CN112946281A - Test strip for rapidly detecting African swine fever virus and detection method thereof - Google Patents
Test strip for rapidly detecting African swine fever virus and detection method thereof Download PDFInfo
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
- G01N33/587—Nanoparticles
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
- G01N2333/01—DNA viruses
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2469/00—Immunoassays for the detection of microorganisms
- G01N2469/10—Detection of antigens from microorganism in sample from host
Abstract
The invention discloses a test strip for rapidly detecting African swine fever virus, which comprises a support plate, wherein a sample pad, a reagent pad, a detection area and a water absorption pad are sequentially adhered to the surface of the support plate from a test end to a water absorption end. The use method of the test strip comprises the following steps: collecting a sample to be detected, adding phosphate buffer solution or water, and mixing uniformly; dripping a sample pad of the test strip; horizontally placing for 5-10 minutes, dropwise adding a copper chloride ethanol solution at the detection line and the control line, and observing the result. The test strip can directly utilize whole pig blood and body fluid for detection in the whole process of detecting the African swine fever, has the advantages of convenience, rapidness, economy, sensitivity, specificity and the like, is particularly suitable for on-site African swine fever virus detection, epidemiological investigation and live pig international trade quarantine, and has practical application significance.
Description
Technical Field
The invention belongs to the technical field of immunoassay and animal infection diagnosis, and particularly relates to a test strip for rapidly detecting African swine fever virus and a detection method thereof.
Background
African swine fever is an acute, hemorrhagic and virulent infectious disease caused by African Swine Fever Virus (ASFV) infecting domestic pigs and various wild pigs (such as African wild pigs, European wild pigs, etc.). The world animal health organization classifies the animal epidemic disease as a legal report animal epidemic disease, and the disease is also a kind of animal epidemic situation which is mainly prevented in China. The clinical symptoms of African swine fever are similar to those of swine fever, and diagnosis can be confirmed only by means of laboratory monitoring.
The symptoms and lesions of African swine fever are similar to those of other hemorrhagic diseases of swine fever, and their subacute and chronic forms are practically indistinguishable at the production site and must be identified by laboratory methods. Currently, there are several categories of laboratory testing methods: red blood cell adsorption test; direct immunofluorescence assay; animal inoculation test; an indirect immunofluorescence assay; enzyme-linked immunosorbent assay; performing an immunoelectrophoresis test; indirect enzyme linked immunosorbent plaque assay. However, the detection method has the defects of long detection period, high requirement on detection instruments and the like, and is particularly not suitable for on-site detection of ASFV.
Disclosure of Invention
In order to solve the defect that most African swine fever virus detection methods in the prior art can only rely on laboratory detection, the invention provides the test strip for rapidly detecting African swine fever virus and the detection method thereof, and the test strip has the advantages of safer, more reliable and quicker use, and solves the problems that special reagent equipment is needed when ASFV is detected by other methods in the prior art, and laboratory detection is needed.
In order to achieve the purpose, the invention provides the following technical scheme: the invention provides a test strip for rapidly detecting African swine fever virus, which comprises a support plate, wherein a sample pad, a reagent pad, a detection area and a water absorption pad are sequentially adhered to the surface of the support plate from a test end to a water absorption end; the reagent pad is adsorbed with a protein monoclonal antibody marked by 3,3',5,5' -tetramethylbenzidine nano particles; the detection area is provided with a test line and a control line, the test line is adsorbed with a protein monoclonal antibody, and the control line is adsorbed with immunoglobulin; the test strip also comprises a color developing agent which is used for dripping on the detection area before detection. According to the test strip prepared by the invention, the TMB nano-particles are prepared, the loading capacity of TMB can be greatly improved, and the amino group of the TMB molecule can be coordinated with copper ions in an ethanol solution to form a yellow copper ion complex. TMB molecules and copper ions have extremely high coordination speed, and obvious color change can be observed within half a minute, so that rapid and accurate field detection is realized.
Further, the preparation method of the reagent pad comprises the following steps:
(1) adding 25mg of PEG, 1mg of BSA and 0.1mg of protein monoclonal antibody into 10mL of 0.01mol/L phosphorus buffer solution with the pH of 7.4 at the temperature of 4 ℃, slowly dropwise adding 2mL of DMSO solution containing 5mg/mL of TMB under magnetic stirring, and continuously stirring for 6 hours after the addition of the TMB solution is finished;
(2) centrifuging at 4 deg.C and 5000rpm for three times, lyophilizing, and processing at 2 μ L/cm2The spraying amount of the 3,3',5,5' -tetramethyl bi-compound prepared in the step (1) is 2mg/mLAnd spraying the protein monoclonal antibody marked by the aniline nanoparticles on a glass fiber membrane, and drying in an oven at 37 ℃ for 1h to obtain the reagent pad.
Further, the protein monoclonal antibody is any one of capsid protein P72 protein monoclonal antibody, P54 protein monoclonal antibody, P32 protein monoclonal antibody and penton protein monoclonal antibody.
Further, the particle size range of the 3,3',5,5' -tetramethyl benzidine nano particles adsorbed by the reagent pad is 50nm-150 nm.
Furthermore, the concentration of the protein monoclonal antibody on the test line is 1.0mg/mL, and the concentration of the immunoglobulin on the control line is 1.0 mg/mL.
Further, the color developing agent is any one of a copper chloride ethanol solution, a copper bromide ethanol solution, a copper chloride acetonitrile solution, a copper bromide acetonitrile solution and a methyl sulfoxide solution, and the concentration of the color developing agent is 1-10 mM.
The invention also provides a detection method, which comprises the following steps:
(1) collecting a sample to be detected, adding 100 mu L of pig whole blood or pig body fluid into 400 mu L of sample diluent, and fully and uniformly mixing for 1 min; the sample diluent is PBS or water;
(2) dripping 200 mu L of the diluted sample to be detected into a sample pad, and horizontally placing for 5 min;
(3) dripping 1 mu L of color developing agent in the test area of the test strip, and if two yellow strips appear simultaneously on the test line T and the control line C of the test strip, indicating that the sample to be tested contains African swine fever virus; if the test strip detection line T and the control line C only show a yellow strip, the test strip detection line T and the control line C indicate that the sample to be detected does not contain African swine fever virus; if the test strip does not have any strip, the test operation is improper or the test card fails.
In conclusion, the test strip provided by the invention has the beneficial effects that the test strip can directly utilize whole pig blood and body fluid for detection in the whole process of detecting African swine fever, has the advantages of convenience, rapidness, economy, sensitivity, specificity and the like, is particularly suitable for on-site African swine fever virus detection, epidemiological investigation and live pig international trade quarantine, and has practical application significance.
Drawings
Fig. 1 is a transmission electron microscope image of the reagent pad adsorbed with TMB nanoparticle labeled P72 protein monoclonal antibody in example 1 of the present invention.
FIG. 2 is a schematic structural diagram of a reagent strip in embodiment 1 of the present invention.
Detailed Description
The present invention is described in further detail below with reference to specific examples. The starting materials referred to in the examples are all commercially available.
The first embodiment is as follows: the invention provides a test strip for rapidly detecting African swine fever virus, which comprises a PVC (polyvinyl chloride) support plate, wherein a sample pad, a reagent pad, a detection area and a water absorption pad are adhered to the surface of the support plate in sequence from a test end to a water absorption end; the reagent pad is adsorbed with a P72 protein monoclonal antibody labeled by 3,3',5,5' -tetramethylbenzidine nano particles; the detection area is provided with a test line and a control line, the test line is adsorbed with a P72 protein monoclonal antibody, and the control line is adsorbed with immunoglobulin; the test paper strip also comprises a color developing agent, the color developing agent is used for being dripped on a detection area before detection, and the reagent pad adsorbs the P72 protein monoclonal antibody marked by TMB nano particles as shown in figure 1.
Further, the preparation method of the reagent pad comprises the following steps:
(1) adding 25mg of PEG, 1mg of BSA and 0.1mg of P72 protein monoclonal antibody into 10mL of 0.01mol/L phosphorus buffer solution with the pH of 7.4 at the temperature of 4 ℃, slowly dropwise adding 2mL of DMSO solution containing 5mg/mL of TMB under magnetic stirring, and continuously stirring for 6 hours after the addition of the TMB solution is finished;
(2) washing three times at 4 ℃ by centrifugation at 5000rpm, and freeze-drying for later use. At 2. mu.L/cm2The spraying amount of the reagent is that 2mg/mL TMB nano-particle redissolution is sprayed on a glass fiber membrane and dried in an oven at 37 ℃ for 1h to obtain the reagent pad.
Further, the particle size range of the TMB nanoparticle-labeled P72 protein monoclonal antibody adsorbed by the reagent pad is 50nm-150 nm.
Furthermore, the concentration of the P72 protein monoclonal antibody on the test line is 1.0mg/mL, the concentration of the immunoglobulin on the control line is 1.0mg/mL, and the distance between the test line and the control line is 5 mm.
Glass fiber cotton is used as a sample pad, and is soaked in 1xPBS solution of 0.2 percent Tween 20(v/v), dried at room temperature after being dried in vacuum at 40 ℃ and stored.
Further, the color developing agent is any one of a copper chloride ethanol solution, a copper bromide ethanol solution, a copper chloride acetonitrile solution, a copper bromide acetonitrile solution and a dimethyl sulfoxide solution, and the concentration of the color developing agent is 1-10 mM. In this embodiment, the developer is an ethanol solution of cupric chloride, and the concentration is 10 mM.
The structure and appearance of the test strip are shown in fig. 2, and the test strip is assembled as follows:
(1) adhering a 25mm detection film to a 66mm PVC plastic bottom plate;
(2) adsorbing 18mm long water absorption filter paper, and overlapping the water absorption filter paper and the detection membrane for 2 mm;
(3) adsorbing a reagent pad with the length of 10mm, and overlapping the reagent pad and the detection membrane for 2 mm;
(4) the sample pad is 15mm long and overlaps 2mm with the reagent pad;
(5) the assembled reagent strip was cut into 4mm wide strips, which could be sheathed with a plastic protective shell.
The test strip is used for detecting the African swine fever virus, and the detection comprises the following steps:
(1) collecting a sample to be detected, adding 100 mu L of pig whole blood or pig body fluid into 400 mu L of sample diluent, and fully and uniformly mixing for 1 min;
(2) dripping 200 mu L of the diluted sample to be detected into a sample pad, and horizontally placing for 5 min;
(3) drop-coating a color developing agent in the test area of the test strip, and if two yellow strips appear simultaneously on the test line T and the control line C of the test strip, indicating that the sample to be tested contains African swine fever virus; if the test strip detection line T and the control line C only show a yellow strip, the test strip detection line T and the control line C indicate that the sample to be detected does not contain African swine fever virus; if the test strip does not have any strip, the test operation is improper or the test card fails. The detection principle of the invention is TMB and Cu2+Coordination color development:
specificity and sensitivity test:
the PCR detection result is used as a standard. 10 parts of ASFV P72 protein negative pig whole blood, 20 parts of ASFV P72 protein weak positive whole blood and 20 parts of ASFV P72 protein strong positive whole blood after ASFV artificial infection are selected, and the test strip is adopted to carry out detection according to the procedures. The results show that: the negative coincidence rate of the ASFVP72 protein detected by the reagent strip is 90% (9/10), and the coincidence rate of weak positive and strong positive is 100%.
Reproducibility test whole blood:
the 10 parts of ASFV P72 protein whole blood, 20 parts of ASFV P72 protein weak positive and 20 parts of ASFV P72 protein strong positive whole blood are subjected to 3 times of repeated detection, and the result shows that the detection results of each part of whole blood are consistent, which indicates that the test strip has good repeatability.
It should be noted that the protein monoclonal antibody of the present application can be replaced by the P54 protein monoclonal antibody or the P32 protein monoclonal antibody or the penton protein monoclonal antibody, and the color developing agent can be replaced by the copper bromide ethanol solution or the copper chloride acetonitrile solution or the copper bromide acetonitrile solution or the dimethylsulfoxide solution with the concentration of 1-10mM, for example, with the concentration of 1mM, 5mM, 2mM, 8mM, in the actual test, the effect is consistent with that of the first embodiment, the accuracy of the test result is greater than 90%, and the test results are consistent and have good repeatability in 3 times of repeated parallel tests, so as to reduce unnecessary repetition and save space, the embodiments are not listed.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (7)
1. A test strip for rapidly detecting African swine fever virus is characterized by comprising a support plate, wherein a sample pad, a reagent pad, a detection area and a water absorption pad are sequentially adhered to the surface of the support plate from a test end to a water absorption end; the reagent pad is adsorbed with a protein monoclonal antibody marked by 3,3',5,5' -tetramethylbenzidine nano particles; the detection area is provided with a test line and a control line, the test line is adsorbed with a protein monoclonal antibody, and the control line is adsorbed with immunoglobulin; the test strip also comprises a color developing agent which is used for dripping on the detection area before detection.
2. The test strip of claim 1, wherein the reagent pad is prepared by the steps of:
(1) adding 25mg of PEG, 1mg of BSA and 0.1mg of protein monoclonal antibody into 10mL of 0.01mol/L phosphorus buffer solution with the pH of 7.4 at the temperature of 4 ℃, slowly dropwise adding 2mL of DMSO solution containing 5mg/mL of TMB under magnetic stirring, and continuously stirring for 6 hours after the addition of the TMB solution is finished;
(2) centrifuging at 4 deg.C and 5000rpm for three times, lyophilizing, and processing at 2 μ L/cm2The spraying amount of the reagent pad is obtained by spraying 2mg/mL of the 3,3',5,5' -tetramethylbenzidine nanoparticle-labeled protein monoclonal antibody prepared in the step (1) on a glass fiber membrane and drying the monoclonal antibody in an oven at 37 ℃ for 1 h.
3. The test strip of claim 1, wherein the protein monoclonal antibody is any one of capsid protein P72 protein monoclonal antibody, P54 protein monoclonal antibody, P32 protein monoclonal antibody and penton protein monoclonal antibody.
4. The test strip of claim 1, wherein the 3,3',5,5' -tetramethylbenzidine nanoparticles adsorbed by the reagent pad have a particle size ranging from 50nm to 150 nm.
5. The test strip of claim 1, wherein the concentration of the protein monoclonal antibody on the test line is 1.0mg/mL, and the concentration of the immunoglobulin on the control line is 1.0 mg/mL.
6. The test strip of claim 1, wherein the color-developing agent is any one of a cupric chloride ethanol solution, a cupric bromide ethanol solution, a cupric chloride acetonitrile solution, a cupric bromide acetonitrile solution, and a dimethyl sulfoxide solution, and the concentration of the color-developing agent is 1-10 mM.
7. A detection method using the test strip of any one of claims 1 to 6, wherein the detection comprises the following steps:
(1) collecting a sample to be detected, adding 100 mu L of pig whole blood or pig body fluid into 400 mu L of sample diluent, and fully and uniformly mixing for 1 min;
(2) dripping 200 mu L of the diluted sample to be detected into a sample pad, and horizontally placing for 5 min;
(3) dripping 1 mu L of color developing agent in the test area of the test strip, and if two yellow strips appear simultaneously on the test line T and the control line C of the test strip, indicating that the sample to be tested contains African swine fever virus; if the test strip detection line T and the control line C only show a yellow strip, the test strip detection line T and the control line C indicate that the sample to be detected does not contain African swine fever virus; if the test strip does not have any strip, the test operation is improper or the test card fails.
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104198470A (en) * | 2014-08-04 | 2014-12-10 | 中国科学院宁波材料技术与工程研究所 | Array sensor used for detecting heavy metal ions as well as preparation method and application thereof |
| CN104407131A (en) * | 2014-10-22 | 2015-03-11 | 中国检验检疫科学研究院 | Immunomagnetic bead substrate color development test paper and preparation method thereof |
| US20160299136A1 (en) * | 2013-12-13 | 2016-10-13 | Nanobiz Nanobiyoteknolojik Sistemler Egitim Bilisim Danismanlik Ar-Ge San.Tic. Ltd. Sti. | Aptamer-gated nanoparticles for lateral flow assays |
| CN107290540A (en) * | 2017-06-22 | 2017-10-24 | 中国农业大学 | Escherichia coli O 157:H7 and the common Test paper of salmonella typhimurium and preparation method and application |
| CN109254155A (en) * | 2018-09-25 | 2019-01-22 | 扬州大学 | A kind of detection African swine fever virus antigen colloidal gold immune chromatography test paper and preparation method and application |
| CN112098656A (en) * | 2020-08-25 | 2020-12-18 | 广州优迪生物科技股份有限公司 | HRP immunochromatographic reagent strip for detecting human SARS-CoV-2 and preparation method thereof |
-
2021
- 2021-01-25 CN CN202110095511.6A patent/CN112946281B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20160299136A1 (en) * | 2013-12-13 | 2016-10-13 | Nanobiz Nanobiyoteknolojik Sistemler Egitim Bilisim Danismanlik Ar-Ge San.Tic. Ltd. Sti. | Aptamer-gated nanoparticles for lateral flow assays |
| CN104198470A (en) * | 2014-08-04 | 2014-12-10 | 中国科学院宁波材料技术与工程研究所 | Array sensor used for detecting heavy metal ions as well as preparation method and application thereof |
| CN104407131A (en) * | 2014-10-22 | 2015-03-11 | 中国检验检疫科学研究院 | Immunomagnetic bead substrate color development test paper and preparation method thereof |
| CN107290540A (en) * | 2017-06-22 | 2017-10-24 | 中国农业大学 | Escherichia coli O 157:H7 and the common Test paper of salmonella typhimurium and preparation method and application |
| CN109254155A (en) * | 2018-09-25 | 2019-01-22 | 扬州大学 | A kind of detection African swine fever virus antigen colloidal gold immune chromatography test paper and preparation method and application |
| CN112098656A (en) * | 2020-08-25 | 2020-12-18 | 广州优迪生物科技股份有限公司 | HRP immunochromatographic reagent strip for detecting human SARS-CoV-2 and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 惠生武 主编: "《公安学科实验教学指导》", 31 July 2012, 中国政法大学出版社 * |
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