CN112934486A

CN112934486A - Biological cell separation is with from fastening auxiliary assembly who holds automatic deceleration of stall

Info

Publication number: CN112934486A
Application number: CN202110125185.9A
Authority: CN
Inventors: 刘冀中
Original assignee: Liuzhou Renjia Biotechnology Co ltd
Current assignee: Liuzhou Renjia Biotechnology Co ltd
Priority date: 2021-01-29
Filing date: 2021-01-29
Publication date: 2021-06-11

Abstract

The invention relates to the technical field of biological cell separation and discloses self-fastening clamping stall automatic deceleration auxiliary equipment for biological cell separation, which comprises a centrifugal shaft, wherein a deceleration block is movably connected to the outer part of the centrifugal shaft, a deceleration plate is movably connected to the outer part of the deceleration block, a deceleration magnet is movably connected to the inner part of the deceleration plate, an adjusting spring is movably connected to the top of the deceleration plate, an electrified coil is movably connected to the top of the adjusting spring, a piezoresistor is movably connected to the left side of the electrified coil, when the centrifugal shaft stalls excessively fast, the rotating speed of a cutting coil is increased, so that the intensity of induced current is increased, electricity flows into the electrified coil through the piezoresistor to generate magnetism, and the electrified coil can generate repulsion force on the deceleration magnet so as to stretch the adjusting spring to drive the deceleration plate to move until the deceleration plate is contacted with the deceleration block, thereby achieving the effect of automatic speed reduction if the stalling phenomenon occurs in the process of cell centrifugation.

Description

Biological cell separation is with from fastening auxiliary assembly who holds automatic deceleration of stall

Technical Field

The invention relates to the technical field of biological cell separation, in particular to an auxiliary device for self-fastening clamping stall automatic speed reduction for biological cell separation.

Background

With the rapid development of science and technology, people are helped to research various fields, the biological field is one of the research fields which people pay special attention to, people can develop medicaments for resisting various diseases through the research on the biological field, people now develop various vaccines, and the average life level of people is improved.

In the process of carrying out biological research, often can use centrifuge to carry out differential separation to biological cell to obtain different cell material, traditional centrifugal equipment is though there is the stall protection device, but its protection zone is great, and the cell in the test tube also can be destroyed when triggering protection device this moment, thereby causes the loss of material, and traditional equipment passes through the mode of screw thread to the test tube simultaneously and fixes, and this kind of fixed mode very easily takes place to drop when high-speed centrifugation, causes the sample to destroy.

In view of the above, the present invention provides an auxiliary device for self-fastening clamping stall automatic deceleration for biological cell separation, which is developed and improved in view of the existing structure and defects, so as to achieve the purpose of higher practical value.

Disclosure of Invention

Technical problem to be solved

Aiming at the defects of the prior art, the invention provides self-fastening clamping stall automatic deceleration auxiliary equipment for separating biological cells, which has the advantages of automatically decelerating if a stall phenomenon occurs in the cell centrifugation process and automatically fastening a test tube in the cell centrifugation process, and solves the problems that the equipment is damaged and cannot be accurately protected when the traditional centrifugal equipment triggers a stall protection mechanism, and the test tube is easy to fall off in the centrifugation process.

(II) technical scheme

In order to realize the purposes of automatically reducing the speed and automatically fastening the test tube in the process of centrifuging the cells if the stalling phenomenon occurs in the process of centrifuging the cells, the invention provides the following technical scheme: the utility model provides a biological cell separation is with from automatic auxiliary assembly that slows down of tight clamp-holding stall, includes the centrifugal axle, the outside swing joint of centrifugal axle has the piece of slowing down, the outside swing joint of piece of slowing down has the speed reduction board, the inside swing joint of speed reduction board has speed reduction magnet, speed reduction board top swing joint has adjusting spring, adjusting spring top swing joint has electrical coil, electrical coil left side swing joint has piezo-resistor, the outside swing joint of electrical coil has cutting coil. The utility model discloses a cutting coil, including cutting coil, power-on circuit, clamping spring top swing joint have the transfer line, the outside swing joint of cutting coil has the power-on circuit, the outside swing joint of power-on circuit has the magnetic sheet, the outside swing joint of magnetic sheet has the connecting seat, the outside fixedly connected with centre gripping wheel of connecting seat, the inside swing joint of centre gripping wheel has the fastening axle, the outside swing joint of fastening axle has centre gripping magnet, centre gripping magnet top swing joint has the centre gripping spring, centre gripping spring top swing joint has the transfer line, transfer line top swing joint has adjusting magnet, adjusting magnet top swing joint has the extrusion piston, extrusion piston top swing joint has the gas storage chamber, the outside swing joint in gas storage chamber has.

Preferably, the magnetism generated when the clamping magnet is electrified is the same as the magnetism of one end of the adjusting magnet close to the clamping magnet.

Preferably, the cutting coil forms a closed loop with the energizing coil, the piezoresistor and the clamping magnet through an energizing circuit, and the energizing coil is connected with the piezoresistor in series and connected with the clamping magnet in parallel.

Preferably, the diameter of the extrusion piston is consistent with that of the inner wall of the air storage cavity.

Preferably, the width of the mounting seat is consistent with the thickness of the outer wall of the test tube.

Preferably, the magnetism generated when the energizing coil is energized is the same as the magnetism of one end of the decelerating magnet close to the energizing coil.

(III) advantageous effects

Compared with the prior art, the invention provides an auxiliary device for automatically reducing the speed of self-fastening clamping stall for biological cell separation, which has the following beneficial effects:

1. the self-fastening clamping stall automatic speed reduction auxiliary equipment for separating the biological cells drives the centrifugal shaft to rotate by an external driving source electricity during normal use so as to carry out differential centrifugation on the cells, at the moment, the centrifugal shaft rotates to drive the cutting coil to rotate simultaneously, the cutting coil rotates to cut the magnetic induction line between the two magnetic plates to generate induction current, the current flows into the piezoresistor through the electric circuit, the current generated by the cutting coil for cutting the magnetic induction line during normal rotation is smaller, so that the voltage applied to the piezoresistor is smaller than the threshold value of the piezoresistor, the piezoresistor is equivalent to a disconnected switch, when the stall of the centrifugal shaft occurs too fast, the rotating speed of the cutting coil is increased at the moment so that the intensity of the induction current is increased, at the moment, the voltage applied to the piezoresistor is larger than the threshold value of the piezoresistor, so that the piezoresistor is equivalent to a closed switch, current flows into the electrified coil through the piezoresistor, make the electrified coil circular telegram produce magnetism, the electrified coil can produce a repulsion to the deceleration magnet this moment, this repulsion is used in on adjusting spring through the deceleration board, thereby tensile adjusting spring makes it take place to drive the deceleration board after deformation and move until deceleration board and the contact of retardation piece, the deceleration board can apply a resistance to the retardation piece after the contact of retardation piece and prevent it from continuing to rotate until it stops, and then prevent the rotation of centrifugal shaft, thereby reached if the automatic effect of slowing down of phenomenon takes place for stall at the in-process of cell centrifugation.

2. The self-fastening clamping stall automatic speed reduction auxiliary equipment for separating the biological cells is characterized in that the top of an artificial test tube is required to be installed in a mounting seat before cell centrifugation is carried out, the artificial test tube is preliminarily fixed through an extrusion air bag, a centrifugal shaft rotates to drive a cutting coil to rotate to cut a magnetic induction line to generate induction current along with the start of centrifugation work, the current flows into a clamping magnet through a power line, so that the clamping magnet is electrified to generate magnetism, at the moment, an adjusting magnet can receive repulsive force from the clamping magnet, the repulsive force acts on a clamping spring through a transmission rod, and finally, the clamping spring is stretched to deform and then drive an extrusion piston to move, the extrusion piston can extrude the inner space of an air storage cavity, so that the inner space of the air storage cavity is reduced, at the moment, gas in the air storage cavity can be injected into an expansion air cavity under the action of the extrusion piston, so that the inner pressure of the expansion, the pressure intensity of increase can be applyed an effort for extrusion gasbag, and the extrusion gasbag can take place the inflation after the atress, applys bigger clamping-force to the test tube to reached and carried out the automatic effect to the test tube fastening of centrifugal in-process to the cell.

Drawings

FIG. 1 is a schematic view of a connecting base according to the present invention;

FIG. 2 is a schematic view of a magnetic plate structure according to the present invention;

FIG. 3 is a schematic view of a clamping wheel according to the present invention;

FIG. 4 is a schematic view of a cutting coil according to the present invention;

fig. 5 is a schematic view of the structure of the expansion air cavity of the present invention.

In the figure: 1. a centrifugal shaft; 2. a retarding block; 3. a speed reduction plate; 4. a deceleration magnet; 5. adjusting the spring; 6. an electrified coil; 7. a voltage dependent resistor; 8. cutting the coil; 9. a power-on circuit; 10. a magnetic plate; 11. a connecting seat; 12. a clamping wheel; 13. fastening the shaft; 14. a clamping magnet; 15. a clamping spring; 16. a transmission rod; 17. adjusting the magnet; 18. a squeeze piston; 19. a gas storage cavity; 20. an expansion air cavity; 21. extruding the air bag; 22. and (7) mounting a seat.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-5, an auxiliary device for self-fastening clamping stall automatic deceleration for biological cell separation comprises a centrifugal shaft 1, a deceleration block 2 is movably connected to the outside of the centrifugal shaft 1, a deceleration plate 3 is movably connected to the outside of the deceleration block 2, a deceleration magnet 4 is movably connected to the inside of the deceleration plate 3, an adjusting spring 5 is movably connected to the top of the deceleration plate 3, an energizing coil 6 is movably connected to the top of the adjusting spring 5, the magnetism generated when the energizing coil 6 is energized is the same as the magnetism of one end of the deceleration magnet 4 close to the energizing coil 6, a piezoresistor 7 is movably connected to the left side of the energizing coil 6, and a cutting coil 8 is movably connected to the outside of the energizing coil 6. The external part of the cutting coil 8 is movably connected with a power-on circuit 9, the external part of the power-on circuit 9 is movably connected with a magnetic plate 10, the external part of the magnetic plate 10 is movably connected with a connecting seat 11, the external part of the connecting seat 11 is fixedly connected with a clamping wheel 12, the internal part of the clamping wheel 12 is movably connected with a fastening shaft 13, the external part of the fastening shaft 13 is movably connected with a clamping magnet 14, the cutting coil 8 forms a closed loop with the power-on coil 6, a piezoresistor 7 and the clamping magnet 14 through the power-on circuit 9, the power-on coil 6 is connected with the piezoresistor 7 in series and is connected with the clamping magnet 14 in parallel, the magnetism generated when the clamping magnet 14 is powered on is the same as the magnetism of one end of the adjusting magnet 17 close to the clamping magnet 14, the top of the clamping magnet 14 is movably connected with a clamping spring 15, the diameter size of extrusion piston 18 is unanimous with the diameter size of 19 inner walls of gas storage chamber, and extrusion piston 18 top swing joint has gas storage chamber 19, and the outside swing joint of gas storage chamber 19 has inflation air cavity 20, and the outside swing joint of inflation air cavity 20 has extrusion gasbag 21, and the outside swing joint of extrusion gasbag 21 has mount pad 22, and the width of mount pad 22 is unanimous with the thickness size of test tube outer wall.

The varistor characteristic is that when the voltage applied to the varistor is lower than its threshold value, the current flowing through it is extremely small, it is equivalent to a resistance of infinite resistance, when the voltage applied to it is lower than its threshold value, it is equivalent to a switch in the off state; when the voltage applied to the varistor exceeds its threshold value, the current flowing through it increases sharply, which corresponds to a resistor of infinite resistance, i.e. when the voltage applied to it is above its threshold value, it corresponds to a switch in the closed state.

The working principle is as follows: the self-fastening clamping stall automatic speed reduction auxiliary equipment for separating the biological cells is characterized in that an external driving source electrically drives a centrifugal shaft 1 to rotate so as to carry out differential centrifugation on the cells in normal use, at the moment, the centrifugal shaft 1 rotates and drives a cutting coil 8 to rotate simultaneously, the cutting coil 8 rotates and cuts a magnetic induction line between two magnetic plates 10 to generate induction current, the current flows into a piezoresistor 7 through a power transmission line 9, the current generated by cutting the magnetic induction line by the cutting coil 8 in normal rotation is smaller, so that the voltage applied to the piezoresistor 7 is smaller than the threshold value thereof, the piezoresistor 7 is equivalent to a disconnected switch at the moment, when the centrifugal shaft 1 stalls too fast, the rotation speed of the cutting coil 8 is increased so that the intensity of the induction current is increased, at the moment, the voltage applied to the piezoresistor 7 is larger than the threshold value thereof, so that the piezoresistor 7 is equivalent to a closed switch at the moment, current flows into the electrified coil 6 through the piezoresistor 7, so that the electrified coil 6 is electrified to generate magnetism, the electrified coil 6 can generate a repulsive force on the decelerating magnet 4 at the moment, the repulsive force acts on the adjusting spring 5 through the decelerating plate 3, the adjusting spring 5 is stretched to enable the decelerating plate 3 to move until the decelerating plate 3 is contacted with the decelerating block 2 after being deformed, and the decelerating plate 3 can apply a resistance to the decelerating block 2 to prevent the decelerating block 2 from continuing to rotate until the decelerating block stops after the decelerating plate 3 is contacted with the decelerating block 2, so that the rotation of the centrifugal shaft 1 is prevented, and the effect of automatic deceleration if a stall phenomenon occurs in the cell centrifugation process is achieved.

The self-fastening clamping stall automatic speed reduction auxiliary equipment for separating the biological cells is characterized in that the top of an artificial test tube is required to be installed in an installation seat 22 before cell centrifugation is carried out, the artificial test tube is preliminarily fixed through an extrusion air bag 21, a centrifugal shaft 1 rotates to drive a cutting coil 8 to rotate and cut a magnetic induction line to generate induction current along with the start of centrifugation work, the current flows into a clamping magnet 14 through a power line 9, the clamping magnet 14 is electrified to generate magnetism, at the moment, an adjusting magnet 17 receives repulsive force from the clamping magnet 14, the repulsive force acts on a clamping spring 15 through a transmission rod 16, finally, the clamping spring 15 is stretched to deform and then drives an extrusion piston 18 to move, the extrusion piston 18 moves to extrude the inner space of an air storage cavity 19, so that the inner space of the air storage cavity 19 is reduced, at the moment, air in the air storage cavity 19 is injected into an expansion air cavity 20 under the action of the extrusion piston 18, thereby make the inside pressure increase of inflation air cavity 20, the effort of extrusion gasbag 21 can be applyed to the pressure of increase, and extrusion gasbag 21 can take place the inflation after the atress, exerts bigger clamping-force to the test tube to the effect of carrying out the automatic test tube fastening to the in-process of centrifugation to the cell has been reached.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. An auxiliary device for self-fastening clamping stall automatic deceleration for biological cell separation, which comprises a centrifugal shaft (1), and is characterized in that: centrifugal shaft (1) outside swing joint has retarber (2), outside swing joint of retarber (2) has slowdown board (3), inside swing joint of slowdown board (3) has slowdown magnet (4), slowdown board (3) top swing joint has adjusting spring (5), adjusting spring (5) top swing joint has electrical coil (6), electrical coil (6) left side swing joint has piezo-resistor (7), electrical coil (6) outside swing joint has cutting coil (8). The cutting coil (8) is externally and movably connected with a power-on circuit (9), the power-on circuit (9) is externally and movably connected with a magnetic plate (10), the magnetic plate (10) is externally and movably connected with a connecting seat (11), the connecting seat (11) is externally and fixedly connected with a clamping wheel (12), the clamping wheel (12) is internally and movably connected with a fastening shaft (13), the fastening shaft (13) is externally and movably connected with a clamping magnet (14), the top of the clamping magnet (14) is movably connected with a clamping spring (15), the top of the clamping spring (15) is movably connected with a transmission rod (16), the top of the transmission rod (16) is movably connected with an adjusting magnet (17), the top of the adjusting magnet (17) is movably connected with an extruding piston (18), the top of the extruding piston (18) is movably connected with a gas storage cavity (19), and the outside of the gas storage cavity (, the outside of the expansion air cavity (20) is movably connected with an extrusion air bag (21), and the outside of the extrusion air bag (21) is movably connected with a mounting seat (22).

2. The self-fastening stall-auto-deceleration auxiliary device for biological cell separation according to claim 1, wherein: the magnetism generated when the clamping magnet (14) is electrified is the same as the magnetism of one end of the adjusting magnet (17) close to the clamping magnet (14).

3. The self-fastening stall-auto-deceleration auxiliary device for biological cell separation according to claim 1, wherein: the cutting coil (8) forms a closed loop with the electrified coil (6), the piezoresistor (7) and the clamping magnet (14) through the electrified circuit (9), and the electrified coil (6) is connected with the piezoresistor (7) in series and connected with the clamping magnet (14) in parallel.

4. The self-fastening stall-auto-deceleration auxiliary device for biological cell separation according to claim 1, wherein: the diameter of the extrusion piston (18) is consistent with that of the inner wall of the air storage cavity (19).

5. The self-fastening stall-auto-deceleration auxiliary device for biological cell separation according to claim 1, wherein: the width of the mounting seat (22) is consistent with the thickness of the outer wall of the test tube.

6. The self-fastening stall-auto-deceleration auxiliary device for biological cell separation according to claim 1, wherein: the magnetism generated when the energizing coil (6) is energized is the same as the magnetism of one end of the decelerating magnet (4) close to the energizing coil (6).