CN113068744A - Modified milk powder with antioxidant and anti-aging effects - Google Patents
Modified milk powder with antioxidant and anti-aging effects Download PDFInfo
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- CN113068744A CN113068744A CN202110468535.1A CN202110468535A CN113068744A CN 113068744 A CN113068744 A CN 113068744A CN 202110468535 A CN202110468535 A CN 202110468535A CN 113068744 A CN113068744 A CN 113068744A
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- China
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- solution
- mixed solution
- stirring
- dichloroethane
- milk powder
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- 239000000843 powder Substances 0.000 title claims abstract description 60
- 230000003078 antioxidant effect Effects 0.000 title claims abstract description 42
- 239000003963 antioxidant agent Substances 0.000 title claims abstract description 37
- 235000013336 milk Nutrition 0.000 title claims abstract description 36
- 239000008267 milk Substances 0.000 title claims abstract description 36
- 210000004080 milk Anatomy 0.000 title claims abstract description 36
- 230000003712 anti-aging effect Effects 0.000 title claims abstract description 10
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 claims abstract description 48
- 239000011259 mixed solution Substances 0.000 claims abstract description 38
- 238000003756 stirring Methods 0.000 claims abstract description 38
- 239000012752 auxiliary agent Substances 0.000 claims abstract description 20
- 239000002994 raw material Substances 0.000 claims abstract description 20
- 239000002262 Schiff base Substances 0.000 claims abstract description 13
- 108010046377 Whey Proteins Proteins 0.000 claims abstract description 12
- 102000007544 Whey Proteins Human genes 0.000 claims abstract description 12
- 235000015097 nutrients Nutrition 0.000 claims abstract description 10
- 150000001875 compounds Chemical class 0.000 claims abstract description 8
- 239000012043 crude product Substances 0.000 claims abstract description 8
- 229920001542 oligosaccharide Polymers 0.000 claims abstract description 8
- 150000002482 oligosaccharides Chemical class 0.000 claims abstract description 8
- 150000004753 Schiff bases Chemical class 0.000 claims abstract description 7
- 229920002385 Sodium hyaluronate Polymers 0.000 claims abstract description 7
- DZGCGKFAPXFTNM-UHFFFAOYSA-N ethanol;hydron;chloride Chemical compound Cl.CCO DZGCGKFAPXFTNM-UHFFFAOYSA-N 0.000 claims abstract description 7
- 229940010747 sodium hyaluronate Drugs 0.000 claims abstract description 7
- YWIVKILSMZOHHF-QJZPQSOGSA-N sodium;(2s,3s,4s,5r,6r)-6-[(2s,3r,4r,5s,6r)-3-acetamido-2-[(2s,3s,4r,5r,6r)-6-[(2r,3r,4r,5s,6r)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2- Chemical compound [Na+].CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 YWIVKILSMZOHHF-QJZPQSOGSA-N 0.000 claims abstract description 7
- 239000005862 Whey Substances 0.000 claims abstract description 6
- -1 chitosan oligosaccharide Schiff base Chemical class 0.000 claims abstract description 6
- 229910052500 inorganic mineral Inorganic materials 0.000 claims abstract description 6
- 239000011707 mineral Substances 0.000 claims abstract description 6
- 150000003839 salts Chemical class 0.000 claims abstract description 6
- 235000020183 skimmed milk Nutrition 0.000 claims abstract description 6
- 235000021119 whey protein Nutrition 0.000 claims abstract description 6
- 235000009467 Carica papaya Nutrition 0.000 claims abstract description 5
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 5
- 239000000243 solution Substances 0.000 claims description 89
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 72
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 51
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 36
- LQNUZADURLCDLV-UHFFFAOYSA-N nitrobenzene Chemical compound [O-][N+](=O)C1=CC=CC=C1 LQNUZADURLCDLV-UHFFFAOYSA-N 0.000 claims description 36
- 238000006243 chemical reaction Methods 0.000 claims description 32
- 235000006708 antioxidants Nutrition 0.000 claims description 31
- HUMNYLRZRPPJDN-UHFFFAOYSA-N benzaldehyde Chemical compound O=CC1=CC=CC=C1 HUMNYLRZRPPJDN-UHFFFAOYSA-N 0.000 claims description 30
- SCYULBFZEHDVBN-UHFFFAOYSA-N 1,1-Dichloroethane Chemical compound CC(Cl)Cl SCYULBFZEHDVBN-UHFFFAOYSA-N 0.000 claims description 28
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 21
- 239000000203 mixture Substances 0.000 claims description 18
- 238000002156 mixing Methods 0.000 claims description 17
- QNGNSVIICDLXHT-UHFFFAOYSA-N para-ethylbenzaldehyde Natural products CCC1=CC=C(C=O)C=C1 QNGNSVIICDLXHT-UHFFFAOYSA-N 0.000 claims description 15
- 238000005406 washing Methods 0.000 claims description 15
- 239000007787 solid Substances 0.000 claims description 14
- ULQQGOGMQRGFFR-UHFFFAOYSA-N 2-chlorobenzenecarboperoxoic acid Chemical compound OOC(=O)C1=CC=CC=C1Cl ULQQGOGMQRGFFR-UHFFFAOYSA-N 0.000 claims description 12
- PCYGLFXKCBFGPC-UHFFFAOYSA-N 4-(hydroxymethyl)benzene-1,2-diol Chemical compound OCC1=CC=C(O)C(O)=C1 PCYGLFXKCBFGPC-UHFFFAOYSA-N 0.000 claims description 12
- 102000008186 Collagen Human genes 0.000 claims description 12
- 108010035532 Collagen Proteins 0.000 claims description 12
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 12
- VSCWAEJMTAWNJL-UHFFFAOYSA-K aluminium trichloride Chemical compound Cl[Al](Cl)Cl VSCWAEJMTAWNJL-UHFFFAOYSA-K 0.000 claims description 12
- RQFQJYYMBWVMQG-IXDPLRRUSA-N chitotriose Chemical compound O[C@@H]1[C@@H](N)[C@H](O)O[C@H](CO)[C@H]1O[C@H]1[C@H](N)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)N)[C@@H](CO)O1 RQFQJYYMBWVMQG-IXDPLRRUSA-N 0.000 claims description 12
- 229920001436 collagen Polymers 0.000 claims description 12
- FYSNRJHAOHDILO-UHFFFAOYSA-N thionyl chloride Chemical compound ClS(Cl)=O FYSNRJHAOHDILO-UHFFFAOYSA-N 0.000 claims description 12
- 230000003647 oxidation Effects 0.000 claims description 11
- 238000007254 oxidation reaction Methods 0.000 claims description 11
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 10
- 230000032683 aging Effects 0.000 claims description 10
- 230000000694 effects Effects 0.000 claims description 10
- 238000001914 filtration Methods 0.000 claims description 9
- 238000004806 packaging method and process Methods 0.000 claims description 7
- WSLDOOZREJYCGB-UHFFFAOYSA-N 1,2-Dichloroethane Chemical compound ClCCCl WSLDOOZREJYCGB-UHFFFAOYSA-N 0.000 claims description 6
- AVFZOVWCLRSYKC-UHFFFAOYSA-N 1-methylpyrrolidine Chemical compound CN1CCCC1 AVFZOVWCLRSYKC-UHFFFAOYSA-N 0.000 claims description 6
- AHVYPIQETPWLSZ-UHFFFAOYSA-N N-methyl-pyrrolidine Natural products CN1CC=CC1 AHVYPIQETPWLSZ-UHFFFAOYSA-N 0.000 claims description 6
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 6
- WETWJCDKMRHUPV-UHFFFAOYSA-N acetyl chloride Chemical compound CC(Cl)=O WETWJCDKMRHUPV-UHFFFAOYSA-N 0.000 claims description 6
- 239000012346 acetyl chloride Substances 0.000 claims description 6
- 239000005457 ice water Substances 0.000 claims description 6
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 6
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims description 5
- 240000008042 Zea mays Species 0.000 claims description 5
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 5
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 5
- 235000005822 corn Nutrition 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 5
- 235000021255 galacto-oligosaccharides Nutrition 0.000 claims description 5
- 150000003271 galactooligosaccharides Chemical class 0.000 claims description 5
- 239000008101 lactose Substances 0.000 claims description 5
- 235000010755 mineral Nutrition 0.000 claims description 5
- 239000006188 syrup Substances 0.000 claims description 5
- 235000020357 syrup Nutrition 0.000 claims description 5
- 235000008939 whole milk Nutrition 0.000 claims description 5
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 claims description 4
- 244000189799 Asimina triloba Species 0.000 claims description 4
- 235000006264 Asimina triloba Nutrition 0.000 claims description 4
- 244000181025 Rosa gallica Species 0.000 claims description 4
- 235000000533 Rosa gallica Nutrition 0.000 claims description 4
- 238000010438 heat treatment Methods 0.000 claims description 3
- 238000010992 reflux Methods 0.000 claims description 3
- 229910000029 sodium carbonate Inorganic materials 0.000 claims description 3
- DKKCQDROTDCQOR-UHFFFAOYSA-L Ferrous lactate Chemical compound [Fe+2].CC(O)C([O-])=O.CC(O)C([O-])=O DKKCQDROTDCQOR-UHFFFAOYSA-L 0.000 claims description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 2
- QYSXJUFSXHHAJI-XFEUOLMDSA-N Vitamin D3 Natural products C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C/C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-XFEUOLMDSA-N 0.000 claims description 2
- 229930003427 Vitamin E Natural products 0.000 claims description 2
- BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 claims description 2
- 239000004225 ferrous lactate Substances 0.000 claims description 2
- 235000013925 ferrous lactate Nutrition 0.000 claims description 2
- 229940037907 ferrous lactate Drugs 0.000 claims description 2
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 claims description 2
- 239000008103 glucose Substances 0.000 claims description 2
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 claims description 2
- ZUFQODAHGAHPFQ-UHFFFAOYSA-N pyridoxine hydrochloride Chemical compound Cl.CC1=NC=C(CO)C(CO)=C1O ZUFQODAHGAHPFQ-UHFFFAOYSA-N 0.000 claims description 2
- 229960004172 pyridoxine hydrochloride Drugs 0.000 claims description 2
- 235000019171 pyridoxine hydrochloride Nutrition 0.000 claims description 2
- 239000011764 pyridoxine hydrochloride Substances 0.000 claims description 2
- 229960000342 retinol acetate Drugs 0.000 claims description 2
- QGNJRVVDBSJHIZ-QHLGVNSISA-N retinyl acetate Chemical compound CC(=O)OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C QGNJRVVDBSJHIZ-QHLGVNSISA-N 0.000 claims description 2
- 235000019173 retinyl acetate Nutrition 0.000 claims description 2
- 239000011770 retinyl acetate Substances 0.000 claims description 2
- 239000011781 sodium selenite Substances 0.000 claims description 2
- 229960001471 sodium selenite Drugs 0.000 claims description 2
- 235000015921 sodium selenite Nutrition 0.000 claims description 2
- PPASLZSBLFJQEF-RXSVEWSESA-M sodium-L-ascorbate Chemical compound [Na+].OC[C@H](O)[C@H]1OC(=O)C(O)=C1[O-] PPASLZSBLFJQEF-RXSVEWSESA-M 0.000 claims description 2
- 235000019187 sodium-L-ascorbate Nutrition 0.000 claims description 2
- 239000011755 sodium-L-ascorbate Substances 0.000 claims description 2
- QYSXJUFSXHHAJI-YRZJJWOYSA-N vitamin D3 Chemical compound C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C\C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-YRZJJWOYSA-N 0.000 claims description 2
- 235000005282 vitamin D3 Nutrition 0.000 claims description 2
- 239000011647 vitamin D3 Substances 0.000 claims description 2
- 235000019165 vitamin E Nutrition 0.000 claims description 2
- 229940046009 vitamin E Drugs 0.000 claims description 2
- 239000011709 vitamin E Substances 0.000 claims description 2
- 229940021056 vitamin d3 Drugs 0.000 claims description 2
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 2
- 229960001763 zinc sulfate Drugs 0.000 claims description 2
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 2
- 239000002671 adjuvant Substances 0.000 claims 1
- 230000002421 anti-septic effect Effects 0.000 abstract description 4
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical group OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 abstract description 3
- 244000000231 Sesamum indicum Species 0.000 abstract description 3
- 235000003434 Sesamum indicum Nutrition 0.000 abstract description 3
- 231100000956 nontoxicity Toxicity 0.000 abstract description 3
- 239000003242 anti bacterial agent Substances 0.000 abstract description 2
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 2
- 240000006432 Carica papaya Species 0.000 abstract 1
- 241000220317 Rosa Species 0.000 abstract 1
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 18
- 238000000034 method Methods 0.000 description 14
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 11
- 229920002674 hyaluronan Polymers 0.000 description 11
- 229960003160 hyaluronic acid Drugs 0.000 description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 11
- 230000008569 process Effects 0.000 description 10
- 239000000706 filtrate Substances 0.000 description 8
- 230000007935 neutral effect Effects 0.000 description 8
- 210000003491 skin Anatomy 0.000 description 7
- 239000008367 deionised water Substances 0.000 description 6
- 229910021641 deionized water Inorganic materials 0.000 description 6
- 239000002244 precipitate Substances 0.000 description 6
- 238000002390 rotary evaporation Methods 0.000 description 6
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 4
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 4
- 238000000944 Soxhlet extraction Methods 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 239000012074 organic phase Substances 0.000 description 4
- 230000000750 progressive effect Effects 0.000 description 4
- 238000007873 sieving Methods 0.000 description 4
- 239000002904 solvent Substances 0.000 description 4
- 238000005303 weighing Methods 0.000 description 4
- 235000019441 ethanol Nutrition 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 238000001291 vacuum drying Methods 0.000 description 3
- 241000237519 Bivalvia Species 0.000 description 2
- 238000010934 O-alkylation reaction Methods 0.000 description 2
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 2
- 125000003277 amino group Chemical group 0.000 description 2
- 230000002155 anti-virotic effect Effects 0.000 description 2
- 150000005528 benzodioxoles Chemical class 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
- 239000012295 chemical reaction liquid Substances 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 230000006866 deterioration Effects 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 239000012065 filter cake Substances 0.000 description 2
- 238000009920 food preservation Methods 0.000 description 2
- 238000000227 grinding Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 150000004968 peroxymonosulfuric acids Chemical class 0.000 description 2
- 239000003208 petroleum Substances 0.000 description 2
- 230000001766 physiological effect Effects 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 230000000644 propagated effect Effects 0.000 description 2
- 235000017557 sodium bicarbonate Nutrition 0.000 description 2
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 2
- GEHJYWRUCIMESM-UHFFFAOYSA-L sodium sulfite Chemical class [Na+].[Na+].[O-]S([O-])=O GEHJYWRUCIMESM-UHFFFAOYSA-L 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000000967 suction filtration Methods 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 238000003828 vacuum filtration Methods 0.000 description 2
- CHHHXKFHOYLYRE-UHFFFAOYSA-M 2,4-Hexadienoic acid, potassium salt (1:1), (2E,4E)- Chemical group [K+].CC=CC=CC([O-])=O CHHHXKFHOYLYRE-UHFFFAOYSA-M 0.000 description 1
- 102000006587 Glutathione peroxidase Human genes 0.000 description 1
- 108700016172 Glutathione peroxidases Proteins 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 102000019197 Superoxide Dismutase Human genes 0.000 description 1
- 108010012715 Superoxide dismutase Proteins 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000007792 addition Methods 0.000 description 1
- 206010003246 arthritis Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000000845 cartilage Anatomy 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 238000010511 deprotection reaction Methods 0.000 description 1
- 210000004207 dermis Anatomy 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 210000001508 eye Anatomy 0.000 description 1
- 230000001815 facial effect Effects 0.000 description 1
- 235000013373 food additive Nutrition 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000004302 potassium sorbate Substances 0.000 description 1
- 235000010241 potassium sorbate Nutrition 0.000 description 1
- 229940069338 potassium sorbate Drugs 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000007665 sagging Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 230000009759 skin aging Effects 0.000 description 1
- 230000037394 skin elasticity Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 210000003954 umbilical cord Anatomy 0.000 description 1
- 230000037303 wrinkles Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/152—Milk preparations; Milk powder or milk powder preparations containing additives
- A23C9/1526—Amino acids; Peptides; Protein hydrolysates; Nucleic acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C3/00—Preservation of milk or milk preparations
- A23C3/08—Preservation of milk or milk preparations by addition of preservatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/152—Milk preparations; Milk powder or milk powder preparations containing additives
- A23C9/156—Flavoured milk preparations ; Addition of fruits, vegetables, sugars, sugar alcohols or sweeteners
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses a modified milk powder with antioxidant and anti-aging effects, which comprises the following raw materials: desalted whey powder, concentrated whey protein powder, sodium hyaluronate, papaya fruit powder, rose powder with double red petals, milk mineral salt, compound nutrient package, antioxidant component, skimmed milk powder and the like; the antioxidant component is prepared by the following steps: step one, preparing chitosan oligosaccharide Schiff base; secondly, dissolving the auxiliary agent in dimethylformamide to obtain a mixed solution A, dissolving the Schiff base of the oligosaccharides in dimethylformamide to obtain a mixed solution B, then dropwise adding the mixed solution B into the mixed solution A, and reacting to obtain a crude product; and thirdly, adding a hydrochloric acid ethanol mixed solution into the crude product obtained in the second step, stirring at room temperature, and treating to obtain the antioxidant component. The auxiliary agent contains a sesame phenol analog structure, the oligosaccharide is used as a natural antiseptic antibacterial agent, and besides biodegradability, biocompatibility and biological non-toxicity, the auxiliary agent with antibacterial activity is introduced to the chain, so that a high-efficiency antioxidant component is obtained.
Description
Technical Field
The invention belongs to the technical field of food, and particularly relates to modified milk powder with antioxidant and anti-aging effects.
Background
The modified milk powder is a powdery product prepared by processing raw cow (sheep) milk or a processed product thereof serving as a main raw material, adding other raw materials, and optionally adding a food additive and a nutrition enhancer, and the conventional modified milk powder still needs to be improved. In addition, hyaluronic acid is also commonly known as Hyaluronic Acid (HA), and is widely present in tissues such as joint cavities, skin, vitreous eye, cartilage, umbilical cord, etc. of the human body. The content of hyaluronic acid in human body gradually decreases with age, and when the age is 30, 50 and 60 years old, the content of hyaluronic acid in human body decreases to 65%, 45% and 25% of 20 years old respectively. The reduction of hyaluronic acid can lead to arthritis, skin aging, increased wrinkles, and eye puffiness.
The problems of preservation and oxidation resistance of food are key to food preservation. Because the prepared milk powder contains a large amount of nutrient substances, the prepared milk powder is very suitable for the growth of microorganisms. If the modified milk powder is not preserved properly, a large number of microorganisms will be propagated, resulting in putrefaction and deterioration.
Disclosure of Invention
The invention provides modified milk powder with antioxidant and anti-aging effects.
The technical problems to be solved by the invention are as follows:
the problems of preservation and oxidation resistance of food are key to food preservation. Because the prepared milk powder contains a large amount of nutrient substances, the prepared milk powder is very suitable for the growth of microorganisms. If the modified milk powder is not preserved properly, a large number of microorganisms will be propagated, resulting in putrefaction and deterioration.
The purpose of the invention can be realized by the following technical scheme:
the modified milk powder with the effects of resisting oxidation and ageing comprises the following raw materials in percentage by weight:
20-26% of desalted whey powder, 18-20% of whole milk powder, 10-16% of solid corn syrup, 3-7% of lactose, 3-6% of collagen peptide, 3-6% of galacto-oligosaccharide, 3-5% of concentrated whey protein powder, 0.1-0.4% of sodium hyaluronate, 0-0.3% of pawpaw fruit powder, 0-0.3% of double red rose pollen, 0.3-0.6% of milk mineral salt, 0.5% of compound nutrient package, 0.2-0.6% of antioxidant component and the balance of skimmed milk powder to be 100%;
accurately weighing the raw materials according to the formula, respectively sieving the raw materials with a 60-80 mesh sieve, adding the raw materials into a mixer according to an equivalent progressive method, uniformly mixing, and directly packaging to obtain the modified milk powder with the effects of resisting oxidation and ageing.
The antioxidant component is prepared by the following steps:
dissolving chitosan oligosaccharide in an acetic acid solution, adding methanol, uniformly stirring, dropwise adding a methanol solution of benzaldehyde at the temperature of 60 ℃, stirring and reacting at the constant temperature of 60 ℃, and performing aftertreatment after the reaction is finished, wherein the aftertreatment process comprises the following steps: cooling the reaction liquid to room temperature, volatilizing the organic solvent, adjusting the pH value to 7 by using a sodium hydroxide solution with the mass fraction of 5%, continuously stirring until no flocculent precipitate is generated, performing suction filtration, collecting the precipitate, performing Soxhlet extraction on the precipitate by using absolute ethyl alcohol for 12h, washing to be neutral, performing vacuum drying, and grinding to obtain powder, thereby obtaining the chitosan oligosaccharide Schiff base;
the reaction process is as follows:
and secondly, dissolving an auxiliary agent in dimethylformamide to obtain a mixed solution A, dissolving the Schiff base of the oligosaccharides in dimethylformamide to obtain a mixed solution B, then dropwise adding the mixed solution B into the mixed solution A, stirring and reacting for 6 hours at the temperature of 25 ℃, and performing post-treatment after the reaction is finished, wherein the post-treatment process comprises the following steps: adding excessive acetone to terminate the reaction, generating solid, performing vacuum filtration, performing Soxhlet extraction on the obtained solid with acetone, removing dimethylformamide, and finally performing vacuum drying to obtain a crude product;
the reaction process is as follows:
and thirdly, adding a hydrochloric acid ethanol mixed solution into the crude product obtained in the second step, stirring at room temperature for 12 hours, adjusting the pH value to 7 by using sodium carbonate, washing by using acetone, filtering, and drying in vacuum to obtain the antioxidant component.
The reaction process is as follows:
further, the mass fraction of the acetic acid solution in the first step is 1%, and the methanol solution of benzaldehyde is prepared by mixing benzaldehyde and methanol according to a volume ratio of 1: 10, mixing the chitosan oligosaccharide, the acetic acid solution and the methanol solution of methanol and benzaldehyde, wherein the dosage ratio of the chitosan oligosaccharide, the acetic acid solution and the methanol solution of methanol to benzaldehyde is 15 g: 70mL of: 120mL of: 120 mL; in the second step, the mixed solution A is taken as an auxiliary agent and dimethylformamide according to the weight ratio of 1 g: 10mL, wherein the mixed solution B is formed by mixing the schiff base of the sub-packaging oligosaccharide and dimethylformamide according to the weight ratio of 1 g: 10mL of the mixture is mixed, and the volume ratio of the mixed solution A to the mixed solution B is 1: 3; in the third step, the hydrochloric acid-ethanol mixed solution is 0.25mol/L hydrochloric acid solution and absolute ethanol according to the volume ratio of 1: 4, and mixing.
Further, the auxiliary agent is prepared by the following steps:
step S11, adding 1, 2-dichloroethane and N-methylpyrrolidine into a three-neck flask, heating to reflux temperature, dropwise adding a mixed solution of 3, 4-dihydroxybenzyl alcohol and sodium hydroxide solution while stirring, stirring and reacting for 10 hours at the temperature of 91 ℃ after dropwise adding, and performing post-treatment after the reaction is finished, wherein the post-treatment process comprises the following steps: firstly, decompressing and filtering, decompressing and concentrating the obtained filtrate to recover unreacted dichloroethane solution, and then drying the dichloroethane solution by using anhydrous sodium sulfate to obtain an intermediate 1;
the reaction process is as follows:
step S12, adding the intermediate 1 and thionyl chloride into a flask, stirring for reaction, stirring at room temperature until solid is precipitated, and performing post-treatment, wherein the post-treatment process comprises the following steps: adding petroleum ether, washing, filtering, discarding filtrate until the washing liquid is colorless, and recrystallizing the obtained filter cake in boiling water to obtain an intermediate 2;
the reaction process is as follows:
step S13, adding anhydrous aluminum chloride and nitrobenzene into a three-neck flask, adding acetyl chloride under the condition of ice-water bath, stirring and reacting for 30min, then adding a nitrobenzene solution of the intermediate 2, removing the ice-water bath after dropwise adding, stirring and reacting for 16h under the condition of room temperature, and performing post-treatment after the reaction is finished, wherein the post-treatment specific steps are as follows: mixing the obtained reaction solution with a hydrochloric acid solution with the mass fraction of 10%, extracting with dichloromethane, washing the obtained organic phase with deionized water, a sodium hydroxide solution with the mass fraction of 5% and deionized water to be neutral, performing rotary evaporation to remove dichloromethane to obtain a solid, and recrystallizing with an ethanol solution with the volume fraction of 40% to obtain an intermediate 3;
the reaction process is as follows:
step S14, adding the dichloroethane solution of the intermediate 3 into a three-neck flask, dropwise adding the dichloroethane solution of chloroperoxybenzoic acid at 0 ℃, stirring and reacting for 10 hours at room temperature, and carrying out post-treatment after the reaction is finished, wherein the post-treatment process comprises the following steps: filtering, washing the obtained filtrate with saturated sodium sulfite, a sodium hydroxide solution with the mass fraction of 10% and distilled water respectively to be neutral, then carrying out rotary evaporation to remove the solvent, then adding the filtrate into a sodium hydroxide solution with the mass fraction of 15%, stirring the mixture at room temperature until the mixture is dissolved and saponified, adjusting the pH value to 3 with persulfuric acid, extracting the mixture with dichloromethane, washing an organic phase with a sodium bicarbonate solution with the mass fraction of 15% and deionized water to be neutral, and carrying out rotary evaporation to remove the solvent to obtain the auxiliary agent.
The reaction process is as follows:
further, the mass fraction of the sodium hydroxide solution in step S11 is 50%; the dosage ratio of the 1, 2-dichloroethane, the N-methylpyrrolidine, the 3, 4-dihydroxybenzyl alcohol and the sodium hydroxide solution is 80 mL: 150mL of: 28 g: 30 mL;
in the step S12, the dosage mass ratio of the intermediate 1 to the thionyl chloride is 16: 12;
the dosage ratio of the anhydrous aluminum chloride, nitrobenzene, acetyl chloride and the nitrobenzene solution of the intermediate 2 in the step S13 was 22.2 g: 250mL of: 20mL of: 50 mL; the nitrobenzene solution of intermediate 2 was intermediate 2 and nitrobenzene solution in 19 g: 50mL of the mixture is mixed;
the dichloroethane solution of chloroperoxybenzoic acid in step S14 was prepared from chloroperoxybenzoic acid and dichloroethane in an amount of 2.8 g: 30mL of the mixture, and the dichloroethane solution of the intermediate 3 is the mixture of the intermediate 3 and dichloroethane according to a ratio of 2.2 g: 50mL of the intermediate 3, and the volume ratio of the dichloroethane solution of chloroperoxybenzoic acid to the dichloroethane solution of the intermediate 3 is 3: 5.
further, the compound nutrient package contains 1000g of vitamin A acetate: 0.2-1.13g, vitamin D3: 0.00042-0.00133g, vitamin E: 0.6-1.47g, pyridoxine hydrochloride: 0.6-1.47g, sodium L-ascorbate: 0.6-01.47g, ferrous lactate: 0.8-1.14g, zinc sulfate: 0.8-1.14g and sodium selenite: 0.3-1 g; the balance is glucose to make up to 1000 g.
The invention has the beneficial effects that:
although both collagen and hyaluronic acid are located in the dermis, collagen more like an "elastic mesh" maintains facial elasticity and helps the skin resist sagging and wrinkling. The collagen fiber net formed by the collagen in the skin structure provides a basic structure for the hyaluronic acid, so that the hyaluronic acid has a better water retention effect on the skin and helps the skin to moisturize and moisturize. Meanwhile, the hyaluronic acid also helps the collagen fiber net to be better stretched in water and environment, and the collagen and the hyaluronic acid provide nutrition for the skin.
The prepared milk powder added with the collagen peptide and the sodium hyaluronate can be absorbed by human tissues; increasing skin moisture; the content of superoxide dismutase and glutathione peroxidase in serum is increased, and the antioxidant effect is achieved; increasing skin elasticity and resisting aging.
The auxiliary agent contains a sesame phenol analog structure, has good safety and antioxidant activity, can effectively remove free radicals, has antioxidant activity in a phenolic hydroxyl structure, shows multiple physiological activities of bacteriostasis, antivirus and the like in a benzodioxole derivative, is introduced into an antioxidant component, improves the action of the antioxidant component, has an O-alkylation reaction with chitosan oligosaccharide Schiff base, is introduced onto a chitosan oligosaccharide molecular chain, is deprotected by using an acid-alcohol solution, releases an active amino group, obtains the antioxidant component, is used as a natural antiseptic antibacterial agent, has biodegradability, biocompatibility and biological non-toxicity, and is introduced onto the chain to obtain a high-efficiency antioxidant component.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
The auxiliary agent is prepared by the following steps:
step S11, adding 1, 2-dichloroethane and N-methylpyrrolidine into a three-neck flask, heating to reflux temperature, dropwise adding a mixed solution of 3, 4-dihydroxybenzyl alcohol and sodium hydroxide solution while stirring, stirring and reacting for 10 hours at the temperature of 91 ℃ after dropwise adding, and performing post-treatment after the reaction is finished, wherein the post-treatment process comprises the following steps: firstly, decompressing and filtering, decompressing and concentrating the obtained filtrate to recover unreacted dichloroethane solution, and then drying the dichloroethane solution by using anhydrous sodium sulfate to obtain an intermediate 1;
step S12, adding the intermediate 1 and thionyl chloride into a flask, stirring for reaction, stirring at room temperature until solid is precipitated, and performing post-treatment, wherein the post-treatment process comprises the following steps: adding petroleum ether, washing, filtering, discarding filtrate until the washing liquid is colorless, and recrystallizing the obtained filter cake in boiling water to obtain an intermediate 2;
step S13, adding anhydrous aluminum chloride and nitrobenzene into a three-neck flask, adding acetyl chloride under the condition of ice-water bath, stirring for reaction for 30min, then adding a nitrobenzene solution of the intermediate 2, removing the ice-water bath after dropwise adding, stirring for reaction for 16h under the condition of room temperature, and performing post-treatment after the reaction is finished, wherein the post-treatment specific steps are as follows: mixing the obtained reaction solution with a hydrochloric acid solution with the mass fraction of 10%, extracting with dichloromethane, washing the obtained organic phase with deionized water, a sodium hydroxide solution with the mass fraction of 5% and deionized water to be neutral, performing rotary evaporation to remove dichloromethane to obtain a solid, and recrystallizing with an ethanol solution with the volume fraction of 40% to obtain an intermediate 3;
step S14, adding the dichloroethane solution of the intermediate 3 into a three-neck flask, dropwise adding the dichloroethane solution of chloroperoxybenzoic acid at 0 ℃, stirring and reacting for 10 hours at room temperature, and carrying out post-treatment after the reaction is finished, wherein the post-treatment process comprises the following steps: filtering, washing the obtained filtrate with saturated sodium sulfite, a sodium hydroxide solution with the mass fraction of 10% and distilled water respectively to be neutral, then carrying out rotary evaporation to remove the solvent, then adding the filtrate into a sodium hydroxide solution with the mass fraction of 15%, stirring the mixture at room temperature until the mixture is dissolved and saponified, adjusting the pH value to 3 with persulfuric acid, extracting the mixture with dichloromethane, washing an organic phase with a sodium bicarbonate solution with the mass fraction of 15% and deionized water to be neutral, and carrying out rotary evaporation to remove the solvent to obtain the auxiliary agent.
Wherein the mass fraction of the sodium hydroxide solution in the step S11 is 50%; the dosage ratio of the 1, 2-dichloroethane, the N-methylpyrrolidine, the 3, 4-dihydroxybenzyl alcohol and the sodium hydroxide solution is 80 mL: 150mL of: 28 g: 30 mL;
in the step S12, the dosage mass ratio of the intermediate 1 to the thionyl chloride is 16: 12;
the dosage ratio of the anhydrous aluminum chloride, nitrobenzene, acetyl chloride and the nitrobenzene solution of the intermediate 2 in the step S13 was 22.2 g: 250mL of: 20mL of: 50 mL; the nitrobenzene solution of intermediate 2 was intermediate 2 and nitrobenzene solution in 19 g: 50mL of the mixture is mixed;
the dichloroethane solution of chloroperoxybenzoic acid in step S14 was prepared from chloroperoxybenzoic acid and dichloroethane in an amount of 2.8 g: 30mL of the mixture, and the dichloroethane solution of the intermediate 3 is the mixture of the intermediate 3 and dichloroethane according to a ratio of 2.2 g: 50mL of the intermediate 3, and the volume ratio of the dichloroethane solution of chloroperoxybenzoic acid to the dichloroethane solution of the intermediate 3 is 3: 5.
example 2
The antioxidant component is prepared by the following steps:
dissolving chitosan oligosaccharide in an acetic acid solution, adding methanol, uniformly stirring, dropwise adding a methanol solution of benzaldehyde at the temperature of 60 ℃, stirring and reacting at the constant temperature of 60 ℃, and performing aftertreatment after the reaction is finished, wherein the aftertreatment process comprises the following steps: cooling the reaction liquid to room temperature, volatilizing the organic solvent, adjusting the pH value to 7 by using a sodium hydroxide solution with the mass fraction of 5%, continuously stirring until no flocculent precipitate is generated, performing suction filtration, collecting the precipitate, performing Soxhlet extraction on the precipitate by using absolute ethyl alcohol for 12h, washing to be neutral, performing vacuum drying, and grinding to obtain powder, thereby obtaining the chitosan oligosaccharide Schiff base;
and secondly, dissolving an auxiliary agent in dimethylformamide to obtain a mixed solution A, dissolving the Schiff base of the oligosaccharides in dimethylformamide to obtain a mixed solution B, then dropwise adding the mixed solution B into the mixed solution A, stirring and reacting for 6 hours at the temperature of 25 ℃, and performing post-treatment after the reaction is finished, wherein the post-treatment process comprises the following steps: adding excessive acetone to terminate the reaction, generating solid, performing vacuum filtration, performing Soxhlet extraction on the obtained solid with acetone, and removing dimethylformamide to obtain a crude product;
and thirdly, adding a hydrochloric acid ethanol mixed solution into the crude product obtained in the second step, stirring at room temperature for 12 hours, adjusting the pH value to 7 by using sodium carbonate, washing by using acetone, filtering, and drying in vacuum to obtain the antioxidant component.
Wherein, the mass fraction of the acetic acid solution in the first step is 1%, and the methanol solution of benzaldehyde is prepared by mixing benzaldehyde and methanol according to the volume ratio of 1: 10, mixing the chitosan oligosaccharide, the acetic acid solution and the methanol solution of methanol and benzaldehyde, wherein the dosage ratio of the chitosan oligosaccharide, the acetic acid solution and the methanol solution of methanol to benzaldehyde is 15 g: 70mL of: 120mL of: 120 mL; in the second step, the mixed solution A is taken as an auxiliary agent and dimethylformamide according to the weight ratio of 1 g: 10mL, wherein the mixed solution B is formed by mixing the schiff base of the sub-packaging oligosaccharide and dimethylformamide according to the weight ratio of 1 g: 10mL of the mixture is mixed, and the volume ratio of the mixed solution A to the mixed solution B is 1: 3; in the third step, the hydrochloric acid-ethanol mixed solution is 0.25mol/L hydrochloric acid solution and absolute ethanol according to the volume ratio of 1: 4, wherein the auxiliary agent is prepared in the example 1.
Example 3
The modified milk powder with the effects of resisting oxidation and ageing comprises the following raw materials in percentage by weight:
20% of desalted whey powder, 18% of whole milk powder, 10% of solid corn syrup, 3% of lactose, 3% of collagen peptide, 3% of galacto-oligosaccharide, 3% of concentrated whey protein powder, 0.1% of sodium hyaluronate, 0.3% of milk mineral salt, 0.5% of compound nutrient package, 0.2% of antioxidant component and the balance of skimmed milk powder to make up to 100%; wherein the antioxidant component is obtained in example 2.
Accurately weighing the raw materials according to the formula, respectively sieving the raw materials with a 60-mesh sieve, adding the raw materials into a mixer according to an equivalent progressive method, uniformly mixing, and directly packaging to obtain the modified milk powder with the effects of resisting oxidation and resisting aging.
Example 4
The modified milk powder with the effects of resisting oxidation and ageing comprises the following raw materials in percentage by weight:
23% of desalted whey powder, 19% of whole milk powder, 3% of solid corn syrup, 5% of lactose, 4% of collagen peptide, 5% of galacto-oligosaccharide, 4% of concentrated whey protein powder, 0.2% of sodium hyaluronate, 0.2% of pawpaw fruit powder, 0.2% of bivalvia red rose pollen, 0.5% of milk mineral salt, 0.5% of compound nutrient package, 0.5% of antioxidant component and the balance of skimmed milk powder to be 100%; wherein the antioxidant component is obtained in example 2.
Accurately weighing the raw materials according to the formula, respectively sieving the raw materials with a 70-mesh sieve, adding the raw materials into a mixer according to an equivalent progressive method, uniformly mixing, and directly packaging to obtain the modified milk powder with the effects of resisting oxidation and resisting aging.
Example 5
The modified milk powder with the effects of resisting oxidation and ageing comprises the following raw materials in percentage by weight:
26% of desalted whey powder, 20% of whole milk powder, 16% of solid corn syrup, 7% of lactose, 6% of collagen peptide, 6% of galacto-oligosaccharide, 5% of concentrated whey protein powder, 0.4% of sodium hyaluronate, 0.3% of pawpaw fruit powder, 0.3% of bivalvia red rose pollen, 0.6% of milk mineral salt, 0.5% of compound nutrient package, 0.6% of antioxidant component and the balance of skimmed milk powder to be 100%; wherein the antioxidant component is obtained in example 2.
Accurately weighing the raw materials according to the formula, respectively sieving the raw materials with a 80-mesh sieve, adding the raw materials into a mixer according to an equivalent progressive method, uniformly mixing, and directly packaging to obtain the modified milk powder with the effects of resisting oxidation and resisting aging.
Comparative example 1
The antioxidant component of example 3 was replaced with potassium sorbate, the remainder being unchanged.
The milk powder of examples 3-5 and comparative example 1 was subjected to a corrosion resistance test; the results of the microbial detection in the storage experiment are shown in table 1 below:
TABLE 1
From the above table 1, it can be seen that the modified milk powder with antioxidant and anti-aging effects prepared by the invention has long storage time, the auxiliary agent contains a sesame phenol analog structure, has good safety and antioxidant activity, can effectively remove free radicals, the phenolic hydroxyl structure has antioxidant activity, the benzodioxole derivative also shows multiple physiological activities such as bacteriostasis, antivirus and the like, the auxiliary agent is introduced into the antioxidant component to improve the antiseptic effect of the antioxidant component, the auxiliary agent and chitosan oligosaccharide schiff base undergo an O-alkylation reaction, the auxiliary agent is introduced onto the chitosan oligosaccharide molecular chain, then the acid alcohol solution is used for deprotection to release active amino groups to obtain the antioxidant component, the chitosan oligosaccharide is used as a natural antiseptic, besides biodegradability, biocompatibility and biological non-toxicity, the auxiliary agent with antibacterial activity is introduced onto the chain to obtain a high-efficiency antioxidant component, the storage time is improved.
In the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The foregoing is illustrative and explanatory only and is not intended to be exhaustive or to limit the invention to the precise embodiments described, and various modifications, additions, and substitutions may be made by those skilled in the art without departing from the scope of the invention or exceeding the scope of the claims.
Claims (5)
1. The modified milk powder with the effects of resisting oxidation and ageing is characterized by comprising the following raw materials in percentage by weight:
20-26% of desalted whey powder, 18-20% of whole milk powder, 10-16% of solid corn syrup, 3-7% of lactose, 3-6% of collagen peptide, 3-6% of galacto-oligosaccharide, 3-5% of concentrated whey protein powder, 0.1-0.4% of sodium hyaluronate, 0-0.3% of pawpaw fruit powder, 0-0.3% of double red rose pollen, 0.3-0.6% of milk mineral salt, 0.5% of compound nutrient package, 0.2-0.6% of antioxidant component and the balance of skimmed milk powder to be 100%;
the antioxidant component is prepared by the following steps:
dissolving chitosan oligosaccharide in an acetic acid solution, adding methanol, stirring uniformly, dropwise adding a methanol solution of benzaldehyde at the temperature of 60 ℃, stirring at the constant temperature of 60 ℃ for reaction, and performing post-treatment after the reaction is finished to obtain chitosan oligosaccharide Schiff base;
secondly, dissolving an auxiliary agent in dimethylformamide to obtain a mixed solution A, dissolving the Schiff base of the oligosaccharides in dimethylformamide to obtain a mixed solution B, then dropwise adding the mixed solution B into the mixed solution A, stirring and reacting for 6 hours at the temperature of 25 ℃, and performing post-treatment after the reaction is finished to obtain a crude product;
and thirdly, adding a hydrochloric acid ethanol mixed solution into the crude product obtained in the second step, stirring at room temperature for 12 hours, adjusting the pH value to 7 by using sodium carbonate, washing by using acetone, filtering, and drying in vacuum to obtain the antioxidant component.
2. The modified milk powder with antioxidant and anti-aging effects as claimed in claim 1, wherein the mass fraction of the acetic acid solution in the first step is 1%, and the methanol solution of benzaldehyde is prepared by mixing benzaldehyde and methanol according to a volume ratio of 1: 10, mixing the chitosan oligosaccharide, the acetic acid solution and the methanol solution of methanol and benzaldehyde, wherein the dosage ratio of the chitosan oligosaccharide, the acetic acid solution and the methanol solution of methanol to benzaldehyde is 15 g: 70mL of: 120mL of: 120 mL; in the second step, the mixed solution A is taken as an auxiliary agent and dimethylformamide according to the weight ratio of 1 g: 10mL, wherein the mixed solution B is formed by mixing the schiff base of the sub-packaging oligosaccharide and dimethylformamide according to the weight ratio of 1 g: 10mL of the mixture is mixed, and the volume ratio of the mixed solution A to the mixed solution B is 1: 3; in the third step, the hydrochloric acid-ethanol mixed solution is 0.25mol/L hydrochloric acid solution and absolute ethanol according to the volume ratio of 1: 4, and mixing.
3. The modified milk powder with antioxidant and antiaging effects as claimed in claim 2, characterized in that the adjuvant is prepared by the following steps:
step S11, adding 1, 2-dichloroethane and N-methylpyrrolidine into a three-neck flask, heating to a reflux temperature, then dropwise adding a mixed solution of 3, 4-dihydroxybenzyl alcohol and a sodium hydroxide solution while stirring, stirring and reacting for 10 hours at the temperature of 91 ℃ after dropwise adding, and performing post-treatment after the reaction is finished to obtain an intermediate 1;
step S12, adding the intermediate 1 and thionyl chloride into a flask, stirring for reaction, stirring at room temperature until solid is precipitated, and performing aftertreatment to obtain an intermediate 2;
step S13, adding anhydrous aluminum chloride and nitrobenzene into a three-neck flask, adding acetyl chloride under the condition of ice-water bath, stirring and reacting for 30min, then adding a nitrobenzene solution of the intermediate 2, removing the ice-water bath after dropwise adding, stirring and reacting for 16h under the condition of room temperature, and performing post-treatment after the reaction is finished to obtain an intermediate 3;
and step S14, adding the dichloroethane solution of the intermediate 3 into a three-neck flask, dropwise adding the dichloroethane solution of chloroperoxybenzoic acid at 0 ℃, stirring and reacting for 10 hours at room temperature, and performing post-treatment after the reaction is finished to obtain the assistant.
4. The modified milk powder with antioxidant and anti-aging effects as claimed in claim 3, wherein the mass fraction of the sodium hydroxide solution in step S11 is 50%; the dosage ratio of the 1, 2-dichloroethane, the N-methylpyrrolidine, the 3, 4-dihydroxybenzyl alcohol and the sodium hydroxide solution is 80 mL: 150mL of: 28 g: 30 mL; in the step S12, the dosage mass ratio of the intermediate 1 to the thionyl chloride is 16: 12; the dosage ratio of the anhydrous aluminum chloride, nitrobenzene, acetyl chloride and the nitrobenzene solution of the intermediate 2 in the step S13 was 22.2 g: 250mL of: 20mL of: 50 mL; the nitrobenzene solution of intermediate 2 was intermediate 2 and nitrobenzene solution in the following weight ratio of 19 g: 50mL of the mixture is mixed; the dichloroethane solution of chloroperoxybenzoic acid in step S14 was prepared from chloroperoxybenzoic acid and dichloroethane in an amount of 2.8 g: 30mL of the mixture, and the dichloroethane solution of the intermediate 3 is the mixture of the intermediate 3 and dichloroethane according to a ratio of 2.2 g: 50mL of the intermediate 3, and the volume ratio of the dichloroethane solution of chloroperoxybenzoic acid to the dichloroethane solution of the intermediate 3 is 3: 5.
5. the modified milk powder with antioxidant and anti-aging effects as claimed in claim 1, wherein the compound nutrient package contains 1000g of vitamin A acetate: 0.2-1.13g, vitamin D3: 0.00042-0.00133g, vitamin E: 0.6-1.47g, pyridoxine hydrochloride: 0.6-1.47g, sodium L-ascorbate: 0.6-01.47g, ferrous lactate: 0.8-1.14g, zinc sulfate: 0.8-1.14g and sodium selenite: 0.3-1 g; the balance is glucose to make up to 1000 g.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115644258A (en) * | 2021-12-31 | 2023-01-31 | 君乐宝乳业集团有限公司 | Health milk powder for women and its preparation method |
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