CN112946724B - Neutron radiation dose detection method based on needle-leaved pea dry seeds - Google Patents
Neutron radiation dose detection method based on needle-leaved pea dry seeds Download PDFInfo
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Abstract
The invention discloses a neutron radiation dose detection method based on dry needle pea seeds, which comprises a placing and sampling method of a neutron detection carrier needle pea seed, an experimental acquisition method of key measurement parameters SEI and StEI, and a calculation method of neutron absorption dose. The detection carrier used by the invention is needle pea seeds, and compared with the traditional detection method using a physical detector, the detection carrier has the advantages that the price is only one hundred thousand, even one millionth, and the cost is low. The needle pea seeds belong to green organisms, and compared with a physical detector belonging to an electrical device, the needle pea seeds are more green and environment-friendly in subsequent recovery treatment after use. In addition, the needle pea seeds have strong resistance, are easy to obtain, do not need special management, and have the effects of reducing management cost and purchasing cost and being convenient to use. Compared with the traditional detection method using nuclear electronics devices, the greenhouse seedling culture experimental method adopted by the invention is easier to learn and master and is convenient to popularize.
Description
Technical Field
The invention belongs to the technical field of nuclear science, and particularly relates to a neutron radiation dose detection method based on needle-leaved pea dry seeds.
Background
The nuclear radiation is photon flux, ray, particle or nuclear fission product, etc., and can react with organism. When an organism is exposed to ionizing radiation, nuclear radiation can have various effects on the organism at the molecular, cellular, tissue or organ level, resulting in complex changes in the organism, including physical, chemical and biological aspects. In the process of nuclear radiation biological research and practical application, the nuclear radiation dose has very important influence on the radiation biological effect and mutagenesis of organisms. Generally, when the nuclear radiation dose reaches a certain value, biological cells are obviously damaged, and the damage degree is larger along with the increase of the dose, especially the nuclear radiation with medium and high dose has great harmfulness, so that the cells cannot be repaired in time to generate mutation (canceration), and even the organism can die.
Neutrons were bombarded by alpha particles by Chadwich et al in 1932 9 Be and 10 b, experimental study. It is an important component of the nuclear structure, present in all nuclei except hydrogen. Neutrons are uncharged, and compared with traditional photon radiation (x-rays, gamma rays and microwave radiation), neutron radiation has the characteristics of strong penetration capacity, wide variation spectrum, high variation rate, relatively stable properties of variant offspring and the like, so that a more obvious and higher radiation biological effect can be generated, and the neutron radiation is a common type in nuclear radiation.
Devices that generally produce neutron radiation: nuclear power plant reactors, some accelerator devices, and isotope neutron sources installed in neutron imaging devices. The use of these devices inevitably requires the detection of the neutron radiation cumulative dose, such as the detection of the neutron radiation dose in different installation areas of the device, the detection of the radiation dose after the neutron radiation leakage of the device, and the like, so as to evaluate the safety of the device to operators and the environment. The traditional neutron radiation dose detection is completed by utilizing various physical detectors and nuclear electronic devices, and because neutron radiation has a single event effect on the physical detectors, the physical detectors are mostly used for short-time detection of neutron radiation dose of the device; in addition, the physical detector and the nuclear electronic device have poor resistance, special acquisition channels, special management and high cost, and are not beneficial to popularization and application.
An annual or perennial herb of pea (Pisum sativum L.) of the family Leguminosae (Fabaceae) Papilionaceae (Faboideae) of the genus Pisum (Pisum). The plant is not only a grain crop with high economic value, but also a model plant for classical genetic research, has various morphological characters which are easy to identify, and is widely applied to various levels of plant biology research. The needle-leaved peas are an important line of peas and mainly show that the main leaves are needle-leaved, the leaves are large and mainly perform photosynthesis. The applicant finds that the neutron irradiation needle pea dry seeds have obvious dose effect in the research of the biological effect of fission neutron irradiation pea seeds, and a method for detecting the neutron irradiation dose can be constructed according to the dose effect.
Disclosure of Invention
The invention aims to provide the neutron radiation dose detection method based on the dry needle-leaved pea seeds, which is low in cost, green and environment-friendly.
The purpose of the invention is realized by the following technical scheme:
a neutron radiation dose detection method based on needle pea dry seeds comprises the following steps:
A. selecting dry needle pea seeds with plump seeds and a germination rate of more than 95%, and placing the seeds in a neutron radiation dose detection area;
B. when neutron radiation dose detection is needed, randomly extracting at least 3 seeds subjected to neutron irradiation in the step A; the invention measures the cumulative absorbed dose of pea seeds, and other pea seeds can be accumulated continuously for subsequent measurement due to partial sampling. Therefore, the invention can freely design a plurality of sampling modes according to the actual requirements.
C. Taking the same number of dry seeds which are free from irradiation as the seeds to be detected as control seeds, sowing the seeds to be detected and the control seeds in a culture pot of a greenhouse, and recording the days for each pea to emerge and the days for the first pair of leaves to be unfolded until all the first pair of leaves of peas are unfolded;
D. using formulas
Respectively calculating the seedling emergence development indexes SEI of the seeds to be detected and the control seeds, wherein D is i The number of days elapsed from sowing to emergence of the ith seed, and N is the number of the sown seeds; using formulas
Respectively calculating first pair leaf expansion indexes (StEI) of the seed to be detected and the control seed, wherein d i The number of days elapsed from sowing of the ith seed to unfolding of a pair of leaves, and N is the number of the sown seeds; the invention selects the seedling emergence development index and the first pair of leaves unfolding index as indexes to construct the measuring method, and the reasons are as follows: the time required by the seedling emergence of the pea seeds and the unfolding of the first pair of the leaves is short, and the linear fitting degree between the two indexes and the neutron radiation dose is optimal.
E. Subtracting 1 from the SEI value of the control seed to obtain a y value, jointly substituting x =0 into formula 1, calculating a b1 value, subtracting 1 from the StEI value of the control seed to obtain a y value, jointly substituting x =0 into formula 2, and calculating a b2 value;
F. substituting the SEI value of the seed to be detected as a y value into a formula 1, calculating the value of x, wherein the value is x1, substituting the STEI value of the seed to be detected as a y value into a formula 2, calculating the value of x, wherein the value is x2, the average value of x1 and x2 is the neutron absorbent dose value of the pea seed in the area to be detected, and the neutron absorbent dose value is the neutron radiation dose value of the area to be detected;
in step E, F, the formula 1 is: y =0.6448x+b1. In the research process of the applicant, the seedling development index SEI of peas and the neutron absorption dose have high linear correlation in the range of 3-10Gy, so that 3 dose points of 3.55Gy, 5.47Gy and 9.27Gy are selected for experimental verification and a linear equation is constructed, and the result shows that the fitting degree of the obtained linear equation is high (R is a linear equation) 2 = 0.9771), in order to expand the applicable range of the linear equation, a control dose of 0Gy is further added for verification, the result is shown in fig. 1, the linear equation is y =0.6448x +12.894 2 =0.8351, the intercept value in the linear equation is replaced by b1, that is, it can be transformed into formula 1, as shown in fig. 1, b1 is the SEI value of the control seed (0 Gy) minus 1, and the applicable dose range of formula 1 is 0-10Gy, which can meet the needs of most detection occasions.
The formula 2 is: y =0.5693x + b2. Same as thatIn the research process, the applicant finds that the first pair of supporting leaf expansion indexes StEI of the peas has higher linear correlation with the neutron absorption dose in the range of 3-10Gy, so that the 3 dose points of 3.55Gy, 5.47Gy and 9.27Gy are selected for experimental verification, and the result shows that the fitting degree of the obtained linear equation is higher (R is R 2 = 0.9739), in order to expand the applicable range of the linear equation, a control dose of 0Gy is further added for verification, and the result is shown in fig. 2, where the linear equation is y =0.5693x +16.47, r 2 =0.8031, the intercept value in the linear equation is replaced by b2, that is, it can be transformed into formula 2, as shown in fig. 2, b2 is the value of the stoei of the control seed (0 Gy) minus 1, and the applicable dose range of formula 2 is 0-10Gy, which can meet the needs of most detection occasions.
Preferably, in the step a, 5 repeated measurement positions are set in the region to be detected, at least 3 seeds are placed in each position, and each seed is kept to be not overlapped with each other, so as to ensure the accuracy of the detection result.
More preferably, in step C, emergence criteria are set as the push of the pea germ out of the soil surface.
Compared with the prior art, the invention has the following beneficial effects:
1. the detection carrier used by the invention is needle pea seeds, and compared with the traditional detection method using a physical detector, the detection carrier has the advantages that the price is only one hundred thousand, even one millionth, and the cost is low. The needle pea seeds belong to green organisms, and are more green and environment-friendly in the subsequent recovery treatment after use compared with physical detectors belonging to electrical devices. In addition, the needle pea seeds have strong resistance, are easy to obtain, do not need special management, and have the effects of reducing management cost and purchasing cost and being convenient to use.
2. Compared with the traditional detection method using nuclear electronics devices, the greenhouse seedling culture experimental method adopted by the invention is easier to learn and master and is convenient to popularize. In the greenhouse experiment process, experimental materials including the needle pea seeds and the like are pollution-free materials, and compared with electronic industrial materials such as nuclear electronics devices and the like, the greenhouse experiment process has the effects of environmental protection and no pollution.
Drawings
FIG. 1 is a linear relationship graph of seedling emergence growth index SEI and neutron absorption dose of peas;
FIG. 2 is a graph of the first versus the linear dependence of the first versus the neutron absorber expansion index STEI for peas;
fig. 3 is a flow chart of neutron radiation dose detection according to the present invention.
Detailed Description
The invention is further illustrated by the following figures and specific examples.
Referring to fig. 3, the invention discloses a neutron radiation dose detection method based on needle pea dry seeds, taking the neutron radiation dose of a californium 252 isotope neutron source as an example, which comprises the following steps: selecting dry needle pea seeds with plump seeds and a germination rate of 97%; uniformly arranging 5 repeated measuring positions in a region to be detected of the neutron radiation dose of the californium 252 isotope neutron source; placing the screened dry needle-leaved pea seeds at 5 repeated measuring positions, placing 15 seeds at each position, and keeping the 15 seeds at each position in single-row arrangement to prevent overlapping; when neutron radiation dose detection is needed, randomly extracting 3 seeds at each repeated measurement position, and then extracting 15 control seeds (seeds free from neutron irradiation); sowing seeds in a region to be detected and control seeds in a culture pot of the same greenhouse, taking the push-out soil surface of pea embryos as a seedling emergence standard, recording the days for seedling emergence of each pea plant and the days for unfolding first pair of leaves until all the first pair of leaves of the peas are unfolded; using formulas
Calculating seedling emergence development index by formula
Calculating a first pair of leaf unfolding indexes, and obtaining results shown in tables 1 and 2; subtracting 1 from the SEI value of the control seed to be used as a y value and x =0 to be jointly substituted into a formula y =0.6448x + b1, calculating the value of b1, subtracting 1 from the StEI value of the control seed to be used as a y value and x =0 to be jointly substituted into a formula y =0.5693x + b2, and calculating the value of b2; substituting the SEI value of the seed to be measured as the y value into a formulaAfter y =0.6448x + b1, the value of x is calculated, which is x1, x1=5.74; substituting the StEI value of the seed to be detected as a y value into a formula y =0.5693x + b2, and calculating a value of x, wherein the value is x2, and x2=5.85; the average value of x1 and x2 is the neutron absorption dose value of the pea seeds in the area to be detected, and the neutron absorption dose value is the neutron radiation dose value of the area to be detected. Therefore, the measured neutron radiation dose value of the californium 252 isotope neutron source is 5.8Gy, the neutron radiation dose value of the same detection area is 6.0Gy detected by the ORTEC neutron detector at the same detection time, and the two detection values are close to each other, so that the detection method has high accuracy and high popularization and application values.
TABLE 1 days for seed emergence and days for first pair of leaves development in the test area
TABLE 2 days for emergence of control seeds and days for first couple of leaves to unfold
Claims (3)
1. A neutron radiation dose detection method based on needle pea dry seeds is characterized by comprising the following steps:
A. selecting needle-leaf pea dry seeds with full seeds and a germination rate of more than 95%, and placing the needle-leaf pea dry seeds in a neutron radiation dose detection area;
B. when neutron radiation dose detection is required, randomly extracting at least 3 seeds subjected to neutron irradiation in the step A;
C. taking the same number of dry seeds which are free from irradiation as the seeds to be detected as control seeds, sowing the seeds to be detected and the control seeds in a culture pot of a greenhouse, and recording the days for each pea to emerge and the days for the first pair of leaves to be unfolded until all the first pair of leaves of peas are unfolded;
D. using formulas
Respectively calculating the seedling emergence and development indexes SEI of the seeds to be detected and the control seeds, wherein D i The number of days elapsed from sowing to emergence of the ith seed, and N is the number of the sown seeds; using formulas
Respectively calculating first pair leaf expansion indexes (StEI) of the seed to be detected and the control seed, wherein d i The number of days elapsed from sowing of the ith seed to unfolding of a pair of leaves, and N is the number of the sown seeds;
E. subtracting 1 from the SEI value of the control seed to be used as a y value and x =0 to be jointly substituted into a formula 1, calculating a value of b1, subtracting 1 from the StEI value of the control seed to be used as a y value and x =0 to be jointly substituted into a formula 2, and calculating a value of b2;
F. substituting the SEI value of the seed to be detected as a y value into a formula 1, calculating the value of x, wherein the value is x1, substituting the STEI value of the seed to be detected as a y value into a formula 2, calculating the value of x, wherein the value is x2, taking the average value of x1 and x2 as the neutron absorption dose value of the pea seed in the area to be detected, and the neutron absorption dose value is the neutron radiation dose value of the area to be detected;
in step E, F, the formula 1 is: y =0.6448x+b1, formula 2 is: y =0.5693x + b2; the value range of the x is 3-10Gy.
2. The method of claim 1, wherein in step a, 5 repeated measuring positions are provided in the area to be detected, each position containing at least 3 seeds, and each seed is not overlapped with each other.
3. The neutron radiation dose detection method based on the dry needle pea seeds as claimed in claim 1 or 2, wherein in the step C, the soil pushing-out of the pea embryo is taken as a standard for emergence.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| JPS63154968A (en) * | 1986-12-19 | 1988-06-28 | Riken Kagaku Kogyo Kk | Detection for dry onion irradiated with radiation |
| WO2003030638A2 (en) * | 2001-10-05 | 2003-04-17 | Millennial Technology, Inc. | A method of inhibiting sprouting in plant products |
| CN107422363A (en) * | 2017-08-25 | 2017-12-01 | 兰州大学 | It is a kind of for vegetable seeds neutron irradiation252Cf sources dosage distribution irradiation devices |
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- 2021-02-07 CN CN202110179745.9A patent/CN112946724B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63154968A (en) * | 1986-12-19 | 1988-06-28 | Riken Kagaku Kogyo Kk | Detection for dry onion irradiated with radiation |
| WO2003030638A2 (en) * | 2001-10-05 | 2003-04-17 | Millennial Technology, Inc. | A method of inhibiting sprouting in plant products |
| CN107422363A (en) * | 2017-08-25 | 2017-12-01 | 兰州大学 | It is a kind of for vegetable seeds neutron irradiation252Cf sources dosage distribution irradiation devices |
Non-Patent Citations (5)
| Title |
|---|
| ~(252)Cf裂变中子辐照玉米种子生物学效应初步研究;刘忠祥等;《华北农学报》;20161028(第05期);020401-1-7 * |
| ~(252)Cf裂变中子辐照豌豆种子生物学效应的初步研究;徐大鹏等;《核技术》;20131110(第11期);110207-1-5 * |
| 不同剂量中子辐射胡麻种子的M_1和M_2代生物学效应研究;徐大鹏等;《核技术》;20170210(第02期);020203-1-7 * |
| 不同剂量中子辐射针叶豌豆的M1代效应研究;徐大鹏等;《中国农学通报》;20150425(第12期);200-204 * |
| 快中子辐照豌豆种子的生物学效应研究;徐大鹏;《中国优秀硕士学位论文全文数据库 (农业科技辑)》;20120715;13-36 * |
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