CN113030031A - Oxygen concentration test method, oxygen concentration test device and fluorescent oxygen sensor - Google Patents
Oxygen concentration test method, oxygen concentration test device and fluorescent oxygen sensor Download PDFInfo
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- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 title claims abstract description 337
- 239000001301 oxygen Substances 0.000 title claims abstract description 337
- 229910052760 oxygen Inorganic materials 0.000 title claims abstract description 337
- 238000012360 testing method Methods 0.000 title claims abstract description 47
- 238000010998 test method Methods 0.000 title claims description 17
- 230000010363 phase shift Effects 0.000 claims abstract description 83
- 238000004364 calculation method Methods 0.000 claims abstract description 79
- 238000000034 method Methods 0.000 claims description 5
- 238000010791 quenching Methods 0.000 description 17
- 230000000171 quenching effect Effects 0.000 description 15
- 238000010586 diagram Methods 0.000 description 4
- 230000005283 ground state Effects 0.000 description 4
- 239000000126 substance Substances 0.000 description 3
- 230000004931 aggregating effect Effects 0.000 description 2
- 230000005284 excitation Effects 0.000 description 2
- 230000005281 excited state Effects 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 239000012456 homogeneous solution Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000007704 transition Effects 0.000 description 2
- VFMUXPQZKOKPOF-UHFFFAOYSA-N 2,3,7,8,12,13,17,18-octaethyl-21,23-dihydroporphyrin platinum Chemical compound [Pt].CCc1c(CC)c2cc3[nH]c(cc4nc(cc5[nH]c(cc1n2)c(CC)c5CC)c(CC)c4CC)c(CC)c3CC VFMUXPQZKOKPOF-UHFFFAOYSA-N 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
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- 238000007429 general method Methods 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
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- 230000004044 response Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6432—Quenching
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Abstract
The invention discloses an oxygen concentration testing method, an oxygen concentration testing device and a fluorescent oxygen sensor, wherein the oxygen concentration testing method comprises the following steps: acquiring a current phase shift value measured by a fluorescent oxygen sensor in a current oxygen concentration environment; acquiring a multi-site oxygen concentration calculation formula with known parameters; and calculating to obtain the current oxygen concentration in the current oxygen concentration environment according to the current phase shift value and a multi-site oxygen concentration calculation formula with known parameters. The oxygen concentration testing method provided by the embodiment of the invention can greatly improve the accuracy of the oxygen concentration test.
Description
Technical Field
The invention relates to the technical field of oxygen concentration detection, in particular to an oxygen concentration testing method, an oxygen concentration testing device and a fluorescent oxygen sensor.
Background
In the fluorescent oxygen sensor, a calculation formula of the oxygen concentration generally uses a Stern-Volmer equation to obtain a quenching constant through straight line fitting, so that an oxygen concentration calculation formula of the sensor can be obtained, and the oxygen concentration in the current oxygen concentration environment is calculated through the oxygen concentration calculation formula.
However, for many fluorescent oxygen sensors, quenching is accomplished in a heterogeneous medium, and the Stern-Volmer linear equation based on homogeneous solution physicochemical considerations is not necessarily appropriate, as is the case for solid-supported membrane sensors, and for deviations from the Stern-Volmer equation, such as nonlinear solution models and multi-site models.
Disclosure of Invention
The present invention is directed to solving, at least to some extent, one of the technical problems in the art described above.
Therefore, an object of the present invention is to provide an oxygen concentration test method capable of greatly improving the accuracy of the oxygen concentration test.
The second purpose of the invention is to provide an oxygen concentration testing device.
A third object of the present invention is to provide a fluorescent oxygen sensor.
In order to achieve the above object, an embodiment of the first aspect of the present invention provides an oxygen concentration testing method, including the following steps: acquiring a current phase shift value measured by a fluorescent oxygen sensor in a current oxygen concentration environment; acquiring a multi-site oxygen concentration calculation formula with known parameters; and calculating to obtain the current oxygen concentration in the current oxygen concentration environment according to the current phase shift value and a multi-site oxygen concentration calculation formula with known parameters.
According to the oxygen concentration testing method provided by the embodiment of the invention, the current phase shift value measured by the fluorescent oxygen sensor in the current oxygen concentration environment is firstly obtained, then the multi-site oxygen concentration calculation formula with known parameters is obtained, and finally the current oxygen concentration in the current oxygen concentration environment is obtained through calculation according to the current phase shift value and the multi-site oxygen concentration calculation formula with known parameters, so that the accuracy of the oxygen concentration test is greatly improved.
In addition, the oxygen concentration test method proposed according to the above embodiment of the present invention may also have the following additional technical features:
in one embodiment of the present invention, the multi-site oxygen concentration calculation formula with known acquisition parameters comprises: acquiring a first phase shift value measured by the fluorescent oxygen sensor in vacuum and a plurality of second phase shift values measured in a plurality of different known oxygen concentration environments; fitting a multi-site oxygen concentration calculation formula with unknown parameters according to the first phase shift value, the plurality of second phase shift values and the plurality of known oxygen concentrations to obtain values of the parameters in the multi-site oxygen concentration calculation formula; and obtaining a multi-site oxygen concentration calculation formula with known parameters according to the values of the parameters.
In an embodiment of the present invention, the fitting a multi-site oxygen concentration calculation formula with unknown parameters according to the first phase shift value, the plurality of second phase shift values, and the plurality of known oxygen concentrations to obtain the values of the parameters in the multi-site oxygen concentration calculation formula includes: circularly taking values of the parameters in a preset value range of the parameters to obtain values of a plurality of parameters; calculating to obtain a plurality of calculated oxygen concentration curves according to the values of the parameters, the first phase shift values, the second phase shift values and the known oxygen concentrations; obtaining a real oxygen concentration curve according to the plurality of second phase shift values and the plurality of known oxygen concentrations; setting a calculated oxygen concentration curve, which is closest to the true oxygen concentration curve, among the plurality of calculated oxygen concentration curves as a target curve; and taking the value of the parameter corresponding to the target curve as the value of the parameter in the multi-site oxygen concentration calculation formula.
In one embodiment of the present invention, the setting, as the target curve, a calculated oxygen concentration curve that is closest to a true oxygen concentration curve among the plurality of calculated oxygen concentration curves includes: obtaining a plurality of difference curves according to the plurality of calculated oxygen concentration curves and the real oxygen concentration curve; calculating a plurality of closeness degrees of the plurality of calculated oxygen concentration curves and the real oxygen concentration curve respectively according to the plurality of difference curves; the calculated oxygen concentration curve with the minimum proximity is taken as the target curve.
In an embodiment of the present invention, the calculating a plurality of closeness of the plurality of calculated oxygen concentration curves to the real oxygen concentration curve respectively according to the plurality of difference curves includes: and calculating the sum of the absolute values of the differences according to the difference curves to obtain the proximity curves.
In order to achieve the above object, a second embodiment of the present invention provides an oxygen concentration testing apparatus, including: the first acquisition module is used for acquiring a current phase shift value measured by the fluorescent oxygen sensor in a current oxygen concentration environment; the second acquisition module is used for acquiring a multi-site oxygen concentration calculation formula with known parameters; and the calculation module is used for calculating and obtaining the current oxygen concentration in the current oxygen concentration environment according to the current phase shift value and a multi-point oxygen concentration calculation formula with known parameters.
According to the oxygen concentration testing device provided by the embodiment of the invention, the current phase shift value measured by the fluorescent oxygen sensor in the current oxygen concentration environment is obtained through the first obtaining module, the multi-point oxygen concentration calculation formula with known parameters is obtained through the second obtaining module, and then the current oxygen concentration in the current oxygen concentration environment is calculated and obtained through the calculation module according to the current phase shift value and the multi-point oxygen concentration calculation formula with known parameters, so that the accuracy of the oxygen concentration test is greatly improved.
In addition, the oxygen concentration testing device proposed according to the above embodiment of the present invention may also have the following additional technical features:
in an embodiment of the present invention, the second obtaining module is specifically configured to: acquiring a first phase shift value measured by the fluorescent oxygen sensor in vacuum and a plurality of second phase shift values measured in a plurality of different known oxygen concentration environments; fitting a multi-site oxygen concentration calculation formula with unknown parameters according to the first phase shift value, the plurality of second phase shift values and the plurality of known oxygen concentrations to obtain values of the parameters in the multi-site oxygen concentration calculation formula; and obtaining a multi-site oxygen concentration calculation formula with known parameters according to the values of the parameters.
In an embodiment of the present invention, the calculation module is specifically configured to: circularly taking values of the parameters in a preset value range of the parameters to obtain values of a plurality of parameters; calculating to obtain a plurality of calculated oxygen concentration curves according to the values of the parameters, the first phase shift values, the second phase shift values and the known oxygen concentrations; obtaining a real oxygen concentration curve according to the plurality of second phase shift values and the plurality of known oxygen concentrations; setting a calculated oxygen concentration curve, which is closest to the true oxygen concentration curve, among the plurality of calculated oxygen concentration curves as a target curve; and taking the value of the parameter corresponding to the target curve as the value of the parameter in the multi-site oxygen concentration calculation formula.
In an embodiment of the present invention, the calculation module is specifically configured to: obtaining a plurality of difference curves according to the plurality of calculated oxygen concentration curves and the real oxygen concentration curve; calculating a plurality of closeness degrees of the plurality of calculated oxygen concentration curves and the real oxygen concentration curve respectively according to the plurality of difference curves; the calculated oxygen concentration curve with the minimum proximity is taken as the target curve.
In order to achieve the above object, a fluorescent oxygen sensor according to an embodiment of a third aspect of the present invention includes: the oxygen concentration test apparatus according to the embodiment of the second aspect of the present invention.
According to the fluorescent oxygen sensor provided by the embodiment of the invention, the accuracy of oxygen concentration test can be greatly improved through the oxygen concentration testing device.
Advantages of additional aspects of the invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention.
Drawings
The foregoing and/or additional aspects and advantages of the present invention will become apparent and readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings of which:
FIG. 1 is a flow chart of a method of testing oxygen concentration according to one embodiment of the present invention;
FIG. 2 is a logical schematic of the fluorescence quenching principle;
FIG. 3 is a flow chart of a method of testing oxygen concentration according to another embodiment of the present invention;
FIG. 4 is a diagram illustrating calibration parameters in a method for testing oxygen concentration according to an embodiment of the present invention;
FIG. 5 is a graph illustrating a difference curve according to an embodiment of the present invention;
FIG. 6 is a schematic illustration of a calculated oxygen concentration curve according to an embodiment of the present invention;
FIG. 7 is a schematic diagram of an optimal curve and an absolute curve according to an embodiment of the present invention; and
fig. 8 is a block schematic diagram of an oxygen concentration test apparatus according to an embodiment of the present invention.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are illustrative and intended to be illustrative of the invention and are not to be construed as limiting the invention.
An oxygen concentration test method, an oxygen concentration test apparatus, and a fluorescent oxygen sensor according to embodiments of the present invention will be described below with reference to the drawings.
FIG. 1 is a flow chart of a method of testing oxygen concentration according to one embodiment of the present invention. The oxygen concentration test method of the embodiment of the invention can be applied to an oxygen sensor, such as a fluorescent oxygen sensor (also known as a fluorescence quenching oxygen sensor). It should be noted that the oxygen sensor described in this embodiment may be installed in an exhaust pipe of an automobile to detect the concentration of oxygen in exhaust gas.
The general method for detecting the current oxygen concentration in the current oxygen concentration environment by the fluorescent oxygen sensor is to obtain a quenching constant by using a Stern-Volmer equation through straight line fitting, namely obtaining an oxygen concentration calculation formula of the sensor, and calculating the oxygen concentration in the current oxygen concentration environment by using the oxygen concentration calculation formula.
It should be noted that, according to the principle of fluorescence quenching of the fluorescent oxygen sensor in this embodiment, as shown in fig. 2, a fluorescent molecule in the ground state absorbs light energy having the same characteristic frequency as that of the fluorescent molecule, and transitions to each energy level of the excited state. Since molecules excited to respective vibrational levels of the excited state are unstable, they eventually fall to respective vibrational levels of the ground state and scatter out a response light quantum, which is called fluorescence. And each vibration energy level of the final ground state molecule returns to the lowest vibration energy level of the ground state through radiationless transition. Since quenching of fluorescence by a fluorescent substance and oxygen simultaneously leads to a decrease in fluorescence lifetime and a decrease in fluorescence intensity, the concentration or content of oxygen can be evaluated by measuring the decrease in fluorescence lifetime or the decrease in fluorescence intensity.
For fluorescent oxygen sensors, oxygen concentration metersThe quenching constant K is obtained by straight line fitting by generally using a Stern-Volmer equation, and the oxygen concentration calculation formula (1) of the sensor can be obtained:
wherein, I0、I、τ0τ is the fluorescence intensity and lifetime in the absence and presence of oxygen, x (O)2) K is the Stern-Volmer quenching constant for oxygen concentration, and is fixed for a particular quencher. For the measurement of fluorescence intensity and lifetime, it can be calculated by phase shift,
wherein the fluorescence lifetime is determined by phase shift, the excitation light is a sinusoidally modulated light signal, and the fluorescence signal emitted by the fluorescent substance also varies sinusoidally, and a is delayed by a certain value from the excitation light due to the time delay between light absorption and emission0And a are phase shifts in the absence and presence of oxygen, respectively. The oxygen concentration can thus be calculated by testing the phase shift a.
However, for many fluorescent oxygen sensors, the quenching phenomenon is accomplished in a heterogeneous medium, so the Stern-Volmer line equation based on homogeneous solution physicochemical considerations is not necessarily appropriate, as is the case with solid-supported membrane sensors. For equations that deviate from the Stern-Volmer equation, as in the multi-site equation (2):
wherein, Ksv1And Ksv2The quenching constants of the quenching processes of the freely soluble and aggregated molecules, respectively, f1And f2The relative distribution coefficients of free dissolved and aggregated molecules, respectively.
In order to solve the above-mentioned problem of the prior art regarding the fluorescent oxygen sensor to test the oxygen concentration, an embodiment of the present invention provides an oxygen concentration testing method, as shown in fig. 1, the oxygen concentration testing method of the embodiment of the present invention may include the following steps:
s1, acquiring a current phase shift value measured by the fluorescence oxygen sensor in the current oxygen concentration environment;
s2, acquiring a multi-site oxygen concentration calculation formula with known parameters;
and S3, calculating to obtain the current oxygen concentration in the current oxygen concentration environment according to the current phase shift value and a multi-point oxygen concentration calculation formula with known parameters.
For example, it is assumed that the fluorescent oxygen sensor to which the oxygen concentration test method of the embodiment of the present invention is applied is installed in an exhaust pipe of an automobile, wherein when the automobile is started, a current phase shift value measured in real time by the fluorescent oxygen sensor installed in the exhaust pipe is obtained, and a current oxygen concentration in an environment of the current oxygen concentration, that is, an oxygen concentration in exhaust gas of the automobile is calculated according to the current phase shift value and a multi-point oxygen concentration calculation formula with known parameters.
How to obtain the multi-site oxygen concentration calculation formula with known parameters is described in detail below:
in an embodiment of the present invention, as shown in fig. 3, the above oxygen concentration test method, S2, may include the following steps:
s21, acquiring a first phase shift value measured by the fluorescent oxygen sensor in vacuum and a plurality of second phase shift values measured in a plurality of different known oxygen concentration environments;
and S22, fitting the multi-site oxygen concentration calculation formula with unknown parameters according to the first phase shift value, the plurality of second phase shift values and the plurality of known oxygen concentrations to obtain the values of the parameters in the multi-site oxygen concentration calculation formula.
Wherein, the step S22 may include: the method comprises the steps of circularly taking values of parameters in a preset value range of the parameters to obtain values of a plurality of parameters, calculating to obtain a plurality of calculated oxygen concentration curves according to the values of the parameters, a first phase shift value, a plurality of second phase shift values and a plurality of known oxygen concentrations, obtaining a real oxygen concentration curve according to the second phase shift values and the known oxygen concentrations, taking a calculated oxygen concentration curve which is closest to the real oxygen concentration curve in the calculated oxygen concentration curves as a target curve, and taking a value of a parameter corresponding to the target curve as a value of the parameter in a multi-site oxygen concentration calculation formula. The preset value range can be calibrated according to actual conditions.
Further, taking as the target curve the calculated oxygen concentration curve that is closest to the true oxygen concentration curve among the plurality of calculated oxygen concentration curves may include: and obtaining a plurality of difference curves according to the plurality of calculated oxygen concentration curves and the real oxygen concentration curve, obtaining a plurality of closeness degrees of the plurality of calculated oxygen concentration curves and the real oxygen concentration curve according to the plurality of difference curves, and taking the calculated oxygen concentration curve with the minimum closeness degree as a target curve. Wherein, further, calculating a plurality of closeness of the plurality of calculated oxygen concentration curves with the real oxygen concentration curve according to the plurality of difference curves may include: and calculating the sum of the absolute values of the differences according to the difference curves to obtain a plurality of nearness values.
And S23, obtaining a multi-site oxygen concentration calculation formula with known parameters according to the values of the parameters.
Therefore, the current oxygen concentration in the current oxygen concentration environment is calculated through the acquired current phase shift value in the current oxygen concentration environment and a multi-site oxygen concentration calculation formula with known parameters, and the accuracy of the oxygen concentration test can be greatly improved.
The oxygen concentration test method according to the embodiment of the present invention will be described in detail below with reference to FIGS. 4 to 7:
assume that the fluorescence indicator in the fluorescent oxygen sensor to which the oxygen concentration test method of the embodiment of the present invention is applied is platinum octaethylporphyrin (PtOEP).
First, the corresponding phase shifts were tested under different oxygen concentrations, where the phase shift value measured in vacuum was a0The measured phase shift in the aerobic environment is a (i.e., the current phase shift value measured by the fluorescent oxygen sensor in the current oxygen concentration environment). Assuming the quenching coefficient K of freely soluble molecules in a multi-site modelsv1Quenching coefficient K for aggregating moleculessv2M times of, then Ksv1＝mKsv2. The total molecular distribution coefficient is 1, then f2＝1-f1。
The multi-site model may then be:
A*[O]2+B*[O]+C＝0，
the oxygen concentration was calculated as:
wherein the content of the first and second substances,
the oxygen concentration of the calibrated test environment is known, namely three parameters K in the oxygen concentration calculation formula are fitted according to actual test datasv1，f1And m, the oxygen concentration [ O ] can be obtained]And calculating the relation with the measured phase shift a value.
The idea of the calibration parameter calculation method can be shown in fig. 4. Three parameters K in equation (3)sv1，f1And m, calculating the oxygen concentration of different phase shifts under all combinations of the three parameters for different values of the three parameters to obtain a calculated oxygen concentration curve, comparing the calculated oxygen concentration curve with the real oxygen concentration curve of the environment, and when the calculated oxygen concentration curve is closest to the real oxygen concentration curve, calculating three parameters K corresponding to the calculated oxygen concentration curve (namely, a target curve)sv1，f1And the value of m can be the best parameter value for fitting and calibration.
The following describes the calculation process in detail:
quenching coefficient of general free-soluble moleculessv1Quenching coefficient K for aggregating moleculessv2And m can be 25-35 times of m, and the value interval of the cyclic calculation can be 0.01.
f1 is the relative distribution coefficient of free soluble molecules, the value is between 0 and 1, and the value interval of the cycle calculation can be 0.001.
Quenching constant K according to Stern-Volmer linear equationsvThe value interval of the cycle calculation is about 0.04-0.07, and the value interval of the cycle calculation can be 0.001.
Phase shift a at oxygen concentrationBetween 0 and 100 percent, and one for each 5 percent to obtain a0～a21Wherein a is0I.e. the phase shift in the absence of oxygen.
The real oxygen concentration curve was subtracted from the calculated oxygen concentration curve for each time to obtain a difference curve, as shown in fig. 5.
The absolute values of each difference are taken and summed to obtain the closeness s of the curve. Find the minimum s in the calculation of the cycle, i.e., the calculated oxygen concentration curve closest to the absolute curve (i.e., the target curve), which corresponds to Ksv1,f1And the m combination value is the optimal value of the three fitting parameters. As shown in FIG. 6, s is the smallest, at which time K issv1＝0.055，f10.807, and 29.36. And substituting the three parameters into the formula (3) to obtain a calibrated calculation formula. Wherein, the calculated optimal curve (i.e. the target curve) and the absolute curve can be shown in fig. seven.
In summary, according to the oxygen concentration testing method of the embodiment of the invention, the current phase shift value measured by the fluorescent oxygen sensor in the current oxygen concentration environment is obtained, and then the current oxygen concentration in the current oxygen concentration environment is calculated according to the current phase shift value and the multi-point oxygen concentration calculation formula with known parameters, so that the accuracy of the oxygen concentration test is greatly improved.
Fig. 8 is a block schematic diagram of an oxygen concentration test apparatus according to an embodiment of the present invention.
As shown in fig. 8, the oxygen concentration test apparatus according to the embodiment of the present invention may include: a first acquisition module 100, a second acquisition module 200, and a calculation module 300.
The first obtaining module 100 is configured to obtain a current phase shift value measured by the fluorescent oxygen sensor in a current oxygen concentration environment.
A second obtaining module 200 for obtaining a multi-site oxygen concentration calculation formula with known parameters.
And a calculating module 300, configured to calculate a current oxygen concentration in the current oxygen concentration environment according to the current phase shift value and a multi-point oxygen concentration calculation formula with known parameters.
In an embodiment of the invention, the second obtaining module 200 is specifically configured to obtain a first phase shift value measured by the fluorescent oxygen sensor in vacuum and a plurality of second phase shift values measured in a plurality of different known oxygen concentration environments, and fit a multi-site oxygen concentration calculation formula with unknown parameters according to the first phase shift value, the plurality of second phase shift values, and the plurality of known oxygen concentrations to obtain values of parameters in the multi-site oxygen concentration calculation formula, and obtain the multi-site oxygen concentration calculation formula with known parameters according to the values of the parameters.
In an embodiment of the present invention, the computing module 200 is specifically configured to: circularly taking values of the parameters in a preset value range of the parameters to obtain values of a plurality of parameters; calculating to obtain a plurality of calculated oxygen concentration curves according to the values of the parameters, the first phase shift value, the second phase shift values and the known oxygen concentrations; obtaining a real oxygen concentration curve according to the plurality of second phase shift values and the plurality of known oxygen concentrations; and taking the calculated oxygen concentration curve which is closest to the real oxygen concentration curve in the plurality of calculated oxygen concentration curves as a target curve, and taking the value of the parameter corresponding to the target curve as the value of the parameter in the multi-point oxygen concentration calculation formula.
In an embodiment of the present invention, the computing module 200 is further specifically configured to: and obtaining a plurality of difference curves according to the plurality of calculated oxygen concentration curves and the real oxygen concentration curve, obtaining a plurality of proximity degrees of the plurality of calculated oxygen concentration curves and the real oxygen concentration curve respectively according to the plurality of difference curves, and taking the calculated oxygen concentration curve with the minimum proximity degree as a target curve.
It should be noted that, for details not disclosed in the oxygen concentration testing method of the embodiment of the present invention, please refer to details disclosed in the oxygen concentration testing apparatus of the embodiment of the present invention, which are not repeated herein.
To sum up, the oxygen concentration testing device according to the embodiment of the present invention first obtains the current phase shift value measured by the fluorescent oxygen sensor in the current oxygen concentration environment through the obtaining module, and then obtains the current oxygen concentration in the current oxygen concentration environment through calculation according to the current phase shift value and the multi-point oxygen concentration calculation formula with known parameters through the calculation module, thereby greatly improving the accuracy of the oxygen concentration test.
In order to implement the above embodiments, the present invention further provides a fluorescent oxygen sensor, which includes the above oxygen concentration testing apparatus.
According to the fluorescent oxygen sensor provided by the embodiment of the invention, the accuracy of oxygen concentration test can be greatly improved through the oxygen concentration testing device.
In the description of the present invention, it is to be understood that the terms "central," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," "circumferential," and the like are used in the orientations and positional relationships indicated in the drawings for convenience in describing the invention and to simplify the description, and are not intended to indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and are not to be considered limiting of the invention.
Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.
In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can, for example, be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
In the present invention, unless otherwise expressly stated or limited, the first feature "on" or "under" the second feature may be directly contacting the first and second features or indirectly contacting the first and second features through an intermediate. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.
In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above are not necessarily intended to refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples and features of different embodiments or examples described in this specification can be combined and combined by one skilled in the art without contradiction.
Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art within the scope of the present invention.
Claims (10)
1. An oxygen concentration test method is characterized by comprising the following steps:
acquiring a current phase shift value measured by a fluorescent oxygen sensor in a current oxygen concentration environment;
acquiring a multi-site oxygen concentration calculation formula with known parameters;
and calculating to obtain the current oxygen concentration in the current oxygen concentration environment according to the current phase shift value and a multi-site oxygen concentration calculation formula with known parameters.
2. The oxygen concentration test method according to claim 1, wherein the multi-site oxygen concentration calculation formula in which the acquisition parameter is known includes:
acquiring a first phase shift value measured by the fluorescent oxygen sensor in vacuum and a plurality of second phase shift values measured in a plurality of different known oxygen concentration environments;
fitting a multi-site oxygen concentration calculation formula with unknown parameters according to the first phase shift value, the plurality of second phase shift values and the plurality of known oxygen concentrations to obtain values of the parameters in the multi-site oxygen concentration calculation formula;
and obtaining a multi-site oxygen concentration calculation formula with known parameters according to the values of the parameters.
3. The method according to claim 2, wherein the fitting a multi-site oxygen concentration calculation formula with unknown parameters according to the first phase shift value, the plurality of second phase shift values, and the plurality of known oxygen concentrations to obtain the values of the parameters in the multi-site oxygen concentration calculation formula comprises:
circularly taking values of the parameters in a preset value range of the parameters to obtain values of a plurality of parameters;
calculating to obtain a plurality of calculated oxygen concentration curves according to the values of the parameters, the first phase shift values, the second phase shift values and the known oxygen concentrations;
obtaining a real oxygen concentration curve according to the plurality of second phase shift values and the plurality of known oxygen concentrations;
setting a calculated oxygen concentration curve, which is closest to the true oxygen concentration curve, among the plurality of calculated oxygen concentration curves as a target curve;
and taking the value of the parameter corresponding to the target curve as the value of the parameter in the multi-site oxygen concentration calculation formula.
4. The oxygen concentration test method according to claim 3, wherein the setting, as a target curve, a calculated oxygen concentration curve that is closest to a true oxygen concentration curve among the plurality of calculated oxygen concentration curves includes:
obtaining a plurality of difference curves according to the plurality of calculated oxygen concentration curves and the real oxygen concentration curve;
calculating a plurality of closeness degrees of the plurality of calculated oxygen concentration curves and the real oxygen concentration curve respectively according to the plurality of difference curves;
the calculated oxygen concentration curve with the minimum proximity is taken as the target curve.
5. The oxygen concentration test method according to claim 4, wherein the calculating a plurality of closeness of the plurality of calculated oxygen concentration curves to the true oxygen concentration curve, respectively, from the plurality of difference curves comprises:
and calculating the sum of the absolute values of the differences according to the difference curves to obtain the proximity curves.
6. An oxygen concentration test apparatus, characterized by comprising:
the first acquisition module is used for acquiring a current phase shift value measured by the fluorescent oxygen sensor in a current oxygen concentration environment;
the second acquisition module is used for acquiring a multi-site oxygen concentration calculation formula with known parameters;
and the calculation module is used for calculating and obtaining the current oxygen concentration in the current oxygen concentration environment according to the current phase shift value and a multi-point oxygen concentration calculation formula with known parameters.
7. The oxygen concentration test apparatus according to claim 6, wherein the second obtaining module is specifically configured to:
acquiring a first phase shift value measured by the fluorescent oxygen sensor in vacuum and a plurality of second phase shift values measured in a plurality of different known oxygen concentration environments;
fitting a multi-site oxygen concentration calculation formula with unknown parameters according to the first phase shift value, the plurality of second phase shift values and the plurality of known oxygen concentrations to obtain values of the parameters in the multi-site oxygen concentration calculation formula;
and obtaining a multi-site oxygen concentration calculation formula with known parameters according to the values of the parameters.
8. The oxygen concentration test apparatus according to claim 7, wherein the calculation module is specifically configured to:
circularly taking values of the parameters in a preset value range of the parameters to obtain values of a plurality of parameters;
calculating to obtain a plurality of calculated oxygen concentration curves according to the values of the parameters, the first phase shift values, the second phase shift values and the known oxygen concentrations;
obtaining a real oxygen concentration curve according to the plurality of second phase shift values and the plurality of known oxygen concentrations;
setting a calculated oxygen concentration curve, which is closest to the true oxygen concentration curve, among the plurality of calculated oxygen concentration curves as a target curve;
and taking the value of the parameter corresponding to the target curve as the value of the parameter in the multi-site oxygen concentration calculation formula.
9. The oxygen concentration test apparatus according to claim 8, wherein the calculation module is specifically configured to:
obtaining a plurality of difference curves according to the plurality of calculated oxygen concentration curves and the real oxygen concentration curve;
calculating a plurality of closeness degrees of the plurality of calculated oxygen concentration curves and the real oxygen concentration curve respectively according to the plurality of difference curves;
the calculated oxygen concentration curve with the minimum proximity is taken as the target curve.
10. A fluorescent oxygen sensor, comprising: the oxygen concentration test apparatus according to any one of claims 6 to 9.
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