CN112961565B - Tantalum-reinforced polyether-ether-ketone composite coating and preparation method and application thereof - Google Patents
Tantalum-reinforced polyether-ether-ketone composite coating and preparation method and application thereof Download PDFInfo
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Abstract
The invention belongs to the technical field of biological materials, and particularly discloses a tantalum-reinforced polyether-ether-ketone composite coating and a preparation method and application thereof. The method comprises the following steps: (1) uniformly mixing a chitosan solution and ethanol, sequentially adding PEEK powder and nano tantalum to obtain a mixed solution, and performing ultrasonic dispersion to obtain a nano suspension; (2) putting cathode and anode electrode materials into the nano suspension solution obtained in the step (1); and introducing direct current, and depositing to obtain the tantalum-reinforced polyether-ether-ketone composite coating. The invention prepares PEEK coating on titanium and its alloy, stainless steel and other basal bodies by a cathodic electrodeposition method, and introduces tantalum nano particles into PEEK material. The introduction of tantalum nanoparticles can enhance the osteogenic properties of PEEK. The coating prepared by cathodic electrodeposition has short production time, simple equipment, low price and wide application prospect.
Description
Technical Field
The invention belongs to the technical field of biological materials, and particularly relates to a tantalum-reinforced polyether-ether-ketone composite coating and a preparation method and application thereof.
Background
Biomedical bone implant materials have been widely used in the field of functional repair of human bodies such as joint replacement. The modulus of elasticity of traditional orthopedic alloys and ceramics is 10 to 20 times greater than that of natural bone, and the unmatched modulus of elasticity will produce a stress shielding effect, resulting in aseptic loosening of the implant and bone loss. The modulus of polymers is particularly low compared to metals. At present, polymers are widely used in various biomedical fields, and Polyetheretherketone (PEEK) is one of them.
PEEK has more excellent biocompatibility, can not generate immune reaction with bone tissues or even other tissues; also, PEEK has good chemical resistance, which avoids degradation of the mechanical properties of the metal implant due to corrosion. PEEK, however, is generally biologically inert, thereby preventing osteointegration in the body after implantation.
There is therefore a need to improve the mechanical properties and biological activity of PEEk, thereby providing a potential new composite biomaterial for orthopedic applications.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention mainly aims to provide a preparation method of a tantalum-reinforced polyetheretherketone composite coating.
The invention also aims to provide the tantalum-reinforced polyether-ether-ketone composite coating prepared by the method.
The invention further aims to provide application of the tantalum-reinforced polyether-ether-ketone composite coating in preparation of bone materials.
The purpose of the invention is realized by the following scheme:
a preparation method of a tantalum reinforced polyether-ether-ketone composite coating comprises the following steps:
(1) uniformly mixing a chitosan solution and ethanol, sequentially adding PEEK and nano tantalum to obtain a mixed solution, and performing ultrasonic dispersion to obtain a nano suspension;
(2) putting cathode and anode electrode materials into the nano suspension solution obtained in the step (1); and introducing direct current, and depositing to obtain the tantalum-reinforced polyether-ether-ketone composite coating.
Step (1), ethanol: the volume ratio of the chitosan solution is 1-2: 2-1;
the concentration of the chitosan solution in the step (1) is 0.5-0.15 wt%.
In the mixed solution in the step (1), the mass-volume ratio of PEEK, nano tantalum and chitosan solution is 1-5 g: 0.01-0.2 g: 50 mL.
The cathode material in the step (2) is pure titanium; preferably, the cathode material is cleaned before use, specifically, the surface is sand-blasted to form a rough surface, and the rough surface is cleaned by absolute alcohol. The anode material is a common inert electrode, and is preferably graphite or platinum sheet.
In the step (2)The voltage of the direct current is 10-30V; the current density is 0.01-0.5A/cm2Preferably 0.16A/cm2. The distance between the electrodes is 1-3 cm; the deposition time is 1-5 min.
The tantalum-reinforced polyether-ether-ketone composite coating is prepared by the method.
The tantalum-reinforced polyether-ether-ketone composite coating is applied to the preparation of an osteogenic material.
Compared with the prior art, the invention has the following advantages and beneficial effects:
the invention prepares PEEK coating on the titanium substrate by a cathode electrodeposition method, and introduces tantalum nano particles into PEEK material. The introduction of tantalum nanoparticles can enhance the osteogenic properties of PEEK. The coating prepared by cathodic electrodeposition has short production time, simple equipment, low price and wide application prospect.
Drawings
FIG. 1 is a chart of cell proliferation potentials on the surfaces of the materials of examples 1 and 11.
FIG. 2 is a quantitative analysis of ALP activity in 4-day and 7-day osteoinduction in examples 1 and 11.
FIG. 3 is a quantitative analysis of alizarin red staining after 7 days of osteogenic induction in examples 1 and 11.
Detailed Description
The present invention will be described in further detail with reference to examples and drawings, but the embodiments of the present invention are not limited thereto.
The reagents used in the examples are commercially available without specific reference.
The tantalum reinforced polyether-ether-ketone composite coating in the embodiments 1 to 11 is prepared by the following method:
(1) chitosan solution: mixing 0.5g of chitosan, 5mL of glacial acetic acid and 500mL of deionized water;
(2) uniformly mixing the 50mL of chitosan solution with 50mL of ethanol, and then adding 1-5 g of PEEK powder and 0.01-0.2 g of nano tantalum to obtain a mixed solution;
(3) and (3) ultrasonically dispersing the mixed solution obtained in the step (2) for 20min, and stirring and dissolving for 7h to obtain a nano suspension solution.
(4) Cathode (pure titanium) and anode (Pt) electrode materials are put into the nano suspension solution obtained in the step (3), and the electrode distance is 1-2 cm; introducing direct current, applying voltage of 30V and current density of 0.01-0.5A/cm2And depositing for 1-5min to obtain the tantalum-reinforced polyether-ether-ketone composite coating.
TABLE 1 Condition parameters of examples 1-11 and properties of the coatings obtained
First, evaluation of cell compatibility
In order to evaluate the cell proliferation capacity of the tantalum-reinforced polyetheretherketone composite coating, the materials obtained in examples 1 and 11 and pure titanium were subjected to cell proliferation by a CCK-8 kit. The experimental process is as follows:
(1) inoculating mouse osteoblast MC3T3-E1 on the autoclaved sample, and sampling for 1 day, 3 days and 7 days respectively;
(2) adding a CCK-8 solution (the adding ratio is 1:10) into the sample at the time node, incubating for 2h at 37 ℃ in the dark, and collecting the supernatant;
(3) after incubation, transfer 100 μ L of solution from each well to a 96-well plate;
(4) and detecting the absorbance of the solution by a microplate reader, wherein the detection wavelength is 450 nm.
As can be seen in fig. 1, the survival rate of cells on the material gradually increases along with the increase of time, the cell activity of MC3T3-E1 is good, the tantalum-reinforced polyetheretherketone coating layer shows more excellent cell compatibility, and the cell growth rate is 89.8%, 97.5% and 102.4% on the first day, the third day and the seventh day.
Second, osteogenic differentiation evaluation
In order to evaluate the influence of the tantalum-reinforced polyetheretherketone composite coating on the late differentiation of cells, the in vitro osteogenic differentiation of MC3T3-E1 was analyzed by alizarin red staining on the materials obtained in examples 1 and 11 and pure titanium. The experimental process is as follows:
(one) osteogenic Induction
(1) Inoculating MC3T3-E1 to the autoclaved sample;
(2) removing culture solution after 24h, and replacing with bone induction culture solution (containing 50mg/L ascorbic acid, 108 μ M dexamethasone and 10M M glycerol phosphate);
(3) then, the solution is changed every 48 h.
Quantitative analysis of Activity of (II) ALP enzyme
(1) After inducing for 4 and 7 days, respectively, removing osteogenesis inducing culture solution, and washing with PBS for 3 times;
(2) adding RIPA lysis buffer solution containing 1mM protein protection solution PMSF to extract protein;
(3) centrifuging for 5 min;
(4) taking 50 mu L of the supernatant fluid to be put into a 96-well plate, adding 50 mu L of ALP chromogenic substrate, uniformly mixing, incubating for 30min at 37 ℃, and detecting the light absorption value at the wavelength of 405nm by an enzyme-labeling instrument;
(5) taking 20 mu L of supernatant liquid to be put into a 96-well plate, adding 200 mu L of BCA working solution, uniformly mixing, incubating for 30min at 37 ℃, and detecting the light absorption value at the position of 570nm wavelength by an enzyme-labeling instrument;
(III) alizarin red staining quantitative analysis, which comprises the following steps:
(1) after induction culture for 7 days, removing osteogenic induction culture solution, and washing with PBS for 3 times;
(2) fixing with 4% paraformaldehyde solution for 30min, and washing with PBS for 3 times;
(3) adding alizarin red S solution, dyeing at room temperature for 3-5min, and washing with PBS for 3 times;
(4) on the basis of the staining, 5% perchloric acid was added, and then the absorbance was measured at 450nm using a microplate reader.
The ALP activity quantitative detection and alizarin red staining quantitative analysis show that the ALP expression and calcium nodule formation of the coating prepared in example 1 are obviously higher than that of PEEK coating and uncoated pure titanium (shown in figures 2 and 3). The results demonstrate that the tantalum reinforced polyetheretherketone composite coating has higher bioactivity and osteogenic response than PEEK coating and uncoated pure titanium.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Claims (8)
1. A preparation method of a tantalum-reinforced polyether-ether-ketone composite coating is characterized by comprising the following steps:
(1) uniformly mixing a chitosan solution and ethanol, sequentially adding PEEK powder and nano tantalum to obtain a mixed solution, and performing ultrasonic dispersion to obtain a nano suspension;
(2) putting cathode and anode electrode materials into the nano suspension obtained in the step (1); introducing direct current, and depositing to obtain the tantalum-reinforced polyether-ether-ketone composite coating;
in the mixed solution in the step (1), the mass-volume ratio of the PEEK powder to the nano tantalum to the chitosan solution is 3g:0.1 g: 50 mL;
the current density of the direct current in the step (2) is 0.16A/cm2The deposition time was 2 min.
2. The method of claim 1, wherein: the chitosan solution in step (1) is prepared by mixing 0.5g of chitosan, 5mL of glacial acetic acid and 500mL of deionized water.
3. The method of claim 1, wherein: step (1), ethanol: the volume ratio of the chitosan solution is 1: 1.
4. the method of claim 1, wherein: the voltage of the direct current in the step (2) is 30V.
5. The method of claim 1, wherein: the distance between the electrodes in the step (2) is 1-2 cm.
6. The method of claim 1, wherein: the cathode electrode in the step (2) is made of pure titanium; the anode electrode is made of a commonly used inert electrode.
7. A tantalum-reinforced polyether-ether-ketone composite coating prepared by the method of any one of claims 1 to 6.
8. Use of the tantalum-reinforced polyetheretherketone composite coating of claim 7 in the preparation of an osteogenic material.
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060099456A1 (en) * | 2000-12-28 | 2006-05-11 | Redepenning Jody G | Electrolytic deposition of coatings for prosthetic metals and alloys |
| CN103614699A (en) * | 2013-12-16 | 2014-03-05 | 中国科学院上海硅酸盐研究所 | Method for modifying surface of polyetheretherketone by injecting tantalum ion and modified polyetheretherketone material |
| WO2014186802A1 (en) * | 2013-05-17 | 2014-11-20 | Biotectix, LLC | Impregnation of a non-conductive material with an intrinsically conductive polymer |
| CN104203293A (en) * | 2012-02-07 | 2014-12-10 | 加利福尼亚大学董事会 | Products of manufacture having tantalum coated nanostructures, and methods of making and using them |
| CN106011974A (en) * | 2016-06-22 | 2016-10-12 | 中国船舶重工集团公司第七二五研究所 | Electrophoretic deposition preparation method for polyether-ether-ketone coating |
| CN110935069A (en) * | 2018-09-25 | 2020-03-31 | 华东理工大学 | Composite material, raw material composition, bone restoration body, preparation method and application |
-
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Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060099456A1 (en) * | 2000-12-28 | 2006-05-11 | Redepenning Jody G | Electrolytic deposition of coatings for prosthetic metals and alloys |
| CN104203293A (en) * | 2012-02-07 | 2014-12-10 | 加利福尼亚大学董事会 | Products of manufacture having tantalum coated nanostructures, and methods of making and using them |
| WO2014186802A1 (en) * | 2013-05-17 | 2014-11-20 | Biotectix, LLC | Impregnation of a non-conductive material with an intrinsically conductive polymer |
| CN103614699A (en) * | 2013-12-16 | 2014-03-05 | 中国科学院上海硅酸盐研究所 | Method for modifying surface of polyetheretherketone by injecting tantalum ion and modified polyetheretherketone material |
| CN106011974A (en) * | 2016-06-22 | 2016-10-12 | 中国船舶重工集团公司第七二五研究所 | Electrophoretic deposition preparation method for polyether-ether-ketone coating |
| CN110935069A (en) * | 2018-09-25 | 2020-03-31 | 华东理工大学 | Composite material, raw material composition, bone restoration body, preparation method and application |
Non-Patent Citations (2)
| Title |
|---|
| Electrophoretic deposition and microstructure development of Si3N4/polyetheretherketone coatings on titanium alloy;Tomasz Moskalewicz等;《Surface & Coatings Technology》;20180925;第350卷;正文第2.2-2.3节，第3.3节最后1段，图4、5 * |
| 钽金属促成骨特性在骨内植体材料中的应用进展;陆萌萌等;《现代医学》;20190331;第47卷(第3期);全文 * |
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