CN112875874A - In-situ reinforced repair method for underground water polluted by chlorohydrocarbon - Google Patents
In-situ reinforced repair method for underground water polluted by chlorohydrocarbon Download PDFInfo
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- 239000004135 Bone phosphate Substances 0.000 description 1
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Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/28—Treatment of water, waste water, or sewage by sorption
- C02F1/283—Treatment of water, waste water, or sewage by sorption using coal, charred products, or inorganic mixtures containing them
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/36—Organic compounds containing halogen
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/06—Contaminated groundwater or leachate
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- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Water Treatment By Sorption (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
The invention discloses an in-situ reinforced repair method for underground water polluted by chlorohydrocarbon. The method comprises the following steps: (1) investigating hydrogeology and pollutant distribution conditions of a polluted site; (2) calculating the size of underground water pollution plume, and defining a pollution area according to the size of the pollution plume; (3) preparing nano colloid activated carbon-based composite bacteria, and mixing the nano colloid activated carbon-based composite bacteria with water according to a ratio of 1: 10-1: 1000 to prepare a mixed microbial inoculum; (4) establishing injection points in each pollution area, pushing the mixed microbial inoculum to a target pollution layer through a direct-pushing drilling machine injection system, and sequentially injecting the mixed microbial inoculum into each injection point under certain pressure. The method of the invention has simple operation, can greatly reduce the injection depth and the injection cost, and has the advantages of fast removing speed of the chlorohydrocarbon in the underground water, thorough degradation, shortened repair period and reproducible adsorption and degradation capability.
Description
Technical Field
The invention relates to the technical field of groundwater pollution remediation, in particular to a colloidal active carbon composite bacteria in-situ reinforced groundwater remediation method capable of remedying chlorohydrocarbon pollution of different concentrations.
Background
Chlorinated Hydrocarbons (CHCs) are a class of volatile organic compounds with very strong "tri-basic" poisoning effects and bio-enrichment capabilities, represented by trichloroethylene, trichloroethane, and dichloroethane. It has been widely used in electronics, leather, dry cleaning and chemical industries, but due to improper storage and disposal, it is very easy to enter into the surrounding environments such as water, air and soil through volatilization, leakage and the like in the production process, once it enters into the underground environment, the chlorinated hydrocarbon is very easy to migrate and diffuse, causing long-term and long-lasting pollution in large area. Therefore, the research on the repair technology of the chlorinated hydrocarbon polluted underground water is of great practical significance.
The method for removing the chlorinated hydrocarbons in the underground water by utilizing microbial reduction and dechlorination is an environment-friendly and low-cost repairing technology. In the process, chlorohydrocarbon is used as an electron acceptor, and chlorine atoms are gradually replaced by hydrogen atoms through the action of microorganisms to achieve the effect of degradation and removal. Dechlorination (respiration) and Reduction of Iron Dissimilatory (Reduction of Iron Dissimilatory) are two important modes of action for reductive dechlorination of microorganisms. Dechlorination is a process in which microorganisms directly reduce and dechlorinate by using hydrogen, formic acid, acetic acid, pyruvic acid, etc. as electron donors and chlorine-containing organic substances as terminal electron acceptors through electron transfer in the respiratory chain on the cell membrane. The dissimilatory iron reduction refers to a process that dissimilatory iron reducing bacteria transfer electrons generated after the oxidation of an organic carbon source to the surface of iron oxide serving as an electron acceptor outside cells through respiration to reduce Fe (II) into Fe (III), and the essence of the dissimilatory iron reduction is electron transfer on an extracellular respiratory chain. However, the ecological toxicity of chlorinated hydrocarbons and the complex environmental conditions of underground water inhibit the reductive dechlorination capability of the above functional microorganisms, so that the natural biodegradation efficiency of chlorinated hydrocarbons in underground water is low.
In view of the above problems, in-situ enhanced bioreduction dechlorination technology has attracted attention, which provides suitable nutrients, electron donors and the like to the underground environment to achieve the purpose of promoting biodegradation of chlorinated hydrocarbons. The repairing material is one of the important factors influencing the in-situ reinforced biological reduction dechlorination effect. At present, the commonly used repair materials comprise zero-valent iron and an organic carbon source, but the repair materials have the problems of easy agglomeration and oxidation, short action time, low utilization efficiency and the like. And pollutants are easily distributed in aquifers of different geologies, and the general biological active matrix and zero-valent iron have small liquidity in underground water, so that certain difficulty exists in injection. Moreover, in a general polluted site, the pollution distribution of the halogenated hydrocarbon is not uniform and usually shows regional characteristics, and in low-concentration polluted soil, the biodegradation efficiency is very low, so that an obvious repairing effect is difficult to achieve.
Disclosure of Invention
The invention provides a method for in-situ reinforced repair of underground water polluted by chlorohydrocarbon by using colloidal activated carbon composite bacteria, aiming at solving the problems in the prior art. The colloidal activated carbon-based composite bacteria freely diffuse to the restoration radius range, and the purpose of restoring the underground water is realized through the physical-chemical-biological comprehensive action.
The purpose of the invention is realized by the following technical scheme:
an in-situ reinforced repair method for underground water polluted by chlorohydrocarbon, which comprises the following steps:
step 1, investigating hydrogeology and pollutant distribution conditions of a polluted site;
step 2, calculating the size of the pollution plume of the underground water, and defining a pollution area according to the size of the pollution plume;
step 3, preparing nano colloid activated carbon-based composite bacteria, and mixing the nano colloid activated carbon-based composite bacteria with water according to the proportion of 1: 10-1: 1000 to prepare a mixed microbial inoculum;
and 4, establishing injection points in each pollution area, pushing the mixed microbial inoculum to a target pollution layer through a direct-pushing drilling machine injection system, and sequentially injecting the mixed microbial inoculum into each injection point under certain pressure.
Further, a long-term monitoring well is established at the downstream of the polluted area, sampling monitoring after repair is carried out regularly, and the repair effect is evaluated.
Further, in the step 3, the specific steps of preparing the nano-colloid activated carbon-based composite bacteria are as follows:
step 31, grinding the solid activated carbon to ensure that the particle size range is 0.1-1.8 mu m; the activated carbon is one of shaddock peel activated carbon, coconut shell activated carbon or corn straw, and the specific surface area of the activated carbon is more than 1050 square meters per gram;
step 32, mixing the ground activated carbon into distilled water at 65 ℃, homogenizing for many times, and naturally cooling to room temperature to obtain stable nano colloidal activated carbon uniformly suspended in water; the particle size of the nano colloidal active carbon is 10-180 nm;
step 33, mixing the efficient chlorohydrocarbon degradation microbial inoculum and the nano colloidal activated carbon matrix prepared in the step 32 in a ratio of 1: 19 are mixed uniformly. The efficient chlorinated hydrocarbon degrading microbial inoculum is screened by the following processes: collecting an underground water sample polluted by chlorohydrocarbon; diluting and coating an underground water sample on a chlorohydrocarbon-containing plate culture medium to culture colonies; and (3) carrying out streak separation and purification, and repeatedly domesticating the purified strain on a fresh culture medium plate containing the chlorohydrocarbon for many times to obtain the efficient chlorohydrocarbon degrading microbial inoculum.
Further, the culture medium containing the chlorohydrocarbon comprises the following components in parts by weight: the ratio of the liquid inorganic salt culture medium to the chlorinated hydrocarbon polluted underground water sample is 125: 1.
Further, the composition of the liquid inorganic salt culture medium is as follows: NH (NH)4NO3:1650mg/L，KNO3:1900mg/L，CaCl2·2H2O:440mg/L，MgSO4·7H2O:370mg/L，KH2PO4:170mg/L，Na2-EDTA:37.3mg/L，FeSO4·7H2O:27.8mg/L，H3BO3:6.2mg/L，MnSO4·4H2O:22.3mg/L，ZnSO4·7H2O:8.6mg/L，KI:0.83mg/L，Na2MoO·2H2O:0.25mg/L，CuSO4·5H2O:0.025mg/L，CoCl2·6H2O:0.025mg/L。
Further, the direct-drive drilling machine injection system comprises a microbial inoculum configuration system, an injection pump system and a direct-drive injection system, wherein the microbial inoculum configuration system is used for carrying out configuration work of dissolving, diluting and stirring on the nano colloid activated carbon-based composite bacteria through a microbial inoculum stirrer; the injection pump system provides power through a hydraulic system of the drilling machine, boosts and propels the mixed microbial inoculum to a pressure pipeline, and then propels the injection drill rod and the drill bit to an injection point through a direct-pushing injection system of the drilling machine for injection.
The colloidal activated carbon complex microbial inoculum is an extremely fine suspension of charged particles, can prevent agglomeration, has extremely low viscosity and is similar to water. Thus, it can be easily applied underground by gravity feed or low pressure injection. After the reagent is injected, the reagent penetrates through the permeable water-containing layer area in the form of colloidal suspension, a thin layer of particles covers the pore structure, and the target pollutant is separated from the water phase and adsorbed on the colloidal activated carbon substrate. In addition, the degraded pollutants are dissociated from the biological matrix to release adsorption sites, so that continuous circulation is realized and in-situ regeneration is continued. Compared with the prior art, the invention has the advantages that:
(1) the colloidal activated carbon is injected into the aquifer polluted by the chlorohydrocarbon to repair the underground water, the flowing capacity of the colloidal activated carbon in the underground water is fully utilized, compared with other activated carbon, the colloidal activated carbon can also play a strengthened adsorption effect in the stratum with poor permeability, and can be injected into the stratum under the condition of no pressure or low pressure, so that the injection depth is greatly reduced, and the injection cost is reduced.
(2) The colloidal activated carbon has strong adsorption capacity, so that the colloidal activated carbon has a good effect on repairing low-concentration chlorinated hydrocarbon polluted sites, has a strong specific surface area and a loose structure, greatly enhances the adsorption capacity of pollutants, can degrade microorganisms to provide a place for survival and propagation, increases the diversity and activity of the microorganisms, and is more favorable for degrading chlorinated hydrocarbons.
(3) After the colloidal activated carbon is injected into a stratum, dissolved phase pollutants are separated from underground water and are concentrated on activated carbon particles, chlorohydrocarbon degrading bacteria are settled on the colloidal activated carbon to form a biological matrix, the pollutants in the biological matrix are biodegraded by the chlorohydrocarbon degrading bacteria to release adsorption sites, and thus a sustainable circulation removal mechanism of adsorption-degradation-reabsorption-redegradation is formed.
(4) The injection repairing work is carried out by utilizing the direct-push type injection system, an injection well does not need to be built, the injection repairing work is efficiently and quickly completed by means of the strong flexibility of the direct-push type drilling machine and the flowability of the colloidal activated carbon, the injection repairing work is used for greatly reducing the repairing cost of pollutants in different fields with different depths distributed in a layered mode, and the repairing period is shortened.
(5) The method is easy to operate, the used materials are all green and environment-friendly, secondary pollution to the environment is avoided, the medicament injection technology is mature, the application is convenient, and the method is economical and practical and can be widely popularized.
Drawings
FIG. 1 is the isothermal adsorption curve of TCE (trichloroethylene) in water for nano-colloidal activated carbon prepared in example 1 of the present invention;
FIG. 2 is a block diagram of an injection system of a direct drive drilling machine in accordance with embodiment 3 of the present invention;
FIG. 3 shows the location and distribution of injection points in a contaminated site in example 3 of the present invention;
FIG. 4 is a graph showing the change of TCE concentration with time in contaminated area 1 in example 3 of the present invention;
FIG. 5 is a graph showing the change of the concentration of 1,2-DCE in contaminated area 2 with time in example 3 of the present invention.
Detailed Description
The technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention.
Example 1
The preparation method of the nano-colloid activated carbon comprises the following specific steps:
(1) the specific surface area is 1050m250mg of coconut shell activated carbon with the grain diameter of 10 mu m;
(2) adding the coconut shell activated carbon into a horizontal ball mill pulverizer to grind for 96 hours to ensure that the particle size range is 0.1-1.8 mu m;
(3) adding 250mL of 65 ℃ distilled water into the ground activated carbon, placing the mixture into a high-pressure homogenizer for circulating and homogenizing for 10 times, wherein the homogenizing pressure is 80MPa, and naturally cooling to room temperature to obtain nano colloidal activated carbon with the particle size of 50-150 nm;
(4) 2.5mL, 5.0mL, 7.5mL, 12.5mL, 25mL, 37.5mL, 50mL, 62.5mL, 75mL of nano-colloidal activated carbon was added to nine 500mL adsorption reaction flasks, respectively;
(5) respectively adding 500mL of TCE water sample with the concentration of 2.5mg/L into nine 500mL reaction bottles;
(6) and (3) covering and sealing a bottle cap with a lining of silica gel/polytetrafluoroethylene, rotating the bottle cap on a rolling shaking table for 2 hours, and standing until the activated carbon is completely precipitated to obtain the nano colloidal activated carbon.
The prepared nano colloidal activated carbon is used for adsorbing TCE in water, so that the adsorption performance of the nano colloidal activated carbon is verified. Taking the supernatant of the step (6) to determine the equilibrium concentration of TCE, and the isothermal adsorption curve is shown in figure 1. As can be seen from the figure, when the experiment reaches the equilibrium, the equilibrium adsorption quantity of the nano-colloidal activated carbon to TCE is 270 mg/g.
Example 2
In this example, a colloidal activated carbon-based composite bacterium was prepared using the nano-colloidal activated carbon of example 1. The method comprises the following specific steps:
(1) preparing an inorganic culture medium: 1.650g of NH4NO3，1.9g KNO3，0.440g CaCl2·2H2O，0.370g MgSO4·7H2O，170mg KH2PO4，37.3mg Na2-EDTA，27.8mg FeSO4·7H2O，6.2mg H3BO3，22.3mg MnSO4·4H2O，8.6mg ZnSO4·7H2O，0.83mg KI，0.25mg Na2MoO·2H2O，0.025mg CuSO4·5H2O，0.025mg CoCl2·6H2Adding O into 1L deionized water, adjusting pH to 7.0 with 0.1M NaOH solution, sterilizing in autoclave at 121 deg.C for 20 min, and cooling in ultra-clean bench to obtain inorganic culture medium.
(2) Adding 8g of underground water polluted by chlorohydrocarbon into the inorganic culture medium in the step (1), oscillating at constant temperature and in a dark place at the temperature of 30 ℃ and the rotating speed of 180r/min, and culturing for 36 hours to obtain a culture bacterial liquid;
(2) taking 5mL of the culture solution obtained in the step (2), transferring the culture solution into a fresh liquid inorganic salt culture medium containing 10mg/L of chlorohydrocarbon and 0.15g/L of glucose, and performing acclimation culture until the solution is turbid to obtain an acclimation culture medium;
(3) taking 1mL of domesticated culture medium, diluting the culture solution obtained in the step (2) into 10 percent by a 10-fold dilution method-1-10-7Coating 0.1mL of gradient bacterial suspension on the inorganic culture medium in the step (1), uniformly coating by using a sterile coater, placing a culture dish in a biochemical incubator at 30 ℃ for culture and observation, then finding that obvious bacterial colonies appear, selecting the bacterial colonies, inoculating the bacterial colonies into the culture medium, and culturing in an oscillator at the temperature of 30 ℃ and the rotating speed of 180r/min to obtain the efficient chlorohydrocarbon degradation microbial inoculum;
(4) mixing the efficient bacterial liquid chlorohydrocarbon degrading microbial inoculum and the colloidal active carbon prepared in the embodiment 1 in a ratio of 1: 19 to obtain the nano colloid activated carbon-based composite bacteria.
Example 3
For a certain industrial site, two chlorinated solvent pollution plumes are monitored below the industrial site, and main pollution factors are trichloroethylene and 1, 2-dichloroethylene. The nano-colloid activated carbon-based composite bacteria solution prepared in the embodiment 2 of the invention is used for repairing underground water of a polluted site by adopting a direct injection system.
The direct-pushing injection system comprises a microbial inoculum preparation system, an injection pump system and a direct-pushing injection system, wherein the microbial inoculum preparation system transports a mixed microbial inoculum into a microbial inoculum barrel through a diaphragm pump, and then the mixed microbial inoculum is subjected to preparation work of dissolution, dilution and stirring through a microbial inoculum stirrer; the injection pump system provides power through a hydraulic system of the drilling machine, the mixed microbial inoculum is pressurized and pushed into a pressure pipeline, an injection drill rod and a drill bit are pushed into an injection point through a direct-pushing injection system of the drilling machine for injection, and the structure of the direct-pushing injection system is shown in figure 2.
The water level of underground water in the land is 2m, the pollutant in a polluted area 1 is trichloroethylene according to early investigation, the highest concentration is 1260 mu g/L, the thickness of a water-bearing layer is 8m, and the underground water is composed of fine sand and silt; the pollutant in the polluted area 2 is 1, 2-dichloroethylene, the maximum concentration is 86.3 mu g/L, the thickness of an aquifer is 2.6m, and the aquifer is composed of sand with higher permeability.
In the restoration range of the polluted area 1, injection points are arranged every 3m away from pollutants along the downstream direction of the groundwater flow, the position arrangement and restoration range of the injection points are shown in figure 3, a direct-push drilling machine with a medicament injection head is used for injecting medicaments, the injection pressure is 2MPa, the flow rate is 30L/min, and the slave capacity of the injection pump is 20m3The microbial inoculum storage tank sucks the colloidal activated carbon-based composite bacteria diluent described in embodiment 2, and the dilution times are 100 times. Injecting in a mode of 'from bottom to top', firstly pressing the injection head to the position 8m underground, lifting the injection head 2m upwards after completing the primary injection, and then performing the secondary injection until reaching 2m away from the ground surface. After completion, a second injection well is performed.
Within the repairing range of the polluted region 2, injection points are arranged at intervals of 5m away from pollutants along the downstream direction of the underground water flow, the position arrangement and repairing range of the injection points are shown in figure 3, and a direct-push drilling machine with a medicament injection head is used for carrying out medicament injectionInjecting with injection pump with injection pressure of 1.2MPa and flow rate of 30L/min, the injection pump has a volume of 20m3The microbial inoculum storage tank sucks the microbial inoculum. Each injection point adopts one-time injection, and the injection of the point is completed after the injection head is pressed to the position 2m below the ground for injection.
In order to master the content change of the pollutants in the field soil and the underground water after the microbial inoculum is injected, the monitoring well is monitored after the microbial inoculum is injected. As the flow of the groundwater in the field is from west to east, a monitoring well is arranged in a downstream repairing area of the groundwater pollution area for detecting the groundwater repairing condition. The well placement is shown in figure 3.
After the microbial inoculum is injected, a water sample is periodically collected from the observation well for analysis and test of the chlorohydrocarbon, so that the remediation effect of the underground water in the site is analyzed, and the change of the concentration of the chlorohydrocarbon in the underground water along with time is shown in the figures 4 and 5.
Therefore, the underground water chlorohydrocarbons are obviously reduced after the nano-colloid activated carbon-based composite bacteria in the example 3 are added. After trichloroethylene in the polluted area 1 is added into the composite bacteria for 60 days, the chlorohydrocarbon in the underground water is reduced to 608.3 mug/L from 1200 mug/L, and the detection value is zero after 270 days of injection; after the 1, 2-dichloroethylene in the polluted area 2 is added into the composite bacteria for 60 days, the chlorohydrocarbons in the underground water are reduced to 40.2 mu g/L from 86.3 mu g/L, and the detection value after 330 days of injection is zero, which shows that the nano colloid activated carbon-based composite bacteria have good repairing effects on the underground water pollution of high-concentration trichloroethylene and the underground water pollution of low-concentration 1, 2-dichloroethylene.
Claims (9)
1. An in-situ reinforced repair method for underground water polluted by chlorohydrocarbon is characterized by comprising the following steps:
step 1, investigating hydrogeology and pollutant distribution conditions of a polluted site;
step 2, calculating the size of the pollution plume of the underground water, and defining a pollution area according to the size of the pollution plume;
step 3, preparing nano colloid activated carbon-based composite bacteria, and mixing the nano colloid activated carbon-based composite bacteria with water according to the proportion of 1: 10-1: 1000 to prepare a mixed microbial inoculum;
and 4, establishing injection points in each pollution area, pushing the mixed microbial inoculum to a target pollution layer through a direct-pushing drilling machine injection system, and sequentially injecting the mixed microbial inoculum into each injection point under certain pressure.
2. The method of claim 1, wherein a long-term monitoring well is established downstream of the polluted area, and sampling monitoring after remediation is performed periodically to evaluate the remediation effect.
3. The in-situ reinforced repair method for underground water polluted by chlorinated hydrocarbons according to claim 1, wherein the specific steps for preparing the nano-colloid activated carbon-based composite bacteria in the step 3 are as follows:
step 31, grinding the solid activated carbon to ensure that the particle size range is 0.1-1.8 mu m;
step 32, mixing the ground activated carbon into distilled water at 65 ℃, homogenizing for many times, and naturally cooling to room temperature to obtain stable nano colloidal activated carbon uniformly suspended in water;
step 33, mixing the efficient chlorohydrocarbon degradation microbial inoculum and the nano colloidal activated carbon matrix prepared in the step 32 in a ratio of 1: 19 are mixed uniformly.
4. The method for in-situ reinforced remediation of underground water polluted by chlorinated hydrocarbons according to claim 3, wherein the high-efficiency chlorinated hydrocarbon degrading microbial inoculum in the step 33 is screened by the following process: collecting an underground water sample polluted by chlorohydrocarbon; diluting and coating an underground water sample on a chlorohydrocarbon-containing plate culture medium to culture colonies; and (3) carrying out streak separation and purification, and repeatedly domesticating the purified strain on a fresh culture medium plate containing the chlorohydrocarbon for many times to obtain the efficient chlorohydrocarbon degrading microbial inoculum.
5. The in-situ reinforced repair method for underground water polluted by chlorinated hydrocarbon according to claim 4, wherein the culture medium containing chlorinated hydrocarbon comprises the following components in parts by weight: the ratio of the liquid inorganic salt culture medium to the chlorinated hydrocarbon polluted underground water sample is 125: 1.
6. The method of claim 4, wherein the liquid inorganic salt medium comprises: NH (NH)4NO3:1650mg/L，KNO3:1900mg/L，CaCl2·2H2O:440mg/L，MgSO4·7H2O:370mg/L，KH2PO4:170mg/L，Na2-EDTA:37.3mg/L，FeSO4·7H2O:27.8mg/L，H3BO3:6.2mg/L，MnSO4·4H2O:22.3mg/L，ZnSO4·7H2O:8.6mg/L，KI:0.83mg/L，Na2MoO·2H2O:0.25mg/L，CuSO4·5H2O:0.025mg/L，CoCl2·6H2O:0.025mg/L。
7. The in-situ reinforced repair method for underground water polluted by hydrochloric ether according to claim 3, wherein the activated carbon in the step 31 is one of shaddock peel activated carbon, coconut shell activated carbon or corn stalks, and the specific surface area of the activated carbon is more than 1050 square meters per gram.
8. The in-situ reinforced repair method for underground water polluted by chlorinated hydrocarbon according to claim 3, wherein the particle size of the nano colloidal activated carbon in the step 32 is 10-180 nm.
9. The in-situ reinforced repair method for underground water polluted by chlorinated hydrocarbons according to claim 1, wherein in the step 4, the injection system of the direct-push drilling machine comprises a microbial inoculum preparation system, an injection pump system and a direct-push injection system, and the microbial inoculum preparation system is used for carrying out preparation work of dissolving, diluting and stirring the nano-colloid activated carbon-based composite bacteria through a microbial inoculum stirrer; the injection pump system provides power through a hydraulic system of the drilling machine, boosts and propels the mixed microbial inoculum to a pressure pipeline, and then propels the injection drill rod and the drill bit to an injection point through a direct-pushing injection system of the drilling machine for injection.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116274312A (en) * | 2022-10-17 | 2023-06-23 | 中国科学院南京土壤研究所 | Method for in-situ restoration and control of polluted site through permeable reactive barrier |
| CN117290410A (en) * | 2023-11-21 | 2023-12-26 | 江苏旭龙环境科技有限公司 | Environment monitoring method and system based on chlorinated hydrocarbon distribution prediction |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040007524A1 (en) * | 2002-07-12 | 2004-01-15 | Scott Noland | Compositions for removing hydrocarbons and halogenated hydrocarbons from contaminated environments |
| CN107309268A (en) * | 2017-08-02 | 2017-11-03 | 环境保护部南京环境科学研究所 | A kind of nanometer ferron injection device in situ and method for implanting |
| CN109052656A (en) * | 2018-07-13 | 2018-12-21 | 中国地质科学院水文地质环境地质研究所 | A kind of activity powdered carbon-degradation bacteria composition and groundwater remediation technology |
| CN110355193A (en) * | 2019-07-19 | 2019-10-22 | 中国科学院南京土壤研究所 | A kind of contaminated site in-situ remediation method based on dynamic ground water circulation |
-
2021
- 2021-01-12 CN CN202110035866.6A patent/CN112875874A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040007524A1 (en) * | 2002-07-12 | 2004-01-15 | Scott Noland | Compositions for removing hydrocarbons and halogenated hydrocarbons from contaminated environments |
| CN107309268A (en) * | 2017-08-02 | 2017-11-03 | 环境保护部南京环境科学研究所 | A kind of nanometer ferron injection device in situ and method for implanting |
| CN109052656A (en) * | 2018-07-13 | 2018-12-21 | 中国地质科学院水文地质环境地质研究所 | A kind of activity powdered carbon-degradation bacteria composition and groundwater remediation technology |
| CN110355193A (en) * | 2019-07-19 | 2019-10-22 | 中国科学院南京土壤研究所 | A kind of contaminated site in-situ remediation method based on dynamic ground water circulation |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116274312A (en) * | 2022-10-17 | 2023-06-23 | 中国科学院南京土壤研究所 | Method for in-situ restoration and control of polluted site through permeable reactive barrier |
| CN117290410A (en) * | 2023-11-21 | 2023-12-26 | 江苏旭龙环境科技有限公司 | Environment monitoring method and system based on chlorinated hydrocarbon distribution prediction |
| CN117290410B (en) * | 2023-11-21 | 2024-03-19 | 江苏旭龙环境科技有限公司 | Environment monitoring method and system based on chlorinated hydrocarbon distribution prediction |
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