CN113068779B - Preparation method of natural anti-browning NFC apple juice - Google Patents

Preparation method of natural anti-browning NFC apple juice Download PDF

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CN113068779B
CN113068779B CN202110380623.6A CN202110380623A CN113068779B CN 113068779 B CN113068779 B CN 113068779B CN 202110380623 A CN202110380623 A CN 202110380623A CN 113068779 B CN113068779 B CN 113068779B
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juice
seabuckthorn
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apples
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CN113068779A (en
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周磊
刘伟
陈恢文
禹凯博
邹立强
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Nanchang University
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/02Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation containing fruit or vegetable juices
    • A23L2/04Extraction of juices
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/42Preservation of non-alcoholic beverages
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/42Preservation of non-alcoholic beverages
    • A23L2/44Preservation of non-alcoholic beverages by adding preservatives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/70Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
    • A23L2/72Clarifying or fining of non-alcoholic beverages; Removing unwanted matter by filtration
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/40Colouring or decolouring of foods
    • A23L5/41Retaining or modifying natural colour by use of additives, e.g. optical brighteners
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Life Sciences & Earth Sciences (AREA)
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Abstract

The invention discloses a preparation method of natural anti-browning NFC apple juice, and belongs to the technical field of food processing. It comprises the following steps: (1) pretreatment: preparing and treating seabuckthorn, apples and hawthorns to obtain crude seabuckthorn extract, massive apples and massive hawthorns; (2) preparing juice: preparing the blocky apples and the blocky hawthorns pretreated in the step (1) for juicing, and filtering the squeezed turbid juice to obtain clear juice; preparing the seabuckthorn crude extract pretreated in the step (1) and an anti-browning agent, and adding the seabuckthorn crude extract and the anti-browning agent into the filtered clear juice; (3) and (3) sterilizing and filling the fruit juice: and (2) preparing the fruit juice prepared in the step (1), sending the fruit juice into a high-pressure homogenizer for homogenization treatment, then carrying out ultra-high pressure technology sterilization, then sending into an aseptic filling machine for filling, sealing by using a packaging machine, and finally carrying out spray cooling to obtain a finished product. The invention uses the purely natural hawthorn and the sea buckthorn to replace additives to improve the quality of the apple juice, thereby ensuring the absolute naturalness, greenness, safety and nutrition of the juice.

Description

Preparation method of natural anti-browning NFC apple juice
Technical Field
The invention belongs to the technical field of food processing, and particularly relates to a preparation method of natural anti-browning NFC apple juice.
Background
The apple is one of four fruits in the world, the pulp of the apple is fine and smooth, and the apple is rich in functional components such as a large amount of organic acids (such as malic acid), phenolic compounds, mineral substances and the like, and is rich in nutrition. More than 60% of the world apples are used for fresh marketing, and even less than 10% of the world apples are used for processing in China. The apple juice is the most popular and rapidly developed product in apple processing products, and has a plurality of problems in domestic processing and production. For example, apple juice is easy to brown due to the fact that the apple juice is rich in polyphenols, so that the color of the apple is changed and the sensory quality of the apple is affected; for example, NFC (non from concentrated) apple juice, which is rapidly spreading to the world, has poor stability, is prone to precipitation and delamination due to its large content.
In order to solve these problems, manufacturers of the food industry have made a lot of attempts, but a series of solutions have been proposed which use various food additives without exception, but which are contrary to the increasingly popular concept of cleaning labels. We have sought to use one or more ingredients of natural origin to remove or replace artificial or chemical additives used in food products, to simplify the ingredient list, and to preserve the natural attributes of the food product in the labeled ingredient column.
Meanwhile, the traditional heat processing method can cause damage to the nutrition and sensory quality of the product caused by high temperature, such as generation of cooking flavor and non-enzymatic browning, and the ultrahigh pressure technology is used as a brand-new non-heat processing technology.
Disclosure of Invention
1. Problems to be solved
Aiming at the problems in the prior art, the invention provides the preparation method of the natural anti-browning NFC apple juice, and pure natural hawthorn and sea buckthorn are used for replacing additives to improve the quality of the apple juice, so that the absolute naturalness, greenness, safety and nutrition of the apple juice are ensured.
2. Technical scheme
In order to solve the above problems, the present invention adopts the following technical solutions.
A preparation method of natural anti-browning NFC apple juice comprises the following steps:
(1) pretreatment: preparing and treating seabuckthorn, apples and hawthorns to obtain crude seabuckthorn extract, massive apples and massive hawthorns;
(2) preparing juice: preparing the blocky apples and the blocky hawthorns pretreated in the step (1) for juicing, and filtering the squeezed turbid juice to obtain clear juice for later use; adding the seabuckthorn crude extract pretreated in the step (1) and the anti-browning agent into the filtered clear juice to obtain fruit juice;
(3) and (3) sterilizing and filling the fruit juice: and (2) preparing the fruit juice prepared in the step (1), sending the fruit juice into a high-pressure homogenizer for homogenization treatment, then carrying out ultra-high pressure technology sterilization, then sending into an aseptic filling machine for filling, sealing by using a packaging machine, and finally carrying out spray cooling to obtain a finished product.
In the preparation method of the natural anti-browning NFC apple juice,
the processing mode of the sea buckthorn in the step (1) is as follows:
preparing raw seabuckthorn pulp;
taking out the raw juice of seabuckthorn, and adopting flowing water to thaw;
filtering the unfrozen raw seabuckthorn pulp by using a 80-mesh screen;
centrifuging the filtered seabuckthorn raw pulp;
removing impurities of the lower layer precipitate and the upper layer aggregate from the centrifuged raw seabuckthorn pulp, and taking clear liquid of the middle layer;
filtering the clear liquid in the middle layer with 120 mesh screen to obtain crude extract of fructus Hippophae.
In the preparation method of the natural anti-browning NFC apple juice,
the storage condition of the raw juice of seabuckthorn is-20 ℃;
the centrifugal force of the centrifugal treatment of the raw seabuckthorn pulp is 4800g, and the centrifugal treatment time of the raw seabuckthorn pulp is 15 min.
In the preparation method of the natural anti-browning NFC apple juice,
the apple and hawthorn processing mode in the step (1) is as follows:
preparing apples and hawthorns;
cleaning apple and hawthorn, removing core of the apple and the hawthorn, and cutting into blocks;
soaking the cut apples and the hawthorn into ascorbic acid solution for color protection.
In the preparation method of the natural anti-browning NFC apple juice,
the storage conditions of the apples and the hawthorns are-3-4 ℃;
the mass concentration of the ascorbic acid solution for color protection is 500 mg/L.
In the preparation method of the natural anti-browning NFC apple juice,
the anti-browning agent in the step (2) comprises D-tryptophan, chitosan and mannitol, wherein the mass ratio of the D-tryptophan to the chitosan to the mannitol is 2: 17: 10, wherein the addition mass of the anti-browning agent is 0.1 percent of the mass of the massive apples;
in the step (2), the adding mass of the blocky hawthorns is 4% of the mass of the blocky apples;
and (3) mixing the blocky apples and the blocky hawthorns in the step (2) and juicing, sieving the squeezed juice with a 80-mesh sieve, and then placing the sieved juice in a container for later use.
In the preparation method of the natural anti-browning NFC apple juice,
the mixing and juicing equipment is a low-speed squeezing type normal juice machine;
the adding mass of the seabuckthorn crude extract is 5% of the mass of the filtered clear liquid juice.
In the preparation method of the natural anti-browning NFC apple juice,
the pressure of the homogenization treatment in the step (3) is 40MPa, and the temperature is set to be 25 ℃.
In the preparation method of the natural anti-browning NFC apple juice,
the sterilization pressure of the ultra-high pressure technique sterilization in the step (3) is 600MPa, the sterilization time is 3min, and the sterilization temperature is below 25 ℃.
In the preparation method of the natural anti-browning NFC apple juice,
the pressure boosting process of the ultra-high pressure technology sterilization comprises two steps:
the first step is to rapidly increase the pressure to 150 MPa;
the second step is to increase the pressure to 600MPa at a constant speed within 4 min.
3. Advantageous effects
Compared with the prior art, the invention has the beneficial effects that:
according to the preparation method of the natural anti-browning NFC apple juice, the pure natural hawthorn and the sea buckthorn are used as ingredients, the deterioration property that the apple juice is easy to brown and precipitate is improved by utilizing the characteristics of high pectin content and high viscosity of the hawthorn and high organic acid and flavonoid compound content of the sea buckthorn, so that the addition of artificial or chemical additives is basically removed, the processing process is simplified to the greatest extent, and the clean label concept which is rapidly developed in the world is responded, so that a label ingredient column is cleaner and more natural, and consumers with increasingly enhanced health and safety awareness are willing to buy bills; moreover, the hawthorn and the sea buckthorn also have unique flavor and nutrition, and after the hawthorn and the sea buckthorn are added into the apple juice, the mixed juice of the hawthorn and the sea buckthorn is sour, sweet, delicious and fragrant. And the low-speed press-filtering type juicer is used for juicing, the juice yield is high, and the nutrition and the flavor are completely preserved. Meanwhile, the novel ultrahigh pressure technology is used for sterilizing the fruit juice, so that the damage to the nutrition and sensory quality of the product caused by high temperature due to the heat treatment on the apple juice is avoided, and the original qualities of the fruit juice such as nutrition, color and flavor are well maintained. In addition, by adding the anti-browning agent,
drawings
Fig. 1 is a diagram illustrating the effects of embodiments 1, 3 and 4 of the present invention.
Detailed Description
The invention is further described with reference to specific examples.
It should be noted that, in the following description,
the sea-buckthorn, the apple and the hawthorn need to be refrigerated in advance to slow down the browning degree in the storage period;
the juicing equipment adopts a low-speed juicing type juicer as far as possible so as to obtain more pure taste and flavor of the juice;
the pressure boosting of the ultra-high pressure technology sterilization is divided into two steps to ensure that the temperature of the juice is below 25 ℃ in the processing process.
A preparation method of natural anti-browning NFC apple juice comprises the following steps:
(1) pretreatment: preparing and treating seabuckthorn, apples and hawthorns to obtain crude seabuckthorn extract, massive apples and massive hawthorns;
(2) preparing juice: preparing the blocky apples and the blocky hawthorns pretreated in the step (1) for juicing, and filtering the squeezed turbid juice to obtain clear juice for later use; adding the seabuckthorn crude extract pretreated in the step (1) and the anti-browning agent into the filtered clear juice to obtain fruit juice;
(3) and (3) sterilizing and filling the fruit juice: and (2) preparing the fruit juice prepared in the step (1), sending the fruit juice into a high-pressure homogenizer for homogenization treatment, then carrying out ultra-high pressure technology sterilization, then sending into an aseptic filling machine for filling, sealing by using a packaging machine, and finally carrying out spray cooling to obtain a finished product.
In the preparation method of the natural anti-browning NFC apple juice,
the processing mode of the sea buckthorn in the step (1) is as follows:
preparing raw seabuckthorn pulp;
taking out the raw juice of seabuckthorn, and adopting flowing water to thaw;
filtering the unfrozen raw seabuckthorn pulp by using a 80-mesh screen;
centrifuging the filtered seabuckthorn raw pulp;
removing impurities of the lower layer precipitate and the upper layer aggregate from the centrifuged raw seabuckthorn pulp, and taking clear liquid of the middle layer;
filtering the clear liquid in the middle layer with 120 mesh screen to obtain crude extract of fructus Hippophae.
In the preparation method of the natural anti-browning NFC apple juice,
the storage condition of the raw juice of seabuckthorn is-20 ℃;
the centrifugal force of the centrifugal treatment of the raw seabuckthorn pulp is 4800g, and the centrifugal treatment time of the raw seabuckthorn pulp is 15 min.
In the preparation method of the natural anti-browning NFC apple juice,
the apple and hawthorn processing mode in the step (1) is as follows:
preparing apples and hawthorns;
cleaning apple and hawthorn, removing core of the apple and the hawthorn, and cutting into blocks;
soaking the cut apples and the hawthorn into ascorbic acid solution for color protection.
In the preparation method of the natural anti-browning NFC apple juice,
the storage conditions of the apples and the hawthorns are-3-4 ℃;
the mass concentration of the ascorbic acid solution for color protection is 500 mg/L.
In the preparation method of the natural anti-browning NFC apple juice,
the anti-browning agent in the step (2) comprises D-tryptophan, chitosan and mannitol, wherein the mass ratio of the D-tryptophan to the chitosan to the mannitol is 2: 17: 10, wherein the addition mass of the anti-browning agent is 0.1 percent of the mass of the massive apples;
in the step (2), the adding mass of the blocky hawthorns is 4% of the mass of the blocky apples;
and (3) mixing the blocky apples and the blocky hawthorns in the step (2) and juicing, sieving the squeezed juice with a 80-mesh sieve, and then placing the sieved juice in a container for later use.
In the preparation method of the natural anti-browning NFC apple juice,
the mixing and juicing equipment is a low-speed squeezing type normal juice machine;
the adding mass of the seabuckthorn crude extract is 5% of the mass of the filtered clear liquid juice.
In the preparation method of the natural anti-browning NFC apple juice,
the pressure of the homogenization treatment in the step (3) is 40MPa, and the temperature is set to be 25 ℃.
In the preparation method of the natural anti-browning NFC apple juice,
the sterilization pressure of the ultra-high pressure technique sterilization in the step (3) is 600MPa, the sterilization time is 3min, and the sterilization temperature is below 25 ℃.
In the preparation method of the natural anti-browning NFC apple juice,
the pressure boosting process of the ultra-high pressure technology sterilization comprises two steps:
the first step is to rapidly increase the pressure to 150 MPa;
the second step is to increase the pressure to 600MPa at a constant speed within 4 min.
Example 1
Selecting fresh fructus Mali Pumilae and fructus crataegi refrigerated at 4 deg.C, cleaning, removing core, and cutting into pieces; soaking the fragments in 500mg/L ascorbic acid for color protection; weighing 1000g of apple fragments, putting the apple fragments into a low-speed pressure filtration type juicer for juicing, screening the juice with a 80-mesh screen, and then feeding the juice into a high-pressure homogenizer for homogenization treatment under the conditions of 40MPa and 25 ℃; sterilizing the homogenized juice at 600MPa and 20 deg.C by ultra-high pressure technique; filling the sterilized fruit juice in a filling machine, and sealing with a sealing machine; and (4) spraying, cooling and cooling the filled and sealed fruit juice to obtain a finished fruit juice product.
Example 2
Selecting fresh fructus Mali Pumilae and fructus crataegi refrigerated at 4 deg.C, cleaning, removing core, and cutting into pieces; soaking the fragments in 500mg/L ascorbic acid for color protection; weighing 960g of apple fragments and 40g of hawthorn fragments, putting into a low-speed pressure filtration type juicer for juicing, and sieving the juice with a 80-mesh sieve; then sending the mixture into a high-pressure homogenizer for homogenization treatment under the conditions of 40MPa and 25 ℃; sterilizing the homogenized juice at 600MP a and 20 deg.C by ultra-high pressure technique; filling the sterilized fruit juice in a filling machine, and sealing with a sealing machine; and (4) spraying, cooling and cooling the filled and sealed fruit juice to obtain a finished fruit juice product.
Example 3
Selecting fresh fructus Mali Pumilae and fructus crataegi refrigerated at 4 deg.C, cleaning, removing core, and cutting into pieces; soaking the fragments in 500mg/L ascorbic acid for color protection; weighing 1000g of apple pieces, putting the apple pieces into a low-speed pressure filtration type juicer for juicing, sieving the juice with a 80-mesh sieve, and adding 5% of crude extract of seabuckthorn; then sending the mixture into a high-pressure homogenizer for homogenization treatment under the conditions of 40MPa and 25 ℃; sterilizing the homogenized juice at 600MPa and 20 deg.C by ultra-high pressure technique; filling the sterilized fruit juice in a filling machine, and sealing with a sealing machine; and (4) spraying, cooling and cooling the filled and sealed fruit juice to obtain a finished fruit juice product.
Example 4
Selecting fresh fructus Mali Pumilae and fructus crataegi refrigerated at 4 deg.C, cleaning, removing core, and cutting into pieces; soaking the fragments in 500mg/L ascorbic acid for color protection; weighing 960g of apple fragments and 40g of hawthorn fragments, placing into a low-speed pressure filtration type juicer for juicing, sieving the obtained juice with a 80-mesh sieve, and adding 5% of crude extract of sea buckthorn and 0.1% of anti-browning agent; then sending the mixture into a high-pressure homogenizer for homogenization treatment under the conditions of 40MPa and 25 ℃; sterilizing the homogenized juice at 600MPa and 20 deg.C by ultra-high pressure technique; filling the sterilized fruit juice in a filling machine, and sealing with a sealing machine; and (4) spraying, cooling and cooling the filled and sealed fruit juice to obtain a finished fruit juice product.
Example 5
Selecting fresh fructus Mali Pumilae and fructus crataegi refrigerated at 4 deg.C, cleaning, removing core, and cutting into pieces; soaking the fragments in 500mg/L ascorbic acid for color protection; weighing 960g of apple fragments and 40g of hawthorn fragments, placing into a low-speed pressure filtration type juicer for juicing, sieving the obtained juice with a 80-mesh sieve, and adding 5% of crude extract of sea buckthorn and 0.1% of anti-browning agent; then sending the mixture into a high-pressure homogenizer for homogenization treatment under the conditions of 40MPa and 25 ℃; pasteurizing the homogenized juice at 72 deg.C for 15 min; filling the sterilized fruit juice in a filling machine, and sealing with a sealing machine; and (4) spraying, cooling and cooling the filled and sealed fruit juice to obtain a finished fruit juice product.
Example 6
The quality characteristics of the juices obtained in examples 1, 2, 3, 4 and 5 were measured and compared to see: compared with the fruit juice obtained by the traditional pasteurization, the fruit juice obtained by the ultrahigh pressure sterilization has better and finer taste, better and brighter color and luster, and no cooking flavor; the NFC apple juice added with the hawthorn and the sea buckthorn not only well keeps the original nutrition and flavor of the apple juice, but also increases the abundant nutrition and pleasant flavor of the hawthorn and the sea buckthorn, and compared with the apple juice without natural ingredients, the NFC apple juice has slower browning and better improves the phenomena of precipitation and layering. Some of the effects can be seen in fig. 1.
To better verify the effectiveness of the newly added anti-browning agent, the following measurements were made for examples 1, 2, 3, 4, 5, and with a blank control (except that the following step of soaking the crumb in 500mg/L ascorbic acid for color protection, compared to example 1) being set up:
the determination method refers to the following steps: selection and application of passion fruit juice browning inhibitor [ J ] Chinese food additive, 2011,06(6):190-,
measurement method reference two: study on the anti-browning effect of Lijiang snow peach through Dongming, Tiansu plum, He Lian Jun.1-MCP and membrane bag treatment [ J ] modern food technology, 2013(08):1796-1799,
TABLE 1 measurement results
Figure BDA0003012812780000061
Figure BDA0003012812780000071
As shown in table 1, the anti-browning agent was additionally added in example 4 compared to the blank control group, and the overall anti-browning effect was further enhanced, specifically, polyphenol oxidase activity was closely related to the browning phenomenon of plant tissues, and the specific activity of polyphenol oxidase of examples 1 to 5 was increased at 15d, but decreased at 25d, wherein the corresponding enzyme activity was most decreased in example 4.
Meanwhile, the components of the anti-browning agent are further proved to play a synergistic effect, and through tests, when only D-tryptophan, chitosan or mannitol is adopted as the anti-browning agent, the specific activity of polyphenol oxidase at 25D is only reduced by about 25%.
While the invention has been described in further detail in connection with specific embodiments thereof, it will be understood that the invention is not limited thereto, and that various other modifications and substitutions may be made by those skilled in the art without departing from the spirit of the invention, which should be considered to be within the scope of the invention as defined by the appended claims.

Claims (4)

1. A preparation method of natural anti-browning NFC apple juice comprises the following steps: (1) pretreatment: preparing and treating seabuckthorn, apples and hawthorns to obtain crude seabuckthorn extract, massive apples and massive hawthorns; (2) preparing juice: preparing the blocky apples and the blocky hawthorns pretreated in the step (1) for juicing, and filtering the squeezed turbid juice to obtain clear juice for later use; adding the seabuckthorn crude extract pretreated in the step (1) and the anti-browning agent into the filtered clear juice to obtain fruit juice; (3) and (3) sterilizing and filling the fruit juice: preparing the fruit juice prepared in the step (2), sending the fruit juice into a high-pressure homogenizer for homogenization treatment, then carrying out ultra-high pressure technical sterilization, then sending the fruit juice into an aseptic filling machine for filling, sealing the opening of the aseptic filling machine by using a packaging machine, and finally carrying out spray cooling to obtain a finished product;
the processing mode of the sea buckthorn in the step (1) is as follows: preparing raw seabuckthorn pulp; taking out the raw juice of seabuckthorn, and adopting flowing water to thaw; filtering the unfrozen raw seabuckthorn pulp by using a 80-mesh screen; centrifuging the filtered seabuckthorn raw pulp; removing impurities of the lower layer precipitate and the upper layer aggregate from the centrifuged raw seabuckthorn pulp, and taking clear liquid of the middle layer; filtering the clear liquid in the middle layer by using a 120-mesh screen to obtain a seabuckthorn crude extract;
the apple and hawthorn processing mode in the step (1) is as follows: preparing apples and hawthorns; cleaning apple and hawthorn, removing core of the apple and the hawthorn, and cutting into blocks; soaking the cut apples and the hawthorn into ascorbic acid solution for color protection;
the anti-browning agent in the step (2) comprises D-tryptophan, chitosan and mannitol, wherein the mass ratio of the D-tryptophan to the chitosan to the mannitol is 2: 17: 10, wherein the addition mass of the anti-browning agent is 0.1 percent of the mass of the massive apples; in the step (2), the adding mass of the blocky hawthorns is 4% of the mass of the blocky apples; mixing and juicing the blocky apples and the blocky hawthorns in the step (2), sieving the squeezed juice with a 80-mesh sieve, and then placing the sieved juice into a container for later use;
the mixing and juicing equipment is a low-speed squeezing type normal juice machine; the adding mass of the seabuckthorn crude extract is 5% of the mass of the filtered clear liquid;
the pressure of the homogenization treatment in the step (3) is 40MPa, and the temperature is set to be 25 ℃;
the sterilization pressure of the ultra-high pressure technique sterilization in the step (3) is 600MPa, the sterilization time is 3min, and the sterilization temperature is below 25 ℃.
2. The method for preparing natural anti-browning NFC apple juice according to claim 1, characterized in that: the storage condition of the raw juice of seabuckthorn is-20 ℃; the centrifugal force of the centrifugal treatment of the raw seabuckthorn pulp is 4800g, and the centrifugal treatment time of the raw seabuckthorn pulp is 15 min.
3. The method for preparing natural anti-browning NFC apple juice according to claim 1, characterized in that: the storage conditions of the apples and the hawthorns are-3-4 ℃; the mass concentration of the ascorbic acid solution for color protection is 500 mg/L.
4. The method for preparing natural anti-browning NFC apple juice according to claim 1, characterized in that: the pressure boosting process of the ultra-high pressure technology sterilization comprises two steps: the first step is to rapidly increase the pressure to 150 MPa; the second step is to increase the pressure to 600MPa at a constant speed within 4 min.
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