CN112986444A - Target molecule detection method based on molecularly imprinted polymer - Google Patents
Target molecule detection method based on molecularly imprinted polymer Download PDFInfo
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- 229920000344 molecularly imprinted polymer Polymers 0.000 title claims abstract description 57
- 238000001514 detection method Methods 0.000 title claims abstract description 52
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 64
- 238000002414 normal-phase solid-phase extraction Methods 0.000 claims abstract description 51
- 238000000034 method Methods 0.000 claims abstract description 27
- 238000011049 filling Methods 0.000 claims abstract description 6
- 238000005086 pumping Methods 0.000 claims abstract description 4
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 claims description 51
- 239000008367 deionised water Substances 0.000 claims description 13
- 229910021641 deionized water Inorganic materials 0.000 claims description 13
- 239000012535 impurity Substances 0.000 claims description 10
- -1 polypropylene Polymers 0.000 claims description 8
- 230000003213 activating effect Effects 0.000 claims description 7
- 238000011068 loading method Methods 0.000 claims description 7
- 230000008569 process Effects 0.000 claims description 7
- 238000002386 leaching Methods 0.000 claims description 6
- 238000010828 elution Methods 0.000 claims description 5
- 239000007788 liquid Substances 0.000 claims description 5
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 claims description 4
- 229920000642 polymer Polymers 0.000 claims description 3
- 239000004743 Polypropylene Substances 0.000 claims description 2
- 229920001155 polypropylene Polymers 0.000 claims description 2
- 229920001343 polytetrafluoroethylene Polymers 0.000 claims description 2
- 239000004810 polytetrafluoroethylene Substances 0.000 claims description 2
- 239000000523 sample Substances 0.000 description 26
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 238000010586 diagram Methods 0.000 description 4
- 230000008859 change Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000001179 sorption measurement Methods 0.000 description 3
- IISBACLAFKSPIT-UHFFFAOYSA-N bisphenol A Chemical compound C=1C=C(O)C=CC=1C(C)(C)C1=CC=C(O)C=C1 IISBACLAFKSPIT-UHFFFAOYSA-N 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 229910052739 hydrogen Inorganic materials 0.000 description 2
- 239000001257 hydrogen Substances 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 238000012123 point-of-care testing Methods 0.000 description 2
- 230000002378 acidificating effect Effects 0.000 description 1
- QXJJQWWVWRCVQT-UHFFFAOYSA-K calcium;sodium;phosphate Chemical compound [Na+].[Ca+2].[O-]P([O-])([O-])=O QXJJQWWVWRCVQT-UHFFFAOYSA-K 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 238000002290 gas chromatography-mass spectrometry Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000004895 liquid chromatography mass spectrometry Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 150000003071 polychlorinated biphenyls Chemical class 0.000 description 1
- 230000005588 protonation Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
Abstract
The invention discloses a target molecule detection method based on a molecularly imprinted polymer, which comprises the following steps of firstly, taking a specific molecularly imprinted polymer as an identification unit, and filling the specific molecularly imprinted polymer into a solid phase extraction column; the upper part of the solid phase extraction column is connected with a container for containing water, and the lower part of the solid phase extraction column is loaded on a solid phase extraction instrument; connecting the solid phase extraction column with a vacuum pump for vacuum pumping to enable water on the upper part to flow rapidly; and recording the time when the water completely passes through the solid phase extraction column by using a timer, and converting the molecular detection into time detection to realize the detection of specific target molecules. The method can realize detection of different target molecules, is simple and convenient, does not need expensive instruments and professional operation, and can be applied to rapid detection of trace amount of hydroxyl polychlorinated biphenyl in a water sample.
Description
Technical Field
The invention relates to the technical field of biological detection, in particular to a target molecule detection method based on a molecularly imprinted polymer.
Background
Point of care test (POCT) is a detection technology that integrates rapidness, convenience, and intelligence, and is widely used in the fields of home healthcare, mobile medical care, field environment, battlefield medical care, and the like. At present, in field detection, a glucometer, a thermometer, a microfluidic chip and the like are mostly used for data reading, but the tools all need a conversion system. Therefore, the real-time inspection technology with simple principle, convenient operation, good selectivity and high accuracy has important practical requirements.
Molecularly Imprinted Polymers (MIPs) are polymers synthesized by molecular imprinting techniques that have specific recognition and selective adsorption of specific target molecules (template molecules) and structural analogs thereof. If the molecularly imprinted polymer is filled in the microchannel, permeability of the channel is reduced after adsorption with the target molecule, and if a certain amount of liquid is injected therein, time required for the liquid to completely flow out is increased. In the detection process aiming at target molecules, hydroxyl polychlorinated biphenyls (OH-PCBs) are common secondary pollutants and are generated by oxidizing the PCBs in a biological or non-biological mode, the detection method commonly used for the hydroxyl polychlorinated biphenyls in the prior art is an instrument method (GC-MS, LC-MS, MS and the like), the pretreatment process is complex and time-consuming, and the hydroxyl polychlorinated biphenyls in the water environment are difficult to effectively extract and detect.
Disclosure of Invention
The invention aims to provide a target molecule detection method based on a molecularly imprinted polymer, which can realize detection of different target molecules, is simple and convenient, does not need expensive instruments and professional operation, and can be applied to rapid detection of trace amounts of hydroxyl polychlorinated biphenyl in water samples.
The purpose of the invention is realized by the following technical scheme:
a method for detecting a target molecule based on a molecularly imprinted polymer, the method comprising:
and 4, recording the time when the water completely passes through the solid phase extraction column by using a timer, and converting the molecular detection into time detection to realize the detection of the specific target molecules.
According to the technical scheme provided by the invention, the method can realize detection of different target molecules, is simple and convenient, does not need expensive instruments and professional operation, and can be applied to rapid detection of trace amount of hydroxyl polychlorinated biphenyl in a water sample.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the description of the embodiments are briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on the drawings without creative efforts.
FIG. 1 is a schematic flow chart of a target molecule detection method based on a molecularly imprinted polymer according to an embodiment of the present invention;
FIG. 2 is a graphical representation of the relationship between the amount of acetonitrile used and Δ T in accordance with an embodiment of the present invention;
FIG. 3 is a graphical representation of pH vs. Δ T for an exemplary water sample in accordance with an embodiment of the present invention;
FIG. 4 is a graphical representation of the Δ T as a function of elution usage according to an embodiment of the present invention;
FIG. 5 is a graph showing the linear relationship between Δ T and the concentration of hydroxypolychlorobiphenyl in accordance with the embodiment of the present invention;
FIG. 6 is a schematic diagram of the selective detection of hydroxypolychlorobiphenyl according to an embodiment of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the present invention without making any creative effort, shall fall within the protection scope of the present invention.
The following will describe embodiments of the present invention in further detail with reference to the accompanying drawings, and as shown in fig. 1, a schematic flow chart of a target molecule detection method based on a molecularly imprinted polymer provided by the embodiments of the present invention is shown, where the method includes:
in the step, 50mg of the specific molecularly imprinted polymer can be weighed and filled in a polypropylene solid phase extraction column, wherein the column volume of the solid phase extraction column is 1.0 mL;
after filling, placing a polytetrafluoroethylene sieve plate on the upper part of the solid phase extraction column, and inserting a tool to compress tightly to prevent leakage.
In specific implementation, detection of different target molecules can be realized by setting the types of the molecularly imprinted polymers. For example, the molecularly imprinted polymer is set as the hydroxyl polychlorinated biphenyl molecularly imprinted polymer, so that the trace quantity of hydroxyl polychlorinated biphenyl in the water sample can be rapidly detected.
and 4, recording the time when the water completely passes through the solid phase extraction column by using a timer, and converting the molecular detection into time detection to realize the detection of the specific target molecules.
The detection principle of the method is as follows: the molecularly imprinted polymer can specifically identify and adsorb target molecules, so that the effective pipe diameter of the hourglass neck pipe of the solid-phase extraction column is reduced, and the time for quantitative water to flow out of the neck pipe is changed; and recording the time change by a timer, and converting the molecular detection into time detection to realize the detection of the specific target molecules.
In a specific implementation, the relationship between the concentration of a specific target molecule and the efflux time is specifically as follows:
ΔT＝2.02C+0.36(R2＝0.9979)
wherein, the delta T is the difference of the time required for 15ml of water to completely flow out when the target molecule exists or not; c is the concentration of a specific target molecule, which may be 4' -OH-PCB 30; and R is a fitting degree correlation coefficient, and the larger the value is, the more accurate the fitting degree of the formula is represented.
Based on the method, the rapid detection process of the trace amount of the hydroxyl polychlorinated biphenyl in the water sample specifically comprises the following steps:
firstly, activating a molecularly imprinted polymer solid phase extraction column by acetonitrile to enable the surface of the molecularly imprinted polymer and a target molecule to be easily adsorbed; wherein the molecularly imprinted polymer is a hydroxyl polychlorinated biphenyl molecularly imprinted polymer;
then 2ml deionized water is used for leaching the chromatographic column, and 50ml water sample liquid is added for sample loading;
leaching the solid phase extraction column with 2ml of deionized water to remove endogenous impurities and other impurities in the water sample;
and then taking 15mL of deionized water to flow through the solid phase extraction column, and recording the time when the water completely passes through the solid phase extraction column by using a timer, so as to realize the detection of the trace amount of the hydroxyl polychlorinated biphenyl in the water sample.
In a specific implementation, a diaphragm vacuum pump (0.088MPa) can be used to rapidly flow deionized water from the upper glass spheres through the neck of the solid phase extraction column.
In addition, the optimal amount of acetonitrile for activating the molecularly imprinted polymer solid phase extraction column is 2mL, and the reason for selecting the amount is specifically as follows:
respectively activating the molecularly imprinted polymer solid phase extraction column by acetonitrile with different volumes (0ml, 0.5ml, 1ml, 2ml, 3ml, 4ml, 5ml and 6ml) to enable the surface of the molecularly imprinted polymer and the target molecule to be easily adsorbed; eluting the chromatographic column with 2ml of deionized water, and adding 50ml of sample solution for sample loading; leaching the solid phase extraction column by using 2ml of deionized water to remove endogenous impurities and other impurities in the sample; finally, 15mL of deionized water was passed through the solid phase extraction column and the time for water to completely pass through the solid phase extraction column was recorded using a timer.
FIG. 2 is a graph showing the relationship between the amount of acetonitrile and Δ T, where Δ T gradually increases as the amount of acetonitrile increases from 0 to 2 mL; when the using amount of the acetonitrile is 2, the delta T is maximum; when the amount of acetonitrile used was increased from 3 to 6, Δ T gradually decreased. When the using amount of acetonitrile is less than 2mL, the wettability is insufficient, target molecules cannot be completely adsorbed, and the delta T is small; when the amount of acetonitrile is more than 2mL, the excess acetonitrile affects the adsorption of the target molecule, resulting in a decrease in Δ T. Therefore, when hydroxyl polychlorinated biphenyl is detected, the optimal using amount of acetonitrile for activating the molecularly imprinted polymer solid phase extraction column is 2 mL.
In addition, when hydroxyl polychlorinated biphenyl is detected, the optimal pH value of the water sample is 11, and the reason for selecting the dosage is specifically as follows:
the time signal is influenced because the pH value of the water sample can influence special bonds (including hydrogen bonds and ionic bonds) in molecules and hydrophobic interaction between target molecules and the molecularly imprinted polymer. Therefore, the pH value of the water sample is optimized, the pH value of the water sample is adjusted to 3.0-11.0 by using the sodium hydroxide solution, the influence of the pH value on the time signal is studied, as shown in fig. 3, a schematic diagram of the relationship between the pH value and the Δ T of the water sample is shown in the embodiment of the present invention, and when the pH is 3, the Δ T is large; at pH 11, Δ T is maximal and gradually decreases with increasing pH. The reasons for this phenomenon are: -NH of molecularly imprinted polymer under acidic conditions at pH 32Protonation is carried out, positive charge is carried, and cation-pi interaction (non-bond effect) is formed with the benzene ring of the hydroxyl polychlorinated biphenyl; and-NH at pH 5.0, 7.0 and 9.02The protonation gradually disappears, the non-bond effect is weakened, and the delta T is reduced; when the pH value is 11.0, the maximum delta T is formed by the fact that-OH on the hydroxyl polychlorinated biphenyl is ionized and carries negative charges, and charge-assisted N-H … O is formed between the hydroxyl polychlorinated biphenyl and the molecularly imprinted polymer(-)Hydrogen is stronger than ordinary hydrogen bonds. Therefore, when hydroxyl polychlorinated biphenyl is detected, the optimal pH value of the water sample is 11.
In addition, after detection of a specific target molecule, a 10ml volume ratio of 1: the n-hexane and acetonitrile solution of 1 is used for eluting the molecularly imprinted polymer adsorbing the specific target molecules, and the molecularly imprinted polymer can be repeatedly used after the elution is finished. FIG. 4 is a graph showing the change of Δ T with the number of elution times according to the example of the present invention, C(Hydroxypolychlorobiphenyl)80ppb, after 5 adsorbings and elutions, the Δ T has no significant change, indicating good reusability of the process of the invention.
Taking the detection of 4 '-OH-PCB 30 as an example, the detection was performed as described above for different concentrations (0, 20ppb, 40ppb, 60ppb, 80ppb) of 4' -OH-PCB30, and then the Δ T was recorded. As shown in fig. 5, which is a schematic diagram of the linear relationship between Δ T and the concentration of hydroxypolychlorobiphenyl according to the embodiment of the present invention, Δ T increases with the increase of the concentration of 4 '-OH-PCB 30 (15ml of water), and Δ T and the concentration of 4' -OH-PCB30 are in good linear relationship within the range of 1-100 ppb, the linear equation can be expressed as:
ΔT＝2.02C+0.36(R2＝0.9979)
wherein, the delta T is the difference of the time required for 15ml of water to completely flow out when the target molecule exists or not; c is the concentration of 4' -OH-PCB 30; r is a correlation coefficient;
the lowest detection concentration LOD is 0.3ppb (S/N is 3); LOD is the lowest concentration above which the target molecule is detected, or above which it is detected.
In addition, several different analytes such as 4-OH-4 '-iodobiphenyl, bisphenol A, PCB156, PCB101 and the like can be selected as the interferent, the concentration of the target molecule 4' -OH-PCB30 is 10ppb, and the concentrations of other interferent are all 1ppm, as shown in fig. 6, the method is a schematic diagram of the selective detection of hydroxypolychlorobiphenyl according to the embodiment of the present invention, when the interferent exists, Δ T is smaller, and the Δ T is significantly increased along with the increase of the concentration of the target molecule, which indicates that the method has high selectivity.
The following describes the process of the above detection method in detail by using a specific example, in this example, trace amounts of hydroxypolychlorobiphenyl in a water sample are detected, and the specific process is as follows:
collecting a certain amount of water sample from the pond, filtering impurities such as silt and the like by using a 0.45-micrometer filter membrane after filtering the impurities by using quantitative filter paper, and finally placing the water sample into a brown glass bottle for storage at 4 ℃; the pH of the water sample was adjusted to 11.0 with sodium hydroxide solution before testing.
Activating a molecularly imprinted polymer solid-phase extraction column by using 2ml of acetonitrile to enable the surface of the molecularly imprinted polymer and a target molecule to be easily adsorbed; eluting the chromatographic column with 2ml of deionized water, and adding 50ml of water sample liquid for sample loading; leaching the solid phase extraction column with 2ml of deionized water to remove endogenous impurities and other impurities in the water sample; and finally, taking 15mL of deionized water to flow through the solid phase extraction column, and recording the time for the water to completely pass through the molecularly imprinted polymer solid phase extraction column by adopting a handheld timer.
The method can be used for detecting the 4 '-OH-PCB 30 in the pond water sample, and the concentration of the 4' -OH-PCB30 in the pond water sample is 2.45 ppb. And 4' -OH-PCB30 with different concentrations (5ppb, 10ppb, 25ppb and 50ppb) is used for sample addition, the recovery rate is between 88.0% and 93.4%, and the RSD is less than 6.7%. The results show that: the method provided by the embodiment of the invention has a good application prospect in the detection of actual samples.
It is noted that those skilled in the art will recognize that embodiments of the present invention are not described in detail herein.
In conclusion, compared with the defect that a biological recognition system is greatly influenced by the environment, the detection method provided by the embodiment of the invention has the advantages that the molecularly imprinted polymer shows stronger stability in different environments, and has the characteristics of simple principle, convenience in operation, good selectivity, high accuracy and the like; if the variety of the molecularly imprinted polymer is changed, different target molecules can be detected, and the application range of the technology is greatly expanded.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are included in the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (9)
1. A target molecule detection method based on a molecularly imprinted polymer, which is characterized by comprising the following steps:
step 1, filling a solid phase extraction column with a specific molecularly imprinted polymer serving as an identification unit;
step 2, connecting the upper part of the solid phase extraction column with a container for containing water, and loading the lower part of the solid phase extraction column on a solid phase extraction instrument;
step 3, connecting the solid phase extraction column with a vacuum pump for vacuum pumping to enable water on the upper part to flow rapidly;
and 4, recording the time when the water completely passes through the solid phase extraction column by using a timer, and converting the molecular detection into time detection to realize the detection of the specific target molecules.
2. The method for detecting target molecules based on molecularly imprinted polymer according to claim 1, wherein in step 1, 50mg of the specific molecularly imprinted polymer is weighed and filled in a polypropylene solid phase extraction column, and the column volume of the solid phase extraction column is 1.0 mL;
after filling, placing a polytetrafluoroethylene sieve plate on the upper part of the solid phase extraction column, and inserting a tool to compress tightly to prevent leakage.
3. The method for detecting target molecules based on molecularly imprinted polymer according to claim 1, wherein in step 4, the relationship between the concentration of specific target molecules and the flowing-out time is expressed as:
ΔT＝2.02C+0.36(R2＝0.9979)
wherein, the delta T is the difference of the time required for 15ml of water to completely flow out when the target molecule exists or not; c is the concentration of a specific target molecule; and R is a fitting degree correlation coefficient, and the larger the value is, the more accurate the fitting degree of the formula is represented.
4. The method for detecting target molecules based on molecularly imprinted polymers of claim 1, wherein in step 1, the detection of different target molecules is achieved by setting the type of molecularly imprinted polymer.
5. The method for detecting a target molecule based on a molecularly imprinted polymer according to claim 4,
the molecular imprinting polymer is set as a hydroxyl polychlorinated biphenyl molecular imprinting polymer, so that the trace quantity of hydroxyl polychlorinated biphenyl in a water sample can be quickly detected.
6. The target molecule detection method based on the molecularly imprinted polymer according to claim 5, wherein the rapid detection process of trace amount of the hydroxypolychlorobiphenyl in the water sample specifically comprises the following steps:
firstly, activating a molecularly imprinted polymer solid phase extraction column by acetonitrile to enable the surface of the molecularly imprinted polymer and a target molecule to be easily adsorbed; wherein the molecularly imprinted polymer is a hydroxyl polychlorinated biphenyl molecularly imprinted polymer;
then 2ml deionized water is used for leaching the chromatographic column, and 50ml water sample liquid is added for sample loading;
leaching the solid phase extraction column with 2ml of deionized water to remove endogenous impurities and other impurities in the water sample;
and then taking 15mL of deionized water to flow through the solid phase extraction column, and recording the time when the water completely passes through the solid phase extraction column by using a timer, so as to realize the detection of the trace amount of the hydroxyl polychlorinated biphenyl in the water sample.
7. The method for detecting target molecules based on molecularly imprinted polymer according to claim 6, wherein the optimal amount of acetonitrile used for activating the molecularly imprinted polymer solid phase extraction column is 2 mL.
8. The method for detecting target molecules based on molecularly imprinted polymer as claimed in claim 6, wherein the optimal pH value of the water sample is 11 when hydroxyl polychlorinated biphenyl is detected.
9. The method for detecting a target molecule based on a molecularly imprinted polymer according to claim 1,
after detection of a specific target molecule, a 10ml volume ratio of 1: the n-hexane and acetonitrile solution of 1 is used for eluting the molecularly imprinted polymer adsorbing the specific target molecules, and the molecularly imprinted polymer can be repeatedly used after the elution is finished.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103776879A (en) * | 2014-02-28 | 2014-05-07 | 中国科学院烟台海岸带研究所 | Method for detecting organic pollutants through combination of molecular imprinting solid-phase extraction technology and potential method |
| CN104151489A (en) * | 2014-04-03 | 2014-11-19 | 中国科学院烟台海岸带研究所 | Hydrophilic hydroxyl polychlorinated biphenyl molecularly imprinted solid-phase extraction filler as well as preparation method and application thereof |
| CN110763539A (en) * | 2019-11-22 | 2020-02-07 | 福州大学 | Hydroxyl polychlorinated biphenyl detection method based on molecular imprinting column and weight system |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103776879A (en) * | 2014-02-28 | 2014-05-07 | 中国科学院烟台海岸带研究所 | Method for detecting organic pollutants through combination of molecular imprinting solid-phase extraction technology and potential method |
| CN104151489A (en) * | 2014-04-03 | 2014-11-19 | 中国科学院烟台海岸带研究所 | Hydrophilic hydroxyl polychlorinated biphenyl molecularly imprinted solid-phase extraction filler as well as preparation method and application thereof |
| CN110763539A (en) * | 2019-11-22 | 2020-02-07 | 福州大学 | Hydroxyl polychlorinated biphenyl detection method based on molecular imprinting column and weight system |
Non-Patent Citations (2)
| Title |
|---|
| RONGNING LIANG 等: "Determination of hydroxylated polychlorinated biphenyls by offline solid-phase extraction-liquidchromatography-tandem massspectro-metry using a molecularly imprinted polymer as a sorbent for sample preconcentration", 《TALANTA》 * |
| 赵焱 等: "环境水体中羟基多氯联苯离线和在线检测方法的构建", 《中国优秀博硕士论文全文数据库（硕士） 工程科技I辑》 * |
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