CN112970974A - Processing method of aquatic animal seedling raising bait - Google Patents
Processing method of aquatic animal seedling raising bait Download PDFInfo
- Publication number
- CN112970974A CN112970974A CN202110322603.3A CN202110322603A CN112970974A CN 112970974 A CN112970974 A CN 112970974A CN 202110322603 A CN202110322603 A CN 202110322603A CN 112970974 A CN112970974 A CN 112970974A
- Authority
- CN
- China
- Prior art keywords
- bait
- processing
- material tray
- artemia
- freeze
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241001465754 Metazoa Species 0.000 title claims abstract description 16
- 238000003672 processing method Methods 0.000 title claims abstract description 16
- 239000000463 material Substances 0.000 claims abstract description 65
- 238000012545 processing Methods 0.000 claims abstract description 38
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 34
- 230000005496 eutectics Effects 0.000 claims abstract description 26
- 239000007788 liquid Substances 0.000 claims abstract description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 23
- 238000004108 freeze drying Methods 0.000 claims abstract description 22
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 17
- 238000007710 freezing Methods 0.000 claims abstract description 14
- 108010010803 Gelatin Proteins 0.000 claims abstract description 11
- 229920000159 gelatin Polymers 0.000 claims abstract description 11
- 239000008273 gelatin Substances 0.000 claims abstract description 11
- 235000019322 gelatine Nutrition 0.000 claims abstract description 11
- 235000011852 gelatine desserts Nutrition 0.000 claims abstract description 11
- 238000005507 spraying Methods 0.000 claims abstract description 9
- 238000004806 packaging method and process Methods 0.000 claims abstract description 8
- 238000011049 filling Methods 0.000 claims abstract description 7
- 238000009777 vacuum freeze-drying Methods 0.000 claims abstract description 7
- 238000001914 filtration Methods 0.000 claims abstract description 5
- 238000007789 sealing Methods 0.000 claims abstract description 5
- 241001247197 Cephalocarida Species 0.000 claims abstract 4
- 238000000034 method Methods 0.000 claims description 29
- 230000008014 freezing Effects 0.000 claims description 10
- 238000007781 pre-processing Methods 0.000 claims description 4
- 229910001220 stainless steel Inorganic materials 0.000 claims description 4
- 239000010935 stainless steel Substances 0.000 claims description 4
- 241000700141 Rotifera Species 0.000 claims description 3
- 230000008859 change Effects 0.000 claims description 3
- 230000035772 mutation Effects 0.000 claims description 3
- 238000005520 cutting process Methods 0.000 claims description 2
- 238000003754 machining Methods 0.000 claims description 2
- 238000003975 animal breeding Methods 0.000 claims 5
- 235000016709 nutrition Nutrition 0.000 abstract description 6
- 239000004615 ingredient Substances 0.000 abstract description 5
- 230000014759 maintenance of location Effects 0.000 abstract description 4
- 238000009461 vacuum packaging Methods 0.000 abstract description 3
- 238000001291 vacuum drying Methods 0.000 abstract description 2
- 241000238582 Artemia Species 0.000 description 57
- 239000000243 solution Substances 0.000 description 12
- 230000008569 process Effects 0.000 description 11
- 150000002632 lipids Chemical class 0.000 description 9
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 8
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 8
- 235000019750 Crude protein Nutrition 0.000 description 7
- 235000015097 nutrients Nutrition 0.000 description 7
- 238000004458 analytical method Methods 0.000 description 6
- WORJEOGGNQDSOE-UHFFFAOYSA-N chloroform;methanol Chemical compound OC.ClC(Cl)Cl WORJEOGGNQDSOE-UHFFFAOYSA-N 0.000 description 5
- 230000029087 digestion Effects 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 102000004169 proteins and genes Human genes 0.000 description 5
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 4
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 4
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 4
- 238000007696 Kjeldahl method Methods 0.000 description 4
- 235000014113 dietary fatty acids Nutrition 0.000 description 4
- 235000019197 fats Nutrition 0.000 description 4
- 239000000194 fatty acid Substances 0.000 description 4
- 229930195729 fatty acid Natural products 0.000 description 4
- 150000004665 fatty acids Chemical class 0.000 description 4
- 150000003904 phospholipids Chemical class 0.000 description 4
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 3
- 239000004327 boric acid Substances 0.000 description 3
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 239000012159 carrier gas Substances 0.000 description 3
- 235000020673 eicosapentaenoic acid Nutrition 0.000 description 3
- 210000002615 epidermis Anatomy 0.000 description 3
- 235000012041 food component Nutrition 0.000 description 3
- 238000000691 measurement method Methods 0.000 description 3
- 229910000069 nitrogen hydride Inorganic materials 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 241001391944 Commicarpus scandens Species 0.000 description 2
- 241000595940 Notostraca Species 0.000 description 2
- 238000000944 Soxhlet extraction Methods 0.000 description 2
- 238000005903 acid hydrolysis reaction Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000012267 brine Substances 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 229910000365 copper sulfate Inorganic materials 0.000 description 2
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 2
- 235000019784 crude fat Nutrition 0.000 description 2
- 238000000354 decomposition reaction Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 238000004817 gas chromatography Methods 0.000 description 2
- 229910052736 halogen Inorganic materials 0.000 description 2
- 150000002367 halogens Chemical class 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000011259 mixed solution Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 2
- 239000005416 organic matter Substances 0.000 description 2
- 239000003208 petroleum Substances 0.000 description 2
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 description 2
- 229910052939 potassium sulfate Inorganic materials 0.000 description 2
- 235000011151 potassium sulphates Nutrition 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 238000005057 refrigeration Methods 0.000 description 2
- 230000001932 seasonal effect Effects 0.000 description 2
- HPALAKNZSZLMCH-UHFFFAOYSA-M sodium;chloride;hydrate Chemical compound O.[Na+].[Cl-] HPALAKNZSZLMCH-UHFFFAOYSA-M 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000238426 Anostraca Species 0.000 description 1
- 241000238421 Arthropoda Species 0.000 description 1
- 241000238557 Decapoda Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 102000004895 Lipoproteins Human genes 0.000 description 1
- 108090001030 Lipoproteins Proteins 0.000 description 1
- JAZBEHYOTPTENJ-JLNKQSITSA-N all-cis-5,8,11,14,17-icosapentaenoic acid Chemical compound CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O JAZBEHYOTPTENJ-JLNKQSITSA-N 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 230000001363 autoimmune Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000007664 blowing Methods 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 238000003763 carbonization Methods 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- AWADHHRPTLLUKK-UHFFFAOYSA-N diazanium sulfuric acid sulfate Chemical compound [NH4+].[NH4+].OS(O)(=O)=O.[O-]S([O-])(=O)=O AWADHHRPTLLUKK-UHFFFAOYSA-N 0.000 description 1
- AUTNMGCKBXKHNV-UHFFFAOYSA-P diazanium;3,7-dioxido-2,4,6,8,9-pentaoxa-1,3,5,7-tetraborabicyclo[3.3.1]nonane Chemical compound [NH4+].[NH4+].O1B([O-])OB2OB([O-])OB1O2 AUTNMGCKBXKHNV-UHFFFAOYSA-P 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 229960005135 eicosapentaenoic acid Drugs 0.000 description 1
- JAZBEHYOTPTENJ-UHFFFAOYSA-N eicosapentaenoic acid Natural products CCC=CCC=CCC=CCC=CCC=CCCCC(O)=O JAZBEHYOTPTENJ-UHFFFAOYSA-N 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 210000002816 gill Anatomy 0.000 description 1
- 150000003278 haem Chemical class 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- GPRLSGONYQIRFK-UHFFFAOYSA-N hydron Chemical compound [H+] GPRLSGONYQIRFK-UHFFFAOYSA-N 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000021049 nutrient content Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000020660 omega-3 fatty acid Nutrition 0.000 description 1
- 125000001477 organic nitrogen group Chemical group 0.000 description 1
- 239000007800 oxidant agent Substances 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 238000004091 panning Methods 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 238000006479 redox reaction Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000006722 reduction reaction Methods 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 150000004671 saturated fatty acids Chemical class 0.000 description 1
- 235000015170 shellfish Nutrition 0.000 description 1
- 210000003491 skin Anatomy 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 238000000859 sublimation Methods 0.000 description 1
- 230000008022 sublimation Effects 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid Substances OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 238000002061 vacuum sublimation Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/40—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by drying or kilning; Subsequent reconstitution
- A23L3/44—Freeze-drying
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Physiology (AREA)
- Molecular Biology (AREA)
- Nutrition Science (AREA)
- Biomedical Technology (AREA)
- Insects & Arthropods (AREA)
- Marine Sciences & Fisheries (AREA)
- Birds (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses a processing method of aquatic animal seedling raising bait, which comprises the following steps: pretreatment: spraying an edible gelatin solution with the mass fraction of 10-13% to a material tray, filtering the fresh and live zooplankton baits by a screen, then filling the filtered baits into the material tray, and continuously spraying the surfaces of the baits by using the gelatin solution; liquid nitrogen quick-freezing treatment: carrying out liquid nitrogen quick-freezing processing on the material tray filled with the materials, wherein the processing time is 45-85 min, and carrying out freeze-drying treatment after the processing is finished; and (3) freeze-drying treatment: performing freeze-drying processing by using a vacuum freeze-drying machine, controlling the temperature of a vacuum bin to be lower than the eutectic point of bait during processing, controlling the air pressure of the vacuum bin to be lower than 10Pa, controlling the temperature of a cold trap to be lower than-50 ℃ until the water content of the material is lower than 1% to obtain a finished product; packaging treatment: taking out the finished product from the material tray, sealing and vacuum packaging to obtain the seedling raising bait. The retention rate of the nutritional ingredients of the treated artemia is extremely high, and the treated artemia is in a vacuum drying state and is convenient to transport and store.
Description
Technical Field
The invention belongs to the field of bait processing, and particularly relates to a processing method of aquatic animal seedling raising bait.
Background
In the stage of raising aquatic animals, artemia are generally used as feed for the raising of the seedlings. Artemia, also known as brine fairy shrimp, belongs to the genera artemia, classified in the phylum arthropoda, subhyalomycota, class gilles, order ameliota, family brine fairy shrimp. The artemia contains abundant protein, complete amino acid composition and high crude fat content, wherein unsaturated fatty acid is higher than saturated fatty acid, and the artemia is widely regarded as the best bait by the aquatic seedling culture industry. From nutrition analysis, the artemia are determined to contain rich crude protein, fat, unsaturated fatty acid and inorganic elements, wherein the 20:5 omega-3 unsaturated fatty acid (eicosapentaenoic acid, also called EPA) rich in the artemia is a kind of fatty acid necessary for marine animals, and has the effects of promoting growth and development, promoting circulatory system health and resisting autoimmune deficiency on aquatic products. Animal experiments prove that the edible artemia does not influence blood fat, and can improve the content of DHA, EPA, heme and brain protein in the liver.
Artemia, as a high-quality natural bait, is widely favored by aquaculture workers at home and abroad, but is in a state of short supply and short demand all the time because of the limitations of production places, yield and seasons. The regional and seasonal periodicity of the supply of artemia is determined by the reproductive characteristics and the growth environment of the artemia, and the artemia cannot be transported and stored on a large scale; meanwhile, the artemia are easy to break and die after leaving the living water body, so that the loss of the nutrient components in the artemia is caused, and the nutrient value is greatly reduced. In the existing process of processing artemia, the structure of the artemia is easily damaged, so that the original nutritional ingredients of the artemia are difficult to be reserved to a greater extent.
Disclosure of Invention
Aiming at the problems, the invention provides a processing method of aquatic animal seedling raising bait, which is researched and designed to solve the defects that the artemia processed by the traditional method has high nutrient loss rate and is not beneficial to transportation and storage. The technical means adopted by the invention are as follows:
a processing method of aquatic animal seedling raising bait comprises the following steps:
s1, preprocessing: spraying an edible gelatin solution with the mass fraction of 10-13% to a material tray, filtering the fresh and live zooplankton baits by a screen, then filling the filtered baits into the material tray, and continuously spraying the surfaces of the baits by using the gelatin solution;
s2, liquid nitrogen quick freezing treatment: carrying out liquid nitrogen quick-freezing processing on the material tray filled with the materials, wherein the processing time is 45-85 min, and carrying out freeze-drying treatment after the processing is finished;
s3, freeze-drying treatment: performing freeze-drying processing by using a vacuum freeze-drying machine, controlling the temperature of a vacuum bin to be lower than the eutectic point of bait during processing, controlling the air pressure of the vacuum bin to be lower than 10Pa, controlling the temperature of a cold trap to be lower than-50 ℃ until the water content of the material is lower than 1% to obtain a finished product;
s4, packaging: taking out the finished product from the material tray, and carrying out vacuum sealing packaging to obtain the seedling raising bait.
Preferably, in step S1, the zooplankton bait is artemia or rotifers.
Preferably, in step S1, the zooplankton bait is artemia.
Preferably, in step S1, the tray is a cellular tray, wherein each cell has a length and width of no more than 30mm x 15 mm.
Preferably, in step S2, the machining completion criteria are: cutting off the small bait blocks in the small grids of the material tray, and detecting the material tray without liquid water by using filter paper.
Preferably, in step S3, the eutectic point of the bait is determined by: immersing the electrode in bait, connecting the electrode into a resistance instrument, controlling the temperature of the bait to gradually decrease to-50 ℃, and simultaneously recording the change condition of the resistance value, wherein the mutation point of the resistance value is the eutectic point and the eutectic point of the bait, the point with higher temperature is the eutectic point, and the point with lower temperature is the eutectic point.
Preferably, the material of the material tray is 304 food-grade stainless steel.
Compared with the prior art, the processing method of the aquatic animal seedling raising bait has the beneficial effects that: the invention provides a method for processing artemia, and the bait product processed by the method has the characteristic of extremely high retention rate of nutritional ingredients of the artemia. Compared with fresh and alive artemia, the dry artemia processed by the method has the advantages that the loss rate of crude protein is 11.8 percent, the loss rate of total fat content is 0.48 percent, the loss rate of unsaturated fatty acid is 69.4 percent and the loss of nutritional ingredients is extremely low after the dry artemia is soaked in water. The loss rate of the nutrient components of the brine shrimp after common freezing is very high, the loss rate of crude protein and fat exceeds 30 percent, and the unsaturated fatty acid can not detect residues basically. Meanwhile, the processed dry artemia are in a vacuum drying state, so that the dry artemia are convenient to store and transport, the limit that the live artemia are used as bait is eliminated, the problem that fresh and alive artemia are not available for feeding shrimp seeds in autumn and winter due to the seasonal breeding of the artemia is solved, and the problem that the artemia cannot be fed due to the fact that no water body suitable for breeding of the artemia exists in the places of the southern aquatic product breeding enterprises is solved.
Detailed Description
A processing method of aquatic animal seedling raising bait comprises the following steps:
s1, preprocessing: spraying edible gelatin solution with the mass fraction of 10-13% on the inner wall of a material tray before filling materials, filtering fresh and live zooplankton bait through a screen, filling the filtered fresh and live zooplankton bait into the material tray, and spraying the edible gelatin solution with the mass fraction of 10-13% on the surface of the bait;
s2, liquid nitrogen quick freezing treatment: putting the material tray filled with the materials into a liquid nitrogen instant freezer for quick freezing processing, wherein the processing time is 45-85 min, and transporting the material tray to a freeze-drying treatment place through refrigeration and transportation after the processing is finished;
s3, freeze-drying treatment: performing freeze-drying processing by using a vacuum freeze-drying machine, wherein the temperature of a vacuum chamber is controlled to be lower than the eutectic point of bait during processing, the air pressure of the vacuum chamber is lower than 10Pa, the temperature of a cold trap is lower than-50 ℃, and the water capturing capacity of the freeze-drying machine is that the kilogram number of captured water per 24 hours is not lower than 50 Kg;
the processing time is determined according to the power of the freeze dryer, and the processed finished product has the standard that the water content of the material measured by a halogen moisture meter is lower than 1 percent;
s4, packaging: taking out the finished product from the material tray, sealing and vacuum packaging to obtain the seedling raising bait.
The bait processed by the method comprises zooplankton including artemia, rotifer and the like, and is mainly applied to the production stage of various aquatic animal seedlings.
In step S1, the tray is made of 304 food grade stainless steel, and the length, width and height of each small cell of the tray are not more than 30mm 15 mm. Under this size, the material reaches more balanced effect in freeze-drying time and freeze-drying effect, and the size too big can lead to freeze-drying time extension and drying effect not good, and the size too little leads to the finished product breakable to increased the blowing and got the time of step such as material, it is long consuming time.
In step S2, the processing standard requires that the center of the material piece in each cell of the material tray be thoroughly frozen, and the center of the material piece is cut open to detect the absence of water in liquid form with experimental filter paper.
In step S3, the method for determining the eutectic point of the bait includes: the method is characterized in that a resistance method is adopted for testing, the principle that materials have different resistivity under different forms is utilized, in the actual measurement process, an electrode is placed in a beaker filled with material liquid, two ends of the electrode are connected into a resistance instrument, a thermometer is inserted into the materials, the beaker is placed into a refrigerator, the temperature is slowly reduced to-50 ℃, a computer records the change condition of the resistance value, the mutation point of the resistance value is the eutectic point and the eutectic point of the materials, wherein the point with higher temperature is the eutectic point, and the point with lower temperature is the eutectic point. The eutectic point and the eutectic point are two important parameters of the material in vacuum freeze drying, below the eutectic point, the material is completely converted into a solid state, and above the eutectic point, the material begins to be converted from the solid state into a liquid state. Since the principle of the freeze-drying process is sublimation, the intermediate liquid process is skipped, and thus the temperature of the vacuum chamber where the material is located in step S3 must be lower than the eutectic point, otherwise the material will melt and fail to achieve the effect of vacuum freeze-drying.
Once the artemia are separated from the living water body, the skin of the artemia is easy to break in a short time, so that the loss of nutrient components is caused. In the process of processing by adopting the traditional freezing method, firstly, the time required by freezing is longer, so that the epidermis of the worm body is naturally cracked, secondly, the water in the artemia body is frozen and expanded along with the reduction of the temperature in the freezing process, so that the epidermis of the worm body is cracked, and the nutrient content is further lost in the unfreezing process.
When the method is used for processing, as liquid nitrogen is used for rapid cooling, the required time is short, the processing time of the living artemia is reduced, and the probability of the rupture of the epidermis of the artemia is greatly reduced; meanwhile, in the freeze drying process, moisture in the worm body is removed through vacuum sublimation, and the thawing process does not exist, so that the loss rate of nutrient components can be greatly reduced.
The specific embodiment is as follows:
a processing method of aquatic animal seedling raising bait comprises the following steps:
s1, preprocessing: a material tray is prepared. The material tray is a grid tray made of 304 food-grade stainless steel, the size of the material tray is determined according to the size of a liquid nitrogen instant freezer, the length, the width and the height of each small grid of the material tray are required to be not more than 30mm, 30mm and 15mm, before the materials are filled, 12% concentration edible gelatin solution is used for spraying the bottom surface and the side surface of the material tray for a short time, a layer of gelatin solution film is formed on the surface of the material tray, and the dried materials are easy to form; filtering the salvaged fresh artemia by using a screen to remove impurities, quickly filling the sieved artemia into a material tray, and flattening the surface of the artemia and ensuring that the surface of the artemia is even and the thickness of the artemia is uniform after filling, so that the artemia is prevented from being cracked due to extrusion; after the panning was completed, the surface was sprayed again with a 12% strength edible gelatin solution for a short time.
S2, liquid nitrogen quick freezing treatment: the loaded material tray is placed into a liquid nitrogen instant freezer to be subjected to quick freezing processing, the center of a small material block in each small cell of the material tray is completely frozen according to the processing standard, the color of the small material block is wholly changed into pink (the color displayed after artemia are dehydrated), and the center of the small material block is cut open to detect water which cannot exist in a liquid form by using experimental filter paper. The weight of the processed materials is 2000g, the processing time is determined to be 65min, and the processed materials are transported to a freeze-drying treatment place through refrigeration transportation after being processed;
s3, freeze-drying treatment: the freeze-drying processing is carried out by using a vacuum freeze-drying machine, the temperature of a vacuum chamber is required to be controlled to be lower than the eutectic point of artemia when processing is carried out, the eutectic point of a tested sample is about-14.3 to-12.9 ℃ because of the difference of different artemia individuals, the temperature of the vacuum chamber is controlled to be about-15 ℃, the air pressure of the vacuum chamber is 0.1Pa, the temperature of a cold trap is-50 ℃, and the water catching capacity of the freeze-drying machine is 50Kg/24 h. The processing time is determined according to the power of the freeze dryer, and the processed finished product has the standard that the water content of the material measured by a halogen moisture meter is lower than 1 percent.
S4, packaging: and after the processing is finished, taking out the finished products from the material tray, arranging the finished products, putting the finished products into a sealed packaging bag for vacuum packaging, and finally boxing.
Measuring the nutrient component retention rate of the obtained bait finished product:
1. adopting a single variable method principle, taking two fresh and live adult artemia with the same weight, directly analyzing the nutritional components of one adult artemia, processing the other adult artemia by the method of the invention, immersing the dried artemia into the water body in which the artemia are caught, and analyzing the nutritional components after the dried artemia is completely immersed.
2. The analysis of nutritional components mainly comprises analysis of three components, namely crude protein content analysis, total fat content analysis and fatty acid component analysis.
1) Crude protein measurement method: kjeldahl method
The Kjeldahl method determines the protein content by measuring the total nitrogen content in the sample and multiplying by the corresponding protein coefficient, the result of which is called the crude protein content. The Kjeldahl method firstly heats the artemia bait finished product (nitrogenous organic matter) and concentrated sulfuric acid together, and converts organic nitrogen into inorganic nitrogen ammonium sulfate through a series of complex processes such as decomposition, carbonization, oxidation reduction reaction and the like, wherein the process is called as the digestion of the organic matter. In order to accelerate and completely decompose organic substances and shorten digestion time, reagents such as potassium sulfate, copper sulfate, hydrogen peroxide and the like are added during digestion, the boiling point of digestion liquid can be increased by adding the potassium sulfate so as to accelerate the decomposition of the organic substances, the copper sulfate plays a role of a catalyst, and the oxidation of the organic substances can be accelerated by adding a small amount of hydrogen peroxide as an oxidizing agent. After digestion, transferring the digestive juice into a Kjeldahl nitrogen determination instrument reaction chamber, adding excessive concentrated sodium hydroxide, and adding NH4 +Conversion to NH3By distillation of NH3Driving the solution into an excessive boric acid receiving bottle, forming ammonium tetraborate after the boric acid receives ammonia, and then titrating with standard hydrochloric acid until the original hydrogen ion concentration of the boric acid solution is restored. The mole number of the standard hydrochloric acid consumed by titration is NH3The total nitrogen content can be obtained by calculation. Because proteins are a complex class of nitrogen-containing compounds, each protein has its constant nitrogen content,and multiplying the nitrogen content determined by the Kjeldahl method by a coefficient of 6.25 to obtain the protein content.
2) Method for measuring total fat content: chloroform-methanol solution extraction method
Common animal and plant fat measurement methods include Soxhlet extraction, acid hydrolysis and chloroform-methanol solution extraction. The Soxhlet extraction method can only extract free fat, but can not completely extract combined lipids such as lipoprotein, phospholipid and the like; the acid hydrolysis method can lead the phospholipid to be hydrolyzed and lost; in the presence of certain water content, the polar methanol and non-polar chloroform mixed solution can effectively extract the combined lipid, is suitable for samples with higher combined lipid content, especially high phospholipid content, such as fresh fish, shellfish meat, poultry eggs and the like, and is more effective for samples with high water content.
Specifically, the artemia bait sample is dispersed in chloroform-methanol mixed solution and slightly boiled in water bath, chloroform-methanol and water contained in the sample form a solvent for extracting lipid, so that the affinity of the chloroform-methanol and polar lipids such as phospholipid is increased while bound lipids in sample tissues are dissociated, all the lipids are effectively extracted, non-lipid components are filtered out, the solvent is recovered, the residual lipids are extracted by petroleum ether, and the quantity is determined after the petroleum ether is evaporated.
3) Fatty acid measurement method: gas chromatography method
The gas chromatography is characterized in that a carrier and stationary liquid are loaded into a chromatographic column, a carrier gas is used for carrying an analyzed mixture into the chromatographic column, distribution coefficients of all gas components in gas-liquid two-phase phases of the carrier gas and a stationary liquid film are different under certain temperature and pressure conditions, and the components of a sample are repeatedly distributed in the gas-liquid two-phase phases along with forward flowing of the carrier gas, so that the moving speed of all components of fatty acid is high or low, and the components can be separated and then are respectively measured.
3. And comparing and analyzing the two groups of experimental data, and calculating the retention rate of the nutritional ingredients of the artemia after processing. The loss rate of crude protein, the loss rate of total fat content and the loss rate of unsaturated fatty acid of the processed dry artemia after soaking through water are respectively 11.8%, 0.48% and 69.4%. Wherein, because unsaturated fatty acid is easy to be oxidized and decomposed, the loss of the unsaturated fatty acid with the ratio of 20:5 omega 3 is relatively large, and the loss rate can be reduced by controlling vacuum sealing and low-temperature storage after processing in the actual process.
The above-mentioned embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solution of the present invention by those skilled in the art should fall within the protection scope defined by the claims of the present invention without departing from the spirit of the present invention.
Claims (7)
1. A processing method of aquatic animal seedling raising bait is characterized in that: the method comprises the following steps:
s1, preprocessing: spraying an edible gelatin solution with the mass fraction of 10-13% to a material tray, filtering the fresh and live zooplankton baits by a screen, then filling the filtered baits into the material tray, and continuously spraying the surfaces of the baits by using the gelatin solution;
s2, liquid nitrogen quick freezing treatment: carrying out liquid nitrogen quick-freezing processing on the material tray filled with the materials, wherein the processing time is 45-85 min, and carrying out freeze-drying treatment after the processing is finished;
s3, freeze-drying treatment: performing freeze-drying processing by using a vacuum freeze-drying machine, controlling the temperature of a vacuum bin to be lower than the eutectic point of bait during processing, controlling the air pressure of the vacuum bin to be lower than 10Pa, controlling the temperature of a cold trap to be lower than-50 ℃ until the water content of the material is lower than 1% to obtain a finished product;
s4, packaging: taking out the finished product from the material tray, and carrying out vacuum sealing packaging to obtain the seedling raising bait.
2. The processing method of an aquatic animal breeding bait according to claim 1, characterized in that: in step S1, the zooplankton bait is artemia or rotifers.
3. The processing method of aquatic animal breeding bait according to claim 2, characterized in that: in step S1, the zooplankton bait is artemia.
4. The processing method of an aquatic animal breeding bait according to claim 1, characterized in that: in step S1, the tray is a grid tray, wherein each grid has a length, width and height of no more than 30mm, 30mm and 15 mm.
5. The processing method of an aquatic animal raising bait according to claim 4, characterized in that: in step S2, the machining completion criteria are: cutting off the small bait blocks in the small grids of the material tray, and detecting the material tray without liquid water by using filter paper.
6. The processing method of an aquatic animal breeding bait according to claim 1, characterized in that: in step S3, the method for determining the eutectic point of the bait includes: immersing the electrode in bait, connecting the electrode into a resistance instrument, controlling the temperature of the bait to gradually decrease to-50 ℃, and simultaneously recording the change condition of the resistance value, wherein the mutation point of the resistance value is the eutectic point and the eutectic point of the bait, the point with higher temperature is the eutectic point, and the point with lower temperature is the eutectic point.
7. The processing method of an aquatic animal breeding bait according to claim 1, characterized in that: the material tray is made of 304 food-grade stainless steel.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202110322603.3A CN112970974A (en) | 2021-03-25 | 2021-03-25 | Processing method of aquatic animal seedling raising bait |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202110322603.3A CN112970974A (en) | 2021-03-25 | 2021-03-25 | Processing method of aquatic animal seedling raising bait |
Publications (1)
Publication Number | Publication Date |
---|---|
CN112970974A true CN112970974A (en) | 2021-06-18 |
Family
ID=76334548
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202110322603.3A Pending CN112970974A (en) | 2021-03-25 | 2021-03-25 | Processing method of aquatic animal seedling raising bait |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN112970974A (en) |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103340329A (en) * | 2013-07-25 | 2013-10-09 | 浙江康惠生物科技有限公司 | Aquatic seedling bait and preparation process thereof |
CN112293606A (en) * | 2020-10-12 | 2021-02-02 | 上海海洋大学 | Method for preparing high-value aquatic bait by using microalgae and artemia |
-
2021
- 2021-03-25 CN CN202110322603.3A patent/CN112970974A/en active Pending
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103340329A (en) * | 2013-07-25 | 2013-10-09 | 浙江康惠生物科技有限公司 | Aquatic seedling bait and preparation process thereof |
CN112293606A (en) * | 2020-10-12 | 2021-02-02 | 上海海洋大学 | Method for preparing high-value aquatic bait by using microalgae and artemia |
Non-Patent Citations (1)
Title |
---|
隋丽英,MARIJKE VAN SPEBROECK,PATRICK SORGELOOS: "卤虫成虫及脱壳卵在水产育苗中的应用", 海湖盐与化工 * |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Senso et al. | On the possible effects of harvesting season and chilled storage on the fatty acid profile of the fillet of farmed gilthead sea bream (Sparus aurata) | |
Jakhar et al. | Fatty acids composition of some selected Indian fishes | |
Rouvinen et al. | Influence of dietary fat source on the body fat composition of mink (Mustela vison) and blue fox (Alopex lagopus) | |
Yimer et al. | Comparison of cryopreservative effect of different levels of omega-3 egg-yolk in citrate extender on the quality of goat spermatozoa. | |
Ikauniece et al. | Evaluation of nutrition value of Roman snail’s (Helix pomatia) meat obtained in Latvia | |
CN107258886A (en) | A kind of method for improving the mackerel stored frozen phase | |
Głogowski et al. | Fatty acid profile of hind leg muscle in female and male nutria (Myocastor coypus Mol.), fed green forage diet | |
KR101883616B1 (en) | Manufacturing method of processed fishery products for cryopreserving | |
Krzeczkowski | Fatty acids in raw and processed Alaska pink shrimp | |
Yao et al. | Effect of Feeing Frequency on Lipid Accumulation in Ayu | |
CN112970974A (en) | Processing method of aquatic animal seedling raising bait | |
Suárez et al. | Discarded fish on the Spanish Mediterranean coast: influence of season on fatty acids profiles | |
JP6496972B2 (en) | Oil derived from sturgeon egg sac membrane | |
CN110367192B (en) | Method for improving blood health index and meat quality of fattening pig | |
Ayeloja et al. | Effect of storage time on the quality of smoked heteroclarias | |
KR100826868B1 (en) | A method for preparing brown croaker salted using a deep ocean water salt | |
Zaharia et al. | Changes in fatty acid composition and cholesterol content of goat colostrum | |
Umar et al. | Nutritive Value of Fresh and Smoked fish Clarias gariepinus and Oreochromis nilotic us) from Dadin Kowa Dam Gombe | |
JPS6055096A (en) | Manufacture of neutral lipid from fishes and shells | |
Molina G et al. | Cellulolytic and Butyrivibrio fibrisolvens bacteria population density, after supplementing fodder diets (Pennisetum clandestinum) | |
Jørgensen | Carbon and nitrogen utilization in developing eggs and larvae of cod (Gadus morhua, L.) and variation among different parents | |
Indarto et al. | In-vivo Test of Calcium Soap from Palm Fatty Acid Distillate (PFAD) in Three Cow Farms | |
CN107996942A (en) | A kind of fairy shrimp nauplius preserving agent and its method for preserving | |
Norollahi et al. | Determination of chemical composition and digestibility of almond hull | |
Suseno et al. | Effect of solvent ratio on omega-3 extraction of catfish (Clarias sp.) and pangasius catfish (Pangasius sp.) using low-temperature crystallization method |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
RJ01 | Rejection of invention patent application after publication | ||
RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210618 |