CN112980894A - Method for performing mixed fermentation on ethanol by using yam slag and bagasse - Google Patents
Method for performing mixed fermentation on ethanol by using yam slag and bagasse Download PDFInfo
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- CN112980894A CN112980894A CN202110241350.7A CN202110241350A CN112980894A CN 112980894 A CN112980894 A CN 112980894A CN 202110241350 A CN202110241350 A CN 202110241350A CN 112980894 A CN112980894 A CN 112980894A
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- bagasse
- ethanol
- fermentation
- dioscorea
- mixing
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
- C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P2201/00—Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Abstract
The invention discloses a method for performing mixed fermentation on ethanol by using dioscorea opposita residue and bagasse, and belongs to the technical field of biomass energy production. The method comprises the steps of taking dioscorea composita residues and bagasse as raw materials, carrying out alkaline pretreatment on the bagasse, carrying out pre-saccharification on the dioscorea composita residues, then mixing the pretreated bagasse and the pre-saccharified dioscorea composita residues, adding cellulase and yeast activation solution, and fermenting to obtain ethanol. The bagasse and the yam slag are selected as raw materials for co-fermentation, and the concentration of ethanol is increased along with the increase of solid load. By adding 0.1% (w/v) tween 80 in the batch feed box, the solids loading can be increased to 34% and 75-85g/L ethanol is produced without decreasing the yield of ethanol. The preparation process of the method has excellent stability. Meanwhile, the invention does not need to add extra materials and has low preparation cost.
Description
Technical Field
The invention relates to the technical field of biomass energy production, in particular to a method for performing mixed fermentation on ethanol by using yam slag-bagasse.
Background
With the excessive exploitation and utilization of fossil energy, the problems of increasing energy price and the like are increasingly prominent, and biomass energy is paid attention to due to the advantages of low production cost, cyclic utilization, few environmental problems and the like.
Dioscorea composita is an exotic species from Mexico, the starch content in tubers accounts for about 25% -28% of the fresh weight, and the rhizomes are mainly used for extracting diosgenin and producing fuel ethanol by fermentation.
The direct use of dioscorea composita as a starch raw material for producing ethanol by fermentation is difficult, so that the ethanol produced by dioscorea composita starch fermentation is utilized as a byproduct on the basis of utilization of dioscorea composita steroid saponin resources, and specifically comprises two types: one is sugar solution pretreated in the production process of dioscorea composita hydrolyzed saponin, and the other is residue after dioscorea composita saponin extraction. However, the ethanol produced by directly fermenting the two types of yam starch has low concentration, so that the production application is limited.
Disclosure of Invention
The invention aims to provide a method for fermenting ethanol by mixing yam slag and bagasse, which aims to solve the problems.
According to one aspect of the invention, the method for fermenting the ethanol by mixing the dioscorea composita residues and the bagasse is used as raw materials, the bagasse is subjected to alkaline pretreatment, the dioscorea composita residues are subjected to pre-saccharification, the pretreated bagasse and the pre-saccharified dioscorea composita residues are mixed, and cellulase and yeast activation solution are added for fermentation to obtain the ethanol.
In some embodiments, the bagasse is subjected to an alkaline pretreatment step as follows: bagasse was immersed in an alkaline solution, then washed to neutrality, dried and sterilized.
Wherein the alkaline solution is 0.1-1mol/L sodium hydroxide solution, the soaking temperature is 70-80 ℃, the soaking time is 1-3h, and the mass volume ratio of the bagasse to the sodium hydroxide solution is 1: 20-30.
The plastid ratio referred to in the invention is the ratio of the mass of the bagasse to the volume of the sodium hydroxide solution, and the unit is g/mL or kg/L. Preferably, the soaking is also carried out with stirring at a rate of 100-150rpm, thereby allowing faster penetration of the alkaline solution into the bagasse interior.
In some embodiments, the pre-saccharification step is performed on the dioscorea composita residue as follows: preheating Dioscorea composita residue at 80-90 deg.C, adding acid to adjust pH to 5-5.6, adding liquefying enzyme to liquefy, cooling to 60-70 deg.C, adding acid to adjust pH to 4-5, adding saccharifying enzyme to saccharify to obtain pre-saccharified Dioscorea composita residue.
Wherein the addition amount of the liquefying enzyme is added according to the loading amount of 100-;
adding the saccharifying enzyme according to the loading amount of 10-30U per gram of pretreated dioscorea composita residue, and saccharifying for 1-3 h;
preferably, the liquefaction and saccharification are also carried out with simultaneous stirring, the stirring speed being 100-150 rpm. Thereby allowing the reaction to proceed more quickly.
In some embodiments, the pre-treated bagasse and pre-saccharified dioscorea composita residue are mixed in a mass ratio of 1: 1-5.
In some embodiments, cellulase is added at a loading of 10-20FPU per gram of pretreated bagasse.
In some embodiments, the yeast activation solution is added in an amount of 3-6mL, wherein the initial yeast cells are contained at a mass concentration of 3-4 g/L.
In some embodiments, the temperature of the mixed fermentation is 30-37 ℃, and the fermentation is performed in a constant temperature shaking table at the rotation speed of 100-150 rpm. Thereby allowing the reaction to proceed more quickly.
In some embodiments, the method for fermentation of ethanol by dioscorea opposita-bagasse mixing comprises the following steps:
(1) alkali pretreatment of bagasse: putting bagasse into an alkaline solution for soaking, then taking out and washing to be neutral, and drying to obtain pretreated bagasse;
(2) pretreating the dioscorea composita residue: drying the dioscorea composita residue to obtain pretreated dioscorea composita residue;
(3) pre-saccharifying dioscorea composita residues: preheating the pretreated dioscorea composita residues at 80-90 ℃, adding acid to adjust pH to 5-6, adding liquefying enzyme to liquefy, cooling to 60-70 ℃, adjusting pH to 4-5, adding saccharifying enzyme to saccharify to obtain pre-saccharified dioscorea composita residues;
(4) mixing and fermenting: mixing pretreated bagasse and pre-saccharified Dioscorea composita residue, adding water, adjusting pH to 4-5, adding cellulase and yeast activation solution, adding sterile water to the system, adding 0.1% (w/v) Tween 80, mixing, saccharifying, and fermenting to obtain ethanol.
The invention selects bagasse and dioscorea composita residue as co-fermentation raw materials, and the concentration of ethanol increases along with the increase of solid load. By adding 0.1% (w/v) tween 80 in the batch feed box, the solids loading can be increased to 34% and 75-85g/L ethanol is produced without decreasing the yield of ethanol. The preparation process of the method has excellent stability. Meanwhile, the invention does not need to add extra materials and has low preparation cost.
Detailed Description
The present invention will be described in further detail with reference to examples.
Example 1
The method for fermenting ethanol by mixing the yam slag and the bagasse comprises the following steps:
(1) alkali pretreatment of bagasse: the alkaline treatment is carried out in a 1.0L reactor, 10g of bagasse is put into 200mL of 0.5mol/L sodium hydroxide solution to be soaked for 2h at 80 ℃, the mixture is stirred at 400rpm to obtain bagasse residues, then the bagasse residues are taken out and washed to be neutral by water, the neutral bagasse residues are placed in a 50 ℃ oven to be dried for 24h, and then the bagasse residues are sterilized for 20min at 121 ℃ to obtain pretreated bagasse;
(2) pretreating the dioscorea composita residue: sieving Dioscorea composita residue with 40 mesh sieve, and oven drying at 60 deg.C for 12 hr to obtain pretreated Dioscorea composita residue;
(3) pre-saccharifying dioscorea composita residues: preheating the pretreated dioscorea composita residues at 85 ℃, adding hydrochloric acid to adjust the pH value to about 5.5, adding liquefying enzyme to liquefy, adding the liquefying enzyme according to the load of 150U per gram of the pretreated dioscorea composita residues, liquefying for 2 hours, synchronously stirring at the stirring speed of 120rpm, cooling to 60 ℃, adjusting the pH value to 4.5 with hydrochloric acid, adding saccharifying enzyme to saccharify, adding the saccharifying enzyme according to the load of 20U per gram of the pretreated dioscorea composita residues, saccharifying for 2 hours, synchronously stirring at the stirring speed of 120rpm, and obtaining the prehydrolyzed dioscorea composita residues;
(4) mixing and fermenting: mixing pretreated bagasse and pre-saccharified dioscorea composita residues according to a mass ratio of 1:3, adding water, adjusting the pH to about 4.5 by using 1.0mol/L sulfuric acid, adding cellulase according to a load of 15FPU per gram of pretreated bagasse, adding 5mL of yeast activation solution, wherein the mass concentration of initial yeast cells in the yeast activation solution is 3.3g/L, then supplementing sterile water to a system to reach 100g, adding 0.1% (w/v) of Tween 80 into a batch feeding box, performing mixed fermentation at a fermentation temperature of 34 ℃, performing synchronous stirring at a stirring speed of 120rpm, and fermenting to obtain ethanol.
The detection proves that the yield of the ethanol obtained by the embodiment reaches 82 g/L.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Claims (10)
1. A method for fermentation of ethanol by mixing yam bagasse and bagasse is characterized in that the bagasse is subjected to alkaline pretreatment, the dioscorea composita residue is subjected to pre-saccharification, the pretreated bagasse and the pre-saccharified dioscorea composita residue are mixed, and cellulase and yeast activation liquid are added for fermentation to obtain the ethanol.
2. The method for fermentation of ethanol by mixing of yam bagasse and bagasse according to claim 1, wherein the bagasse is subjected to an alkaline pretreatment step as follows: bagasse was immersed in an alkaline solution, then washed to neutrality, dried and sterilized.
3. The method for performing mixed fermentation on ethanol by using the yam slag-bagasse according to claim 2, wherein the alkaline solution is 0.1-1mol/L sodium hydroxide solution, the soaking temperature is 70-80 ℃, the soaking time is 1-3h, and the mass-to-volume ratio of the bagasse to the sodium hydroxide solution is 1: 20-30.
4. The method for fermentation of ethanol by mixing dioscorea opposita pomace and bagasse according to claim 1, wherein the pre-saccharification step of dioscorea composita pomace comprises the following steps: preheating Dioscorea composita residue at 80-90 deg.C, adding acid to adjust pH to 5-5.6, adding liquefying enzyme to liquefy, cooling to 60-70 deg.C, adding acid to adjust pH to 4-5, adding saccharifying enzyme to saccharify to obtain pre-saccharified Dioscorea composita residue.
5. The method for fermentation of ethanol by mixing dioscorea zingiberensis rhizome residue and bagasse as claimed in claim 4, wherein the amount of the liquefying enzyme is 100-.
6. The method for performing mixed fermentation on ethanol by using the dioscorea zingiberensis rhizome residue and the bagasse according to claim 4, wherein the saccharifying enzyme is added according to the proportion of 10-30U per gram of pretreated dioscorea composita rhizome residue, and the saccharifying time is 1-3 h.
7. The method for fermentation of ethanol by mixing of yam slag and bagasse according to any one of claims 1 to 6, wherein the pre-treated bagasse and the pre-saccharified dioscorea composita slag are mixed in a mass ratio of 1:1 to 5.
8. The method for fermentation of ethanol by mixing yam bagasse and bagasse according to claim 7, wherein the amount of cellulase added is 10-20FPU per gram of pretreated bagasse.
9. The method for performing mixed fermentation on ethanol by using the dioscorea opposita-bagasse according to claim 7, wherein the yeast activation solution is added in an amount of 3-6mL, and the mass concentration of the primary yeast cells in the yeast activation solution is 3-4 g/L.
10. The method for fermentation of ethanol by mixing yam rhizome residue and bagasse as claimed in claim 8 or 9, wherein the temperature of the mixing fermentation is 30-37 ℃, the fermentation is performed in a shaker at a rotation speed of 100-130 rpm.
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Citations (5)
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| CN102061319A (en) * | 2010-11-09 | 2011-05-18 | 佛山市正合生物能源有限公司 | Method for preparing biological fatty oil from Dioscorea camposita |
| US20130337526A1 (en) * | 2011-02-24 | 2013-12-19 | Tsukishima Kikai Co., Ltd. | Method of Producing Ethanol |
| CN104321435A (en) * | 2012-03-30 | 2015-01-28 | Ctc-蔗糖技术中心不记名股份公司 | System and method for the integrated production of first- and second-generation ethanol and the use of integration points for said production |
| CN108220347A (en) * | 2011-12-22 | 2018-06-29 | 希乐克公司 | Production of sugars and alcohols from biomass |
| CN110564780A (en) * | 2019-09-16 | 2019-12-13 | 华南农业大学 | Method for co-producing biogas by fermenting ethanol with bagasse-molasses-yam slag mixed raw material |
-
2021
- 2021-03-04 CN CN202110241350.7A patent/CN112980894A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102061319A (en) * | 2010-11-09 | 2011-05-18 | 佛山市正合生物能源有限公司 | Method for preparing biological fatty oil from Dioscorea camposita |
| US20130337526A1 (en) * | 2011-02-24 | 2013-12-19 | Tsukishima Kikai Co., Ltd. | Method of Producing Ethanol |
| CN108220347A (en) * | 2011-12-22 | 2018-06-29 | 希乐克公司 | Production of sugars and alcohols from biomass |
| CN104321435A (en) * | 2012-03-30 | 2015-01-28 | Ctc-蔗糖技术中心不记名股份公司 | System and method for the integrated production of first- and second-generation ethanol and the use of integration points for said production |
| CN110564780A (en) * | 2019-09-16 | 2019-12-13 | 华南农业大学 | Method for co-producing biogas by fermenting ethanol with bagasse-molasses-yam slag mixed raw material |
Non-Patent Citations (2)
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| GUANGYING YE ET AL: "Ethanol production from mixtures of sugarcane bagasse and Dioscorea composita extracted residue with high solid loading", 《BIORESOURCE TECHNOLOGY》 * |
| GUANGYING YE ET AL: "Ethanol production from mixtures of sugarcane bagasse and Dioscorea composita extracted residue with high solid loading", 《BIORESOURCE TECHNOLOGY》, vol. 257, 7 February 2018 (2018-02-07), pages 24 - 25 * |
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Application publication date: 20210618 |