CN113025680A - Method for fermentation conversion of madecassoside and application thereof - Google Patents
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Abstract
The invention relates to a centella asiatica fermentation liquid for converting high-content madecassoside and producing high-activity asiatic acid and derivatives thereof by utilizing a microbial conversion reaction, a preparation method and an application thereof, wherein centella asiatica is fermented by thermophilic thermus bacteria to convert madecassoside and asiatic acid, wherein the madecassoside content is increased by 200-8000 times, and the efficacy of the fermented centella asiatica is as follows: anti-inflammatory, wound repair, oxidation resistance, whitening and skin cell repair capabilities (photodamage repair and scratch repair) are also remarkably improved.
Description
Technical Field
The invention belongs to the field of fermentation, relates to centella asiatica fermentation, and particularly relates to a preparation method for converting a fermentation liquor rich in madecassoside by utilizing a microbial conversion reaction, and an application of the madecassoside fermentation liquor.
Background
Centella asiatica is centella asiatica (L.) Urban, also known as centella asiatica, pennisetum asiaticum, and corm Eleocharitis, which are the whole herbs of centella asiatica of Umbelliferae. Centella asiatica is native to india, is widely distributed in tropical and subtropical regions of the world, and is mainly distributed in south provinces of Yangtze river in China. The main active ingredients of centella asiatica include asiaticoside, madecassoside, asiatic acid and madecassic acid. Centella has long been used as a brain tonic, diuretic, and adjuvant for leprosy, ulcers, and dermatological drug.
The natural medicine centella has strong effects of promoting wound healing and skin repair, can inhibit proliferation of skin scar fibroblasts, reduce synthesis of extracellular matrix, stimulate fibroblast proliferation, well induce a cell withering blocker, reduce oxygen radical concentration, obviously resist bacteria, reduce inflammation, promote regeneration and repair of scar epidermis, lighten pigments (promote apoptosis of melanocytes and inhibit synthesis of melanin), promote cell regeneration and restore skin elasticity and luster.
Centella asiatica has complex chemical components, and mainly comprises triterpenes, flavonoids, volatile oils, polyacetylenes and the like. It is currently believed that the major active ingredient of centella asiatica is total asiaticoside, which is a mixture of glycosides formed mainly by triterpenic alkanoic acids, including asiaticoside-B, madecassoside and asiaticoside, all of which have pentacyclic triterpene structures and glycoside branches, differing only in the number and position of the hydroxyl groups on the triterpene rings. The effective effect of asiatic centella is asiaticoside and madecassoside content, and research shows that the effect of madecassoside is obviously stronger than that of asiaticoside.
CN101724006A discloses a method for separating asiaticoside-B, madecassoside and asiaticoside, comprising: dissolving the raw material in methanol aqueous solution, introducing into a simulated moving bed chromatographic system, eluting with methanol aqueous solution to obtain asiaticoside, and evaporating raffinate to remove solvent to obtain primary solid material; dissolving the primary solid material in an aqueous solution containing acetonitrile and methyl tert-butyl ether or introducing the aqueous solution into a simulated moving bed chromatographic system, eluting the aqueous solution containing acetonitrile and methyl tert-butyl ether to obtain madecassoside, and evaporating raffinate to remove the solvent to obtain a secondary solid material; dissolving the secondary solid material in methanol water solution, introducing into a simulated moving bed chromatographic system, and eluting with methanol water solution to obtain asiaticoside-B. However, the method starts from raw materials containing the asiaticoside, adopts a simulated moving bed chromatography system, has complex operation and high equipment requirement, uses methyl tert-butyl ether, has certain toxicity and danger, and needs to pay full attention to the storage and use processes.
CN102477063 discloses a method for separating asiaticoside B and madecassoside in an asiatic pennywort herb extract by a preparative high performance liquid chromatograph, cyclodextrin is added into a mobile phase, and then organic solvent is used for extracting and removing the cyclodextrin in a solvent to obtain a pure product. CN102532244A discloses a method for preparing high-purity asiaticoside, which is obtained by extracting, decoloring and recrystallizing, but various organic solvents are adopted in the process to extract the extract of centella asiatica, so that the dosage of the organic solvents is large, and secondary pollution is easily caused. CN101948501A discloses a method for preparing madecassoside, the extract is passed through macroporous resin column layer, active carbon decolorization, crystallization, macroporous resin re-chromatography, and finally the madecassoside with purity of more than 98% is obtained.
The methods for preparing the madecassoside by extraction have the advantages of long period, complex process and high cost.
Due to the complexity and the particularity of the properties of each component in centella asiatica, particularly the structural and physicochemical properties of asiaticoside, madecassoside and asiaticoside B in the total asiaticoside are all relatively close, wherein the madecassoside and the asiaticoside B are a pair of isomers, compared with the asiaticoside, the madecassoside only has one more hydroxyl group, and the connection of the carbohydrate with strong polarity greatly weakens the difference of tiny polarity, so that the three substances are difficult to be completely separated by using a conventional separation method. The prior art either mostly prepares the three compounds from asiaticoside or prepares and separates one or two compounds from asiatic centella. However, the use of microorganisms for fermenting centella asiatica to obtain madecassoside through biotransformation has not been reported at present.
Disclosure of Invention
The invention provides a preparation method of madecassoside fermentation conversion, which comprises the steps of producing a centella asiatica fermentation liquid rich in madecassoside by fermenting a microorganism containing thermophilic bacteria, converting high-content madecassoside, or obtaining the centella asiatica fermentation liquid rich in madecassoside by fermenting a centella asiatica extract or asiaticoside by using the microorganism containing thermophilic bacteria, wherein the polarity of the asiaticoside is improved after the asiaticoside is subjected to hydroxyl conversion, so that the utilization rate of the asiaticoside component is improved. Meanwhile, compared with methods such as direct extraction of medicinal materials, the yield of the madecassoside prepared by the process is higher after conversion, is 8000 times of that of a water extracting solution with the same concentration, and the content of the madecassoside is greatly increased.
On the other hand, the invention aims to provide the asiatic centella fermentation liquid rich in madecassoside, which is used for fermenting the asiatic centella through thermophilic bacteria or a multi-bacteria composition consisting of thermophilic bacteria and other bacterial strains, so that the utilization rate of the asiatic centella is improved, and the clinical efficacy is improved.
The third aspect of the invention is to provide the application of the asiaticoside-rich centella asiatica fermentation broth in the field of cosmetics, wherein the oxidation resistance, whitening and skin cell repair capabilities (photodamage repair and scratch repair) are remarkably improved (compared with the pure thermus thermophilus fermentation broth).
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for fermenting and converting madecassoside, which comprises the following steps: taking centella asiatica or a crude extract of centella asiatica or asiaticoside or a composition of more than two as raw materials, and preparing fermentation liquor rich in madecassoside by microbial fermentation;
or preparing fermentation liquor rich in madecassoside by taking asiatic pennywort herb fermentation liquor or asiatic pennywort herb crude extract or asiaticoside or a composition of more than two of the asiaticoside and the asiaticoside as raw materials through microbial secondary fermentation;
post-treating the fermentation liquor rich in madecassoside to obtain a madecassoside pure product;
wherein the microorganism comprises thermophilic bacteria or a multi-bacteria composition consisting of thermophilic bacteria and non-thermophilic bacteria.
In the invention, after being fermented by thermophilic microorganisms, asiatic centella or asiatic centella crude extracts or asiaticoside or the composition thereof are converted to generate madecassoside, wherein the content of the madecassoside is 200-8000 times of that of asiatic centella aqueous extract with the same concentration.
As a preferable mode of the present invention, the thermophilic bacteria includes one of thermophilic Thermus, Streptococcus thermophilus, Bacillus thermophilus and Thermotoga maritima.
As a preferred embodiment of the present invention, the non-thermophilic bacteria comprise one or more of lactic acid bacteria, bifidobacteria, aspergillus oryzae or yeast.
In a preferred embodiment of the present invention, the thermophilic bacterium is a thermophilic thermus thermophilus.
In a preferred embodiment of the present invention, the addition amount of centella asiatica is 0.001-2000g/L, and the addition amount of the crude extract of centella asiatica is 0.001-2000 g/L; the addition amount of the centella asiatica fermentation liquid is 0.1-99.9%.
As a preferable scheme of the invention, when the microorganism is thermophilic bacteria, the fermentation temperature is 60-80 ℃, and the fermentation time is 2-80 h.
As a preferable scheme of the invention, when the microorganism is a multi-bacterium composition, the fermentation temperature is 25-80 ℃, and the fermentation time is 2-80 h.
In a preferable embodiment of the present invention, the thermophilic bacteria accounts for 2% -99.9% of the multi-bacteria composition.
Application of asiaticoside-rich centella fermentation broth in the field of cosmetics is provided.
Preferably, the yield of the madecassoside obtained by microbial transformation is more than 200 times of the yield of the direct extraction of the medicinal materials.
The invention has the following beneficial effects:
1) centella asiatica is fermented by thermophilic bacteria to generate madecassoside, and the madecassoside content is 8000 times of that of the water extract of centella asiatica with the same concentration, namely 200-.
2) After centella asiatica is fermented, the total polysaccharide content is increased by 19 times, the amino acid content is increased by 12 times, the total polysaccharide content is increased by 108 times, the total flavone content is increased by 145 times, the water-soluble solid content is increased by 8 times, the total polysaccharide content is increased by 6 times, the total flavone content is increased by 11 times, the total polyphenol content is increased by 6 times, the total amino acid content is increased by 42 times, and the water-soluble solid content is increased by 10 times.
3) The efficacy of fermented centella asiatica [ anti-oxidation, whitening and skin cell repair capabilities (photodamage repair and scratch repair) ] is also remarkably improved.
Drawings
FIG. 1 is a graph showing the growth rate of centella asiatica fermentation broth compared with that of pure Thermus thermophilus fermentation broth.
FIG. 2 is a schematic diagram showing the increase rate of centella asiatica fermentation liquid in comparison with the concentrated solution of centella asiatica water extract.
FIG. 3 is an HPLC chart of madecassic acid and an asiatic acid standard.
FIG. 4 is an HPLC chart of an aqueous extract concentrate of centella asiatica in an asiatic acid conversion product.
FIG. 5 is an HPLC chart of a fermentation broth of centella asiatica in a centella asiatica conversion product.
FIG. 6 is an HPLC chart of madecassoside and asiaticoside standard.
FIG. 7 is an HPLC chart of an aqueous extract concentrate of centella asiatica from a centella asiatica glycosides conversion product.
FIG. 8 is an HPLC chart of a fermentation broth of centella asiatica in a centella asiatica glycosides transformant.
FIG. 9 is a graph comparing antioxidant capacity (scavenging DPPH).
FIG. 10 is a schematic representation of tyrosinase activity in B16 cells.
FIG. 11 is a graph showing the photodamage repair ability of centella asiatica-Thermus thermophilus fermentation broth on HaCAT cells at various concentrations.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The reagents used in the present invention are commercially available, and the equipment used is also commercially available.
The invention provides a fermentation conversion method of madecassoside, which comprises the following steps: using centella asiatica or a crude extract of centella asiatica or asiaticoside or a composition of more than two of the centella asiatica and the asiaticoside as raw materials, and preparing centella asiatica fermentation liquor rich in the madecassoside by microbial fermentation;
or, the asiatic centella fermentation liquor or the asiatic centella crude extract or the asiaticoside is used as a raw material to prepare the asiatic centella fermentation liquor rich in the madecassoside through microbial secondary fermentation;
post-treating the fermentation liquor rich in madecassoside to obtain a madecassoside pure product;
wherein the microorganism comprises thermophilic bacteria or a multi-bacteria composition consisting of thermophilic bacteria and non-thermophilic bacteria.
Preferably, the thermophilic bacteria comprises one of thermophilic thermus thermophilus, streptococcus thermophilus, bacillus thermophilus and thermatopax maritima.
Most preferably, the thermophilic bacterium is a thermophilic thermus thermophilus.
Preferably, the non-thermophilic bacteria comprise one or more of lactic acid bacteria, bifidobacteria, aspergillus oryzae or yeast.
The post-treatment comprises conventional processes such as separation and purification, crystallization and the like.
When the microorganism is thermophilic bacteria, the fermentation temperature is 60-80 ℃, and the fermentation time is 2-80 h.
When the microorganism is a multi-bacteria composition, the fermentation temperature is 25-80 ℃, and the fermentation time is 2-80 h.
The multi-bacterial composition can be a dual-bacterial composition of thermus thermophilus and lactic acid bacteria, a dual-bacterial composition of thermus thermophilus and bifidobacteria, a dual-bacterial composition of thermus thermophilus and aspergillus oryzae, a dual-bacterial composition of thermus thermophilus and saccharomycetes, a multi-bacterial composition of thermus thermophilus and lactic acid bacteria plus bifidobacteria, a multi-bacterial composition of thermus thermophilus and lactic acid bacteria plus aspergillus oryzae, a multi-bacterial composition of thermus thermophilus and lactic acid bacteria plus saccharomycetes, a multi-bacterial composition of thermus thermophilus and bifidobacterium plus aspergillus oryzae, a multi-bacterial composition of thermus thermophilus and lactic acid bacteria plus aspergillus oryzae, a multi-bacterial composition of thermus thermophilus and lactobacillus plus aspergillus oryzae, a multi-bacterial composition of thermus and lactic acid bacteria plus bifidobacterium plus aspergillus oryzae, the composition comprises thermophilic thermus strain, lactobacillus, bifidobacterium and saccharomycetes, and the composition comprises thermophilic thermus strain, lactobacillus, bifidobacterium, aspergillus oryzae and saccharomycetes.
In the multi-bacterial composition, the Thermus thermophilus may also be Streptococcus thermophilus, Bacillus thermophilus, Thermotoga maritima.
Example 1
Adding centella into a thermus thermophilus culture medium, culturing and fermenting according to a fermentation process (60 ℃, 48 hours) of the thermus thermophilus, respectively selecting two additive concentrations of 5g/L and 50g/L for the centella, and carrying out analysis and comparison on a concentrated solution (concentrated medicinal material: the ratio of the concentrated solution is 2: 1, namely 2g of the medicinal material is obtained to obtain 1g of the concentrated solution) of the water extract of the centella with the same concentration and a pure thermus thermophilus fermentation liquid without the added centella, wherein the results are shown in a table 1 and a figure 2: (I) comparison of various ingredients
TABLE 1 comparison of the composition of the fermentation broth of centella asiatica with the fermentation broth of pure Thermus thermophilus, the aqueous extract of centella asiatica of the same concentration
As can be seen from fig. 1, fig. 2 and table 1, after centella asiatica is fermented, the total polysaccharide content is increased by 19 times, the amino acid content is increased by 12 times, the total polysaccharide content is increased by 108 times, the total flavone content is increased by 145 times, the water-soluble solid content is increased by 8 times, the madecassoside content is increased by 1520 times, the madecassoside content is increased by 6 times, the total polysaccharide content is increased by 6 times, the total flavone content is increased by 11 times, the total polyphenol content is increased by 6 times, the total amino acid content is increased by 42 times, and the water-soluble solid content is increased by 10 times.
(II) extraction and transformation of asiaticoside
By contrast, centella asiatica produces 4 similar components, such as asiatic acid and madecassic acid, by fermentation. See high performance liquid chromatography (fig. 3, fig. 4 and fig. 5):
by using a centella asiatica fermentation liquid obtained by fermenting thermophilic thermus and an asiatic pennywort herb water extract (concentrated into a medicinal material ratio concentrated solution of 2: 1) under the environmental conditions of the same temperature, the same time and the like, and comparing and observing related characteristic peaks of asiatic acid and hydroxyl area oxalic acid before and after the fermentation of the centella asiatica in a standard high performance liquid chromatography characteristic spectrum analysis system.
By comparison it can be seen that:
(1) in the centella asiatica water extraction concentrated solution (2: 1), the related characteristic peaks of asiatic acid and madecassic acid are not generated.
(2) In centella asiatica fermentation broth (5g/L), asiatic acid was produced by conversion.
(3) In the centella asiatica fermentation broth (5g/L), new characteristic peaks also appeared at 6.5 ', 7.6 ' and 20.2 '.
From the above map analysis, centella asiatica is fermented and transformed by thermophilic thermus strain to obtain 4 components such as asiatic acid and other asiatic acid analogues which are not contained in the water extract of centella asiatica. That is, centella asiatica produces 4 similar components such as asiatic acid by fermentation.
The third step of fermenting the asiatic pennywort herb by using the thermophilic thermus strain also discovers that related invertase in the thermophilic thermus strain can convert and enrich asiaticoside components (particularly madecassoside) in the asiatic pennywort herb, and the content of the madecassoside in the obtained asiatic pennywort herb fermentation liquid is greatly increased (compared with the water extract of the asiatic pennywort herb with the same concentration, the content of the converted asiaticoside can be increased by 1520 times). Referring to the high performance liquid chromatogram (FIG. 6, FIG. 7 and FIG. 8), the comparison shows that the centella asiatica is transformed by the fermentation of Thermus thermophilus to produce a large amount of madecassoside.
By using a centella asiatica fermentation liquid fermented by thermophilic thermus bacteria and a centella asiatica water extract (concentrated into a concentrated solution with a ratio of 2: 1) under the environmental conditions of the same temperature, the same time and the like, the characteristic peaks related to asiaticoside and madecassoside before and after centella asiatica fermentation are contrastingly observed in a standard high performance liquid chromatography characteristic spectrum analysis system.
By comparison it can be seen that:
(1) in the concentrated solution of the asiaticoside extracted by the asiaticoside water, the related characteristic peaks of the asiaticoside and the madecassoside are avoided.
(2) In the graph of centella asiatica fermentation broth (5g/L), a large amount of madecassoside is produced by transformation. Through content calculation, the obtained madecassoside content in the centella asiatica fermentation liquid reaches up to 1.5 g/L. After fermentation and conversion, the content of madecassoside is improved by 1520 times compared with the medicinal material asiatic centella.
Therefore, the centella asiatica is fermented by thermophilic thermus bacteria to generate a large amount of madecassoside, and after fermentation and conversion, the conversion yield of the madecassoside is up to 20% of the total mass of the medicinal materials.
Example 2
Adding asiaticoside into culture medium of a double-bacterium composition of thermophilic thermus strain and lactobacillus (the addition amount is 50g/L), fermenting by lactobacillus at 28 deg.C for 18H, fermenting by thermophilic thermus strain at 78 deg.C for 8H, decolorizing the fermentation liquid in D330 resin at the flow rate of 2BV/H, centrifuging, filtering to obtain asiaticoside-enriched centella fermentation liquid, and performing conventional purification and crystallization to obtain pure madecassoside.
Example 3
Adding the crude extract (the adding amount is 10g/L) of the asiatic centella into a culture medium of streptococcus thermophilus, culturing and fermenting according to a fermentation process (65 ℃, 36H), adding the fermentation liquor into D330 resin, decoloring at the flow rate of 2BV/H, obtaining asiatic centella fermentation liquor rich in madecassoside after centrifugal filtration, and obtaining a pure madecassoside product through conventional post-treatment processes such as purification, crystallization and the like.
Example 4
The only difference is that the starting material is a centella asiatica fermentation broth, as in example 3.
Example 5
The comparison effect research of the centella asiatica fermentation liquid and the thermophilic thermus strain fermentation liquid comprises the following steps: see fig. 9, 10 and 11.
(1) Stronger oxidation resistance (anti-aging) capability;
(2) the whitening and spot-lightening efficacy is more excellent: the whitening activity of the centella asiatica fermentation liquid is stronger than that of the original fermentation liquid and a centella asiatica extract concentrated solution, and the tyrosinase inhibition rate of 5% of the centella asiatica (thermus thermophilus) fermentation liquid reaches 50%, so that the centella asiatica fermentation liquid shows stronger activity inhibition capability and has stronger whitening effect.
(3) And (3) repairing the photodamage: comparing a positive drug dexamethasone group with a group without ultraviolet irradiation, finding that the centella asiatica fermentation liquor has stronger repair capability on the optical damage of HaCAT cells after ultraviolet irradiation, the cell survival rate is more than 100% within the range of 3% -10%, the optical damage repair capability is stronger, and the effect of slightly promoting growth is achieved; the fermentation liquor of the asiatic pennywort herb/thermophilic thermus strain is proved to have stronger capability of repairing the photodamage of skin cells.
Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes in the embodiments and/or modifications of the invention can be made, and equivalents and modifications of some features of the invention can be made without departing from the spirit and scope of the invention.
Claims (10)
1. A method for fermenting and converting madecassoside, which is characterized in that the preparation method comprises the following steps: taking centella asiatica or a crude extract of centella asiatica or asiaticoside or a composition of more than two as raw materials, and preparing fermentation liquor rich in madecassoside by microbial fermentation;
or preparing fermentation liquor rich in madecassoside by taking asiatic pennywort herb fermentation liquor or asiatic pennywort herb crude extract or asiaticoside or a composition of more than two of the asiaticoside and the asiaticoside as raw materials through microbial secondary fermentation;
post-treating the fermentation liquor rich in madecassoside to obtain a madecassoside pure product;
wherein the microorganism comprises thermophilic bacteria or a multi-bacteria composition consisting of thermophilic bacteria and non-thermophilic bacteria.
2. The method of claim 1, wherein the thermophilic bacterium comprises one of thermophilic Thermus species, Streptococcus thermophilus, Bacillus thermophilus, or Thermotoga maritima.
3. The method of claim 1, wherein the non-thermophilic bacteria comprise one or more of lactic acid bacteria, bifidobacteria, aspergillus oryzae, or yeast.
4. A method for the fermentative conversion of madecassoside according to claim 1, 2 or 3, wherein the thermophilic bacterium is Thermus thermophilus.
5. The method for the fermentative conversion of madecassoside according to claim 1, characterized in that the centella asiatica is added in an amount of 0.001-2000 g/L.
6. The method for the fermentative conversion of madecassoside according to claim 1, characterized in that the crude extract of centella asiatica is added in an amount of 0.001-2000 g/L.
7. The method of claim 1, wherein the fermentation broth of centella asiatica is added in an amount of 0.01-99.9%.
8. The method for fermentation and conversion of madecassoside according to claim 1, wherein the thermophilic bacteria is present in the composition of 2-99.9%.
9. Use of a fermentation broth of centella asiatica rich in madecassoside, characterized in that madecassoside obtained by the fermentative conversion process according to any one of claims 1 to 8 or a fermentation broth rich in madecassoside is used in the cosmetic field.
10. The use of the fermentation broth of centella asiatica rich in madecassoside according to claim 9, wherein the yield of madecassoside obtained by the microbial transformation of claim 1 is more than 200 times that obtained by direct extraction of the same concentration of herbs.
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| CN113440465A (en) * | 2021-08-23 | 2021-09-28 | 云南英格生物技术有限公司 | Rice fermentation product, rice fermentation extract, preparation method and application |
| CN113509415A (en) * | 2021-07-08 | 2021-10-19 | 江苏瑞霆生物科技有限公司 | Preparation method of centella asiatica fermentation filtrate, product and application thereof |
| CN114557906A (en) * | 2022-03-11 | 2022-05-31 | 杭州友丽医疗科技(集团)有限公司 | Moisturizing and anti-aging collagen water-based polishing solution and preparation method thereof |
| CN114588070A (en) * | 2022-03-03 | 2022-06-07 | 深圳亿汀生物有限公司 | Composition for dynamically adjusting water-oil balance of skin to achieve rapid relief and application thereof |
| CN114763565A (en) * | 2022-05-09 | 2022-07-19 | 杭州优玛达生物科技有限公司 | Biotransformation method for improving water solubility of asiatic acid and fermentation mixed product prepared by same |
| CN115154403A (en) * | 2022-08-25 | 2022-10-11 | 广州阿基米德生物科技有限公司 | Skin repair composition and preparation method and application thereof |
| CN115737709A (en) * | 2022-09-08 | 2023-03-07 | 高文红 | Biological fermentation product and preparation method and application thereof |
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| CN113509415A (en) * | 2021-07-08 | 2021-10-19 | 江苏瑞霆生物科技有限公司 | Preparation method of centella asiatica fermentation filtrate, product and application thereof |
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| CN113440465A (en) * | 2021-08-23 | 2021-09-28 | 云南英格生物技术有限公司 | Rice fermentation product, rice fermentation extract, preparation method and application |
| CN114588070A (en) * | 2022-03-03 | 2022-06-07 | 深圳亿汀生物有限公司 | Composition for dynamically adjusting water-oil balance of skin to achieve rapid relief and application thereof |
| CN114557906A (en) * | 2022-03-11 | 2022-05-31 | 杭州友丽医疗科技(集团)有限公司 | Moisturizing and anti-aging collagen water-based polishing solution and preparation method thereof |
| CN114557906B (en) * | 2022-03-11 | 2024-02-20 | 杭州友丽医疗科技(集团)有限公司 | Moisturizing anti-aging collagen water gloss liquid and preparation method thereof |
| CN114763565A (en) * | 2022-05-09 | 2022-07-19 | 杭州优玛达生物科技有限公司 | Biotransformation method for improving water solubility of asiatic acid and fermentation mixed product prepared by same |
| CN114763565B (en) * | 2022-05-09 | 2023-08-22 | 杭州优玛达生物科技有限公司 | Bioconversion method for improving asiatic acid water solubility and fermentation mixed product prepared by same |
| CN115154403A (en) * | 2022-08-25 | 2022-10-11 | 广州阿基米德生物科技有限公司 | Skin repair composition and preparation method and application thereof |
| CN115154403B (en) * | 2022-08-25 | 2023-04-18 | 广州阿基米德生物科技有限公司 | Skin repair composition and preparation method and application thereof |
| CN115737709A (en) * | 2022-09-08 | 2023-03-07 | 高文红 | Biological fermentation product and preparation method and application thereof |
| CN115737709B (en) * | 2022-09-08 | 2024-01-26 | 高文红 | Biological fermentation product and preparation method and application thereof |
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