CN112877259B - Staphylococcus capable of efficiently degrading polypropylene, degradation microbial inoculum and application thereof - Google Patents

Abstract

The invention discloses a Staphylococcus for efficiently degrading polypropylene, a degrading microbial inoculum and application thereof, wherein the strain is identified as Staphylococcus (Staphylococcus sp.), and is preserved in China center for type culture collection (CCTCC NO): m2021014. The polypropylene degrading strain is obtained by separation and screening, can be effectively used for biologically degrading polypropylene, such as polypropylene plastic films, particles and powder, the maximum degradation rate of the polypropylene within 60 days is 5.0 percent by adopting the strain to degrade the polypropylene plastic, a feasible technical means is provided for utilizing the biologically degraded polypropylene plastic waste, the strain or a microbial inoculum thereof is applied to a refuse dump, the polypropylene plastic waste in the refuse can be effectively degraded, the landfill capacity of the refuse landfill is improved, and remarkable economic benefit and environmental protection benefit can be created.

Description

Staphylococcus capable of efficiently degrading polypropylene, degradation microbial inoculum and application thereof

Technical Field

The invention relates to microbial remediation of environmental pollution, in particular to a staphylococcus for efficiently degrading polypropylene, a degrading microbial inoculum thereof and application thereof.

Background

Plastics are an indispensable important component in our lives, and are used for various applications such as packaging, agriculture, textiles, disposable goods and the like. The most commonly used plastics are polyolefins such as polyethylene and polypropylene, which are biologically inert in nature due to their high molecular weight, hydrophobicity and lack of functional groups. This leads to the accumulation of these substances in the environment, the disposal of which is a major concern worldwide. Despite some control measures, the amount of trash in the ocean is increasing. These wastes also pose a threat to terrestrial wildlife by entangling or blocking their digestive pathways. Approximately 1.4 million tons of synthetic polymers are produced annually throughout the world. Polypropylene is the second largest polymer to polyethylene only. It is a thermoplastic polymer produced from propylene units using ziegler-natta catalysts, is lightweight, resistant to chemicals, acids, solvents, and has flexibility. The global polypropylene demand is 5, 845 ten thousand tons in 2014, and is expected to reach 8, 735 ten thousand tons by 2022, and the composite annual growth rate is 5.2% from 2015 to 2022. As the use of polymers becomes unavoidable, there must be a way to degrade these polymers.

At present, incineration and landfill are the most common disposal methods. In the burning process, a large amount of harmful gases such as carbon dioxide, nitrogen oxides, dioxin and the like can be generated, and secondary pollution is brought to the environment; in order to solve the problem of environmental pollution caused by waste plastics, researchers in various countries around the world are striving to find a method for effectively treating plastics, wherein biodegradation is a method with great potential.

Disclosure of Invention

The purpose of the invention is as follows: aiming at the problems in the prior art, the invention provides a strain LICME-ZWZR-13 capable of efficiently degrading polypropylene, which is identified as Staphylococcus (Staphylococcus sp.). The strain LICME-ZWZR-13 can be effectively used for biodegradation of polypropylene, especially polypropylene plastics, such as waste polypropylene plastic films, polypropylene plastic particles, polypropylene plastic powder and the like.

The invention also aims to provide a separation and screening method of the polypropylene high-efficiency degradation strain LICME-ZWZR-13 and a microbial inoculum produced by the method; the degrading microbial inoculum prepared by the strain can degrade the waste polypropylene plastics remained in the environment in a short time. The degradation microbial inoculum can be produced by fermentation equipment commonly used in the fermentation industry, and has the advantages of low production cost, convenient use and good degradation effect.

The invention also provides a method for degrading polypropylene waste, which comprises the following steps: the strain and the microbial inoculum are directly contacted with the polypropylene plastic, so that the polypropylene waste can be produced by a biodegradation method.

The technical scheme is as follows: in order to achieve the aim, the high-efficiency degradation polypropylene strain LICME-ZWZR-13 is identified as Staphylococcus (Staphylococcus sp.), is preserved in China center for type culture collection (CCTCC NO): m2021014.

The high-efficiency degradation polypropylene strain LICME-ZWZR-13 comprises the following steps:

a sample to be separated is subjected to directional enrichment by being connected to a culture medium which takes polypropylene as a unique carbon source, and nutrient agar is subjected to streak separation, so that a degradation strain which can utilize the polypropylene as the carbon source and energy is obtained.

Further, the method for screening degradable plastic strains comprises the following steps: activating sediment samples containing various mixed bacteria obtained from a refuse landfill in 0.9% of physiological saline to obtain bacterial suspension; culturing at least a portion of the obtained bacterial suspension using an inorganic salt medium and polypropylene plastic as the sole carbon source, so as to obtain a culture broth containing said plastic-degrading microorganisms. And culturing the culture solution containing the degradable plastic microorganisms by using a solid LB culture medium so as to obtain single colonies of the degradable plastic microorganisms.

Wherein the culture medium with polypropylene as the only carbon source comprises the following components: k ₂ HPO ₄ 0.5g，KH ₂ PO ₄ 0.04g，NaCl 0.1g，CaCl ₂ ·2H ₂ O 0.002g，(NH ₄ ) ₂ SO ₄ 0.2g，MgSO ₄ ·7H ₂ O 0.02g，FeSO ₄ 0.001g; the reagent is dissolved in 1L of deionized water, and the pH is adjusted to 7.8, thus obtaining the liquid culture medium.

Wherein the sample to be separated is a refuse landfill sediment.

The degradation microbial inoculum for efficiently degrading the polypropylene has the active component of staphylococcus.

The preparation method of the degradation microbial inoculum for efficiently degrading polypropylene comprises the following steps:

(1) Taking a test tube liquid obtained by culturing the strain LICME-ZWZR-13 to the logarithmic phase as a first-level seed liquid, inoculating the first-level seed liquid into a fermentation culture medium according to the volume ratio of the inoculation amount of not less than 0.5%, and performing shake culture to the logarithmic phase to obtain a fermentation strain;

(2) Inoculating the prepared fermentation strain into a culture medium of a seeding tank according to the volume ratio of 10-20%, and culturing to logarithmic phase to prepare a secondary seed solution;

(3) Inoculating the secondary seed liquid into a culture medium in a production tank for culture and fermentation, wherein the inoculation amount is not less than 0.2% in volume ratio, the ventilation amount of sterile air in the culture process is not less than 1.1, the stirring speed is not less than 50rpm, the culture temperature is 20-30 ℃, the culture time is not less than 12h, and the culture liquid after fermentation is taken out of the tank and directly subpackaged into a liquid agent, or is prepared into powder through drying, or is prepared into a high-purity preparation through steps of separation, purification and the like.

Wherein the fermentation medium comprises 1% of glucose and K ₂ HPO ₄ 0.2％，KH ₂ PO ₄ 0.2％，NH ₄ NO ₃ 0.1％，MgSO ₄ .7H ₂ O 0.05％，NaCl 0.01％，FeSO ₄ .7H ₂ O 0.03％，ZnSO ₄ .7H ₂ O 0.03％，MnSO ₄ .7H ₂ 0.03 percent of O, 0.03 percent of yeast extract and 7.8 percent of pH.

The strain LICME-ZWZR-13 is applied to the biodegradation of polypropylene.

The degrading bacteria agent is applied to the biodegradation of polypropylene.

The polypropylene is polypropylene plastic, and comprises at least one of polypropylene plastics with various specifications and shapes, and specifically comprises the following components: polypropylene plastic film, polypropylene plastic particles or polypropylene plastic powder.

Wherein the strain or the microbial inoculum is contacted with the polypropylene plastic, and the waste of the polypropylene plastic is treated by a biodegradation method.

Further, the polypropylene plastic is polypropylene plastic waste existing in a landfill or various water bodies.

Has the advantages that: compared with the prior art, the invention has the following advantages:

the invention obtains the polypropylene high-efficiency degradation strain staphylococcus LICME-ZWZR-13 through separation and screening, which can be used for biologically degrading polypropylene, in particular to waste powdery, flaky and granular polypropylene plastics in a degradation environment; the strain LICME-ZWZR-13 can grow by taking polypropylene as a unique carbon-nitrogen source, has good polypropylene degradation characteristic, provides new resources and ideas for bioremediation of polypropylene waste in the environment, and has wide application prospect.

The strain has good degradation effect on plastic polypropylene waste, and the maximum degradation rate of the strain for degrading polypropylene plastic in 60 days is 5.0%, so that a feasible technical means is provided for degrading the polypropylene plastic waste by using the organisms. The strain or the microbial inoculum thereof is applied to a refuse dump, so that the polypropylene plastic waste in the refuse can be effectively degraded, the landfill capacity of the refuse dump is improved, and remarkable economic benefit and environmental protection benefit can be created. The bacterial strain is used for degradation, is green and pollution-free, and has important significance for protecting environment, protecting human health and reducing the treatment cost of garbage.

Drawings

FIG. 1 shows the appearance of polypropylene in a scanning electron microscope after the polypropylene degrading bacteria provided by the invention degrade for 60 days;

FIG. 2 is a graph showing the growth of the polypropylene degrading bacteria provided by the present invention;

FIG. 3 is a colony morphology characteristic diagram of the polypropylene degrading bacteria provided by the present invention;

FIG. 4 is a comparison of Fourier near infrared spectra, i.e., changes in functional groups, before and after degradation of polypropylene provided by the present invention;

FIG. 5 shows the quality change of the polypropylene provided by the present invention after 60 days of degradation.

Detailed Description

The invention is further illustrated by the following figures and examples.

The starting materials and reagents used in the present invention are commercially available unless otherwise specified.

Example 1

Screening and isolation of staphylococci

Separating and screening the sediment of the Nanjing large-scale refuse landfill as a sample with separation:

weighing 1g of collected sediment sample, placing in a sterile conical flask, adding 9ml of 0.9% by mass physiological saline, shake culturing at 30 deg.C and 180rpm in a constant temperature shaking incubator for 5h to obtain bacterial suspension, and diluting with 0.9% physiological saline to 10% by volume ^-1 、10 ^-2 、10 ^-3 、10 ^-4 、10 ^-5 、10 ^-6 . Transferring 1ml of the gradient diluted bacterial suspension into a culture medium of mineral salts without carbon sources, adding 2g of polypropylene particles (polypropylene powder, mn 4000 +/-500) which are soaked in alcohol and irradiated with ultraviolet, culturing at 30 ℃ and 180rpm for 5h, and arranging three groups in parallel. And after the culture system becomes turbid, picking the bacterial liquid on nutrient agar, and streaking and purifying the separated bacterial strain for multiple times. And (3) after the separated single colony strain is stored, verifying the utilization capacity of the polypropylene on a carbon-source-free mineral salt liquid culture medium. 2g/L of polypropylene particles soaked in alcohol and irradiated by ultraviolet are added into a carbon-source-free mineral salt liquid culture medium, and strains which grow rapidly are selected for culture and preservation.

The culture medium for separation and screening is a carbon source-free mineral salt culture medium, and comprises the following components: k ₂ HPO ₄ 0.5g，KH ₂ PO4 0.04g，NaCl 0.1g，CaCl ₂ .2H ₂ O 0.002g，(NH ₄ ) ₂ SO ₄ 0.2g，MgSO ₄ .7H ₂ O 0.02g，FeSO ₄ 0.001g. Dissolving the above reagents in 1L deionized water, adjusting pH to 7.8, adding polypropylene as the only carbon source, and sterilizing. Through the separation and screening work and multiple separation and purification, a strain capable of growing by using polypropylene as a carbon source and an energy source is obtained. It was named as LICME-ZWZR-13. The cells were cultured in LB medium at 30 ℃ and 180rpm, and gram-stained until logarithmic phase. Under an optical microscope, the strain is purple and positive through gram staining. The growth curve in LB medium is shown in FIG. 2, where growth is rapid, entering log phase after 4 hours, and after 6 hoursAnd entering a stable period. As shown in FIG. 3, the cultured colonies were round, smooth-edged, glossy, and pale yellow.

Example 2

Molecular biological characterization of strains

The DNA of the single strain obtained by separation is extracted by a kit, and PCR amplification is carried out by taking bacterial universal primers 27F and 1492R as amplification primers.

16S rDNA sequence primer 27F (SEQ ID NO. 2): 5'-AGA GTT TGA TCC TGG CTC AG-3' of the formula,

1492R(SEQ ID NO.3)：5’-GGT TAC CTT GTT ACG ACT T-3’。

the amplified products were subjected to electrophoresis detection, and sequencing was performed by Okinsoniaceae (Nanjing) Biochemical company. The resulting sequences were subjected to BLAST analysis with sequences already available in the NCBI database.

Alignment of the 16S rDNA sequence (SEQ ID NO. 1) of the strain LICME-ZWZR-13 at NCBI revealed that similar to Staphyloccus sciuri strain NXUBIHAL013, combined with the physiological and biochemical characteristics of the strain, was initially identified as Staphylococcus (Staphyloccus sp.) and named Staphylococcus LICME-ZR-13 (Staphyloccus sp. The strain LICME-ZWZR-13 is delivered to the China Wuhan, china center for type culture Collection (CCTCC for short) for preservation, the preservation time is 2021 year, 1 month and 5 days, and the preservation number is CCTCC NO: m2021014.

SEQ ID NO.1：

CTCCACCGGCTTCGGGTGTTACAAACTCTCGTGGTGTGACGGGCGGTGTGTACAAGACCCGGGAACGTATTCACCGTAGCATGCTGATCTACGATTACTAGCGATTCCAGCTTCATGTAGTCGAGTTGCAGACTACAATCCGAACTGAGAATAATTTTATGGGATTTGCTTGGCCTCGCGGATTCGCTGCCCTTTGTATTATCCATTGTAGCACGTGTGTAGCCCAAATCATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCAACCTAGAGTGCCCAACTTAATGATGGCAACTAAGCTTAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAACCATGCACCACCTGTCACTTTGTCCCCCGAAGGGGAAGACTCTATCTCTAGAGCGGTCAAAGGATGTCAAGATTTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATGCTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTGAGTTTCAACCTTGCGGTCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTGCAGCACTAAGGGGCGGAAACCCCCTAACACTTAGCACTCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAGAGCCGCCTTCGCCACTGGTGTTCCTCCATATCTCTGCGCATTTCACCGCTACACATGGAATTCCACTCTCCTCTTCTGCACTCAAGTTTCCCAGTTTCCAATGACCCTCCACGGTTGAGCCGTGGGCTTTCACATCAGACTTAAGAAACCGCCTACGCGCGCTTTACGCCCAATAATTCCGGATAACGCTTGCCACCTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTGGTTAGGTACCGTCAAGACTTGTTCAGTTACTAACAAATTTGTTCTTCCCTAACAACAGAGTTTTACGATCCGAAGACCTTCATCACTCACGCGGCGTTGCTCCGTCAGGCTTTCGCCCATTGCGGAAGATTCCCTACTGCTGCCTCCCGTAGGAGTCTGGACCGTGTCTCAGTTCCAGTGTGGCCGATCACCCTCTCAGGTCGGCTACGTATCGTCGCCTTGGTAAGCCGTTACCTTACCAACTAGCTAATACGGCGCGGGTCCATCTATAAGTGACAGCCGAAACCGTCTTTCACTATTGAACCATGCGGTTCAAAATATTATCCGGTATTAGCCCCGGTTTCCCGGAGTTATCCCAGTCTTATAGGTAGGTTACCCACGTGTTACTCACCCGTCCGCCGCTAACATCAGAGAAGCAAGCTTCTCATCTTTCGCTCGACTGCA

Example 3

Degradation of polypropylene film plastic in solid inorganic salt culture medium by staphylococcus LICME-ZWZR-13

1. Staphylococcus strain LICME-ZWZR-13 obtained in example 1 was inoculated into a 50ml Erlenmeyer flask containing a broth medium and cultured by fermentation at 30 ℃ and 180rpm for 5 hours to obtain a culture solution. Centrifuging, resuspending with physiological saline for 3 times, washing off original culture medium, ensuring that no carbon source and other nutrients exist in the bacterial suspension, measuring the OD600 value of the bacterial suspension, and diluting by 10 times.

2. Taking 5ml of the diluted bacterial suspension by using a pipette, and dripping the bacterial suspension into the center of a solid inorganic salt culture medium for coating culture. After the coating, a prepared 5cm x 5cm polypropylene film which is sterilized by 75% alcohol and irradiated by ultraviolet is laid on the surface of the culture medium, the polypropylene film is fully contacted with the culture medium and the bacterial liquid, and the culture medium is placed in a constant temperature box at the temperature of 30 ℃ for culturing for 60 days.

3. The degradation of polypropylene was confirmed by SEM, FTIR by first washing with 0.01M phosphate buffered saline (PBS, pH 7.2) for two minutes, then with 2% SDS solution, with warm distilled water, then in 2.5% glutaraldehyde solution for 2h, then ultrasonically washing twice with 50% ethanol (once for 30 min), then overnight in 70% ethanol, finally washing three times with 100% ethanol (once for 30 min), and finally drying in an oven at 60 ℃.

The SEM of the graph a in FIG. 1 shows that the surface of the film is smooth when the film is not degraded, while the surface of the polypropylene film of the graph b after the degradation of the strain has obvious holes and becomes rough.

And (3) measuring the functional groups on the surface of the polypropylene plastic film by using a Fourier infrared spectrometer, and naturally drying the cleaned diaphragm for measuring. FTIR scanning wavelength range 500-4000cm in FIG. 4 ^-1 Resolution 4cm ^-1 The obvious structural change of the polypropylene surface is obviously observed after 32 times of scanning, and the structural change is at 600cm ^-1 The new peak appears at the position of the compound (A), which is a basic sign of the biodegradation of the polypropylene plastic and indicates that the polypropylene is biodegraded.

Example 4

Degradation of polypropylene particle plastic in liquid inorganic salt culture medium by staphylococcus

To a 250ml Erlenmeyer flask, 10ml of the suspension (the final diluted suspension in step 1 of example 3) and 90ml of a liquid inorganic salt medium were added, 2g of polypropylene plastic particles were added to the Erlenmeyer flask, and the mixture was subjected to spinner culture in a shaker at 30 ℃ at 180 rpm. 3 replicates were set on days 7, 14, 21, 28, and 60, respectively, while a control group was set without adding the bacterial suspension. The mass of polypropylene was calculated at the corresponding points. The mass attenuation ratio (%) of the polypropylene plastic particles (%) = (initial mass of polypropylene-mass after degradation)/initial mass 100%. As shown in FIG. 5, after 60 days, the weight loss was 5.0%, while the control treatment was unchanged, indicating that polypropylene was degraded by the strain LICME-ZWZR-13.

Degradation of polypropylene was confirmed by SEM and FTIR (same as in example 3)

Degradation effect: in FIG. 1c, the untreated polypropylene particles are smooth in surface, while in d, the polypropylene particles degraded by the bacterial strain become rough in surface and have pores which are significantly eroded by the microorganisms.

In fig. 4, the measurement of the functional groups on the plastic surface of the polypropylene particles is performed by using a fourier infrared spectrometer, and the measurement is performed after the polypropylene particles cleaned are naturally dried. Wherein the FTIR scanning wavelength range is 1000-3500cm ^-1 Resolution 4cm ^-1 The obvious structural change of the polypropylene surface is obviously observed after 32 times of scanning, and is at 1640cm ^-1 The new peak C = C double bond appears at the position of the (A), which is a basic sign of the biodegradation of polypropylene plastics and indicates that the polypropylene is biodegraded. Thus, the strains of the invention can also degrade polypropylene particles.

Example 5

Preparation of degrading microbial inoculum of strain LICME-ZWZR-13

(1) Taking a test tube liquid obtained by culturing the strain LICME-ZWZR-13 to the logarithmic phase as a first-level seed liquid, inoculating the first-level seed liquid to a fermentation culture medium according to the volume ratio of 1% of the inoculation amount, and performing shaking culture to the logarithmic phase to obtain a fermentation strain;

(2) Inoculating the prepared fermentation strain into a culture medium of a seeding tank according to the volume ratio of 10%, and culturing to logarithmic phase to prepare a secondary seed solution;

(3) Inoculating the secondary seed liquid into a culture medium in a production tank for culture and fermentation, wherein the inoculation amount is 0.5% in volume ratio, the ventilation amount of sterile air in the culture process is 1.

The fermentation medium comprises 1% of glucose and K ₂ HPO ₄ 0.2％，KH ₂ PO ₄ 0.2％，NH ₄ NO ₃ 0.1％，MgSO ₄ .7H ₂ O 0.05％，NaCl 0.01％，FeSO ₄ .7H ₂ O 0.03％，ZnSO ₄ .7H ₂ O 0.03％，MnSO ₄ .7H ₂ 0.03 percent of O, 0.03 percent of yeast extract and 7.8 percent of pH.

Example 6

Preparation of degradation microbial inoculum of strain LICME-ZWZR-13

(1) Taking a test tube liquid obtained by culturing the strain LICME-ZWZR-13 to the logarithmic phase as a first-level seed liquid, inoculating the first-level seed liquid into a fermentation culture medium according to the volume ratio of 0.5 percent of the inoculation amount, and performing shaking culture to the logarithmic phase to obtain a fermentation strain;

(2) Inoculating the prepared fermentation strain into a culture medium in a seeding tank according to the volume ratio of 20%, and culturing to logarithmic phase to prepare a secondary seed solution;

(3) Inoculating the secondary seed liquid into a culture medium in a production tank for culture and fermentation, wherein the inoculation amount is 0.2% by volume, the ventilation amount of sterile air in the culture process is 1.

The fermentation medium comprises 1% of glucose and K ₂ HPO ₄ 0.2％，KH ₂ PO ₄ 0.2％，NH ₄ NO ₃ 0.1％，MgSO ₄ .7H ₂ O 0.05％，NaCl 0.01％，FeSO ₄ .7H ₂ O 0.03％，ZnSO ₄ .7H ₂ O 0.03％，MnSO ₄ .7H ₂ 0.03 percent of O, 0.03 percent of yeast extract and 7.8 percent of pH.

Sequence listing

<110> university of Nanjing university

<120> staphylococcus for efficiently degrading polypropylene as well as degradation microbial inoculum and application thereof

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Claims (8)

1. A strain LICME-ZWZR-13 for efficiently degrading polypropylene, identified as Staphylococcus (Staphylococcussp.), preserved in China center for type culture Collection with the preservation time of 2021 year, 1 month and 5 days and the preservation number of CCTCC NO: m2021014.

2. A degradation microbial inoculum for efficiently degrading polypropylene, which is characterized in that the active component of the microbial inoculum is staphylococcus LICME-ZWZR-13 as claimed in claim 1.

3. The preparation method of the degradation microbial inoculum for efficiently degrading the polypropylene according to claim 2, which is characterized by comprising the following steps:

(1) Taking a test tube liquid obtained by culturing the strain LICME-ZWZR-13 to the logarithmic phase as a first-level seed liquid, inoculating the first-level seed liquid into a fermentation culture medium according to the volume ratio of the inoculation amount of not less than 0.5%, and performing shake culture to the logarithmic phase to obtain a fermentation strain;

(2) Inoculating the prepared fermentation strain into a culture medium of a seeding tank according to the volume ratio of 10-20%, and culturing to logarithmic phase to prepare a secondary seed solution;

(3) Inoculating the secondary seed liquid into a culture medium in a production tank for culture and fermentation, wherein the inoculation amount is not less than 0.2% by volume, the ventilation volume of sterile air in the culture process is not less than 1.1, the stirring speed is not less than 50rpm, the culture temperature is 20-30 ℃, the culture time is not less than 12h, and the culture liquid after fermentation is directly taken out of the tank and is directly packaged into a liquid agent, or is prepared into powder through drying or is prepared into a high-purity preparation through a separation and purification step.

4. Use of the strain LICME-ZWZR-13 according to claim 1 for the biodegradation of polypropylene.

5. The use of the degrading microbial agent of claim 2 in the biodegradation of polypropylene.

6. Use according to claim 4 or 5, wherein the polypropylene is a polypropylene plastic, including at least one of polypropylene plastics of various sizes and shapes.

7. The use of claim 6, wherein the strain or the microbial inoculum is contacted with the polypropylene plastic, and the polypropylene plastic waste is treated by a biodegradation method.

8. Use according to claim 6, characterized in that the polypropylene plastic is polypropylene plastic waste present in landfills or in various bodies of water.

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