CN112896768B - Rapid DNA detection kit and preparation method thereof - Google Patents
Rapid DNA detection kit and preparation method thereof Download PDFInfo
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- CN112896768B CN112896768B CN202110053864.XA CN202110053864A CN112896768B CN 112896768 B CN112896768 B CN 112896768B CN 202110053864 A CN202110053864 A CN 202110053864A CN 112896768 B CN112896768 B CN 112896768B
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- 238000001514 detection method Methods 0.000 title claims abstract description 13
- 108091036333 Rapid DNA Proteins 0.000 title claims abstract description 11
- 238000002360 preparation method Methods 0.000 title abstract description 7
- 238000012360 testing method Methods 0.000 claims abstract description 49
- 108020004414 DNA Proteins 0.000 claims abstract description 5
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 claims description 30
- 239000003153 chemical reaction reagent Substances 0.000 claims description 21
- 239000002502 liposome Substances 0.000 claims description 18
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims description 15
- KWIUHFFTVRNATP-UHFFFAOYSA-N Betaine Natural products C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 claims description 15
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 15
- KWIUHFFTVRNATP-UHFFFAOYSA-O N,N,N-trimethylglycinium Chemical compound C[N+](C)(C)CC(O)=O KWIUHFFTVRNATP-UHFFFAOYSA-O 0.000 claims description 15
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims description 15
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 15
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 15
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 15
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 15
- 229960003237 betaine Drugs 0.000 claims description 15
- 239000001110 calcium chloride Substances 0.000 claims description 15
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 15
- 239000008367 deionised water Substances 0.000 claims description 15
- 229910021641 deionized water Inorganic materials 0.000 claims description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims description 12
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 9
- XKRFYHLGVUSROY-UHFFFAOYSA-N Argon Chemical compound [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 claims description 6
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- 229910052786 argon Inorganic materials 0.000 claims description 3
- 238000000861 blow drying Methods 0.000 claims description 3
- 238000001704 evaporation Methods 0.000 claims description 3
- 239000012528 membrane Substances 0.000 claims description 3
- 238000000034 method Methods 0.000 claims description 3
- 229910052757 nitrogen Inorganic materials 0.000 claims description 3
- 239000008055 phosphate buffer solution Substances 0.000 claims description 3
- 239000002096 quantum dot Substances 0.000 claims description 3
- 239000002994 raw material Substances 0.000 claims description 3
- 230000000694 effects Effects 0.000 description 3
- 230000009471 action Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000001427 coherent effect Effects 0.000 description 2
- 230000000670 limiting effect Effects 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 230000004308 accommodation Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D25/00—Details of other kinds or types of rigid or semi-rigid containers
- B65D25/02—Internal fittings
- B65D25/10—Devices to locate articles in containers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D25/00—Details of other kinds or types of rigid or semi-rigid containers
- B65D25/02—Internal fittings
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D25/00—Details of other kinds or types of rigid or semi-rigid containers
- B65D25/20—External fittings
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D25/00—Details of other kinds or types of rigid or semi-rigid containers
- B65D25/38—Devices for discharging contents
- B65D25/52—Devices for discharging successive articles or portions of contents
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
- B65D81/02—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents specially adapted to protect contents from mechanical damage
- B65D81/05—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents specially adapted to protect contents from mechanical damage maintaining contents at spaced relation from package walls, or from other contents
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- Engineering & Computer Science (AREA)
- Mechanical Engineering (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a rapid DNA detection kit and a preparation method thereof, and particularly relates to the technical field of DNA detection. According to the invention, by arranging the handle, the connecting shaft, the first piston, the connecting rod, the second piston and the movable assembly, the device can jack up a row of test tubes by adopting air pressure push type operation without manually taking the test tubes one by one, so that the test tubes in the jack-up state are easier to observe, the time is saved, and the problem of confusing and placing the test tubes is avoided, therefore, the device has good use value, and the practicability of the device is improved.
Description
Technical Field
The invention relates to the technical field of DNA detection, in particular to a rapid DNA detection kit and a preparation method thereof.
Background
At the in-process that detects DNA, often need use the kit, for convenient to detect a plurality of samples simultaneously, general kit all sets up for rectangular array, when needs observe the test tube of inside, often need manually take out a plurality of test tube respectively and watch, and is comparatively troublesome, not only causes the confusion easily, can waste the longer time moreover to be not convenient for to the observation of test tube in the kit, need improve.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention provides a rapid DNA detection kit and a preparation method thereof, and the invention aims to solve the technical problems that: general kit all is the setting of rectangle array, when needs observe the test tube of inside, often needs manually to take out a plurality of test tube respectively and watches, and is comparatively troublesome, not only causes the confusion easily, can waste the longer time moreover to the problem of the observation of test tube in the kit of not being convenient for.
In order to achieve the purpose, the invention provides the following technical scheme: the utility model provides a quick DNA detect reagent box, is including placing the board, the upper surface of placing the board has seted up a plurality of recess, the spout has all been seted up to the left surface of recess inner wall and the right flank of inner wall, sliding connection has the slider in the spout, two the opposite face of slider respectively with the left and right sides face fixed connection of frame, the internal surface of frame is provided with a plurality of test tube, the upper surface of test tube is provided with the stopcock, be provided with reagent in the test tube, the through-hole has been seted up to the upper surface of placing the board, the institute the left surface of the inner wall of through-hole is linked together with the right flank of a plurality of recess inner wall.
The improved piston type air compressor is characterized in that a cylinder body is arranged in the through hole, a first piston is arranged on the inner surface of the cylinder body, a bearing is connected to the upper surface of the first piston in a clamping mode, a connecting shaft is sleeved on the inner surface of the bearing, a second piston is arranged on the inner surface of the cylinder body, the left side surface of the second piston is fixedly connected with the right end of the connecting rod, the upper surface of the connecting rod is fixedly connected with the lower surface of the movable assembly, and the lower surface of the movable assembly is fixedly connected with the upper surface of the cylinder body.
As a further scheme of the invention: the upper surface of spout inner wall and the one end fixed connection of first spring, the other end of first spring and the upper surface fixed connection of slider, the lower surface of first piston and the one end fixed connection of second spring, the other end of second spring and the lower fixed connection of barrel inner wall.
As a further scheme of the invention: the left surface of through-hole inner wall and the right flank of inner wall have all seted up the guide way, the internal surface sliding connection of guide way has the guide block, two the opposite face of guide block respectively with the left surface and the right flank fixed connection of barrel.
As a further scheme of the invention: the outer surface of the connecting shaft is fixedly connected with the inner surface of the baffle, the top end of the connecting shaft is fixedly connected with the lower surface of the handle, a placing hole is formed in the upper surface of the barrel, and the connecting shaft is located in the placing hole.
As a further scheme of the invention: the movable component comprises two fixed blocks, the back surfaces of the two fixed blocks are fixedly connected with the lower surface of the movable plate and the upper surface of the barrel respectively, the front surfaces of the two fixed blocks are movably connected with the back surfaces of the two movable rods respectively through first pin shafts, the opposite surfaces of the two movable rods are movably connected through fourth pin shafts, the back surface of the front movable rod is movably connected with the front surface of the connecting block through a third pin shaft, the lower surface of the connecting block is fixedly connected with the upper surface of the connecting rod, the back surface of the rear movable rod is movably connected with the front surface of the supporting block through a second pin shaft, a movable sleeve is clamped on the left side surface of the supporting block, a supporting rod is sleeved on the inner surface of the movable sleeve, and the left end and the right end of the supporting rod are fixedly connected with the left side surface of the inner wall of the fixed groove and the right side surface of the inner wall respectively, the fixed groove is formed in the lower surface of the movable plate.
A preparation method of a rapid DNA detection kit reagent comprises the following steps:
s1, a rapid DNA detection kit, which comprises liposome, betaine, ammonium sulfate, trehalose, calcium chloride, sodium azide, TritonX-1000 and deionized water, wherein the mass ratio of the liposome, the betaine, the ammonium sulfate, the trehalose, the calcium chloride, the sodium azide, the TritonX-1000 and the deionized water is 12:4:5:3:2:1: 73.
S2, adding raw materials of liposome and quantum dots into a reactor, adding chloroform, uniformly mixing at 35-45 ℃, evaporating chloroform, blow-drying with nitrogen or argon, adding phosphate buffer solution, ultrasonically oscillating for 4-6 min, extruding and filtering for multiple times by using a microporous filtering membrane to obtain a liposome mixture, adjusting the pH value of betaine, ammonium sulfate, trehalose, calcium chloride, sodium azide, TritonX-1000 and deionized water to 8.8 by using sodium hydroxide, and uniformly mixing the obtained liposome mixture, betaine, ammonium sulfate, trehalose, calcium chloride, sodium azide, TritonX-1000 and deionized water, liposome, betaine, ammonium sulfate, trehalose, calcium chloride, sodium azide, TritonX-1000 and deionized water to obtain a reagent.
The invention has the beneficial effects that:
1. according to the test tube lifting device, the handle, the connecting shaft, the first piston, the connecting rod, the second piston and the movable assembly are arranged, the connecting shaft drives the first piston to move upwards under the pulling of the handle, the second piston drives the connecting rod to move rightwards, and the movable assembly can be controlled to upwards push the frame to drive the test tubes to move upwards to work, so that the test tube lifting device can jack up a row of test tubes by adopting air pressure push type operation without manually taking the test tubes one by one, the test tubes in a jack-up state are easier to observe, the time is saved, the problem of confusing and placing the test tubes is avoided, the device has good use value, and the practicability of the device is improved;
2. according to the invention, the guide block and the guide groove are arranged, so that the guide block can support the cylinder in the guide groove, and the guide groove can provide a long-distance transverse sliding surface for the guide block, so that the guide block can stably slide forwards and backwards, the problem of shaking deviation is avoided, and the position of the movable plate can be changed, so that the test tube jacking work can be achieved batch by batch, powerful help can be further provided for the observation work of a plurality of test tubes, and the use requirements of the observation test tubes are met;
3. according to the invention, the first spring is arranged, and under the action of the elastic thrust of the first spring, the sliding block can drive the frame to automatically reset through the sliding block, so that the work of accommodating the test tube into the groove can be controlled, the test tube can automatically complete coherent operation along with the downward movement of the movable plate, and meanwhile, the reagent can be effectively protected through the accommodation of the test tube.
Drawings
FIG. 1 is a schematic sectional view of the front view of the present invention;
FIG. 2 is a schematic top view of the present invention;
FIG. 3 is a schematic sectional view of a movable assembly of the present invention;
FIG. 4 is a schematic structural view of a top view of a partial cross section of the cylinder according to the present invention;
FIG. 5 is a schematic top view of the baffle of the present invention;
in the figure: 1 place the board, 2 recesses, 3 spouts, 4 sliders, 5 first springs, 6 reagents, 7 test tubes, 8 stopcocks, 9 frames, 10 through-holes, 11 second springs, 12 first pistons, 13 bearings, 14 baffles, 15 barrels, 16 place the hole, 17 handles, 18 connecting rods, 19 second pistons, 20 movable components, 201 movable plates, 202 movable sleeves, 203 bracing pieces, 204 fixed slots, 205 fixed blocks, 206 first pin shafts, 207 supporting blocks, 208 second pin shafts, 209 third pin shafts, 2010 connecting blocks, 2011 fourth pin shafts, 2012 movable rods, 21 guide grooves, 22 guide blocks, 23 connecting shafts.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As shown in figures 1-5, the invention provides a rapid DNA detection kit, which comprises a placing plate 1, wherein the upper surface of the placing plate 1 is provided with a plurality of grooves 2, the grooves 2 are arranged so as to accommodate test tubes 7 and a frame 9, the left side surface of the inner wall of each groove 2 and the right side surface of the inner wall of each groove 2 are respectively provided with a chute 3, a slide block 4 is connected in each chute 3 in a sliding manner, the slide block 4 can slide in each chute 3 more stably by arranging the slide block 4 and the chute 3, so that the frame 9 can slide up and down stably, opposite surfaces of the two slide blocks 4 are respectively fixedly connected with the left side surface and the right side surface of the frame 9, the frame 9 is arranged in a hollow shape, the blocking of sight line is avoided, so that reagents 6 in the test tubes 7 can be clearly viewed, the inner surface of the frame 9 is provided with a plurality of test tubes 7, the upper surface of test tube 7 is provided with stopcock 8, through setting up stopcock 8 to can play sealed effect to test tube 7, avoid impurity to get into, thereby can ensure the quality of 7 inside reagents of test tube 6, be provided with reagent 6 in the test tube 7, place the upper surface of board 1 and seted up through-hole 10, the left surface of the inner wall of through-hole 10 is linked together with the right flank of a plurality of 2 inner walls of recess.
A cylinder body 15 is arranged in the through hole 10, a first piston 12 is arranged on the inner surface of the cylinder body 15, a bearing 13 is clamped on the upper surface of the first piston 12, the connecting shaft 23 can rotate in the bearing 13 through the arrangement of the bearing 13, thereby being beneficial to the device to achieve the limiting effect by abutting the baffle plate 14 on the surface of the cylinder body 15 through rotation and avoiding the influence of the elastic reset of the first spring 5 on the operation of observing the reagent 6, the inner surface of the bearing 13 is sleeved with the connecting shaft 23, the inner surface of the cylinder body 15 is provided with the second piston 19, by providing the second piston 19 and the first piston 12, with the first piston 12 and the second piston 19 in a sealed state, therefore, the purpose of the air pressure pushing type operation of the device can be achieved, the left side face of the second piston 19 is fixedly connected with the right end of the connecting rod 18, the upper surface of the connecting rod 18 is fixedly connected with the lower surface of the movable assembly 20, and the lower surface of the movable assembly 20 is fixedly connected with the upper surface of the barrel 15.
As shown in FIG. 1, the upper surface of the inner wall of the chute 3 is fixedly connected with one end of a first spring 5, by arranging the first spring 5, and under the action of the elastic thrust of the first spring 5, the slider 4 can drive the frame 9 to automatically reset through the slider 4, so that the work of accommodating the test tube 7 into the groove 2 can be controlled, and the test tube can move down along with the movable plate 201 to automatically complete the coherent operation, meanwhile, the reagent 6 can be effectively protected by accommodating the test tube 7, the other end of the first spring 5 is fixedly connected with the upper surface of the slider 4, the lower surface of the first piston 12 is fixedly connected with one end of a second spring 11, by arranging the second spring 11, the elastic tension of the second spring 11 can drive the first piston 12 to downwards complete the reset, so that the reset operation among structures can be achieved, the use work is further more convenient, the other end of the second spring 11 is fixedly connected with the lower surface of the inner wall of the barrel 15, the left side surface of the inner wall of the through hole 10 and the right side surface of the inner wall are both provided with guide grooves 21, the guide blocks 22 can support the cylinder body 15 in the guide grooves 21 by arranging the guide blocks 22 and the guide grooves 21, and the guide grooves 21 can provide long-distance transverse sliding surfaces for the guide blocks 22, so that the guide blocks 22 can stably slide back and forth, the problem of shaking deviation is avoided, the device can further change the position of the movable plate 201, the test tubes 7 can be jacked up one by one, powerful help can be further provided for the observation work of a plurality of test tubes 7, the use requirements of the observation test tubes 7 are met, the guide blocks 22 are slidably connected to the inner surfaces of the guide grooves 21, the opposite surfaces of the two guide blocks 22 are fixedly connected with the left side surface and the right side surface of the cylinder body 15 respectively, and the outer surface of the connecting shaft 23 is fixedly connected with the inner surface of the baffle plate 14, the top of connecting axle 23 and the lower fixed surface of handle 17 are connected, through setting up handle 17, and handle 17 can provide the point of application of force, the work of controlling has been made things convenient for, and the rubber sleeve on handle 17 surface, not only can play anti-skidding effect, can also bring good operation travelling comfort, the hole 16 has been placed to the upper surface of barrel 15 has been seted up, place hole 16 through seting up, thereby can provide the activity space for connecting axle 23, guarantee the work of reciprocating of connecting axle 23, connecting axle 23 is located places the hole 16.
As shown in fig. 3, the movable assembly 20 includes two fixed blocks 205, the two fixed blocks 205 are respectively fixedly connected to the lower surface of the movable plate 201 and the upper surface of the cylinder 15, the front surfaces of the two fixed blocks 205 are respectively movably connected to the back surfaces of the two movable rods 2012 through the first pin 206, the opposite surfaces of the two movable rods 2012 are movably connected through the fourth pin 2011, the back surface of the front movable rod 2012 is movably connected to the front surface of the connecting block 2010 through the third pin 209, and the first pin 206, the second pin 208, the third pin 209 and the fourth pin 2011 are arranged to allow the first pin 206, the second pin 208, the third pin 209 and the fourth pin 2011 to provide a supporting point and a movable point for the movable rod 2012 to smoothly perform an angular swing operation, so as to ensure a normal control operation of the movable plate 201, the lower surface of connecting block 2010 is connected with the last fixed surface of connecting rod 18, the back of rear movable rod 2012 passes through the front swing joint of second round pin axle 208 and supporting shoe 207, the left side joint of supporting shoe 207 has movable sleeve 202, the internal surface of movable sleeve 202 has cup jointed bracing piece 203, through setting up movable sleeve 202 and movable rod 2012, it is more steady that feasible movable sleeve 202 can be slided about bracing piece 203 surface, thereby can ensure the work of the steady turned angle of fly leaf 201, the left end and the right-hand member of bracing piece 203 respectively with the left surface of fixed slot 204 inner wall and the right flank fixed connection of inner wall, the lower surface at fly leaf 201 is seted up to fixed slot 204.
A preparation method of a rapid DNA detection kit reagent comprises the following steps:
s1, a rapid DNA detection kit, which comprises liposome, betaine, ammonium sulfate, trehalose, calcium chloride, sodium azide, TritonX-1000 and deionized water, wherein the mass ratio of the liposome, the betaine, the ammonium sulfate, the trehalose, the calcium chloride, the sodium azide, the TritonX-1000 and the deionized water is 12:4:5:3:2:1: 73.
S2, adding raw materials of liposome and quantum dots into a reactor, adding chloroform, uniformly mixing at 35-45 ℃, evaporating chloroform, blow-drying with nitrogen or argon, adding phosphate buffer solution, ultrasonically oscillating for 4-6 min, extruding and filtering for multiple times by using a microporous filtering membrane to obtain a liposome mixture, adjusting the pH value of betaine, ammonium sulfate, trehalose, calcium chloride, sodium azide, TritonX-1000 and deionized water to 8.8 by using sodium hydroxide, and uniformly mixing the obtained liposome mixture, betaine, ammonium sulfate, trehalose, calcium chloride, sodium azide, TritonX-1000 and deionized water, liposome, betaine, ammonium sulfate, trehalose, calcium chloride, sodium azide, TritonX-1000 and deionized water to obtain a reagent 6.
The working principle of the invention is as follows:
s1, when the tube needs to be used, the handle 17 is pulled upwards, so that the handle 17 drives the connecting shaft 23 to move upwards, the connecting shaft 23 drives the first piston 12 to move upwards, meanwhile, the connecting shaft 23 drives the baffle 14 to move upwards, the second spring 11 extends, the second piston 19 drives the connecting rod 18 to move rightwards, the movable rod 2012 turns over to push the movable plate 201 to move upwards, the movable plate 201 pushes the frame 9 to move upwards, the frame 9 compresses the first spring 5 through the slider 4, meanwhile, the frame 9 can drive the test tube 7 to upwards separate from the groove 2, the connecting shaft 23 drives the baffle 14 to separate from the placing hole 16, the handle 17 is rotated again, the handle 17 drives the connecting shaft 23 to rotate, the connecting shaft 23 drives the baffle 14 to turn over, and the baffle 14 can abut against the surface of the barrel 15;
s2, observing the reagent 6, if the test tube 7 is completely observed, rotating the handle 17 to enable the handle 17 to drive the baffle 14 to rotate to ninety degrees through the connecting shaft 23, enabling the position of the baffle 14 to correspond to the position of the placing hole 16, loosening the handle 17, enabling the second spring 11 to drive the first piston 12 to move downwards through self-pulling force, enabling the second piston 19 to push the connecting rod 18 to move leftwards, enabling the movable rod 2012 to contract and driving the movable plate 201 to move downwards, and meanwhile, enabling the elastic force of the first spring 5 to push the frame 9 to move downwards through the sliding block 4 to enable the test tube 7 to be accommodated in the groove 2;
s3, finally, the position of the cylinder 15 can be moved by the handle 17, so that the movable plate 201 can be moved to the position, and when the movable plate 201 is moved to the bottom of the other frame 9, the above operation is continued to perform the observation of the reagent 6.
The points to be finally explained are: first, in the description of the present application, it should be noted that, unless otherwise specified and limited, the terms "mounted," "connected," "connecting," and "connecting" should be understood broadly, and may be a mechanical connection or an electrical connection, or a communication between two elements, and may be directly connected, and "upper," "lower," "left," and "right" are only used to indicate relative positional relationships, and when the absolute position of the object to be described is changed, the relative positional relationships may be changed;
secondly, the method comprises the following steps: in the drawings of the disclosed embodiments of the invention, only the structures related to the disclosed embodiments are referred to, other structures can refer to common designs, and the same embodiment and different embodiments of the invention can be combined with each other without conflict;
and finally: the above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that are within the spirit and principle of the present invention are intended to be included in the scope of the present invention.
Claims (2)
1. A quick DNA detect reagent box, including placing board (1), its characterized in that: the device comprises a placing plate (1) and is characterized in that a plurality of grooves (2) are formed in the upper surface of the placing plate (1), sliding grooves (3) are formed in the left side surface of the inner wall of each groove (2) and the right side surface of the inner wall of each groove (2), sliding blocks (4) are connected in the sliding grooves (3) in a sliding mode, opposite surfaces of the two sliding blocks (4) are fixedly connected with the left side surface and the right side surface of a frame (9) respectively, a plurality of test tubes (7) are arranged on the inner surface of the frame (9), tube plugs (8) are arranged on the upper surfaces of the test tubes (7), reagents (6) are arranged in the test tubes (7), through holes (10) are formed in the upper surface of the placing plate (1), and the left side surface of the inner wall of each through hole (10) is communicated with the right side surfaces of the inner walls of the grooves (2);
a cylinder (15) is arranged in the through hole (10), a first piston (12) is arranged on the inner surface of the cylinder (15), a bearing (13) is connected to the upper surface of the first piston (12) in a clamping mode, a connecting shaft (23) is sleeved on the inner surface of the bearing (13), a second piston (19) is arranged on the inner surface of the cylinder (15), the left side surface of the second piston (19) is fixedly connected with the right end of a connecting rod (18), the upper surface of the connecting rod (18) is fixedly connected with the lower surface of a movable assembly (20), and the lower surface of the movable assembly (20) is fixedly connected with the upper surface of the cylinder (15);
guide grooves (21) are formed in the left side surface of the inner wall of the through hole (10) and the right side surface of the inner wall, guide blocks (22) are connected to the inner surface of each guide groove (21) in a sliding mode, and the opposite surfaces of the two guide blocks (22) are fixedly connected with the left side surface and the right side surface of the cylinder body (15) respectively;
the outer surface of the connecting shaft (23) is fixedly connected with the inner surface of the baffle (14), the top end of the connecting shaft (23) is fixedly connected with the lower surface of the handle (17), a placing hole (16) is formed in the upper surface of the barrel (15), and the connecting shaft (23) is located in the placing hole (16);
the upper surface of the inner wall of the sliding chute (3) is fixedly connected with one end of a first spring (5), the other end of the first spring (5) is fixedly connected with the upper surface of the sliding block (4), the lower surface of the first piston (12) is fixedly connected with one end of a second spring (11), and the other end of the second spring (11) is fixedly connected with the lower surface of the inner wall of the cylinder (15);
the movable assembly (20) comprises two fixed blocks (205), the back surfaces of the two fixed blocks (205) are fixedly connected with the lower surface of the movable plate (201) and the upper surface of the cylinder (15) respectively, the front surfaces of the two fixed blocks (205) are movably connected with the back surfaces of the two movable rods (2012) respectively through first pin shafts (206), the opposite surfaces of the two movable rods (2012) are movably connected through fourth pin shafts (2011), the back surface of the front movable rod (2012) is movably connected with the front surface of a connecting block (2010) through a third pin shaft (209), the lower surface of the connecting block (2010) is fixedly connected with the upper surface of the connecting rod (18), the back surface of the rear movable rod (2012) is movably connected with the front surface of a supporting block (207) through a second pin shaft (208), and a movable sleeve (202) is clamped on the left side surface of the supporting block (207), the inner surface of the movable sleeve (202) is sleeved with a supporting rod (203), the left end and the right end of the supporting rod (203) are fixedly connected with the left side surface of the inner wall of the fixed groove (204) and the right side surface of the inner wall respectively, and the fixed groove (204) is formed in the lower surface of the movable plate (201);
the guide block (22) supports the barrel (15) in the guide groove (21), the guide groove (21) provides a long-distance transverse sliding surface for the guide block (22), the guide block (22) slides stably in the front and at the back, the problem of swinging and deflection is avoided, the position of the movable plate (201) can be changed, the test tubes (7) can be jacked up batch by batch, the position of the barrel (15) is moved through the handle (17), the movable plate (201) moves in position, and when the movable plate (201) moves to the bottom of the other frame (9), the operation is continuously carried out to observe the reagent (6);
when the test tube fixing device is used, the handle (17) is pulled upwards, the handle (17) drives the connecting shaft (23) to move upwards, the connecting shaft (23) drives the first piston (12) to move upwards, meanwhile, the connecting shaft (23) drives the baffle (14) to move upwards, the second spring (11) extends, the second piston (19) drives the connecting rod (18) to move rightwards, the movable rod (2012) is turned over, the movable plate (201) pushes the frame (9) to move upwards, the frame (9) compresses the first spring (5) through the sliding block (4), and meanwhile, the frame (9) drives the test tube (7) to upwards separate from the groove (2).
2. A method for preparing a reagent stored in the rapid DNA detection kit according to claim 1, comprising the steps of:
s1, the reagent comprises liposome, betaine, ammonium sulfate, trehalose, calcium chloride, sodium azide, TritonX-1000 and deionized water, and the mass ratio of the liposome, the betaine, the ammonium sulfate, the trehalose, the calcium chloride, the sodium azide, the TritonX-1000 and the deionized water is 12:4:5:3:2:1: 73;
s2, adding raw materials of liposome and quantum dots into a reactor, adding chloroform, uniformly mixing at 35-45 ℃, evaporating chloroform, blow-drying with nitrogen or argon, adding phosphate buffer solution, ultrasonically oscillating for 4-6 min, extruding and filtering for multiple times by using a microporous filtering membrane to obtain a liposome mixture, adjusting the pH value of betaine, ammonium sulfate, trehalose, calcium chloride, sodium azide, TritonX-1000 and deionized water to 8.8 by using sodium hydroxide, and uniformly mixing the obtained liposome mixture, betaine, ammonium sulfate, trehalose, calcium chloride, sodium azide, TritonX-1000 and deionized water, liposome, betaine, ammonium sulfate, trehalose, calcium chloride, sodium azide, TritonX-1000 and deionized water to obtain a reagent (6).
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| CN114919870A (en) * | 2021-11-02 | 2022-08-19 | 江苏艾洛特医药研究院有限公司 | Protein purification kit |
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