CN113075140A - Graphene quantum dot functionalized ruthenium nano particle and preparation method and application thereof - Google Patents
Graphene quantum dot functionalized ruthenium nano particle and preparation method and application thereof Download PDFInfo
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- KJTLSVCANCCWHF-UHFFFAOYSA-N Ruthenium Chemical compound [Ru] KJTLSVCANCCWHF-UHFFFAOYSA-N 0.000 title claims abstract description 44
- 229910052707 ruthenium Inorganic materials 0.000 title claims abstract description 42
- 239000002105 nanoparticle Substances 0.000 title claims abstract description 41
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 title claims abstract description 38
- 229910021389 graphene Inorganic materials 0.000 title claims abstract description 37
- 239000002096 quantum dot Substances 0.000 title claims abstract description 37
- 238000002360 preparation method Methods 0.000 title claims abstract description 11
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims abstract description 27
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 claims abstract description 22
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 claims abstract description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 12
- 238000003756 stirring Methods 0.000 claims abstract description 10
- 238000010438 heat treatment Methods 0.000 claims abstract description 9
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 claims abstract description 7
- 238000001035 drying Methods 0.000 claims abstract description 7
- 239000012299 nitrogen atmosphere Substances 0.000 claims abstract description 6
- 238000000227 grinding Methods 0.000 claims abstract description 5
- YBCAZPLXEGKKFM-UHFFFAOYSA-K ruthenium(iii) chloride Chemical compound [Cl-].[Cl-].[Cl-].[Ru+3] YBCAZPLXEGKKFM-UHFFFAOYSA-K 0.000 claims abstract description 5
- 238000001354 calcination Methods 0.000 claims abstract description 3
- 238000000034 method Methods 0.000 claims description 15
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 12
- 239000003987 organophosphate pesticide Substances 0.000 claims description 11
- 238000001514 detection method Methods 0.000 claims description 7
- 238000001816 cooling Methods 0.000 claims description 6
- 238000009210 therapy by ultrasound Methods 0.000 claims description 4
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 239000007787 solid Substances 0.000 claims description 2
- 238000003828 vacuum filtration Methods 0.000 claims description 2
- 239000000575 pesticide Substances 0.000 abstract description 6
- 230000003197 catalytic effect Effects 0.000 abstract description 5
- 239000000243 solution Substances 0.000 description 16
- 239000000047 product Substances 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 4
- OEBRKCOSUFCWJD-UHFFFAOYSA-N dichlorvos Chemical compound COP(=O)(OC)OC=C(Cl)Cl OEBRKCOSUFCWJD-UHFFFAOYSA-N 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000002835 absorbance Methods 0.000 description 3
- 229960004373 acetylcholine Drugs 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 229950001327 dichlorvos Drugs 0.000 description 3
- 230000001404 mediated effect Effects 0.000 description 3
- 239000002082 metal nanoparticle Substances 0.000 description 3
- 102000012440 Acetylcholinesterase Human genes 0.000 description 2
- 108010022752 Acetylcholinesterase Proteins 0.000 description 2
- OIPILFWXSMYKGL-UHFFFAOYSA-N acetylcholine Chemical compound CC(=O)OCC[N+](C)(C)C OIPILFWXSMYKGL-UHFFFAOYSA-N 0.000 description 2
- 229940022698 acetylcholinesterase Drugs 0.000 description 2
- 238000003837 high-temperature calcination Methods 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 229910021645 metal ion Inorganic materials 0.000 description 2
- 239000004570 mortar (masonry) Substances 0.000 description 2
- 239000002086 nanomaterial Substances 0.000 description 2
- 230000001590 oxidative effect Effects 0.000 description 2
- 238000001878 scanning electron micrograph Methods 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- UAIUNKRWKOVEES-UHFFFAOYSA-N 3,3',5,5'-tetramethylbenzidine Chemical compound CC1=C(N)C(C)=CC(C=2C=C(C)C(N)=C(C)C=2)=C1 UAIUNKRWKOVEES-UHFFFAOYSA-N 0.000 description 1
- 102000004316 Oxidoreductases Human genes 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- 108010009736 Protein Hydrolysates Proteins 0.000 description 1
- 238000002441 X-ray diffraction Methods 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000000862 absorption spectrum Methods 0.000 description 1
- -1 acetylcholine ester Chemical class 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000003013 cytotoxicity Effects 0.000 description 1
- 231100000135 cytotoxicity Toxicity 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000002290 gas chromatography-mass spectrometry Methods 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 125000002883 imidazolyl group Chemical group 0.000 description 1
- 238000004811 liquid chromatography Methods 0.000 description 1
- 238000004895 liquid chromatography mass spectrometry Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 239000000447 pesticide residue Substances 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 239000013076 target substance Substances 0.000 description 1
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
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- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/33—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using ultraviolet light
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Abstract
The invention discloses a preparation method of graphene quantum dot functionalized ruthenium nanoparticles, which comprises the following steps: (1) dissolving citric acid and histidine in water, uniformly stirring, and heating at 170-190 ℃ for 2-3 hours to obtain histidine functionalized graphene quantum dots; (2) dispersing the histidine functionalized graphene quantum dots obtained in the step (1) in water, and adjusting the pH of the solution to 6-8; then adding a ruthenium trichloride solution into the solution under the condition of continuously stirring, standing, collecting a product, and drying; (3) and (3) grinding the dried product obtained in the step (2), and calcining at 300-600 ℃ in a nitrogen atmosphere to obtain the graphene quantum dot functionalized ruthenium nano particle. The graphene quantum dot functionalized ruthenium nano particle has high catalytic activity and can be used for detecting organophosphorus pesticides.
Description
Technical Field
The invention relates to the technical field of biosensing, in particular to graphene quantum dot functionalized ruthenium nanoparticles and a preparation method and application thereof.
Background
Organophosphorus Pesticides (OPs) are widely used worldwide to increase agricultural productivity, but overuse of pesticides can cause ecosystem pollution and pose a great potential threat to human health. Therefore, the establishment of an ultra-sensitive and accurate method for rapidly screening the pesticide residues is of great significance.
At present, the detection means of organophosphorus pesticides mainly comprises liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), liquid chromatography-mass spectrometry (LC-MS) and the like. Although these methods have high sensitivity, high cost, complicated operation, and are not suitable for rapid detection.
The metal nanoparticle-mediated analysis method utilizes the strong oxidizing property and the catalytic property of the metal nanoparticles to realize signal conversion so as to analyze a target substance. The metal nanoparticle-mediated analysis method has the advantages of rapid detection, simple operation and high sensitivity, and becomes one of the research hotspots in the analysis field in recent years.
Disclosure of Invention
The technical problem to be solved by the invention is to provide a preparation method of graphene quantum dot functionalized ruthenium nanoparticles, and the graphene quantum dot functionalized ruthenium nanoparticles prepared by the method have good catalytic activity, can be used for detecting organophosphorus pesticide dichlorvos, and have a detection limit as low as 3 ng/ml.
In order to solve the technical problems, the invention provides the following technical scheme:
the invention provides a preparation method of graphene quantum dot functionalized ruthenium nanoparticles, which comprises the following steps:
(1) dissolving citric acid and histidine in water, uniformly stirring, and heating at 170-190 ℃ for 2-3 hours to obtain histidine functionalized graphene quantum dots;
(2) dispersing the histidine functionalized graphene quantum dots in water, and adjusting the pH of a solution to 6-8; then adding a ruthenium trichloride solution into the solution under the condition of continuously stirring, standing, collecting a product, and drying;
(3) grinding the dried product obtained in the step (2), and then carrying out nitrogen atmosphere at 1-10 ℃ for min-1The temperature rise rate is programmed to be 300-600 ℃, then the mixture is calcined for 1-10 hours, and the graphene quantum dot functionalized ruthenium nano particle is obtained after cooling.
In the step (1), histidine is adopted to modify the graphene quantum dots, and the obtained histidine-functionalized graphene quantum dots contain imidazole groups, so that ruthenium metal ions can be subsequently complexed.
Further, in the step (1), the ratio of citric acid to histidine is 0.5-1.5: 1, preferably 1: 1.
Further, in the step (1), the heating temperature is 180 ℃, and the heating time is 2.5 h.
Further, in the step (2), the dispersing method of the histidine-functionalized graphene quantum dots comprises the following steps: adding the histidine functionalized graphene quantum dots into water, and carrying out ultrasonic treatment and stirring for 30-90 min, so that the histidine functionalized graphene quantum dots are dispersed in the water.
Further, in the step (2), the pH of the solution is adjusted to 7 by using a sodium hydroxide solution or a potassium hydroxide solution.
Further, in the step (2), after standing, the solution is subjected to vacuum filtration, and then the solid is collected and dried.
Further, in the step (2), the drying temperature is 55-75 ℃, and the drying time is 6-10 h.
In the step (3), the dried product is calcined at 300-600 ℃ in a nitrogen atmosphere, so that the ruthenium metal ions are reduced into nanoparticles. Further, the temperature of the calcination was 500 ℃.
The invention also provides a graphene quantum dot functionalized ruthenium nano particle prepared by the method.
The third aspect of the invention provides an application of the graphene quantum dot functionalized ruthenium nano particle in organophosphorus pesticide detection.
The invention utilizes the inhibition effect of organophosphorus pesticide on acetylcholinesterase activity to establish the quantitative analysis of organophosphorus pesticide by ruthenium-mediated spectrophotometry. The principle is as follows: based on the inhibition effect of the organophosphorus pesticide on acetylcholinesterase and the reaction of oxidizing 3,3 ', 5, 5' -Tetramethylbenzidine (TMB) by ruthenium nanoparticles, a simple and sensitive colorimetric analysis method for detecting the organophosphorus pesticide is established. When organophosphorus pesticide does not exist in the system, the thioacetylcholine hydrolysate, namely the thiocholine, is complexed with the ruthenium nano particles, TMB cannot be oxidized, and lower absorbance is generated; when the organophosphorus pesticide exists, the thiocholine cannot be generated, the TMB is oxidized by the ruthenium nano particles to form ox-TMB, and the absorbance is enhanced.
Compared with the prior art, the invention has the beneficial effects that:
the graphene quantum dots are a quasi-zero-dimensional nano material, and have the advantages of excellent optical stability, chemical inertness, low cytotoxicity, biocompatibility and the like. The graphene quantum dot functionalized ruthenium nanoparticle compound has high catalytic activity, can be used for detecting organophosphorus pesticide dichlorvos, has a detection limit as low as 3ng/ml, and has a wide application prospect.
Drawings
Fig. 1 is a process flow diagram for the synthesis of graphene quantum dot functionalized ruthenium nanoparticles;
fig. 2 is a scanning electron micrograph of graphene quantum dot functionalized ruthenium nanoparticles;
fig. 3 is an XRD pattern of graphene quantum dot functionalized ruthenium nanoparticles;
FIG. 4 is a graph of ultraviolet absorption spectra of DDVP pesticides detected by graphene quantum dot functionalized ruthenium nanoparticle oxidase activity.
Detailed Description
The present invention is further described below in conjunction with the following figures and specific examples so that those skilled in the art may better understand the present invention and practice it, but the examples are not intended to limit the present invention.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.
The experimental methods used in the following examples are conventional methods unless otherwise specified, and materials, reagents and the like used therein are commercially available without otherwise specified.
Example 1
The embodiment provides a graphene quantum dot functionalized ruthenium nanoparticle, and the preparation method comprises the following steps:
(1) weighing histidine and citric acid according to the ratio of 1:1, adding the histidine and citric acid into a beaker, then placing the beaker into an oven, setting the temperature of the oven at 180 ℃, heating for 2 hours until the reaction is finished, and cooling to obtain the histidine functionalized graphene quantum dot.
(2) Adding the histidine-functionalized graphene quantum dots prepared in the step (1) into water, and performing ultrasonic treatment and stirring for 30min respectively to dissolve the histidine-functionalized graphene quantum dots. Then dropwise adding a sodium hydroxide solution, and adjusting the pH value of the solution to 7; then, the prepared ruthenium trichloride solution is dripped into the solution, the solution is kept stand for half an hour, and then is filtered in vacuum for 1 hour to be completely precipitated, and is dried in vacuum at 65 ℃.
(3) Grinding the dried product obtained in step (2) with a mortar for about 2h, then placing in a crucible under nitrogen atmosphere at 5 ℃ for min-1The temperature rise rate of the process is programmed to be 500 ℃, and then the high-temperature calcination is carried out for 2 hours while the temperature is kept at 500 ℃. And naturally cooling at night to obtain the graphene quantum dot functionalized ruthenium nano particles.
Fig. 2 is an SEM image of the graphene quantum dot functionalized ruthenium nanoparticles, and it can be seen that the composite material has a uniform size and good dispersibility at about 40 nm.
Fig. 3 is an XRD spectrum of the graphene quantum dot functionalized ruthenium nanoparticle, wherein peaks 25.29 ° and 43.95 ° are peaks of graphitic carbon, and peaks at other positions are peaks of the ruthenium nanoparticle, which indicates that the graphene quantum dot functionalized ruthenium nanoparticle is successfully prepared by the present invention.
The ultraviolet absorption spectrogram shown in the figure 4 is obtained by preparing dichlorvos pesticides with different concentrations, adding acetylcholine ester with a certain concentration, incubating in HAc-NaAc buffer solution for 30min, adding acetylcholine to react with the acetylcholine for 60min, and adding a solvent to a catalytic system of a ruthenium nano material and TMB. As can be seen from FIG. 4, at a wavelength of 652nm, when an organophosphate pesticide is present, thiocholine is not produced, and the ruthenium nanoparticles oxidize TMB to form ox-TMB, resulting in an increase in absorbance.
Example 2
The embodiment provides a graphene quantum dot functionalized ruthenium nanoparticle, and the preparation method comprises the following steps:
(1) weighing histidine and citric acid according to the ratio of 1:1, adding the histidine and citric acid into a beaker, then placing the beaker into an oven, setting the temperature of the oven at 180 ℃, heating for 2 hours until the reaction is finished, and cooling to obtain the histidine functionalized graphene quantum dot.
(2) Adding the histidine-functionalized graphene quantum dots prepared in the step (1) into water, and performing ultrasonic treatment and stirring for 30min respectively to dissolve the histidine-functionalized graphene quantum dots. Then dropwise adding a sodium hydroxide solution, and adjusting the pH value of the solution to 8; then, the prepared ruthenium trichloride solution is dripped into the solution, the solution is kept stand for half an hour, and then is filtered in vacuum for 1 hour to be completely precipitated, and is dried in vacuum at the temperature of 60 ℃.
(3) Grinding the dried product obtained in step (2) with a mortar for about 2h, then placing in a crucible under nitrogen atmosphere at 5 ℃ for min-1The temperature rise rate of the catalyst is programmed to be 600 ℃, and then the high-temperature calcination is carried out for 2 hours while the temperature is kept at 600 ℃. And naturally cooling at night to obtain the graphene quantum dot functionalized ruthenium nano particles.
The above-mentioned embodiments are merely preferred embodiments for fully illustrating the present invention, and the scope of the present invention is not limited thereto. The equivalent substitution or change made by the technical personnel in the technical field on the basis of the invention is all within the protection scope of the invention. The protection scope of the invention is subject to the claims.
Claims (10)
1. A preparation method of graphene quantum dot functionalized ruthenium nanoparticles is characterized by comprising the following steps:
(1) dissolving citric acid and histidine in water, uniformly stirring, and heating at 170-190 ℃ for 2-3 hours to obtain histidine functionalized graphene quantum dots;
(2) dispersing the histidine functionalized graphene quantum dots in water, and adjusting the pH of a solution to 6-8; then adding a ruthenium trichloride solution into the solution under the condition of continuously stirring, standing, collecting a product, and drying;
(3) grinding the dried product obtained in the step (2), and then carrying out nitrogen atmosphere at 1-10 ℃ for min-1The temperature rise rate is programmed to be 300-600 ℃, then the mixture is calcined for 1-10 hours, and the graphene quantum dot functionalized ruthenium nano particle is obtained after cooling.
2. The preparation method of the graphene quantum dot functionalized ruthenium nanoparticle according to claim 1, wherein in the step (1), the mass ratio of citric acid to histidine is 0.5-1.5: 1.
3. The method for preparing the graphene quantum dot functionalized ruthenium nanoparticle according to claim 1, wherein in the step (1), the heating temperature is 180 ℃ and the heating time is 2.5 h.
4. The method for preparing graphene quantum dot functionalized ruthenium nanoparticles according to claim 1, wherein in the step (2), the dispersing method of the histidine-functionalized graphene quantum dots comprises: adding the histidine functionalized graphene quantum dots into water, and performing ultrasonic treatment and stirring for 30-90 min to disperse the histidine functionalized graphene quantum dots into the water.
5. The method for preparing graphene quantum dot functionalized ruthenium nanoparticles according to claim 1, wherein in the step (2), a sodium hydroxide solution or a potassium hydroxide solution is used for adjusting the pH value of the solution to 7.
6. The method for preparing the graphene quantum dot functionalized ruthenium nano particle according to claim 1, wherein in the step (2), after standing, the solution is subjected to vacuum filtration, and then the solid is collected and dried.
7. The preparation method of the graphene quantum dot functionalized ruthenium nanoparticle according to claim 1, wherein in the step (2), the drying temperature is 55-75 ℃, and the drying time is 6-10 h.
8. The method for preparing the graphene quantum dot functionalized ruthenium nanoparticle according to claim 1, wherein the calcining temperature in the step (3) is 500 ℃.
9. The graphene quantum dot functionalized ruthenium nanoparticle prepared according to the method of any one of claims 1 to 8.
10. The graphene quantum dot functionalized ruthenium nanoparticle of claim 9 for use in organophosphorus pesticide detection.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114272862A (en) * | 2021-12-23 | 2022-04-05 | 江苏省特种设备安全监督检验研究院 | Ruthenium-based metal ion liquid polymer microsphere artificial enzyme and preparation method and application thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109323896A (en) * | 2018-09-12 | 2019-02-12 | 东莞理工学院 | Pesticide detection method based on graphene quantum dot |
| CN110567924A (en) * | 2019-09-02 | 2019-12-13 | 江南大学 | Preparation method of graphene-rare earth composite material and application of graphene-rare earth composite material in benzimidazole pesticide residue combined toxicity effect |
| CN110577213A (en) * | 2019-09-11 | 2019-12-17 | 江南大学 | Dual-functionalized graphene quantum dot and preparation method and application thereof |
| CN110632142A (en) * | 2019-08-28 | 2019-12-31 | 江南大学 | Preparation method and application of electrochemical biosensor based on gold palladium-graphene quantum dot composite material |
| CN111607388A (en) * | 2020-06-23 | 2020-09-01 | 江苏省特种设备安全监督检验研究院 | Preparation method and application of graphene quantum dot-rare earth up-conversion compound |
| CN111696790A (en) * | 2020-06-23 | 2020-09-22 | 江苏省特种设备安全监督检验研究院 | Preparation method of high-dispersion graphene-ruthenium oxide nanocomposite |
-
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- 2021-03-29 CN CN202110335208.9A patent/CN113075140A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109323896A (en) * | 2018-09-12 | 2019-02-12 | 东莞理工学院 | Pesticide detection method based on graphene quantum dot |
| CN110632142A (en) * | 2019-08-28 | 2019-12-31 | 江南大学 | Preparation method and application of electrochemical biosensor based on gold palladium-graphene quantum dot composite material |
| CN110567924A (en) * | 2019-09-02 | 2019-12-13 | 江南大学 | Preparation method of graphene-rare earth composite material and application of graphene-rare earth composite material in benzimidazole pesticide residue combined toxicity effect |
| CN110577213A (en) * | 2019-09-11 | 2019-12-17 | 江南大学 | Dual-functionalized graphene quantum dot and preparation method and application thereof |
| CN111607388A (en) * | 2020-06-23 | 2020-09-01 | 江苏省特种设备安全监督检验研究院 | Preparation method and application of graphene quantum dot-rare earth up-conversion compound |
| CN111696790A (en) * | 2020-06-23 | 2020-09-22 | 江苏省特种设备安全监督检验研究院 | Preparation method of high-dispersion graphene-ruthenium oxide nanocomposite |
Non-Patent Citations (2)
| Title |
|---|
| LI RUIYI等: "Histidine-functionalized graphene quantum dot-graphene micro-aerogel based voltammetric sensing of dopamine", 《SENSORS AND ACTUATORS B: CHEMICAL》 * |
| 丁卓峰等: "组氨酸功能化碳点/石墨烯气凝胶的制备及超级电容器性能", 《无机材料学报》 * |
Cited By (2)
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| CN114272862A (en) * | 2021-12-23 | 2022-04-05 | 江苏省特种设备安全监督检验研究院 | Ruthenium-based metal ion liquid polymer microsphere artificial enzyme and preparation method and application thereof |
| CN114272862B (en) * | 2021-12-23 | 2022-09-09 | 江苏省特种设备安全监督检验研究院 | Ruthenium-based metal ion liquid polymer microsphere artificial enzyme and preparation method and application thereof |
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