CN112971789B - Extensible flexible electrode transfer method based on elastic seal containing fluid channel - Google Patents
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Abstract
The invention discloses a transfer method of an extensible flexible electrode based on an elastic seal with a fluid channel, which comprises the steps of firstly, pressing the elastic seal above the extensible flexible electrode, and injecting a water-soluble rapid degradation material solution into the fluid channel; then heating and drying the solution, and adhering and lifting the extensible flexible electrode from the glass substrate by the elastic seal; then transferring the elastic seal with the extensible flexible electrode adhered on the bottom surface to the upper part of the target brain region by utilizing a micro-motion platform, and injecting artificial cerebrospinal fluid into the fluid channel to gradually dissolve the degradation material; finally, the material to be degraded is completely dissolved, and the elastic seal is separated from the extensible flexible electrode, so that the extensible flexible electrode is stably attached to the surface of the cerebral cortex. The method has very important practical value and innovative significance for accurately acquiring the stable cortex brain electrical signals of the target brain region coordinates, and can effectively solve the problems that the existing electrode point discretization extensible flexible electrode attaching operation is difficult and the relative position of the electrode point is not easy to control.
Description
Technical Field
The invention belongs to the technical field of biomedical engineering, and particularly relates to a transfer method of an extensible flexible electrode.
Background
The development and fusion of micro-electro-mechanical system (MEMS) technology and flexible electronic technology provide important guarantee for the development of high-precision brain science research tools. Among them, the cortical brain electrode is a very important class, and in order to improve the adhesion degree between the electrode and the surface of the sulcus-shaped cortex, a strip electrode or a grid electrode of a flexible polymer substrate is often used. However, due to the micro-motion of the combination of high frequency blood vessels and low frequency respiratory pulsations, and brain motion caused by body motion, there are higher demands on the compliance of the electrodes.
The flexible polymer substrate electrode has good machining performance, and is currently the mainstream scheme for realizing high-precision and high-density electrodes. In order to further improve the compliance of the flexible polymer substrate electrode with brain tissue micromotion, the flexible polymer substrate electrode can be realized through discretization and extensible structural design. However, discretized and malleable structures tend to result in relative positional misalignment between the individual electrodes in the form of wires or strips, especially during implantation, which increases the difficulty of handling. Therefore, developing a high-precision transfer method of the extensible flexible cortical brain electrode has important significance in ensuring the precision of the implantation position.
In the prior art, kim D H, vivanti J et al, nature materials,2010,9 (6): 511-517, written "Dissolvable films of silk fibroin for ultrathin conformal bio-integrated electronics", a reticulated polyimide-backed flexible cortical brain electrode of thickness only 2.5 microns was bonded to a silk fibroin film prepared by cast drying, integrally transferred to the cat's cerebral cortex surface, and the silk fibroin film was dissolved, thereby conformally attaching the electrode to the cortical surface. The silk fibroin is used as a temporary hardening layer, so that the electrode can be in a flat state in the transfer process, but certain difficulty still exists in ensuring accurate transfer to the target cortex coordinate.
Huang J, wang L et al Advanced Functional Materials,2020,30 (23): 2001518 written "Tuning the rigidity of silk fibroin for the transfer of highly stretchable electronics", which adopts a silk fibroin material doped with calcium ions with variable rigidity as a seal, and the elastic modulus of the seal material is kept between 100MPa and 1.84GPa under the condition of low humidity (the relative humidity is within the range of 33% -49%) for transferring the flexible electronic material; after peeling, the silk fibroin is placed in a high-humidity environment to soften the silk fibroin, the elastic modulus of the silk fibroin is reduced to 0.1MPa to 2MPa, and the silk fibroin is equivalent to skin, and can be directly used as a surface electrode. However, the method can form constraint on the patterned conductive material by softening the silk fibroin and taking the silk fibroin as a stretchable substrate, is unfavorable for the conformal contact between the discrete patterned conductive material and the cerebral cortex sulcus, and has the problem of transfer precision.
CN109645991a discloses an intelligent intracranial plasma electrode and a method for accurately collecting cortex electroencephalogram, which are connected at the comb back of a comb-shaped flexible substrate, separated at the comb teeth, electrode points are distributed on parallel flexible substrates, direct transfer attachment is adopted, then an intelligent electrode contact state detector is used for detecting the electrode placement state in real time, a doctor is prompted to find an unset electrode so as to adjust in time, the accuracy of the electrode transfer position is not high, and meanwhile, the comb-shaped electrode is flexible and does not have extensibility.
CN110251125 a discloses a flexible stretchable nerve electrode, a preparation method and application thereof, which comprises a flexible polymer material, a liquid metal electrode embedded in the flexible polymer material and an insulating material, wherein the flexible polymer electrode has stretching performance, but the flexible polymer substrate is still a whole piece, and the electrode points are constrained by the substrate, so that the whole is not beneficial to ensuring the conformal contact between the whole and the cortex sulcus of the brain, and meanwhile, the method and the precision problem of transferring the electrode to the nerve tissue are not mentioned.
CN110604566a discloses a flexible deformable degradation brain detection treatment device, system and manufacturing and using method, comprising a serpentine dielectric layer and a functional layer, and a whole piece of shape memory polymer substrate, which is also ductile, but the whole piece of shape memory polymer substrate still causes constraint between electrode points, which is unfavorable for conformal attachment, and a detailed method for transferring electrodes to cerebral cortex is not mentioned.
From the above results, most of the existing researches are focused on flexible electrode materials and structures, and a method for transferring a flexible electrode with high precision and attaching a target brain region is lacking, and the traditional attachment is only carried out manually, so that the position precision is difficult to ensure. Especially for discretized ductile flexible electrodes, wire-like or ribbon-like electrode position disorder is very easy to occur during operation, and it is difficult to ensure that the electrode points remain in relatively fixed positions.
Therefore, a high-precision transfer method of the extensible flexible electrode with the discretized electrode points is developed, has very important practical value and innovative significance for accurately acquiring stable cortex brain electrical signals of target brain region coordinates, and can effectively solve the problems that the conventional flexible electrode with the discretized electrode points is difficult to attach and the relative positions of the electrode points are not easy to control.
Disclosure of Invention
In order to overcome the defects in the prior art, the invention provides a transfer method of an extensible flexible electrode based on an elastic seal with a fluid channel, which comprises the steps of firstly pressing the elastic seal above the extensible flexible electrode, and injecting a water-soluble rapid degradation material solution into the fluid channel; then heating and drying the solution, and adhering and lifting the extensible flexible electrode from the glass substrate by the elastic seal; then transferring the elastic seal with the extensible flexible electrode adhered on the bottom surface to the upper part of the target brain region by utilizing a micro-motion platform, and injecting artificial cerebrospinal fluid into the fluid channel to gradually dissolve the degradation material; finally, the material to be degraded is completely dissolved, and the elastic seal is separated from the extensible flexible electrode, so that the extensible flexible electrode is stably attached to the surface of the cerebral cortex. The method has very important practical value and innovative significance for accurately acquiring the stable cortex brain electrical signals of the target brain region coordinates, and can effectively solve the problems that the existing electrode point discretization extensible flexible electrode attaching operation is difficult and the relative position of the electrode point is not easy to control.
The technical scheme adopted by the invention for solving the technical problems comprises the following steps:
step 1: the method comprises the steps of covering an elastic seal with a fluid channel above an extensible flexible electrode, and injecting a water-soluble rapid degradation material solution into the fluid channel of the elastic seal with the fluid channel, wherein the water-soluble rapid degradation material solution is contacted with the extensible flexible electrode through a circular hole array on the bottom surface of the elastic seal with the fluid channel;
step 2: placing the elastic seal containing the fluid channel, the extensible flexible electrode and the glass substrate into an oven for heating, and hardening the water-soluble rapid degradation material solution; taking the elastic seal containing the fluid channel, the extensible flexible electrode and the glass substrate out of the oven, and then adhering the extensible flexible electrode to the bottom surface of the elastic seal containing the fluid channel through a hardened water-soluble rapid degradation material solution, wherein the extensible flexible electrode is separated from the glass substrate;
step 3: moving the fluid channel-containing elastic seal with the extensible flexible electrode adhered on the bottom surface to the upper part of a target brain region by utilizing a micro-motion platform, moving the fluid channel-containing elastic seal downwards to be in elastic contact with the cerebral cortex, and injecting artificial cerebrospinal fluid into the fluid channel containing the fluid channel-containing elastic seal to gradually dissolve the hardened water-soluble rapid degradation material solution;
step 4: the water-soluble rapid degradation material solution to be hardened is completely dissolved, and the elastic seal containing the fluid channel is lifted and separated from the extensible flexible electrode, so that the extensible flexible electrode is attached to the surface of the cerebral cortex.
Preferably, the elastic seal containing the fluid channel is made of a highly transparent elastomer material, in particular polydimethylsiloxane or polyurethane.
Preferably, the dimension of the elastic stamp containing fluid channels is 3mm×3mm or 20mm×20mm.
Preferably, the water-soluble rapid degradation material is one of sodium alginate, polyethylene glycol, polyvinyl alcohol and sucrose.
Preferably, the ductile flexible electrode comprises a polymer substrate layer, a conductive functional layer, and a polymer encapsulation layer, the conductive functional layer being encapsulated intermediate the polymer substrate layer and the polymer encapsulation layer; the extensible flexible electrode is close to the area where the electrode point and the brain directly contact and act, and adopts a discrete snake-shaped structure.
Preferably, the material of the polymer substrate layer and the polymer packaging layer is polyimide, and the material of the conductive functional layer is gold or platinum.
Preferably, the material of the polymer substrate layer and the polymer packaging layer is parylene, and the material of the conductive functional layer is a transparent conductive material compounded by poly 3, 4-ethylenedioxythiophene/polystyrene sulfonate and single-walled carbon nanotubes.
Preferably, the temperature of heating in the oven in the step 2 is 70-80 ℃ and the heating time is 1-4 hours.
Preferably, the elastic seal with the fluid channel is divided into an upper part and a lower part, wherein the upper part consists of a hollow cylindrical handle and a rectangular inner concave cavity body, and the hollow part of the hollow cylindrical handle is a fluid channel; the lower part is a cuboid which can be embedded into the upper rectangular concave cavity, one surface of the cuboid is provided with a groove, the other surface is provided with a round hole array, and the round holes are through holes; and injecting liquid into the fluid channel, enabling the liquid to flow to the rectangular concave cavity along the fluid channel, and enabling the liquid to flow out of the elastic seal containing the fluid channel through the circular hole array.
A processing method of an elastic seal with a fluid channel comprises the following steps:
step 1: processing the upper part of the elastic seal containing the fluid channel by using a 3D printing resin material rapid forming die, injecting liquid PDMS into a cavity from an annular inlet at the upper end of the die by using an injector, heating in an oven at 60 ℃ for 2 hours, and taking out the upper part of the elastic seal containing the fluid channel from the die after the PDMS is not completely solidified;
step 2: the lower part of the elastic seal containing the fluid channel is processed by using a rectangular groove die with a cylindrical array formed by 3D printing resin material, liquid PDMS is poured into the die, the liquid level is not higher than the cylindrical array, the liquid level is heated in a box at 60 ℃ for 2 hours, and the lower part of the elastic seal containing the fluid channel is taken out from the die after the PDMS is not solidified completely;
step 3: and (3) activating the surfaces of the PDMS of the upper part and the lower part of the elastic seal containing the fluid channel by oxygen plasma, then immediately aligning and bonding, and putting the PDMS into an oven at 80 ℃ to heat for 2 hours, so that complete solidification is realized, and firm bonding between the two parts is ensured.
The beneficial effects of the invention are as follows:
the invention provides a transfer method of an extensible flexible electrode based on a fluid channel elastic seal, wherein the position of the electrode can be clearly observed through a highly transparent seal, and then the seal is controlled by a micro-motion platform to be accurately moved to the position above a target brain region together with the electrode. Meanwhile, in order to improve the reliability of the transfer process, the electrode is adhered to the bottom surface of the seal by using a hardened water-soluble rapid degradation material, and is rapidly softened and dissolved by cerebrospinal fluid injected through a fluid channel, so that the electrode is rapidly separated from the seal and stably adhered to a target cortex region under the action of seal pressure, and the problem of repeated adhesion and uncovering repositioning caused by inaccurate positions of the current extensible flexible electrode is effectively solved.
Drawings
FIG. 1 is a schematic illustration of a method of transferring a malleable flexible electrode based on a flexible stamp containing a fluid channel in accordance with the present invention.
FIG. 2 is a schematic diagram of a method of processing an elastic stamp with a fluid channel according to the present invention.
FIG. 3 is a schematic view of a layered structure of a fluid channel-containing elastomeric stamp and a malleable flexible electrode, in accordance with an embodiment of the present invention.
FIG. 4 is a schematic illustration of the relative positions of a fluid channel-containing elastomeric stamp and a malleable flexible electrode, in accordance with an embodiment of the present invention.
In the figure: the liquid-containing elastic seal comprises a 1-elastic seal with a fluid channel, a 2-extensible flexible electrode, a 3-glass substrate, a 4-water-soluble rapid degradation material solution, 5-artificial cerebrospinal fluid, 6-cerebral cortex, an upper part of the 7-elastic seal, a lower part of the 8-elastic seal, a 9-polymer substrate layer, a 10-conductive functional layer and an 11-polymer packaging layer.
Detailed Description
The invention will be further described with reference to the drawings and examples.
As shown in fig. 1, the method for transferring the extensible flexible electrode based on the elastic seal with the fluid channel comprises the following steps:
step 1: the method comprises the steps that a fluid channel-containing elastic seal 1 is covered above a ductile flexible electrode 2, a water-soluble rapid degradation material solution 4 is injected into a fluid channel of the fluid channel-containing elastic seal 1, and the water-soluble rapid degradation material solution 4 is contacted with the ductile flexible electrode 2 through a circular hole array on the bottom surface of the fluid channel-containing elastic seal 1;
step 2: placing the elastic seal 1 containing the fluid channel, the extensible flexible electrode 2 and the glass substrate 3 into an oven for heating, and hardening the water-soluble rapid degradation material solution 4; after the elastic seal 1 with the fluid channel, the extensible flexible electrode 2 and the glass substrate 4 are taken out of the oven, the extensible flexible electrode 2 is adhered to the bottom surface of the elastic seal 1 with the fluid channel through the hardened water-soluble rapid degradation material solution 4, and the extensible flexible electrode 2 is separated from the glass substrate 3;
step 3: moving the fluid channel-containing elastic seal 1 with the extensible flexible electrode 2 adhered to the bottom surface to the upper part of a target brain region by utilizing a micro-motion platform, moving the fluid channel-containing elastic seal 1 downwards to be in elastic contact with the cerebral cortex 6, and injecting artificial cerebrospinal fluid 5 into the fluid channel of the fluid channel-containing elastic seal 1 to gradually dissolve the hardened water-soluble rapid degradation material solution 4;
step 4: the water-soluble rapid degradation material solution 4 to be hardened is completely dissolved, the elastic seal 1 with the fluid channel is lifted, and the elastic seal 1 with the fluid channel is separated from the extensible flexible electrode 2, so that the extensible flexible electrode 2 is attached to the surface of the cerebral cortex 6.
Preferably, the fluid-channel-containing elastic stamp 1 is made of a highly transparent elastomer material, in particular polydimethylsiloxane or polyurethane.
Preferably, the dimension of the elastic stamp 1 containing fluid channels is 3mm×3mm or 20mm×20mm.
Preferably, the water-soluble rapid degradation material 4 is one of sodium alginate, polyethylene glycol, polyvinyl alcohol and sucrose.
Preferably, the ductile flexible electrode 2 comprises a polymer substrate layer 9, a conductive functional layer 10 and a polymer encapsulation layer 11, the conductive functional layer 10 being encapsulated in between the polymer substrate layer 9 and the polymer encapsulation layer 11; the extensible flexible electrode 2 is close to the area where the electrode point directly contacts with the brain and is in a discrete snake-shaped structure.
Preferably, the material of the polymer substrate layer 9 and the polymer packaging layer 11 is polyimide, and the material of the conductive functional layer 10 is gold or platinum.
Preferably, the material of the polymer substrate layer 9 and the polymer packaging layer 11 is parylene, and the material of the conductive functional layer 10 is a transparent conductive material compounded by poly 3, 4-ethylenedioxythiophene/polystyrene sulfonate and single-walled carbon nanotubes.
Preferably, the temperature of heating in the oven in the step 2 is 70-80 ℃ and the heating time is 1-4 hours.
Preferably, the elastic seal 1 with the fluid channel is divided into an upper part and a lower part, the upper part 7 consists of a hollow cylindrical handle and a rectangular inner concave cavity, and the hollow part of the hollow cylindrical handle is a fluid channel; the lower part 8 is a cuboid which can be embedded into the upper rectangular concave cavity, one surface of the cuboid is provided with a groove, the other surface is provided with a round hole array, and the round holes are through holes; liquid is injected into the fluid channel, flows to the rectangular concave cavity along the fluid channel, and flows out of the elastic seal 1 containing the fluid channel through the circular hole array.
As shown in fig. 2, a processing method of the elastic seal with the fluid channel comprises the following steps:
step 1: processing the upper part 7 of the elastic seal 1 containing the fluid channel by using a 3D printing resin material rapid forming die, injecting liquid PDMS into a cavity from an annular inlet at the upper end of the die by using an injector, heating for 2 hours at 60 ℃ in an oven, and taking out the upper part 7 of the elastic seal 1 containing the fluid channel from the die after the PDMS is not solidified completely;
step 2: the lower part 8 of the elastic seal 1 with the fluid channel is processed by using a rectangular groove die with a cylindrical array formed by 3D printing resin material; pouring liquid PDMS into a mould, enabling the liquid level not to be higher than the cylinder array, heating for 2 hours at 60 ℃ in the box, and taking out the lower part 8 of the elastic seal 1 containing the fluid channel from the mould when the PDMS is not completely solidified;
step 3: the PDMS surfaces of the upper part 7 and the lower part 8 of the elastic stamp 1 containing the fluid channel are activated by oxygen plasma, then are immediately aligned and bonded, and are put into an oven at 80 ℃ to be heated for 2 hours, so that complete solidification is realized, and firm bonding between the two parts is ensured.
Specific examples:
the extensible flexible electrode transfer process based on the fluid channel elastic seal mainly comprises the following four steps:
the method comprises the steps of firstly, covering an elastic seal 1 containing a fluid channel above an extensible flexible electrode 2, wherein exposed electrode points of the extensible flexible electrode 2 are downwards paved on the surface of a glass substrate 3, injecting a water-soluble rapid degradation material solution 4 into the fluid channel, and enabling the solution to contact with the extensible flexible electrode 2 through array small round holes at the bottom of the elastic seal 1 containing the fluid channel;
the water-soluble rapid degradation material solution 4 is polyethylene glycol (PEG) solution, and the injection quantity is related to the size of the elastic seal 1 with the fluid channel, and is in standard of being capable of uniformly contacting with the extensible flexible electrode 2 through the array type small round holes;
secondly, heating the whole body in an oven at 70-80 ℃ for 1-4 hours, drying the PEG solution contacted with the extensible flexible electrode 2 and the PEG solution in the fluid channel of the fluid channel-containing elastic seal 1 to form a hardening layer, and flatly adhering and lifting the extensible flexible electrode 2 from the surface of the glass substrate 3 by the fluid channel-containing elastic seal 1;
thirdly, observing through a stereoscopic microscope, clamping and fixing the upper end cylindrical part of the fluid channel containing elastic seal 1 on a micro-motion platform comprising XYZ axis movement, tilting and rotation, transferring the fluid channel containing elastic seal 1 with the extensible flexible electrode 2 adhered on the bottom surface to the upper part of a target cerebral cortex 6, downwards moving to slightly and elastically contact with cerebral tissue, and injecting artificial cerebrospinal fluid 5 into the fluid channel to gradually dissolve the hardened PEG;
and fourthly, completely dissolving the PEG to be hardened, slightly lifting the elastic seal 1 containing the fluid channel, separating the elastic seal from the extensible flexible electrode 2, and stably attaching the extensible flexible electrode 2 to the surface of the cerebral cortex 6 so as to finish the transfer.
Referring to fig. 2, the elastic stamp 1 with a fluid channel is divided into an upper part and a lower part, in this embodiment, PDMS is used for both the upper part and the lower part, and the processing procedure is as follows:
firstly, a 3D printing resin material rapid forming die is used for the upper part of the elastic seal 1 containing the fluid channel, liquid PDMS is injected into a cavity from an annular inlet at the upper end of the die, the liquid PDMS is heated in an oven at 60 ℃ for 2 hours, and the upper part of the elastic seal 1 containing the fluid channel is taken out from the die after the PDMS is not completely solidified. Structurally, the upper part mainly comprises a cylindrical hole channel and a rectangular concave cavity. Secondly, a rectangular groove mould with a 4 multiplied by 4 array cylinder is formed by using a 3D printing resin material, liquid PDMS is poured into the mould, the liquid level is not higher than the array cylinder, the liquid level is heated for 2 hours at 60 ℃ in a box, and the lower part of the elastic seal 1 containing the fluid channel is taken out from the mould after the PDMS is not solidified completely. And finally, carrying out activation treatment on the surfaces of the two parts of PDMS by oxygen plasma, immediately aligning and bonding, and putting the two parts of PDMS into an oven at 80 ℃ to heat for 2 hours to realize complete solidification and ensure firm bonding between the two parts.
Referring to fig. 3, there is shown a layered structure and relative positions of a fluid channel-containing elastic stamp 1 and a ductile flexible electrode 2, wherein the fluid channel-containing elastic stamp 1 includes an elastic stamp upper portion 7 and an elastic stamp lower portion 8, and the ductile flexible electrode 2 includes a polymer substrate layer 9, a conductive functional layer 10, and a polymer encapsulation layer 11. In this embodiment, the material of the polymer substrate layer 9 and the polymer packaging layer 11 is Polyimide (PI), the material of the conductive functional layer 10 is gold or platinum, and the ductile flexible electrode 2 is prepared based on a micro-nano processing technology, so that the requirements on electrode precision can be satisfied.
Referring to fig. 4, which is a schematic diagram of the relative positions of the elastic stamp 1 and the extensible flexible electrode 2 with fluid channels in an integrated state, the visual angle is in a oblique view, and the extensible flexible electrode 2 is close to the area of the electrode point, which is in direct contact with the brain, and adopts a discrete serpentine structure to improve the local extensibility; meanwhile, the artificial cerebrospinal fluid 5 can be injected into the cylindrical hole containing the fluid channel elastic seal 1 through the needle tube as required, so that the PEG can be rapidly dissolved when the extensible flexible electrode 2 contacts the target cerebral cortex 6.
In another embodiment, the materials used are replaced to achieve the same transfer effect. In the extensible flexible electrode 2, the materials of the replaced polymer substrate layer 9 and the polymer packaging layer 11 are transparent Parylene (Parylene) with good biocompatibility, the conductive functional layer 10 is replaced by a transparent conductive material compounded by conductive polymers of poly-3, 4-ethylenedioxythiophene/polystyrene sulfonate (PEDOT: PSS) and single-walled carbon nanotubes (SWCNTs), so that the transparency, conductivity, extensibility and nuclear magnetic resonance electromagnetic compatibility of the extensible flexible electrode 2 are ensured, structures such as cerebral vessels below the electrode can be observed and imaged more clearly after the extensible flexible electrode is transferred to the cerebral cortex 6, and brain electrical signals can be synchronously recorded in a nuclear magnetic resonance state after the extensible flexible electrode is implanted, and nuclear magnetic resonance imaging quality cannot be influenced. The material containing the fluid channel elastic seal 1 can be replaced by transparent thermoplastic Polyurethane (PU) with a bonding function, the water-soluble quick degradation material can be replaced by polyvinyl alcohol (PVA), and the material also has higher dissolution rate and plays a positive role in quick transfer. The specific materials can be replaced and selected according to actual needs.
In another specific embodiment, the extensible flexible electrodes 2 with different sizes and the number of electrode points are matched according to the size of a target brain region, and the matched size of the elastic seal 1 with the fluid channel is synchronously designed, for example, the size of the elastic seal 1 with the fluid channel can be designed to be 3mm multiplied by 3mm aiming at the cerebral cortex of a mouse; whereas for the cerebral cortex of non-human primates the size of the fluid channel containing elastomeric stamp 1 may be designed to be 20mm x 20mm or even larger. Simultaneously, the number and the diameter of the array type small round holes of the lower part 8 of the elastic seal can be adjusted so as to regulate and control the speed and the flow rate of the solution penetrating through the small round holes and the contact effect with the extensible flexible electrode 2.
Claims (10)
1. A method of transferring a malleable flexible electrode based on a flexible stamp having a fluid channel, comprising the steps of:
step 1: the method comprises the steps of covering an elastic seal with a fluid channel above an extensible flexible electrode, and injecting a water-soluble rapid degradation material solution into the fluid channel of the elastic seal with the fluid channel, wherein the water-soluble rapid degradation material solution is contacted with the extensible flexible electrode through a circular hole array on the bottom surface of the elastic seal with the fluid channel;
step 2: placing the elastic seal containing the fluid channel, the extensible flexible electrode and the glass substrate into an oven for heating, and hardening the water-soluble rapid degradation material solution; taking the elastic seal containing the fluid channel, the extensible flexible electrode and the glass substrate out of the oven, and then adhering the extensible flexible electrode to the bottom surface of the elastic seal containing the fluid channel through a hardened water-soluble rapid degradation material solution, wherein the extensible flexible electrode is separated from the glass substrate;
step 3: moving the fluid channel-containing elastic seal with the extensible flexible electrode adhered on the bottom surface to the upper part of a target brain region by utilizing a micro-motion platform, moving the fluid channel-containing elastic seal downwards to be in elastic contact with the cerebral cortex, and injecting artificial cerebrospinal fluid into the fluid channel containing the fluid channel-containing elastic seal to gradually dissolve the hardened water-soluble rapid degradation material solution;
step 4: the water-soluble rapid degradation material solution to be hardened is completely dissolved, and the elastic seal containing the fluid channel is lifted and separated from the extensible flexible electrode, so that the extensible flexible electrode is attached to the surface of the cerebral cortex.
2. The method of claim 1, wherein the fluid channel-containing elastomeric stamp is made of a highly transparent elastomeric material, in particular polydimethylsiloxane or polyurethane.
3. The method of claim 1, wherein the dimension of the flexible stamp is 3mm x 3mm or 20mm x 20mm.
4. The method for transferring the extensible flexible electrode based on the elastic seal with the fluid channel according to claim 1, wherein the water-soluble rapid degradation material is one of sodium alginate, polyethylene glycol, polyvinyl alcohol and sucrose.
5. The method of claim 1, wherein the ductile flexible electrode comprises a polymer substrate layer, a conductive functional layer, and a polymer encapsulation layer, the conductive functional layer being encapsulated between the polymer substrate layer and the polymer encapsulation layer; the extensible flexible electrode is close to the area where the electrode point and the brain directly contact and act, and adopts a discrete snake-shaped structure.
6. The method of claim 5, wherein the material of the polymer substrate layer and the polymer encapsulation layer is polyimide, and the material of the conductive functional layer is gold or platinum.
7. The method for transferring the extensible flexible electrode based on the elastic seal with the fluid channel according to claim 5, wherein the material of the polymer substrate layer and the polymer packaging layer is parylene, and the material of the conductive functional layer is a transparent conductive material compounded by poly 3, 4-ethylenedioxythiophene/polystyrene sulfonate and single-walled carbon nanotubes.
8. The method for transferring the extensible flexible electrode based on the elastic seal with the fluid channel according to claim 1, wherein the heating temperature in the oven in the step 2 is 70-80 ℃ and the heating time is 1-4 hours.
9. The method for transferring the extensible flexible electrode based on the elastic seal with the fluid channel according to claim 1, wherein the elastic seal with the fluid channel is divided into an upper part and a lower part, the upper part consists of a hollow cylindrical handle and a rectangular concave cavity, and the hollow part of the hollow cylindrical handle is the fluid channel; the lower part is a cuboid which can be embedded into the upper rectangular concave cavity, one surface of the cuboid is provided with a groove, the other surface is provided with a round hole array, and the round holes are through holes; and injecting liquid into the fluid channel, enabling the liquid to flow to the rectangular concave cavity along the fluid channel, and enabling the liquid to flow out of the elastic seal containing the fluid channel through the circular hole array.
10. The processing method of the elastic seal with the fluid channel is characterized by comprising the following steps of:
step 1: processing the upper part of the elastic seal containing the fluid channel by using a 3D printing resin material rapid forming die, injecting liquid PDMS into a cavity from an annular inlet at the upper end of the die by using an injector, heating in an oven at 60 ℃ for 2 hours, and taking out the upper part of the elastic seal containing the fluid channel from the die after the PDMS is not completely solidified;
step 2: the lower part of the elastic seal containing the fluid channel is processed by using a rectangular groove die with a cylindrical array formed by 3D printing resin material, liquid PDMS is poured into the die, the liquid level is not higher than the cylindrical array, the liquid level is heated in a box at 60 ℃ for 2 hours, and the lower part of the elastic seal containing the fluid channel is taken out from the die after the PDMS is not solidified completely;
step 3: and (3) activating the surfaces of the PDMS of the upper part and the lower part of the elastic seal containing the fluid channel by oxygen plasma, then immediately aligning and bonding, and putting the PDMS into an oven at 80 ℃ to heat for 2 hours, so that complete solidification is realized, and firm bonding between the two parts is ensured.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110604566A (en) * | 2019-09-24 | 2019-12-24 | 清华大学 | Flexible deformable degradable brain detection treatment device and system and manufacturing and using methods |
| WO2020233160A1 (en) * | 2019-05-17 | 2020-11-26 | 青岛理工大学 | Manufacturing method for embedded metal grid-based flexible transparent electrode and application thereof |
-
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020233160A1 (en) * | 2019-05-17 | 2020-11-26 | 青岛理工大学 | Manufacturing method for embedded metal grid-based flexible transparent electrode and application thereof |
| CN110604566A (en) * | 2019-09-24 | 2019-12-24 | 清华大学 | Flexible deformable degradable brain detection treatment device and system and manufacturing and using methods |
Non-Patent Citations (1)
| Title |
|---|
| 神经芯片研究新进展:微接触印刷将神经元和硅片融为一体;高侃;刘丙方;徐群渊;;解剖学报(第01期);全文 * |
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