CN112931219B - Tissue culture and rapid propagation method of Pandanus communis - Google Patents
Tissue culture and rapid propagation method of Pandanus communis Download PDFInfo
- Publication number
- CN112931219B CN112931219B CN202110388496.4A CN202110388496A CN112931219B CN 112931219 B CN112931219 B CN 112931219B CN 202110388496 A CN202110388496 A CN 202110388496A CN 112931219 B CN112931219 B CN 112931219B
- Authority
- CN
- China
- Prior art keywords
- culture
- culture medium
- induction
- seedlings
- pandanum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/22—Improving land use; Improving water use or availability; Controlling erosion
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention provides a tissue culture and rapid propagation method of Pandanum henryi, which comprises (1) taking lateral buds of 1-2 months old on a main stem of a Pandanum henryi stock plant as explants; (2) sterilizing, inoculating to an induction culture medium, performing dark culture, performing illumination culture, and performing synchronous induction and multiplication culture; the induction culture medium contains 0.5-0.6mg/L brassin; (3) when 1-1.5 cm of buds are differentiated, taking the buds, inoculating a new induction culture medium with the same formula for subculture; changing the induction culture medium every 5-8 days of culture, and increasing the brassin by 0.1-0.2 mg/L every time of change; (4) inoculating to a rooting culture medium for rooting culture to obtain Pandanus communis seedlings; (5) hardening, cleaning and transplanting the seedlings for cultivation; the invention can obviously improve the inductivity of the tissue culture and rapid propagation of the Pandanum henryi, effectively shorten the induced rooting period, and realize the rapid propagation of the Pandanum henryi tissue culture seedlings, wherein the seedling growth speed is high, and the root activity is good.
Description
Technical Field
The invention relates to the technical field of tissue culture, in particular to a tissue culture and rapid propagation method of Pandanum armatum.
Background
The Pandanus amabilis (Pandanus amarylifolius) is a plant spice, is called as a Pandanus amabilis leaf, resists yin and prefers moisture, rarely suffers from plant diseases and insect pests, and is a vanilla with strong vitality. It is rich in antioxidant active ingredients and unique fragrance, can be applied to various cakes, beverages and special meals, and has the effects of cooling, reducing internal heat, soothing nerves, calming, relaxing muscles and tendons and activating collaterals.
In recent years, the planting of the sachets sylvestris is gradually popularized in the fields of Hainan, Yunnan and the like, but the planting scale of the sachets sylvestris in Hainan is only about 2 ten thousand mu, the seedlings of the sachets sylvestris mainly adopt stem cutting propagation, the problems of difficult scale production, uneven individuals, disordered plant shapes after planting, low yield and the like exist in the breeding of the sachets sylvestris seedlings, so that the difficulty of breaking the tissue rapid propagation technology of the sachets sylvestris seedlings is urgently needed, and an important condition is provided for realizing that the planting industry of the sachets sylvestris in Hainan breaks through the scale of more than 100 ten thousand mu. However, the existing research on the tissue culture rapid propagation technology of the Pandanum henryi still has a big blank, and has the problems of low induction rate, low multiplication coefficient, poor root growth activity of tissue culture seedlings, long culture period and the like of the tissue culture rapid propagation of Pandanum henryi seedlings, so that the difficulty of popularization of the tissue culture rapid propagation technology of the Pandanum henryi is limited.
Disclosure of Invention
In view of this, the invention provides a method for tissue culture and rapid propagation of a Pandanus communis Hance.
The technical scheme of the invention is realized as follows:
the invention provides a tissue culture and rapid propagation method of Pandanum sachalinensis, which comprises the following steps:
step 1: cutting the newly-grown lateral buds on the main stem of the Pandanus communis stock plant to 1-2 cm to be used as explants;
step 2: sterilizing and disinfecting the explants, inoculating the explants to an induction culture medium, performing dark culture, then performing illumination culture, and performing synchronous induction and multiplication culture; the induction culture medium contains 0.5-0.6mg/L brassin;
and step 3: when the explants differentiate buds, taking the buds, and inoculating the buds to a new induction culture medium with the same formula as the step 2 for subculture; replacing a new induction culture medium every 5-8 days after culture, and increasing 0.1-0.2 mg/L of brassinolide in the induction culture medium replaced every time;
and 4, step 4: inoculating the cluster bud plants more than or equal to 2cm into a rooting culture medium for rooting culture until 9-12 cm-high Pandanum communis seedlings are obtained; returning the cluster bud plants with the length less than 2cm to the step 3 for subculture;
and 5: and (4) refining the Pandanum henryi seedlings obtained in the step (4), and then transplanting and cultivating the seedlings.
Further explaining, in the step 2, the explant is sterilized, specifically, the explant is soaked in 0.05-0.1% mercuric chloride solution in which 3-6 drops of tween-20 are dripped by a dripper with the diameter of 1.0mm for 10-15 min, is washed by sterile water for 2-3 times, is soaked in 75% ethanol solution for 30-60 s, is washed by the sterile water for 3-4 times, and is drained to reduce the explant-induced pollution.
More preferably, the induction medium is MS, 0.03-0.07mg/L TDZ, 0.8-1.0 mg/L6-BA, 0.5-0.6mg/L brassin, 0.2-0.8mg/L kinetin, 0.1-0.5mg/LNAA, 1.0-1.5g/L peptone, 8-12g/L white sugar and 4.5-6.0g/L agar;
the rooting culture medium comprises: 1/3MS, 0.8-1.5 mg/L6-BA, 0.05-0.1mg/L NAA, 0.02-0.04mg/L brassin, 5-7g/L banana puree, 10-20g/L white sugar and 7.0-8.0g/L agar.
More preferably, the induction medium is MS, 0.05mg/L TDZ, 0.9 mg/L6-BA, 0.55mg/L brassin, 0.5mg/L kinetin, 0.3mg/L LNAA, 1.3g/L peptone, 10g/L white sugar and 5.2g/L agar;
the rooting culture medium comprises: 1/3MS, 1.2 mg/L6-BA, 0.08mg/L NAA, 0.03mg/L brassin, 6g/L banana puree, 15g/L white sugar and 7.5g/L agar.
Further explaining, in the step 2, after the explants are inoculated to an induction culture medium, dark culture is carried out for 5-7 days at 25 +/-2 ℃; then, the temperature is increased to 26 +/-2 ℃, the illumination intensity is 1000-1500 lx, and the cultivation is carried out for 10-12 d under the condition that the illumination time is 3-5 h/d; and transferring the culture medium into a new induction culture medium with the same formula, and performing synchronous induction and multiplication culture under the conditions of 26 +/-2 ℃, the illumination intensity of 1500-2000 lx and the illumination time of 6-8 h/d.
More preferably, in the step 3, the temperature of the subculture is 28 +/-2 ℃, the illumination intensity is 1500-2000 lx, the illumination time is 10-12 h/d, and the subculture time is 20-24 d; regulating and controlling the condition of subculture, promoting the differentiation and proliferation of the adventitious bud, improving the proliferation coefficient of the adventitious bud and shortening the culture period.
More preferably, in the step 4, the temperature of rooting culture is 28 +/-2 ℃, the illumination intensity is 1500-2500 lux, the illumination time is 12-14 h/d, the rooting culture condition is regulated and controlled, the growth and development of the root system are promoted, and the induced rooting period is shortened.
More preferably, in the step 5, before hardening off seedlings, the Pandanus communis seedlings obtained in the step 4 are transferred to a solid MS culture medium for culture for 5-7 days, and the survival rate of transplanting the Pandanus communis seedlings is further improved.
More preferably, the refining of the seedlings of the Pandanum communis Linne is to transfer the Pandanum communis Linne seedlings with bottles to outdoor normal temperature to refine the seedlings for 5-7 d, and transplant the seedlings for cultivation after the seedlings are refined for 3-5 d after the cover is opened.
More preferably, in the step 2-4, the humidity of the synchronous induction and proliferation culture is 55-65%, and the humidity of the rooting culture is 60-80%.
Compared with the prior art, the invention has the beneficial effects that: the invention aims to fully improve the induction rate of the tissue culture and rapid propagation of the sacha rosea and effectively shorten the induced rooting period, the growth speed of the seedling is high, and the root activity of the tissue culture seedling is improved, thereby realizing the method for rapidly establishing a regeneration system of the tissue culture seedling of the sacha rosea in a short time, realizing the rapid propagation of the tissue culture seedling of the sacha rosea and providing important conditions for the supply and the propagation of the seedlings of the sacha rosea.
(1) According to the invention, the bud induction differentiation of the explant is directly promoted by effectively regulating the explant induction culture conditions, utilizing certain low-temperature dark culture and short-time illumination culture and combining with a corresponding induction culture medium formula, so that independent explant dedifferentiation is not required, the adventitious bud induction rate is improved, the adventitious bud character is ensured, synchronous bud induction differentiation and proliferation culture are realized, and the culture period is shortened.
(2) By selecting the buds with a certain differentiation degree and gradually adjusting and increasing the concentration of brassin in an induction culture medium in the process of subculture, the rapid growth of cluster bud plants is further promoted, and the multiplication coefficient of adventitious buds is remarkably improved while the stable growth quality of seedlings is ensured.
(3) By optimizing the rooting culture medium and adding a certain amount of banana puree by adopting a low-concentration brassin combination, the rooting of the seedlings of the Pandanus communis Hance is effectively promoted, the induced rooting is promoted, the root activity of the tissue culture seedlings of the Pandanus communis Hance is improved, and the survival of transplanting the seedlings of the Pandanus communis Hance is facilitated.
Detailed Description
In order that the technical contents of the invention may be better understood, specific examples are provided below to further illustrate the invention.
The experimental methods used in the examples of the present invention are all conventional methods unless otherwise specified.
The materials, reagents and the like used in the examples of the present invention are commercially available unless otherwise specified.
Example 1-tissue culture rapid propagation method of Pandanus communis seedlings, comprising the following steps:
(1) cutting the newly-born 1-month-old lateral bud on the main stem of the Pandanus communis stock plant to 2cm to be used as an explant;
(2) sterilizing and disinfecting the explant, inoculating the explant into an induction culture medium, and performing dark culture at 25 +/-2 ℃ for 5 days; culturing for 10 days at 26 + -2 deg.C and illumination intensity of 1000lx and illumination time of 3 h/d; transferring to a new induction culture medium with the same formula, and performing synchronous induction and multiplication culture at 26 +/-2 ℃, illumination intensity of 1500lx and illumination time of 6h/d, wherein the humidity is 55%;
the induction culture medium is MS, 0.03mg/L TDZ, 0.8 mg/L6-BA, 0.5mg/L brassin, 0.2mg/L kinetin, 0.1mg/L LNAA, 1.0g/L peptone, 8g/L white sugar and 4.5-6.0g/L agar;
(3) when 1cm of buds are differentiated from the explant, the buds are taken and inoculated into a new induction culture medium with the same formula as the step 2, and subcultured for 24d under the conditions that the temperature is 28 +/-2 ℃, the illumination intensity is 1500lx and the illumination time is 10h/d, and the humidity is 55%; changing a new induction culture medium every 8 days of culture, and increasing the brassin in the induction culture medium changed every time by 0.2 mg/L;
(4) inoculating the cluster bud plants more than or equal to 2cm into a rooting culture medium, and carrying out rooting culture under the conditions that the temperature is 28 +/-2 ℃, the illumination intensity is 1500lux, and the illumination time is 12h/d, wherein the humidity is 60 percent until the Pandanus communis seedlings with the height of 8cm are obtained; returning the cluster bud plants less than 2cm to the step 3 for subculture;
the rooting culture medium comprises: 1/3MS, 0.8 mg/L6-BA, 0.05mg/L NAA, 0.02mg/L brassin, 5g/L banana puree, 10g/L white sugar and 7.0g/L agar;
(5) and (4) transferring the Pandanum commune seedlings obtained in the step (4) to an outdoor room temperature with a bottle to harden the seedlings for 5d, uncovering and hardening the seedlings for 3d, taking out and cleaning the seedlings, and transplanting and cultivating the seedlings.
Wherein, the sterilization and disinfection of the explant are as follows: soaking the explant in 0.08% mercuric chloride solution dropwise added with 5 drops of Tween-20 for 13min, washing with sterile water for 3 times, soaking in 75% ethanol solution for 50s, washing with sterile water for 4 times, and draining.
Embodiment 2-tissue culture rapid propagation method of Pandanum Paniculatum seedlings, comprising the following steps:
(1) cutting the newly-born 2-month-old lateral buds on the main stem of the Pandanus communis stock plant to 1cm to be used as an explant;
(2) sterilizing and disinfecting the explant, inoculating the explant into an induction culture medium, and performing dark culture at 25 +/-2 ℃ for 7 days; rotating to 26 +/-2 ℃, culturing for 12d under the conditions of illumination intensity of 1500lx and illumination time of 5 h/d; transferring to a new induction culture medium with the same formula, and performing synchronous induction and proliferation culture under the conditions of 26 +/-2 ℃, illumination intensity of 2000lx and illumination time of 8h/d, wherein the humidity is 65%;
the induction culture medium is MS, 0.07mg/L TDZ, 1.0 mg/L6-BA, 0.6mg/L brassin, 0.8mg/L kinetin, 0.5mg/LNAA, 1.5g/L peptone, 12g/L white sugar and 6.0g/L agar;
(3) when 1.5cm of buds are differentiated from the explant, inoculating the buds into a new induction culture medium with the same formula as the step 2, and carrying out subculture for 20d under the conditions of the temperature of 28 +/-2 ℃, the illumination intensity of 2000lx and the illumination time of 12h/d, wherein the humidity is 65%; changing a new induction culture medium every 5 days of culture, and increasing the brassin in the induction culture medium changed every time by 0.1 mg/L;
(4) inoculating the cluster bud plants more than or equal to 2cm into a rooting culture medium, and carrying out rooting culture under the conditions that the temperature is 28 +/-2 ℃, the illumination intensity is 2500lux and the illumination time is 14h/d, wherein the humidity is 80 percent until the Pandanum cohnii seedlings with the height of 10cm are obtained; returning the cluster bud plants less than 2cm to the step 3 for subculture;
the rooting culture medium comprises: 1/3MS, 1.5 mg/L6-BA, 0.1mg/L NAA, 0.04mg/L brassin, 7g/L banana puree, 20g/L white sugar and 8.0g/L agar;
(5) and (4) transferring the Pandanum commune seedlings obtained in the step (4) to an outdoor room temperature with a bottle to harden the seedlings for 7d, uncovering and hardening the seedlings for 5d, taking out and cleaning the seedlings, and transplanting and cultivating the seedlings.
Embodiment 3-tissue culture rapid propagation method of Pandanus communis seedlings, comprising the following steps:
(1) cutting the newly-born 2-month-old lateral buds on the main stem of the Pandanus communis stock plant to 2cm to be used as explants;
(2) sterilizing and disinfecting the explant, inoculating the explant into an induction culture medium, and performing dark culture at 25 +/-2 ℃ for 6 days; rotating to 26 +/-2 ℃, and culturing for 11 days under the conditions of illumination intensity of 1500lx and illumination time of 4 h/d; transferring to a new induction culture medium with the same formula, and performing synchronous induction and proliferation culture under the conditions of 26 +/-2 ℃, illumination intensity of 2000lx and illumination time of 7h/d, wherein the humidity is 60%;
the induction culture medium is MS, 0.05mg/L TDZ, 0.9 mg/L6-BA, 0.55mg/L brassin, 0.5mg/L kinetin, 0.3mg/LNAA, 1.3g/L peptone, 10g/L white sugar and 5.2g/L agar;
(3) when 1.3cm of buds are differentiated from the explant, inoculating the buds into a new induction culture medium with the same formula as the step 2, and carrying out subculture for 23d under the conditions that the temperature is 28 +/-2 ℃, the illumination intensity is 2000lx and the illumination time is 11h/d, and the humidity is 60%; changing a new induction culture medium every 6 days of culture, and increasing the brassin in the induction culture medium changed every time by 0.15 mg/L;
(4) inoculating cluster bud plants more than or equal to 2cm into a rooting culture medium, and performing rooting culture at 28 +/-2 ℃, with illumination intensity of 2500lux and illumination time of 13h/d, wherein the humidity is 70% until Pandanus communis seedlings with the height of 9cm are obtained; returning the cluster bud plants less than 2cm to the step 3 for subculture;
the rooting culture medium comprises: 1/3MS, 1.2 mg/L6-BA, 0.08mg/L NAA, 0.03mg/L brassin, 6g/L banana puree, 15g/L white sugar and 7.5g/L agar;
(5) and (4) transferring the Pandanum commune seedlings obtained in the step (4) to an outdoor room temperature with a bottle to harden the seedlings for 6d, uncovering and hardening the seedlings for 4d, taking out and cleaning the seedlings, and transplanting and cultivating the seedlings.
Example 4-this example differs from example 3 in that: before hardening off seedlings, the obtained Pandanum cohnii seedlings are transferred to a solid MS culture medium, cultured for 6d and then transferred to the outdoor room temperature to be hardened off for 6 d.
Comparative example 1-this comparative example differs from example 3 in that: in the step 2, after the explant is inoculated to an induction culture medium, the explant is directly transferred to a new induction culture medium with the same formula after being cultured in dark at the temperature of 28 +/-2 ℃ for 17d, and the explant is synchronously induced and cultured in a proliferation way under the conditions of 26 +/-2 ℃, the illumination intensity of 2000lx and the illumination time of 7 h/d.
Comparative example 2-this comparative example differs from example 3 in that: the formula of the induction culture medium is adjusted as follows: 0.1mg/L, and the content of the brassin is unchanged in the process of subculture.
Comparative example 3-this comparative example differs from example 3 in that: the formula of the induction culture medium is adjusted as follows: 0.9mg/L, and in the process of subculture, the content of the brassinolide is increased by 0.1mg/L after replacing the induction culture medium every 6 days.
Comparative example 4-this comparative example differs from example 3 in that: the brassin content in the rooting medium formula is adjusted to be 0.06 mg/L.
Comparative example 5-this comparative example differs from example 3 in that: the formula of the rooting culture medium is adjusted to be not added with brassin, and the content of the banana puree is increased to 10 g/L.
And (3) determining the effect of tissue culture of the Pandanus communis Hance seedlings according to the tissue culture and rapid propagation method of the Pandanus communis Hance seedlings.
The experimental method comprises the following steps: taking the comparative examples in examples 1-3 and comparative examples 1-3 as treatment groups, each treatment group is repeated for 3 times, the blank control is culture by taking MS as a basic culture medium, and the bud induction rate, the proliferation times, the root induction period and the root growth conditions of the tissue culture seedlings of the Pandanum odoratum of each experimental group are respectively counted, and the results are shown in the following table:
the calculation method comprises the following steps: adventitious bud induction rate (%) × 100% (number of induced bud explants/number of inoculations); the multiplication times of the adventitious buds are equal to the multiplication adventitious buds number/inoculation buds number;
as can be seen from the above table, the tissue culture rapid propagation method of the chandanthus aromatica seedlings can realize the adventitious bud induction rate of more than 90%, and meanwhile, the multiplication times of the adventitious buds are obviously increased, while in comparative examples 1-3, the induction rate of the adventitious buds is reduced, the multiplication times are reduced, and meanwhile, the period from induction to rooting is obviously prolonged; the invention shows that the induction culture conditions of the explant are effectively controlled and the formula of the induction culture medium is optimized, so that the adventitious bud induction rate is effectively improved, the multiplication coefficient is obviously improved, and the culture period of the Pandanum paniculatum tissue culture seedling is shortened; in comparative examples 4-5, the induced rooting period is prolonged, the length of the adventitious roots is shortened, and the root activity is reduced, which shows that the method can promote induced rooting and improve the root activity of the tissue culture seedling of the Pandanum henryi by optimizing the rooting culture medium, adopting low-concentration brassin and adding a certain amount of banana mud.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and should not be taken as limiting the scope of the present invention, which is intended to cover any modifications, equivalents, improvements, etc. within the spirit and scope of the present invention.
Claims (9)
1. A tissue culture and rapid propagation method of Pandanum armatum Roxb is characterized in that: the method comprises the following steps:
step 1: cutting the newly-grown lateral buds on the main stem of the Pandanus communis stock plant to 1-2 cm to be used as explants;
and 2, step: sterilizing and disinfecting the explant, inoculating the explant into an induction culture medium, performing dark culture, then performing illumination culture, and performing synchronous induction and multiplication culture; the induction culture medium contains 0.5-0.6mg/L brassin;
and 3, step 3: when the explants differentiate buds, taking the buds, and inoculating the buds to a new induction culture medium with the same formula as the step 2 for subculture; replacing a new induction culture medium every 5-8 days after culture, and increasing 0.1-0.2 mg/L of brassinolide in the induction culture medium replaced every time;
and 4, step 4: inoculating cluster bud plants more than or equal to 2cm into a rooting culture medium for rooting culture until Pandanus communis seedlings with the height of 9-12 cm are obtained; returning the cluster bud plants less than 2cm to the step 3 for subculture;
and 5: refining the Pandanus communis Linne seedlings obtained in the step 4, and then transplanting and cultivating the seedlings;
the induction culture medium is MS, 0.03-0.07mg/L TDZ, 0.8-1.0 mg/L6-BA, 0.5-0.6mg/L brassin, 0.2-0.8mg/L kinetin, 0.1-0.5mg/LNAA, 1.0-1.5g/L peptone, 8-12g/L white sugar and 4.5-6.0g/L agar; the rooting culture medium comprises: 1/3MS, 0.8-1.5 mg/L6-BA, 0.05-0.1mg/L NAA, 0.02-0.04mg/L brassin, 5-7g/L banana puree, 10-20g/L white sugar and 7.0-8.0g/L agar.
2. The tissue culture and rapid propagation method of Pandanum commune according to claim 1, characterized in that: and 2, sterilizing and disinfecting the explant, specifically, dipping the explant in 0.05-0.1% mercuric chloride solution dropwise added with 3-6 drops of Tween-20 for 10-15 min, washing with sterile water for 2-3 times, dipping in 75% ethanol solution for 30-60 s, washing with sterile water for 3-4 times, and draining.
3. The tissue culture and rapid propagation method of Pandanum commune according to claim 1, characterized in that: the induction culture medium is MS, 0.05mg/L TDZ, 0.9 mg/L6-BA, 0.55mg/L brassin, 0.5mg/L kinetin, 0.3mg/LNAA, 1.3g/L peptone, 10g/L white sugar and 5.2g/L agar; the rooting culture medium comprises: 1/3MS, 1.2 mg/L6-BA, 0.08mg/L NAA, 0.03mg/L brassin, 6g/L banana puree, 15g/L white sugar and 7.5g/L agar.
4. The tissue culture and rapid propagation method of Pandanum commune according to claim 1, characterized in that: in the step 2, after the explant is inoculated to an induction culture medium, dark culture is carried out for 5-7 days at 25 +/-2 ℃; then, the temperature is increased to 26 +/-2 ℃, the illumination intensity is 1000-1500 lx, and the cultivation is carried out for 10-12 d under the condition that the illumination time is 3-5 h/d; and transferring the culture medium into a new induction culture medium with the same formula, and performing synchronous induction and multiplication culture under the conditions of 26 +/-2 ℃, the illumination intensity of 1500-2000 lx and the illumination time of 6-8 h/d.
5. The tissue culture and rapid propagation method of Pandanum commune according to claim 1, characterized in that: in the step 3, the temperature of the subculture is 28 +/-2 ℃, the illumination intensity is 1500-2000 lx, the illumination time is 10-12 h/d, and the subculture time is 20-24 d.
6. The tissue culture and rapid propagation method of Pandanum commune according to claim 1, characterized in that: in the step 4, the temperature of rooting culture is 28 +/-2 ℃, the illumination intensity is 1500-2500 lux, and the illumination time is 12-14 h/d.
7. The tissue culture and rapid propagation method of Pandanum commune according to claim 1, characterized in that: and (3) before hardening, transferring the Pandanum armatum seedlings obtained in the step (4) to a solid MS culture medium, and culturing for 5-7 days.
8. The tissue culture and rapid propagation method of the Pandanus communis according to claim 7, characterized in that: the refining of the seedlings of the Pandanus communis Lindl is realized by the steps of transferring the Pandanus communis Lindl seedlings to an outdoor normal temperature with a bottle to be refined for 5-7 d, uncovering and refining the seedlings for 3-5 d, and transplanting and cultivating the seedlings.
9. The tissue culture and rapid propagation method of Pandanum commune according to claim 1, characterized in that: in the step 2-4, the humidity of synchronous induction and proliferation culture is 55-65%, and the humidity of rooting culture is 60-80%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110388496.4A CN112931219B (en) | 2021-04-12 | 2021-04-12 | Tissue culture and rapid propagation method of Pandanus communis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110388496.4A CN112931219B (en) | 2021-04-12 | 2021-04-12 | Tissue culture and rapid propagation method of Pandanus communis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112931219A CN112931219A (en) | 2021-06-11 |
| CN112931219B true CN112931219B (en) | 2022-05-31 |
Family
ID=76231651
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110388496.4A Active CN112931219B (en) | 2021-04-12 | 2021-04-12 | Tissue culture and rapid propagation method of Pandanus communis |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112931219B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114097611B (en) * | 2021-10-29 | 2022-10-11 | 中国热带农业科学院橡胶研究所 | Low-cost method for breeding cymbidium maculatum tissue culture seedlings |
| CN115119747A (en) * | 2022-04-30 | 2022-09-30 | 中国热带农业科学院海口实验站 | Leaf tissue culture medium of cymbidium maculatum and tissue culture rapid propagation method |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6849453B2 (en) * | 2003-03-27 | 2005-02-01 | Council Of Scientific & Industrial Research | Method and composition for clonal propagation of Pandanus amaryllifolius |
| CN111226792B (en) * | 2020-02-18 | 2020-11-24 | 中国热带农业科学院香料饮料研究所 | High-throughput breeding method for leaf seedlings of cymbidium sinense |
-
2021
- 2021-04-12 CN CN202110388496.4A patent/CN112931219B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN112931219A (en) | 2021-06-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN112931219B (en) | Tissue culture and rapid propagation method of Pandanus communis | |
| CN111226792B (en) | High-throughput breeding method for leaf seedlings of cymbidium sinense | |
| CN102870683A (en) | Microbody propagation expanding method of aquilaria malaccensis | |
| CN112042537B (en) | Method for establishing bletilla striata plant regeneration system | |
| CN110199883B (en) | Cultivation method of panax notoginseng tissue culture seedlings | |
| CN116602213A (en) | Tissue culture method for one-step seedling formation of bulbil konjak leaf surface corm | |
| CN110839530A (en) | Method for inducing flowering of Chinese rose in test tube | |
| CN101536676B (en) | Simple and convenient transplantation method of rooting seedlings in blueberry tissue culture | |
| CN117158315A (en) | Tissue culture method of gardenia lobule | |
| CN107022519B (en) | Separation culture method of tea suspension single cells | |
| CN104855289A (en) | Method for culturing and producing micro-bulbodium of crocus sativus L. through superficial layer | |
| CN109479723A (en) | A method of improving Afriocan agapanthus body embryo seedling inducing effect | |
| CN112154919B (en) | Culture medium and method for inducing calli of paris polyphylla to directly grow seedlings | |
| CN110447538B (en) | Tissue culture method taking pennisetum panzeri inflorescence axes as explants | |
| CN104357374A (en) | Capsicum annuum L. anther tissue culture method | |
| CN108207624A (en) | A kind of red root wild silkworm beanstalk section tissue cultivating and seedling method | |
| CN107148912B (en) | A kind of quick breeding method for tissue culture of radix scutellariae | |
| CN116391620B (en) | Method for establishing efficient regeneration system by taking schizonepeta stems as explants | |
| CN117136842B (en) | Micro-tissue culture and rapid propagation method of cudrania tricuspidata | |
| CN103651119A (en) | Inducing method of gracilaria verrucosa callus | |
| CN111448988B (en) | Culture method and culture medium for inhibiting endophyte pollution in primary culture of magnolia zenii | |
| CN111802249B (en) | Method for decorating and transforming succulent plant of Crassulaceae | |
| AU2021102670A4 (en) | Method for Tissue Culture and Rapid Reproduction of Tall Fescue (Tall fescue) | |
| CN113826552B (en) | In-vitro rapid propagation method for seed cucurbita pepo | |
| CN109349103B (en) | Tissue culture and rapid propagation method for New Zealand spinach |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information |
Address after: 570100 Room 201, block a, Reke Plaza, No.4 Xueyuan Road, Longhua District, Haikou City, Hainan Province Applicant after: Hainan ReZuo High Tech Research Institute Co.,Ltd. Address before: 570100 Room 201, block a, Reke Plaza, No.4 Xueyuan Road, Longhua District, Haikou City, Hainan Province Applicant before: Hainan Rezuo Hi-Tech Research Institute Co.,Ltd. |
|
| CB02 | Change of applicant information | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |