CN113049561A - Compressed light confocal detection device and method - Google Patents
Compressed light confocal detection device and method Download PDFInfo
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- CN113049561A CN113049561A CN202110315599.8A CN202110315599A CN113049561A CN 113049561 A CN113049561 A CN 113049561A CN 202110315599 A CN202110315599 A CN 202110315599A CN 113049561 A CN113049561 A CN 113049561A
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Abstract
The invention discloses a compressed light confocal detection device, which comprises: the device comprises a compressed light emission source and a confocal detection module; the compressed light emission source is connected with the confocal detection module and used for generating compressed light and enabling the compressed light to enter the confocal detection module; the confocal detection module is used for imaging a sample by using the compressed light. The invention combines the compressed light and the confocal light, utilizes the compressed light generated by the compressed light emitting source to detect the sample, greatly improves the resolution ratio of the imaging of the sample, and simultaneously adopts the confocal light to effectively reduce the interference of the non-focal plane noise and improve the imaging quality.
Description
Technical Field
The invention relates to the field of microscopic detection, in particular to a compressed light confocal detection device and method.
Background
In the field of microscopic detection, increasing the detection thickness of a sample, improving resolution and imaging quality have been targets pursued by scientists. For the detection of a sample, a receiver is difficult to distinguish photons carrying important information and scattered noise photons, the two photons are identical in momentum space, a confocal microscope forms a point light source by utilizing a scanning light beam through a grating pinhole, the point-by-point scanning is carried out on a focal plane of a fluorescence labeling sample, an optical signal of an acquisition point reaches the receiver through a detection pinhole, an image is formed on a computer monitoring screen after signal processing, light emitted from the focal point of an objective focal plane can be well converged at the pinhole and can be completely received by the detector through the pinhole, light emitted from the upper position and the lower position of the focal plane can generate light spots with large diameters at the pinhole, and compared with the diameter of the pinhole, only a small part of light can be received by the detector through the pinhole. And with the distance from the focal plane of the objective lens being larger, the scattered light generated by the sample at the pinhole is larger, the energy capable of penetrating through the pinhole is smaller (from 10% to 1%, slowly approaching to 0%), the signal generated on the detector is smaller, and the influence is smaller, so that the confocal microscope only images the focal plane of the sample, the interference of stray light such as scattered noise photons and the like can be effectively avoided, and the resolution ratio of the confocal microscope is higher than that of a common microscope. However, the conventional confocal microscope is used for optical signal imaging, and due to the limitation of diffraction limit of common optical signals, the resolution of the imaging is greatly limited.
Disclosure of Invention
The invention aims to provide a compressed light confocal detection device and a method, which are used for solving the problem of low imaging resolution when a sample is detected by using a common optical signal in the prior art.
In order to achieve the purpose, the invention provides the following scheme:
a compressed confocal optical detection apparatus comprising: the device comprises a compressed light emission source and a confocal detection module;
the compressed light emission source is connected with the confocal detection module and used for generating compressed light and enabling the compressed light to enter the confocal detection module; the confocal detection module is used for imaging a sample by using the compressed light.
Optionally, the compressed light emission source includes a laser source, a first polarization beam splitter, a nonlinear medium, a second polarization beam splitter, an optical parametric oscillator, and a third polarization beam splitter, which are arranged in sequence;
the laser source is used for emitting laser and enters the first polarization beam splitter;
the laser beam is split by the first polarization beam splitter and enters the nonlinear medium;
the nonlinear medium and the laser interact to generate a second harmonic, and the second harmonic is split by the second polarization beam splitter and enters the optical parametric oscillator;
the optical parametric oscillator generates compressed light according to the second harmonic, and the compressed light enters the confocal detection module through the third polarization beam splitter.
Optionally, the confocal detection module includes a dimming module, a beam splitter, a detection module, and an imaging module;
the compressed light emitting source, the dimming light path and the beam splitter are sequentially arranged;
the compressed light is subjected to light modulation through the light modulation module to obtain a one-dimensional line focusing light beam, the one-dimensional line focusing light beam enters the beam splitter, and reference light and detection light are obtained after beam splitting of the beam splitter; the propagation direction of the reference light is perpendicular to the propagation direction of the probe light;
the detection light enters the detection module to detect the sample, the detection light interacts with the sample, atoms in the sample absorb the detection light and then jump between atomic energy levels to emit fluorescence, the fluorescence carries sample information, the fluorescence returns along a light path of the detection light to the sample and undergoes Michelson interference in the returning process to obtain interfered fluorescence, and the interfered fluorescence is emitted into the imaging module through the beam splitter;
the imaging module images according to the fluorescence after interference and the reference light.
Optionally, the dimming module includes a first filter, a half-wave plate, an electro-optic modulator, a first beam expander, a second beam expander, a spatial polarizer, a polarization analyzer, and a cylindrical mirror, which are sequentially arranged;
the compressed light sequentially enters the first filter, the half-wave plate, the electro-optic modulator, the first beam expander, the second beam expander, the spatial polarizer, the polarization analyzer and the cylindrical mirror to obtain the one-dimensional line focusing light beam;
the first filter is used for screening out working wavelength compressed light from the compressed light;
the half-wave plate is used for polarizing the working wavelength compressed light to obtain polarized compressed light and enabling the polarized compressed light to enter the electro-optic modulator;
the electro-optic modulator is used for carrying out pulse modulation with set repetition frequency on the polarized compressed light to obtain modulated compressed light;
the first beam expander is used for expanding the modulated compressed light;
the second beam expander is used for expanding the expanded modulated compressed light again;
the spatial polaroid is used for adjusting the polarization direction of the modulated compressed light after twice beam expansion;
the polarization analyzer is used for positioning the polarization direction of the modulated compressed light after adjustment;
the cylindrical mirror is used for integrating the modulated and compressed light after being adjusted into the one-dimensional line focusing light beam and forming a line focus.
Optionally, the imaging module comprises: the second lens is arranged on the second excitation/emission galvanometer; the slit is placed on a focal plane of the fourth lens;
the reference light sequentially enters the fifth lens, the fourth lens, the slit, the third lens and the second filter to reach the second excitation/emission galvanometer, and is reflected by the second excitation/emission galvanometer and then reaches the receiver through the second lens for imaging; the second filter is used for filtering stray light.
Optionally, the detection module comprises: the device comprises a first lens, a first excitation/emission galvanometer, a scanning mirror, a tube mirror, an objective lens, a sample container, a first reflecting mirror and a second reflecting mirror; the sample container is placed on the focal plane of the objective lens;
the detection light reaches the first excitation/emission galvanometer after passing through the first lens, is reflected by the first excitation/emission galvanometer, then sequentially passes through the scanning lens, the tube lens and the objective lens to be emitted to the sample container, and is transmitted by the sample container to obtain transmission light, and the transmission light sequentially passes through the first reflector and the second reflector and then reaches the first excitation/emission galvanometer;
the detection light reaches the first excitation/emission galvanometer after passing through the first lens, and interacts with the sample when being reflected by the first excitation/emission galvanometer and then sequentially emitted to the sample in the sample container through the scanning lens, the tube lens and the objective lens, so that atoms in the sample absorb the detection light and then jump among atomic energy levels to emit fluorescence, and the fluorescence carries sample information; when the fluorescence returns to the first excitation/emission vibrating mirror along the optical path of the probe light to the sample, the fluorescence and the transmitted light reaching the first excitation/emission vibrating mirror generate Michelson interference to generate the interfered fluorescence; and the interfered fluorescence is emitted into the imaging module through the beam splitter to reach the receiver, and the receiver images according to the received interfered fluorescence and the reference light.
Optionally, the laser source is a solid laser, and the wavelength of the emitted laser is 1064 nm.
Optionally, when the receiver is a camera, the camera is used for direct imaging; when the receiver is a spectrometer, the spectrometer is used to analyse the composition of the sample.
A compressed light confocal detection method is applied to the compressed light confocal detection device and comprises the following steps:
generating a compressed light by using a compressed light emitting source;
inputting the compressed light into a confocal detection module;
and detecting and imaging the sample by the confocal detection module by using the compressed light.
Optionally, the compressed light emission source includes a laser source, a first polarization beam splitter, a nonlinear medium, a second polarization beam splitter, an optical parametric oscillator, and a third polarization beam splitter, which are arranged in sequence;
the laser source is used for emitting laser and enters the first polarization beam splitter;
the laser beam is split by the first polarization beam splitter and enters the nonlinear medium;
the nonlinear medium and the laser interact to generate a second harmonic, and the second harmonic is split by the second polarization beam splitter and enters the optical parametric oscillator;
the optical parametric oscillator generates compressed light according to the second harmonic, and the compressed light enters the confocal detection module through the third polarization beam splitter.
According to the specific embodiment provided by the invention, the invention discloses the following technical effects:
the invention discloses a compressed light confocal detection device and a method, wherein compressed light generated by a compressed light emission source enters a confocal detection module to detect a sample, and the compressed light can break through the diffraction limit, so that the resolution of sample imaging is greatly improved by detecting the sample through the compressed light.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without inventive exercise.
FIG. 1 is a schematic diagram of a compressed confocal optical detection apparatus according to the present invention;
FIG. 2 is a schematic diagram of a compact light source according to the present invention;
FIG. 3 is a structural diagram of a confocal detection module provided by the present invention.
Description of the symbols: the device comprises a compressed light emitting source 1, a first filter 2, a half-wave plate 3, an electro-optical modulator 4, a first beam expander 5, a second beam expander 6, a spatial polarizer 7, a polarization analyzer 8, a cylindrical mirror 9, a beam splitter 10, a first lens 11, a first excitation/emission galvanometer 12, a scanning mirror 13, a tube mirror 14, an objective lens 15, a sample container 16, a first reflecting mirror 17, a second reflecting mirror 18, a second excitation/emission galvanometer 19, a second lens 20, a receiver 21, a second filter 22, a third lens 23, a slit 24, a fourth lens 25, a fifth lens 26, a confocal detection module 27, a dimming module 28, an imaging module 29, a detection module 30, a laser source 101, a first polarization beam splitter 102, a nonlinear medium 103, a second polarization beam splitter 104, an optical parametric oscillator 105 and a third polarization beam splitter 106.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention aims to provide a compressed light confocal detection device and a method, which are used for solving the problem of low imaging resolution when a sample is detected by using a common optical signal in the prior art.
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in further detail below.
As shown in fig. 1, a compressed confocal optical detection apparatus includes: a compressed light emission source 1 and a confocal detection module 27; the compressed light emitting source 1 is connected with the confocal detection module 27, and the compressed light emitting source 1 is used for generating compressed light and entering the confocal detection module 27; the confocal detection module 27 is configured to image the sample with the compressed light.
As shown in fig. 2, as an optional implementation manner of this embodiment, the compressed light emitting source 1 includes a laser source 101, a first polarization beam splitter 102, a nonlinear medium 103, a second polarization beam splitter 104, an optical parametric oscillator 105, and a third polarization beam splitter 106, which are arranged in sequence.
The laser source 101 is used for emitting laser light and enters the first polarization beam splitter 102; the laser beam is split by the first polarization beam splitter 102 and enters the nonlinear medium 103; the nonlinear medium 103 interacts with the laser to generate a second harmonic, and the second harmonic is split by the second polarization beam splitter 104 and enters the optical parametric oscillator 105; the optical parametric oscillator 105 generates a compressed light according to the second harmonic, and the compressed light enters the confocal detection module 27 through the third polarization beam splitter 106.
As an optional implementation manner of this embodiment, as shown in fig. 1, the confocal detection module 27 includes a dimming module 28, a beam splitter 10, a detection module 30, and an imaging module 29.
The source 1, the dimming module 28 and the beam splitter 10 are arranged in sequence.
The compressed light is dimmed by the dimming module 28 to obtain a one-dimensional line focusing light beam, the one-dimensional line focusing light beam enters the beam splitter 10, and the reference light and the detection light are obtained after beam splitting by the beam splitter 10; the propagation direction of the reference light is perpendicular to the propagation direction of the probe light.
The detection light enters the detection module 30 to detect the sample, the detection light interacts with the sample, atoms in the sample absorb the detection light and then transition between atomic levels to emit fluorescence, the fluorescence carries sample information, the fluorescence returns along a light path of the detection light to the sample and undergoes michelson interference in the return process to obtain interfered fluorescence, and the interfered fluorescence is emitted into the imaging module 29 through the beam splitter 10; the imaging module 29 images according to the interfered fluorescence and the reference light.
As shown in fig. 3, as an optional implementation manner of this embodiment, the dimming module 28 includes a first filter 2, a half-wave plate 3, an electro-optical modulator 4, a first beam expander 5, a second beam expander 6, a spatial polarizer 7, a polarization analyzer 8, and a cylindrical mirror 9, which are arranged in sequence.
The compressed light sequentially enters the first filter 2, the half-wave plate 3, the electro-optic modulator 4, the first beam expander 5, the second beam expander 6, the spatial polarizer 7, the polarization analyzer 8 and the cylindrical mirror 9 to obtain the one-dimensional line focusing light beam.
The first filter 2 is used for screening out the working wavelength compressed light from the compressed light; the half-wave plate 3 is used for polarizing the working wavelength compressed light to obtain polarized compressed light and enabling the polarized compressed light to enter the electro-optic modulator 4; the electro-optical modulator 4 is configured to perform pulse modulation with a set repetition frequency on the polarized compressed light to obtain modulated compressed light.
The first beam expander 5 is used for expanding the modulated compressed light; the second beam expander 6 is used for expanding the modulated and compressed light after expanding the beam again; the spatial polaroid 7 is used for adjusting the polarization direction of the modulated compressed light after twice beam expansion; the polarization analyzer 8 is used for positioning the polarization direction of the modulated compressed light after adjustment; adjusting a polarization direction according to the positioning of the polarization analyzer; the cylindrical mirror 9 is used for integrating the modulated and compressed light after adjustment into the one-dimensional line focusing light beam and forming a line focus.
As an optional implementation manner of this embodiment, the imaging module 29 includes: a fifth lens 26, a fourth lens 25, a slit 24, a third lens 23, a second filter 22, a second excitation/emission galvanometer 19, a second lens 20, and a receiver 21; the slit 24 is placed on the focal plane of the fourth lens 25; the size of the slit 24 determines the depth, resolution and imaging quality at which the probed sample can be detected.
The reference light sequentially enters the fifth lens 26, the fourth lens 25, the slit 24, the third lens 23 and the second filter 22 to reach the second excitation/emission galvanometer 19, and is reflected by the second excitation/emission galvanometer 19 and then reaches the receiver 21 through the second lens 20 to form an image; the second filter 22 is used to filter out stray light.
As an optional implementation manner of this embodiment, the detection module 30 includes: a first lens 11, a first excitation/emission galvanometer 12, a scanning mirror 13, a tube mirror 14, an objective lens 15, a sample container 16, a first reflecting mirror 17 and a second reflecting mirror 18; the sample container 16 is placed in the focal plane of the objective 15.
The probe light reaches the first excitation/emission galvanometer 12 after passing through the first lens 11, is reflected by the first excitation/emission galvanometer 12, then sequentially passes through the scanning lens, the tube lens 14 and the objective lens 15 to irradiate towards the sample container 16, and is transmitted by the sample container 16 to obtain transmitted light, and the transmitted light sequentially passes through the first reflector 17 and the second reflector 18 to reach the first excitation/emission galvanometer 12 after being reflected.
The probe light reaches the first excitation/emission galvanometer 12 after passing through the first lens 11, and when the probe light is reflected by the first excitation/emission galvanometer 12 and then sequentially passes through the scanning lens, the tube lens 14 and the objective lens 15 to be emitted to a sample in the sample container 16, the probe light interacts with the sample, so that atoms in the sample absorb the probe light and then jump between atomic energy levels to emit fluorescence, and the fluorescence carries sample information; when the fluorescence returns to the first excitation/emission galvanometer 12 along the optical path of the probe light toward the sample, michelson interference occurs between the fluorescence and the transmitted light reaching the first excitation/emission galvanometer 12, and the interfered fluorescence is generated; the interfered fluorescence is emitted into the imaging module 29 through the beam splitter 10 to reach the receiver 21, and the receiver 21 images according to the received interfered fluorescence and the reference light.
As an optional implementation manner of this embodiment, the laser source 101 is a solid laser, and the wavelength of the emitted laser light is 1064 nm.
As an optional implementation manner of this embodiment, when the receiver 21 is a camera, the camera is used for direct imaging; when the receiver 21 is a spectrometer, the spectrometer is used to analyse the composition of the sample.
A compressed light confocal detection method is applied to the compressed light confocal detection device and comprises the following steps:
generating a compressed light by using the compressed light emitting source 1; inputting the compressed light into a confocal detection module 27; the compressed light is used to perform detection imaging on the sample by the confocal detection module 27.
As an optional implementation manner of this embodiment, the compressed light emitting source 1 includes a laser source 101, a first polarization beam splitter 102, a nonlinear medium 103, a second polarization beam splitter 104, an optical parametric oscillator 105, and a third polarization beam splitter 106, which are sequentially arranged.
The laser source 101 is used for emitting laser light and enters the first polarization beam splitter 102; the laser beam is split by the first polarization beam splitter 102 and enters the nonlinear medium 103; the nonlinear medium 103 interacts with the laser to generate a second harmonic, and the second harmonic is split by the second polarization beam splitter 104 and enters the optical parametric oscillator 105; the optical parametric oscillator 105 generates a compressed light according to the second harmonic, and the compressed light enters the confocal detection module 27 through the third polarization beam splitter 106.
The invention relates to a photon carrying detection information and the focusing depth of sample test, which determines the depth and reaction mechanism of the tested sample, compared with the conventional microscope, the invention has the advantages that noise and scattered photons are distinguished by low momentum transfer, the confocal microscope effectively reduces the interference of non-focal plane noise, improves the spatial resolution and the imaging quality, but the confocal microscope still utilizes common signal light for detection, the common signal still has great restriction on the imaging resolution due to the limitation of diffraction limit, the compressed light breaks through the diffraction limit, the imaging resolution can be greatly improved by utilizing the compressed light for detection, but the prior art does not have a concrete device for compressing the light imaging confocal, and can not realize the research of ultrahigh-precision sample imaging confocal detection and target system mechanism and quantum nonlinear characteristics based on the concrete device, the invention provides a compressed light confocal detection device, the compressed light is combined with the confocal light, the compressed light is generated by the compressed light emitting source to detect the sample, the resolution of the sample imaging can be greatly improved as the compressed light breaks through the diffraction limit, and meanwhile, the confocal light is adopted to effectively reduce the interference of non-focal plane noise and improve the imaging quality.
The mechanism of compressive light generation:
and obtaining an intensity difference compression state by using a II-type noncritical phase matching parametric oscillation cavity. The transmission loss has a large influence on the degree of compression in the compressed state and is very sensitive. The intensity difference compressed light obtained in an experiment only needs to operate the optical parameter cavity above a threshold value, the intensity correlation characteristic exists between the signal light and the idle light, and the noise of the signal light is counteracted by measuring the noise of the idle light, so that the noise of the signal light is reduced to a state of poisson distribution. The compressed state light field is one that compresses the fluctuations of one of the conjugate variables of the light wave field below the corresponding vacuum fluctuations while the other field variable increases.
The following describes the compressed confocal optical detection apparatus provided by the present invention with reference to fig. 2 and 3:
the invention provides a compressed light confocal detection device, which specifically comprises: the device comprises a compressed light emitting source 1, a first filter 2, a half-wave plate 3, an electro-optical modulator 4, a first beam expander 5, a second beam expander 6, a spatial polarizer 7, a polarization analyzer 8, a cylindrical mirror 9, a beam splitter 10, a first lens 11, a first excitation/emission galvanometer 12, a scanning mirror 13, a tube mirror 14, an objective lens 15, a sample container 16, a first reflecting mirror 17, a second reflecting mirror 18, a first excitation/emission galvanometer 19, a second lens 20, a receiver 21, a second filter 22, a third lens 23, a slit 24, a fourth lens 25 and a fifth lens 26.
The compressed light emission source 1 includes a laser light source 101, a first polarization beam splitter 102, a nonlinear medium 103, a second polarization beam splitter 104, an optical parametric oscillator 105, and a third polarization beam splitter 106. The laser source 101 is followed by a first polarizing beam splitter 102, the first polarizing beam splitter 102 is followed by a non-linear medium 103, a second polarizing beam splitter 104 is located after the non-linear medium 103, the second polarizing beam splitter 104 is followed by an optical parametric oscillator 105, and the optical parametric oscillator 105 is followed by a third polarizing beam splitter 106.
The compressed light emission source 1 is a compressed light source which is generated after a secondary wave is generated by the interaction of a nonlinear medium and coherent laser and then passes through an optical parametric oscillator; the laser source 101 emits laser light to enter the first polarization beam splitter 102, the first polarization beam splitter 102 divides the laser light into two beams, one beam enters the nonlinear medium 103, the other beam is perpendicular to the front beam, the nonlinear medium 103 generates second harmonic, the second harmonic light is divided by the second polarization beam splitter 104 to enter the optical parametric oscillator 105, and the optical parametric oscillator 105 generates compressed light which is emitted by the third polarization beam splitter 106.
The first filter 2 receives a light beam emitted by a compressed light emission source 1, the half-wave plate 3 is positioned behind the first filter 2, and an electro-optical modulator 4, a first beam expander 5, a second beam expander 6, a spatial polarizer 7, a polarization analyzer 8, a cylindrical mirror 9 and a beam splitter 10 are sequentially arranged along the light propagation direction of the half-wave plate 3, the beam splitter 10 divides the propagated light into two beams of light, one beam of light is still propagated along the original line direction, the other beam of light is propagated perpendicularly to the original propagation direction, the first lens 11 is positioned behind the beam splitter 10, namely along the original light propagation direction, the first excitation/emission galvanometer 12 is placed behind the first lens 11, and the scanning mirror 13, the tube mirror 14, the objective lens 15 and the sample container 16 are sequentially placed on the light path of the propagated light of the first excitation/emission galvanometer 12; on the beam splitting path of the beam splitter 10, which propagates perpendicularly to the original propagation direction, a fifth lens 26, a fourth lens 25, a slit 24, a third lens 23, a second filter 22, a first excitation/emission galvanometer 19, a second lens 20, and a receiver 21 are sequentially arranged.
The first filter 2 receives the light beam emitted by the compressed light emission source 1 and then selects the working wavelength, the half-wave plate 3 polarizes the light beam with the working wavelength, the polarized light beam with the working wavelength is transmitted to the electro-optical modulator 4, the electro-optical modulator 4 performs pulse modulation with the required repetition frequency on the light beam with the working wavelength, the first beam expander 5 and the second beam expander 6 expand the light beam to expand the space for illuminating a sample, the space polarizer 7 further adjusts the polarization direction of the light beam, the polarization analyzer 8 accurately positions the polarization direction property of the polarized light, the cylindrical mirror 9 focuses the light beam into a one-dimensional line focusing light beam and forms a line focus, the first excitation/emission vibrating mirror 12 emits to the objective lens 15 through the scanning lens 13 and the tube lens 14, the sample container 6 is positioned on the focal plane of the objective lens 15, the scanning lens 13 sequentially performs line-by-line scanning to detect the sample in the sample container 16, at the moment, the sample container is transparent, the sample is excited by the exciting light, the exciting light excites electrons in the sample to an excited state, the electrons return to a ground state to emit fluorescence, the detected fluorescence returns along an exciting light path and enters a slit when encountering a beam splitter 10, the beam splitter 10 reflects the returned fluorescence to the slit 24, the slit is placed at a focal plane of the emitted light, and the size of the slit determines the detection depth, resolution and imaging quality of the sample, so that important information is imaged at a required spatial position.
The first reflector 17 reflects the transmitted detection light, the second reflector 18 reflects the transmitted light back to the first excitation/emission vibrating mirror 12, at this time, a michelson interference mode is generated, meanwhile, fluorescence generated by sample excitation is richer, the fluorescence returns through a light path to meet a slit, stray light generated on a non-focal surface, diffraction light and other information are blocked by the slit and cannot enter a receiver, and only light of a focal plane carrying sample information enters the receiver.
The beam splitter 10 splits the beam path which propagates perpendicular to the original propagation direction and emits to a fifth lens 26, a fourth lens 25, a slit 24, a third lens 23 and a second filter 22 to filter stray light such as scattered light and diffracted light, the light carrying the detection information of the sample enters a receiver 21 through a first excitation/emission vibrating mirror 19 and a second lens 20, the receiver 21 can directly image when being a camera, and the receiver can analyze the components of the sample when being a spectrometer.
The invention provides a specific experimental numerical description for a compressed light confocal detection device, which comprises the following specific steps:
the laser emission source is a solid laser and has an emission wavelength of 1064 nm.
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other.
The principles and embodiments of the present invention have been described herein using specific examples, which are provided only to help understand the method and the core concept of the present invention; meanwhile, for a person skilled in the art, according to the idea of the present invention, the specific embodiments and the application range may be changed. In view of the above, the present disclosure should not be construed as limiting the invention.
Claims (10)
1. A compressed confocal optical detection device, comprising: the device comprises a compressed light emission source and a confocal detection module;
the compressed light emission source is connected with the confocal detection module and used for generating compressed light and enabling the compressed light to enter the confocal detection module; the confocal detection module is used for imaging a sample by using the compressed light.
2. The confocal compressed light detection device according to claim 1, wherein the compressed light emission source comprises a laser source, a first polarization beam splitter, a nonlinear medium, a second polarization beam splitter, an optical parametric oscillator, and a third polarization beam splitter arranged in sequence;
the laser source is used for emitting laser and enters the first polarization beam splitter;
the laser beam is split by the first polarization beam splitter and enters the nonlinear medium;
the nonlinear medium and the laser interact to generate a second harmonic, and the second harmonic is split by the second polarization beam splitter and enters the optical parametric oscillator;
the optical parametric oscillator generates compressed light according to the second harmonic, and the compressed light enters the confocal detection module through the third polarization beam splitter.
3. The compressed confocal optical detection device of claim 2, wherein the confocal detection module comprises a dimming module, a beam splitter, a detection module, and an imaging module;
the compressed light emitting source, the dimming light path and the beam splitter are sequentially arranged;
the compressed light is subjected to light modulation through the light modulation module to obtain a one-dimensional line focusing light beam, the one-dimensional line focusing light beam enters the beam splitter, and reference light and detection light are obtained after beam splitting of the beam splitter; the propagation direction of the reference light is perpendicular to the propagation direction of the probe light;
the detection light enters the detection module to detect the sample, the detection light interacts with the sample, atoms in the sample absorb the detection light and then jump between atomic energy levels to emit fluorescence, the fluorescence carries sample information, the fluorescence returns along a light path of the detection light to the sample and undergoes Michelson interference in the returning process to obtain interfered fluorescence, and the interfered fluorescence is emitted into the imaging module through the beam splitter; the imaging module images according to the fluorescence after interference and the reference light.
4. The confocal detection device of claim 3, wherein the dimming module comprises a first filter, a half-wave plate, an electro-optic modulator, a first beam expander, a second beam expander, a spatial polarizer, a polarization analyzer, and a cylindrical mirror, which are arranged in sequence;
the compressed light sequentially enters the first filter, the half-wave plate, the electro-optic modulator, the first beam expander, the second beam expander, the spatial polarizer, the polarization analyzer and the cylindrical mirror to obtain the one-dimensional line focusing light beam;
the first filter is used for screening out working wavelength compressed light from the compressed light;
the half-wave plate is used for polarizing the working wavelength compressed light to obtain polarized compressed light and enabling the polarized compressed light to enter the electro-optic modulator; the electro-optic modulator is used for carrying out pulse modulation with set repetition frequency on the polarized compressed light to obtain modulated compressed light;
the first beam expander is used for expanding the modulated compressed light; the second beam expander is used for expanding the expanded modulated compressed light again; the spatial polaroid is used for adjusting the polarization direction of the modulated compressed light after twice beam expansion; the polarization analyzer is used for positioning the polarization direction of the modulated compressed light after adjustment; the cylindrical mirror is used for integrating the modulated and compressed light after being adjusted into the one-dimensional line focusing light beam and forming a line focus.
5. The confocal compressed light detection device of claim 4, wherein the imaging module comprises: the second lens is arranged on the second excitation/emission galvanometer; the slit is placed on a focal plane of the fourth lens;
the reference light sequentially enters the fifth lens, the fourth lens, the slit, the third lens and the second filter to reach the second excitation/emission galvanometer, and is reflected by the second excitation/emission galvanometer and then reaches the receiver through the second lens for imaging; the second filter is used for filtering stray light.
6. The confocal compressed light detection device of claim 5, wherein the detection module comprises: the device comprises a first lens, a first excitation/emission galvanometer, a scanning mirror, a tube mirror, an objective lens, a sample container, a first reflecting mirror and a second reflecting mirror; the sample container is placed on the focal plane of the objective lens;
the detection light reaches the first excitation/emission galvanometer after passing through the first lens, is reflected by the first excitation/emission galvanometer, then sequentially passes through the scanning lens, the tube lens and the objective lens to be emitted to the sample container, and is transmitted by the sample container to obtain transmission light, and the transmission light sequentially passes through the first reflector and the second reflector and then reaches the first excitation/emission galvanometer;
the detection light reaches the first excitation/emission galvanometer after passing through the first lens, and interacts with the sample when being reflected by the first excitation/emission galvanometer and then sequentially emitted to the sample in the sample container through the scanning lens, the tube lens and the objective lens, so that atoms in the sample absorb the detection light and then jump among atomic energy levels to emit fluorescence, and the fluorescence carries sample information; when the fluorescence returns to the first excitation/emission vibrating mirror along the optical path of the probe light to the sample, the fluorescence and the transmitted light reaching the first excitation/emission vibrating mirror generate Michelson interference to generate the interfered fluorescence; and the interfered fluorescence is emitted into the imaging module through the beam splitter to reach the receiver, and the receiver images according to the received interfered fluorescence and the reference light.
7. The confocal compressive optical detection device of claim 6, wherein the laser source is a solid-state laser emitting laser light having a wavelength of 1064 nm.
8. The confocal compressive optical detection device of claim 7,
when the receiver is a camera, the camera is used for direct imaging; when the receiver is a spectrometer, the spectrometer is used to analyse the composition of the sample.
9. A compressed confocal optical detection method, wherein the compressed confocal optical detection method is applied to the compressed confocal optical detection device according to any one of claims 1 to 8, and the compressed confocal optical detection method comprises:
generating a compressed light by using a compressed light emitting source; inputting the compressed light into a confocal detection module; and detecting and imaging the sample by the confocal detection module by using the compressed light.
10. The confocal compressed light detection method according to claim 9, wherein the compressed light emission source comprises a laser source, a first polarization beam splitter, a nonlinear medium, a second polarization beam splitter, an optical parametric oscillator, and a third polarization beam splitter arranged in sequence;
the laser source is used for emitting laser and enters the first polarization beam splitter;
the laser beam is split by the first polarization beam splitter and enters the nonlinear medium;
the nonlinear medium and the laser interact to generate a second harmonic, and the second harmonic is split by the second polarization beam splitter and enters the optical parametric oscillator;
the optical parametric oscillator generates compressed light according to the second harmonic, and the compressed light enters the confocal detection module through the third polarization beam splitter.
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