CN113009129A - Preparation method of colloidal gold immunochromatographic test strip - Google Patents
Preparation method of colloidal gold immunochromatographic test strip Download PDFInfo
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- CN113009129A CN113009129A CN202110331518.3A CN202110331518A CN113009129A CN 113009129 A CN113009129 A CN 113009129A CN 202110331518 A CN202110331518 A CN 202110331518A CN 113009129 A CN113009129 A CN 113009129A
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- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 title claims abstract description 106
- 238000002360 preparation method Methods 0.000 title claims abstract description 12
- 239000012528 membrane Substances 0.000 claims abstract description 43
- 239000001913 cellulose Substances 0.000 claims abstract description 33
- 229920002678 cellulose Polymers 0.000 claims abstract description 33
- 239000011521 glass Substances 0.000 claims abstract description 33
- 238000002791 soaking Methods 0.000 claims abstract description 25
- 238000001035 drying Methods 0.000 claims abstract description 23
- 229920006267 polyester film Polymers 0.000 claims abstract description 23
- 239000010931 gold Substances 0.000 claims abstract description 17
- 229910052737 gold Inorganic materials 0.000 claims abstract description 17
- 238000005507 spraying Methods 0.000 claims abstract description 17
- 238000002372 labelling Methods 0.000 claims abstract description 13
- 229920000728 polyester Polymers 0.000 claims abstract description 12
- 238000007789 sealing Methods 0.000 claims abstract description 8
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 18
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 18
- 238000000034 method Methods 0.000 claims description 10
- 229920001213 Polysorbate 20 Polymers 0.000 claims description 9
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 9
- 229930006000 Sucrose Natural products 0.000 claims description 9
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims description 9
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 9
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 9
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 9
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 claims description 9
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 claims description 9
- 239000001103 potassium chloride Substances 0.000 claims description 9
- 235000011164 potassium chloride Nutrition 0.000 claims description 9
- 239000011780 sodium chloride Substances 0.000 claims description 9
- 239000005720 sucrose Substances 0.000 claims description 9
- 229910021642 ultra pure water Inorganic materials 0.000 claims description 9
- 239000012498 ultrapure water Substances 0.000 claims description 9
- 239000000463 material Substances 0.000 claims description 7
- 238000005119 centrifugation Methods 0.000 claims description 6
- 239000002245 particle Substances 0.000 abstract description 5
- 239000000126 substance Substances 0.000 abstract description 3
- 239000000758 substrate Substances 0.000 abstract description 3
- 238000003556 assay Methods 0.000 abstract 1
- 238000001514 detection method Methods 0.000 description 7
- 238000005516 engineering process Methods 0.000 description 3
- 238000003317 immunochromatography Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 239000002105 nanoparticle Substances 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 229920000298 Cellophane Polymers 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 230000036046 immunoreaction Effects 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000010079 rubber tapping Methods 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
- G01N33/587—Nanoparticles
Abstract
The invention relates to the technical field of immunochromatographic assay, and discloses a preparation method of a colloidal gold immunochromatographic test strip, which comprises the following steps: respectively soaking the glass cellulose membrane and the polyester membrane in a pretreatment soaking solution, and drying; sealing, centrifuging and concentrating the marked monoclonal antibody, spraying the concentrated colloidal gold solution on a polyester film, drying and preparing a gold-labeled conjugate pad; and assembling the gold-labeled binding pad and the glass cellulose membrane into the colloidal gold immunochromatographic test strip. The pretreatment soaking solution can effectively remove the substrates in the commercial gold label pad and the sample pad, and improve the physical and chemical properties of the gold label pad and the sample pad; the test strip treated by the pretreatment soaking solution of the invention has the advantages of uniform labeling and release of colloidal gold particles and greatly improved stability of gold-labeled conjugates.
Description
Technical Field
The invention relates to the technical field of immunochromatography detection, in particular to a preparation method of a colloidal gold immunochromatography test strip.
Background
The colloidal gold immunochromatography technology is a technology which combines antigen-antibody immunoreaction with a colloidal gold labeling tracing technology and is used for qualitative and quantitative detection of the content of antigen and antibody. The colloidal gold labeling of the antibody is mainly firmly combined through the electrostatic acting force between the colloidal gold and the protein, and particularly, a brand-new gate is opened for the on-site rapid detection and tapping due to the advantages of rapidness, simplicity, convenience, low cost, good stability and the like.
The gold-labeled pad and the sample pad are important components of the colloidal gold immunochromatographic test paper, and the pretreatment of the gold-labeled pad and the sample pad has different degrees of influence on subsequent detection. The gold label pad has the main function of adsorbing the gold nanoparticles marked with corresponding protein antibodies, and determines the sensitivity, specificity, release effect and the like of the subsequent gold nanoparticles. Most of the existing labeling methods of gold-labeled pads are to spray labeled concentrated gold particles on a glass cellulose membrane, and the labeling amount of the gold-labeled combined pad prepared by the method is small, the gold-labeled combined pad is incompletely released in the detection process, and the accuracy and the sensitivity of the detection result are influenced. The sample pad is also pretreated before use, the matrix in the sample pad has great influence, and when the sample pad is directly applied to detection without treatment, great problems exist, such as: false positive and false negative reaction, low negative-positive contrast, and ineffective promotion of gold particle release in the gold-labeled conjugate pad.
Disclosure of Invention
In order to solve the technical problems, the invention provides a preparation method of a colloidal gold immunochromatographic test strip.
The specific technical scheme of the invention is as follows: a preparation method of a colloidal gold immunochromatographic test strip comprises the following steps:
1) selecting materials: selecting glass cellulose membrane and polyester film, and storing in shade.
The aging of the cellophane and polyester films is due to the evaporation of water from the films, which makes the films hydrophobic, charged and brittle. Storage films are generally required to be sealed from light and are disadvantageous in terms of being too dry or too wet.
2) Soaking: adding the pretreatment soaking solution into a container, and respectively immersing the glass cellulose membrane and the polyester membrane for 25-35 s.
The glass cellulose membrane and the polyester membrane are pretreated to increase the water absorption rate, remove the charge influence on the substrate and ensure the functional uniformity of the membrane.
3) Drying: and respectively transferring the soaked glass cellulose membrane and the polyester membrane to 35-40 ℃ for drying.
4) Preparing a gold-labeled bonding pad: labeling the corresponding monoclonal antibody with the prepared colloidal gold solution, sealing, centrifuging and concentrating the labeled monoclonal antibody to obtain a concentrated colloidal gold solution, spraying the concentrated colloidal gold solution on the polyester film obtained in the step 3) by using a gold spraying and scribing machine, and drying at 35-40 ℃ to prepare the gold-labeled conjugate pad.
And spraying the marked colloidal gold on the polyester film by using a gold spraying and scribing machine to form a uniform gold-labeled bonding pad on the polyester film.
5) Assembling: assembling the gold-labeled binding pad and the glass cellulose membrane obtained in the step 3) into the colloidal gold immunochromatographic test strip.
Preferably, in the step 1), the pretreatment soaking solution comprises the following components in percentage by mass:
disodium hydrogen phosphate: 0.142-0.363%, potassium dihydrogen phosphate: 0.024% -0.027%, sodium chloride: 0.8%, potassium chloride: 0.02%, BSA: 1%, sucrose: 1 percent of Tween-20:0.2 percent, and is dissolved in ultrapure water.
Preferably, in the step 1), the pretreatment soaking solution comprises the following components in percentage by mass:
disodium hydrogen phosphate: 0.363%, potassium dihydrogen phosphate: 0.024%, sodium chloride: 0.8%, potassium chloride: 0.02%, BSA: 1%, sucrose: 1 percent of Tween-20:0.2 percent, and is dissolved in ultrapure water.
Preferably, in the step 4), the centrifugation condition is 10000-15000 rpm, and the centrifugation time is 15-30 min.
Further preferably, the centrifugation condition is 12000rpm, and the centrifugation time is 30 min.
Preferably, in the step 4), the concentrated colloidal gold solution is 1/10 with the original concentration.
Compared with the prior art, the invention has the beneficial effects that:
1) the pretreatment soaking solution can effectively remove the substrates in the commercial gold label pad and the sample pad, and improve the physical and chemical properties of the gold label pad and the sample pad;
2) the test strip obtained after the pretreatment soaking solution is treated by the method has uniform labeling and release of the colloidal gold particles, and the stability of the gold-labeled conjugate is greatly improved;
3) the sample pad treated by the pretreatment soaking solution is uniformly absorbed, and the influence of the physical and chemical properties of the sample pad is reduced.
Drawings
FIG. 1 is a comparison of test results of different colloidal gold immunochromatographic test strips.
Detailed Description
The present invention will be further described with reference to the following examples.
Example 1
A preparation method of a colloidal gold immunochromatographic test strip comprises the following steps:
1) selecting materials: selecting glass cellulose membrane and polyester film, and storing in shade.
2) Soaking: the pretreatment soak was added to the vessel and the glass cellulose film and polyester film were immersed for 30 seconds, respectively. The pretreatment soaking solution comprises the following components in percentage by mass: disodium hydrogen phosphate: 0.363%, potassium dihydrogen phosphate: 0.024%, sodium chloride: 0.8%, potassium chloride: 0.02%, BSA: 1%, sucrose: 1 percent of Tween-20:0.2 percent, and is dissolved in ultrapure water.
3) Drying: and respectively transferring the soaked glass cellulose membrane and the polyester membrane to 37 ℃ for drying.
4) Preparing a gold-labeled bonding pad: labeling the corresponding monoclonal antibody with the prepared colloidal gold solution, sealing the labeled monoclonal antibody, centrifuging (15000 rpm, 30 min), concentrating to obtain a concentrated colloidal gold solution, spraying the concentrated colloidal gold solution on the polyester film obtained in the step 3) by using a gold spraying and scribing machine, and drying at 37 ℃ to prepare the gold-labeled conjugate pad.
5) Assembling: assembling the gold-labeled binding pad and the glass cellulose membrane obtained in the step 3) into the colloidal gold immunochromatographic test strip.
FIG. 1 (left) is a colloidal gold immunochromatographic test strip obtained in example 1 of the present invention, colloidal gold particles prepared by the method are labeled and released uniformly, the color development on the membrane is clear and obvious, and the stability of the gold-labeled conjugate is greatly improved; the colloidal gold in the figures (middle and right) is unevenly distributed on the membrane, and has different color depth and poor effect.
Example 2
A preparation method of a colloidal gold immunochromatographic test strip comprises the following steps:
1) selecting materials: selecting glass cellulose membrane and polyester film, and storing in shade.
2) Soaking: the pretreatment soak was added to the vessel and the glass cellulose film and polyester film were immersed for 30 seconds, respectively. The pretreatment soaking solution comprises the following components in percentage by mass: disodium hydrogen phosphate: 0.142%, potassium dihydrogen phosphate: 0.027%, sodium chloride: 0.8%, potassium chloride: 0.02%, BSA: 1%, sucrose: 1 percent of Tween-20:0.2 percent, and is dissolved in ultrapure water.
3) Drying: and respectively transferring the soaked glass cellulose membrane and the polyester membrane to 37 ℃ for drying.
4) Preparing a gold-labeled bonding pad: labeling the corresponding monoclonal antibody with the prepared colloidal gold solution, sealing the labeled monoclonal antibody, centrifuging (15000 rpm, 30 min), concentrating to obtain a concentrated colloidal gold solution, spraying the concentrated colloidal gold solution on the polyester film obtained in the step 3) by using a gold spraying and scribing machine, and drying at 37 ℃ to prepare the gold-labeled conjugate pad.
5) Assembling: assembling the gold-labeled binding pad and the glass cellulose membrane obtained in the step 3) into the colloidal gold immunochromatographic test strip.
Example 3
A preparation method of a colloidal gold immunochromatographic test strip comprises the following steps:
1) selecting materials: selecting glass cellulose membrane and polyester film, and storing in shade.
2) Soaking: the pretreatment soak was added to the vessel and the glass cellulose film and polyester film were immersed for 30 seconds, respectively. The pretreatment soaking solution comprises the following components in percentage by mass: disodium hydrogen phosphate: 0.363%, potassium dihydrogen phosphate: 0.024%, sodium chloride: 0.8%, potassium chloride: 0.02%, BSA: 1%, sucrose: 1 percent of Tween-20:0.2 percent, and is dissolved in ultrapure water.
3) Drying: and respectively transferring the soaked glass cellulose membrane and the polyester membrane to 37 ℃ for drying.
4) Preparing a gold-labeled bonding pad: labeling the corresponding monoclonal antibody with the prepared colloidal gold solution, sealing the labeled monoclonal antibody, centrifuging (15000 rpm, 30 min), concentrating to obtain a concentrated colloidal gold solution, spraying the concentrated colloidal gold solution on the polyester film obtained in the step 3) by using a gold spraying and scribing machine, and drying at 37 ℃ to prepare the gold-labeled conjugate pad.
5) Assembling: assembling the gold-labeled binding pad and the glass cellulose membrane obtained in the step 3) into the colloidal gold immunochromatographic test strip.
Example 4
A preparation method of a colloidal gold immunochromatographic test strip comprises the following steps:
1) selecting materials: selecting glass cellulose membrane and polyester film, and storing in shade.
2) Soaking: the pretreatment soak was added to the vessel and the glass cellulose film and polyester film were immersed for 30 seconds, respectively. The pretreatment soaking solution comprises the following components in percentage by mass: disodium hydrogen phosphate: 0.363%, potassium dihydrogen phosphate: 0.024%, sodium chloride: 0.8%, potassium chloride: 0.02%, BSA: 1%, sucrose: 1 percent of Tween-20:0.2 percent, and is dissolved in ultrapure water.
3) Drying: and respectively transferring the soaked glass cellulose membrane and the polyester membrane to 37 ℃ for drying.
4) Preparing a gold-labeled bonding pad: labeling the corresponding monoclonal antibody with the prepared colloidal gold solution, sealing the labeled monoclonal antibody, centrifuging (12000 rpm for 15 min), concentrating to obtain a concentrated colloidal gold solution, spraying the concentrated colloidal gold solution on the polyester film obtained in the step 3) by using a gold spraying and scribing machine, and drying at 37 ℃ to prepare the gold-labeled conjugate pad.
5) Assembling: assembling the gold-labeled binding pad and the glass cellulose membrane obtained in the step 3) into the colloidal gold immunochromatographic test strip.
Example 5
A preparation method of a colloidal gold immunochromatographic test strip comprises the following steps:
1) selecting materials: selecting glass cellulose membrane and polyester film, and storing in shade.
2) Soaking: the pretreatment soak was added to the vessel and the glass cellulose film and polyester film were immersed for 30 seconds, respectively. The pretreatment soaking solution comprises the following components in percentage by mass: disodium hydrogen phosphate: 0.142%, potassium dihydrogen phosphate: 0.027%, sodium chloride: 0.8%, potassium chloride: 0.02%, BSA: 1%, sucrose: 1 percent of Tween-20:0.2 percent, and is dissolved in ultrapure water.
3) Drying: and respectively transferring the soaked glass cellulose membrane and the polyester membrane to 37 ℃ for drying.
4) Preparing a gold-labeled bonding pad: labeling the corresponding monoclonal antibody with the prepared colloidal gold solution, sealing the labeled monoclonal antibody, centrifuging (12000 rpm for 15 min), concentrating to obtain a concentrated colloidal gold solution, spraying the concentrated colloidal gold solution on the polyester film obtained in the step 3) by using a gold spraying and scribing machine, and drying at 37 ℃ to prepare the gold-labeled conjugate pad.
5) Assembling: assembling the gold-labeled binding pad and the glass cellulose membrane obtained in the step 3) into the colloidal gold immunochromatographic test strip.
The raw materials and equipment used in the invention are common raw materials and equipment in the field if not specified; the methods used in the present invention are conventional in the art unless otherwise specified.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention, and all simple modifications, alterations and equivalents of the above embodiments according to the technical spirit of the present invention are still within the protection scope of the technical solution of the present invention.
Claims (6)
1. A preparation method of a colloidal gold immunochromatographic test strip is characterized by comprising the following steps:
1) selecting materials: selecting a glass cellulose membrane and a polyester membrane, and storing the glass cellulose membrane and the polyester membrane in a shady and cool place away from light;
2) soaking: adding the pretreatment soaking solution into a container, and respectively immersing the glass cellulose membrane and the polyester membrane for 25-35 s;
3) drying: respectively transferring the soaked glass cellulose membrane and polyester film to 35-40 ℃ for drying;
4) preparing a gold-labeled bonding pad: labeling corresponding monoclonal antibodies with the prepared colloidal gold solution, sealing, centrifuging and concentrating the labeled monoclonal antibodies to obtain a concentrated colloidal gold solution, spraying the concentrated colloidal gold solution on the polyester film obtained in the step 3) by using a gold spraying and scribing machine, and drying at 35-40 ℃ to prepare a gold-labeled conjugate pad;
5) assembling: assembling the gold-labeled binding pad and the glass cellulose membrane obtained in the step 3) into the colloidal gold immunochromatographic test strip.
2. The method of claim 1, wherein: in the step 1), the pretreatment soaking solution comprises the following components in percentage by mass:
disodium hydrogen phosphate: 0.142-0.363%, potassium dihydrogen phosphate: 0.024% -0.027%, sodium chloride: 0.8%, potassium chloride: 0.02%, BSA: 1%, sucrose: 1 percent of Tween-20:0.2 percent, and is dissolved in ultrapure water.
3. The method of claim 2, wherein: in the step 1), the pretreatment soaking solution comprises the following components in percentage by mass:
disodium hydrogen phosphate: 0.363%, potassium dihydrogen phosphate: 0.024%, sodium chloride: 0.8%, potassium chloride: 0.02%, BSA: 1%, sucrose: 1 percent of Tween-20:0.2 percent, and is dissolved in ultrapure water.
4. The method of claim 1, wherein: in the step 4), the centrifugation condition is 10000-15000 rpm, and the centrifugation time is 15-30 min.
5. The method of claim 1, wherein: in the step 4), the centrifugal condition is the rotating speed of 12000rpm, and the centrifugal time is 30 min.
6. The method of claim 1, wherein: in the step 4), the concentrated colloidal gold solution is 1/10 with the original concentration.
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| CN104880552A (en) * | 2015-05-20 | 2015-09-02 | 集美大学 | Colloidal gold immunochromatography test strip for detecting enrofloxacin and preparation method of colloidal gold immunochromatography test strip |
| CN108226487A (en) * | 2016-12-22 | 2018-06-29 | 中粮集团有限公司 | Zearalenone detection card and the zearalenone detection method blocked using the detection |
| CN108956992A (en) * | 2018-05-02 | 2018-12-07 | 北京厚生正德科技有限公司 | A kind of preparation method of the Ribavirin test strip based on quantum dot fluorescence |
| CN111638327A (en) * | 2020-07-01 | 2020-09-08 | 成都赛普克生物科技股份有限公司 | Colloidal gold immunochromatographic test paper gold label pad, treatment solution for sample pad and treatment method |
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2021
- 2021-03-29 CN CN202110331518.3A patent/CN113009129A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104880552A (en) * | 2015-05-20 | 2015-09-02 | 集美大学 | Colloidal gold immunochromatography test strip for detecting enrofloxacin and preparation method of colloidal gold immunochromatography test strip |
| CN108226487A (en) * | 2016-12-22 | 2018-06-29 | 中粮集团有限公司 | Zearalenone detection card and the zearalenone detection method blocked using the detection |
| CN108956992A (en) * | 2018-05-02 | 2018-12-07 | 北京厚生正德科技有限公司 | A kind of preparation method of the Ribavirin test strip based on quantum dot fluorescence |
| CN111638327A (en) * | 2020-07-01 | 2020-09-08 | 成都赛普克生物科技股份有限公司 | Colloidal gold immunochromatographic test paper gold label pad, treatment solution for sample pad and treatment method |
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