacaaatcca aacccatgac ccttaccatc accctcaatg gaactaatga atccagtgaa 28740

actagccagg tgagtcacta ctccatgtca tttacatggg cttgggaaag tgggcaatat 28800

gccactgaaa cctttgccac caactccttc accttttctt acattgctga acaataaaaa 28860

gcatgacact gatgttcatt tctgattctt attttattat tttcaaacac aacaaaatca 28920

ttcaagtcat tcttccatct tagcttaata gacacagtag cttaatagac ccagtagtgc 28980

aaagccccat tctagcttat aactagtgga gaagtactcg cctattatct ggaagaagca 29040

gaagagcggc ggtgggaatc atagtccgcg aacgggatcg gccggtggtg tcgcatcagg 29100

ccccgcagca gtcgctgccg ccgccgctcc gtcaagctgc tgctcagggg gtccgggtcc 29160

agggactccc tcagcatgat gcccacggcc ctcagcatca gtcgtctggt gcggcgggcg 29220

cagcagcgca tgcggatctc gctcaggtcg ctgcagtacg tgcaacacag aaccaccagg 29280

ttgttcaaca gtccatagtt caacacgctc cagccgaaac tcatcgcggg aaggatgcta 29340

cccacgtggc cgtcgtacca gatcctcagg taaatcaagt ggtgccccct ccagaacacg 29400

ctgcccacgt acatgatctc cttgggcatg tggcggttca ccacctcccg gtaccacatc 29460

accctctggt tgaacatgca gccccggatg atcctgcgga accacagggc cagcaccgcc 29520

ccgcccgcca tgcagcgaag agaccccggg tcccggcaat ggcaatggag gacccaccgc 29580

tcgtacccgt ggatcatctg ggagctgaac aagtctatgt tggcacagca caggcatatg 29640

ctcatgcatc tcttcagcac tctcaactcc tcgggggtca aaaccatatc ccagggcacg 29700

gggaactctt gcaggacagc gaaccccgca gaacagggca atcctcgcac agaacttaca 29760

ttgtgcatgg acagggtatc gcaatcaggc agcaccgggt gatcctccac cagagaagcg 29820

cgggtctcgg tctcctcaca gcgtggtaag ggggccggcc gatacgggtg atggcgggac 29880

gcggctgatc gtgttcgcga ccgtgtcatg atgcagttgc tttcggacat atttcctgaa 29940

gtcttagatc tctcaacgca gcaccagcac caacacttcg cagtgtaaaa ggccaagtgc 30000

cgagagagta tatataggaa taaaaagtga cgtaaacggg caaagtccaa aaaacgccca 30060

gaaaaaccgc acgcgaacct acgccccgaa acgaaagcca aaaaacacta gacactccct 30120

tccggcgtca acttccgctt tcccacgcta cgtcacttgc cccagtcaaa caaactacat 30180

atcccgaact tccaagtcgc cacgcccaaa acaccgccta cacctccccg cccgccggcc 30240

cgcccccaaa cccgcctccc gccccgcgcc ccgccccgcg ccgcccatct cattatcata 30300

ttggcttcaa tccaaaataa ggtatattat tgatgatgat ttaaatggcg cgccgggccc 30360

gggccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg 30420

cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt 30480

gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc 30540

tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc 30600

ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc 30660

cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga 30720

gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt 30780

ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag 30840

tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag 30900

aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta 30960

ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg 31020

agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca 31080

gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag 31140

gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc 31200

gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg 31260

tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc 31320

ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg 31380

cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg 31440

gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga 31500

cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac 31560

tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa 31620

aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca 31680

aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 31740

gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 31800

cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 31860

ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc 31920

accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 31980

tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 32040

cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 32100

gaacgaccta caccgaactg agatacctac agcgtgagct ttgagatagc gccacgcttc 32160

ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 32220

cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 32280

tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 32340

ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct 32400

ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata 32460

ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc 32520

gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg 32580

acaggtttcc cgactggatt t 32601

<210> 12

<211> 34587

<212> DNA

<213> Artificial sequence

<220>

<223> chimpanzee ChAd3 type adenovirus vector plasmid sequence for recombinant expression of EGFP (pChAd3-EGFP)

<400> 12

aaatcatcat caataatata ccttattttg gattgaagcc aatatgataa tgagatgggc 60

ggcgcgaggc ggggcgcggg gcgggaggcg ggtttggggg cgggccggcg ggcggggcgg 120

tgtggcggaa gtggactttg taagtgtggc ggatgtgact tgctagtgcc gggcgcggta 180

aaagtgacgt tttccgtgcg cgacaacgcc cccgggaagt gacatttttc ccgcggtttt 240

taccggatgt tgtagtgaat ttgggcgtaa ccaagtaaga tttggccatt ttcgcgggaa 300

aactgaaacg gggaagtgaa atctgattaa ttttgcgtta gtcataccgc gtaatatttg 360

tctagggccg agggactttg gccgattacg tggaggactc gcccaggtgt tttttgaggt 420

gaatttccgc gttccgggtc aaagtctccg ttttattatt atagtcagct gacgcggagt 480

gtatttatac cctctgatct cgtcaagagg ccactcttga gtgccagcga gtagagtttt 540

ctcctctgcc gctctccgct ccgctccgct cggctctgac accggggaaa aagcgatcgc 600

cgatatcatt tccccgaaaa gtgccacctg accgtaacta taacggtcct aaggtagcga 660

aagctcagat ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca 720

tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc 780

aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg 840

gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta 900

ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 960

ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 1020

ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 1080

cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 1140

atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 1200

cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 1260

attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 1320

cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 1380

caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 1440

cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcactaga 1500

agctttattg cggtagttta tcacagttaa attgctaacg cagtcagtgc ttctgacaca 1560

acagtctcga acttaagctg cagaagttgg tcgtgaggca ctgggcaggt aagtatcaag 1620

gttacaagac aggtttaagg agaccaatag aaactgggct tgtcgagaca gagaagactc 1680

ttgcgtttct gataggcacc tattggtctt actgacatcc actttgcctt tctctccaca 1740

ggtgtccact cccagttcaa ttacagctct taaggctaga gtactctagc ccgggatcca 1800

ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 1860

gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 1920

gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg 1980

ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc 2040

gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 2100

cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 2160

ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 2220

atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac 2280

aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc 2340

gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 2400

cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc 2460

gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 2520

ctgtacaagt aaagcggccg ccactgtgct ggatgatccg agctcggtac ctctagagtc 2580

gacccgggcg gccaaaccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg 2640

ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt 2700

cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg 2760

gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg 2820

atgcggtggg ctctatggct tctgaggcgg aaagaaccag cagatctgca gatctgaatt 2880

catctatgtc gggtgcggag aaagaggtaa tgaaatggca ttatgggtat tatgggtctg 2940

cattaatgaa tcggccagat atcgacatat gctggccacc gtacatgtgg cttcccatgc 3000

tcgcaagccc tggcccgagt tcgagcacaa tgtcatgacc aggtgcaata tgcatctggg 3060

gtcccggcga tcgcgcgggg ggtgttcctg ccctatcaat gcaactttag tcacaccaag 3120

atcttgctag agcccgagag catgtccaag gtgaacttga acggggtgtt tgacatgacc 3180

atgaagatct ggaaggtgct gaggtacgac gagaccaggt cccggtgcag accctgcgag 3240

tgcgggggca agcatatgag gaaccagccc gtgatgctgg atgtgaccga ggagctgagg 3300

acagaccact tggttctggc ctgcaccagg gccgagtttg gttctagcga tgaagacaca 3360

gattgaggtg ggtgagtggg cgtggcctgg ggtggtcatg aaaatatata agttgggggt 3420

cttagggtct ctttatttgt gttgcagaga ccgccggagc catgagcggg agcagcagca 3480

gcagcagtag cagcagcgcc ttggatggca gcatcgtgag cccttatttg acgacgcgga 3540

tgccccactg ggccggggtg cgtcagaatg tgatgggctc cagcatcgac ggccgacccg 3600

tcctgcccgc aaattccgcc acgctgacct atgcgaccgt cgcggggacg ccgttggacg 3660

ccaccgccgc cgccgccgcc accgcagccg cctcggccgt gcgcagcctg gccacggact 3720

ttgcattcct gggaccactg gcgacagggg ctacttctcg ggccgctgct gccgccgttc 3780

gcgatgacaa gctgaccgcc ctgctggcgc agttggatgc gcttactcgg gaactgggtg 3840

acctttctca gcaggtcatg gccctgcgcc agcaggtctc ctccctgcaa gctggcggga 3900

atgcttctcc cacaaatgcc gtttaagata aataaaacca gactctgttt ggattaaaga 3960

aaagtagcaa gtgcattgct ctctttattt cataattttc cgcgcgcgat aggccctaga 4020

ccagcgttct cggtcgttga gggtgcggtg tatcttctcc aggacgtggt agaggtggct 4080

ctggacgttg agatacatgg gcatgagccc gtcccggggg tggaggtagc accactgcag 4140

agcttcatgc tccggggtgg tgttgtagat gatccagtcg tagcaggagc gctgggcatg 4200

gtgcctaaaa atgtccttca gcagcaggcc gatggccagg gggaggccct tggtgtaagt 4260

gtttacaaaa cggttaagtt gggaagggtg cattcgggga gagatgatgt gcatcttgga 4320

ctgtattttt agattggcga tgtttccgcc cagatccctt ctgggattca tgttgtgcag 4380

gaccaccagt acagtgtatc cggtgcactt ggggaatttg tcatgcagct tagagggaaa 4440

agcgtggaag aacttggaga cgcccttgtg gcctcccaga ttttccatgc attcgtccat 4500

gatgatggca atgggcccgc gggaggcagc ttgggcaaag atatttctgg ggtcgctgac 4560

gtcgtagttg tgttccaggg tgaggtcgtc ataggccatt tttacaaagc gcgggcggag 4620

ggtgcccgac tgggggatga tggtcccctc tggccctggg gcgtagttgc cctcgcagat 4680

ctgcatttcc caggccttaa tctcggaggg gggaatcata tccacctgcg gggcgatgaa 4740

gaaaacggtt tccggagccg gggagattaa ctgggatgag agcaggtttc taagcagctg 4800

tgattttcca caaccggtgg gcccataaat aacacctata accggttgca gctggtagtt 4860

tagagagctg cagctgccgt cgtcccggag gaggggggcc acctcgttga gcatgtccct 4920

gacgcgcatg ttctccccga ccagatccgc cagaaggcgc tcgccgccca gggacagcag 4980

ctcttgcaag gaagcaaagt ttttcagcgg cttgaggccg tccgccgtgg gcatgttttt 5040

cagggtctgg ctcagcagct ccaggcggtc ccagagctcg gtgacgtgct ctacggcatc 5100

tctatccagc atatctcctc gtttcgcggg ttggggcgac tttcgctgta gggcaccaag 5160

cggtggtcgt ccagcggggc caaagtcatg tccttccatg ggcgcagggt cctcgtcagg 5220

gtggtctggg tcacggtgaa ggggtgcgct ccgggctgag cgcttgccaa ggtgcgcttg 5280

aggctggttc tgctggtgct gaagcgctgc cggtcttcgc cctgcgcgtc ggccaggtag 5340

catttgacca tggtgtcata gtccagcccc tccgcggcgt gtcccttggc gcgcagcttg 5400

cccttggagg tggcgccgca cgaggggcag agcaggctct tgagcgcgta gagcttgggg 5460

gcgaggaaga ccgattcggg ggagtaggcg tccgcgccgc agaccccgca cacggtctcg 5520

cactccacca gccaggtgag ctcggggcgc gccgggtcaa aaaccaggtt tcccccatgc 5580

tttttgatgc gtttcttacc tcgggtctcc atgaggtggt gtccccgctc ggtgacgaag 5640

aggctgtccg tgtctccgta gaccgacttg aggggtcttt tctccagggg ggtccctcgg 5700

tcttcctcgt agaggaactc ggaccactct gagacgaagg cccgcgtcca ggccaggacg 5760

aaggaggcta tgtgggaggg gtagcggtcg ttgtccacta gggggtccac cttctccaag 5820

gtgtgaagac acatgtcgcc ttcctcggcg tccaggaagg tgattggctt gtaggtgtag 5880

gccacgtgac cgggggttcc tgacgggggg gtataaaagg gggtgggggc gcgctcgtcg 5940

tcactctctt ccgcatcgct gtctgcgagg gccagctgct ggggtgagta ttccctctcg 6000

aaggcgggca tgacctccgc gctgaggttg tcagtttcca aaaacgagga ggatttgatg 6060

ttcacctgtc ccgaggtgat acctttgagg gtacccgcgt ccatctggtc agaaaacacg 6120

atctttttat tgtccagctt ggtggcgaac gacccgtaga gggcgttgga gagcagcttg 6180

gcgatggagc gcagggtctg gttcttgtcc ctgtcggcgc gctccttggc cgcgatgttg 6240

agctgcacgt actcgcgcgc gacgcagcgc cactcgggga agacggtggt gcgctcgtcg 6300

ggcaccaggc gcacgcgcca gccgcggttg tgcagggtga ccaggtccac gctggtggcg 6360

acctcgccgc gcaggcgctc gttggtccag cagagacggc cgcccttgcg cgagcagaag 6420

gggggcaggg ggtcgagctg ggtctcgtcc ggggggtccg cgtccacggt gaaaaccccg 6480

gggcgcaggc gcgcgtcgaa gtagtctatc ttgcaacctt gcatgtccag cgcctgctgc 6540

cagtcgcggg cggcgagcgc gcgctcgtag gggttgagcg gcgggcccca gggcatgggg 6600

tgggtgagtg cggaggcgta catgccgcag atgtcataga cgtagagggg ctcccgcagg 6660

accccgatgt aggtggggta gcagcggccg ccgcggatgc tggcgcgcac gtagtcatac 6720

agctcgtgcg agggggcgag gaggtcgggg cccaggttgg tgcgggcggg gcgctccgcg 6780

cggaagacga tctgcctgaa gatggcatgc gagttggaag agatggtggg gcgctggaag 6840

acgttgaagc tggcgtcctg caggccgacg gcgtcgcgca cgaaggaggc gtaggagtcg 6900

cgcagcttgt gtaccagctc ggcggtgacc tgcacgtcga gcgcgcagta gtcgagggtc 6960

tcgcggatga tgtcatattt agcctgcccc ttctttttcc acagctcgcg gttgaggaca 7020

aactcttcgc ggtctttcca gtactcttgg atcgggaaac cgtccggttc cgaacggtaa 7080

gagcctagca tgtagaactg gttgacggcc tggtaggcgc agcagccctt ctccacgggg 7140

agggcgtagg cctgcgcggc cttgcggagc gaggtgtggg tcagggcgaa ggtgtccctg 7200

accatgactt tgaggtactg gtgcttgaag tcggagtcgt cgcagccgcc ccgctcccag 7260

agcgagaagt cggtgcgctt cttggagcgg gggttgggca gagcgaaggt gacatcgttg 7320

aagaggattt tgcccgcgcg gggcatgaag ttgcgggtga tgcggaaggg ccccggcact 7380

tcagagcggt tgttgatgac ctgggcggcg agcacgatct cgtcgaagcc gttgatgttg 7440

tggcccacga tgtagagttc caggaagcgg ggccggccct ttacggtggg cagcttcttt 7500

agctcttcgt aggtgagctc ctcgggcgag gcgaggccgt gctcggccag ggcccagtcc 7560

gcgaggtgcg ggttgtctct gaggaaggac tcccagaggt cgcgggccag gagggtctgc 7620

aggcggtccc tgaaggtcct gaactggcgg cccacggcca ttttttcggg ggtgatgcag 7680

tagaaggtga gggggtcttg ctgccagcgg tcccagtcga gctgcagggc gaggtcgcgc 7740

gcggcggtga ccaggcgctc gtcgcccccg aatttcatga ccagcatgaa gggcacgagc 7800

tgctttccga aggcccccat ccaagtgtag gtctctacat cgtaggtgac aaagaggcgc 7860

tccgtgcgag gatgcgagcc gatcgggaag aactggatct cccgccacca gttggaggag 7920

tggctgttga tgtggtggaa gtagaagtcc cgtcgccggg ccgaacactc gtgctggctt 7980

ttgtaaaagc gagcgcagta ctggcagcgc tgcacgggct gtacctcctg cacgagatgc 8040

acctttcgcc cgcgcacgag gaagccgagg ggaaatctga gccccccgcc tggctcgcgg 8100

catggctggt gctcttctac tttggatgcg tgtccgtctc cgtctggctc ctcgaggggt 8160

gttacggtgg agcggaccac cacgccgcgc gagccgcagg tccagatatc ggcgcgcggc 8220

ggtcggagtt tgatgacgac atcgcgcagc tgggagctgt ccatggtctg gagctcccgc 8280

ggcggcggca ggtcagccgg gagttcttgc aggttcacct cgcagagtcg ggccagggcg 8340

cggggcaggt ctaggtggta cctgatctct aggggcgtgt tggtggcggc gtcgatggct 8400

tgcaggagcc cgcatccccg gggggcgacg acggtgcccc gcggggtggt ggtggtggtg 8460

gtggtggtgg tggtggcggt gcagctcaga agcggtgccg cgggcgggcc cccggaggta 8520

gggggggctc cggtcccgcc ggcaggggcg gcagcggcac gtcggcgtgg agcgcgggca 8580

ggagttggtg ctgtgcccgg aggttgctgg cgaaggcgac gacgcggcgg ttgatctcct 8640

ggatctggcg cctctgcgtg aagacgacgg gcccggtgag cttgaacctg aaagagagtt 8700

cgacagaatc aatctcggtg tcattgaccg cggcctggcg caggatctcc tgcacgtctc 8760

ccgagttgtc ttggtaggcg atctcggcca tgaactgctc gatctcttcc tcctggaggt 8820

ctccgcgtcc ggcgcgttcc acggtggccg ccaggtcgtt ggagatgcgc cccatgagct 8880

gcgagaaggc gttgagtccg ccctcgttcc agactcggct gtagaccacg cccccctggt 8940

catcgcgggc gcgcatgacc acctgcgcga ggttgagctc cacgtgccgc gcgaagacgg 9000

cgtagttgcg cagacgctgg aagaggtagt tgagggtggt ggcggtgtgc tcggccacga 9060

agaagttcat gacccagcgg cgcaacgtgg attcgttgat gtcccccaag gcctccagcc 9120

gttccatggc ctcgtagaag tccacggcga agttgaaaaa ctgggagttg cgcgccgaca 9180

cggtcaactc ctcctccaga agacggatga gctcggcgac ggtgtcgcgc acctcgcgct 9240

cgaaggctat ggggatctct tcctccgcta gcatcaccac ctcctcctct tcctcctctt 9300

ctggcacttc catgatggct tcctcctctt cggggggcgg cggcggcggc ggtgggggag 9360

ggggcgctct gcgccggcgg cggcgcaccg ggaggcggtc cacgaagcgc gcgatcatct 9420

ccccgcggcg gcggcgcatg gtctcggtga cggcgcggcc gttctcccgg gggcgcagtt 9480

ggaagacgcc gccggacatc tggtgctggg gcgggtggcc gtgaggcagc gaaacggcgc 9540

tgacgatgca tctcaacaat tgctgcgtag gtacgccgcc gagggacctg agggagtcca 9600

tatccaccgg atccgaaaac ctttcgagga aggcgtctaa ccagtcgcag tcgcaaggta 9660

ggctgagcac cgtggcgggc ggcggggggt ggggggagtg tctggcggag gtgctgctga 9720

tgatgtaatt gaagtaggcg gacttgacac ggcggatggt cgacaggagc accatgtcct 9780

tgggtccggc ctgctggatg cggaggcggt cggctatgcc ccaggcttcg ttctggcatc 9840

ggcgcaggtc cttgtagtag tcttgcatga gcctttccac cggcacctct tctccttcct 9900

cttctgcttc ttccatgtct gcttcggccc tggggcggcg ccgcgccccc ctgcccccca 9960

tgcgcgtgac cccgaacccc ctgagcggtt ggagcagggc caggtcggcg acgacgcgct 10020

cggccaggat ggcctgctgc acctgcgtga gggtggtttg gaagtcatcc aagtccacga 10080

agcggtggta ggcgcccgtg ttgatggtgt aggtgcagtt ggccatgacg gaccagttga 10140

cggtctggtg gcccggttgc gacatctcgg tgtacctgag tcgcgagtag gcgcgggagt 10200

cgaagacgta gtcgttgcaa gtccgcacca ggtactggta gcccaccagg aagtgcggcg 10260

gcggctggcg gtagaggggc cagcgcaggg tggcgggggc tccgggggcc aggtcttcca 10320

gcatgaggcg gtggtaggcg tagatgtacc tggacatcca ggtgataccc gcggcggtgg 10380

tggaggcgcg cgggaagtcg cgcacccggt tccagatgtt gcgcaggggc agaaagtgct 10440

ccatggtagg cgtgctctgt ccagtcagac gcgcgcagtc gttgatactc tagaccaggg 10500

aaaacgaaag ccggtcagcg ggcactcttc cgtggtctgg tgaatagatc gcaagggtat 10560

catggcggag ggcctcggtt cgagccccgg gtccgggccg gacggtccgc catgatccac 10620

gcggttaccg cccgcgtgtc gaacccaggt gtgcgacgtc agacaacggt ggagtgttcc 10680

ttttggcgtt tttctggccg ggcgccggcg tcgcgtaaga gactaagccg cgaaagcgaa 10740

agcagtaagt ggctcgctcc ccgtagccgg agggatcctt gctaagggtt gcgttgcggc 10800

gaaccccggt tcgaatcccg tactcgggcc ggccggaccc gcggctaagg tgttggattg 10860

gcctccccct cgtataaaga ccccgcttgc ggattgactc cggacacggg gacgagcccc 10920

ttttattttt gctttcccca gatgcatccg gtgctgcggc agatgcgccc cccgccccag 10980

cagcagcaac aacaccagca agagcggcag caacagcagc gggagtcatg cagggccccc 11040

tcacccaccc tcggcgggcc ggccacctcg gcgtccgcgg ccgtgtctgg cgcctgcggc 11100

ggcggcgggg ggccggctga cgaccccgag gagcccccgc ggcgcagggc cagacactac 11160

ctggacctgg aggagggcga gggcctggcg cggctggggg cgccgtctcc cgagcgccac 11220

ccgcgggtgc agctgaagcg cgactcgcgc gaggcgtacg tgcctcggca gaacctgttc 11280

agggaccgcg cgggcgagga gcccgaggag atgcgggaca ggaggttcag cgcagggcgg 11340

gagctgcggc aggggctgaa ccgcgagcgg ctgctgcgcg aggaggactt tgagcccgac 11400

gcgcggacgg ggatcagccc cgcgcgcgcg cacgtggcgg ccgccgacct ggtgacggcg 11460

tacgagcaga cggtgaacca ggagatcaac ttccaaaaga gtttcaacaa ccacgtgcgc 11520

acgctggtgg cgcgcgagga ggtgaccatc gggctgatgc acctgtggga ctttgtaagc 11580

gcgctggtgc agaaccccaa cagcaagcct ctgacggcgc agctgttcct gatagtgcag 11640

cacagcaggg acaacgaggc gtttagggac gcgctgctga acatcaccga gcccgagggt 11700

cggtggctgc tggacctgat taacatcctg cagagcatag tggtgcagga gcgcagcctg 11760

agcctggccg acaaggtggc ggccatcaac tactcgatgc tgagcctggg caagttttac 11820

gcgcgcaaga tctaccagac gccgtacgtg cccatagaca aggaggtgaa gatcgacggt 11880

ttttacatgc gcatggcgct gaaggtgctc accctgagcg acgacctggg cgtgtaccgc 11940

aacgagcgca tccacaaggc cgtgagcgtg agccggcggc gcgagctgag cgaccgcgag 12000

ctgatgcaca gcctgcagcg ggcgctggcg ggcgccggca gcggcgacag ggaggcggag 12060

tcctacttcg atgcgggggc ggacctgcgc tgggcgccca gccggcgggc cctggaggcc 12120

gcgggggtcc gcgaggacta tgacgaggac ggcgaggagg atgaggagta cgagctagag 12180

gagggcgagt acctggacta aaccgcgggt ggtgtttccg gtagatgcaa gacccgaacg 12240

tggtggaccc ggcgctgcgg gcggctctgc agagccagcc gtccggcctt aactcctcag 12300

acgactggcg acaggtcatg gaccgcatca tgtcgctgac ggcgcgtaac ccggacgcgt 12360

tccggcagca gccgcaggcc aacaggctct ccgccatcct ggaggcggtg gtgcctgcgc 12420

gctcgaaccc cacgcacgag aaggtgctgg ccatagtgaa cgcgctggcc gagaacaggg 12480

ccatccgccc ggacgaggcc gggctggtgt acgacgcgct gctgcagcgc gtggcccgct 12540

acaacagcgg caacgtgcag accaacctgg accggctggt gggggacgtg cgcgaggcgg 12600

tggcgcagcg cgagcgcgcg gatcggcagg gcaacctggg ctccatggtg gcgctgaatg 12660

ccttcctgag cacgcagccg gccaacgtgc cgcgggggca ggaagactac accaactttg 12720

tgagcgcgct gcggctgatg gtgaccgaga ccccccagag cgaggtgtac cagtcgggcc 12780

cggactactt cttccagacc agcagacagg gcctgcagac ggtgaacctg agccaggctt 12840

tcaagaacct gcgggggctg tggggcgtga aggcgcccac cggcgaccgg gcgacggtgt 12900

ccagcctgct gacgcccaac tcgcgcctgc tgctgctgct gatcgcgccg ttcacggaca 12960

gcggcagcgt gtcccgggac acctacctgg ggcacctgct gaccctgtac cgcgaggcca 13020

tcgggcaggc gcaggtggac gagcacacct tccaggagat caccagcgtg agccgcgcgc 13080

tggggcagga ggacacgagc agcctggagg cgactctgaa ctacctgctg accaaccggc 13140

ggcagaagat tccctcgctg cacagcctga cctccgagga ggagcgcatc ttgcgctacg 13200

tgcagcagag cgtgagcctg aacctgatgc gcgacggggt gacgcccagc gtggcgctgg 13260

acatgaccgc gcgcaacatg gaaccgggca tgtacgccgc gcaccggcct tacatcaacc 13320

gcctgatgga ctacctgcat cgcgcggcgg ccgtgaaccc cgagtacttt accaacgcca 13380

tcctgaaccc gcactggctc ccgccgcccg ggttctacag cgggggcttc gaggtcccgg 13440

aggccaacga tggcttcctg tgggacgaca tggacgacag cgtgttctcc ccgcggccgc 13500

aggcgctggc ggaagcgtcc ctgctgcgtc ccaagaagga ggaggaggag gcgagtcgcc 13560

gccgcggcag cagcggcgtg gcttctctgt ccgagctggg ggcggcagcc gccgcgcgcc 13620

ccgggtccct gggcggcagc ccctttccga gcctggtggg gtctctgcac agcgagcgca 13680

ccacccgccc tcggctgctg ggcgaggacg agtacctgaa taactccctg ctgcagccgg 13740

tgcgggagaa aaacctgccc cccgccttcc ccaacaacgg gatagagagc ctggtggaca 13800

agatgagcag atggaagacc tatgcgcagg agcacaggga cgcgcccgcg ctccggccgc 13860

ccacgcggcg ccagcgccac gaccggcagc gggggctggt gtgggatgac gaggactccg 13920

cggacgatag cagcgtgctg gacctgggag ggagcggcaa cccgttcgcg cacctgcgcc 13980

cccgcctggg gaggatgttt taaaaaaaaa aaaagcaaga agcatgatgc aaaattaaat 14040

aaaactcacc aaggccatgg cgaccgagcg ttggtttctt gtgttccctt cagtatgcgg 14100

cgcgcggcga tgtaccagga gggacctcct ccctcttacg agagcgtggt gggcgcggcg 14160

gcggcggcgc cctcttctcc ctttgcgtcg cagctgctgg agccgccgta cgtgcctccg 14220

cgctacctgc ggcctacggg ggggagaaac agcatccgtt actcggagct ggcgcccctg 14280

ttcgacacca cccgggtgta cctggtggac aacaagtcgg cggacgtggc ctccctgaac 14340

taccagaacg accacagcaa ttttttgacc acggtcatcc agaacaatga ctacagcccg 14400

agcgaggcca gcacccagac catcaatctg gatgaccggt cgcactgggg cggcgacctg 14460

aaaaccatcc tgcacaccaa catgcccaac gtgaacgagt tcatgttcac caataagttc 14520

aaggcgcggg tgatggtgtc gcgctcgcac accaaggaag accgggtgga gctgaagtac 14580

gagtgggtgg agttcgagct gccagagggc aactactccg agaccatgac cattgacctg 14640

atgaacaacg cgatcgtgga gcactatctg aaagtgggca ggcaaaacgg ggtcctggag 14700

agcgacatcg gggtcaagtt cgacaccagg aacttccgcc tggggctgga ccccgtgacc 14760

gggctggtta tgcccggggt gtacaccaac gaggccttcc atcccgacat catcctgctg 14820

cccggctgcg gggtggactt cacttacagc cgcctgagca acctcctggg catccgcaag 14880

cggcagccct tccaggaggg cttcaggatc acctacgagg acctggaggg gggcaacatc 14940

cccgcgctcc tcgatgtgga ggcctaccag gatagcttga aggaaaatga ggcgggacag 15000

gaggatacca cccccgccgc ctccgccgcc gccgagcagg gcgaggatgc tgctgacacc 15060

gcggccgcgg acggggcaga ggccgacccc gctatggtgg tggaggctcc cgagcaggag 15120

gaggatatga atgacagtgc ggtgcgcgga gacaccttcg tcacccgggg ggaggaaaag 15180

caagcggagg ccgaggccgc ggccgaggaa aagcaactgg cggcagcagc ggcggcggcg 15240

gcgttggccg cggcggaggc tgagtctgag gggaccaagc ccgccaagga gcccgtgatt 15300

aagcccctga ccgaagatag caagaagcgc agttacaacc tgctcaagga cagcaccaac 15360

accgcgtacc gcagctggta cctggcctac aactacggcg acccgtcgac gggggtgcgc 15420

tcctggaccc tgctgtgcac gccggacgtg acctgcggct cggagcaggt gtactggtcg 15480

ctgcccgaca tgatgcaaga ccccgtgacc ttccgctcca cgcggcaggt cagcaacttc 15540

ccggtggtgg gcgccgagct gctgcccgtg cactccaaga gcttctacaa cgaccaggcc 15600

gtctactccc agctcatccg ccagttcacc tctctgaccc acgtgttcaa tcgctttcct 15660

gagaaccaga ttctggcgcg cccgcccgcc cccaccatca ccaccgtcag tgaaaacgtt 15720

cctgctctca cagatcacgg gacgctaccg ctgcgcaaca gcatcggagg agtccagcga 15780

gtgaccgtta ctgacgccag acgccgcacc tgcccctacg tttacaaggc cttgggcata 15840

gtctcgccgc gcgtcctttc cagccgcact ttttgagcaa caccaccatc atgtccatcc 15900

tgatctcacc cagcaataac tccggctggg gactgctgcg cgcgcccagc aagatgttcg 15960

gaggggcgag gaagcgttcc gagcagcacc ccgtgcgcgt gcgcgggcac ttccgcgccc 16020

cctggggagc gcacaaacgc ggccgcgcgg ggcgcaccac cgtggacgac gccatcgact 16080

cggtggtgga gcaggcgcgc aactacaggc ccgcggtctc taccgtggac gcggccatcc 16140

agaccgtggt gcggggcgcg cggcggtacg ccaagctgaa gagccgccgg aagcgcgtgg 16200

cccgccgcca ccgccgccga cccggggccg ccgccaaacg cgccgccgcg gccctgcttc 16260

gccgggccaa gcgcacgggc cgccgcgccg ccatgagggc cgcgcgccgc ttggccgccg 16320

gcatcaccgc cgccaccatg gccccccgta cccgaagacg cgcggccgcc gccgccgccg 16380

ccgccatcag tgacatggcc agcaggcgcc ggggcaacgt gtactgggtg cgcgactcgg 16440

tgaccggcac gcgcgtgccc gtgcgcttcc gccccccgcg gacttgagat gatgtgaaaa 16500

aacaacactg agtctcctgc tgttgtgtgt atcccagcgg cggcggcgcg cgcagcgtca 16560

tgtccaagcg caaaatcaaa gaagagatgc tccaggtcgt cgcgccggag atctatgggc 16620

ccccgaagaa ggaagagcag gattcgaagc cccgcaagat aaagcgggtc aaaaagaaaa 16680

agaaagatga tgacgatgcc gatggggagg tggagttcct gcgcgccacg gcgcccaggc 16740

gcccggtgca gtggaagggc cggcgcgtaa agcgcgtcct gcgccccggc accgcggtgg 16800

tcttcacgcc cggcgagcgc tccacccgga ctttcaagcg cgtctatgac gaggtgtacg 16860

gcgacgaaga cctgctggag caggccaacg agcgcttcgg agagtttgct tacgggaagc 16920

gtcagcgggc gctggggaag gaggacctgc tggcgctgcc gctggaccag ggcaacccca 16980

cccccagtct gaagcccgtg accctgcagc aggtgctgcc gagcagcgca ccctccgagg 17040

cgaagcgggg tctgaagcgc gagggcggcg acctggcgcc caccgtgcag ctcatggtgc 17100

ccaagcggca gaggctggag gatgtgctgg agaaaatgaa agtagacccc ggtctgcagc 17160

cggacatcag ggtccgtccc atcaagcagg tggcgccggg cctcggcgtg cagaccgtgg 17220

acgtggtcat ccccaccggc aactcccccg ccgccaccac cactaccgct gcctccacgg 17280

acatggagac acagaccgat cccgccgcag ccgcagccgc cgccgcagcc gcgacctcct 17340

cggcggaggt gcagacggac ccctggctgc cgccggcgat gtcagctccc cgcgcgcgcc 17400

gcggacgcag aaagtacggc gccgccaacg cgctcctgcc cgagtacgcc ttgcatcctt 17460

ccatcgcgcc cacccccggc taccgaggct atacctaccg cccgcgaaga gccaagggtt 17520

ccacccgccg tccccgccga cgcgccgccg ccaccacccg ccgccgccgc cgcagacgcc 17580

agcccgcact ggctccagtc tccgtgagga gagtggcgcg cgacggacac accctggtgc 17640

tgcccagggc gcgctaccac cccagcatcg tttaaaagcc tgttgtggtt cttgcagata 17700

tggccctcac ttgccgcctc cgtttcccgg tgccgggata ccgaggagga agatcgcgcc 17760

gcaggagggg tctggccggc cgcggcctga gcggaggcag ccgccgcgcg caccggcggc 17820

gacgcgccac cagccgacgc atgcgcggcg gggtgctgcc cctgttaatc cccctgatcg 17880

ccgcggcgat cggcgccgtg cccgggatcg cctccgtggc cttgcaagcg tcccagaggc 17940

attgacagac ttgcaaactt gcaaatatgg aaaaaaaaaa aaaaccccaa taaaaagtct 18000

agactctcac gctcgcttgg tcctgtgact attttgtaga atggaagaca tcaactttgc 18060

gtcgctggcc ccgcgtcacg gctcgcgccc gttcctggga cactggaacg atatcggcac 18120

cagcaacatg agcggtggcg ccttcagttg gggctctctg tggagcggca ttaaaagtat 18180

cgggtctgcc gttaaaaatt acggctcccg ggcctggaac agcagcacgg gccagatgtt 18240

gagagacaag ttgaaagagc agaacttcca gcagaaggtg gtggagggcc tggcctccgg 18300

catcaacggg gtggtggacc tggccaacca ggccgtgcag aataaaatca acagcagact 18360

ggacccccgg ccgccggtgg aggaggtgcc gccggcgctg gagacggtgt cccccgatgg 18420

gcgtggcgag aagcgcccgc ggcccgatag ggaagagacc actctggtca cgcagaccga 18480

tgagccgccc ccgtatgagg aggccctaaa gcaaggtctg cccaccacgc ggcccatcgc 18540

gcccatggcc accggggtgg tgggccgcca cacccccgcc acgctggact tgcctccgcc 18600

cgccgatgtg ccgcagcagc agaaggcggc acagccgggc ccgcccgcga ccgcctcccg 18660

ttcctccgcc ggtcctctgc gccgcgcggc cagcggcccc cgcggggggg tcgcgaggca 18720

cggcaactgg cagagcacgc tgaacagcat cgtgggtctg ggggtgcggt ccgtgaagcg 18780

ccgccgatgc tactgaatag cttagctaac gtgttgtatg tgtgtatgcg ccctatgtcg 18840

ccgccagagg agctgctgag tcgccgccgt tcgcgcgccc accaccaccg ccactccgcc 18900

cctcaagatg gcgaccccat cgatgatgcc gcagtggtcg tacatgcaca tctcgggcca 18960

ggacgcctcg gagtacctga gccccgggct ggtgcagttc gcccgcgcca ccgagagcta 19020

cttcagcctg agtaacaagt ttaggaaccc cacggtggcg cccacgcacg atgtgaccac 19080

cgaccggtct cagcgcctga cgctgcggtt cattcccgtg gaccgcgagg acaccgcgta 19140

ctcgtacaag gcgcggttca ccctggccgt gggcgacaac cgcgtgctgg acatggcctc 19200

cacctacttt gacatccgcg gggtgctgga ccggggtccc actttcaagc cctactctgg 19260

caccgcctac aactccctgg cccccaaggg cgctcccaac tcctgcgagt gggagcaaga 19320

ggaaactcag gcagttgaag aagcagcaga agaggaagaa gaagatgctg acggtcaagc 19380

tgaggaagag caagcagcta ccaaaaagac tcatgtatat gctcaggctc ccctttctgg 19440

cgaaaaaatt agtaaagatg gtctgcaaat aggaacggac gctacagcta cagaacaaaa 19500

acctatttat gcagacccta cattccagcc cgaaccccaa atcggggagt cccagtggaa 19560

tgaggcagat gctacagtcg ccggcggtag agtgctaaag aaatctactc ccatgaaacc 19620

atgctatggt tcctatgcaa gacccacaaa tgctaatgga ggtcagggtg tactaacggc 19680

aaatgcccag ggacagctag aatctcaggt tgaaatgcaa ttcttttcaa cttctgaaaa 19740

cgcccgtaac gaggctaaca acattcagcc caaattggtg ctgtatagtg aggatgtgca 19800

catggagacc ccggatacgc acctttctta caagcccgca aaaagcgatg acaattcaaa 19860

aatcatgctg ggtcagcagt ccatgcccaa cagacctaat tacatcggct tcagagacaa 19920

ctttatcggc ctcatgtatt acaatagcac tggcaacatg ggagtgcttg caggtcaggc 19980

ctctcagttg aatgcagtgg tggacttgca agacagaaac acagaactgt cctaccagct 20040

cttgcttgat tccatgggtg acagaaccag atacttttcc atgtggaatc aggcagtgga 20100

cagttatgac ccagatgtta gaattattga aaatcatgga actgaagacg agctccccaa 20160

ctattgtttc cctctgggtg gcataggggt aactgacact taccaggctg ttaaaaccaa 20220

caatggcaat aacgggggcc aggtgacttg gacaaaagat gaaacttttg cagatcgcaa 20280

tgaaataggg gtgggaaaca atttcgctat ggagatcaac ctcagtgcca acctgtggag 20340

aaacttcctg tactccaacg tggcgctgta cctaccagac aagcttaagt acaacccctc 20400

caatgtggac atctctgaca accccaacac ctacgattac atgaacaagc gagtggtggc 20460

cccggggctg gtggactgct acatcaacct gggcgcgcgc tggtcgctgg actacatgga 20520

caacgtcaac cccttcaacc accaccgcaa tgcgggcctg cgctaccgct ccatgctcct 20580

gggcaacggg cgctacgtgc ccttccacat ccaggtgccc cagaagttct ttgccatcaa 20640

gaacctcctc ctcctgccgg gctcctacac ctacgagtgg aacttcagga aggatgtcaa 20700

catggtcctc cagagctctc tgggtaacga tctcagggtg gacggggcca gcatcaagtt 20760

cgagagcatc tgcctctacg ccaccttctt ccccatggcc cacaacacgg cctccacgct 20820

cgaggccatg ctcaggaacg acaccaacga ccagtccttc aatgactacc tttccgccgc 20880

caacatgctc taccccatac ccgccaacgc caccaacgtc cccatctcca tcccctcgcg 20940

caactgggcg gccttccgcg gctgggcctt cacccgcctc aagaccaagg agaccccctc 21000

cctgggctcg ggattcgacc cctactacac ctactcgggc tctattccct acctggacgg 21060

caccttctac ctcaaccaca ctttcaagaa ggtctcggtc accttcgact cctcggtcag 21120

ctggccgggc aacgaccgtc tgctcacccc caacgagttc gagatcaagc gctcggtcga 21180

cggggaaggc tacaacgtgg cccagtgcaa catgaccaag gactggttcc tggtccagat 21240

gctggccaac tacaacatcg gctaccaggg cttctacatc ccagagagct acaaggacag 21300

gatgtactcc ttcttcagga acttccagcc catgagccgg caggtggtgg accagaccaa 21360

gtacaaggac taccaggagg tgggcatcat ccaccagcac aacaactcgg gcttcgtggg 21420

ctacctcgcc cccaccatgc gcgagggaca ggcctacccc gccaacttcc cctacccgct 21480

cataggcaag accgcggtcg acagcatcac ccagaaaaag ttcctctgcg accgcaccct 21540

ctggcgcatc cccttctcca gcaacttcat gtccatgggt gcgctctcgg acctgggcca 21600

gaacttgctc tacgccaact ccgcccacgc cctcgacatg accttcgagg tcgaccccat 21660

ggacgagccc acccttctct atgttctgtt cgaagtcttt gacgtggtcc gggtccacca 21720

gccgcaccgc ggcgtcatcg agaccgtgta cctgcgtacg cccttctcgg ccggcaacgc 21780

caccacctaa agaagcaagc cgcagtcatc gccgcctgca tgccgtcggg ttccaccgag 21840

caagagctca gggccatcgt cagagacctg ggatgcgggc cctatttttt gggcaccttc 21900

gacaagcgct tccctggctt tgtctcccca cacaagctgg cctgcgccat cgtcaacacg 21960

gccggccgcg agaccggggg cgtgcactgg ctggcctttg cctggaaccc gcgctccaaa 22020

acatgcttcc tctttgaccc cttcggcttt tcggaccagc ggctcaagca aatctacgag 22080

ttcgagtacg agggcttgct gcgtcgcagc gccatcgcct cctcgcccga ccgctgcgtc 22140

accctcgaaa agtccaccca gaccgtgcag gggcccgact cggccgcctg cggtctcttc 22200

tgctgcatgt ttctgcacgc ctttgtgcac tggcctcaga gtcccatgga ccgcaacccc 22260

accatgaact tgctgacggg ggtgcccaac tccatgctcc aaagccccca ggtcgagccc 22320

accctgcgcc gcaaccagga gcagctctac agcttcctgg agcgccactc gccctacttc 22380

cgccgccaca gcgcacagat caggagggcc acctccttct gccacttgca agagatgcaa 22440

gaagggtaat aacgatgtac acactttttt ctcaataaat ggcatttttt ttttatttat 22500

acaagctctc tggggtattc atttcccacc accaccaccc gccgttgtcg ccatctggct 22560

ctatttagaa atcgaaaggg ttctgccggg agtcgccgtg cgccacgggc agggacacgt 22620

tgcgatactg gtagcgggtg ccccacttga actcgggcac caccaggcga ggcagctcgg 22680

ggaagttttc gctccacagg ctgcgggtca gcaccagcgc gttcatcagg tcgggcgccg 22740

agatcttgaa gtcgcagttg gggccgccgc cctgcgcgcg cgagttgcgg tacaccgggt 22800

tgcagcactg gaacaccaac agcgccgggt gcttcacgct ggccagcacg ctgcggtcgg 22860

agatcagctc ggcgtccagg tcctccgcgt tgctcagcgc gaacggggtc atcttgggca 22920

cttgccgccc caggaagggc gcgtgccccg gtttcgagtt gcagtcgcag cgcagcggga 22980

tcagcaggtg cccgtgcccg gactcggcgt tggggtacag cgcgcgcatg aaggcctgca 23040

tctggcggaa ggccatctgg gccttggcgc cctccgagaa gaacatgccg caggacttgc 23100

ccgagaactg gtttgcgggg cagctggcgt cgtgcaggca gcagcgcgcg tcggtgttgg 23160

cgatctgcac cacgttgcgc ccccaccggt tcttcacgat cttggccttg gacgattgct 23220

ccttcagcgc gcgctgcccg ttctcgctgg tcacatccat ctcgatcaca tgttccttgt 23280

tcaccatgct gctgccgtgc agacacttca gctcgccctc cgtctcggtg cagcggtgct 23340

gccacagcgc gcagcccgtg ggctcgaaag acttgtaggt cacctccgcg aaggactgca 23400

ggtacccctg caaaaagcgg cccatcatgg tcacgaaggt cttgttgctg ctgaaggtca 23460

gctgcagccc gcggtgctcc tcgttcagcc aggtcttgca cacggccgcc agcgcctcca 23520

cctggtcggg cagcatcttg aagttcacct tcagctcatt ctccacgtgg tacttgtcca 23580

tcagcgtgcg cgccgcctcc atgcccttct cccaggccga caccagcggc aggctcacgg 23640

ggttcttcac catcaccgtg gccgccgcct ccgccgcgct ttcgctttcc gccccgctgt 23700

tctcttcctc ttcctcctct tcctcgccgc cgcccactcg cagcccccgc accacggggt 23760

cgtcttcctg caggcgctgc accttgcgct tgccgttgcg cccctgcttg atgcgcacgg 23820

gcgggttgct gaagcccacc atcaccagcg cggcctcttc ttgctcgtcc tcgctgtcca 23880

gaatgacctc cggggagggg gggttggtca tcctcagtac cgaggcacgc ttctttttct 23940

tcctgggggc gttcgccagc tccgcggctg cggccgctgc cgaggtcgaa ggccgagggc 24000

tgggcgtgcg cggcaccagc gcgtcttgcg agccgtcctc gtcctcctcg gactcgagac 24060

ggaggcgggc ccgcttcttc gggggcgcgc ggggcggcgg aggcggcggc ggcgacggag 24120

acggggacga gacatcgtcc agggtgggtg gacggcgggc cgcgccgcgt ccgcgctcgg 24180

gggtggtttc gcgctggtcc tcttcccgac tggccatctc ccactgctcc ttctcctata 24240

ggcagaaaga gatcatggag tctctcatgc gagtcgagaa ggaggaggac agcctaaccg 24300

ccccctctga gccctccacc accgccgcca ccaccgccaa tgccgccgcg gacgacgcgc 24360

ccaccgagac caccgccagt accaccctcc ccagcgacgc acccccgctc gagaatgaag 24420

tgctgatcga gcaggacccg ggttttgtga gcggagagga ggatgaggtg gatgagaagg 24480

agaaggagga ggtcgccgcc tcagtgccaa aagaggataa aaagcaagac caggacgacg 24540

cagataagga tgagacagca gtcgggcggg ggaacggaag ccatgatgct gatgacggct 24600

acctagacgt gggagacgac gtgctgctta agcacctgca ccgccagtgc gtcatcgtct 24660

gcgacgcgct gcaggagcgc tgcgaagtgc ccctggacgt ggcggaggtc agccgcgcct 24720

acgagcggca cctcttcgcg ccgcacgtgc cccccaagcg ccgggagaac ggcacctgcg 24780

agcccaaccc gcgtctcaac ttctacccgg tcttcgcggt acccgaggtg ctggccacct 24840

accacatctt cttccaaaac tgcaagatcc ccctctcctg ccgcgctaac cgcacccgcg 24900

ccgacaaaac cctgaccctg cggcagggcg cccacatacc tgatattgcc tctctggagg 24960

aagtgcccaa gatcttcgag ggtctcggtc gcgacgagaa acgggcggcg aacgctctgc 25020

acggagacag cgaaaacgag agtcactcgg gggtgctggt ggagctcgag ggcgacaacg 25080

cgcgcctggc cgtactcaag cgcagcatag aggtcaccca ctttgcctac ccggcgctca 25140

acctgccccc caaggtcatg agtgtggtca tgggcgagct catcatgcgc cgcgctcagc 25200

ccctggccgc ggatgcaaac ttgcaagagt cctccgagga aggcctgccc gcggtcagcg 25260

acgagcagct agcgcgctgg ctggagaccc gcgaccccgc gcagctggag gagcggcgca 25320

agctcatgat ggccgcggtg ctggtcaccg tggagctcga gtgtctgcag cgcttcttcg 25380

cggaccccga gatgcagcgc aagctcgagg agaccctgca ctacaccttc cgccagggct 25440

acgtgcgcca ggcctgcaag atctccaacg tggagctctg caacctggtc tcctacctgg 25500

gcatcctgca cgagaaccgc ctcgggcaga acgtcctgca ctccaccctc aaaggggagg 25560

cgcgccgcga ctacatccgc gactgcgcct acctcttcct ctgctacacc tggcagacgg 25620

ccatgggggt ctggcagcag tgcctggagg agcgcaacct caaggagctg gaaaagctac 25680

tcaagcgcac cctcagggac ctctggacgg gcttcaacga gcgctcggtg gccgccgcgc 25740

tggcggacat catcttcccc gagcgcctgc tcaagaccct gcagcagggc ctgcccgact 25800

tcaccagcca gagcatgctg cagaacttta ggactttcat cctggagcgc tcgggcatcc 25860

tgcctgccac ttgctgcgcg ctgcccagcg acttcgtgcc catcaagtac agggagtgcc 25920

cgccgccgct ctggggccac tgctacctct tccagctggc caactacctc gcctaccact 25980

cggacctcat ggaagacgtg agcggcgagg gcctgctcga gtgccactgc cgctgcaacc 26040

tctgcacgcc ccaccgctct ctagtctgca acccgcagct gctcagcgag agtcagatta 26100

tcggtacctt cgagctgcag ggtccctcgc ctgacgagaa gtccgcggct ccggggctga 26160

aactcactcc ggggctgtgg acttccgcct acctacgcaa atttgtacct gaggactacc 26220

acgcccacga gatcaggttc tacgaagacc aatcccgccc gcccaaggcg gagctcaccg 26280

cctgcgtcat cacccagggg cacatcctgg gccaattgca agccatcaac aaagcccgcc 26340

gagagttctt gctgaaaaag ggtcgggggg tgtacctgga cccccagtcc ggcgaggagc 26400

taaacccgct acccccgccg ccgccccagc agcgggacct tgcttcccag gatggcaccc 26460

agaaagaagc agcagccgcc gccgccgcag ccatacatgc ttctggagga agaggaggag 26520

gactgggaca gtcaggcaga ggaggtttcg gacgaggagc aggaggagat gatggaagac 26580

tgggaggagg acagcagcct agacgaggaa gcttcagagg ccgaagaggt ggcagacgca 26640

acaccatcac cctcggtcgc agccccctcg ccggggcccc tgaaatcctc cgaacccagc 26700

accagcgcta taacctccgc tcctccggcg ccggcgccac ccgcccgcag acccaaccgt 26760

agatgggaca ccacaggaac cggggtcggt aagtccaagt gcccgccgcc gccaccgcag 26820

cagcagcagc agcgccaggg ctaccgctcg tggcgcgggc acaagaacgc catagtcgcc 26880

tgcttgcaag actgcggggg caacatctct ttcgcccggc gcttcctgct attccaccac 26940

ggggtcgcct ttccccgcaa tgtcctgcat tactaccgtc atctctacag cccctactgc 27000

agcggcgacc cagaggcggc agcggcagcc acagcggcga ccaccaccta ggaagatatc 27060

ctccgcgggc aagacagcgg cagcagcggc caggagaccc gcggcagcag cggcgggagc 27120

ggtgggcgca ctgcgcctct cgcccaacga acccctctcg acccgggagc tcagacacag 27180

gatcttcccc actttgtatg ccatcttcca acagagcaga ggccaggagc aggagctgaa 27240

aataaaaaac agatctctgc gctccctcac ccgcagctgt ctgtatcaca aaagcgaaga 27300

tcagcttcgg cgcacgctgg aggacgcgga ggcactcttc agcaaatact gcgcgctcac 27360

tcttaaagac tagctccgcg cccttctcga atttaggcgg gagaaaacta cgtcatcgcc 27420

ggccgccgcc cagcccgccc agccgagatg agcaaagaga ttcccacgcc atacatgtgg 27480

agctaccagc cgcagatggg actcgcggcg ggagcggccc aggactactc cacccgcatg 27540

aactacatga gcgcgggacc ccacatgatc tcacaggtca acgggatccg cgcccagcga 27600

aaccaaatac tgctggaaca ggcggccatc accgccacgc cccgccataa tctcaacccc 27660

cgaaattggc ccgccgccct cgtgtaccag gaaaccccct ccgccaccac cgtactactt 27720

ccgcgtgacg cccaggccga agtccagatg actaactcag gggcgcagct cgcgggcggc 27780

tttcgtcacg gggcgcggcc gctccgacca ggtataagac acctgatgat cagaggccga 27840

ggtatccagc tcaacgacga gtcggtgagc tcttcgctcg gtctccgtcc ggacggaact 27900

ttccagctcg ccggatccgg ccgctcttcg ttcacgcccc gccaggcgta cctgactctg 27960

cagacctcgt cctcggagcc ccgctccgga ggcatcggaa ccctccagtt cgtggaggag 28020

ttcgtgccct cggtctactt caaccccttc tcgggacctc ccggacgcta ccccgaccag 28080

ttcattccga actttgacgc ggtgaaggac tcggcggacg gctacgactg aatgtcaggt 28140

gccgaggcag agcagcttcg cctgagacac ctcgagcact gccgccgcca caagtgcttc 28200

gcccgcggtt ccggtgagtt ctgctacttt cagctacccg aggagcatac cgaggggccg 28260

gcgcacggcg tccgcctgac cacccagggc gaggttacct gttccctcat ccgggagttc 28320

accctccgtc ccctgctagt ggagcgggag cggggtccct gtgtcctaac tatcgcctgc 28380

aactgcccta accctggatt acatcaagat ctttgctgtc atctctgtgc tgagtttaat 28440

aaacgctgag atcagaatct actggggctc ctgtcgccat cctgtgaacg ccaccgtctt 28500

cacccacccc gaccaggccc aggcgaacct cacctgcggt ctgcatcgga gggccaagaa 28560

gtacctcacc tggtacttca acggcacccc ctttgtggtt tacaacagct tcgacgggga 28620

cggagtctcc ctgaaagacc agctctccgg tctcagctac tccatccaca agaacaccac 28680

cctccaactc ttccctccct acctgccggg aacctacgag tgcgtcaccg gccgctgcac 28740

ccacctcacc cgcctgatcg taaaccagag ctttccggga acagataact ccctcttccc 28800

cagaacagga ggtgagctca ggaaactccc cggggaccag ggcggagacg taccttcgac 28860

ccttgtgggg ttaggatttt ttattaccgg gttgctggct cttttaatca aagcttcctt 28920

gagatttgtt ctttccttct acgtgtggtt aaccccttaa ttaaggggat tccatatggt 28980

acgtacattt agtccccttt aactaatcaa acactggaat caataaaaag aatcacttac 29040

ttaaaatcag acagcaggtc tctgtccagt ttattcagca gcacctcctt cccctcctcc 29100

caactctggt actccaaacg ccttctggcg gcaaacttcc tccacaccct gaagggaatg 29160

tcagattctt gctcctgtcc ctccgcaccc actatcttca tgttgttgca gatgaagcgc 29220

accaaaacgt ctgacgagag cttcaacccc gtgtacccct atgacacgga aagcggccct 29280

ccctccgtcc ctttcctcac ccctcccttc gtgtctcccg atggattcca agaaagcccc 29340

cccggggtcc tgtctctgaa cctggccgag cccctggtca cttcccacgg catgctcgcc 29400

ctgaaaatgg gaagtggcct ctccctggac gacgctggca acctcacctc tcaagatatc 29460

accaccgcta gccctcccct caaaaaaacc aagaccaacc tcagcctaga aacctcatcc 29520

cccctaactg taagcacctc aggcgccctc accgtagcag ccgccgctcc cctggcagtg 29580

gccggcacct ccctcaccat gcaatcagag gcccccctga cagtacagga tgcaaaactc 29640

accctggcca ccaaaggccc cctgaccgtg tctgaaggca aactggcctt gcaaacatcg 29700

gccccgctga cggccgctga cagcagcacc ctcaccgtta gcgccacacc accaattaat 29760

gtaagcagtg gaagtttagg cttagacatg gaagacccta tgtatactca cgatggaaaa 29820

ctgggaataa gaattggggg tccactaaga gtagtagaca gcttgcacac actcactgta 29880

gttaccggaa atggactaac tgtagataac aatgccctcc aaactagagt tacgggcgcc 29940

ctaggttatg acacatcagg aaatctacaa ttgagagctg caggaggtat gcgaattgat 30000

gcaaatggcc aacttatcct taatgtggca tacccatttg atgctcagaa caatctcagc 30060

cttagacttg gtcagggacc cctgtatata aacacagacc acaacctgga tttgaattgc 30120

aacagaggtc taaccacaac taccaccaac aacacaaaaa aacttgagac taaaattagc 30180

tcaggcttag actatgacac caatggtgct gtcattatta aacttggcac tggtctaagc 30240

ttcgacaaca caggcgccct aactgtggga aacactggtg atgataaact gactctgtgg 30300

acgaccccag acccatctcc aaattgcaga attcactcag acaaagactg caagtttact 30360

ctagtcctaa ctaagtgtgg aagccaaatc ctggcctctg tcgccgccct agcggtatca 30420

ggaaatctgg cttcgataac aggcaccgtt gccagcgtta ccatctttct cagatttgat 30480

cagaatggag tgcttatgga aaactcctcg ctagacaggc agtactggaa cttcagaaat 30540

ggcaactcaa ctaacgctgc cccctacacc aatgcagttg ggttcatgcc aaacctcgca 30600

gcatacccca aaacgcaaag ccagactgct aaaaacaaca ttgtaagtca ggtttacttg 30660

aatggagaca aatccaaacc catgaccctt accatcaccc tcaatggaac taatgaatcc 30720

agtgaaacta gccaggtgag tcactactcc atgtcattta catgggcttg ggaaagtggg 30780

caatatgcca ctgaaacctt tgccaccaac tccttcacct tttcttacat tgctgaacaa 30840

taaaaagcat gacactgatg ttcatttctg attcttattt tattattttc aaacacaaca 30900

aaatcattca agtcattctt ccatcttagc ttaatagaca cagtagctta atagacccag 30960

tagtgcaaag ccccattcta gcttataact agtggagaag tactcgccta ttatctggaa 31020

gaagcagaag agcggcggtg ggaatcatag tccgcgaacg ggatcggccg gtggtgtcgc 31080

atcaggcccc gcagcagtcg ctgccgccgc cgctccgtca agctgctgct cagggggtcc 31140

gggtccaggg actccctcag catgatgccc acggccctca gcatcagtcg tctggtgcgg 31200

cgggcgcagc agcgcatgcg gatctcgctc aggtcgctgc agtacgtgca acacagaacc 31260

accaggttgt tcaacagtcc atagttcaac acgctccagc cgaaactcat cgcgggaagg 31320

atgctaccca cgtggccgtc gtaccagatc ctcaggtaaa tcaagtggtg ccccctccag 31380

aacacgctgc ccacgtacat gatctccttg ggcatgtggc ggttcaccac ctcccggtac 31440

cacatcaccc tctggttgaa catgcagccc cggatgatcc tgcggaacca cagggccagc 31500

accgccccgc ccgccatgca gcgaagagac cccgggtccc ggcaatggca atggaggacc 31560

caccgctcgt acccgtggat catctgggag ctgaacaagt ctatgttggc acagcacagg 31620

catatgctca tgcatctctt cagcactctc aactcctcgg gggtcaaaac catatcccag 31680

ggcacgggga actcttgcag gacagcgaac cccgcagaac agggcaatcc tcgcacagaa 31740

cttacattgt gcatggacag ggtatcgcaa tcaggcagca ccgggtgatc ctccaccaga 31800

gaagcgcggg tctcggtctc ctcacagcgt ggtaaggggg ccggccgata cgggtgatgg 31860

cgggacgcgg ctgatcgtgt tcgcgaccgt gtcatgatgc agttgctttc ggacatattt 31920

cctgaagtct tagatctctc aacgcagcac cagcaccaac acttcgcagt gtaaaaggcc 31980

aagtgccgag agagtatata taggaataaa aagtgacgta aacgggcaaa gtccaaaaaa 32040

cgcccagaaa aaccgcacgc gaacctacgc cccgaaacga aagccaaaaa acactagaca 32100

ctcccttccg gcgtcaactt ccgctttccc acgctacgtc acttgcccca gtcaaacaaa 32160

ctacatatcc cgaacttcca agtcgccacg cccaaaacac cgcctacacc tccccgcccg 32220

ccggcccgcc cccaaacccg cctcccgccc cgcgccccgc cccgcgccgc ccatctcatt 32280

atcatattgg cttcaatcca aaataaggta tattattgat gatgatttaa atggcgcgcc 32340

gggcccgggc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct 32400

gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct 32460

gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga 32520

tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca 32580

cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata 32640

tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga 32700

gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc 32760

ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg 32820

cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc 32880

ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat 32940

cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact 33000

tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat 33060

tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga 33120

tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc 33180

ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga 33240

tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag 33300

cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc 33360

gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt 33420

ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct 33480

acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg 33540

cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg 33600

atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca 33660

tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga 33720

tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa 33780

aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga 33840

aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt 33900

taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt 33960

taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat 34020

agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct 34080

tggagcgaac gacctacacc gaactgagat acctacagcg tgagctttga gatagcgcca 34140

cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag 34200

agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc 34260

gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga 34320

aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca 34380

tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag 34440

ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg 34500

aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct 34560

ggcacgacag gtttcccgac tggattt 34587

Claims (19)

1. A method of constructing an expression vector based on chimpanzee ChAd3 adenovirus, the method comprising functionally deleting the E1, E3 and E4 regions in the sequence shown in SEQ ID No. 1.

2. The method according to claim 1, wherein the nucleotide corresponding to position 589-3252 of the sequence shown in SEQ ID NO 1 in the E1 region is deleted; the nucleotide corresponding to the positions 29125 and 32633 of the sequence shown in SEQ ID NO. 1 in the E3 region is deleted; and the E4 ORF6 expression cassette sequence of the chimpanzee adenovirus ChAd68 type whole genome sequence shown in SEQ ID NO: 2 in the E4 region corresponding to the positions 34634 and 37349 of the sequence shown in SEQ ID NO:1 was deleted and replaced.

3. The method of claim 2, wherein the E4 ORF6 expression cassette sequence is represented by SEQ ID NO 8.

4. The method according to any one of claims 1 to 3, wherein the composition will contain one or more compounds selected fromAsiSI、I-CeuI、PI-SceIThe sequence of the cleavage site of (a) is inserted into the deleted E1 region; and/or will containHpaI and/orPacThe sequence of the cleavage site of I is inserted into the deleted E3 region.

5. The method of claim 4, wherein there will be twoAsiSThe sequence of the cleavage site of I is inserted into the deleted E1 region.

6. The method according to claim 4 or 5, wherein the sequence shown in SEQ ID NO 6 is inserted into the deleted E1 region.

7. The method according to claim 4 or 5, wherein the sequence shown in SEQ ID NO 7 is inserted into the deleted E3 region.

8. The method of any one of the preceding claims, wherein an exogenous gene is inserted into one or more regions of deleted E1, E3, or E4.

9. The method according to claim 8, wherein the foreign gene is inserted into the deleted E1 region.

10. The method of claim 8 or 9, wherein the exogenous gene is EGFP.

11. The method of any one of the preceding claims, further comprising adding a linearization enzyme site and inserting into the prokaryotic replication plasmid.

12. The method of claim 11, wherein the linearization enzyme site isSwaIAnd (4) enzyme cutting sites.

13. The method according to claim 11 or 12, wherein the sequence of the prokaryotically replicating plasmid is as shown in SEQ ID No. 9.

14. A chimpanzee ChAd 3-based adenovirus expression vector constructed according to the method of any one of the preceding claims.

15. The expression vector of claim 14, comprising the sequence of SEQ ID NO. 11.

16. A host cell comprising the expression vector of claim 14 or 15.

17. The host cell of claim 16 which is a HEK293 cell.

18. A method for packaging and amplifying a recombinant adenovirus using the host cell of claim 16 or 17.

19. The method of claim 18, comprising the step of purifying the harvested virus stock.

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