CN112898970A - Preparation and application for detecting carbon spots of progesterone - Google Patents
Preparation and application for detecting carbon spots of progesterone Download PDFInfo
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- CN112898970A CN112898970A CN202110158022.0A CN202110158022A CN112898970A CN 112898970 A CN112898970 A CN 112898970A CN 202110158022 A CN202110158022 A CN 202110158022A CN 112898970 A CN112898970 A CN 112898970A
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- RJKFOVLPORLFTN-LEKSSAKUSA-N Progesterone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H](C(=O)C)[C@@]1(C)CC2 RJKFOVLPORLFTN-LEKSSAKUSA-N 0.000 title claims abstract description 97
- 239000000186 progesterone Substances 0.000 title claims abstract description 48
- 229960003387 progesterone Drugs 0.000 title claims abstract description 48
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 title claims abstract description 36
- 229910052799 carbon Inorganic materials 0.000 title claims abstract description 36
- 238000002360 preparation method Methods 0.000 title claims abstract description 12
- VFLDPWHFBUODDF-FCXRPNKRSA-N curcumin Chemical compound C1=C(O)C(OC)=CC(\C=C\C(=O)CC(=O)\C=C\C=2C=C(OC)C(O)=CC=2)=C1 VFLDPWHFBUODDF-FCXRPNKRSA-N 0.000 claims abstract description 20
- 238000000034 method Methods 0.000 claims abstract description 18
- 239000000126 substance Substances 0.000 claims abstract description 16
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 claims abstract description 15
- 229940109262 curcumin Drugs 0.000 claims abstract description 10
- 235000012754 curcumin Nutrition 0.000 claims abstract description 10
- 239000004148 curcumin Substances 0.000 claims abstract description 10
- VFLDPWHFBUODDF-UHFFFAOYSA-N diferuloylmethane Natural products C1=C(O)C(OC)=CC(C=CC(=O)CC(=O)C=CC=2C=C(OC)C(O)=CC=2)=C1 VFLDPWHFBUODDF-UHFFFAOYSA-N 0.000 claims abstract description 10
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 8
- 238000000502 dialysis Methods 0.000 claims abstract description 5
- 238000004108 freeze drying Methods 0.000 claims abstract description 4
- 238000010438 heat treatment Methods 0.000 claims abstract description 4
- 239000012265 solid product Substances 0.000 claims abstract description 4
- 239000002904 solvent Substances 0.000 claims abstract description 4
- 238000005303 weighing Methods 0.000 claims abstract description 4
- 239000000243 solution Substances 0.000 claims description 16
- 238000003556 assay Methods 0.000 claims description 4
- 238000001514 detection method Methods 0.000 claims description 4
- 230000005284 excitation Effects 0.000 claims description 4
- 239000011259 mixed solution Substances 0.000 claims description 3
- 238000012544 monitoring process Methods 0.000 abstract description 5
- 230000035945 sensitivity Effects 0.000 abstract description 5
- 238000001917 fluorescence detection Methods 0.000 abstract description 4
- 239000002086 nanomaterial Substances 0.000 abstract description 2
- 230000003542 behavioural effect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 206010049872 Breast discomfort Diseases 0.000 description 1
- 208000005623 Carcinogenesis Diseases 0.000 description 1
- 206010012374 Depressed mood Diseases 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000006399 behavior Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008195 breast development Effects 0.000 description 1
- 230000036952 cancer formation Effects 0.000 description 1
- 231100000504 carcinogenesis Toxicity 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000002848 electrochemical method Methods 0.000 description 1
- 230000013020 embryo development Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 210000001672 ovary Anatomy 0.000 description 1
- 230000027758 ovulation cycle Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 230000035935 pregnancy Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000010206 sensitivity analysis Methods 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000003270 steroid hormone Substances 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
- C09K11/08—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials
- C09K11/65—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials containing carbon
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y20/00—Nanooptics, e.g. quantum optics or photonic crystals
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y40/00—Manufacture or treatment of nanostructures
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- C—CHEMISTRY; METALLURGY
- C01—INORGANIC CHEMISTRY
- C01B—NON-METALLIC ELEMENTS; COMPOUNDS THEREOF; METALLOIDS OR COMPOUNDS THEREOF NOT COVERED BY SUBCLASS C01C
- C01B32/00—Carbon; Compounds thereof
- C01B32/15—Nano-sized carbon materials
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
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Abstract
A preparation method and application for detecting carbon points of progesterone belong to the technical field of nano materials, and comprise the following steps of firstly, weighing 0.1-2 g of curcumin, putting the curcumin into a beaker, and heating the curcumin in a muffle furnace for 6 hours at the temperature of 170-190 ℃ to obtain brown substances; step two, after the brown substance obtained in the step one is naturally cooled to room temperature, dissolving the brown substance in an ethanol solvent, centrifuging the brown substance at 10000rpm for 30 minutes, and dialyzing the brown substance by using a dialysis bag to remove unreacted micromolecules to obtain a carbon dot solution; and step three, preparing the carbon dot solution obtained in the step two into solid product carbon dot CDs by adopting a freeze drying method. The method has the advantages of high efficiency and sensitivity of a fluorescence detection method, good stability of carbon dots and high luminous intensity, and has wide application prospect in the field of progesterone specificity monitoring.
Description
Technical Field
The invention belongs to the technical field of nano materials, and particularly relates to an application study for detecting progesterone based on fluorescent carbon dots.
Background
Progesterone (P4) is a steroid hormone secreted by the ovary, plays a crucial role in maintaining and regulating the physiological processes of female menstrual cycle, embryonic development, pregnancy and breast development, and is also widely used in human medicine and clinical treatment. However, abuse of progesterone can cause undesirable side effects such as weakness, mood depression, breast discomfort, certain potential carcinogenesis, and the like. The sensitivity analysis of progesterone monitoring in clinical medicine, animal husbandry and environmental protection is highly required. Therefore, the method has important significance for efficiently and sensitively detecting the progesterone.
Methods for detecting progesterone include chromatographic methods, colorimetric methods, electrochemical methods, etc., however, these methods suffer from certain disadvantages, such as long sample pretreatment time, expensive reagents required, long run time, and low sensitivity. The fluorescence detection method has the advantages of simple operation, high sensitivity and the like.
Disclosure of Invention
The technical problem to be solved by the invention is as follows: the preparation and application for detecting the progesterone carbon dots have the advantages of high efficiency, sensitivity, good carbon dot stability and high luminous intensity of a fluorescence detection method, and have wide application prospects in the field of progesterone specificity monitoring.
A preparation for detecting carbon spots of progesterone is characterized by comprising the following components in parts by weight: comprises the following steps which are sequentially carried out,
step one, weighing 0.1g to 2g of curcumin, putting the curcumin into a beaker, and heating the curcumin in a muffle furnace for 6 hours at the temperature of between 170 and 190 ℃ to obtain a brown substance;
step two, after the brown substance obtained in the step one is naturally cooled to room temperature, dissolving the brown substance in an ethanol solvent, centrifuging the brown substance at 10000rpm for 30 minutes, and dialyzing the brown substance by using a dialysis bag to remove unreacted micromolecules to obtain a carbon dot solution;
step three, preparing the carbon dot solution obtained in the step two into solid product carbon dot CDs by adopting a freeze drying method;
to this end, a preparation for detecting carbon spots of progesterone was completed.
Use for detecting the carbon spot of progesterone, characterized by: a progesterone assay using the progesterone-containing carbon dots for progesterone assay prepared by the method of claim 1, wherein the progesterone solution is first added to 1mL of carbon-dot CDs solution with a concentration of 0.2mg/mL, from low to high, and left to stand for 1 minute; then, the excitation wavelength was set at 410nm, and the fluorescence intensity of the mixed solution was measured, and the fluorescence intensity of the carbon dots was gradually increased as the concentration of progesterone was increased.
The concentration of the progesterone solution can be selected from 0,10,20,30,40,50,60,70,80,90,100,110,120 and 130 micromolar.
Through the design scheme, the invention can bring the following beneficial effects: the preparation and the application for detecting the carbon dots of the progesterone have the advantages of high efficiency and sensitivity of a fluorescence detection method, good stability of the carbon dots and high luminous intensity, and have wide application prospect in the field of progesterone specificity monitoring;
furthermore, the carbon dots act on a fluorescence sensing platform, and have good analysis and monitoring capability on various ions, amino acids and the like.
Drawings
The invention is further described with reference to the following figures and detailed description:
FIG. 1 is a schematic diagram of the optical properties of prepared and applied carbon dots CDs for detecting progesterone carbon dots according to the present invention.
Fig. 2 is a behavioral diagram a of the preparation and application of the fluorescent carbon spot-based rapid progesterone detection for detecting the carbon spot of progesterone in accordance with the present invention.
FIG. 3 is a behavioral graph B of a method for detecting carbon spots of progesterone according to the present invention and using fluorescent carbon spots to rapidly detect progesterone.
Detailed Description
A method for detecting carbon point of progesterone comprises weighing 0.5g (allowable range of 0.1-2 g) curcumin, placing into a beaker, and heating in a muffle furnace at 180 deg.C (allowable range of 170-190 deg.C) for 6 hr to obtain brown substance. After the reaction kettle is naturally cooled to room temperature, the mixture is dissolved in an ethanol solvent at 10000rpm and centrifuged for 30 minutes, and then a dialysis bag is used for dialysis to remove unreacted small molecules. Finally, the solid product carbon points CDs are obtained by a freeze drying method.
The carbon dots CDs prepared by the preparation method have the following optical properties:
as shown in FIG. 1, the maximum excitation wavelength of the carbon dots is 410nm, and the maximum emission peak is 560 nm.
The fluorescent carbon spot CDs prepared by the preparation method are used for detecting progesterone as follows,
first, progesterone solutions of various concentrations (0,10,20,30,40,50,60,70,80,90,100,110,120, 130 micromolar) were added to 1mL of carbon dot (0.2mg/mL) solution and allowed to stand for 1 minute. Then, the excitation wavelength was set at 410nm, and the mixed solution was tested for fluorescence intensity value.
In order to verify the stability of the carbon dot CDs, the fluorescence response behaviors of the carbon dot solution and other interferent solutions are researched by a similar method under the condition of room temperature; the corresponding ion-selective experimental procedure was the same as above, with an interferent (Cu)2+,Fe3+,K+,Na+,Pb2+,Hg2+,Al3+,Mn2+,Ca2+,AA, Cys，H2O2At a concentration of 120 micromolar) instead of the corresponding progesterone.
As shown in fig. 2 and 3, the fluorescence intensity of the carbon spot gradually increased with the increase in the concentration of progesterone. Meanwhile, the figure shows that a good linear relation exists between the fluorescence intensity of the carbon spot and the concentration of the progesterone, and the detection limit of the carbon spot to the progesterone is calculated to be 9.3 nM. Meanwhile, in the presence of the corresponding interferents, only progesterone has a remarkable enhancement effect on the fluorescence of the carbon dot solution, and other interferents have no remarkable influence on the fluorescence intensity of the carbon dot solution.
Therefore, the fluorescent carbon dots prepared by the method can be applied to the aspects of detecting the progesterone specifically and sensitively by utilizing the property of the fluorescent carbon dots for enhancing the fluorescence of the progesterone, and can provide a sensitive and accurate detection result.
Claims (3)
1. A preparation for detecting carbon spots of progesterone is characterized by comprising the following components in parts by weight: comprises the following steps which are sequentially carried out,
step one, weighing 0.1g to 2g of curcumin, putting the curcumin into a beaker, and heating the curcumin in a muffle furnace for 6 hours at the temperature of between 170 and 190 ℃ to obtain a brown substance;
step two, after the brown substance obtained in the step one is naturally cooled to room temperature, dissolving the brown substance in an ethanol solvent, centrifuging the brown substance at 10000rpm for 30 minutes, and dialyzing the brown substance by using a dialysis bag to remove unreacted micromolecules to obtain a carbon dot solution;
step three, preparing the carbon dot solution obtained in the step two into solid product carbon dot CDs by adopting a freeze drying method;
to this end, a preparation for detecting carbon spots of progesterone was completed.
2. Use for detecting the carbon spot of progesterone, characterized by: a progesterone assay using the progesterone-containing carbon dots for progesterone assay prepared by the method of claim 1, wherein the progesterone solution is first added to 1mL of carbon-dot CDs solution with a concentration of 0.2mg/mL, from low to high, and left to stand for 1 minute; then, the excitation wavelength was set at 410nm, and the mixed solution was tested for fluorescence intensity value, and the fluorescence intensity of the carbon spot gradually increased as the concentration of progesterone increased.
3. The use according to claim 1 for the detection of the carbon spot of progesterone, wherein: the concentration of the progesterone solution can be selected from 0,10,20,30,40,50,60,70,80,90,100,110,120 and 130 micromolar.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110158022.0A CN112898970A (en) | 2021-02-05 | 2021-02-05 | Preparation and application for detecting carbon spots of progesterone |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202110158022.0A CN112898970A (en) | 2021-02-05 | 2021-02-05 | Preparation and application for detecting carbon spots of progesterone |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113025319A (en) * | 2021-03-08 | 2021-06-25 | 中国科学院苏州生物医学工程技术研究所 | Carbon point for detecting progesterone and progesterone detection method |
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2021
- 2021-02-05 CN CN202110158022.0A patent/CN112898970A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113025319A (en) * | 2021-03-08 | 2021-06-25 | 中国科学院苏州生物医学工程技术研究所 | Carbon point for detecting progesterone and progesterone detection method |
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Application publication date: 20210604 |