CN112920252B

CN112920252B - Membrane separation equipment for polypeptide fractionation

Info

Publication number: CN112920252B
Application number: CN202110086239.5A
Authority: CN
Inventors: 周倩芸
Original assignee: Xiangya Biomedicine Huzhou Co ltd
Current assignee: Xiangya Biomedicine Huzhou Co ltd
Priority date: 2021-01-22
Filing date: 2021-01-22
Publication date: 2022-07-05
Anticipated expiration: 2041-01-22
Also published as: CN112920252A

Abstract

The invention discloses a membrane separation device for polypeptide fractionation, which structurally comprises a base, a machine body, a top cover and an input pipe, wherein the top surface of the base is welded and connected with the bottom surface of the machine body, and the bottom of the top cover is in transitional connection with the top of the machine body, in the process of separating macromolecular protein liquid, when a large amount of macromolecular protein is accumulated on a separation membrane to cause the trafficability of the macromolecular protein to be poor, a pressure plate is pressed downwards by the weight of the protein and the liquid plus the impact force generated when the liquid is sent downwards, a pressure air bag is scraped in the pressing-down process, air flow moves upwards to push an upper top head outwards, a throwing block is thrown out by the elastic force, an extending head stretches out by inertia to generate vibration simultaneously, a large amount of protein accumulated on the separation membrane is hammered to be scattered, and water molecules are scattered by the vibration to avoid the macromolecular protein accumulation from being too much to cause the trafficability to be reduced, the separation speed of the membrane separation equipment is ensured.

Description

Membrane separation equipment for polypeptide fractionation

Technical Field

The invention relates to the field of membrane separation equipment, in particular to membrane separation equipment for polypeptide fractionation.

Background

The membrane is a material with selective separation function, the separation and purification work of different components of raw material liquid can be realized through the different selective separation functions of different membranes, the process of separating through the membrane is called membrane separation, the membrane can separate the liquid containing protein molecules, the protein molecules can be effectively separated from dissolved liquid, and the protein is made to adhere to the membrane, so that collection can be conveniently carried out, but when the membrane separation equipment separates the macromolecular protein for a long time, one surface of the membrane is easily adhered to a large amount of the macromolecular protein, the pore diameter through which the water molecules can pass on the surface of the membrane is made to be blocked in a large amount, and the trafficability of the surface of the membrane to the water molecules is greatly reduced, so that the liquid flowing speed is greatly reduced, and the separation speed of the membrane separation equipment is influenced.

Disclosure of Invention

In view of the above problems, the present invention provides a membrane separation apparatus for fractionation of polypeptides.

In order to achieve the purpose, the invention is realized by the following technical scheme: a membrane separation device for polypeptide fractionation structurally comprises a base, a machine body, a top cover and an input pipe, wherein the top surface of the base is welded with the bottom surface of the machine body, the bottom of the top cover is in transitional connection with the top of the machine body, and the bottom surface of the input pipe is communicated with the top surface of the top cover and is connected with the top surface of the top cover through electric welding; the organism includes outer wall, isolating construction, direction slope, excretion pipe, the outer wall inlayer is connected with the outer loop of isolating construction inlays admittedly, direction slope bottom surface welds in the outer wall inlayer, excretion pipe right side and outer wall left side switch-on each other and through the electric welding connection, isolating construction is equipped with threely, and three isolating construction clearance distributes in the outer wall inlayer uniformly.

Furthermore, the separation structure comprises a separation film, a lower pressing strip, a substrate and an upper ejection structure, wherein the bottom surface of the separation film is fixedly connected with the top surface of the lower pressing strip, the top surface of the upper ejection structure is fixedly connected with the bottom surface of the lower pressing strip, the bottom of the upper ejection structure is embedded into the top surface of the substrate, the number of the upper ejection structures is eight, and the eight upper ejection structure gaps are uniformly distributed on the top surface of the substrate.

Further, go up the top structure and include clamp plate, vibrations groove, pressure spring, go up the kicking block, the clamp plate is inside to be integrated into one piece with the vibrations groove, the pressure spring top is connected with clamp plate bottom surface is inlayed admittedly, go up kicking block left side and clamp plate right side transitional coupling, the vibrations groove is equipped with threely, and three vibrations groove clearance distributes inside the clamp plate evenly.

Furthermore, the upper ejector block comprises an installation block, an air flow channel, air pressing bags, a pull-back groove and an upper ejector head, the interior of the installation block and the air flow channel are integrally formed, the air pressing bags are embedded in the left side of the installation block, the pull-back groove and the top surface of the installation block are integrally formed, the bottom of the upper ejector head is in contact with the inner layer of the pull-back groove, the number of the air pressing bags is two, and the two air pressing bags are distributed on two sides of the installation block in a mirror image mode.

Further, go up the top including getting rid of piece, bottom plate, outer lane shell, fixed axle, dragging the strip, get rid of a bottom surface and be connected with outer lane shell skin is inlayed admittedly, outer lane shell inlayer is connected with the fixed axle outer loop through dragging the strip, the fixed axle both ends are inlayed admittedly in the bottom plate top surface, it is equipped with five to drag the strip, and five are dragged the strip and are star-shaped distribution between fixed axle and outer lane shell.

Furthermore, the throwing block comprises a connecting sleeve, a bearing block, a groove plate and four extending heads, the outer layer of the connecting sleeve is fixedly connected with the bottom of the bearing block, the bottom surface of the groove plate is fixedly connected with the top surface of the bearing block, the extending heads are embedded into the top surface of the groove plate, and the four extending heads are uniformly distributed on the outer layer of the groove plate in a ring shape in the gap.

Furthermore, stretch out the head including back extension spring, attaching plate, spliced pole, stretch out ring, inertia hammer, back extension spring top surface and attaching plate bottom surface welded connection, attaching plate top surface through the spliced pole with stretch out the ring outer loop and be connected, inertia hammer bottom activity block in stretching out the ring inner ring, the inertia hammer is equipped with eight, eight inertia hammer clearance evenly ring form distribute in stretching out the ring inlayer.

Advantageous effects

Compared with the prior art, the invention has the following beneficial effects:

in the process of separating the macromolecular protein liquid, when a large amount of macromolecular protein is accumulated on the separation membrane to cause the passing property of the macromolecular protein to be poor, the pressure plate can be pressed downwards by the weight of the protein and the liquid plus the impact force generated when the liquid is sent downwards, the pressure plate is pressed downwards, the pressure air bag is scraped in the pressing process, the air flow moves upwards to push the upper ejector head outwards, the throwing block is thrown out by the elastic force, the extending head extends out by inertia and generates vibration at the same time, the protein accumulated on the separation membrane in large amount is hammered to be scattered, and the protein is scattered by the vibration, so that the phenomenon that the passing property of water molecules is reduced due to excessive accumulation of the macromolecular protein is avoided, and the separation speed of the membrane separation equipment is ensured.

Drawings

FIG. 1 is a schematic perspective view of a membrane separation apparatus for fractionation of a polypeptide of the present invention.

Fig. 2 is a schematic structural view of a front section of the machine body of the present invention.

Fig. 3 is a schematic structural view of a front cross section of the separation structure of the present invention.

Fig. 4 is a schematic structural view of a front section of the roof structure of the present invention.

Fig. 5 is a schematic structural view of a front cross section of the upper top block of the present invention.

Fig. 6 is a schematic structural view of a front cross section of the upper plug according to the present invention.

Fig. 7 is a schematic structural view of a front section of the throwing block of the present invention.

Fig. 8 is a schematic structural view of a front cross section of the extension head of the present invention.

In the figure: base-1, machine body-2, top cover-3, input tube-4, outer wall-21, separation structure-22, guide slope-23, drain tube-24, separation membrane-221, lower pressing strip-222, base plate-223, upper top structure-224, pressing plate-A1, vibration groove-A2, pressure spring-A3, upper top block-A4, mounting block-A41, air flow channel-A42, air pressing bag-A43, pull-back groove-A44, upper top head-A45, swinging block-B1, bottom plate-B2, outer ring shell-B3, fixing shaft-B4, pull strip-B5, connecting sleeve-B11, bearing block-B12, groove plate-B13, extension head-B14, pull-C1, attachment plate-C2, connecting column-C3, extension ring-C4, Inertial hammer-C5.

Detailed Description

The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and it is obvious that the described embodiments are some, not all embodiments of the present invention, and all other embodiments obtained by those skilled in the art without creative efforts based on the embodiments of the present invention belong to the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

The first embodiment is as follows:

referring to fig. 1-8, the embodiments of the present invention are as follows: a membrane separation device for polypeptide fractionation structurally comprises a base 1, a machine body 2, a top cover 3 and an input pipe 4, wherein the top surface of the base 1 is connected with the bottom surface of the machine body 2 in a welding mode, the bottom of the top cover 3 is connected with the top of the machine body 2 in a transition mode, and the bottom surface of the input pipe 4 is communicated with the top surface of the top cover 3 and is connected with the top surface of the top cover 3 through electric welding; organism 2 includes outer wall 21, isolating construction 22, direction slope 23,

excretes pipe

24, 21 inlayers of outer wall are connected with the outer loop of isolating construction 22 is embedded solid, the 23 bottom surfaces of direction slope weld in 21 inlayers of outer wall, it connects and through the electric welding to excrete pipe 24 right side and 21 left sides of outer wall switch-on each other, isolating construction 22 is equipped with threely, and three isolating construction 22 clearance distributes in 21 inlayers of outer wall evenly, is favorable to carrying out the fractionation to polypeptide protein through multilayer isolating construction.

The separation structure 22 includes a separation film 221, a lower pressing bar 222, a substrate 223, and an upper top structure 224, wherein the bottom surface of the separation film 221 is fixedly connected to the top surface of the lower pressing bar 222, the top surface of the upper top structure 224 is fixedly connected to the bottom surface of the lower pressing bar 222, the bottom of the upper top structure 224 is embedded into the top surface of the substrate 223, the number of the upper top structures 224 is eight, and the eight upper top structures 224 are uniformly distributed on the top surface of the substrate 223 in a clearance manner, which is beneficial to shaking the separation film in all directions.

Wherein, it includes clamp plate A1, vibrations groove A2, pressure spring A3, goes up top piece A4 to go up top structure 224, the inside shaping as an organic whole with vibrations groove A2 of clamp plate A1, pressure spring A3 top and clamp plate A1 bottom surface are inlayed and are connected admittedly, go up top piece A4 left side and clamp plate A1 right side transitional coupling, vibrations groove A2 is equipped with threely, and three vibrations groove A2 clearance distributes uniformly inside clamp plate A1, is favorable to increasing the vibrations intensity when pushing down and kick-backing.

The upper ejector block A4 comprises an installation block A41, an air flow channel A42, an air pressing bag A43, a pull-back groove A44 and an upper ejector head A45, wherein the interior of the installation block A41 and the air flow channel A42 are integrally formed, the air pressing bag A43 is embedded into the left side of the installation block A41, the pull-back groove A44 and the top surface of the installation block A41 are integrally formed, the bottom of the upper ejector head A45 is in contact with the inner layer of the pull-back groove A44, two air pressing bags A43 are arranged, and the two air pressing bags A43 are distributed on the two sides of the installation block A41 in a mirror image mode, so that air pressure during compression can be enhanced.

The upper ejector head A45 comprises a throwing block B1, a bottom plate B2, an outer ring shell B3, a fixed shaft B4 and pulling strips B5, the bottom surface of the throwing block B1 is fixedly embedded with the outer layer of an outer ring shell B3, the inner layer of the outer ring shell B3 is connected with the outer ring of the fixed shaft B4 through the pulling strips B5, the two ends of the fixed shaft B4 are fixedly embedded on the top surface of the bottom plate B2, the pulling strips B5 are five, the five pulling strips B5 are distributed between the fixed shaft B4 and the outer ring shell B3 in a star shape, and the throwing speed of the throwing block B1 is higher due to the fact that throwing force is increased.

Based on the above embodiment, the specific working principle is as follows: liquid to be separated is added into the machine body 2 from the input pipe 4, and is enabled to freely fall into the separation structure 22 in the machine body 2 through gravity, the liquid falls on the separation structure 22, so that the separation membrane 221 on the top surface of the separation structure 22 is impacted and pressed downwards, the pressing strip 222 is further pressed downwards, the pressing plate A1 of the upper top structure 224 is enabled to move downwards, the pressing spring A3 is pressed, meanwhile, pressure is generated on the side edge of the upper top block A4, certain vibration is generated through the vibration groove A3 during pressing downwards, the vibration is transmitted to the separation membrane 221, a part of protein sediment is scattered, meanwhile, the side edge of the upper top head A45 is pressed, the pressing air bag A43 is pressed to generate air, the air bag is pressed into the air flow channel A42 to be blown upwards, the upper top head A45 is enabled to move upwards through air pressure, the pulling back to the groove A44 is pulled back, after the upper top head A45 moves upwards, and the block B1 loses the blocking of the pressing plate A1, the pulling strip B5 is caused to recover rapidly, the outer ring shell B3 is twisted to cause the throwing block B1 to throw away rapidly, and the descending separation membrane 221 is hammered to break up the accumulated macromolecular proteins on the upper surface of the separation membrane 221.

Example two:

referring to fig. 6-8, the embodiment of the present invention is as follows: the swinging block B1 comprises a connecting sleeve B11, a bearing block B12, a groove plate B13 and an extending head B14, the outer layer of the connecting sleeve B11 is fixedly connected with the bottom of the bearing block B12 in an embedded manner, the bottom surface of the groove plate B13 is fixedly connected with the top surface of the bearing block B12 in an embedded manner, the extending head B14 is embedded into the top surface of the groove plate B13, the extending heads B14 are four, and the four extending heads B14 are uniformly and annularly distributed on the outer layer of the groove plate B13 in a gap manner, so that the increase of the range and strength of extending hammering is facilitated.

Wherein, it includes back extension spring C1, rigging board C2, spliced pole C3, stretches out ring C4, inertia hammer C5 to stretch out head B14, it is connected with rigging board C2 bottom surface welded to return extension spring C1 top surface, rigging board C2 top surface is through spliced pole C3 with stretch out ring C4 outer loop connection, inertia hammer C5 bottom activity block in stretching out ring C4 inner ring, inertia hammer C5 is equipped with eight, and eight inertia hammer C5 intervals evenly cyclic annular distribution in stretching out ring C4 inlayer are favorable to producing stronger continuous vibrations through inertia, shakes the attached object and scatters.

Based on the above embodiment, the specific working principle is as follows: when the swinging block B1 swings outwards, the extension head B14 stays in place due to inertia and generates relative displacement with the groove plate B13 of the swinging block B1 to press the tension spring C1 back, after the swinging block B1 stops moving, due to the combined action of the inertia and the tension spring C1, the extension ring C4 is attached to the groove plate B13 at the attachment plate C2 to move outwards, so that the extension ring C4 extends, the membrane structure is hammered again, and the membrane structure is hammered again and stopped when hammered, so that the inner inertia hammer C5 hammered the inner layer of the extension ring C4 again through inertia swinging, and the large-molecule protein on the upper surface of the membrane is shaken out again.

The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Claims

1. The utility model provides a membrane separation equipment of polypeptide fractionation which the structure includes base (1), organism (2), top cap (3), input tube (4), its characterized in that:

the top surface of the base (1) is welded with the bottom surface of the machine body (2), the bottom of the top cover (3) is in transitional connection with the top of the machine body (2), and the bottom surface of the input pipe (4) is communicated with the top surface of the top cover (3) and is connected with the top surface of the top cover (3) through electric welding;

the machine body (2) comprises an outer wall (21), a separation structure (22), a guide slope (23) and a drainage pipe (24), wherein the inner layer of the outer wall (21) is fixedly connected with the outer ring of the separation structure (22), the bottom surface of the guide slope (23) is welded on the inner layer of the outer wall (21), and the right side of the drainage pipe (24) is communicated with the left side of the outer wall (21) and is connected with the left side of the outer wall (21) through electric welding;

the separation structure (22) comprises a separation film (221), a lower pressing strip (222), a substrate (223) and an upper top structure (224), wherein the bottom surface of the separation film (221) is fixedly connected with the top surface of the lower pressing strip (222), the top surface of the upper top structure (224) is fixedly connected with the bottom surface of the lower pressing strip (222), and the bottom of the upper top structure (224) is embedded in the top surface of the substrate (223);

go up top structure (224) including clamp plate (A1), vibrations groove (A2), pressure spring (A3), go up top piece (A4), inside and vibrations groove (A2) integrated into one piece of clamp plate (A1), pressure spring (A3) top and clamp plate (A1) bottom surface are embedded solid and are connected, go up top piece (A4) left side and clamp plate (A1) right side transitional coupling.

2. A membrane separation device for polypeptide fractionation according to claim 1, wherein: the upper top block (A4) comprises a mounting block (A41), an air flow channel (A42), an air pressing bag (A43), a pull-back groove (A44) and an upper top head (A45), wherein the inside of the mounting block (A41) and the air flow channel (A42) are integrally formed, the air pressing bag (A43) is embedded into the left side of the mounting block (A41), the pull-back groove (A44) and the top surface of the mounting block (A41) are integrally formed, and the bottom of the upper top head (A45) and the inner layer of the pull-back groove (A44) are in contact with each other.

3. A membrane separation device for polypeptide fractionation according to claim 2, wherein: go up top (A45) including getting rid of piece (B1), bottom plate (B2), outer lane shell (B3), fixed axle (B4), pull strip (B5), get rid of piece (B1) bottom surface and outer embedded solid connection of outer lane shell (B3), outer lane shell (B3) inlayer is connected with fixed axle (B4) outer loop through pulling strip (B5), fixed axle (B4) both ends are embedded solid in bottom plate (B2) top surface.

4. A membrane separation device for polypeptide fractionation according to claim 3, wherein: the swinging block (B1) comprises a connecting sleeve (B11), a bearing block (B12), a groove plate (B13) and an extending head (B14), wherein the outer layer of the connecting sleeve (B11) is fixedly embedded with the bottom of the bearing block (B12), the bottom surface of the groove plate (B13) is fixedly embedded with the top surface of the bearing block (B12), and the extending head (B14) is embedded into the top surface of the groove plate (B13).

5. A membrane separation device for polypeptide fractionation according to claim 4, wherein: stretch out head (B14) including returning extension spring (C1), rigging board (C2), spliced pole (C3), stretch out ring (C4), inertia hammer (C5), it is connected with rigging board (C2) bottom surface welded to return extension spring (C1) top surface, rigging board (C2) top surface is through spliced pole (C3) and stretch out ring (C4) outer loop connection, inertia hammer (C5) bottom activity block is in stretching out ring (C4) inner ring.