CN112972438B - Lignan compound from radix paeoniae rubra, and preparation method and application thereof - Google Patents
Lignan compound from radix paeoniae rubra, and preparation method and application thereof Download PDFInfo
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Abstract
The invention belongs to the field of medicines, and relates to lignan compounds in red paeony root, a preparation method thereof and application of the compounds in preventing and treating allergic diseases. Pharmacological experiments prove that the compound can effectively inhibit the release of beta-hexosaminidase (beta-HEX) and histamine (His) in sensitized RBL-2H3 cells, can obviously reduce the content of inflammatory mediators in the supernatant of RBL-2H3 cells, has good antiallergic, antiallergic and anti-inflammatory effects, and can reach an effective dose of 5 mu mol/L. Can be used for preparing medicines for preventing and treating allergic diseases.
Description
Technical Field
The invention belongs to the field of medicines, and in particular relates to a compound separated from Chinese medicinal red paeony root and application thereof in preventing and treating allergic diseases.
Background
Allergic diseases are chronic diseases, which seriously affect the working efficiency of patients, and reduce the production capacity of the patients, thereby leading to the improvement of social cost. Thus, effective and safe treatment of allergic diseases is one of the major challenges facing public health. Allergic diseases include allergic reactions (allergic reaction) and anaphylactoid reactions (anaphylactoid reaction). The allergic reaction is the most urgent and potential serious manifestation of allergic diseases, and refers to a physiological change mainly caused by physiological dysfunction or tissue cell injury of an organism when the organism receives the same antigen stimulus again after the organism responds to an antigen substance for the first time. According to the report of the world allergic reaction organization, people with allergic symptoms worldwide account for 30% -40% of the general population; the number of patients is large, and the data are often underestimated because symptoms seriously damaging life health do not appear in early and middle stages of anaphylaxis. Most of the antiallergic drugs currently on the market have serious adverse effects such as arrhythmia, mental dysfunction, gastrointestinal disorder and infection. It is therefore imperative to search for new and effective therapeutic regimens.
Type I hypersensitivity and allergy-like reactions are similar in clinical symptoms, but the difference between the action mechanisms of the two is not completely understood at present. However, a great deal of research shows that both anaphylactic reaction and anaphylactic reaction can cause the body to release beta-hexosaminidase and histamine, cause smooth muscle contraction, increase vascular permeability and the like, thereby causing a series of anaphylactic symptoms. The RBL-2H3 cell cytoplasm contains abundant basophilic particles, and can cause degranulation phenomenon when the cell generates type I anaphylactic reaction or anaphylactic reaction, namely, the alkaline particles wrapping histamine and beta-hexosaminidase gradually approach to the cell membrane, the vesicle membrane is fused with the cell membrane, and the content is released outside the cell to induce the generation of corresponding anaphylactic symptoms. In view of this, many scholars consider: inhibiting the release of allergic mediators can be a viable direction for the development of new antiallergic and antiallergic agents.
The type I hypersensitivity and the anaphylactic reaction are difficult to distinguish through symptoms in clinical diagnosis, and the main pathological changes of the type I hypersensitivity and the anaphylactic reaction are smooth muscle contraction, telangiectasia, increased vascular permeability and increased gland secretion, so that the elimination and alleviation of the symptoms become necessary links for resisting allergic diseases. The above-mentioned series of symptoms are caused by a series of inflammatory mediators released by the body, wherein the mediators mainly play a role include His and beta-HEX, and thus, inhibition of the release of the two mediators becomes a key for the treatment of allergic diseases. The natural product has the advantages of small toxic and side effects, lasting curative effect, good overall regulation and synergistic effect and the like, and becomes a research hot spot of new medicines for treating allergic diseases in recent years. Although the efficacy evaluation and mechanism discussion of the natural products are still based on laboratory research stages, clinical research has not provided definitive conclusions, a great amount of data has laid a certain theoretical foundation, and a foundation is provided for further optimizing new anti-allergic disease drugs.
Radix Paeoniae Rubra (Paeoniae Rubra Radix) is dried root of radix Paeoniae Rubra (Paeonia lactiflora Pall) of Ranunculaceae. Enter liver meridian. The Chinese medicine is usually combined for treating symptoms such as toxic heat, spot, conjunctival congestion, swelling and pain, liver Yu Xie pain, amenorrhea, dysmenorrhea, traumatic injury, carbuncle, swelling and sore. Modern pharmacology finds that the radix paeoniae rubra has the effects of resisting bacteria, resisting inflammation and relieving pain. The red paeony root has complex chemical components, mainly monoterpenes, fatty acids, phenols, lignans and other compounds.
The applicant researches find that the lignan compounds I and II are separated from red paeony root and have the following chemical structures, pharmacological experiments prove that the compounds can effectively reduce the release amount of RBL-2H3 cell histamine and beta-hexosaminidase induced by allergen, have good effect of inhibiting cell degranulation, thus showing good potential of resisting allergic diseases, and the effective dose can reach 5 mu mol/L. At present, research reports on the application of the radix paeoniae rubra extract in treating allergic diseases are not seen, and patent documents of a preparation method of the radix paeoniae rubra compound monomer for inhibiting RBL-2H3 degranulation are not seen.
Disclosure of Invention
The invention aims to solve the technical problem of providing a novel medicine for resisting allergic diseases.
The first aspect of the present invention provides a class of compounds derived from red peony root;
the second aspect of the present invention provides a process for the preparation of such compounds;
in a third aspect the present invention provides pharmaceutical compositions comprising such compounds;
in a fourth aspect, the invention provides the use of such compounds in the treatment of allergic diseases.
In order to solve the technical problems, the following technical scheme is adopted:
the first aspect of the invention provides a compound I, II, III and IV derived from red paeony root, which has the following structure:
the second aspect of the present invention provides a method for preparing the above red peony compound, comprising the steps of: the red paeony root medicinal materials are soaked in distilled water, then are subjected to ultrasonic extraction, concentrated extract is subjected to organic solvent extraction, macroporous adsorption resin chromatography, gel column chromatography, reversed phase silica gel column chromatography and preparative HPLC separation and purification, and the obtained compounds I, II, III and IV are analyzed and identified by the spectroscopic means such as UV, IR, NMR, MS, CD and the like to identify the structures of the compounds I, II, III and IV, and the compounds I, II, III are lignan compounds.
The third aspect of the present invention is directed to a pharmaceutical composition comprising a pharmaceutically effective amount of a compound and a pharmaceutically acceptable carrier. Typically, the pharmaceutical compositions of the present invention contain 0.1% to 96% by weight of the compound of the present invention. The compounds of the invention are generally present in unit dosage forms in amounts of from 0.1 to 100mg, with preferred unit dosage forms containing from 5 to 60mg.
Pharmaceutical compositions of the compounds of the present invention may be prepared according to methods well known in the art. For this purpose, the compounds of the invention may, if desired, be combined with one or more solid or liquid pharmaceutical excipients and/or auxiliaries, in suitable administration forms or dosage forms which can be used as human or veterinary medicine.
The compounds of the present invention or pharmaceutical compositions containing them may be administered in unit dosage form by the enteral or parenteral route, such as oral, intramuscular, subcutaneous, nasal, oral mucosal, dermal, ocular, peritoneal or rectal, etc.
The route of administration of the compounds of the invention or pharmaceutical compositions containing them may be by injection. The injection includes intravenous injection, subcutaneous injection, intradermal injection, acupoint injection, etc.
The administration dosage form may be liquid dosage form or solid dosage form. For example, the liquid dosage form may be true solution, colloid, microparticle, emulsion, or suspension. Other dosage forms such as tablet, capsule, dripping pill, aerosol, pill, powder, solution, suspension, emulsion, granule, suppository, eye drop, lyophilized powder for injection, etc.
The compound of the invention can be prepared into common preparations, sustained release preparations, controlled release preparations, targeted preparations and various microparticle administration systems.
For example, in order to prepare a unit dosage form into a tablet, various carriers known in the art can be widely used. Examples of carriers are, for example, diluents and absorbents such as starch, dextrin, calcium sulfate, lactose, mannitol, sucrose, sodium chloride, glucose, urea, calcium carbonate, kaolin, microcrystalline cellulose, aluminum silicate, etc.; humectants and binders such as water, glycerin, polyethylene glycol, ethanol, propanol, starch slurry, dextrin, syrup, honey, dextrose solution, gum arabic slurry, gelatin slurry, sodium carboxymethyl cellulose, shellac, methyl cellulose, potassium phosphate, polyvinylpyrrolidone, and the like; disintegrants such as dry starch, alginate, agar powder, brown algae starch, sodium bicarbonate and citric acid, calcium carbonate, polyoxyethylene sorbitol fatty acid ester, sodium dodecyl sulfonate, methylcellulose, ethylcellulose, etc.; disintegration inhibitors such as sucrose, glyceryl tristearate, cocoa butter, hydrogenated oils and the like; absorption promoters such as quaternary ammonium salts, sodium lauryl sulfate, and the like; lubricants such as talc, silica, corn starch, stearate, stearic acid, liquid paraffin, polyethylene glycol and the like. The tablets may be further formulated into coated tablets, such as sugar coated tablets, film coated tablets, enteric coated tablets, or bilayer and multilayer tablets.
For example, in order to make the administration unit into a pill, various carriers well known in the art can be widely used. Examples of carriers are, for example, diluents and absorbents such as glucose, lactose, starch, cocoa butter, hydrogenated vegetable oils, polyvinylpyrrolidone, glycerol monostearate, kaolin, talc and the like; binders such as acacia, tragacanth, gelatin, ethanol, honey, liquid sugar, rice paste or batter, and the like; disintegrants such as agar powder, dry starch, alginate, sodium dodecyl sulfonate, methylcellulose, ethylcellulose, etc.
For example, in order to make the administration unit into a capsule, the active ingredient of the compound of the present invention is mixed with the above-mentioned various carriers, and the thus-obtained mixture is placed in a hard gelatin capsule or a soft capsule. The active ingredient of the compound can be prepared into microcapsules, and the microcapsules can be suspended in an aqueous medium to form a suspension, or can be filled into hard capsules or prepared into injection for application.
For example, the compounds of the present invention may be formulated as injectable formulations, such as solutions, suspensions, emulsions, freeze-dried powder injection solutions, which may be aqueous or non-aqueous, and may contain one or more pharmaceutically acceptable carriers, diluents, binders, lubricants, preservatives, surfactants or dispersants. For example, the diluent may be selected from water, ethanol, polyethylene glycol, 1, 3-propanediol, ethoxylated isostearyl alcohol, polyoxy isostearyl alcohol, polyoxyethylene sorbitol fatty acid ester. In addition, in order to prepare an isotonic injection, an appropriate amount of sodium chloride, glucose or glycerin may be added to the preparation for injection, and further, a conventional cosolvent, a buffer, a pH adjuster, and the like may be added. These adjuvants are commonly used in the art.
In addition, colorants, preservatives, flavors, flavoring agents, sweeteners, or other materials may also be added to the pharmaceutical formulation, if desired.
For the purpose of administration, the drug or the pharmaceutical composition of the present invention can be administered by any known administration method to enhance the therapeutic effect.
The dosage of the pharmaceutical composition of the present invention to be administered depends on many factors such as the nature and severity of the disease to be prevented or treated, the sex, age, weight, character and individual response of the patient or animal, the route of administration, the number of times of administration, the purpose of treatment, and thus the therapeutic dosage of the present invention may vary widely. Generally, the dosages of pharmaceutical ingredients used in the present invention are well known to those skilled in the art. The amount of the actual drug contained in the final formulation of the compound composition of the present invention may be appropriately adjusted to achieve the therapeutically effective amount thereof, thereby achieving the preventive or therapeutic object of the present invention. Daily suitable dosage range of the compounds of the invention: the amount of the compound of the present invention is 0.001 to 100mg/Kg body weight, preferably 0.01 to 75mg/Kg body weight, more preferably 0.05 to 50mg/Kg body weight, most preferably 0.06 to 10mg/Kg body weight. The compound of the invention is taken by adult patients at a daily dose of 1-300 mg, preferably 4-150 mg, and can be taken once or 2-3 times; the dosage of the children is 0.01-15 mg, preferably 0.06-5 mg/kg body weight. The above-mentioned dosages may be administered in a single dosage form or in divided dosage forms, for example, two, three or four dosage forms, which are limited by the clinical experience of the administering physician and the administration regimen of the therapeutic means. The compounds or compositions of the present invention may be administered alone or in combination with other therapeutic or symptomatic agents.
A fourth aspect of the invention relates to the use of the above-mentioned red peony compound against allergic diseases. The results of in vitro antiallergic and antiallergic pharmacological tests show that the compound can effectively inhibit the release of RBL-2H3 cell histamine and beta-hexosaminidase, and when the administration concentration reaches 50 mu M, the survival rates of RBL-2H3 cells are 87.31%, 99.66%, 80.33% and 85.45% respectively. For allergic reactions, the compound may change the histamine release rate from 55.51% to 30.88%, 35.64%, 37.41%, 35.64%; the compound can change the release rate of beta-hexosaminidase from 43.73% to 26.42%, 33.81%, 33.64%, 30.56%. For anaphylactoid reactions, the compound can reduce histamine release rate from 49.23% to 25.49%, 30.92%, 24.21%, 32.39%, respectively; the compound can reduce the release rate of beta-hexosaminidase from 12.73% to 6.38%, 8.32%, 6.34%, 8.25%. Therefore, the medicine can achieve the aim of well inhibiting type I hypersensitivity and anaphylactoid reaction by selectively inhibiting the release of histamine and beta-hexosaminidase.
The beneficial technical effects are as follows:
1. the red paeony root compounds have obvious antiallergic and antiallergic effects, and firstly, can obviously inhibit the release of histamine, and the drug effect can reach 5mol/L. Secondly, the release of the-hexosaminidase can be obviously inhibited, and the drug effect reaches 5mol/L.
2. The red paeony root compound has the potential of further developing medicines for preventing and treating allergy and allergy-like diseases.
Drawings
FIG. 1, preparation flow of radix Paeoniae Rubra parts CS-1, CS-2, CS-3, CS-4.
Detailed Description
The following examples and pharmacological activity experiments are provided to further illustrate the invention, but are not meant to limit the invention in any way.
1. Preparation method of red paeony root monomer compounds I, II, III and IV
Example 1 preparation of Compounds I, II, III, IV
50kg of red paeony root decoction pieces, soaking in distilled water, and then carrying out ultrasonic extraction for 3 times, each time for 1 hour. Separating the water extract directly with macroporous adsorbent resin, eluting with 50% ethanol, and concentrating the eluate under reduced pressure. Separating the 50% ethanol eluate by MCI column, sequentially eluting with 30% ethanol and 50% ethanol, and concentrating the eluate under reduced pressure, wherein the 30% ethanol eluate is CS-3 (2000 g), and the 50% ethanol eluate is CS-4 (316 g). The residue was concentrated to no alcohol by 95% ethanol extraction, extracted 5 times with equal volume of ethyl acetate, and the extracts concentrated under reduced pressure to give ethyl acetate fraction CS-1 (580 g) and aqueous fraction CS-2 (270 g), as shown in FIG. 1.
We have found that only CS-4 in the above red peony sites has antiallergic effect by prior activity screening. Thus, the red peony site CS-4 was further extracted: dissolving radix Paeoniae Rubra CS-4 in methanol, and further extracting radix Paeoniae Rubra: dissolving the CS-4 methanol at the red peony root part, separating by a SephadexLH-20 column, and eluting with 50% methanol and methanol respectively to obtain components CS-4-1-CS-4-8. CS-4-4 (12.9 g) was isolated by reverse phase medium pressure chromatography, conditions: 20% methanol-water (40 min), 20% -100% methanol-water (230 min) and 100% methanol (60 min) to obtain components A1 and A2, wherein component A2 (8.3 g) is subjected to silica gel column chromatography (dichloromethane-methanol 50:1-10:1), and components B1-B9 are separated. B3 is separated by silica gel column chromatography (petroleum ether-acetone 10:1-2:1) to obtain D1-D11, D7 is separated by a preparation thin layer chromatography plate (methylene dichloride-acetone-methanol 15:1:1) to obtain D7-1-D7-4, and D7-3 is separated by a preparation liquid chromatography (RpC, 31% acetonitrile-water, 230 nm) to obtain D7-3-1-D7-3; d7-3-2 was then separated by preparative liquid chromatography (RpC, 20% acetonitrile-water, 230 nm) to give compound III (8 mg); d7-3-3 was further separated by preparative liquid chromatography (RpC, 22% acetonitrile-water, 230 nm) to give compound I (11 mg). A1 is separated by SephadexLH-20 (dichloromethane-methanol 1:1) to obtain A1-1 and A1-2; separating A1-1 by silica gel column chromatography (dichloromethane-methanol 100:1-1:1) to obtain H1-H7; h5-1 to H5-4 are obtained by separating H5 by a preparative thin layer chromatography plate (dichloromethane-methanol 8:1), and compound II (9 mg) is obtained by separating H5-1 by preparative liquid chromatography (RpC, 14% acetonitrile-water, 230 nm); h6-1 to H6-4 were obtained by separating H6 by a preparative thin layer chromatography plate (dichloromethane-methanol 15:1), and Compound IV (5 mg) was obtained by separating H6-2 by preparative liquid chromatography (RpC, 20% acetonitrile-water, 230 nm). The structure of the compound is analyzed and identified by UV, IR, NMR, MS and other spectroscopic means, and the compound is lignan compound.
The above compound spectrum information is as follows:
i: white powderAnd (3) powder; ESI-MS m/z 385[ M+Na ]] + ,401[M+K] + ,361[M-H] - ,397[M+Cl] - ； 1 H NMR(CD3OD,600MHz)δ:6.60(2H,d,J＝1.9Hz,H-2,2′),6.67(2H,d,J＝7.9Hz,H-5,5′),6.56(2H,dd,J＝7.9,1.9Hz,H-6,6′),2.57(2H,dd,J＝13.8,7.8Hz,H-7a,7′a),2.67(2H,dd,J＝13.8,6.9Hz,H-7b,7′b),1.92(2H,m,H-8,8′),3.60(4H,qd,J＝11.1,5.0Hz,H-9,9′),3.75(6H,s,3,3′-OMe)； 13 C NMR(CD 3 OD,150MHz)δ:134.0(C-1,1′),113.5(C-2,2′),149.0(C-3,3′),145.6(C-4,4′),115.9(C-5,5′),122.9(C-6,6′),36.2(C-7,7′),44.3(C-8,8′),62.3(C-9,9′),56.3(3,3′-OMe)。
II: a pale yellow powder; [ alpha ]] 20 D +44.7(c 0.15,MeOH)；ESI-MS m/z 399[M+Na] + ,411[M+Cl] - ； 1 H NMR(CD 3 OD,500MHz)δ:7.00(2H,d,J＝1.9Hz,H-2,2′),6.75(2H,d,J＝8.1Hz,H-5,5′),6.84(2H,dd,J＝8.1,1.9Hz,H-6,6′),4.90(2H,d,J＝8.5Hz,H-7,7′),2.28(2H,ddd,J＝8.5,5.0,3.1Hz,H-8,8′),3.66(2H,dd,J＝11.4,3.1Hz,H-9a,9′a),3.57(2H,dd,J＝11.4,5.0Hz,H-9b,9′b),3.85(6H,s,3,3′-OMe)； 13 C NMR(CD 3 OD,125MHz)δ:134.9(C-1,1′),111.2(C-2,2′),149.1(C-3,3′),147.3(C-4,4′),116.0(C-5,5′),120.5(C-6,6′),84.4(C-7,7′),55.3(C-8,8′),61.7(C-9,9′),56.4(3,3′-OMe)。
III: a pale brown oily liquid; ESI-MS m/z 383[ M+Na ]] + ,399[M+K] + ,395[M+Cl] - ； 1 H NMR(CD3OD,500MHz)δ:6.91(1H,d,J＝1.9Hz,H-2),6.73(1H,d,J＝8.1Hz,H-5),6.79(1H,dd,J＝8.1,1.9Hz,H-6),5.46(1H,d,J＝6.2Hz,H-7),3.43(1H,m,H-8),3.72(1H,dd,J＝11.0,7.2Hz,H-9a),3.80(1H,dd,J＝11.0,5.5Hz,H-9b),6.69(1H,s,H-2′),6.69(1H,s,H-6′),2.59(2H,t,J＝6.6Hz,H-7′),1.78(2H,dt,J＝6.6,6.6Hz,H-8′),3.53(2H,t,J＝6.6Hz,H-9′),3.78(3H,s,3-OMe),3.82(3H,s,3′-OMe)； 13 C NMR(CD 3 OD,125MHz)δ:136.9(C-1),114.0(C-2),147.5(C-3),147.4(C-4),116.1(C-5),117.9(C-6),89.0(C-7),55.5(C-8),64.9(C-9),129.8(C-1′),110.4(C-2′),145.2(C-3′),149.0(C-4′),134.8(C-5′),119.7(C-6′),35.8(C-7′),32.9(C-8′),62.2(C-9′),56.3(3-OMe),56.6(3′-OMe)。
IV: white powder; ESI-MS m/z 401[ M+Na ]] + ,417[M+K] + ,413[M+Cl] - ； 1 H NMR(CD 3 OD,500MHz)δ:6.99(1H,d,J＝1.7Hz,H-2),6.94(1H,d,J＝8.2Hz,H-5),6.82(1H,dd,J＝8.2,1.7Hz,H-6),4.84(1H,d,J＝6.0Hz,H-7),4.16(1H,ddd,J＝6.0,5.2,4.0Hz,H-8),3.41(1H,dd,J＝11.9,5.2Hz,H-9a),3.67(1H,dd,J＝11.9,4.0Hz,H-9b),6.82(1H,d,J＝2.0Hz,H-2′),6.72(1H,d,J＝8.1Hz,H-5′),6.68(1H,dd,J＝8.1,2.0Hz,H-6′),2.59(2H,t,J＝7.5Hz,H-7′),1.78(2H,m,H-8′),3.52(2H,t,J＝6.5Hz,H-9′),3.79(3H,s,3-OMe),3.82(3H,s,3′-OMe)； 13 C NMR(CD 3 OD,125MHz)δ:138.3(C-1),111.8(C-2),149.0(C-3),147.3(C-4),116.0(C-5),120.9(C-6),74.3(C-7),87.9(C-8),62.0(C-9),133.9(C-1′),114.0(C-2′),151.8(C-3′),147.7(C-4′),119.7(C-5′),122.2(C-6′),32.9(C-7′),35.7(C-8′),62.3(C-9′),56.4(3-OMe),56.6(3′-OMe)。
2. Pharmacological Activity experiment of radix Paeoniae Rubra part
Experimental example 1
Cell toxicity exploration of red peony part
RBL-2H3 cells in the logarithmic growth phase are taken out of the incubator and digested to prepare a cell suspension. Using a hand-held cytometer (model number): scepter) to calculate cell density. Cell number was adjusted to 1X 10 with fresh complete medium 5 Inoculating 200 mu L of each well into a 96-well plate, uniformly mixing three wells per well, culturing for 24 hours, discarding the supernatant, adding drugs to be screened with different concentrations (0.2 mu g/ml, T2 of 2 mu g/ml and T3 of 20 mu g/ml) prepared by fresh culture medium, adding normal groups (cell blank holes without drugs) and zeroing holes (cell blank holes without drugs), culturing for 24 hours, discarding the supernatant, adding 200 mu L of MTT solution (serum-free culture medium: 5mg/ml: MTT=1:10) prepared by serum, incubating for 4 hours, centrifuging for 400g/5 minutes, discarding the supernatant, adding 150 mu L of DMSO, sufficiently shaking to dissolve crystals, and measuring the OD value of each hole at 570 nm.
Cell viability (%) = (drug group OD value-zeroing group OD value)/(normal group OD value-zeroing group OD value) ×100%
The results are shown in Table 1. As shown in Table 1, the viability of the RBL-2H3 cells was higher than 95% after the administration of each drug, and thus, the cytotoxicity of the red peony portion was small, and it was considered that the red peony portion was not cytotoxic at the administration dose of 20. Mu.g/ml. From the above results, the red peony root has low cytotoxicity and high safety. The dose selection range of 0.2-20 mug/ml can be selected as the dosing range, and the influence of the part on antigen-induced RBL-2H3 cell activation degranulation HIS and beta-HEX release rate can be further discussed.
TABLE 1 cytotoxic Effect of red peony on RBL-2H3 cells (mean.+ -. Standard deviation, n=6)
Experimental example 2
Study of the Effect of radix Paeoniae Rubra on the degranulation of RBL-2H3 cells caused by anaphylaxis
Digesting cells in logarithmic growth phase, and adjusting cell density to 1×10 5 And each ml. 200. Mu.L/well was added to a 96-well plate, and zeroing wells, blank wells, total enzyme wells, and each dosing well were set. The administration wells were divided into model control group, T1, T2 and T3 groups, wherein the final concentration of T1 administration was 0.2. Mu.g/ml, T2 is 2. Mu.g/ml and T3 is 20. Mu.g/ml. Incubating overnight, adding complete culture medium into zeroing well, normal well and total enzyme well for normal culture, adding 200. Mu.L of anti-DNP-IgE prepared from complete culture medium with final concentration of 750ng/mL into model group, adding 200. Mu.L of anti-DNP-IgE prepared from complete culture medium with final concentration of 750ng/mL into dosing well, incubating for 24h, centrifuging, adding modified desktop liquid, washing to no-residue culture medium, adding 200. Mu.L of blank modified desktop liquid into zeroing well and blank control well, adding 200. Mu.L of 1% Triton X-100 lysate into total enzyme well, adding DNP-BSA prepared from modified desktop liquid into dosing group and model control group, centrifuging for 3000r/5min after culturing for 2h, taking supernatant, measuring release amount of histamine and beta-aminoglycosidase, and observing cell morphology by microscope. The histamine release amount measurement method was as follows: taking 100 mu L of cell supernatant, adding 20 mu L of histamine substrate, adding 20 mu L of NaOH, incubating for 15min at 37 ℃, adding 3% HCL solution of stop solution to stop reaction, stabilizing for 15min, and measuring fluorescence values of each group at the excitation wavelength of 355nm and the emission wavelength of 460 nm. From the measured fluorescence values of each group, the histamine release rate was calculated as follows:
histamine release rate (%) = (sample supernatant fluorescence value-zeroed supernatant fluorescence value)/(total enzyme well fluorescence value-zeroed supernatant fluorescence value) ×100%
The method for measuring the release amount of the beta-aminoglycosidase comprises the following steps: the cell supernatant was taken at 50. Mu.L, a substrate of beta-aminoglycosidase was added, incubated at 37℃for 45min, stop reaction was stopped at 200. Mu.L by adding stop solution NaHCO3/Na2CO3, and absorbance of each well was measured at 405 nm. From the OD values measured in each group, the beta-aminoglycosidase release rate was calculated according to the following formula
Beta-aminoglycosidase release (%) = (sample supernatant value-zeroing value)/(total enzyme Kong Zhi-zeroing value) ×100%
As shown in Table 2, the results of the histamine and beta-aminoglycosidase release rate assays indicate that 750ng/mL of anti-DNP-IgE stimulation, 1. Mu.g/mL of DNP-BAS stimulation can significantly increase the content of histamine and beta-aminoglycosidase in cell culture supernatant (P <0.001 ), while the above red peony compound can inhibit the release of histamine and beta-aminoglycosidase at 20. Mu.g/mL (P <0.001 ).
TABLE 2 Effect of red peony on cell degranulation due to anaphylaxis (0.2. Mu.g/mL, 2. Mu.g/mL, 20. Mu.g/mL, mean.+ -. Standard deviation, n=6)
### P<0.001 vs. blank group, ×p<0.05，**P<0.01，***P<0.001vs model control group
Experimental example 3
Effect of radix Paeoniae Rubra on cell degranulation due to anaphylaxis (0.2 μg/mL, 2 μg/mL, 20 μg/mL, mean.+ -. Standard deviation, n=6)
In the 96-well plate, zero-setting holes, blank control holes, total enzyme holes and each administration hole are arranged. The administration holes are divided into model control group and T 1 、T 2 And T 3 Group, wherein T 1 The final concentration of administration is 0.2 mug/mL, T 2 2 μg/mL, T 3 20. Mu.g/mL. Model control group added modified bench top solution of C48/80 solution with a final concentration of 15. Mu.g/ml. Drug administration holes are respectively added with each concentration of drug prepared by the improved desk type liquid and the final concentration is 15 mug/ml C48/80, 200 mu L of the improved desk type liquid is added into zero-setting holes and blank control holes, 200 mu L of 1% TritonX-100 lysate is added into total enzyme holes, supernatant is obtained through centrifugation at 3000r/5min after incubation for 1h, and the release amount of histamine and beta-aminoglycosidase is measured. The measurement method is the same as above.
As shown in Table 3, the results of measurement of histamine and beta-aminoglycosidase release rates show that C48/80 of 15 μg/mL can significantly raise the content of histamine and beta-aminoglycosidase in cell culture supernatant (P <0.001 ), while the above radix Paeoniae Rubra compound can selectively inhibit the release of histamine or beta-aminoglycosidase in the dosage range of 2-20 μg/mL. Wherein histamine: at a dose of 20 μg/mL, its release may be significantly reduced (P < 0.001). For beta-aminoglycosidases: at the dose of 2 mug/mL, the release amount can be obviously reduced (P < 0.001).
TABLE 3 Effect of radix Paeoniae Rubra sites on cell degranulation due to anaphylactic reaction (0.2. Mu.g/mL, 2. Mu.g/mL, 20. Mu.g/mL, mean.+ -. Standard deviation, n=6)
### P<0.001 vs. blank group, ×p<0.05，**P<0.01，***P<As is clear from the results of Experimental example 2 and Experimental example 3, the 0.001vs model control group had no antiallergic or antiallergic effects at each of the doses administered, CS-1, CS-2 and CS-3. Only CS-4 at the red peony site significantly inhibited the release of histamine and beta-aminoglycosidase in allergic and anaphylactoid-induced cell degranulation at a dose of 20. Mu.g/mL (P<0.001). Thus, the CS-4 site was further extracted and isolated.
3. Pharmacological Activity experiment of Red peony root monomer Compound
The research at home and abroad shows that common allergens in nature such as pollen, dust, catkin, animal fur, lampblack, food, medicine and the like appear in various links of life, and the allergens can cause organisms to be allergic or anaphylactic reaction. The pharmacological experiments show that the compounds I, II, III and IV have obvious effect of inhibiting the release of histamine and beta-hexosaminidase, and can be used for preparing medicines for preventing or treating allergic diseases.
Experimental example 4
Red peony compound cytotoxicity search
RBL-2H3 cells in the logarithmic growth phase are taken out of the incubator and digested to prepare a cell suspension. Cell density was calculated using a hand-held cell counter (model: scepter). Cell number was adjusted to 1X 10 with fresh complete medium 5 Inoculating 200 mu L of each well into a 96-well plate, uniformly mixing three wells for 24 hours, discarding the supernatant, adding medicines to be screened with different concentrations (0.08, 0.2, 4, 10 and 50 mu M) prepared by fresh culture medium into each group of 3 compound wells, additionally arranging a normal group (cell blank hole without medicine) and a zeroing hole (cell blank hole without medicine), discarding the supernatant after culturing for 24 hours, adding 200 mu L of MTT solution with serum-free configuration (serum-free culture medium: 5mg/ml: MTT=1:10), incubatingAfter 4h incubation, 400g/5min centrifugation, removal of supernatant, addition of 150. Mu.L DMSO, sufficient shaking to dissolve the crystals, and measurement of OD at 570nm for each well.
Cell viability (%) = (drug group OD value-zeroing group OD value)/(normal group OD value-zeroing group OD value) ×100%
The results are shown in Table 1. As shown in Table 1, after each drug was applied to RBL-2H3 cells, the cell viability was higher than 80%, and thus, it was found that the red peony root compound was less cytotoxic and highly safe. The dosage selection range of 0.08-50 mu M can be selected as the administration range, and the influence of each drug on antigen-induced RBL-2H3 cell activation degranulation His and beta-HEX release rate is further discussed.
TABLE 4 cytotoxic Effect of Red peony compounds on RBL-2H3 cells (mean.+ -. Standard deviation, n=6)
Experimental example 5
Investigation of the Effect of Red peony root Compounds on the degranulation of RBL-2H3 cells by anaphylaxis
Digesting cells in logarithmic growth phase, and adjusting cell density to 1×10 5 And each ml. 200. Mu.L/well was added to a 96-well plate, and zeroing wells, blank wells, total enzyme wells, and each dosing well were set. The administration holes are divided into model control group and T 1 、T 2 And T 3 Group, wherein T 1 The final concentration of administration is 50 mu M, T 2 25 mu M, T 3 5. Mu.M. Incubating overnight, adding complete medium into zeroing hole, normal hole and total enzyme hole for normal culture, adding 200 μl of anti-DNP-IgE prepared from complete medium with final concentration of 750ng/mL into model group, adding 200 μl of anti-DNP-IgE prepared from each concentration of drug and final concentration of 750ng/mL into administration hole, incubating for 24 hr, centrifuging, adding improved desk liquid, cleaning to no residual medium, adding 200 μl of blank improved desk liquid into zeroing hole and blank control hole, adding 200 μl of 1% Triton X-100 lysate into total enzyme hole, adding 200 μl of DNP-BSA prepared from improved desk liquid into administration group and model control group, centrifuging 3000r/5min after culturing for 2 hr to obtain supernatant, and measuring groupAmine and beta-hexosaminidase release amounts and cell morphology was observed microscopically. The histamine release amount measurement method was as follows: taking 100 mu L of cell supernatant, adding 20 mu L of histamine substrate, adding 20 mu L of NaOH, incubating for 15min at 37 ℃, adding 3% HCL solution of stop solution to stop reaction, stabilizing for 15min, and measuring fluorescence values of each group at the excitation wavelength of 355nm and the emission wavelength of 460 nm. From the measured fluorescence values of each group, the histamine release rate was calculated as follows:
histamine release rate (%) = (sample supernatant fluorescence value-zeroed supernatant fluorescence value)/(total enzyme well fluorescence value-zeroed supernatant fluorescence value) ×100%
The method for measuring the release amount of the beta-hexosaminidase comprises the following steps: taking 50 mu L of cell supernatant, adding beta-hexosaminidase substrate, incubating for 45min at 37 ℃, adding stop solution NaHCO 3 /Na 2 CO 3 The reaction was stopped at 200. Mu.L and the absorbance of each well was measured at 405 nm. From the OD values measured in each group, the release rate of beta-hexosaminidase was calculated according to the following formula
Beta-hexosaminidase release (%) = (sample supernatant value-zeroing value)/(total enzyme Kong Zhi-zeroing value) ×100%
Microscopic observation results show that the RBL-2H3 cells of the normal group are long fusiform, complete in edge and compact in structure. The cell volume of the model group is increased, the edge is irregular, a large number of vacuoles or particle-like structures appear, most cell membranes are broken, and particle-like substances are exuded. The cellular state of the red paeony root compound is obviously improved, the vacuole-like structure is obviously reduced, and the red paeony root compound is suggested to be capable of effectively protecting the cellular form to be perfect and inhibiting the exudation of the particle-like substances.
As shown in Table 5, the results of the histamine and beta-aminohexosaminidase release rate measurement show that 750ng/ml of anti-DNP-IgE stimulation and 1. Mu.g/ml of DNP-BAS stimulation can significantly increase the content of histamine and beta-aminohexosaminidase in cell culture supernatant (P <0.001 and P < 0.001), and the red peony root compound can selectively inhibit the release of histamine or beta-aminohexosaminidase in the dosage range of 5-50. Mu.M. Wherein histamine: the release amount of the drug can be obviously reduced at the administration dose of 25 mu M (P <0.001, P <0.01, P <0.001 and P < 0.01). For beta-hexosaminidase: the release amount of the drug can be obviously reduced at the administration dosage of 25 mu M (P <0.001, P <0.05 and P < 0.01). Compound i still significantly reduced the content of β -hexosaminidase in the cell supernatant at a dose of 5 μm (P < 0.05).
TABLE 5 Effect of Red peony root Compounds on cell degranulation due to anaphylaxis (5. Mu.M, 25. Mu.M, 50. Mu.M, mean.+ -. Standard deviation, n=6)
### P<0.001 vs. blank group P<0.05，**P<0.01，***P<0.001vs model control group
Experimental example 6
Effect of Red peony Compound on cell degranulation due to anaphylactoid reaction (50. Mu.M, 25. Mu.M, 5. Mu.M, mean.+ -. Standard deviation, n=6)
In the 96-well plate, zero-setting holes, blank control holes, total enzyme holes and each administration hole are arranged. The administration holes are divided into model control group and T 1 、T 2 And T 3 Group, wherein T 1 The final concentration of administration is 50 mu M, T 2 25 mu M, T 3 5. Mu.M. Model control group added modified bench top solution of C48/80 solution with a final concentration of 15. Mu.g/ml. Drug administration holes are respectively added with each concentration of drug prepared by the improved desk type liquid and the final concentration is 15 mug/ml C48/80, 200 mu L of the improved desk type liquid is added into zero-setting holes and blank control holes, 200 mu L of 1% TritonX-100 lysate is added into total enzyme holes, supernatant is obtained through centrifugation at 3000r/5min after incubation for 1h, and release amounts of histamine and beta-aminohexosaminidase are measured. The measurement method is the same as above.
As shown in Table 6, the results of measurement of histamine and beta-aminohexosaminidase release rates show that C48/80 of 15. Mu.g/ml can significantly raise the content of histamine and beta-aminohexosaminidase in cell culture supernatant (P <0.001 ), while the above-mentioned red peony compound can selectively inhibit the release of histamine or beta-aminohexosaminidase in the dosage range of 5-50. Mu.M. Wherein histamine: at the 50 μm dose, the release amount was significantly reduced (P < 0.001). Wherein compounds I, III still significantly reduce histamine levels in cell supernatants at doses of 5. Mu.M (P <0.001, P < 0.01)). For beta-hexosaminidase: compounds I, III still significantly reduced their release (P < 0.05) at 5. Mu.M.
TABLE 6 Effect of Red peony root Compounds on cell degranulation due to anaphylactoid reaction (5. Mu.M, 25. Mu.M, 50. Mu.M, mean.+ -. Standard deviation, n=6)
### P<0.001 vs. blank group P<0.05，**P<0.01，***P<0.001vs model control group
As can be seen from the results in experimental examples 2 and 3, the compounds I, II, III, IV significantly inhibited the release of histamine and beta-hexosaminidase (P < 0.001) in the cells degranulation caused by allergic reaction and anaphylactoid reaction at the administration dose of 50. Mu.M. Compounds I and II still show a strong antiallergic effect at a dose of 25. Mu.M. Compounds I and III still selectively inhibit histamine and beta-hexosaminidase release at 5. Mu.M doses.
Experimental example 7
Effect of Red peony Compound on TNF-alpha and IL-4 in cell supernatant caused by anaphylaxis
Digesting cells in logarithmic growth phase, and adjusting cell density to 1×10 5 And each ml.500 μl/well was added to the 24-well plate, and zeroing wells, blank wells, and each dosing well were set. The administration holes are divided into model control group and T 1 、T 2 And T 3 Group, wherein T 1 The final concentration of administration is 50 mu M, T 2 25 mu M, T 3 5. Mu.M. Incubating overnight, adding complete culture medium into zeroing well, normal well and total enzyme well for normal culture, adding anti-DNP (anti-DNP) prepared from complete culture medium with final concentration of 750ng/ml into model group200 mu L of IgE, 200 mu L of anti-DNP-IgE with each concentration of drug and final concentration of 750ng/mL are respectively added into a dosing hole, incubation is carried out for 24 hours, centrifugation is carried out, an improved desk-top liquid is added to clean until no residual culture medium exists, 200 mu L of blank improved desk-top liquid is added into a zeroing hole and a blank control hole, DNP-BSA with final concentration of 1 mu g/mL is added into a dosing group and a model control group, after 2 hours of culture, supernatant is centrifugally taken at 3000r/5 minutes, and the content of inflammatory factors TNF-alpha and IL-4 in the supernatant is detected according to the method of the specification of the TNF-alpha and IL-4 kit of rats of Jiangsu Jingmei biotechnology Co.
As shown in Table 7, the results of the assay for the release of the inflammatory mediators TNF- α, IL-4 demonstrate that 750ng/ml of anti-DNP-IgE stimulation, 1 μg/ml of DNP-BAS stimulation significantly increases the levels of TNF- α and IL-4 in the cell culture supernatant (P <0.001 ), while the red peony compound significantly inhibits the release of TNF- α and IL-4 at a dose of 50 μM (P < 0.001). Wherein TNF- α: compound i still significantly inhibited its content in the supernatant (P < 0.05) at a dose of 5 μm. IL-4: compounds I, II, III, IV all significantly inhibited IL-4 content in the supernatant at 25. Mu.M dose (P <0.001, P <0.01, P <0.001, P < 0.05). Compound i still significantly inhibited the content of IL-4 in the supernatant (P < 0.05) at a dose of 5 μm.
TABLE 7 Effect of Red peony Compound on TNF- α and IL-4 in cell supernatants due to anaphylaxis (5. Mu.M, 25. Mu.M, 50. Mu.M, mean.+ -. Standard deviation, n=6)
### P<0.001 vs. blank group P<0.05，**P<0.01，***P<0.001vs model control group
Experimental example 8
Effect of Red peony Compound on TNF-alpha and IL-4 in cell supernatants caused by anaphylactoid reactions
Digesting cells in logarithmic growth phase, and adjusting cell density to 1×10 5 And each ml.500 μl/well was added to the 24-well plate, and zeroing wells, blank wells, and each dosing well were set. The administration holes are divided into model control group and T 1 、T 2 And T 3 Group, wherein T 1 The final concentration of administration is 50 mu M, T 2 25 mu M, T 3 5. Mu.M. Model control group added modified bench top solution of C48/80 solution with a final concentration of 15. Mu.g/ml. The other drug administration wells were filled with each concentration of drug prepared with the modified tabletop liquid and a final concentration of 15 μg/ml C48/80 of 200 μl, and the zeroing well and the blank control well were filled with 200 μl of blank modified tabletop liquid. After incubation for 1h, the supernatant was centrifuged at 3000r/5min, and the content of inflammatory factors TNF-alpha and IL-4 in the supernatant was detected according to the method described in the kit of rat TNF-alpha and IL-4 from Jiangsu Mei Biotech.
As shown in Table 8, the results of the detection of the release of the inflammatory mediators TNF-alpha and IL-4 show that 15 mug/ml of C48/80 can significantly increase the content of TNF-alpha and IL-4 in the cell culture supernatant (P <0.001 ), while the red paeony root compound can significantly inhibit the release of TNF-alpha and IL-4 under the action of 50 mug dosage (P < 0.001). Wherein TNF- α: compound i still significantly inhibited its content in the supernatant (P < 0.05) at a dose of 5 μm. IL-4: compounds II and III both significantly inhibited IL-4 content in cell supernatants at doses of 5. Mu.M (P <0.01, P < 0.05).
TABLE 8 Effect of Red peony Compound on TNF- α and IL-4 in cell supernatants due to anaphylactoid reaction (5. Mu.M, 25. Mu.M, 50. Mu.M, mean.+ -. Standard deviation, n=6)
### P<0.001 vs. blank group P<0.05，**P<0.01，***P<0.001vs model control group
As is clear from experimental examples 7 and 8, the red peony root compound can selectively inhibit the contents of TNF-alpha and IL-4 in the supernatant of RBL-2H3 cells, and has good anti-inflammatory effect. From experimental examples 5 and 6, it is known that the red peony root compound can significantly inhibit the degranulation of RBL-2H3 cells caused by anaphylaxis and anaphylactoid reaction, reduce the release of cell histamine and beta-hexosaminidase, and has good antiallergic and antiallergic effects. As is clear from example 4 and Experimental example 1, the above red peony site and red peony compound are high in safety. From the results in experimental examples 2 and 3, it was found that the red peony site significantly inhibited the release of histamine and β -aminoglycosidase in cell degranulation due to allergic reaction and anaphylactoid reaction (P < 0.001) at the dose of 20 μg/mL. The concentrations of the common significant difference in the effect of the red peony monomer compounds are 25 mu M/L, the relative molecular masses of the four red peony compounds are 362, 376, 360 and 378 respectively, the highest concentration of the effect of the compounds after the conversion unit is 9.05 mu g/mL,9.40 mu g/mL,9.00 mu g/mL and 9.45 mu g/mL respectively, and the concentration is less than the dosage of the administration part of 20 mu g/mL, so the antiallergic and allergy-like effects of the red peony monomer compounds are stronger than those of the red peony part (CS-4)
Therefore, the experimental results show that the lignin red paeony root compound has higher safety, good antiallergic, antiallergic and anti-inflammatory activities, and the red paeony root monomer has stronger activity compared with the part.
Note that: the allergic reaction is divided into two stages, a stimulation stage and a stimulation stage, and in the experiment, the allergic reaction positive medicine is divided into an exciting agent and a stimulation, wherein the exciting agent is anti-DNP-IgE, and the Chinese name is anti-dinitrophenol IgE monoclonal antibody. The excitant is DNP-BSA, and the Chinese name is dinitrophenol-bovine serum albumin; the positive medicine of the anaphylactic reaction is C48/80, and belongs to mast cell activators.
Claims (4)
1. The application of lignin compound or pharmaceutically acceptable salt thereof in preparing medicines for preventing, relieving and/or treating allergic diseases is characterized in that the lignin compound is selected from one or more of the following groups,
2. the use according to claim 1, wherein the pharmaceutically acceptable salt of the compound is selected from the group consisting of salts of the compound with inorganic or organic acids.
3. The use according to claim 1 or 2, wherein the medicament for preventing, alleviating and/or treating allergic diseases comprises a therapeutically effective amount of the compound or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable carrier.
4. The use according to claim 3, wherein the dosage form of the medicament for preventing, alleviating and/or treating allergic diseases is selected from the group consisting of tablets, capsules, pills, granules, oral liquids, eye drops and inhalants.
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| KR20170055371A (en) * | 2015-11-11 | 2017-05-19 | 대구가톨릭대학교산학협력단 | A composition comprising compounds isolated from Echinochloa utilis for preventing or treating inflammatory disease |
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