CN112898591B - Carrier for compound microbial preparation and preparation method thereof - Google Patents

Carrier for compound microbial preparation and preparation method thereof Download PDF

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CN112898591B
CN112898591B CN202110188841.XA CN202110188841A CN112898591B CN 112898591 B CN112898591 B CN 112898591B CN 202110188841 A CN202110188841 A CN 202110188841A CN 112898591 B CN112898591 B CN 112898591B
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modified gelatin
carrier
gelatin
solution
spiraea
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CN112898591A (en
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徐坚麟
付源
王俊滔
向粤琴
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Hangzhou Nanda Environmental Protection Technology Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08H—DERIVATIVES OF NATURAL MACROMOLECULAR COMPOUNDS
    • C08H1/00—Macromolecular products derived from proteins
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier

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Abstract

The invention discloses a carrier for a compound microbial preparation and a preparation method thereof, wherein the preparation method comprises the following steps: dissolving modified gelatin in deionized water at 65-85 ℃ in a water bath environment until the modified gelatin is completely dissolved to obtain a modified gelatin solution; adding diatomite, sodium malate and calcium chloride into the modified gelatin solution, completely mixing, and cooling to 5-15 ℃; wherein the modified gelatin is prepared from spiraea glycoside modified gelatin. The carrier for the compound microorganism preparation has higher mechanical strength, excellent enzymolysis resistance and permeability, is combined with the compound microorganism, is applied to sewage treatment, has higher COD removal rate, can be widely applied to the fields of industrial wastewater, domestic sewage, water body restoration and agricultural production, and is safe and nontoxic.

Description

Carrier for compound microbial preparation and preparation method thereof
Technical Field
The invention belongs to the technical field of microorganism application, and particularly relates to a carrier for a compound microbial preparation and a preparation method thereof.
Background
For a long time, the treatment of urban sewage aims at removing organic matters and suspended solids, and does not consider the removal of organic nutrients such as ammonia nitrogen and the like. With the increasing total amount of sewage discharge and the wide application of chemical fertilizers, synthetic detergents and pesticides, the influence of nitrogen nutrients in wastewater on the environment is more and more paid attention by people. The most prominent influence of nitrogen on the water environment is eutrophication of water bodies, particularly closed water bodies, which is represented by excessive propagation of algae and subsequent deterioration of water quality to lake degradation; secondly, the oxygen consumption characteristic of ammonia nitrogen can reduce the dissolved oxygen of the water body, thereby leading to fish death and black and odorous water body. In the water treatment of the closed circulation system, the immobilized microorganisms can be used for effectively removing nitrogen, phosphorus, COD and enriched heavy metals in the water body, thereby obtaining the effect of purifying the water quality.
At present, researchers research microbial carriers, such as whisk researches a novel basalt fiber biological carrier (refer to environmental engineering, 2019,37(09):1-7+39), and as BF is regarded more seriously in the environmental protection field, the hydraulic characteristics, the specific surface area, the biological affinity and the like of the BF meet the requirements of the water quality purification biological carrier; therefore, the Basalt Fiber (BF) as a novel microbial carrier has good biocompatibility and water quality purification effect, and the novel BF biological carrier material, the water quality purification mechanism and the application of water treatment are discussed; the BF hydrophilic material is modified to improve the hydrophilicity, weak electropositivity, roughness and the like of the surface of the BF, and improve the biological affinity of the BF and the dispersibility of the BF in water. Von Benxiu (refer to Guangdong university of industry 2006) researches that the polyvinyl alcohol is used as a skeleton carrier, the activated carbon is used as an adsorbent, an embedding method is adopted to fix nitrobacteria, and a new additive is used as an auxiliary carrier to overcome the defects of easy breakage, easy adhesion, low strength and the like in the process of embedding the polyvinyl alcohol.
Disclosure of Invention
The invention aims to provide modified gelatin with a better net structure and higher strength.
The technical scheme adopted by the invention for realizing the purpose is as follows:
a modified gelatin is prepared from spiraea glycoside modified gelatin.
The hydratin modified gelatin is adopted, so that a good network structure is formed inside the gelatin, the mechanical strength of the gelatin is improved, and the gelatin has good stability; the reason may be that hydroxyl and aldehyde groups in the spiraea glycoside and active groups in the gelatin are subjected to cross-linking reaction to form a good network structure, so that the mechanical strength of the gelatin is improved, the gelatin has good stability, and the gelatin can be widely applied to microbial carriers.
Preferably, the modified gelatin is prepared as follows:
dissolving 5-15 parts by weight of gelatin in a phosphate buffer solution, completely dissolving in a constant-temperature water bath kettle at 55-65 ℃, then slowly dropwise adding 0.008-0.03 part of an spiraea glycosides solution, cooling to room temperature after reaction, dropwise adding into 20-30 parts of cold butyl acetate which is continuously stirred, controlling the size of the beads by adjusting the rotating speed to enable the size of the beads to be 2-4 mm, soaking the obtained beads in 80-100 mL of the spiraea glycosides solution, storing in a refrigerator at 2-5 ℃ for 15-30 min, washing with water for three times, draining to obtain modified gelatin, and placing the modified gelatin in the refrigerator for later use.
More preferably, the concentration of the spiraea glycoside solution is 0.2-0.8 wt%.
The invention also discloses the application of the spiraea glycoside in improving the mechanical strength of the gelatin.
The invention also discloses the use of the modified gelatin in a carrier for microorganisms.
The invention also discloses application of the modified gelatin in improving the enzymolysis resistance of the carrier for the microorganisms.
The invention also discloses a carrier for the compound microorganism, which is prepared from the modified gelatin.
Preferably, the composite microorganism supports a weight of more than 530 g.
Another object of the present invention is to provide a carrier for complex microorganisms, which has high mechanical strength and good enzymatic hydrolysis resistance and permeability, and can be stably combined with complex microorganisms, and thus can be widely used in sewage treatment.
The invention also discloses a preparation method of the carrier for the compound microorganism.
The technical scheme adopted by the invention for realizing the purpose is as follows:
a preparation method of a carrier for a compound microorganism comprises the following steps:
dissolving modified gelatin in deionized water at 65-85 ℃ in a water bath environment until the modified gelatin is completely dissolved to obtain a modified gelatin solution;
adding diatomite, sodium malate and calcium chloride into the modified gelatin solution, completely mixing, and cooling to 5-15 ℃.
The carrier for the compound microorganism is prepared by compounding the modified gelatin, the diatomite, the sodium malate and the calcium chloride, and has high mechanical strength and good enzymolysis resistance; the reasons are that the modified gelatin interacts with each component, the internal structure and the performance of the modified gelatin are improved, the modified gelatin has higher mechanical strength, and simultaneously the carrier for the compound microorganism has better enzymolysis resistance, namely better stability, and can resist the rupture caused by the propagation of the microorganism and have long service life by combining the carrier with the compound microorganism; the composite microorganism is solidified on the carrier, the combination of the composite microorganism and the carrier has better stability, the formed microbial preparation has higher removal rate to COD in sewage treatment, can be widely applied to the fields of industrial wastewater, domestic sewage, water body restoration and agricultural production, and is safe and nontoxic.
Preferably, the concentration of the modified gelatin solution is 10-30 wt%.
Preferably, the modified gelatin solution comprises, by weight, 45-60 parts of diatomite, 0.5-1.5 parts of sodium malate, 0.15-0.65 parts of calcium chloride and 0.06-0.14 parts of calcium chloride.
Preferably, the microorganism immobilized with the carrier for complex microorganism includes anaerobic bacteria and/or aerobic bacteria.
The invention also discloses application of the carrier for the compound microorganism in the fields of treating industrial wastewater, domestic sewage, water body restoration and/or agricultural production.
The invention also discloses the application of the mixture of cucurbitacin and acesulfame potassium in improving the permeability of the carrier for the compound microorganism.
In order to further improve the mechanical strength of the complex microbial carrier and the stability of the complex microbial carrier combined with the complex microbial carrier, and simultaneously ensure that the complex microbial carrier has good permeability, the preferable measures further comprise:
in the process of preparing the carrier for the microorganism, 1.2-2.7 parts by weight of a mixture of cucurbitacin and acesulfame potassium is added, wherein the weight ratio of the added cucurbitacin to the acesulfame potassium is 0.01-0.06: 1, so that the mixture is compounded with modified gelatin, diatomite, sodium malate and calcium chloride to obtain the carrier for the composite microorganism, the mechanical strength of the carrier and the stability of combination with the composite microorganism are further improved, and the carrier has good permeability and enzymolysis resistance; the reason may be that the mixture of cucurbitacin and acesulfame potassium is added to interact with the modified gelatin and each component, so that the internal structure or the surface appearance of the modified gelatin is changed, the performance of the modified gelatin is further improved, and the carrier for the composite microorganism with better mechanical strength and composite microorganism combination is obtained.
The invention adopts the modified gelatin, the diatomite, the sodium malate and the calcium chloride to prepare the composite carrier for the microorganism in a composite way, thereby having the following beneficial effects: the carrier for the compound microorganism has higher mechanical strength and better enzymolysis resistance; the reasons are that the modified gelatin interacts with each component, the internal structure of the modified gelatin is improved, the carrier has higher mechanical strength, and meanwhile, the carrier for the compound microorganism has better enzymolysis resistance, namely better stability, and can resist the rupture caused by the propagation of the microorganism and have long service life by combining the carrier with the compound microorganism; the composite microorganism is solidified on the carrier, the combination of the two has better stability, and the formed microbial preparation has higher removal rate to COD in sewage treatment. Therefore, the carrier for the composite microorganism has higher mechanical strength, excellent enzymolysis resistance and permeability, is combined with the composite microorganism, is applied to sewage treatment, has higher COD removal rate, can be widely applied to the fields of industrial wastewater, domestic sewage, water body restoration and agricultural production, and is safe and nontoxic.
Drawings
FIG. 1 is an SEM image of the internal structure of unmodified gelatin;
FIG. 2 SEM image of the internal structure of modified gelatin;
FIG. 3 is a graph showing the strength of a carrier for complex microorganisms;
FIG. 4 is a light transmittance of a carrier for complex microorganisms;
FIG. 5 is a graph showing the degree of hydrolysis of a carrier for complex microorganisms;
FIG. 6 shows the removal rate of COD in wastewater treated with the composite microorganism in which the composite microorganism is supported by the carrier.
Detailed Description
The technical solution of the present invention is further described in detail below with reference to the following detailed description and the accompanying drawings:
in the embodiment of the invention, the preparation method of the modified gelatin is as follows:
dissolving 12 parts by weight of gelatin in a phosphate buffer solution, completely dissolving the gelatin in a constant-temperature water bath kettle at 60 ℃, then slowly dropwise adding 0.01 part by weight of an spiraea glycosides solution with the concentration of 0.8 wt%, cooling to room temperature after reaction, dropwise adding the mixture into 25 parts by weight of cold butyl acetate which is continuously stirred, controlling the size of the beads by adjusting the rotating speed to enable the size of the beads to be 3mm, soaking the obtained beads in 85mL of the spiraea glycosides solution, storing the beads in a refrigerator at 3 ℃ for 20min, washing with water for three times, draining to obtain modified gelatin, and placing the modified gelatin in the refrigerator for later use.
Example 1
A method for preparing a carrier for a complex microorganism, comprising the steps of:
dissolving modified gelatin in deionized water at 75 ℃ in a water bath environment until the modified gelatin is completely dissolved to obtain a modified gelatin solution, wherein the concentration of the modified gelatin solution is 18.8 wt%;
0.85 weight part of diatomite, 0.35 weight part of sodium malate and 0.12 weight part of calcium chloride are added into 55 weight parts of modified gelatin solution, completely mixed and cooled to 10 ℃ to obtain the carrier for the compound microorganism.
Example 2
A method for preparing a composite microbial carrier, which comprises the following steps in the same manner as example 1, except that:
adding 1.2 parts by weight of diatomite, 0.5 part by weight of sodium malate and 0.09 part by weight of calcium chloride into 48 parts by weight of the modified gelatin solution, completely mixing, and cooling to 10 ℃ to obtain the carrier for the composite microorganism.
Example 3
A method for preparing a composite microbial carrier, which comprises the following steps in the same manner as example 1, except that:
adding 0.85 part by weight of diatomite, 0.35 part by weight of sodium malate, 0.12 part by weight of calcium chloride, and 1.2 parts by weight of a mixture of cucurbitacin and acesulfame potassium into 55 parts by weight of the modified gelatin solution, wherein the weight ratio of the cucurbitacin to the acesulfame potassium is 0.1:1, completely mixing the mixture, and cooling to 10 ℃ to obtain the carrier for the composite microorganism.
Comparative example 1
A method for preparing a composite microbial carrier, which comprises the following steps in the same manner as example 1, except that: the modified gelatin solution was replaced with a gelatin solution.
Comparative example 2
A method for preparing a composite microbial carrier, which comprises the following steps in the same manner as in example 3, except that: adding 0.85 weight part of diatomite, 0.35 weight part of sodium malate, 0.12 weight part of calcium chloride and 1.2 weight parts of cucurbitacin into 55 weight parts of modified gelatin solution, completely mixing, and cooling to 10 ℃ to obtain the carrier for the composite microorganism.
Comparative example 3
A method for preparing a carrier for a complex microorganism, which comprises the same steps as those in example 3 except that: the difference from example 3 is: adding 0.85 weight part of diatomite, 0.35 weight part of sodium malate, 0.12 weight part of calcium chloride and 1.2 weight parts of potassium acetylsulfanilate into 55 weight parts of modified gelatin solution, completely mixing, and cooling to 10 ℃ to obtain the carrier for the composite microorganism.
Test example 1:
1. determination of surface morphology of modified gelatin
In this test, the surface morphology of the gelatin samples before and after modification was measured by a QUANTA250 type scanning electron microscope (FEI Co.).
Fig. 1 is an SEM image of the internal structure of unmodified gelatin. As can be seen from figure 1, the internal structure of the unmodified gelatin is loose, holes and cracks are formed, the structure is irregular and disordered, and the pore size distribution is not uniform; FIG. 2 SEM image of the internal structure of modified gelatin; as can be seen from fig. 2, the gelatin modified by the spiraea glycosides has a good network structure, a compact internal structure, a small and uniform pore diameter, and a tighter pore connection, which may be caused by a cross-linking reaction between hydroxyl groups and aldehyde groups in the spiraea glycosides and active groups in the gelatin to form a good network structure, thereby improving the mechanical strength of the gelatin and providing the modified gelatin with good stability.
Test example 2
1. Determination of Carrier Strength for Complex microorganisms
Placing a plurality of prepared carriers for the composite microorganisms into a square on a horizontal glass surface, placing a glass dish on a small ball, slowly adding the mass of the code-removing and code-removing on the glass dish from small to large, carefully observing by naked eyes until the carriers deform, and calculating the maximum mass which can be borne by the carriers;
the weight borne by each carrier (weight of glass dish + weight)/5
FIG. 3 shows the strength of the composite microorganism carrier. As can be seen from FIG. 3, the weight borne by the composite microorganism carrier in examples 1-2 was higher than 530g, the weight borne by the carrier in comparative example 1 and comparative example 1 was higher than that in comparative example 1, which indicates that the strength of the composite microorganism carrier was improved by using the modified gelatin modified with spiraeoside in the carrier; the weight of the carrier for the composite microorganism in example 3 is more than 600g, the carrier in example 1 is compared with that in example 3, and the weight of the carrier in example 3 is more than that in example 1 and that in comparative examples 2 to 3, which shows that the strength of the carrier for the composite microorganism is further improved by adding the mixture of cucurbitacin and acesulfame potassium in the carrier.
2. Determination of the Permeability of Complex microorganisms with Carrier
And placing the prepared experimental sample into the quantitatively diluted ink, keeping the temperature for 3h, taking out, and scanning and testing the light transmittance of the ink solution in the wave band of 450-700nm by adopting a Japanese U3010 type ultraviolet-visible spectrophotometer.
FIG. 4 shows the light transmittance of the composite microorganism carrier. In fig. 4, curve 1 is the light transmittance of the support in example 3, curve 2 is the light transmittance of the support in example 1, curve 3 is the light transmittance of the support in comparative example 3, curve 4 is the light transmittance of the support in comparative example 2, and curve 5 is the light transmittance of the support in comparative example 1; as can be seen from fig. 4, the ultraviolet transmittance at the wavelength band of 450-700nm of example 3 is higher than that of example 1, comparative example 2 and comparative example 3, which shows that the transmittance of the composite microbial carrier is further improved by simultaneously adding the mixture of cucurbitacin and acesulfame potassium in the carrier, i.e. the composite microbial carrier has better permeability; comparing example 1 with comparative example 1, the light transmittance of example 1 is higher than that of comparative example 1, which shows that the modified gelatin modified by spiraeoside is used in a carrier to improve the permeability of the carrier for complex microorganisms.
3. Determination of enzymatic hydrolysis resistance of composite microorganism carrier
Adding a phosphate buffer solution (pH is 7.0) which is 20 times of the weight of the carrier sample into a triangular flask, adding papain of which the weight is 3% of the weight of the carrier sample, adding the carrier sample, shaking up, placing in a constant-temperature oscillator, keeping the temperature of a water bath at 40 ℃, taking a hydrolysate after 2h, inactivating the enzyme in the boiling water bath for 5min, measuring the content of amino nitrogen in the hydrolysate by an ninhydrin method, and measuring the content of total nitrogen by a Kjeldahl method. The degree of hydrolysis DH (%) is calculated as follows:
DH(％)＝(Ah-A0)/(Az-A0)×100％
in the formula: a. theh-content of amino nitrogen (μ g) in the hydrolysate at different times; a. the0-the inherent amino nitrogen content in gelatin (. mu.g); a. thez-total nitrogen content of gelatin (. mu.g).
FIG. 5 shows the degree of hydrolysis of the composite microorganism carrier. As can be seen from fig. 5, the hydrolysis degree of examples 1-2 is lower than 6.5%, the hydrolysis degree of comparative example 1 and comparative example 1 is much lower than that of comparative example 1, which indicates that the hydrolysis degree of the carrier for the composite microorganism is improved by using the modified gelatin modified by the spiraea glycoside as the carrier for the composite microorganism, probably because the hydroxyl group and the aldehyde group in the spiraea glycoside and the active group in the gelatin are subjected to a cross-linking reaction to form a good network structure, thereby enhancing the enzymolysis resistance of the carrier for the composite microorganism and enabling the carrier for the composite microorganism to have good stability; the hydrolysis degree of the carriers for the compound microorganisms is not obviously different from that of the carriers for the comparative examples 1, 3 and 2-3, and the hydrolysis degree of the carriers for the compound microorganisms is hardly influenced by adding the mixture of the cucurbitacin and the acesulfame potassium in the carriers and adding the cucurbitacin and the acesulfame potassium alone, namely, the enzymolysis resistance of the carriers for the compound microorganisms is not influenced.
4. Determination of carrier-supported microorganism treated sewage for compound microorganism
The sewage of the test is taken from a Hangzhou city sewage plant, self-made compound microorganisms are added into a dissolved carrier aqueous solution according to an embedding ratio of 2:1, the mixture is uniformly stirred and immobilized, then the mixture is used for sewage treatment, a potassium dichromate method is adopted to determine the COD value in the sewage, the concentration of COD in the original sewage is 275mg/L, and the treatment effect is measured according to the removal rate of the COD of the sewage.
FIG. 6 shows the COD removal rate of wastewater treated with the composite microorganism-supporting microorganism. As can be seen from FIG. 6, the COD removal rates in the sewage gradually increased with the treatment time of examples 1-3 and comparative examples 2-3; comparing example 1 with example 3 and comparative examples 2-3, the COD removal rate of example 3 is higher than that of example 1 and comparative examples 2-3, which shows that the simultaneous addition of the mixture of cucurbitacin and acesulfame potassium in the carrier improves the removal rate of COD in sewage by the carrier loaded with the composite microorganisms, so that the carrier can be stably combined with the composite microorganisms to achieve the effect of effectively treating sewage; as can be seen from fig. 6, the removal rate of COD in sewage in comparative example 1 tends to increase and then decrease with the increase of treatment time, and the removal rate of COD in sewage in comparative example 1 is better than that in comparative example 1, which shows that the modified gelatin obtained by modifying gelatin with spiraea glycosides is used as a carrier for composite microorganisms, so that the removal rate of COD in sewage is improved, and the gelatin has better stability and can be used in sewage treatment for a long time to realize ecological restoration.
Conventional operations in the operation steps of the present invention are well known to those skilled in the art and will not be described herein.
The above embodiments are merely illustrative, and not restrictive, and those skilled in the art can make various changes and modifications without departing from the spirit and scope of the invention. Therefore, all equivalent technical solutions also belong to the scope of the present invention, and the protection scope of the present invention should be defined by the claims.

Claims (10)

1. A modified gelatin is prepared from spiraeoside modified gelatin;
dissolving 5-15 parts by weight of gelatin in a phosphate buffer solution, completely dissolving the gelatin in a constant-temperature water bath kettle at 55-65 ℃, then slowly dropwise adding 0.008-0.03 part of an spiraea glycoside solution, cooling to room temperature after reaction, dropwise adding the mixture into 20-30 parts of cold butyl acetate which is continuously stirred, controlling the size of the beads by adjusting the rotating speed to enable the size of the beads to be 2-4 mm, soaking the obtained beads in 80-100 mL of the spiraea glycoside solution, storing the beads in a refrigerator at 2-5 ℃ for 15-30 min, washing with water for three times, draining to obtain modified gelatin, and placing the modified gelatin in the refrigerator for later use;
the concentration of the spiraea glycoside solution is 0.2-0.8 wt%.
2. Use of the modified gelatin of claim 1 in a carrier for microorganisms.
3. Use of the modified gelatin of claim 1 to increase the resistance of a carrier for microorganisms to enzymatic hydrolysis.
4. The application of the spiraea glycoside in improving the mechanical strength of the gelatin is characterized in that: dissolving 5-15 parts by weight of gelatin in a phosphate buffer solution, completely dissolving the gelatin in a constant-temperature water bath kettle at 55-65 ℃, then slowly dropwise adding 0.008-0.03 part of an spiraea glycoside solution, cooling to room temperature after reaction, dropwise adding the mixture into 20-30 parts of cold butyl acetate which is continuously stirred, controlling the size of the beads by adjusting the rotating speed to enable the size of the beads to be 2-4 mm, soaking the obtained beads in 80-100 mL of the spiraea glycoside solution, storing the beads in a refrigerator at 2-5 ℃ for 15-30 min, washing with water for three times, draining to obtain modified gelatin, and placing the modified gelatin in the refrigerator for later use;
the concentration of the spiraea glycoside solution is 0.2-0.8 wt%.
5. A carrier for a complex microorganism, which is prepared from the modified gelatin of claim 1.
6. The carrier for a complex microorganism according to claim 5, wherein: the composite microorganism carrier can bear a weight of more than 530 g.
7. The method for preparing a complex microorganism carrier according to claim 5, comprising the steps of:
dissolving modified gelatin in deionized water at 65-85 ℃ in a water bath environment until the modified gelatin is completely dissolved to obtain a modified gelatin solution;
adding diatomite, sodium malate and calcium chloride into the modified gelatin solution, completely mixing, and cooling to 5-15 ℃.
8. The method for producing a composite microorganism carrier according to claim 7, wherein: the modified gelatin solution comprises, by weight, 25-45 parts of modified gelatin solution, 0.5-1.5 parts of diatomite, 0.15-0.65 parts of sodium malate and 0.06-0.14 parts of calcium chloride.
9. Use of the composite microorganism carrier according to claim 5 in the fields of treatment of industrial wastewater, domestic sewage, water remediation and/or agricultural production.
10. Use of a mixture of cucurbitacin and acesulfame potassium for increasing the permeability of a complex microbial carrier according to claim 5.
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DE102004024635A1 (en) * 2004-05-12 2005-12-08 Deutsche Gelatine-Fabriken Stoess Ag Process for the preparation of moldings based on crosslinked gelatin
JPWO2008072379A1 (en) * 2006-12-13 2010-03-25 富士フイルム株式会社 Method for producing modified biopolymer and method for cross-linking biopolymer
CN102924727B (en) * 2012-06-28 2014-12-10 中国科学院化学研究所 Aldehyde group modified gelatin material and preparation method thereof
CN103319729B (en) * 2013-06-04 2015-10-28 武汉工程大学 A kind of method that function is gelatin modified
CN108159502A (en) * 2018-03-06 2018-06-15 广州中医药大学第附属医院 Aurantiin microballoon fibroin albumen/hydroxyapatite compound rest and preparation method thereof

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