CN112998954A - Preparation method of glycerin skin dressing for treating chronic skin ulcer - Google Patents
Preparation method of glycerin skin dressing for treating chronic skin ulcer Download PDFInfo
- Publication number
- CN112998954A CN112998954A CN202110218846.2A CN202110218846A CN112998954A CN 112998954 A CN112998954 A CN 112998954A CN 202110218846 A CN202110218846 A CN 202110218846A CN 112998954 A CN112998954 A CN 112998954A
- Authority
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- China
- Prior art keywords
- skin
- glycerin
- physiological saline
- preparation
- cleaning
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 title claims abstract description 81
- 235000011187 glycerol Nutrition 0.000 title claims abstract description 34
- 238000002360 preparation method Methods 0.000 title claims abstract description 16
- 206010040943 Skin Ulcer Diseases 0.000 title claims abstract description 9
- 239000010985 leather Substances 0.000 claims abstract description 31
- 238000004140 cleaning Methods 0.000 claims abstract description 29
- 239000002504 physiological saline solution Substances 0.000 claims abstract description 29
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 12
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 12
- 238000007789 sealing Methods 0.000 claims abstract description 10
- 238000004806 packaging method and process Methods 0.000 claims abstract description 8
- 230000001954 sterilising effect Effects 0.000 claims abstract description 7
- 238000005520 cutting process Methods 0.000 claims abstract description 6
- 238000003756 stirring Methods 0.000 claims abstract description 6
- 238000002791 soaking Methods 0.000 claims abstract description 5
- 229910001220 stainless steel Inorganic materials 0.000 claims description 26
- 239000010935 stainless steel Substances 0.000 claims description 26
- 208000025865 Ulcer Diseases 0.000 claims description 8
- 230000001684 chronic effect Effects 0.000 claims description 8
- 231100000397 ulcer Toxicity 0.000 claims description 8
- 239000011521 glass Substances 0.000 claims description 6
- 230000000694 effects Effects 0.000 claims description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 8
- 206010052428 Wound Diseases 0.000 description 5
- 208000027418 Wounds and injury Diseases 0.000 description 5
- 206010072170 Skin wound Diseases 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 230000000735 allogeneic effect Effects 0.000 description 2
- 201000006994 chronic ulcer of skin Diseases 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 230000035876 healing Effects 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 238000009455 aseptic packaging Methods 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 231100000241 scar Toxicity 0.000 description 1
- 230000008591 skin barrier function Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F13/00—Bandages or dressings; Absorbent pads
- A61F13/02—Adhesive bandages or dressings
- A61F13/0259—Adhesive bandages or dressings characterised by the release liner covering the skin adhering layer
- A61F13/0266—Adhesive bandages or dressings characterised by the release liner covering the skin adhering layer especially adapted for wound covering/occlusive dressings
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F13/00—Bandages or dressings; Absorbent pads
- A61F13/02—Adhesive bandages or dressings
- A61F13/0259—Adhesive bandages or dressings characterised by the release liner covering the skin adhering layer
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F13/00—Bandages or dressings; Absorbent pads
- A61F13/02—Adhesive bandages or dressings
- A61F13/0276—Apparatus or processes for manufacturing adhesive dressings or bandages
- A61F13/0289—Apparatus or processes for manufacturing adhesive dressings or bandages manufacturing of adhesive dressings
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
- A61F2/02—Prostheses implantable into the body
- A61F2/10—Hair or skin implants
- A61F2/105—Skin implants, e.g. artificial skin
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Heart & Thoracic Surgery (AREA)
- Vascular Medicine (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Biomedical Technology (AREA)
- Dermatology (AREA)
- Transplantation (AREA)
- Cardiology (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Manufacturing & Machinery (AREA)
- Materials For Medical Uses (AREA)
Abstract
The invention discloses a preparation method of a glycerin skin dressing for treating chronic skin ulcer, which comprises the following steps: obtaining qualified donor skin and carrying out disinfection treatment; adding the obtained skin pieces into physiological saline, stirring, and cleaning for multiple times; adding the cleaned skin pieces into medical alcohol, and sealing and soaking; adding the soaked skin pieces into a glycerol physiological saline solution with the concentration of 50%, standing at normal temperature for a certain time, and performing primary glycerolysis treatment; adding the primarily glycerinated skin pieces into a glycerin physiological saline solution with the concentration of 85%, and performing secondary glycerination treatment by using a constant-temperature shaking table; cutting the leather sheets after twice glycerination into samples with different specifications, sealing and packaging, and then performing irradiation sterilization. The present invention treats allogenic skin through combination of glycerin treatment and irradiation treatment, and can be stored and transported aseptically at normal temperature.
Description
Technical Field
The invention relates to the technical field of biological materials, in particular to a preparation method of a glycerin skin dressing for treating chronic skin ulcer.
Background
The skin is the largest organ of the human body, is an important barrier for protecting the body from radiation in the external environment and direct harm of chemical substances, bacteria, viruses and the like, and has the functions of preventing the loss of water and electrolyte in the body and regulating the balance of water and other substances in the body. Chronic ulcer of skin caused by various reasons is not easy to heal, the chronic ulcer is not easy to heal by conventional dressing change or autologous skin transplantation, and scar cancer can be caused after long-term healing.
Therefore, the dressing is adopted to cover the wound surface, so that the protective effect on the skin wound surface can be achieved, a favorable warm and moist environment is provided for the wound surface, the wound surface healing is promoted, and the wound surface is waited to be epithelialized or matched with skin grafting to transit to a reconstructed permanent skin barrier, so that the wound surface is cured.
The conventional allogeneic skin has good effect on clinically treating skin wound, but has the defects of inconvenient storage and transportation and high cost.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide a preparation method of a glycerin skin dressing for treating chronic ulcer of skin, which treats allogeneic skin by glycerin and irradiation in a combined way and can be stored and transported aseptically at normal temperature.
The technical scheme adopted by the invention for realizing the purpose is as follows: a preparation method of a glycerin skin dressing for treating chronic skin ulcer comprises the following steps:
s1: obtaining qualified donor skin and carrying out disinfection treatment;
s2: adding the skin sheets obtained in the step S1 into physiological saline, stirring, and cleaning for multiple times;
s3: adding the skin pieces processed in the step S2 into medical alcohol for sealing and soaking;
s4: adding the skin pieces treated in the step S3 into a glycerin physiological saline solution with the concentration of 50%, standing for a specific time at normal temperature for glycerination;
s5: adding the skin pieces treated in the step S4 into a glycerol physiological saline solution with the concentration of 85%, and performing glycerolysis treatment by using a constant-temperature shaking table;
s6: and cutting the leather sheets processed in the step S5 into samples with different specifications, sealing and packaging the samples, and then performing irradiation sterilization.
In the step S1, when the skin is taken, the thickness range of the skin sheet is 0.2-0.8 mm, and the effect is best when the skin sheet with the thickness of 0.4mm is adopted.
When the leather sheets are cleaned in the step S2, the leather sheets are firstly put into a sterile stainless steel tank, physiological saline is added and stirred by a sterile glass rod for cleaning for a plurality of times, and the physiological saline is replaced again for cleaning the next time after the cleaning is finished each time.
When the leather sheet is processed in the step S3, firstly, the leather sheet is added into a stainless steel groove, medical alcohol is injected into a sterile stainless steel groove until the leather sheet is immersed, the cover is covered, the seal is sealed and the medical alcohol in the sterile stainless steel groove is removed after the leather sheet is immersed, physiological saline is added and stirred by a sterile glass rod for cleaning for multiple times, and the physiological saline is replaced again for cleaning the next time after the cleaning is finished each time.
And S4, when the leather sheets are processed, placing the leather sheets in a sterile stainless steel tank, adding a glycerol physiological saline solution with the concentration of 50%, and standing for 2-5 hours at normal temperature for glycerolysis.
In the step S5, when the skin sheet is processed, the skin sheet is drained and then placed in a sterile stainless steel tank, a glycerol physiological saline solution with a concentration of 85% is added, and the sterile stainless steel tank is placed in a constant temperature shaking table for glycerolysis.
In the step S6, when the skin piece is cut, the cut specification includes a rectangular shape of 5cm × 5cm, 10cm × 10cm, 5cm × 10cm, 10cm × 20cm, 20cm × 20cm, or the like.
In the step S6, when the skin sheets are sealed and packaged, the packaging bag is an aseptic bag with at least two sealed layers.
The invention has the beneficial effects that: the skin sheet is prepared from the qualified donor skin, the qualified skin sheet is prepared after sterilization, multiple cleaning and glycerolysis treatment, irradiation sterilization is carried out after aseptic packaging, aseptic storage and transportation can be carried out at normal temperature, the water activity of the skin sheet can be ensured for a long time, and the requirement of timely supplying a large amount of biological dressings required by skin wound treatment under various conditions is met.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
A preparation method of a glycerin skin dressing for treating chronic skin ulcer comprises the following steps:
s1: obtaining qualified donor skin and carrying out disinfection treatment;
s2: adding the skin sheets obtained in the step S1 into physiological saline, stirring, and cleaning for multiple times;
s3: adding the skin pieces processed in the step S2 into medical alcohol for sealing and soaking;
s4: adding the skin pieces treated in the step S3 into a glycerin physiological saline solution with the concentration of 50%, standing for a specific time at normal temperature for glycerination;
s5: adding the skin pieces treated in the step S4 into a glycerol physiological saline solution with the concentration of 85%, and performing glycerolysis treatment by using a constant-temperature shaking table;
s6: and cutting the leather sheets processed in the step S5 into samples with different specifications, sealing and packaging the samples, and then performing irradiation sterilization.
Example 2
Further supplementary to example 1, in step S1, when skin is taken, the thickness of the skin sheet is in the range of 0.2-0.8 mm, and the effect is best when the skin sheet with the thickness of 0.4mm is used.
Step S2 is that when cleaning the leather sheet, firstly, the leather sheet is put into a sterile stainless steel tank, normal saline is added and stirred by a sterile glass rod for cleaning for a plurality of times, and the normal saline is replaced again for cleaning the next time after cleaning each time.
Step S3, when the leather sheet is processed, firstly, the leather sheet is added into a stainless steel groove, medical alcohol is injected into a sterile stainless steel groove until the leather sheet is immersed, after the leather sheet is covered, sealed and soaked, the medical alcohol in the sterile stainless steel groove is removed, normal saline is added and stirred by a sterile glass rod for cleaning for many times, and the normal saline is replaced again for cleaning the next time after the cleaning is finished each time.
Step S4, when the leather sheet is processed, the leather sheet is placed in a sterile stainless steel tank, a glycerol physiological saline solution with the concentration of 50% is added, and standing is carried out for 2-5 hours at normal temperature for glycerolysis.
Step S5, when the skin is treated, the skin is placed in a sterile stainless steel tank after being drained, a glycerol physiological saline solution with the concentration of 85% is added, and the sterile stainless steel tank is placed in a constant temperature shaking table for glycerolysis treatment.
In the step S6, when the skin sheet is cut, the cut specification includes a rectangular shape of 5cm × 5cm, 10cm × 10cm, 5cm × 10cm, 10cm × 20cm, 20cm × 20cm, or the like.
In step S6, when the skin sheet is sealed and packaged, the packaging bag is an aseptic bag with at least two sealed layers.
Example 3
The preparation procedure provided in example 1 was followed to prepare a glycerinated skin dressing:
s1: taking fresh qualified skin, wiping with iodophor for disinfection, and removing skin with a skin drum machine to obtain 0.4mm skin slice;
s2: placing the skin pieces in a sterile stainless steel tank, adding normal saline, stirring and cleaning for multiple times, and replacing the normal saline after each cleaning for the next cleaning;
s3: placing the cleaned skin sheet in a sterile stainless steel tank, adding medical alcohol with the concentration of 70% to submerge the skin sheet, sealing and soaking the skin sheet for a certain period of time by a cover, removing the alcohol, adding normal saline, stirring and cleaning for multiple times, and replacing the normal saline after each cleaning to clean the next time;
s4: placing the leather sheets processed in the step S3 in a sterile stainless steel tank, adding a glycerol physiological saline solution with the concentration of 50%, and standing at normal temperature for primary glycerolysis treatment;
s5: draining off the glycerol physiological saline solution on the primarily glycerolized skin pieces, placing the primarily glycerolized skin pieces in an aseptic stainless steel tank, adding the glycerol physiological saline solution with the concentration of 85%, and placing the aseptic stainless steel tank in a constant temperature shaking table for secondary glycerolization;
s6: cutting the leather sheet subjected to the secondary glycerolysis treatment into a rectangular shape of 10 x 10cm, sealing and packaging, and performing irradiation sterilization to generate a qualified leather sheet.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (8)
1. A preparation method of a glycerin skin dressing for treating chronic skin ulcer is characterized by comprising the following steps:
s1: obtaining qualified donor skin and carrying out disinfection treatment;
s2: adding the skin sheets obtained in the step S1 into physiological saline, stirring, and cleaning for multiple times;
s3: adding the skin pieces processed in the step S2 into medical alcohol for sealing and soaking;
s4: adding the skin pieces treated in the step S3 into a glycerin physiological saline solution with the concentration of 50%, standing for a specific time at normal temperature for glycerination;
s5: adding the skin pieces treated in the step S4 into a glycerol physiological saline solution with the concentration of 85%, and performing glycerolysis treatment by using a constant-temperature shaking table;
s6: and cutting the leather sheets processed in the step S5 into samples with different specifications, sealing and packaging the samples, and then performing irradiation sterilization.
2. The preparation method of the glycerin skin dressing for treating the skin chronic ulcer according to the claim 1, characterized in that: in the step S1, when the skin is taken, the thickness range of the skin sheet is 0.2-0.8 mm, and the effect is best when the skin sheet with the thickness of 0.4mm is adopted.
3. The preparation method of the glycerin skin dressing for treating the skin chronic ulcer according to the claim 1, characterized in that: when the leather sheets are cleaned in the step S2, the leather sheets are firstly put into a sterile stainless steel tank, physiological saline is added and stirred by a sterile glass rod for cleaning for a plurality of times, and the physiological saline is replaced again for cleaning the next time after the cleaning is finished each time.
4. The preparation method of the glycerin skin dressing for treating the skin chronic ulcer according to the claim 1, characterized in that: when the leather sheet is processed in the step S3, firstly, the leather sheet is added into a stainless steel groove, medical alcohol is injected into a sterile stainless steel groove until the leather sheet is immersed, the cover is covered, the seal is sealed and the medical alcohol in the sterile stainless steel groove is removed after the leather sheet is immersed, physiological saline is added and stirred by a sterile glass rod for cleaning for multiple times, and the physiological saline is replaced again for cleaning the next time after the cleaning is finished each time.
5. The preparation method of the glycerin skin dressing for treating the skin chronic ulcer according to the claim 1, characterized in that: and S4, when the leather sheets are processed, placing the leather sheets in a sterile stainless steel tank, adding a glycerol physiological saline solution with the concentration of 50%, and standing for 2-5 hours at normal temperature for glycerolysis.
6. The preparation method of the glycerin skin dressing for treating the skin chronic ulcer according to the claim 1, characterized in that: in the step S5, when the skin sheet is processed, the skin sheet is drained and then placed in a sterile stainless steel tank, a glycerol physiological saline solution with a concentration of 85% is added, and the sterile stainless steel tank is placed in a constant temperature shaking table for glycerolysis.
7. The preparation method of the glycerin skin dressing for treating the skin chronic ulcer according to the claim 1, characterized in that: in the step S6, when the skin piece is cut, the cutting specification includes a rectangular shape of 5cm × 5cm, 10cm × 10cm, 5cm × 10cm, 10cm × 20cm, and 20cm × 20 cm.
8. The preparation method of the glycerin skin dressing for treating the skin chronic ulcer according to the claim 1, characterized in that: in the step S6, when the skin sheets are sealed and packaged, the packaging bag is an aseptic bag with at least two sealed layers.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110218846.2A CN112998954A (en) | 2021-02-26 | 2021-02-26 | Preparation method of glycerin skin dressing for treating chronic skin ulcer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110218846.2A CN112998954A (en) | 2021-02-26 | 2021-02-26 | Preparation method of glycerin skin dressing for treating chronic skin ulcer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112998954A true CN112998954A (en) | 2021-06-22 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110218846.2A Pending CN112998954A (en) | 2021-02-26 | 2021-02-26 | Preparation method of glycerin skin dressing for treating chronic skin ulcer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112998954A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011065672A2 (en) * | 2009-11-24 | 2011-06-03 | Kim Tae Woon | Method for producing medical dressing using pig skin |
| CN108079364A (en) * | 2018-01-18 | 2018-05-29 | 李峰 | A kind of frog skin burn wound biological dressing preparation method |
| CN109381731A (en) * | 2017-08-02 | 2019-02-26 | 长沙海润生物技术有限公司 | A kind of medical bio bressing and preparation method thereof |
-
2021
- 2021-02-26 CN CN202110218846.2A patent/CN112998954A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2011065672A2 (en) * | 2009-11-24 | 2011-06-03 | Kim Tae Woon | Method for producing medical dressing using pig skin |
| CN109381731A (en) * | 2017-08-02 | 2019-02-26 | 长沙海润生物技术有限公司 | A kind of medical bio bressing and preparation method thereof |
| CN108079364A (en) * | 2018-01-18 | 2018-05-29 | 李峰 | A kind of frog skin burn wound biological dressing preparation method |
Non-Patent Citations (1)
| Title |
|---|
| 黄跃生；刘德培: "《中华医学百科全书 临床医学 烧伤外科学》", 31 March 2017, 中国协和医科大学出版社 * |
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| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210622 |
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