CN113049308A - Tumor cell sampling device - Google Patents
Tumor cell sampling device Download PDFInfo
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- CN113049308A CN113049308A CN202110259682.8A CN202110259682A CN113049308A CN 113049308 A CN113049308 A CN 113049308A CN 202110259682 A CN202110259682 A CN 202110259682A CN 113049308 A CN113049308 A CN 113049308A
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- 238000005070 sampling Methods 0.000 title claims abstract description 144
- 210000004881 tumor cell Anatomy 0.000 title claims abstract description 33
- 238000009434 installation Methods 0.000 claims abstract description 17
- 230000007246 mechanism Effects 0.000 claims abstract description 8
- 206010028980 Neoplasm Diseases 0.000 claims description 29
- 238000000034 method Methods 0.000 claims description 7
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 4
- 210000001519 tissue Anatomy 0.000 description 15
- 210000004027 cell Anatomy 0.000 description 8
- 238000006243 chemical reaction Methods 0.000 description 6
- 201000011510 cancer Diseases 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 201000009030 Carcinoma Diseases 0.000 description 2
- 230000006835 compression Effects 0.000 description 2
- 238000007906 compression Methods 0.000 description 2
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 206010016654 Fibrosis Diseases 0.000 description 1
- 206010039491 Sarcoma Diseases 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000001574 biopsy Methods 0.000 description 1
- 230000002308 calcification Effects 0.000 description 1
- 208000035269 cancer or benign tumor Diseases 0.000 description 1
- 230000005779 cell damage Effects 0.000 description 1
- 208000037887 cell injury Diseases 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 208000035475 disorder Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 210000000981 epithelium Anatomy 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000004761 fibrosis Effects 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 231100000588 tumorigenic Toxicity 0.000 description 1
- 230000000381 tumorigenic effect Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N1/14—Suction devices, e.g. pumps; Ejector devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N1/14—Suction devices, e.g. pumps; Ejector devices
- G01N2001/1418—Depression, aspiration
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- Life Sciences & Earth Sciences (AREA)
- Hydrology & Water Resources (AREA)
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a tumor cell sampling device, which comprises a fixing mechanism, wherein a sampling device is arranged at the center of the top of the fixing mechanism, a connecting block is arranged at the bottom end of the sampling device, a sampling needle is fixedly arranged at the bottom end of the connecting block, a sleeve is slidably sleeved on the surface wall of the sampling needle close to the bottom, pressing plates are respectively arranged at the left end and the right end of the sleeve, a moving plate is fixedly connected with one end close to each of the two pressing plates, a fastening plate is fixedly connected with the top ends of the two moving plates, the sampling needle is arranged on the sampling device, the connecting block can push the clamping blocks at the two sides to respectively rotate towards the two sides during installation, so that the mounting springs at the two sides are compressed and deformed, after the connecting block is arranged in a mounting groove, the clamping blocks at the two sides can be pushed to be tightly attached, the device has realized the installation and the change of being convenient for to the sampling needle for sampling device only needs the disinfection can used repeatedly.
Description
Technical Field
The invention belongs to the technical field of medical instruments, and particularly relates to a tumor cell sampling device.
Background
Tumor refers to a new organism formed by local tissue cell proliferation under the action of various tumorigenic factors, because the new organism mostly presents an occupied block-shaped protrusion, also called a neoplasm, and the tumor is divided into two categories of benign tumor and malignant tumor according to the cell characteristics of the new organism and the degree of harmfulness to the organism. Malignant tumors can be divided into carcinoma and sarcoma, carcinoma refers to malignant tumor derived from epithelial tissue, with the development of medicine, before clinical treatment is performed on patients with tumors, tumor living bodies need to be examined, at present, a sample is generally extracted by a syringe through negative pressure suction in clinic, but when tumor biopsy sampling is performed, because tumors are in human cells, a sampling needle is difficult to sample at an accurate position, the sampling quality is influenced, in addition, some tumors can generate calcification or fibrosis in the development process, the hardness of the tumors is too high, multiple times of extraction is possibly required, the sampling difficulty and material waste are increased, even tumor cell sampling failure can be caused, and the sampling efficiency is influenced.
Disclosure of Invention
The invention aims to: the sampling needle is arranged on the sampling device, the connecting block can push the clamping blocks at the two sides to respectively rotate towards the two sides during installation, so that the mounting springs at the two sides are extruded to compress and deform, after the connecting block is arranged in the mounting groove, the clamping blocks at the two sides can be pushed to cling to the two sides of the connecting block under the reaction force of the mounting spring, so that the sampling needle can be stably arranged on the sampling device, the device realizes convenient installation and replacement of the sampling needle, can ensure sanitation and can be reused only by disinfection, when tumor cells are collected, the tumor cells drive the internal anti-reverse plate to rotate when flowing through the drainage tube, so that the compression of the reset spring is extruded and the deformation is generated, after the tumor cells completely flow into the sampling bottle, the anti-reverse plate is driven to reset under the reaction force of the reset spring, so that the port of the drainage tube is sealed, and, make the device use can be more convenient, protect the sample needle through with the sleeve pipe, pass the press plate of both sides downwards, make its movable plate that drives both sides move down, thereby it moves down to drive two tie boards, when two tie boards move down, can gather together under the guide of locking groove, thereby it takes place deformation to extrude the compression of middle coupling spring, when two tie boards removed the locking inslot completely, can tighten up sheathed tube open-top department, make the sample needle can be kept properly in the use, avoid the sample needle to receive the pollution.
The technical scheme adopted by the invention is as follows: the utility model provides a tumor cell sampling device, includes fixed establishment, fixed establishment's top center department is provided with sampling device, sampling device's bottom is provided with the connecting block, the bottom fixed mounting of connecting block has the sampling needle, the table wall of sampling needle is close to bottom slip cap and is equipped with the sleeve pipe, both ends all are provided with press the clamp plate about the sheathed tube, two the equal fixedly connected with movable plate of one end that press the clamp plate is close, and the equal fixedly connected with in top of two movable plates tie the board, two tie two coupling springs of fixedly connected with between the board, sampling device's rear end is provided with the drainage tube, the top fixed mounting of drainage tube has the sampling bottle, sampling device's top is provided with the pull rod, the bottom fixedly connected with piston of pull rod.
The fixing mechanism comprises a fixing plate, a placing groove is formed in the upper surface of the fixing plate, the interior of the placing groove is connected with the sampling device in a sliding mode, a supporting rod is fixedly connected to the bottom end of the fixing plate, and a base is fixedly connected to the bottom end of the supporting rod.
Wherein, the negative pressure hole has been seted up on sampling device's top, the inner wall and the piston sliding connection in negative pressure hole, the mounting groove has been seted up to sampling device's bottom, the inside left and right sides of mounting groove all rotates and is connected with the fixture block, and the equal fixedly connected with installation spring of the one end that two fixture blocks leave mutually, two the one end that the installation spring leaves mutually all with mounting groove fixed connection, the drainage hole has been seted up to sampling device's rear end, the inside and the drainage tube sliding connection in drainage hole.
The inner walls of the two ends of the drainage tube are rotatably connected with anti-reverse plates, the left ends of the anti-reverse plates are fixedly connected with return springs, and the left ends of the two return springs are fixedly connected to the inner walls of the two ends of the drainage tube respectively.
The locking groove is formed in the top end of the sleeve, the sliding grooves are formed in the left end and the right end of the sleeve, the inner walls of the two sliding grooves are connected with the two moving plates in a sliding mode respectively, and the locking groove is formed in the top end of each moving plate in a penetrating mode.
The middle position of the inside of the mounting groove is connected with the connecting block in a sliding mode, and the two clamping blocks are located at two ends of the connecting block respectively.
Wherein, the inside left and right sides of mounting groove all is connected with the fixture block through the bearing rotation, and the equal fixedly connected with wear pad of one end that two fixture blocks are close.
Wherein, all be connected with anti-contrary board through the pivot rotation on the both ends inner wall of drainage tube, two reset spring's surface all overlaps and is equipped with sealed the pad.
Wherein, the outer wall of sample bottle is carved with the scale groove, and the front surface fixed mounting of sample bottle has the observation window.
A method of using a tumor cell sampling device, comprising the steps of:
s1: assembling the device: put into the mounting groove with the connecting block in, make it pass both sides fixture block and rotate, can remove the mounting groove up to the connecting block in, install the top at the drainage tube with the sample bottle again, install the drainage tube and stabilize in the drainage hole and place.
S2: extracting tumor cells: through pulling the pull rod, drive the piston and shift up in the downthehole portion of negative pressure, aim at the tumour cell with the sample, press the pull rod again, move the bottom in negative pressure hole until the piston, pull the pull rod again at last, make the tumour cell be extracted on the sampling needle.
S3: and (3) tumor cell storage: place sampling device slope, make during tumour cell can pack into the sampling bottle through the drainage tube, place sampling device on the standing groove with the sleeve pipe cover on the sampling needle again.
In summary, due to the adoption of the technical scheme, the invention has the beneficial effects that:
(1) according to the sampling device, the sampling needle is arranged on the sampling device, the connecting block can push the clamping blocks on the two sides to respectively rotate towards the two sides during installation, so that the installation springs extruding the two sides are compressed to deform, after the connecting block is arranged in the installation groove, the clamping blocks on the two sides can be pushed to cling to the two sides of the connecting block under the reaction force of the installation springs, the sampling needle can be stably arranged on the sampling device, the sampling needle can be conveniently installed and replaced by the sampling needle, and the sampling device can be repeatedly used only by disinfection.
(2) According to the invention, when tumor cells are collected and flow through the drainage tube, the tumor cells drive the internal anti-reverse plate to rotate, so that the reset spring is extruded to be compressed and deformed, and after the tumor cells completely flow into the sampling bottle, the anti-reverse plate is driven to reset under the counter-acting force of the reset spring, so that the port of the drainage tube is sealed, the tumor cells are prevented from flowing reversely, and the device is more convenient to use.
(3) According to the invention, the sampling needle is protected by the sleeve, the pressing plates on the two sides are pushed downwards to drive the movable plates on the two sides to move downwards, so that the two tightening plates are driven to move downwards, and when the two tightening plates move downwards, the two tightening plates are guided by the locking groove to converge, so that the connecting spring in the middle is extruded to be compressed and deformed, when the two tightening plates completely move into the locking groove, the opening at the top of the sleeve can be tightened, so that the sampling needle can be properly stored in the using process, and the sampling needle is prevented from being polluted.
Drawings
FIG. 1 is a front perspective view of the present invention;
FIG. 2 is a front perspective view of the fixation structure of the present invention;
FIG. 3 is a front perspective view of a sampling device of the present invention;
FIG. 4 is an exploded bottom perspective view of the sampling device of the present invention;
FIG. 5 is a front cross-sectional view of a sampling device of the present invention;
FIG. 6 is an enlarged view taken at A of FIG. 5 in accordance with the present invention;
FIG. 7 is a front perspective view of the connector block of the present invention;
fig. 8 is a front perspective exploded view of the bushing of the present invention.
The labels in the figure are: 1. a fixing mechanism; 101. a placement groove; 102. a fixing plate; 103. a support bar; 104. a base; 2. a drainage tube; 3. sampling a bottle; 4. a pull rod; 5. a sampling device; 501. a negative pressure hole; 502. mounting grooves; 503. a clamping block; 504. installing a spring; 505. a drainage hole; 6. a sleeve; 601. a chute; 602. a locking groove; 7. a sampling needle; 8. a pressing plate; 9. connecting blocks; 10. moving the plate; 11. a bolting plate; 12. a connecting spring; 13. a piston; 14. a check plate; 15. a return spring.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Example one, see fig. 1-5: a tumor cell sampling device comprises a fixing mechanism 1, a sampling device 5 is arranged at the center of the top of the fixing mechanism 1, the sampling device 5 is used for extracting and storing tumor cells, a connecting block 9 is arranged at the bottom end of the sampling device 5, a sampling needle 7 is fixedly arranged at the bottom end of the connecting block 9, the connecting block 9 is used for conveniently installing the sampling needle 7 on the sampling device 5, meanwhile, the connecting block 9 can be communicated with the sampling device 5 and the sampling needle 7, cells extracted from the sampling needle 7 can flow into the sampling device 5, a sleeve 6 is slidably sleeved on the surface wall of the sampling needle 7 close to the bottom, the sleeve 6 is sleeved on the sampling needle 7, on one hand, the sampling needle 7 is protected, on the other hand, the sampling needle 7 can be prevented from being polluted, pressing plates 8 are arranged at the left end and the right end of the sleeve 6, one end close to the two pressing, the top ends of the two moving plates 10 are fixedly connected with tightening plates 11, two connecting springs 12 are fixedly connected between the two tightening plates 11, the top of the sleeve 6 is loose, the sleeve 6 is sleeved on the sampling needle 7 and then pushed downwards to push the pressing plates 8 at two sides, so that the moving plates 10 at two sides are driven to move downwards, the two tightening plates 11 are driven to move downwards, the opening at the top of the sleeve 6 can be tightened, the sleeve can be used for protecting sampling needles 7 of different specifications, the rear end of the sampling device 5 is provided with a drainage tube 2, the drainage tube 2 is used for draining tumor tissues in the sampling device 5, the top end of the drainage tube 2 is fixedly provided with a sampling bottle 3, the sampling bottle 3 can properly store tumor cells and avoid cell damage, the top end of the sampling device 5 is provided with a pull rod 4, the bottom end of the pull rod 4 is fixedly connected with a piston 13, the piston 13 is used for controlling the sampling device 5 to blow away cells and, the fixing mechanism 1 comprises a fixing plate 102, a placing groove 101 is formed in the upper surface of the fixing plate 102, the interior of the placing groove 101 is slidably connected with a sampling device 5, the placing groove 101 is used for placing the sampling device 5, the sampling efficiency is low due to the fact that the sampling device 5 is placed in disorder is avoided, a supporting rod 103 is fixedly connected to the bottom end of the fixing plate 102, the length of the supporting rod 103 is adjustable, medical staff can conveniently adjust according to the size specification of a sampling needle 7, and meanwhile work of the medical staff is facilitated, a base 104 is fixedly connected to the bottom end of the supporting rod 103, a negative pressure hole 501 is formed in the top end of the sampling device 5, the inner wall of the negative pressure hole 501 is slidably connected with a piston 13, the piston 13 moves up and down in the negative pressure hole 501, negative pressure generated in the negative pressure hole 501 can be used for extracting cells, or high-pressure, so that the space between the connecting block 9 and the sampling device 5 can be sealed to avoid the tumor tissue from overflowing, the left side and the right side of the inside of the mounting groove 502 are rotatably connected with the clamping blocks 503, the separated ends of the two clamping blocks 503 are fixedly connected with the mounting springs 504, the separated ends of the two mounting springs 504 are fixedly connected with the mounting groove 502, the mounting and the dismounting of the connecting block 9 can be facilitated through the matching of the clamping blocks 503 and the mounting springs 504, the sampling device 5 can be repeatedly used for a plurality of times, only disinfection is needed, the sampling needle 7 is a disposable instrument, in order to ensure sanitation and safety, the rear end of the sampling device 5 is provided with the drainage hole 505, the inside of the drainage hole 505 is in sliding connection with the drainage tube 2, the pull rod 4 is pulled to drive the piston 13 to move upwards in the negative pressure hole 501, so that negative pressure is generated in the negative pressure hole 501, cells are conveniently pumped into the negative pressure, thereby flowing into the sampling bottle 3 for storage.
Example two, with reference to fig. 7-8: the inner walls of the two ends of the drainage tube 2 are rotatably connected with anti-reverse plates 14, the anti-reverse plates 14 are used for preventing cells flowing into the drainage tube 2 from reversely flowing back into the negative pressure hole 501 again, the left ends of the two anti-reverse plates 14 are fixedly connected with a reset spring 15, when the cells flow into the drainage tube 2, the two anti-reverse plates 14 are driven to rotate, so that the reset spring 15 is extruded to be compressed and deformed, after the cells flow into the drainage tube 2, the two anti-reverse plates 14 are driven to reset under the reaction force of the reset spring 15, so that the drainage tube 2 is blocked, the tumor tissue is prevented from flowing back, the left ends of the two reset springs 15 are respectively and fixedly connected to the inner walls of the two ends of the drainage tube 2, the top end of the sleeve 6 is provided with a locking groove 602, the left end and the right end of the sleeve 6 are respectively provided with sliding grooves 601, the inner walls of, when the two fastening plates 11 move downwards, the two fastening plates are converged under the guidance of the locking groove 602, so as to extrude the connecting spring 12 in the middle to be compressed and deformed, when the two fastening plates 11 completely move into the locking groove 602, the opening at the top of the sleeve 6 can be folded and fixed, so that the sleeve is not easy to fall off from the sampling needle 7, the middle position inside the mounting groove 502 is connected with the connecting block 9 in a sliding manner, the two fixture blocks 503 are respectively positioned at the two ends of the connecting block 9, when the connecting block 9 is mounted, the two fixture blocks 503 can be pushed to rotate towards the two sides, so as to extrude the mounting springs 504 at the two sides to be compressed and deformed, when the connecting block 9 moves into the mounting groove 502, the two fixture blocks 503 at the two sides can be pushed to be tightly attached to the two sides of the connecting block 9 under the reactive force of the mounting springs 504, so that the sampling needle 7 can be stably mounted on the, prevent contrary board 14 and install on drainage tube 2 through the pivot, make its rotation can be more smooth-going, avoid taking place the card stopper, two reset spring 15's surface all overlaps and is equipped with sealed the pad, and sealed pad can avoid tumour tissue and reset spring 15 contact, avoids tumour tissue to take place to deteriorate.
A method of using a tumor cell sampling device, comprising the steps of:
the method comprises the following steps: assembling the device: put into mounting groove 502 with connecting block 9 in, make it pass both sides fixture block 503 and rotate, until connecting block 9 can remove in mounting groove 502, install sampling bottle 3 on the top of drainage tube 2 again, install drainage tube 2 and stabilize in drainage hole 505 and place step two: extracting tumor tissues: through pulling pull rod 4, drive piston 13 shifts up in negative pressure hole 501 inside, aligns the tumour tissue with sampling needle 7, presses pull rod 4 again, moves the bottom in negative pressure hole 501 until piston 13, pull rod 4 again at last, makes tumour tissue be extracted to sampling needle 7 on, step three: and (3) tumor tissue storage: place sampling device 5 slope, make tumour tissue can pack into sampling bottle 3 through drainage tube 2 in, overlap sleeve pipe 6 again on sampling needle 7, place sampling device 5 on standing groove 101.
When in use, the medical staff installs the connecting block 9 in the installation groove 502 of the sampling device 5, the connecting block 9 pushes the fixture blocks 503 at two sides to rotate towards two sides respectively during installation, thereby compressing the installation springs 504 at two sides to generate deformation, after the connecting block 9 is installed in the installation groove 502, the fixture blocks 503 at two sides can be pushed to cling to two sides of the connecting block 9 under the reaction force of the installation springs 504, so that the sampling needle 7 can be stably installed on the sampling device 5, then the sampling bottle 3 is installed at the top end of the drainage tube 2, the drainage tube 2 is installed in the drainage hole 505 to be stably placed, then the pull rod 4 is pulled to drive the piston 13 to move upwards in the negative pressure hole 501, so that the negative pressure state in the negative pressure hole 501 is realized, the sampling needle 7 is aligned above the tumor tissue, the pull rod 4 is pressed, so that the sampling needle 7 can blow off the tumor cells firstly until the piston 13 moves, then the sampling needle 7 is placed on the tumor tissue, the pull rod 4 is pulled to draw the tumor tissue into the sampling needle 7, so as to be collected inside the negative pressure hole 501, the sampling device 5 is obliquely placed, so that the tumor tissue can be loaded into the sampling bottle 3 through the drainage tube 2, when the tumor cells flow through the drainage tube 2, the internal anti-reverse plate 14 is driven to rotate, so as to extrude the reset spring 15 to compress and deform, after the tumor cells completely flow into the sampling bottle 3, the anti-reverse plate 14 is driven to reset under the reaction force of the reset spring 15, so as to seal the port of the drainage tube 2, prevent the tumor cells from flowing backwards, then the sleeve 6 is sleeved on the sampling needle 7, the pressing plates 8 at the two sides are pushed downwards, so as to drive the moving plates 10 at the two sides to move downwards, so as to drive the two tightening plates 11 to move downwards, when the two tightening plates 11 move downwards, the sleeve is guided by the locking groove 602 to converge, so as to extrude the connecting spring 12, when the two bolting plates 11 are completely moved into the locking groove 602, the top opening of the sleeve 6 can be tightened, so that the sampling needle 7 is fixed to avoid being polluted in the using process, and finally the sampling device 5 is placed on the placing groove 101.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (10)
1. A tumor cell sampling device, includes fixed establishment (1), the top center department of fixed establishment (1) is provided with sampling device (5), its characterized in that: the bottom end of the sampling device (5) is provided with a connecting block (9), the bottom end of the connecting block (9) is fixedly provided with a sampling needle (7), a sleeve (6) is slidably sleeved on the surface wall of the sampling needle (7) close to the bottom, pressing plates (8) are arranged at the left end and the right end of the sleeve (6), a moving plate (10) is fixedly connected at one end close to each pressing plate (8), the top ends of the two moving plates (10) are fixedly connected with bolting plates (11), two connecting springs (12) are fixedly connected between the two bolting plates (11), a drainage tube (2) is arranged at the rear end of the sampling device (5), a sampling bottle (3) is fixedly arranged at the top end of the drainage tube (2), the top of sampling device (5) is provided with pull rod (4), the bottom fixedly connected with piston (13) of pull rod (4).
2. A tumor cell sampling device according to claim 1, wherein: the fixing mechanism (1) comprises a fixing plate (102), a placing groove (101) is formed in the upper surface of the fixing plate (102), the interior of the placing groove (101) is connected with the sampling device (5) in a sliding mode, a supporting rod (103) is fixedly connected to the bottom end of the fixing plate (102), and a base (104) is fixedly connected to the bottom end of the supporting rod (103).
3. A tumor cell sampling device according to claim 2, wherein: negative pressure hole (501) have been seted up on the top of sampling device (5), the inner wall and piston (13) sliding connection in negative pressure hole (501), mounting groove (502) have been seted up to the bottom of sampling device (5), the inside left and right sides of mounting groove (502) is all rotated and is connected with fixture block (503), and two equal fixedly connected with installation spring (504) of one end that fixture block (503) separated from each other, two the one end that installation spring (504) separated from each other all with mounting groove (502) fixed connection, drainage hole (505) have been seted up to the rear end of sampling device (5), the inside and drainage tube (2) sliding connection of drainage hole (505).
4. A tumor cell sampling device according to claim 3, wherein: the drainage tube is characterized in that anti-reverse plates (14) are rotatably connected to the inner walls of the two ends of the drainage tube (2), two return springs (15) are fixedly connected to the left ends of the anti-reverse plates (14), and the left ends of the two return springs (15) are fixedly connected to the inner walls of the two ends of the drainage tube (2) respectively.
5. The device according to claim 4, wherein: locking groove (602) have been seted up on the top of sleeve pipe (6), spout (601) have all been seted up at both ends about sleeve pipe (6), and the inner wall of two spout (601) respectively with two movable plate (10) sliding connection, two locking groove (602) all runs through on the top of movable plate (10).
6. The device according to claim 5, wherein: the middle position in the mounting groove (502) is connected with the connecting block (9) in a sliding mode, and the two clamping blocks (503) are located at two ends of the connecting block (9) respectively.
7. The device according to claim 6, wherein: the left side and the right side of the inside of the mounting groove (502) are rotatably connected with clamping blocks (503) through bearings, and one ends of the two clamping blocks (503) which are close to each other are fixedly connected with wear-resistant pads.
8. The device according to claim 7, wherein: all be connected with anti-contrary board (14), two through the pivot rotation on the both ends inner wall of drainage tube (2) the surface of reset spring (15) all is equipped with sealed the pad.
9. A tumor cell sampling device according to claim 8, wherein: the outer wall of sampling bottle (3) is carved with the scale groove, and the front surface fixed mounting of sampling bottle (3) has the observation window.
10. A method of using a tumor cell sampling device according to any one of claims 1 to 9, comprising the steps of:
s1: assembling the device: putting the connecting block (9) into the mounting groove (502), pushing the clamping blocks (503) on the two sides to rotate until the connecting block (9) can move into the mounting groove (502), then installing the sampling bottle (3) at the top end of the drainage tube (2), and installing the drainage tube (2) into the drainage hole (505) for stable placement;
s2: extracting tumor tissues: the pull rod (4) is pulled to drive the piston (13) to move upwards in the negative pressure hole (501), the sampling needle (7) is aligned to the tumor tissue, the pull rod (4) is pressed until the piston (13) moves to the bottom of the negative pressure hole (501), and finally the pull rod (4) is pulled to enable the tumor tissue to be extracted to the sampling needle (7);
s3: and (3) tumor tissue storage: the sampling device (5) is obliquely arranged, so that tumor tissues can be filled into the sampling bottle (3) through the drainage tube (2), the sleeve (6) is sleeved on the sampling needle (7), and the sampling device (5) is arranged on the placing groove (101).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110259682.8A CN113049308A (en) | 2021-03-10 | 2021-03-10 | Tumor cell sampling device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110259682.8A CN113049308A (en) | 2021-03-10 | 2021-03-10 | Tumor cell sampling device |
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| CN113049308A true CN113049308A (en) | 2021-06-29 |
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| CN202110259682.8A Pending CN113049308A (en) | 2021-03-10 | 2021-03-10 | Tumor cell sampling device |
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Application publication date: 20210629 |